Faculty Profiles - KUBOKI Takuo

MISC - KUBOKI Takuo

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大腸菌由来rhBMP-2を応用した自己骨再生能を持った歯科用GBRメンブレンの開発

大野充昭, 園山亘, 笈田育尚, 正木明日香, 新川重彦, 中島隆, 松香芳三, 窪木拓男

日本補綴歯科学会誌 5 ( 1 ) E115 - E115 2013.1

OstemiR: a novel panel of microRNA biomarkers in osteoblastic and osteocytic differentiation from mesencymal stem cells. International journal

Takanori Eguchi, Ken Watanabe, Emilio Satoshi Hara, Mitsuaki Ono, Takuo Kuboki, Stuart K Calderwood

PloS one 8 ( 3 ) e58796 2013

Language：English Publisher：PUBLIC LIBRARY SCIENCE

MicroRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate cell behavior through inhibition of translation from mRNA to protein, promotion of mRNA degradation and control of gene transcription. In this study, we investigated the miRNA expression signatures of cell cultures undergoing osteoblastic and osteocytic differentiation from mesenchymal stem cells (MSC) using mouse MSC line KUSA-A1 and human MSCs. Ninety types of miRNA were quantified during osteoblastic/osteocytic differentiation in KUSA-A1 cells utilizing miRNA PCR arrays. Coincidently with mRNA induction of the osteoblastic and osteocytic markers, the expression levels of several dozen miRNAs including miR-30 family, let-7 family, miR-21, miR-16, miR-155, miR-322 and Snord85 were changed during the differentiation process. These miRNAs were predicted to recognize osteogenic differentiation-, stemness-, epinegetics-, and cell cycle-related mRNAs, and were thus designated OstemiR. Among those OstemiR, the miR-30 family was classified into miR-30b/c and miR-30a/d/e groups on the basis of expression patterns during osteogenesis as well as mature miRNA structures. In silico prediction and subsequent qRT-PCR in stable miR-30d transfectants clarified that context-dependent targeting of miR-30d on known regulators of bone formation including osteopontin/spp1, lifr, ccn2/ctgf, ccn1/cyr61, runx2, sox9 as well as novel key factors including lin28a, hnrnpa3, hspa5/grp78, eed and pcgf5. In addition, knockdown of human OstemiR miR-541 increased Osteopontin/SPP1 expression and calcification in hMSC osteoblastic differentiation, indicating that miR-541 is a negative regulator of osteoblastic differentiation. These observations indicate stage-specific roles of OstemiR especially miR-541 and the miR-30 family on novel targets in osteogenesis.

DOI： 10.1371/journal.pone.0058796

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実物大臓器立体モデルを活用した手術支援の実際と歯科技工士の関わり

竹内哲男, 有地秀裕, 松本洋, 中原龍一, 水川展吉, 吉岡徳枝, 木股敬裕, 尾崎敏文, 飯田征二, 佐々木朗, 三野卓哉, 前川賢治, 森田学, 槇野博史, 窪木拓男

QDT別冊 CAD/CAM YEAR BOOK 2013 別冊 22 - 31 2013

矯正歯科治療患者の口腔関連Quality of Lifeの評価

古森紘基, 川邊紀章, 片岡伴記, 白賀のり子, 大野彩, 窪木拓男, 山城隆

岡山歯学会誌 31 ( 2 ) 96 - 97 2013

miRNA-720 controls stem cell phenotype, proliferation and differentiation of human dental pulp cells. International journal

Emilio Satoshi Hara, Mitsuaki Ono, Takanori Eguchi, Satoshi Kubota, Hai Thanh Pham, Wataru Sonoyama, Shoji Tajima, Masaharu Takigawa, Stuart K Calderwood, Takuo Kuboki

PloS one 8 ( 12 ) e83545 2013

Language：English Publisher：PUBLIC LIBRARY SCIENCE

Dental pulp cells (DPCs) are known to be enriched in stem/progenitor cells but not well characterized yet. Small non-coding microRNAs (miRNAs) have been identified to control protein translation, mRNA stability and transcription, and have been reported to play important roles in stem cell biology, related to cell reprogramming, maintenance of stemness and regulation of cell differentiation. In order to characterize dental pulp stem/progenitor cells and its mechanism of differentiation, we herein sorted stem-cell-enriched side population (SP) cells from human DPCs and periodontal ligament cells (PDLCs), and performed a locked nucleic acid (LNA)-based miRNA array. As a result, miR-720 was highly expressed in the differentiated main population (MP) cells compared to that in SP cells. In silico analysis and a reporter assay showed that miR-720 targets the stem cell marker NANOG, indicating that miR-720 could promote differentiation of dental pulp stem/progenitor cells by repressing NANOG. Indeed, gain-and loss-of-function analyses showed that miR-720 controls NANOG transcript and protein levels. Moreover, transfection of miR-720 significantly decreased the number of cells positive for the early stem cell marker SSEA-4. Concomitantly, mRNA levels of DNA methyltransferases (DNMTs), which are known to play crucial factors during stem cell differentiation, were also increased by miR-720 through unknown mechanism. Finally, miR-720 decreased DPC proliferation as determined by immunocytochemical analysis against ki-67, and promoted odontogenic differentiation as demonstrated by alizarin red staining, as well as alkaline phosphatase and osteopontin mRNA levels. Our findings identify miR-720 as a novel miRNA regulating the differentiation of DPCs.

DOI： 10.1371/journal.pone.0083545

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A case of patient with neuropathic tooth pain who showed a clear response to pregabalin intake, judged from the longitudinal assessment of actual medicine consumption record

前川賢治, 古味佳子, 窪木拓男

日本口腔顔面痛学会雑誌 6 ( 1 ) 13 - 17 2013

神経障害性疼痛へのボツリヌス毒素の効果

松香芳三, 丸濵功太郎, 三宅愛, 山本由弥子, 前川賢治, 古味佳子, 園山亘, 小熊惠二, 窪木拓男

歯界展望 65 ( 5 ) 666 - 666 2013

Identification of risk factors for fracture of veneering materials and screw loosening of implant-supported fixed partial dentures in partially edentulous cases

Noda, K., Arakawa, H., Maekawa, K., Hara, E. S., Yamazaki, S., Kimura-Ono, A., Sonoyama, W., Minakuchi, H., Matsuka, Y., Kuboki, T.

Journal of Oral Rehabilitation 40 ( 3 ) 214 - 220 2013

A novel vibratory stimulation-based occlusal splint for alleviation of TMD painful symptoms: a pilot study

Hara, E. S., Witzel, A. L., De Luca, C. E. P., Ballester, R. Y., Kuboki, T., Bolzan, M. C.

Journal of Oral Rehabilitation 40 ( 3 ) 179 - 184 2013

A Retrospective Comparative 8-Year Study of Cumulative Complications in Teeth Adjacent to Both Natural and Implant-Supported Fixed Partial Dentures International journal

Yamazaki, Seiya, Arakawa, Hikaru, Maekawa, Kenji, Noda, Kinji, Hara, Emilio Satoshi, Minakuchi, Hajime, Sonoyama, Wataru, Matsuka, Yoshizo, Kuboki, Takuo

International Journal of Prosthodontics 26 ( 3 ) 260 - 264 2013

Language：English Publisher：QUINTESSENCE PUBLISHING CO INC

Purpose: To compare the complication rate of natural teeth adjacent to implant-supported dentures (IFDs) with that of teeth serving as abutments for fixed partial dentures (FPDs). The second goal was to assess the risk factors for complications in teeth adjacent to bounded edentulous spaces. Materials and Methods: The study subjects were selected from patients who received prosthodontic treatment for their bounded edentulous space not exceeding two missing teeth between February 1990 and March 2007. Sixty-one patients were included in the IFD group and 66 patients were included in the FPD group. Tooth complications were defined as tooth extraction, periodontal lesion, periapical lesion, and loss of prosthesis and were assessed by one examiner based on dental records. Results: The 8-year cumulative complication rate for the IFD group (7.9%) was significantly lower than for the FPD group (40.7%). Additionally, the 8-year cumulative complication rate of vital teeth (6%) was significantly lower than that of nonvital teeth (45.9%). A cox proportional hazard analysis revealed that nonvitality of dental pulp was a significant risk factor for tooth complications, whereas treatment modality was not. Conclusions: Teeth adjacent to IFD-treated edentulous spaces presented fewer complications than natural teeth serving as abutments for FPDs. Conservation of teeth adjacent to edentulous spaces as vital teeth was the key finding to limit further tooth loss.

DOI： 10.11607/ijp.3120

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Clinical Applications of Computerized Bone Models Made by 3D Plaster Printing

吉本彰夫, 吉本彰夫, 十河基文, 荒川光, 植村修作, 窪木拓男

日本口腔インプラント学会誌 26 ( 4 ) 2013

CCN4/WISP-1の骨形成における役割

大野充昭, 前田あずさ, 前田あずさ, 正木明日香, 吉岡裕也, 園山亘, 窪木拓男, YOUNG Marian F.