Faculty Profiles - OZAKI Toshifumi

Papers - OZAKI Toshifumi

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ScleraxisとSox9による軟骨細胞分化調節

古松毅之, 迫間巧将, 吉田晶, 伊達宏和, 鉄永智紀, 雑賀建多, 阿部信寛, 宿南知佐, 雨宮三千代, 島野仁, 尾崎敏文

日本整形外科学会雑誌 82 ( 8 ) S1098 - S1098 2008.8

滑膜肉腫関連蛋白の細胞内局在に影響を与える薬剤および蛋白に関する検討

米田泰史, 大内田守, 神崎浩孝, 吉田晶, 佐々木剛, 森本裕樹, 国定俊之, 清水憲二, 尾崎敏文

日本整形外科学会雑誌 82 ( 8 ) S1023 - S1023 2008.8

ヒストン脱アセチル化酵素阻害剤とDNA脱メチル化剤の併用による滑膜肉腫細胞株に対する抗腫瘍効果の検討

佐藤千明, 吉田晶, 森本裕樹, 米田泰史, 佐々木剛, 国定俊之, 大内田守, 尾崎敏文

日本整形外科学会雑誌 82 ( 8 ) S1228 - S1228 2008.8

骨肉腫患者における葉酸代謝関連遺伝子の一塩基多型の検討

森本裕樹, 吉田晶, 大内田守, 米田泰史, 国定俊之, 尾崎敏文

日本整形外科学会雑誌 82 ( 8 ) S926 - S926 2008.8

Validation of radiographic response evaluation criteria of preoperative chemotherapy for bone and soft tissue sarcomas: Japanese Orthopaedic Association Committee on Musculoskeletal Tumors Cooperative Study. Reviewed

Ueda T, Naka N, Araki N, Ishii T, Tsuchiya H, Yoshikawa H, Mochizuki K, Tsuboyama T, Toguchida J, Ozaki T, Murata H, Kudawara I, Tanaka K, Iwamoto Y, Yazawa Y, Kushida K, Otsuka T, Sato K

Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 13 ( 4 ) 304 - 312 2008.7

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: The radiographic evaluation of the response to preoperative chemotherapy for bone and soft tissue sarcomas is based mostly on the change in primary tumor size before and after chemotherapy, as is done for many solid cancers. Its prognostic correlation, however, has hardly been validated. METHODS: We conducted a retrospective validation study of the Japanese Orthopaedic Association (JOA) radiographic response evaluation criteria of preoperative chemotherapy for bone and soft tissue sarcomas as a JOA Committee on Musculoskeletal Tumors cooperative study. A total of 125 consecutive patients with high-grade bone (n = 77) and soft tissue (n = 48) sarcomas treated with neoadjuvant chemotherapy and definitive surgery in 25 tertiary referral hospitals were selected for the study. We investigated the correlation between the tumor size-based radiographic response evaluation criteria of preoperative chemotherapy for bone and soft tissue sarcomas provided by the JOA Committee on Musculoskeletal Tumors (hereafter called the JOA criteria) and the patients' overall survival using the Kaplan-Meier method and the log-rank test. RESULTS: The JOA criteria correlated relatively well with survival for malignant bone tumors (mostly comprising osteosarcoma and Ewing's sarcoma) but not for soft tissue sarcomas, suggesting that the tumor size-based radiographic evaluation criteria for the response to preoperative chemotherapy in patients with soft tissue sarcomas is invalid. CONCLUSIONS: The JOA criteria, based on the change in primary tumor size, is valid for malignant bone tumors but invalid for soft tissue sarcomas. Other new evaluation modalities of the response to preoperative chemotherapy using innovative functional imaging techniques are needed for soft tissue sarcomas.

DOI： 10.1007/s00776-008-1235-5

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Single nucleotide polymorphism in reuced folate carrier-1 gene and methyleneterathydrofolate reductase gene in patients with osteosarcoma

Y. Morimoto, A. Yoshida, M. Ouchida, T. Kunisada, K. Shimizu, T. Ozaki

EJC SUPPLEMENTS 6 ( 9 ) 117 - 118 2008.7

橋本病を合併したMazabraud syndromeの1例

大畑範英, 森本裕樹, 吉田晶, 佐々木剛, 米田泰史, 国定俊之, 大内田守, 尾崎敏文

日本整形外科学会雑誌 82 ( 6 ) S853 - S853 2008.6

Interleukin-4 downregulates the cyclic tensile stress-induced matrix metalloproteinases-13 and cathepsin B expression by rat normal chondrocytes Reviewed

Hideyuki Doi, Keiichiro Nishida, Masanori Yorimitsu, Takamitsu Komiyama, Yasutaka Kadota, Tomonori Tetsunaga, Aki Yoshida, Satoshi Kubota, Masaharu Takigawa, Toshifumi Ozaki

ACTA MEDICA OKAYAMA 62 ( 2 ) 119 - 126 2008.4

Language：English Publishing type：Research paper (scientific journal) Publisher：OKAYAMA UNIV MED SCHOOL

Mechanical stress plays a key role in the pathogenesis of cartilage destruction seen in osteoarthritis (OA). We investigated the effect of cyclic tensile stress (CTS) on the anabolic and catabolic gene expression of rat cultured normal chondrocytes using the Flexercell strain unit. The effects of interleukin (IL)-4, a chondroprotective cytokine, on the changes in gene expression induced by CTS were also investigated. CTS (7% elongation at 0.5 Hz) for 24 h did not affect the expression of aggrecan and type 11 collagen, whereas CTS significantly upregulated matrix metalloproteinase (MMP)-13 and cathepsin B mRNA expression by chondrocytes. IL-1 beta expression was also significantly upregulated by CTS up to 12 h. The upregulation of MMP-13 was observed at 3 h, which was earlier than that of IL-1 beta. Furthermore, pre-treatment with IL-4 (10 ng/ml) suppressed both MMP-13 and cathepsin B induction by mechanical stress, as well as CTS-induced IL-1 beta expression. Our results suggest that IL-4 might have a therapeutic value in the treatment of OA by downregulation of mechanical stress-induced MMP-13 and cathepsin B expression by chondrocytes.

DOI： 10.18926/AMO/30956

Web of Science

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骨肉腫における6q16-23の高頻度染色体欠失の研究(High frequent allelic loss of chromosome 6q16-23 in osteosarcoma: Involvement of cyclin C in osteosarcoma)

大畑範英, 吉田晶, 伊藤佐智夫, 国定俊之, 森本裕樹, 神崎浩孝, 尾崎敏文, 清水憲二, 大内田守

日本癌学会総会記事 66回 345 - 345 2007.8

滑膜肉腫特異的転座型融合蛋白SS18-SSXおよびSS18、SSX蛋白の細胞内局在の解析

米田泰史, 大内田守, 神崎浩孝, 国定俊之, 清水憲二, 尾崎敏文

日本整形外科学会雑誌 81 ( 8 ) S1119 - S1119 2007.8

運動器リハビリテーションと健康寿命健康寿命の阻害因子としての運動器不安定症

千田益生, 堅山佳美, 濱田全紀, 迫間巧将, 那須巧, 尾崎敏文

日本整形外科学会雑誌 81 ( 4 ) S511 - S511 2007.4

Desmoid-type fibromatosisに対するTI-201シンチグラフィの検討

新家崇義, 赤木史郎, 佐藤修平, 尾形毅, 岡村淳, 郷原英夫, 加藤勝也, 清哲朗, 金澤右, 尾崎敏文

日本医学放射線学会学術集会抄録集 66回 S312 - S312 2007.2

4333 Estimation of registration error distribution from bone surfaces

SAITO Toki, NAKAJIMA Yoshikazu, SUGITA Naohiko, FUJIWARA Kazuo, ABE Nobuhiro, OZAKI Toshifumi, SUZUKI Masahiko, MORIYA Hideshige, INOUE Takayuki, KURAMOTO Koichi, NAKASHIMA Yoshio, MITSUISHI Mamoru

The proceedings of the JSME annual meeting 2007 519 - 520 2007

Language：Japanese Publisher：The Japan Society of Mechanical Engineers

Registration is a technique for corresponding between a pre-operative planning space and an intra-operative surgical space. It is often used in computer-integrated surgical systems, such as surgical navigation systems and surgical robots. One of most foundational registration methods is point-pair registration. We propose a method for estimation of most appropriate sampling-point set based on bone-surface shape analysis. The method consists of two steps; the first step is an estimation of point sampling error distribution from the bone surface, and the next one is an optimization of sampling-point set by using a computer simulation of point-pair registration. The result shows the effectiveness of this method.

DOI： 10.1299/jsmemecjo.2007.5.0_519

CiNii Article

CiNii Books

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Bone cutting robot with soft tissue collision avoidance capability by a redundant axis for minimally invasive orthopedic surgery Reviewed

Sugita N, Genma F, Nakajima Y, Mitsuishi M, Fujiwara K, Abe N, Ozaki T, Suzuki M, Moriya H, Inoue T, Kuramoto K, Nakashima Y, Tanimoto K

2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 48 - 51 2007

Publishing type：Research paper (international conference proceedings)

Many of the robots developed so far, including our multi-axis bone cutting robot, use an end mill as the cutting tool, and some problems should be solved to apply them to the minimally invasive orthopedic surgery. Minimally invasive surgery makes the incisions smaller, reduces pain and trauma to the body, and enables faster recovery. The smaller incision means a small and narrow opening area. This means the robot attitude for the bone resection is restricted, and it can result in the collision of the tool with the surrounding tissue, the existence of an untouched area and the degradation of the joint position accuracy. This paper proposes a robot mechanism and a toolpath generation technique specialized for bone cutting is expected to resolve these issues. © 2007 IEEE.

DOI： 10.1109/ICCME.2007.4381690

Scopus

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Other Link： http://orcid.org/0000-0002-1369-0747

Assessment of head wear more than ten years after total hip arthroplasty: 22-mm zirconia vs. metal heads.

Atsushi Inoue, Koji Asaumi, Hirosuke Endo, Kazuo Fujiwara, Shigeru Mitani, Toshifumi Ozaki

Acta medica Okayama 60 ( 6 ) 311 - 8 2006.12

Language：English Publishing type：Research paper (scientific journal)

The present retrospective study assessed radiographs to determine socket wear in total hip arthroplasty (THA) with 22-mm zirconia or COP (Cobalt-Chrome alloy rich in Cobalt and Phosphorous) heads, and in cemented stems at more than 10 years after operation. Sockets of ultra high molecular weight polyethylene were used in each of two THA groups (13 hips each) in a clinical trial in our hospital between 1989 and 1990. Three observers carried out masked assessments of the radiographs. Upon final examination, there was no remarkable loosening in the zirconia or COP group, and no case had required revision surgery as of 2005. There was a statistically significant difference between the 2 groups in average annual linear wear, at 0.093 mm/year and 0.046 mm/year in the zirconia and COP groups, respectively. Volume wear and average annual volume wear were also significantly greater in the zirconia group despite its superior mechanical strength and toughness in vitro. Our present findings do not confirm early expectations of lower wear in long-term results of 22-mm zirconia femoral heads used in THA.

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Highly frequent allelic loss of chromosome 6q16-23 in osteosarcoma: involvement of cyclin C in osteosarcoma. International journal

Norihide Ohata, Sachio Ito, Aki Yoshida, Toshiyuki Kunisada, Kunihiko Numoto, Yoshimi Jitsumori, Hirotaka Kanzaki, Toshifumi Ozaki, Kenji Shimizu, Mamoru Ouchida

International journal of molecular medicine 18 ( 6 ) 1153 - 8 2006.12

Language：English Publishing type：Research paper (scientific journal)

The molecular pathogenesis of osteosarcoma is very complicated and associated with chaotic abnormalities on many chromosomal arms. We analyzed 12 cases of osteosarcomas with comparative genomic hybridization (CGH) to identify chromosomal imbalances, and detected highly frequent chromosomal alterations in chromosome 6q, 8p, 10p and 10q. To define the narrow rearranged region on chromosome 6 with higher resolution, loss of heterozygosity (LOH) analysis was performed with 21 microsatellite markers. Out of 31 cases, 23 cases (74%) showed allelic loss at least with one marker on chromosome 6q. We identified two distinct commonly deleted regions on chromosome 6 using markers D6S1565 located at 6q16 and 6q23MS1 at 6q23. The expression analysis of genes located at the deleted region was performed, and the decreased mRNA expression of the CCNC gene, one of the regulators of cell cycle, was detected. Growth of osteosarcoma cell line was significantly suppressed after the CCNC cDNA transfection. Fine mapping of the deleted region containing a possible tumor suppressor gene and the transfection assay suggest that the CCNC is a candidate tumor suppressor gene.

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Expression of WISP-1 (ccn4), WISP-2 (ccn5) and WISP-3 (ccn6) in Rheumatoid Arthritic Synovium Evaluated by DNA Microarrays

Mori Hiroki, Nishida Keiichiro, Ozaki Toshifumi, Inoue Hajime, Setsu Kojun, Tsujigiwa Hidetsugu, Nagatsuka Hitoshi, Gunduz Mehmet, Nakanishi Tohru

Journal of hard tissue biology 15 ( 3 ) 89 - 95 2006.12

Language：English Publisher：The Society of Hard Tissue Biology

Rheumatoid disease (RA) is one of the complex diseases that showed multiple progression stages. At first inflammatory response is occurred by self antibodies, and then the proliferation of synoviocytes is abnormally promoted. These synoviocytes invade into articular structures and finally articular cartilage is destroyed. In order to analyze growth-promoting factors in synovial cells from articular tissues of RA, we performed DNA microarray analysis and compared the expression patterns of their mRNA to that of synoviocytes from osteoarthritis (OA). The results showed that the expression of many genes was up-regulated in RA synoviocytes comparing with OA synoviocytes. Among these genes, RT-PCR analysis revealed that Wnt-1-induced secreted protein 2 (WISP-2, CCN5) and protein 3 (WISP-3, CCN6) which are belong to the novel gene family, CCN, were highly up-regulated in RA synoviocytes. In addition, one truncated isoform of WISP-3 which may be related to the ongoing of some type of RA, was detected in RA synoviocytes. Furthermore, immunohistochemical analysis showed that the products of both CCN genes were highly distributed in active synoviocytes of articular tissues of RA. These results indicate that both CCN genes and their products play some roles in the progression of RA.

DOI： 10.2485/jhtb.15.89

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Other Link： http://search.jamas.or.jp/link/ui/2007191489

軟部腫瘍のTlシンチグラフィ疑陽性例の検討

赤木史郎, 新家崇義, 郷原英夫, 佐藤修平, 黒田昌宏, 金澤右, 井谷智, 国定俊之, 尾崎敏文

核医学 43 ( 4 ) 354 - 354 2006.11

Expression of neurotrophins and their receptors tropomyosin-related kinases (Trk) under tension-stress during distraction osteogenesis.

Ayako Aiga, Koji Asaumi, You-Jin Lee, Hiroaki Kadota, Shigeru Mitani, Toshifumi Ozaki, Masaharu Takigawa

Acta medica Okayama 60 ( 5 ) 267 - 77 2006.10

Language：English Publishing type：Research paper (scientific journal)

The localization and expression of neurotrophins and their receptors during distraction osteogenesis was investigated in 72 male rat femurs (11 weeks old) to further clarify the concurrence of cellular and molecular events of new bone formation. After osteotomy, a 7-day lag phase was followed by distraction at the rate of 0.25 mm/12 h for 21 days (distraction phase), and a 7-day consolidation phase. The localization of neurotrophins (NGF, BDNF and NT-3) and their receptors tropomyosinrelated kinases (TRKA, TRKB and TRKC) by immunostaining showed positive staining in bone forming cells in each stage, although the presence and staining intensity varied by cell type and phase. The expressions of NGF, BDNF and NT-3 by real-time polymerase chain reaction (real-time PCR) showed that the peak of the mRNA expression of NGF occurred 10 days after distraction. NT-3 increased during bone extension, but decreased when distraction stopped. In contrast, BDNF continued to increase gradually throughout the distraction and consolidation phases. These findings suggest that neurotrophins and their receptors may play different roles in endochondral and intramembranous ossification in distraction osteogenesis. The tension stress caused by distraction may stimulate the expression of neurotrophins and their receptors, and promote osteogenesis.

PubMed

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骨形成不全症の骨折に対する治療戦略

皆川寛, 三谷茂, 遠藤裕介, 鉄永智紀, 黒田崇之, 尾崎敏文

日本整形外科学会雑誌 80 ( 10 ) 710 - 710 2006.10

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Papers - OZAKI Toshifumi