Faculty Profiles - KUBOKI Takuo

MISC - KUBOKI Takuo

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【下顎臼歯部少数歯欠損の処置方針】歯科医療の不確実性とEvidence-based Medicine(EBM)

窪木拓男

日本歯科評論 ( 709 ) 91 - 96 2001.11

Signal Intensity Changes in T2-weighted Magnetic Resonance Imaging in Trapezius Muscle to Cold Pressor Stimulation

MINAKUCHI M, MAEKAWA K, YANAGI Y, INOUE E, SUZUKI K, KUBOKI T, YATANI H, KISHI K, CLARK GT

45 ( 106 ) 182 - 182 2001.10

21世紀の咬合学根拠を求めて臨床エビデンスと分子生物学的視点からみた顎関節症

窪木拓男

日本矯正歯科学会大会プログラム・抄録集 60回 103 - 103 2001.10

エビデンスを「創る」治療効果を明らかにするための研究デザイン

窪木拓男

日本補綴歯科学会雑誌 45 ( 106回特別 ) 47 - 47 2001.10

修復象牙質形成促進時における結合組織成長因子の局在とその発現制御機構

園山亘, 窪木拓男, 江口傑徳, 小森千尋, 藤沢拓生, 完山学, 矢谷博文

日本補綴歯科学会雑誌 45 ( 106回特別 ) 135 - 135 2001.10

関節軟骨細胞の増殖・基質合成・石灰化に対する結合組織成長因子の影響

小森千尋, 窪木拓男, 水島恒尚, 園山亘, 藤澤拓生, 完山学, 矢谷博文

日本補綴歯科学会雑誌 45 ( 106回特別 ) 181 - 181 2001.10

Promoter activity determinant of human connective tissue growth factor (CTGF/Hcs24) gene in a human chondrocytic cell line, HCS-2/8.

T Eguchi, S Kubota, S Kondo, T Shimo, T Nakanishi, T Kuboki, H Yatani, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 16 S326 - S326 2001.9

顔面外傷後の顎顔面変形とこう合異常を呈した症例

藤山光治, 山城隆, 宮脇正一, 山本照子, 香川智正, 三島克章, 菅原利夫, 窪木拓男, 矢谷博文

日本顎変形症学会雑誌 11 ( 2 ) 163 - 163 2001.8

ヒト軟骨様細胞株HCS-2/8におけるCTGF/Hcs24遺伝子のプロモーター活性決定因子

江口傑徳, 久保田聡, 近藤誠二, 志茂剛, 中西徹, 窪木拓男, 矢谷博文, 滝川正春

歯科基礎医学会雑誌 43 ( 5 ) 557 - 557 2001.8

マウス下顎頭軟骨におけるCbfa1遺伝子の発現

秋山謙太郎, 窪木拓男, 完山学, 縄稚久美子, 矢谷博文, 山下和夫, 山本照子, 中西徹, 滝川正春

歯科基礎医学会雑誌 43 ( 5 ) 583 - 583 2001.8

Regulatory mechanism of human connective tissue growth factor (CTGF/Hcs24) gene expression in a human chondrocytic cell line, HCS-2/8

T Eguchi, S Kubota, S Kondo, T Shimo, T Hattori, T Nakanishi, T Kuboki, H Yatani, M Takigawa

JOURNAL OF BIOCHEMISTRY 130 ( 1 ) 79 - 87 2001.7

Language：English Publisher：OXFORD UNIV PRESS

CTGF/Hcs24 is a multi-functional growth factor that potentiates either the growth or differentiation of mesenchymal cells, according to the biological conditions. Among various functional aspects of CTGF/Hcs24, it is especially notable that CTGF/Hcs24 may promote endochondral ossification in growth cartilage through all stages, and it is highly expressed in a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8). In this study, to clarify the regulatory mechanism of CTGF/Hcs24 gene expression in chondrocytes, we analyzed the transcriptional activity of the CTGF/Hcs24 promoter and the effect of the CTGF/Hcs24 S'-untranslated region (3'-UTR) on gene expression in HCS-2/8 by means of an established DNA transfection and luciferase reporter gene assay system. As a result, the luciferase activity of the CTGF/Hcs24 promoter was found to be remarkably high in HCS-2/8. The 3'-UTR of the CTGF/Hcs24 gene strongly repressed the luciferase activity in HCS-2/8, when it was linked to the downstream of the luciferase reporter gene, suggesting its functionality also in chondrocytic cells. Deletion analysis of the CTGF/Hcs24 promoter clarified a major segment responsible for the enhanced CTGF/Hcs24 promoter activity in HCS-2/8, The TGF-beta response element in the DNA segment was active in HCS-2/8, and point mutations in the element moderately decreased the highly maintained promoter activity with total loss of TGF-beta responsiveness. These results indicate that the strong expression of the CTGF/Hcs24 gene in HCS-2/8 was mainly caused by high transcriptional activity of the CTGF/Hcs24 promoter, and that the TGF-beta response element is one of the critical elements that support the high transcription activity.

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Intramuscular haemodynamic responses to different durations of sustained extension in normal human masseter

M Inoue-Minakuchi, K Maekawa, T Kuboki, K Suzuki, A Yamashita, H Yatani, T Clark

ARCHIVES OF ORAL BIOLOGY 46 ( 7 ) 661 - 666 2001.7

Language：English Publisher：PERGAMON-ELSEVIER SCIENCE LTD

Ten healthy non-smoking males (mean age 24.3 +/- 0.8 years) with no history of chronic muscle pain or migraine participated in this study. Intramuscular total haemoglobin (Hb), an indicator of blood volume in the illuminated area, was measured with a non-invasive, near-infrared spectroscopic device. Each participant was told to maintain maximal mouth opening to extend the masseter muscle for 30, 60 or 120 s in random order. Data were continuously recorded from the right masseter 1 min before, at set times during and for 5 min after sustained muscle extension in each trial. Each trial was separated by a 10-min interval. Heart rate (HR) and blued pressure (BP) were also recorded. The mean normalized Hb decreased during muscle extension and rebound hyperaemia was observed after it in each trial (P = 0.0001). Hb returned to baseline within 60 s. The magnitude of the decremental change Juring extension and of the incremental change in the rebound hyperaemia was not significantly different among the three trials (P = 0.9071); neither were mean normalized HR and BP. These data suggest that sustained extension of the masseter produces a reduction in total intramuscular Hb during extension and a secondary increase in Hb following a return to the resting muscle's normal length. (C) 2001 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S0003-9969(00)00138-2

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ヒト軟骨肉腫由来軟骨様細胞株HCS-2/8における結合組織成長因子CTGF/Hcs24遺伝子のプロモーター活性決定因子

江口傑徳, 久保田聡, 近藤誠二, 志茂剛, 中西徹, 窪木拓男, 矢谷博文, 滝川正春

日本骨代謝学会雑誌 19 ( 2 ) 102 - 102 2001.7