Faculty Profiles - KUBOKI Takuo

The Quasi-Three-Dimensional Marginal Leakage of Full-Coverage Crowns: Resin Coating Versus Sodium Hypochlorite Treatment

Daizo Kawahara, Atsushi Mine, Jan De Munck, Takuo Kuboki, Yasuhiro Yoshida, Kazuomi Suzuki, Bart Van Meerbeek, Hirofumi Yatani

INTERNATIONAL JOURNAL OF PROSTHODONTICS 23 ( 5 ) 406 - 409 2010.9

Language：English Publisher：QUINTESSENCE PUBLISHING CO INC

This study compared the effects of various surface treatments and techniques on the marginal leakage of full-coverage crowns using a quasi-three-dimensional evaluation. Crowns were cast using a gold-silver-palladium alloy by means of the lost-wax technique. Twenty-eight recently extracted human molars were divided randomly into four groups according to surface treatment before crown cementation: (1) no pretreatment (negative control), (2) primer (positive control), (3) resin coating and primer, and (4) phosphoric acid, sodium hypochlorite, and primer. All specimens were cemented with composite cement. The lowest marginal leakage was observed in group 4. Variation in marginal leakage between specimens originating from the same tooth was observed. Int J Prosthodont 2010; 23: 406-409.

欠損補綴のパラダイムシフトと口腔インプラント口腔インプラントで患者の満足を勝ち取るには

窪木拓男

日本口腔インプラント学会誌 23 ( 3 ) 500 - 501 2010.9

ヘパリン結合活性を増強した大腸菌由来改良型BMP-2を用いた骨形成能を有する口腔インプラントの開発

笈田育尚, 下野賢吾, 大野充昭, 完山学, 窪木拓男

日本口腔インプラント学会誌 23 ( 特別号 ) 150 - 150 2010.8

Relationship between a chronically painful trapezius muscle and its metabolic state analyzed with PET/CT. International journal

Satoru Takiguchi, Kenji Maekawa, Tsuyoshi Ono, Nobuya Sasai, Mitsumasa Kaji, Glenn T Clark, Takuo Kuboki

Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 110 ( 1 ) 54 - 61 2010.7

Language：English Publisher：MOSBY-ELSEVIER

OBJECTIVES: The aim of this study was to investigate the intramuscular metabolic state in chronically painful muscles using positron-emission tomography/computerized tomography (PET/CT). STUDY DESIGN: The study included 140 consecutive noncancer subjects who underwent PET/CT screening for a physical checkup (mean age 56.0 +/- 10.22 y). The demographic data and information on pain in the neck/shoulder region were obtained using a questionnaire. The subjects who had an awareness of pain in the neck/shoulder region for >6 months were regarded to be pain subjects (n = 39). The minimal and maximal standardized uptake values (SUV) of [(18)F]fluorodeoxyglucose ((18)F-FDG) of the trapezius muscle in each subject were automatically calculated. RESULTS: The unpaired t test revealed that both the minimal and the maximal SUVs were significantly lower in the pain subjects than in the asymptomatic subjects. A multiple linear regression analysis also demonstrated a significant association between pain in the neck/shoulder region and the SUVs in the trapezius muscle. CONCLUSIONS: Uptake of (18)F-FDG was lower in the chronically painful trapezius muscle.

DOI： 10.1016/j.tripleo.2010.02.028

WISP-1/CCN4はBMP-2の機能を制御することで骨形成を促進する

大野充昭, 園山亘, 窪木拓男, ヤング・マリアン

日本骨代謝学会学術集会プログラム抄録集 28回 163 - 163 2010.7

電子痛覚測定装置による歯根膜感覚の客観的評価の検討

平井真也, 小川匠, 松香芳三, 重田優子, 安藤栄里子, 平林里大, 井川知子, 笠間慎太郎, 窪木拓男, 福島俊士, クラーク・グレン

日本顎関節学会雑誌 22 ( Suppl. ) 93 - 93 2010.7

TEM characterization of a silorane composite bonded to enamel/dentin. International journal

Atsushi Mine, Jan De Munck, Annelies Van Ende, Marcio Vivan Cardoso, Takuo Kuboki, Yasuhiro Yoshida, Bart Van Meerbeek

Dental materials : official publication of the Academy of Dental Materials 26 ( 6 ) 524 - 32 2010.6

Language：English Publisher：ELSEVIER SCI LTD

OBJECTIVES: The low-shrinking composite composed of combined siloxane-oxirane technology (Filtek Silorane, 3M ESPE, Seefeld, Germany) required the development of a specific adhesive (Silorane System Adhesive, 3M ESPE), in particular because of the high hydrophobicity of the silorane composite. The purpose of this study was to characterize the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). METHODS: Non-demineralized/demineralized 70-90 nm sections were prepared following common TEM specimen processing procedures. RESULTS: TEM revealed a typical twofold build-up of the adhesive resin, resulting in a total adhesive layer thickness of 10-20 microm. At bur-cut enamel, a tight interface without distinct dissolution of hydroxyapatite was observed. At bur-cut dentin, a relatively thin hybrid layer of maximum a few hundreds of nanometer was formed without clear surface demineralization. No clear resin tags were formed. At fractured dentin, the interaction appeared very superficial (100-200 nm). Distinct resin tags were formed due to the absence of smear plugs. Silver-nitrate infiltration showed a varying pattern of both spot- and cluster-like appearance of nano-leakage. Traces of Ag were typically detected along some part of the enamel-adhesive interface and/or between the two adhesive resin layers. Substantially more Ag-infiltration was observed along the dentin-adhesive interface of bur-cut dentin, as compared to that of fractured dentin. CONCLUSIONS: The nano-interaction of Silorane System Adhesive should be attributed to its relatively high pH of 2.7. The obtained tight interface at both enamel and dentin indicates that the two-step self-etch adhesive effectively bridged the hydrophilic tooth substrate with the hydrophobic silorane composite.

DOI： 10.1016/j.dental.2010.01.010

研究室から診療室へ State of the art,Prosthodontics 臨床家が知っておきたい補綴最新トピックス(第4回) 審美歯科、歯周外科処置と補綴治療の融合

窪木拓男, 金田貴哲, 佐藤毅

補綴臨床 43 ( 3 ) 343 - 353 2010.5

周術期における歯科介入の重要性岡山大学病院周術期管理センター歯科部門の取り組み

丸山貴之, 山中玲子, 曽我賢彦, 縄稚久美子, 柳文修, 兒玉直紀, 中田貴, 村田尚道, 有岡享子, 山根美榮子, 三浦留美, 羽川操, 木南美香, 高橋明子, 住吉由季子, 竹内哲男, 窪木拓男, 佐々木朗, 森田学

日本口腔ケア学会雑誌 4 ( 1 ) 83 - 83 2010.3

The effect of growth factors for bone augmentation to enable dental implant placement: A systematic review

Kengo Shimono, Masamitsu Oshima, Hikaru Arakawa, Aya Kimura, Kumiko Nawachi, Takuo Kuboki

Japanese Dental Science Review 46 ( 1 ) 43 - 53 2010.2

Language：English Publishing type：Book review, literature introduction, etc.

This systematic review assessed the potential benefits of growth factors for bone augmentation prior to the placement of dental implants in human. A systematic online review of the Medline database, using the PubMed search machine was performed between 1966 and November 2008 by entering the MeSH terms. The primary outcome of the included studies was bone regeneration of localized alveolar ridge defects. The initial search identified 119 papers from the electronic database. This review produced seven eligible papers that reported on bone augmentation with recombinant human Bone Morphogenetic Protein-2 (rhBMP-2), recombinant human Platelet-Derived Growth Factor (rhPDGF) and Plasma-Rich Growth Factor (PRGF). The rhBMP-2 affected local bone augmentation with increasing volume for higher doses. Both rhPDGF and PRGF showed a positive effect in favor of the growth factor. Differing levels and quantity of evidence were noted to be available for the growth factors evaluated, revealing that rhBMP-2, rhPDGF, and PRGF may stimulate local bone augmentation to various conditions. Especially the potential of rhBMP-2 is supportive. However, the confined number of investigators using these techniques and the low number of patient treatments reported in the literature, the generalizability of this approach is limited at this time. © 2009 Japanese Association for Dental Science.

DOI： 10.1016/j.jdsr.2009.10.004

Scopus

The relationship between temporomandibular joint pathosis and muscle tenderness in the orofacial and neck/shoulder region. International journal

Eitoku Inoue, Kenji Maekawa, Hajime Minakuchi, Chiyomi Nagamatsu-Sakaguchi, Tsuyoshi Ono, Yoshizo Matsuka, Glenn T Clark, Takuo Kuboki

Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 109 ( 1 ) 86 - 90 2010.1

Language：English Publisher：MOSBY-ELSEVIER

OBJECTIVE: The objective of this study was to investigate the association between TMJ pain/disk pathosis and the muscle tenderness pattern in the orofacial and neck/shoulder region. STUDY DESIGN: One hundred seventy-one TMD patients were divided into 4 groups, including group 1: patients with painful unilateral TMJ disk displacement (DD); group 2: patients with painless unilateral TMJ DD; group 3: patients with painless bilateral TMJ DD; and group 4: patients with a bilateral normal TMJ disk position (n = 41). Each subject underwent muscle palpation and the side-by-side number of muscle tenderness points was combined as the number of muscle tenderness points on each side. Within each group, DD with and without reduction subjects were separated into subgroups and then were analyzed. RESULTS: In group 1, the median muscle tenderness points on the side with painful TMJ DD without reduction was significantly higher than on the normal side (P = .019), whereas the palpation scores for painless DD patients showed no significant difference between the DD and normal sides. CONCLUSIONS: These results indicated painful disk displacement to possibly be correlated with ipsilateral muscle tenderness.

DOI： 10.1016/j.tripleo.2009.07.050

Gene expression profile of mouse masseter muscle after repetitive electrical stimulation.

Tsuyoshi Ono, Kenji Maekawa, Wataru Sonoyama, Shunji Kojima, Tsutomu Tanaka, Glenn T Clark, Takuo Kuboki

Journal of prosthodontic research 54 ( 1 ) 36 - 41 2010.1

Language：English Publisher：ELSEVIER IRELAND LTD

PURPOSE: To examine gene expression profile changes in the mouse masseter muscle tissue after repetitive electrical stimulation by using a DNA microarray technique. METHODS: Nine male ICR mice aged 10 weeks were used. Each anesthetized mouse was secured on a platform in a supine position and the masseter muscle tissues on both sides were exposed. Bipolar electrodes were set on the right masseteric fascia to electrically stimulate the masseter muscle (8 V, 10 Hz, 20 ms) for 30 min. After cessation of stimulation bilateral masseter muscle tissues were sampled at 0 h (n=3), 1h (n=3), 2h (n=3). Total RNA was isolated from the homogenized muscle tissues and purified mRNA samples (50 microg) were processed and hybridized with microarray slides. Probe arrays were then scanned and analyzed to calculate the signal density. Gene expression profiles were compared at each time point between the right (stimulation side) and left (control side) masseter. When the gene expression levels were different more than 2-fold, the difference was regarded as positive. RESULTS: Of the 6400 genes assessed, 1733 genes were up-regulated and 515 genes were down-regulated in the stimulation side at least once during the experimental time course. These up- or down-regulated genes were associated with autoimmune/inflammatory disease (28/114), cardiovascular disease (17/61), neuroscience (12/50), apoptosis (27/93), diabetes/obesity (9/28), signal transduction (66/250) and others. 28 genes were up-regulated and 25 genes were down-regulated at all time points. CONCLUSIONS: Dramatic gene expression changes were induced by the repetitive electrical muscle stimulation in mouse masseter.

DOI： 10.1016/j.jpor.2009.09.001

研究室から診療室へ State of the art, Prosthodontics 臨床家が知っておきたい補綴最新トピックス（第6回）未来の補綴歯科を切り開くために.

携帯電話を応用したEMAによる口腔インプラント一次手術後急性痛の測定における測定間隔の検討

黒井隆太, 水口一, 川上彩, 松香芳三, 岡田宏基, 窪木拓男

日本補綴歯科学会誌 2 ( 1 ) E29 - E29 2010.1

頭部外傷後遷延性意識障害例の気道閉塞に対する緩衝機構付きスリープスプリントの効果

近藤康弘, 本田千穂, 窪木拓男, 縄稚久美子

日本補綴歯科学会誌 2 ( 1 ) E37 - E37 2010.1

窪木拓男

補綴臨床 43 ( 5 ) 555 - 565 2010

咬合崩壊を来した重度歯周炎患者に対する歯周・矯正・補綴専門医によるInterdisciplinary Approach

完山学, 本城正, 高柴正悟, 藪中友絵, 山本悠美子, 藤田泰弘, 山城隆, 窪木拓男

岡山歯学会雑誌 29 ( 1 ) 55 - 60 2010

Language：Japanese Publisher：岡山歯学会

21世紀の歯科医療は、開業医が主体となって行うprimary care dentistryと、大学病院などにおいて専門医同士がチームを組んで行うinterdisciplinary approach(以下IA)に二極化していくことが予想されている。また、最近では患者の訴えやニーズが多種多様であるため、患者個々に応じたオーダーメード治療が求められている。岡山大学病院の歯科系各診療科は、様々な学会の認定研修施設に認定されており、各科にはそれぞれの学会の認定医や専門医、さらにはそれらの指導医が名を連ねている。すなわち、当院は専門医同士のIAによるオーダーメード治療が容易に実践できる環境にある。今回、重度の歯周炎により咬合が崩壊している63歳男性患者に対し、歯周科、矯正科、補綴科の専門医が十分な連携の下でIAを行い、患者の高い満足度が得られたので報告した。

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