Faculty Profiles - KUBOKI Takuo

MISC - KUBOKI Takuo

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大腸菌由来・遺伝子組換BMP-2と塩基性ゼラチンを用いた骨補填材の効果

下野賢吾, 大島正充, 完山学, 大野充昭, 縄稚久美子, 田畑泰彦, Sebald Walter, 洲鎌和茂, 窪木拓男

日本歯科医師会雑誌 61 ( 5 ) 513 - 513 2008.8

Relationship between the frequency of sleep bruxism and the prevalence of signs and symptoms of temporomandibular disorders in an adolescent population International journal

Chiyomi Nagamatsu-Sakaguchi, Hajime Minakuchi, Glenn T. Clark, Takuo Kuboki

INTERNATIONAL JOURNAL OF PROSTHODONTICS 21 ( 4 ) 292 - 298 2008.7

Language：English Publisher：QUINTESSENCE PUBLISHING CO INC

Purpose: The relationship between sleep bruxism (SB) and temporomandibular disorders (TMD) is unclear. This study aimed to estimate SB prevalence in an adolescent population and to investigate the relationship between SB frequency and prevalence of TMD signs/symptoms. Materials and Methods: First-year students at a high school in Okayama, Japan, were recruited in 2005, with 195 subjects responding. The SB detection device was a miniature disposable device (BiteStrip, SLP) that indicated the total SB events per night on a 4-grade score. The subjects were divided into severe and nonsevere SB groups with SB frequency cutoffs. The subjects were examined for temporomandibular joint (TMJ) noise during mouth opening/closing, tenderness of the masticatory/cervical muscles, and range of TMJ condylar movement. The presence/absence of headache and shoulder stiffness was also determined by the interview. The odds ratio (OR) and confidence interval (CI) were calculated to test the relationship between SB frequency, gender difference, and presence of the TMD signs/symptoms by multiple regression analysis. Results: Severe SB (more than 125 events per night) was significantly related to the presence of TMJ clicking (OR: 3.74, CI: 1.22-11.49, P =.02), while gender (male) was not related to the presence of TMJ clicking. Severe SB was not related to headache, though gender (male) was significantly related to headache (OR: 2.52, CI: 1.04-6.11, P = .04) in these subjects. Conclusion: These results suggest that the presence of TMJ clicking was closely related to severe SB in an adolescent population.

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CCN family 2/connective tissue growth factor (CCN2/CTGF) stimulates proliferation and differentiation of auricular chondrocytes

T. Fujisawa, T. Hattori, M. Ono, J. Uehara, S. Kubota, T. Kuboki, M. Takigawa

OSTEOARTHRITIS AND CARTILAGE 16 ( 7 ) 787 - 795 2008.7

Language：English Publisher：W B SAUNDERS CO LTD

Objectives: CCN family 2/connective tissue growth factor (CCN2/CTGF) is an atypical growth factor for growth plate chondrocytes. It plays an important role in their proliferation and differentiation in vitro, but does not stimulate hypertrophy or calcification of articular chondrocytes. We herein report for the first time that CCN2/CTGF promotes growth and differentiation of auricular chondrocytes and maintains their molecular phenotype in vitro and in vivo.
Methods: Auricular chondrocytes were isolated from rabbit auricular cartilage by trypsin-collagenase treatment, and treated with human recombinant CCN2/CTGF or infected with adenovirus harboring the ccn2/ctgf gene. Cell proliferation was measured by [3 H] thymidine incorporation and MTS assay, and changes in gene expression of auricular chondrocyte markers were monitored by real-time polymerase chain reaction, Northern hybridization, and histological analysis. For in vivo studies, auricular chondrocytes were cultured as pellets and implanted subcutaneously after treatment of recombinant human CCN2/CTGF. Ectopically formed cartilage was subjected to histological analysis. Cell death was monitored by in situ TUNEL analysis.
Results: CCN2/CTGF stimulated proliferation, differentiation and synthesis of elastin and proteoglycans of rabbit primary auricular chondrocytes in a dose-dependent manner. CCN2/CTGF caused a 2.5-fold increase in the expression of elastin in comparison to the control, resulting in enhanced deposition of elastin fibers in a monolayer culture of auricular chondrocytes. Mineralization was not induced; in contrast, CCN2/CTGF stimulated expression of matrix gla protein which is known to impair mineralization. Furthermore, pretreatment of pellets of auricular chondrocytes with CCN2/CTGF and subcutaneous implantation significantly enhanced the growth of ectopic auricular cartilage pieces expressing phenotypic markers of auricular chondrocytes including type 11 and X collagen. Notably, chondrocyte apoptosis was impaired by CCN2/CTGF.
Conclusions: These findings show that CCN2/CTGF may be a suitable agent for promoting differentiation and growth of auricular chondrocytes, while preventing mineralization and apoptosis, and suggests that CCN2/CTGF may be useful for the repair or reconstruction Of Elastic cartilage. (C) 2007 Ostecarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.joca.2007.11.001

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表面麻酔薬による歯根膜感覚への影響

平井真也, 小川匠, 松香芳三, 重田優子, 安藤栄里子, 平林里大, 井川知子, 窪木拓男, 福島俊士

日本顎関節学会雑誌 20 ( Suppl. ) 104 - 104 2008.7

bFGF局所投与における顎骨骨質改善ウサギモデルにおける組織学的・細胞生物学的検討

大島正充, 園山亘, 大野充昭, 下野賢吾, 暈貴行, 岡本洋介, 土本洋平, 松香芳三, 窪木拓男

Inflammation and Regeneration 28 ( 4 ) 335 - 335 2008.7

The effect of peripheral administration of botulinum toxin type A in rats with neuropathic pain

Yoshizo Matsuka, Yoichi Kitamura, Igor Spigelman, Rahena Akther, Yumiko Yamamoto, Wataru Sonoyama, Keiji Oguma, Takuo Kuboki

TOXICON 51 7 - 7 2008.6

【審美歯科・インプラントワードブック】ベーシック補綴全身とQOL 全身疾患との関係

野田欣志, 荒川光, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 138 - 139 2008.6

【審美歯科・インプラントワードブック】インプラント補綴治療の実際インプラントの印象法

山崎聖也, 荒川光, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 62 - 63 2008.6

【審美歯科・インプラントワードブック】審美補綴審美修復の実際ホワイトニング

上原淳二, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 98 - 101 2008.6

【審美歯科・インプラントワードブック】審美補綴審美修復の実際カントゥアと歯肉

荒川光, 小田師巳, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 106 - 109 2008.6

【審美歯科・インプラントワードブック】審美補綴審美修復の実際オベイトポンティックとハーフポンティック

園山亘, 金田貴哲, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 112 - 113 2008.6

【審美歯科・インプラントワードブック】インプラント補綴基礎知識とコンセプト治療成績

荒川光, 野田欣志, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 16 - 17 2008.6

【審美歯科・インプラントワードブック】審美補綴審美修復の実際審美補綴のためのサージェリー

完山学, 山崎章弘, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 114 - 117 2008.6

【審美歯科・インプラントワードブック】審美補綴審美修復の実際オパール効果・イリュージョン効果

荒川光, 上田明広, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 118 - 119 2008.6

被検者の自覚や家族の指摘のみで睡眠時ブラキシズムは診断できるか?

坂口千代美[永松], 水口一, Clark Glenn T., 窪木拓男

岡山歯学会雑誌 27 ( 1 ) 68 - 68 2008.6

【審美歯科・インプラントワードブック】ベーシック補綴全身とQOL QOL/Quality of Life

木村彩, 荒川光, 窪木拓男

補綴臨床別冊 ( 審美歯科・インプラントワードブック ) 148 - 149 2008.6

CCNファミリー2/結合組織成長因子(CCN2/CTGF)が骨芽細胞において骨形成因子(BMP)-2刺激による骨芽細胞分化を修飾する

前田あずさ, 西田崇, 川木晴美, 久保田聡, 窪木拓男, 滝川正春

岡山歯学会雑誌 27 ( 1 ) 63 - 64 2008.6

Novel transcription-factor-like function of human matrix metalloproteinase 3 regulating the CTGF/CCN2 gene. International journal

Takanori Eguchi, Satoshi Kubota, Kazumi Kawata, Yoshiki Mukudai, Junji Uehara, Toshihiro Ohgawara, Soichiro Ibaragi, Akira Sasaki, Takuo Kuboki, Masaharu Takigawa

Molecular and cellular biology 28 ( 7 ) 2391 - 413 2008.4

Language：English Publisher：AMER SOC MICROBIOLOGY

Matrix metalloproteinase 3 (MMP3) is well known as a secretory endopeptidase that degrades extracellular matrices. Recent reports indicated the presence of MMPs in the nucleus (A. J. Kwon et al., FASEB J. 18:690-692, 2004); however, its function has not been well investigated. Here, we report a novel function of human nuclear MMP3 as a trans regulator of connective tissue growth factor (CCN2/CTGF). Initially, we cloned MMP3 cDNA as a DNA-binding factor for the CCN2/CTGF gene. An interaction between MMP3 and transcription enhancer dominant in chondrocytes (TRENDIC) in the CCN2/CTGF promoter was confirmed by a gel shift assay and chromatin immunoprecipitation. The CCN2/CTGF promoter was activated by overexpressed MMP3, whereas a TRENDIC mutant promoter lost the response. Also, the knocking down of MMP3 suppressed CCN2/CTGF expression. By cytochemical and histochemical analyses, MMP3 was detected in the nuclei of chondrocytic cells in culture and also in the nuclei of normal and osteoarthritic chondrocytes in vivo. The nuclear translocation of externally added recombinant MMP3 and six putative nuclear localization signals in MMP3 also were shown. Furthermore, we determined that heterochromatin protein gamma coordinately regulates CCN2/CTGF by interacting with MMP3. The involvement of this novel role of MMP3 in the development, tissue remodeling, and pathology of arthritic diseases through CCN2/CTGF regulation thus is suggested.

DOI： 10.1128/MCB.01288-07

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専用ホームページならびにインターネット掲示板を利用した補綴スキル教育における学生・教官相互コミュニケーション

水口一, 暈貴行, 内部健太, 北村洋一, 前田あずさ, 永松千代美, 上原淳二, 窪木拓男

日本補綴歯科学会雑誌 52 ( 2 ) 262 - 262 2008.4

安静時ヒト咬筋血流動態に対する直線偏光近赤外線照射の影響

瀧口悟, 前川賢治, 小野剛, 窪木拓男

日本顎関節学会雑誌 20 ( 1 ) 39 - 39 2008.4

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