Faculty Profiles - KUBOKI Takuo

Selective electrical Current Perception Threshokds ing the Trigeminal and Medianus Nerves Estimation lf the sex difference of CPT using the Meurometer【○!R】

軟骨細胞におけるチロシンキナーゼ型レセプターErbB4遺伝子の発現

縄稚久美子, 久保田聡, 井上美穂, 西田崇, 吉道玄, 中西徹, 完山学, 窪木拓男, 矢谷博文, 山合友一郎, 滝川正春

生化学 74 ( 11 ) 1413 - 1413 2002.11

NAKAZAWA Y, MINAKUCHI H, MINAKUCHI H, YATAMI H

46 ( 108 ) 143 - 143 2002.10

How to Utilize Clinical Evidence

KUBOKI Takuo

46 ( 108 ) 46 - 48 2002.10

Hypothakamic-Pitutary-Axis Hornones and Painful Cold Pressor Stimulation

SUZUKI K, MAEKAWA K, NAKAZAWA Y, KUBOKI T, YATANI H

46 ( 108 ) 175 - 175 2002.10

片側臼歯部交叉咬合患者の顎関節空隙について

片山朗, 宮脇正一, 谷本裕子, 荒木佳子, 窪木拓男, 山本照子

日本矯正歯科学会大会プログラム・抄録集 61回 232 - 232 2002.10

Tyrosine kinase-type receptor ErbB4 in chondrocytes: interaction with connective tissue growth factor and distribution in cartilage. International journal

Kumiko Nawachi, Miho Inoue, Satoshi Kubota, Takashi Nishida, Gen Yosimichi, Tohru Nakanishi, Manabu Kanyama, Takuo Kuboki, Hirofumi Yatani, Tomoichiro Yamaai, Masaharu Takigawa

FEBS letters 528 ( 1-3 ) 109 - 13 2002.9

Language：English Publisher：ELSEVIER SCIENCE BV

In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24.

DOI： 10.1016/S0014-5793(02)03263-5

Effects of IL-1beta and LPS on CTGF expression in mouse-derived odontoblast-like cells, MDPC-23.

W Sonoyama, T Kuboki, T Fujisawa, T Eguchi, J Uehara, H Yatani, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 17 S327 - S327 2002.9

CTGF/Hcs24, a hypertrophic chondrocyte-specific gene product, stimulates proliferation and differentiation but not hypertrophy of cultured articular chondrocytes. International journal

T Nishida, S Kubota, T Nakanishi, T Kuboki, G Yosimichi, S Kondo, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 17 ( 1 ) S180 - S181 2002.9

Language：English Publisher：AMER SOC BONE & MINERAL RES

We previously reported that connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) stimulated the proliferation and differentiation of rabbit growth cartilage (RGC) cells in vitro. In this study, we investigated the effects of CTGF/Hcs24 on the proliferation and differentiation of rabbit articular cartilage (RAC) cells in vitro. RAC cells transduced by recombinant adenoviruses generating mRNA for CTGF/Hcs24 synthesized more proteoglycan than the control cells. Also, treatment of RAC cells with recombinant CTGF/Hcs24 (rCTGF/Hcs24) increased DNA and proteoglycan syntheses in a dose-dependent manner. Northern blot analysis revealed that the rCTGF/Hcs24 stimulated the gene expression of type II collagen and aggrecan core protein, which are markers of chondrocyte maturation, in both RGC and RAC cells. However, the gene expression of type X collagen, a marker of hypertrophic chondrocytes, was stimulated by rCTGF/Hcs24 only in RGC cells, but not in RAC cells. Oppositely, gene expression of tenascin-C, a marker of articular chondrocytes, was stimulated by rCTGF/Hcs24 in RAC cells, but not in RGC cells. Moreover, rCTGF/Hcs24 effectively increased both alkaline phosphatase (ALPase) activity and matrix calcification of RGC cells, but not of RAC cells. These results indicate that CTGF/Hcs24 promotes the proliferation and differentiation of articular chondrocytes, but does not promote their hypertrophy or calcification. Taken together, the data show that CTGF/Hcs24 is a direct growth and differentiation factor for articular cartilage, and suggest that it may be useful for the repair of articular cartilage.

チロシンキナーゼ型レセプターErbB4遺伝子の軟骨細胞における発現

Novel cis-element TRENDIC that enhance connective tissue growth factor (ctgf) gene expression in chondrocytic HCS-2/8.

T Eguchi, S Kubota, S Kondo, Y Mukudai, T Kuboki, H Yatani, M Takigawa

JOURNAL OF BONE AND MINERAL RESEARCH 17 S224 - S224 2002.9

縄稚久美子, 久保田聡, 西田崇, 吉道玄, 中西徹, 完山学, 窪木拓男, 矢谷博文, 山合友一郎, 滝川正春, 井上美穂

歯科基礎医学会雑誌 44 ( 5 ) 388 - 388 2002.9

Quality of life assessment in patients with implant-supported and resin-bonded fixed prosthesis for bounded edentulous spaces. International journal

Wataru Sonoyama, Takuo Kuboki, Soichiro Okamoto, Hidenori Suzuki, Hikaru Arakawa, Manabu Kanyama, Hirofumi Yatani, Atsushi Yamashita

Clinical oral implants research 13 ( 4 ) 359 - 64 2002.8

Language：English Publisher：BLACKWELL MUNKSGAARD

OBJECTIVES: Dental implants have become increasingly popular in the prosthetic rehabilitation of patients with bounded edentulous spaces. Oral condition-related quality of life (QOL) levels have rarely been assessed in these patients. MATERIAL AND METHODS: Two groups of subjects with bounded edentulous spaces were studied: an implant-supported fixed prosthesis group (11 patients) and a resin-bonded fixed prosthesis group (33 patients). The two groups were well matched in terms of sex, age, missing units and location of missing units. The patients were requested to answer a self-administered QOL questionnaire with two major subscales - oral condition- and general condition-related QOL scores. The test-retest reliability of each question was pre-examined and found acceptable (mean Spearman rank correlation coefficient was 0.55 +/- 0.16). Mean QOL score differences between the two groups were analyzed by the Mann-Whitney U-test. RESULTS: Mean oral condition-related QOL scores of the implant-supported and resin-bonded fixed prosthesis groups were 87.8 +/- 9.5 and 87.1 +/- 12.3% (P = 0.85), and mean general condition-related QOL scores were 73.8 +/- 14.8 and 71.6 +/- 15.2% (P = 0.95), respectively. No significant QOL differences between the two groups were observed in the two subscales. CONCLUSION: In patients with bounded edentulous spaces, multidimensional QOL levels of patients with an implant-supported fixed prosthesis do not exceed those of patients with a resin-bonded fixed prosthesis in a short follow-up period.

DOI： 10.1034/j.1600-0501.2002.130403.x

Reply to commentaries

GT Clark, K Maekawa, T Kuboki

JOURNAL OF PAIN 3 ( 4 ) 270 - 271 2002.8

Intramuscular hypoperfusion, adrenergic receptors, and chronic muscle pain. International journal

Kenji Maekawa, Glenn T Clark, Takuo Kuboki

The journal of pain : official journal of the American Pain Society 3 ( 4 ) 251 - 60 2002.8

Language：English Publisher：CHURCHILL LIVINGSTONE

Despite a high prevalence of chronic muscle pain disorders such as fibromyalgia and regional myofascial pain, there is still limited knowledge about the factors that initiate and perpetuate these pain states. Although there are also likely to be downstream neuropathic changes in the central nervous system and spinal cord that sustain and exacerbate the pain states known as fibromyalgia, the focus of this critical review is on studies that examined the connection between both fibromyalgia and regional myofascial pain and sympathetic function. Specifically, we looked at studies that described Raynaud-like symptoms, cardiovascular dysfunction and altered intramuscular perfusion in chronic muscle pain. Our analysis showed that although the first 2 phenomena were intermittently present, a prominent and consistent feature for regional myofascial pain and to a lesser degree for fibromyalgia was intramuscular hypoperfusion. Several hypotheses can be offered why this hypoperfusion exists, and additional studies comparing and contrasting these theories are needed. This review focuses on one of these theories, namely, agonist-induced beta-adrenergic receptor desensitization as an explanatory model for hypoperfusion. What cannot be done at this time and is needed in the future is to compare and contrast to what degree the regional muscle pain disorder (myofascial) is similar or different from the more generalized disorder (fibromyalgia).

DOI： 10.1054/jpai.2002.125923

A novel cis-element that enhances connective tissue growth factor gene expression in chondrocytic cells. International journal

Takanori Eguchi, Satoshi Kubota, Seiji Kondo, Takuo Kuboki, Hirofumi Yatani, Masaharu Takigawa

Biochemical and biophysical research communications 295 ( 2 ) 445 - 51 2002.7

Language：English Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE

To clarify the chondrocyte-specific regulatory mechanism of connective tissue growth factor (ctgf) gene expression, we analyzed the functionality and DNA-protein interaction of the CTGF promoter. Comparative luciferase assay of the CTGF promoter deletion mutants among HCS-2/8 chondrocytic cells and fibroblastic cells revealed that a 110-bp region in the promoter was crucial for the HCS-2/8-specific transcriptional enhancement. Subsequent competitive gel shift assay revealed that transcription factors in HCS-2/8 nuclei bound to a 60-bp portion in the corresponding region. Relative luciferase activity from a CTGF promoter with mutant TGF-beta response element (TbRE) was 16.9% lower than that from an intact promoter. On the other hand, relative luciferase activity from a CTGF promoter with 4bp point mutations at 30bp upstream of the TbRE was 47.7% lower than that from the intact one. The binding activity of HCS-2/8 nuclear factor(s) to the sequence over the 4-bp was remarkably higher than that of any nuclear extract from other types of cells. Therefore, we entitled the sequence 'TRENDIC', a transcription enhancer dominant in chondrocytes, which stands for a novel enhancer for chondrocyte-specific CTGF gene expression.

DOI： 10.1016/S0006-291X(02)00700-3

Reply to commentaries

J de Peyer

PSYCHOANALYTIC DIALOGUES 12 ( 4 ) 545 - 549 2002.7

CTGF/Hcs24, a hypertrophic chondrocyte-specific gene product, stimulates proliferation and differentiation, but not hypertrophy of cultured articular chondrocytes. International journal

Takashi Nishida, Satoshi Kubota, Tohru Nakanishi, Takuo Kuboki, Gen Yosimichi, Seiji Kondo, Masaharu Takigawa

Journal of cellular physiology 192 ( 1 ) 55 - 63 2002.7

Language：English Publisher：WILEY-LISS

DOI： 10.1002/jcp.10113

顎口腔領域の各種感覚刺激によるMRI脳機能画像分析

岸幹二, 若狭亨, 繁原宏, 窪木拓男, 杉本朋貞

INNERVISION 17 ( 8 ) 52 - 52 2002.7

Language：Japanese Publisher：(株)インナービジョン

顎口腔領域における各種感覚刺激のfMRIに最適の撮像条件の設定,頭部の固定法,画像処理を確立し,味覚刺激及び嚥下運動に関しfMRIの描出能を検討し,fMRIの顎口腔領域各種感覚刺激の研究,臨床応用の可能性を追及した.その結果,味覚刺激及び嚥下運動による脳賦活部位がfMRIによって検出することが可能であった