Faculty Profiles - KUBOKI Takuo

MISC - KUBOKI Takuo

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健常者における睡眠時の覚醒反応と随伴運動との関連

三木春奈, 水口一, 上枝麻友, 重本修伺, 重本修伺, 鈴木善貴, 前川賢治, 松香芳三, 窪木拓男

日本補綴歯科学会誌(Web) 7 2015

歯科介入型の新たな口腔管理法の開発及び介入効果の検証等に関する研究-超急性期病院の調査-

窪木拓男, 窪木拓男, 曽我賢彦

歯科介入型の新たな口腔管理法の開発及び介入効果の検証等に関する研究平成26年度総括・分担研究報告書 2015

The BMP2 antagonist inhibitor L51P enhances the osteogenic potential of BMP2 by simultaneous and delayed synergism

Hany Mohamed Khattab, Mitsuaki Ono, Wataru Sonoyama, Yasutaka Oida, Shigehiko Shinkawa, Yuya Yoshioka, Kenji Maekawa, Yasuhiko Tabata, Kazushige Sugama, Walter Sebald, Takuo Kuboki

BONE 69 165 - 173 2014.12

Language：English Publisher：ELSEVIER SCIENCE INC

Bone morphogenetic protein 2 (BMP2) is a potent osteoinductive cytokine that plays crucial roles in bone repair. However, large amounts of BMP2 are required to induce sufficient bone formation in humans possibly due to a feedback response of BMP antagonists. The engineered BMP2 variant L51P is deficient in BMP receptor type I activation but maintains affinity for BMP antagonists and can allow for the inactivation of BMP antagonists, and eventually enhance BMP2 action. As hypothesized, simultaneous addition of L51P enhanced the BMP2-induced osteogenesis. To test the ability of L51P to competitively inactivate BMP antagonists, cell binding affinity of BMP2 ligands was investigated in the presence or absence of L51P. Because the BMP antagonists were highly expressed 3 days after exogenous BMP2 stimulation, we collected supernatants from 3-day stimulated cell cultures and used as condition culture media (CM). The results showed a significant decrease in the cell binding of BMP2 ligands when cells were incubated with exogenous BMP2 and CM, whereas L51P addition competitively rescued the suppression of BMP2-to-cell binding induced by CM incubation. In a delayed experimental model, L51P was applied 3 days after exogenous BMP2 stimulation and we could observe a striking enhancement of the BMP2-induced SMAD-1/5/8 phosphorylation and luciferase activity of the Id1 promoter compared to the simultaneous addition of the two factors. These findings provide a deeper insight into the cellular and molecular mechanisms involved in the effect of L51P in suppressing the BMP antagonists and enhancing BMP activity. Additionally, these results demonstrate that L51P is a promising down regulator of BMP-induced negative feedback, which could have a significant impact in future applications of BMP2 in research and clinical settings. (C) 2014 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bone.2014.09.011

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炎症環境による歯髄細胞の幹細胞化歯髄細胞分化に与えるTNF-αの影響

上枝麻友, 藤澤拓生, 大野充昭, Hara Emilio Satoshi, Pham Thanh Hai, 園山亘, 窪木拓男, 松香芳三

日本再生歯科医学会誌 12 ( 1 ) 48 - 48 2014.12

マウス大腿骨の二次骨化中の組織石灰化に関する定性分析(Qualitative analysis of tissue mineralization during secondary ossification of mouse femur)

ハラ・エミリオ・サトシ, 岡田正弘, 服部高子, 久保田聡, 窪木拓男, 松本卓也

日本バイオマテリアル学会大会予稿集 36回 251 - 251 2014.11

少数歯欠損患者における補綴治療後5年経過時の口腔関連Quality of Lifeの評価

黒崎陽子, 大野彩, 瀧内博也, 佐伯真未子, 小山絵里, 三野卓哉, 前川賢治, 窪木拓男

日本口腔インプラント学会誌 27 ( 3 ) 386 - 386 2014.9

軟骨特異的CCN3過剰発現は軟骨の最終分化の遅延だけでなく、変形性関節症を誘発する

服部高子, 大野充昭, 星島光博, 角谷宏一, 窪木拓男, 滝川正春

Journal of Oral Biosciences Supplement 2014 125 - 125 2014.9

インプラント義歯に係わる臨床エビデンスと技工

窪木拓男

日本口腔インプラント学会誌 27 ( 3 ) 378 - 379 2014.9

要介護高齢者の認知症の重症度と口腔内環境およびBody Mass Indexの関連

小山絵理, 大野彩, 山本道代, 瀧内博也, 中川晋輔, 三野卓哉, 黒崎陽子, 縄稚久美子, 水口真実, 水口一, 前川賢治, 窪木拓男

老年歯科医学 29 ( 2 ) 117 - 118 2014.9

Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy. International journal

Masamitsu Oshima, Kaoru Inoue, Kei Nakajima, Tetsuhiko Tachikawa, Hiromichi Yamazaki, Tomohide Isobe, Ayaka Sugawara, Miho Ogawa, Chie Tanaka, Masahiro Saito, Shohei Kasugai, Teruko Takano-Yamamoto, Takashi Inoue, Katsunari Tezuka, Takuo Kuboki, Akira Yamaguchi, Takashi Tsuji

Scientific reports 4 6044 - 6044 2014.8

Language：English Publisher：NATURE PUBLISHING GROUP

Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy.

DOI： 10.1038/srep06044

Web of Science

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少数歯欠損患者におけるインプラント治療後6年経過時の口腔関連QOL評価

黒崎陽子, 大野彩, 小山絵理, 前川賢治, 窪木拓男

日本口腔インプラント学会誌 27 ( 特別号 ) 233 - 233 2014.8