Faculty Profiles - OZAKI Toshifumi

Papers - OZAKI Toshifumi

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RNA干渉核酸医薬による骨肉腫がん幹細胞様形質の制御を基盤とした新規治療戦略

藤原智洋, 小坂展慶, 窪田大介, 川井章, 尾崎敏文, 落谷孝広

中部日本整形外科災害外科学会雑誌 56 ( 秋季学会 ) 174 - 174 2013.9

Transcription factor Mohawk and the pathogenesis of human anterior cruciate ligament degradation. International journal

Hiroyuki Nakahara, Akihiko Hasegawa, Koji Otabe, Fumiaki Ayabe, Tetsuya Matsukawa, Naoko Onizuka, Yoshiaki Ito, Toshifumi Ozaki, Martin K Lotz, Hiroshi Asahara

Arthritis and rheumatism 65 ( 8 ) 2081 - 9 2013.8

Language：English Publishing type：Research paper (scientific journal)

OBJECTIVE: To investigate the expression and function of Mohawk (MKX) in human adult anterior cruciate ligament (ACL) tissue and ligament cells from normal and osteoarthritis (OA)-affected knees. METHODS: Knee joints were obtained at autopsy (within 24-48 hours postmortem) from 13 donors with normal knees (mean ± SD age 36.9 ± 11.0 years), 16 donors with knee OA (age 79.7 ± 11.4 years), and 8 aging donors without knee OA (age 76.9 ± 12.9 years). All cartilage surfaces were graded macroscopically. MKX expression was analyzed by immunohistochemistry and quantitative polymerase chain reaction. ACL-derived cells were used to study regulation of MKX expression by interleukin-1β (IL-1β). MKX was knocked down with small interfering RNA (siRNA) to analyze the function of MKX in extracellular matrix (ECM) production and differentiation in ACL-derived cells. RESULTS: The expression of MKX was significantly decreased in ACL-derived cells from OA knees compared with normal knees. Consistent with this finding, immunohistochemistry analysis showed that MKX-positive cells were significantly reduced in ACL tissue from OA donors, in particular in cells located in disorientated fibers. In ACL-derived cells, IL-1β strongly suppressed MKX expression and reduced expression of the ligament ECM genes COL1A1 and TNXB. In contrast, SOX9, a chondrocyte master transcription factor, was up-regulated by IL-1β treatment. Importantly, knockdown of MKX expression with siRNA up-regulated SOX9 expression in ACL-derived cells, whereas the expression of COL1A1 and TNXB was reduced. CONCLUSION: Reduced expression of MKX is a feature of degenerated ACL in OA-affected joints, and this may be mediated in part by IL-1β. MKX appears necessary to maintain the tissue-specific cellular differentiation status and ECM production in adult human tendons and ligaments.

DOI： 10.1002/art.38020

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可視光硬化ゼラチンとcollagen binding domain(CBD)-BMP4の併用による家兎骨軟骨組織修復効果

馬崎哲朗, 塩崎泰之, 山根健太郎, 香川洋平, 篠原健介, 吉田晶, 松川昭博, 中村真理子, 吉田靖弘, 北嶋隆, 伊藤嘉浩, 尾崎敏文

日本整形外科学会雑誌 87 ( 8 ) S1606 - S1606 2013.8

可視光硬化型ゼラチンとコラーゲン結合性ドメイン連結成長因子を用いた脊髄損傷治療

篠原健介, 馬崎哲朗, 塩崎泰之, 吉田晶, 松川昭博, 尾崎敏文

日本整形外科学会雑誌 87 ( 8 ) S1440 - S1440 2013.8

家兎骨軟骨欠損モデルにおける可視光硬化ゼラチンおよび骨髄間質細胞の組織修復効果

香川洋平, 馬崎哲朗, 塩崎泰之, 山根健太郎, 吉田晶, 松川昭博, 中村真理子, 吉田靖弘, 北嶋隆, 伊藤嘉浩, 尾崎敏文

日本整形外科学会雑誌 87 ( 8 ) S1405 - S1405 2013.8

ケンフェロールが骨粗鬆症モデルマウスの骨吸収と骨組織に与える影響

吉田晶, 塩崎泰之, 馬場哲朗, 山根健太郎, 松川昭博, 北嶋隆, 伊藤嘉浩, 美野愛, 佐藤美和, 尾崎敏文

日本整形外科学会雑誌 87 ( 8 ) S1380 - S1380 2013.8

メカニカルストレスに対するヒアルロン酸の軟骨破壊抑制効果の機序の解析

小澤正嗣, 西田圭一郎, 橋詰謙三, 中原龍一, 斉藤太一, 金澤智子, 原田遼三, 町田崇博, 尾崎敏文

日本整形外科学会雑誌 87 ( 8 ) S1321 - S1321 2013.8

PROSNAP人工肘関節置換術の短期成績

那須義久, 西田圭一郎, 橋詰謙三, 中原龍一, 斎藤太一, 金澤智子, 小澤正嗣, 尾崎敏文

岡山医学会雑誌 125 ( 2 ) 183 - 184 2013.8

腫瘍由来エクソソームは骨肉腫の進展においてどのような役割を担うか

藤原智洋, 勝田毅, 小坂展慶, 川井章, 尾崎敏文, 落谷孝広

日本整形外科学会雑誌 87 ( 8 ) S1461 - S1461 2013.8

医学生の臨床実習充実化のために研修医が果たす役割岡山大学病院初期研修医の学生教育に対する意識調査

三好智子, 片岡仁美, 小比賀美香子, 渡辺文恵, 別府治恵, 森祐子, 山根正修, 松川昭博, 尾崎敏文, 谷本光音

医学教育 44 ( Suppl. ) 112 - 112 2013.7

第64回西日本医科学生総合体育大会の安全対策とその結果

森俊太, 高橋政史, 宮澤慎一, 阿部信寛, 古松毅之, 尾崎敏文

日本整形外科スポーツ医学会雑誌 33 ( 3 ) 308 - 315 2013.7

モジュラー型硬性胸腰仙椎装具の試作

堅山佳美, 千田益生, 馬崎哲朗, 上原健敬, 尾崎敏文

運動器リハビリテーション 24 ( 2 ) 149 - 149 2013.6

上腕三頭筋切除を要した軟部肉腫の術後患肢機能

武田健, 国定俊之, 長谷井嬢, 井上円加, 大森敏規, 上原健敬, 山川泰明, 尾崎修平, 吉田晶, 尾崎敏文

日本整形外科学会雑誌 87 ( 6 ) S1190 - S1190 2013.6

長期生存が得られた胸椎原発Ewing肉腫の2例

上原健敬, 国定俊之, 武田健, 長谷井嬢, 田中雅人, 尾崎敏文

日本整形外科学会雑誌 87 ( 6 ) S1170 - S1170 2013.6

脛骨骨幹部骨折に合併した腓骨骨折固定の重要性

山川泰明, 野田知之, 上原健敬, 井上円加, 金澤智子, 中原龍一, 木浪陽, 島村安則, 国定俊之, 尾崎敏文

骨折 35 ( Suppl. ) S350 - S350 2013.6

術中3Dナビゲーションを用いたISスクリュー固定術の経験

木浪陽, 野田知之, 山川泰明, 井上円加, 金澤智子, 斉藤太一, 中原龍一, 島村安則, 尾崎敏文

骨折 35 ( Suppl. ) S202 - S202 2013.6

上腕骨近位端骨折に骨幹部骨折を合併した症例にMIPO法によるdouble plate固定を施行した一例

原田遼三, 島村安則, 野田知之, 木浪陽, 中原龍一, 斉藤太一, 金澤智子, 井上円加, 山川泰明, 尾崎敏文

骨折 35 ( Suppl. ) S192 - S192 2013.6

骨肉腫がん幹細胞様性質に対するRNA干渉を応用した前臨床試験とその臨床的意義

藤原智洋, 小坂展慶, 高橋陵宇, 窪田大介, 近藤格, 川井章, 尾崎敏文, 落谷孝広

日本整形外科学会雑誌 87 ( 6 ) S1098 - S1098 2013.6

Tensile strain increases expression of CCN2 and COL2A1 by activating TGF-beta-Smad2/3 pathway in chondrocytic cells Reviewed

Takayuki Furumatsu, Emi Matsumoto, Tomoko Kanazawa, Masataka Fujii, Zhichao Lu, Ryotaro Kajiki, Toshifumi Ozaki

JOURNAL OF BIOMECHANICS 46 ( 9 ) 1508 - 1515 2013.5

Efficacy of low-intensity pulsed ultrasound treatment for surgically managed fresh diaphyseal fractures of the lower extremity: multi-center retrospective cohort study.

Yo Kinami, Tomoyuki Noda, Toshifumi Ozaki

Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 18 ( 3 ) 410 - 8 2013.5

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: There are no evidence on the effects of low-intensity pulsed ultrasound (LIPUS) on surgically managed fresh fractures. We therefore performed a multicenter retrospective cohort study to investigate the effects of LIPUS on surgically managed fresh fractures. METHODS: This study included patients surgically treated for diaphyseal fractures of the femur or tibia between August 2009 and July 2010 at 14 institutions. Outcome was the union period. We performed an overall comparison of the LIPUS group (78 cases) with the control group (63 cases), as well as subgroup analyses comparing outcomes for fracture sites, fracture types, soft tissue conditions, and fixation methods between the groups. RESULTS: There was no significant difference between the groups in terms of distribution of cases by fracture site, fracture type, soft tissue condition, fixation method. Analyses comparing subgroups, however, showed significant differences between the two groups, particularly in relation to type C fractures, regardless of whether all cases or only closed-fracture cases were analyzed: there was an approximately 30 % reduction in the union period for type C fractures in the LIPUS group. There were also cases requiring reoperation due to lack of stability, even among the type C fractures. CONCLUSIONS: LIPUS is effective for surgically managed, fresh, type C comminuted diaphyseal fractures of the lower limbs when there is appropriate stability at the fracture site.

DOI： 10.1007/s00776-013-0358-5

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