Faculty Profiles - OZAKI Toshifumi

【目的】ヒト靱帯の断面積計測法については多くの報告があるが,膝の生理的な動きに伴うin situでの断面積を計測した報告はない.今回我々はロボットシステムを用いて再現した膝の生理的な動きの中での後十字靱帯(PCL)の断面積変化を調べた.【方法】ヒト屍体膝10体を対象とし,ロボットシステムを用いてあらかじめ記録した屈曲0,30,60,90°におけるneutral position,89N脛骨後方引出力負荷下(posterior tibial translation;PTT)の膝の動きを再現した.FAROレーザースキャナを用いて各角度,負荷下でのPCLの三次元画像を構築し,Geomagicソフトを使用し画像解析を行った.PCL実質部に適合するbest fit cylinderを作成し,それに垂直な面上での断面積を計測した.断面積計測はPCL実質部の全長を5等分した面で行い,膝屈曲30°でのneutral positionとPTTにおける断面積の変化を評価した.また,膝屈伸に伴うPCL実質部中央での断面積の変化を評価した.【結果】Neutral position,PTTにおいてPCL断面積は中央部で最小であった.全ての部位においてPTTにおける断面積がneutral positionのそれに比べ有意に小さかった.PCL中央部での断面積は膝屈曲0°で最大で90°で最小であった.全角度においてneutral positionに比べPTTにおける断面積が有意に小さかった.【考察および結論】本研究は,膝の動きに伴うin situでのPCLの断面積変化を正確に計測した初めての研究である.PCL断面積は中央部で最も小さく,どの部位においても膝屈曲とPTTにより断面積が小さくなることがわかった.(著者抄録)

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寛骨臼骨折におけるPosterior Column ScrewおよびInfra Acetabular Screwの安全域 3DCTによる検討

上原健敬, 野田知之, 吉村将秀, 杉生和久, 山川泰明, 雑賀建多, 中原龍一, 島村安則, 木浪陽, 尾崎敏文

骨折 37 ( Suppl. ) S188 - S188 2015.6

The figure-of-nine leg position for anatomic anterior cruciate ligament reconstruction Reviewed

T. Furumatsu, M. Fujii, T. Tanaka, S. Miyazawa, T. Ozaki

ORTHOPAEDICS & TRAUMATOLOGY-SURGERY & RESEARCH 101 ( 3 ) 391 - 393 2015.5

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER MASSON

Anatomic double-bundle anterior cruciate ligament (ACL) reconstruction can restore the function and kinematics of the knee in ACL-deficient patients. Several outside-in drilling systems for accurate femoral tunnel creations have been developed. However, the femoral tunnel creation at the lower position of the intercondylar notch can be difficult in a usual leg position with the knee flexed at 90 degrees without varus stress. This technical note describes that the figure-of-nine leg position provides a better arthroscopic view to safely clean up the ACL femoral footprint located at the lower area of the lateral intercondylar wall. This position is useful to create the optimal femoral tunnels using the outside-in drilling technique, without damaging the lateral meniscus posterior root, lateral tibial eminence, and supplemental fibers that bridge the gap between the lateral meniscus and the ACL tibial insertion. (C) 2015 Elsevier Masson SAS. All rights reserved.

DOI： 10.1016/j.otsr.2014.12.015

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高度肥満症例の重度四肢骨盤外傷に対する骨折治療戦略

小松原将, 木浪陽, 上原健敬, 山川泰明, 野田知之, 尾崎敏文

日本外傷学会雑誌 29 ( 2 ) 281 - 281 2015.5

骨盤輪骨折に対するナビゲーションを用いたiliosacral screwの有用性

上原健敬, 木浪陽, 吉村政秀, 杉生和久, 山川泰明, 野田知之, 尾崎敏文

日本外傷学会雑誌 29 ( 2 ) 234 - 234 2015.5

健常人の横隔神経伝導検査における左右差の検討

堅山佳美, 千田益生, 原田遼三, 上原健敬, 西田圭一郎, 尾崎敏文

The Japanese Journal of Rehabilitation Medicine 52 ( Suppl. ) S198 - S198 2015.5

Intercondylar notch size influences cyclops formation after anterior cruciate ligament reconstruction Reviewed

Masataka Fujii, Takayuki Furumatsu, Shinichi Miyazawa, Yukimasa Okada, Takaaki Tanaka, Toshifumi Ozaki, Nobuhiro Abe

KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY 23 ( 4 ) 1092 - 1099 2015.4

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

The purpose of this study was to investigate the incidence of cyclops lesions and its relationship with the cross-sectional area of the intercondylar notch.
For this study, 55 patients (24 male and 31 female) underwent follow-up arthroscopy after bi-socket anterior cruciate ligament reconstruction with hamstring tendon grafts were included. All patients underwent magnetic resonance imaging measurements of intercondylar notch dimensions. We compared the femoral intercondylar notch sizes and bone tunnel sizes between knees with cyclops lesions (cyclops group) and those without cyclops lesions (no-cyclops group). The mean percentage of the tunnel size to the cross-sectional area of the femoral intercondylar notch was also compared between the groups. The median follow-up duration was 3.8 years.
Cyclops lesions were found in 15 of the 55 knees (27.3 %) on second-look arthroscopy (cyclops group). Only 6 of the 55 knees (10.9 %) had extension loss (cyclops syndrome). The cyclops group included 3 men and 12 women. The two groups showed a statistical difference in sex variation (P = 0.04). No significant differences were found in the femoral and tibial tunnel sizes between the two groups. The cross-sectional area of the femoral intercondylar notch was significantly smaller in the cyclops group (251.7 +/- A 63.2 mm(2)) than in the no-cyclops group (335.6 +/- A 77.6 mm(2)) (P < 0.001). The percentage of the total femoral tunnel size to the cross-sectional area of the femoral intercondylar notch was significantly higher in the cyclops group (18.6 +/- A 5.3 %) than in the no-cyclops group (13.2 +/- A 3.6 %) (P = 0.02).
A smaller intercondylar notch size may be a potential risk factor for cyclops lesion formation.

DOI： 10.1007/s00167-014-2891-y

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Pneumocephalus and chylothorax complicating vertebrectomy for lung cancer. Reviewed International journal

Sugimoto S, Tanaka M, Suzawa K, Nishikawa H, Toyooka S, Oto T, Ozaki T, Miyoshi S

The Annals of thoracic surgery 99 ( 4 ) 1425 - 1428 2015.4

Language：English Publishing type：Research paper (scientific journal)

Pneumocephalus is a rare, but potentially fatal complication of thoracic surgery. We describe a case of successful management of pneumocephalus complicated by persistent chylothorax developing after en bloc partial vertebrectomy performed after induction chemoradiotherapy for lung cancer invading the spine. Surgical treatment should be considered for pneumocephalus complicated by any condition requiring persistent chest drainage.

DOI： 10.1016/j.athoracsur.2014.05.098

PubMed

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Inhibition of KRAS codon 12 mutants using a novel DNA-alkylating pyrrole-imidazole polyamide conjugate. Reviewed International journal

Hiraoka K, Inoue T, Taylor RD, Watanabe T, Koshikawa N, Yoda H, Shinohara K, Takatori A, Sugimoto H, Maru Y, Denda T, Fujiwara K, Balmain A, Ozaki T, Bando T, Sugiyama H, Nagase H

Nature communications 6 6706 - 6706 2015.4

Language：English Publishing type：Research paper (scientific journal)

Despite extensive efforts to target mutated RAS proteins, anticancer agents capable of selectively killing tumour cells harbouring KRAS mutations have remained unavailable. Here we demonstrate the direct targeting of KRAS mutant DNA using a synthetic alkylating agent (pyrrole-imidazole polyamide indole-seco-CBI conjugate; KR12) that selectively recognizes oncogenic codon 12 KRAS mutations. KR12 alkylates adenine N3 at the target sequence, causing strand cleavage and growth suppression in human colon cancer cells with G12D or G12V mutations, thus inducing senescence and apoptosis. In xenograft models, KR12 infusions induce significant tumour growth suppression, with low host toxicity in KRAS-mutated but not wild-type tumours. This newly developed approach may be applicable to the targeting of other mutant driver oncogenes in human tumours.

DOI： 10.1038/ncomms7706

PubMed

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Hyaluronan suppresses mechanical stress-induced expression of catabolic enzymes by human chondrocytes via inhibition of IL-1 beta production and subsequent NF-kappa B activation Reviewed

Masatsugu Ozawa, Keiichiro Nishida, Aki Yoshida, Taichi Saito, Ryozo Harada, Takahiro Machida, Toshifumi Ozaki

INFLAMMATION RESEARCH 64 ( 3-4 ) 243 - 252 2015.4

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER BASEL AG

To investigate the inhibitory effect of hyaluronan (HA) on mechanical stress- induced expression of a disintegrin and metalloproteinase with thrombospondin type1 motifs (ADAMTS)-4, -5 and matrix metalloproteinase (MMP)-13 by human chondrocytes.
Normal human articular chondrocytes were pre-incubated with or without 1.0 mg/mL HA (2700 kDa) for 12 h at 37 A degrees C in stretch chambers, then they were exposed to uni-axial cyclic tensile strain (CTS, 0.5 Hz, 10 % elongation). The expression of ADAMTS-4, -5, and MMP-13 were analyzed by real-time polymerase chain reaction and Immunocytochemistry. The concentration of IL-1 beta in the supernatant was measured using enzyme-linked immunosorbent assay (ELISA). The nuclear translocation of runt-related transcription factor 2 (RUNX-2) and nuclear factor-kappa B (NF-kappa B) was examined by ELISA and immunocytochemistry, and phosphorylation of NF-kappa B was examined by western blotting.
HA inhibited mRNA expression of ADAMTS-4, -5, and MMP13 after 24 h CTS via inhibition of IL-1 beta secretion and NF-kappa B activation. However, HA failed to inhibit CTS-induced RUNX-2 expression and subsequent expression of ADAMTS-5 and MMP-13 1 h after CTS.
Our results demonstrated that HA significantly suppressed mechanical stress-induced expression of catabolic proteases by inhibition of the NF-kappa B-IL-1 beta pathway, but did not suppress mechanical stress-induced RUNX-2 signaling.

DOI： 10.1007/s00011-015-0804-2

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3D-navigation systemを用いたtransiliac-transsacral screw固定の経験

木浪陽, 野田知之, 上原健敬, 山川泰明, 尾崎敏文

中国・四国整形外科学会雑誌 27 ( 1 ) 182 - 182 2015.4

不安定型骨盤輪骨折に対するnavigation systemを利用したIliosacral screwの有用性

上原健敬, 野田知之, 山川泰明, 井上円加, 中原龍一, 島村安則, 尾崎敏文, 木浪陽

中国・四国整形外科学会雑誌 27 ( 1 ) 173 - 174 2015.4

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