Faculty Profiles - KUBOKI Takuo

MISC - KUBOKI Takuo

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【65歳以上の患者へのインプラント治療・管理ガイド要介護になっても対応できるために】(第1章)高齢者から信頼されるインプラント治療を行うために高齢者におけるインプラント治療を考える

窪木拓男, 大野彩, 園山亘, 荒川光

日本歯科評論 ( 別冊2014 ) 8 - 14 2014.6

出生後のイヌ永久歯胚組織を用いた器官原基法による完全な臓器としての歯の再生

大野充昭, 大島正充, 園山亘, 小川美帆, 笈田育尚, Hara Emilio S., 新川重彦, 中島隆, 辻孝, 窪木拓男

日本補綴歯科学会誌 6 ( 特別号 ) 103 - 103 2014.5

マウス歯胚発生過程におけるHox遺伝子の発現パターン

新川重彦, 内部健太, 大野充昭, 園山亘, 吉岡裕也, 植田淳二, 窪木拓男

日本補綴歯科学会誌 6 ( 特別号 ) 263 - 263 2014.5

少数歯欠損患者における補綴治療後6年経過時の口腔関連QOLの評価

黒崎陽子, 大野彩, 瀧内博也, 佐伯真未子, 小山絵理, 三野卓哉, 前川賢治, 窪木拓男

日本補綴歯科学会誌 6 ( 特別号 ) 307 - 307 2014.5

入院高齢者の口腔内環境や栄養状態、日常生活動作が生命予後、肺炎発症に及ぼす影響

藤原彩, 上原淳二, 水口一, 水口真実, 大野彩, 縄稚久美子, 前川賢治, 窪木拓男

日本補綴歯科学会誌 6 ( 特別号 ) 111 - 111 2014.5

軟骨分化におけるCCN4/WISP-1の機能解析

吉岡裕也, 大野充昭, 前田あずさ, 秋山謙太郎, 園山亘, 窪木拓男

日本補綴歯科学会誌 6 ( 特別号 ) 136 - 136 2014.5

要介護高齢者の口腔内環境および摂食状態と主たる介護者の介護負担感との関連

山本道代, 大野彩, 瀧内博也, 小山絵理, 中川晋輔, 三野卓哉, 黒崎陽子, 水口真実, 水口一, 前川賢治, 窪木拓男

日本補綴歯科学会誌 6 ( 特別号 ) 149 - 149 2014.5

短縮歯列への補綴介入に関する多施設共同研究咀嚼能力への治療効果

笛木賢治, 五十嵐順正, 吉田英子, 前田芳信, 池邉一典, 馬場一美, 塚崎弘明, 古谷野潔, 荻野洋一郎, 佐々木啓一, 小山重人, 赤川安正, 是竹克紀, 窪木拓男, 荒川光, 春日井昇平, 近藤尚知, 黒田真司

日本補綴歯科学会誌 6 ( 特別号 ) 181 - 181 2014.5

The regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarthritis models in vivo. International journal

Tarek Abd El Kader, Satoshi Kubota, Takashi Nishida, Takako Hattori, Eriko Aoyama, Danilo Janune, Emilio S Hara, Mitsuaki Ono, Yasuhiko Tabata, Takuo Kuboki, Masaharu Takigawa

Bone 59 ( 59C ) 180 - 8 2014.2

Language：English Publisher：ELSEVIER SCIENCE INC

The role of CCN family proteins has been proven to be of extreme importance in the process of cartilage development and endochondral ossification. The second member, CCN2, consists of 4 conserved modules that interact with a number of cofactors to display multiple functions. Although the potentially therapeutic effect of intact CCN2 on cartilage regeneration has been indicated by a number of studies, the regenerative effect of independent modules comprising CCN2 has never been evaluated before. This study aims to discover a more robust and effective CCN2 derivative to induce regeneration through assessing the effect of CCN2 independent modules on regeneration in vitro and in vivo, in comparison to the full length CCN2. In vitro evaluation using human chondrocytic cells showed a remarkable enhancing effect of several single modules on the gene expression of cartilaginous extracellular matrix components; whereas combinations of 2 or 3 modules rather diminished such effects. Interestingly, combination of all 4 modules redeemed the effect of intact CCN2 in vitro. Suspecting the re-assembly of the 4 modules, interaction among the modules was examined by surface plasmon resonance analysis. However, the results did not support the possible formation of a tetramodular complex. Next, the thrombospondin 1 type 1 repeat module (TSP1), which was found most promising in the experiments in vitro, and the combination of 4 modules were forwarded further to in vivo confirmation using 2 rat osteoarthritis (OA) models. As a result, TSP1 displayed more prominent regenerative effects than intact CCN2 on damaged cartilage. Unexpectedly, the combination of 4 modules showed limited effects in vivo. These results indicate the utility of TSP1 in the regenerative therapeutics of OA. Possible molecular mechanism that enables conditional reconstruction of CCN2 by 4 modules is discussed as well.

DOI： 10.1016/j.bone.2013.11.010

The pathophysiology of heterotopic ossification: Current treatment considerations in dentistry

Kengo Shimono, Kenta Uchibe, Takuo Kuboki, Masahiro Iwamoto

Japanese Dental Science Review 50 ( 1 ) 1 - 8 2014.2

Language：English Publishing type：Book review, literature introduction, etc.

Heterotopic ossification (HO) consists of the formation of ectopic cartilage followed by endochondral bone, and is triggered by major surgeries, large wounds, and other conditions. Daily functions of HO patients can be hampered by the loss of normal posture, pain, inflammation, reduced mobility, formation of pressure ulcers, deep venous thrombosis, and other complications. Research so far revealed the molecular and cellular pathways leading HO formation, and proposed several possible mechanisms behind such pathways. Nonsteroidal anti-inflammatory drug (NSAID) regimens and localized low-dose irradiation are currently available as prophylaxis of HO formation. However, they are not always effective and do not target skeletogenic processes directly. New therapeutic modalities targeting pathological process of HO formation, such as bone morphogenetic proteins (BMP) inhibitors like Noggin, BMP type 1 receptor inhibitor, and nuclear retinoic acid receptor-gamma (RARγ) agonists are currently under investigation. In this review, we will summarize our current understanding of the pathology and molecular and cellular mechanisms of HO, especially endochondral heterotopic ossification, and then discuss its current and future therapies. We will also discuss the potential application of heterotopic ossification in the dental field. © 2013 Japanese Association for Dental Science.

DOI： 10.1016/j.jdsr.2013.07.003

Scopus

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上顎洞底挙上術における大腸菌由来BMP-2/β-TCP複合体の有効性の検討

笈田育尚, 園山亘, 大野充昭, 新川重彦, 秋山謙太郎, 中島隆, 前川賢治, 窪木拓男

日本補綴歯科学会誌 6 ( 1 ) E5 - E5 2014.1

残存歯数と体重増加の関連企業歯科検診受診者を対象とした前向きコホート研究

瀧内博也, 大野彩, 荒川光, 三野卓哉, 上枝麻友, 藤原彩, 新川重彦, 中川晋輔, 正木明日香, 完山学, 松香芳三, 前川賢治, 窪木拓男

日本補綴歯科学会誌 6 ( 1 ) E4 - E4 2014.1

入院中の要介護高齢者の残存歯数、栄養状態、日常生活動作が生命予後に及ぼす影響

藤原彩, 上原淳一, 水口一, 水口真実, 大野彩, 縄稚久美子, 前川賢治, 窪木拓男

日本補綴歯科学会誌 6 ( 1 ) E4 - E4 2014.1

Mesenchymal Stem/Progenitor Cell Isolation from Tooth Extraction Sockets

Nakajima, R., Ono, M., Hara, E. S., Oida, Y., Shinkawa, S., Pham, H. T., Akiyama, K., Sonoyama, W., Maekawa, K., Kuboki, T.

Journal of Dental Research 93 ( 11 ) 1133 - 1140 2014

顎関節症はなぜ発症するのか

前川賢治, 窪木拓男

日本歯科評論 74 ( 1 ) 43 - 48 2014

Efficient Bone Formation in a Swine Socket Lift Model Using Escherichia coli-Derived Recombinant Human Bone Morphogenetic Protein-2 Adsorbed in beta-Tricalcium Phosphate

Mitsuaki Ono, Wataru Sonoyama, Katushi Yamamoto, Yasutaka Oida, Kentaro Akiyama, Shigehiko Shinkawa, Ryu Nakajima, Hai T. Pham, Emilio S. Hara, Takuo Kuboki

CELLS TISSUES ORGANS 199 ( 4 ) 249 - 255 2014

Language：English Publisher：KARGER

Several preclinical studies have shown that Escherichia coli-derived bone morphogenetic protein-2 (E-BMP-2) is as effective as mammalian cell-derived bone morphogenetic protein-2 (C-BMP-2) in the treatment of bone defects. However, further investigation of the effectiveness and determination of the optimal dosage of E-BMP-2 in large animals are still necessary before its full application in humans. This study investigated the efficiency of different concentrations of E-BMP-2 adsorbed in beta-TCP for bone augmentation and osseo-integration of immediate dental implants in a swine socket lift model. Following exposure of the maxillary sinus lateral wall, a 3.4-mm (diameter) cavity was drilled and filled with 0.1 g of beta-TCP containing different doses of E-BMP-2 (0, 10, 30, or 100 mu g/site) to lift the Schneiderian membrane. A dental implant was then immediately inserted. Bone-to-implant contact (BIC) and bone density (BD) examined via histological analysis were used as parameters to assess E-BMP-2 efficiency in bone formation. The implant stability quotient (ISQ) was measured using Osstell to determine the effect of E-BMP-2/beta-TCP on implant stability. After 8 weeks, the groups that received 30 and 100 mu g of E-BMP-2 showed substantial new bone formation in the elevated space, while no bone formation was observed with beta-TCP alone. Accordingly, BIC and BD presented a dose-dependent response to in-creasing doses of E-BMP-2. However, there was no increase in implant stability with E-BMP-2 treatment. In conclusion, the E-BMP-2/beta-TCP combination was efficient in bone formation and osseointegration of dental implants in a socket lift model in mini-pigs. (C) 2015 S. Karger AG, Basel

DOI： 10.1159/000369061

Web of Science

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軟骨特異的CCN3過剰発現は軟骨の最終分化の遅延だけでなく,変形性関節症を誘発する

服部高子, 大野充昭, 星島光博, 星島光博, 角谷宏一, 窪木拓男, 滝川正春

Journal of Oral Biosciences Supplement (Web) 2014 2014

歯科介入型の新たな口腔管理法の開発及び介入効果の検証等に関する研究周術親等の口腔内管理の開発及び介入効果の検証

窪木拓男, 窪木拓男, 曽我賢彦

歯科介入型の新たな口腔管理法の開発及び介入効果の検証等に関する研究平成25年度総括・分担研究報告書 2014

リジンオリゴマーからなる関節軟骨特異的X線プローブの創出

大橋俊孝, 芳谷学, 大野充昭, 山田翔也, 大月孝志, 前原亜美, 廣畑聡, 西田圭一郎, 窪木拓男, 加来田博貴, 二宮善文

日本軟骨代謝学会プログラム・抄録集 27th 2014

いま,顎関節症にどう取り組むべきか 2 顎関節症はなぜ発症するのか

前川賢治, 窪木拓男

日本歯科評論 ( 855 ) 2014

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