Faculty Profiles - OZAKI Toshifumi

Papers - OZAKI Toshifumi

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老年学的因子は浸潤性軟部肉腫患者の生存予後と相関するか

畑利彰, 藤原智洋, 柳井広之, 吉田晶, 板野拓人, 近藤彩奈, 片山晴喜, 佐藤浩平, 中田英二, 国定俊之, 尾崎敏文

日本整形外科学会雑誌 97 ( 2 ) S80 - S80 2023.3

がんロコモ診療の課題と展望周術期患者のがんロコモ・サルコペニア・フレイル

堅山佳美, 中田英二, 濱田全紀, 国定俊之, 藤原智洋, 千田益生, 尾崎敏文

日本整形外科学会雑誌 97 ( 2 ) S18 - S18 2023.3

小児AYA世代骨軟部腫瘍の課題と対策遺伝性骨・軟部腫瘍外来の取り組み

中田英二, 藤原智洋, 国定俊之, 尾崎敏文

中部日本整形外科災害外科学会雑誌 66 ( 春季学会 ) 58 - 58 2023.3

多診療科で考える脊椎転移の治療骨転移診療システムによる麻痺予防の取り組み

杉原進介, 近藤宏也, 中田英二, 尾崎敏文

日本整形外科学会雑誌 97 ( 3 ) S618 - S618 2023.3

脛骨遠位骨幹部骨折に対するsemi-extended approachは術後足関節アライメントを悪化させない

福岡史朗, 依光正則, 佐藤浩平, 上原健敬, 尾崎敏文, 寺田忠司

骨折 45 ( 2 ) 520 - 522 2023.3

思春期特発性側彎症(Lenke type 1)におけるHybrid法とSegmental Pedicle screw法による治療効果の検討

魚谷弘二, 植田昌敬, 志渡澤央和, 小田孔明, 三澤治夫, 尾崎敏文

中部日本整形外科災害外科学会雑誌 66 ( 春季学会 ) 329 - 329 2023.3

Malignant spinal cord compression(MSCC)を伴う転移性脊椎腫瘍患者に対する手術介入は生命予後に影響するか

志渡澤央和, 魚谷弘二, 三澤治夫, 鉄永倫子, 小田孔明, 植田昌敬, 尾崎敏文

日本整形外科学会雑誌 97 ( 2 ) S287 - S287 2023.3

転移性骨腫瘍キャンサーボードの運用と課題 Malignant spinal Cord Compression(MSCC)を伴う転移性脊椎腫瘍患者に対する手術介入と生命予後の関係

志渡澤央和, 魚谷弘二, 三澤治夫, 小田孔明, 植田昌敬, 尾崎敏文

中部日本整形外科災害外科学会雑誌 66 ( 春季学会 ) 106 - 106 2023.3

脛骨プラトー骨折と骨幹部骨折の合併骨折の手術的治療

福岡史朗, 依光正則, 佐藤浩平, 畑利彰, 上原健敬, 尾崎敏文

骨折 45 ( 2 ) 508 - 511 2023.3

解剖学的ランドマークを指標にした理想的なIAS挿入の位置と挿入角度の検討

福岡史朗, 依光正則, 佐藤浩平, 畑利彰, 上原健敬, 尾崎敏文

日本整形外科学会雑誌 97 ( 2 ) S244 - S244 2023.3

脛骨骨折に対する異なるアプローチ間での髄内釘の挿入位置の検討

福岡史朗, 依光正則, 上原健敬, 寺田忠司, 尾崎敏文

中部日本整形外科災害外科学会雑誌 66 ( 春季学会 ) 195 - 195 2023.3

人工肘関節置換術-合併症に対する創意と工夫- 人工肘関節周囲骨折の治療戦略

那須義久, 志水紀之, 浪花崇一, 堀田佳史, 原田遼三, 西田圭一郎, 尾崎敏文

日本整形外科学会雑誌 97 ( 3 ) S565 - S565 2023.3

日常生活中の筋電図記録に混入し得るノイズ信号に関する研究

皆木省吾, 兒玉直紀, 中原龍一, 尾崎敏文, 古寺寛志, 北川佳祐, 萬田陽介, 田中祐貴, 杉本皓, 森慧太朗, 山田蘭子, 松岸諒, 桑原実穂

日本顎口腔機能学会雑誌 29 ( 2 ) 124 - 125 2023.3

日常生活中の筋電図記録に混入し得るノイズ信号に関する研究

皆木省吾, 兒玉直紀, 中原龍一, 尾崎敏文, 古寺寛志, 北川佳祐, 萬田陽介, 田中祐貴, 杉本皓, 森慧太朗, 山田蘭子, 松岸諒, 桑原実穂

日本顎口腔機能学会雑誌 29 ( 2 ) 124 - 125 2023.3

A novel chondrocyte sheet fabrication using human-induced pluripotent stem cell-derived expandable limb-bud mesenchymal cells. International journal

Tomoka Takao, Masato Sato, Yuki Fujisawa, Eriko Toyoda, Daisuke Yamada, Yukio Hitsumoto, Eiji Nakata, Toshifumi Ozaki, Takeshi Takarada

Stem cell research & therapy 14 ( 1 ) 34 - 34 2023.2

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: Cell sheet fabrication for articular cartilage regenerative medicine necessitates a large number of chondrocytes of consistent quality as a cell source. Previously, we have developed human-induced pluripotent stem cell (iPSC)-derived expandable PRRX1+ limb-bud mesenchymal cells (ExpLBM) with stable expansion and high chondrogenic capacity, while in this study; our ExpLBM technology was combined with cell sheet engineering to assess its potential as a stable cell source for articular cartilage regeneration. METHODS: ExpLBM cells derived from human-induced pluripotent stem cells (hiPSCs), including 414C2 and Ff-KVs09 (HLA homozygous), were seeded onto a culture plate and two-dimensional chondrogenic induction (2-DCI) was initiated. After 2-DCI, ExpLBM-derived chondrocytes were stripped and transferred to temperature-responsive culture inserts and the chondrocyte sheets were histologically examined or transplanted into osteochondral knee defects of immunodeficient rats. RESULTS: Immunohistochemistry revealed that ExpLBM-derived cell sheets were positive for Safranin O, COL2, and ACAN but that they were negative for COL1 and RUNX2. Furthermore, the engrafted tissues in osteochondral knee defects in immunodeficient rats were stained with SafO, human VIMENTIN, ACAN, and COL2. CONCLUSIONS: The present study is the first to report the chondrocyte sheet fabrication with hiPSC-derived cell source. hiPSC-derived ExpLBM would be a promising cell source for cell sheet technology in articular cartilage regenerative medicine.

DOI： 10.1186/s13287-023-03252-4

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CDKAL1 Drives the Maintenance of Cancer Stem-Like Cells by Assembling the eIF4F Translation Initiation Complex. International journal

Rongsheng Huang, Takahiro Yamamoto, Eiji Nakata, Toshifumi Ozaki, Kazuhiko Kurozumi, Fanyan Wei, Kazuhito Tomizawa, Atsushi Fujimura

Advanced science (Weinheim, Baden-Wurttemberg, Germany) e2206542 2023.2

Language：English Publishing type：Research paper (scientific journal)

Cancer stem-like cells (CSCs) have a unique translation mode, but little is understood about the process of elongation, especially the contribution of tRNA modifications to the maintenance of CSCs properties. Here, it is reported that, contrary to the initial aim, a tRNA-modifying methylthiotransferase CDKAL1 promotes CSC-factor SALL2 synthesis by assembling the eIF4F translation initiation complex. CDKAL1 expression is upregulated in patients with worse prognoses and is essential for maintaining CSCs in rhabdomyosarcoma (RMS) and common cancers. Translatome analysis reveals that a group of mRNAs whose translation is CDKAL1-dependent contains cytosine-rich sequences in the 5' untranslated region (5'UTR). Mechanistically, CDKAL1 promotes the translation of such mRNAs by organizing the eIF4F translation initiation complex. This complex formation does not require the enzyme activity of CDKAL1 but requires only the NH2 -terminus domain of CDKAL1. Furthermore, sites in CDKAL1 essential for forming the eIF4F complex are identified and discovered candidate inhibitors of CDKAL1-dependent translation.

DOI： 10.1002/advs.202206542

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Association of phase angle with sarcopenia in chronic musculoskeletal pain patients: a retrospective study. International journal

Hironori Tsuji, Tomoko Tetsunaga, Haruo Misawa, Keiichiro Nishida, Toshifumi Ozaki

Journal of orthopaedic surgery and research 18 ( 1 ) 87 - 87 2023.2

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: In chronic musculoskeletal pain patients, detection of sarcopenia is of significant clinical interest. Phase angle, which can be measured through bioelectrical impedance analysis (BIA), can detect sarcopenia; however, the evidence in chronic musculoskeletal pain patients is limited. This study aimed to assess the relationship between phase angle and sarcopenia in patients with chronic musculoskeletal pain. Our hypothesis was that phase angle would be a useful indicator to identify sarcopenia in patients with chronic musculoskeletal pain. METHODS: A total of 190 patients (51 men and 139 women) with chronic musculoskeletal pain were included in this retrospective cross-sectional study. Patient data of backgrounds, numeric rating scale score for pain, skeletal muscle index, and phase angle assessed using BIA were retrospectively reviewed. Sarcopenia was diagnosed using the Asian Working Group for Sarcopenia criteria 2019. RESULTS: A total of 51 patients (26.7%), including 10 men (19.6%) and 41 women (29.5%), were diagnosed with sarcopenia. Phase angle, sarcopenia-related factors, age, and body mass index (BMI) differed significantly in patients with and without sarcopenia. On multiple logistic regression analysis, the prevalence of sarcopenia was significantly correlated with phase angle and BMI. The areas under the curve exhibited high accuracy in discriminating sarcopenia in men and moderate accuracy in both sexes and in women. CONCLUSIONS: Phase angle may be a valid discriminator of sarcopenia in patients with chronic musculoskeletal pain.

DOI： 10.1186/s13018-023-03567-1

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Expression of Acetabular Labral Vascular Endothelial Growth Factor and Nerve Growth Factor Is Directly Associated with Hip Osteoarthritis Pain: Investigation by Immunohistochemical Staining. International journal

Yoshihiro Sato, Tomonori Tetsunaga, Kazuki Yamada, Yoshi Kawamura, Aki Yoshida, Toshifumi Ozaki

International journal of molecular sciences 24 ( 3 ) 2023.2

Language：English Publishing type：Research paper (scientific journal)

The acetabular labrum enhances hip joint stability and plays a key role in osteoarthritis (OA) progression. Labral nerve endings contribute to hip OA pain. Moreover, vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) are associated with pain. Consequently, we analysed VEGF and NGF expression levels in the labrum and their roles in OA. Labra obtained from OA patients were stained immunohistochemically, and labral cells were cultured and subjected to a reverse transcription (RT)-polymerase chain reaction (PCR) to analyse VEGF and NGF mRNA expression. VEGF and NGF expression were compared in each region of the labrum. Correlations between VEGF and NGF expression and age, body mass index, Kellgren-Lawrence grade, Harris Hip Score, the visual analogue scale (VAS), and Krenn score were analysed, and the RT-PCR confirmed the findings. VEGF and NGF expression were high on the labral articular side, negatively correlated with the Krenn score, and positively correlated with the VAS in early OA. VEGF and NGF mRNA expression increased significantly in patients with severe pain and decreased significantly in severely degenerated labra. In early OA, VEGF and NGF expression in the acetabular labrum was associated with the occurrence of hip pain; therefore, these factors could be effective targets for pain management.

DOI： 10.3390/ijms24032926

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悪性末梢神経鞘腫瘍におけるPRRX1の悪性化因子としての役割

たき平将太, 中田英二, 山田大祐, 片山晴喜, 畑利彰, 藤原智洋, 高尾知佳, 国定俊之, 宝田剛志, 尾崎敏文

日本レックリングハウゼン病学会学術大会プログラム・抄録集 14回 16 - 16 2023.2

神経線維腫症1型における院内レジストリの構築と定期的なサーベイランスの実施

二川摩周, 中田英二, 十川麗美, 加藤芙美乃, 浦川優作, 山本英喜, 藤原智洋, 国定俊之, 平沢晃, 尾崎敏文

日本レックリングハウゼン病学会学術大会プログラム・抄録集 14回 34 - 34 2023.2

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