Faculty Profiles - KUBOKI Takuo

MISC - KUBOKI Takuo

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Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF-β3-Associated Chondrogenesis of Bone Marrow-Derived Mesenchymal Stem Cells for Articular Surface Regeneration. International journal

Emilio Satoshi Hara, Mitsuaki Ono, Hai Thanh Pham, Wataru Sonoyama, Satoshi Kubota, Masaharu Takigawa, Takuya Matsumoto, Marian F Young, Bjorn R Olsen, Takuo Kuboki

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 30 ( 9 ) 1585 - 96 2015.9

Language：English Publisher：WILEY

Articular cartilage repair remains a challenging problem. Based on a high-throughput screening and functional analysis, we found that fluocinolone acetonide (FA) in combination with transforming growth factor beta 3 (TGF-β3) strongly potentiated chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). In an in vivo cartilage defect model in knee joints of immunocompromised mice, transplantation of FA/TGF-β3-treated hBMSCs could completely repair the articular surface. Analysis of the intracellular pathways revealed that FA enhanced TGF-β3-induced phosphorylation of Smad2 and Smad3. Additionally, we performed a pathway array and found that FA activates the mTORC1/AKT pathway. Chemical inhibition of mTORC1 with rapamycin substantially suppressed FA effect, and inhibition of AKT completely repressed chondrogenesis of hBMSCs. Inhibition of glucocorticoid receptor with mifepristone also suppressed FA effect, suggesting that FA involves binding to the glucocorticoid receptor. Comparative analysis with other glucocorticoids (triamcinolone acetonide [TA] and dexamethasone [DEX]) revealed the unique ability of FA to repair articular cartilage surgical defects. Analysis of intracellular pathways showed that the mTORC1/AKT pathway and the glucocorticoid receptor was highly activated with FA and TA, but to a lesser extent with DEX. Collectively, these results show a unique ability of FA to enhance TGF-β3-associated chondrogenesis, and suggest that the FA/TGF-β3 combination may be used as major inducer of chondrogenesis in vitro. Additionally, FA/TGF-β3 could be potentially applied in a clinical setting to increase the efficiency of regenerative approaches based on chondrogenic differentiation of stem cells.

DOI： 10.1002/jbmr.2502

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PubMed

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Effect of prosthetic restoration on oral health-related quality of life in patients with shortened dental arches: a multicentre study

K. Fueki, Y. Igarashi, Y. Maeda, K. Baba, K. Koyano, K. Sasaki, Y. Akagawa, T. Kuboki, S. Kasugai, N. R. Garrett

JOURNAL OF ORAL REHABILITATION 42 ( 9 ) 701 - 708 2015.9

Language：English Publisher：WILEY-BLACKWELL

The aim of this multicentre prospective study was to investigate the effect of prosthetic restoration for missing posterior teeth in patients with shortened dental arches (SDAs). SDA patients with 2-12 missing occlusal units (a pair of occluding premolars corresponds to one unit, and a pair of occluding molars corresponds to two units) were consecutively recruited from seven university-based dental hospitals in Japan. Patients chose no replacement of missing teeth or prosthetic treatment with removable partial dentures (RPDs) or implant-supported fixed partial dentures (IFPDs). Oral health-related quality of life (OHRQoL) was measured using the oral health impact profile (Japanese version - OHIP-J) at baseline and follow-up/post-treatment evaluation. Of the 169 subjects who completed baseline evaluation, 125 subjects (mean age; 63.0 years) received follow-up/post-treatment evaluation. No-treatment was chosen by 42% (53/125) of the subjects, and 58% (72/125) chose treatment with a RPD (n = 53) or an IFPD (n = 19). In the no-treatment (NT) group, the mean OHIP summary score at baseline was similar to that at follow-up evaluation (P = 0.69). In the treatment (TRT) group, the mean OHIP summary score decreased significantly after the RPD treatment (P = 0.002), and it tended to decrease, though not statistically significant (P = 0.18), after the IFPD treatment. The restoration of one occlusal unit was associated with a 1.2-point decrease in OHIP summary score (P = 0.034). These results suggest that the replacement of missing posterior teeth with RPDs or IFPDs improved OHRQoL. Prosthetic restoration for SDAs may benefit OHRQoL in patients needing replacement of missing posterior teeth.

DOI： 10.1111/joor.12297

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【プラスティックサージェリーはインプラント治療の何を変えるのか?】審美領域における口腔インプラント治療の予後の安定性

窪木拓男

Quintessence DENTAL Implantology 22 ( 5 ) 688 - 691 2015.9

CCN4/WISP-1はTGF-b3誘導性軟骨細胞分化を正に制御する

吉岡裕也, 大野充昭, 窪木拓男

Journal of Oral Biosciences Supplement 2015 265 - 265 2015.9

要支援・要介護高齢者における低体重発生のリスク因子の検討

小山絵理, 大野彩, 山本道代, 三野卓哉, 黒崎陽子, 中川晋輔, 瀧内博也, 縄稚久美子, 水口真実, 水口一, 前川賢治, 窪木拓男

老年歯科医学 30 ( 2 ) 105 - 106 2015.9

要介護高齢者の口腔健康および摂食と主たる介護者の介護負担感との関連

山本道代, 大野彩, 小山絵理, 三野卓哉, 黒崎陽子, 中川晋輔, 瀧内博也, 水口真実, 水口一, 縄稚久美子, 前川賢治, 窪木拓男

老年歯科医学 30 ( 2 ) 135 - 135 2015.9

全身機能が低下した高齢インプラント患者の口腔状態および歯科介入環境に関する報告

黒崎陽子, 大野彩, 三野卓哉, 沼本賢, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 294 - 294 2015.8

口腔内写真を効率良く運用・管理するデジタルワークフロー

窪田努, 林宏規, 荒川光, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 321 - 321 2015.8

カスタム印象用コーピングを用いたインプラント補綴の一例

高橋康治, 荒川光, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 192 - 192 2015.8

即時荷重インプラントを用いて全顎的な咬合再構成を行った1症例

吉見哲朗, 荒川光, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 210 - 210 2015.8

上皮下結合組織移植術の併用により上顎前歯部に口腔インプラント治療を行った1症例

小田師巳, 稲野眞治, 荒川光, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 223 - 223 2015.8

超高齢社会における口腔インプラント学会専門医の責務とは最期まで患者に寄り添うために

窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 80 - 80 2015.8

BMP2変異体L51PはBMPネガティブ・フィードバック機構を制御することでBMP2誘導性骨芽細胞分化および骨形成を促進する

大野充昭, Khattb Hany, 笈田育尚, Sebald Walter, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 178 - 178 2015.8

天然歯との調和をはかった前歯部インプラント治療一症例

石井ちひろ, 荒川光, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 191 - 191 2015.8

上顎洞粘液嚢胞を穿刺吸引し上顎洞底挙上術とインプラント体埋入手術を同時に行った1症例

南口真実, 高山賢一, 近藤充茂, 窪木拓男, 桐田忠昭

日本口腔インプラント学会誌 28 ( 特別号 ) 224 - 224 2015.8

移植材を使用しない歯槽頂アプローチによる上顎洞底挙上術 10ケースの症例報告

堀良彦, 荒川光, 小田師巳, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 231 - 231 2015.8

口腔インプラント患者におけるドロップアウト関連要因の調査

藤原和栄, 荒川光, 田中力, 窪木拓男

日本口腔インプラント学会誌 28 ( 特別号 ) 268 - 268 2015.8

A longitudinal retrospective study of the analysis of the risk factors of implant failure by the application of generalized estimating equations.

Kinji Noda, Hikaru Arakawa, Aya Kimura-Ono, Seiya Yamazaki, Emilio Satoshi Hara, Wataru Sonoyama, Kenji Maekawa, Kazuo Okura, Ayumi Shintani, Yoshizo Matsuka, Takuo Kuboki

Journal of prosthodontic research 59 ( 3 ) 178 - 84 2015.7

Language：English Publisher：ELSEVIER IRELAND LTD

PURPOSE: Many studies have identified risk factors for dental implant failure, although few have investigated the correlation among implant fixtures within single patients. A better analytical method may include repeated measures analysis including generalized estimating equations (GEE). This retrospective cohort study aimed to (1) identify the risk factors for failure of dental implantation and (2) evaluate an analytical method using GEE analysis. METHODS: We analyzed data on early and late implant failures in 296 patients providing 721 rough surface dental implants (2.44 implants per patient). Potential predictors of implant failure included age, gender, smoking, location of implant, use of bone augmentation, number of remaining teeth, opposing tooth condition, fixture length, fixture diameter and type of suprastructure (fixed or removable partial denture). The likelihood of early and late implant failure was estimated by GEE. RESULTS: The early failure rate was 1.5% (11/721 implants, 7/296 patients) and the 10-year cumulative survival rate was 94.0% (7/710 implants, 5/293 patients). The GEE analysis revealed that a significant risk factor for early implant failure was smoking (p<0.01), whereas significant risk factors for late failure were maxillary implant (p=0.02), posterior implant (p<0.01), number of remaining teeth (≥20) (p<0.01), opposing unit being a removable partial denture or nothing (p=0.04) and having a removable type suprastructure (p<0.01). CONCLUSIONS: GEE analysis showed that smoking was a risk factor for early implant failure, and several risk factors were identified for late implant failure.

DOI： 10.1016/j.jpor.2015.04.003

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PubMed

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顎関節症と類似した症状を呈し鑑別診断に苦慮した頭頸部腫瘍の一例

三木春奈, 水口一, 前川賢治, 窪木拓男

日本顎関節学会雑誌 27 ( Suppl. ) 171 - 171 2015.7

岡山大学における臨床講師を利用した在宅介護・訪問歯科診療参加型学外臨床実習について

杉本恭子, 宮脇卓也, 武田宏明, 前田茂, 曽我賢彦, 森田学, 鳥井康弘, 窪木拓男

日本歯科医学教育学会総会・学術大会プログラム・抄録集 34回 79 - 79 2015.7

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