Faculty Profiles - OZAKI Toshifumi

MISC - OZAKI Toshifumi

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Evaluation of fracture line separation for PFNA blade insertion in femoral trochanteric fractures

TERAMOTO H., INOUE A., NAKANISHI K., NARAZAKI S., YAMANE K., NODA T., OZAKI T.

32 ( 1 ) 136 - 138 2010.2

Surgical Treatment for Vertical Talus Combined with Polysyndactyly : A Case Report

ENDO Hirosuke

19 ( 1 ) 16 - 20 2010.2

GDF-5/7 and bFGF Activate Integrin alpha 2-Mediated Cellular Migration in Rabbit Ligament Fibroblasts

Hirokazu Date, Takayuki Furumatsu, Yoshimasa Sakoma, Aki Yoshida, Yuko Hayashi, Nobuhiro Abe, Toshifumi Ozaki

JOURNAL OF ORTHOPAEDIC RESEARCH 28 ( 2 ) 225 - 231 2010.2

Language：English Publisher：JOHN WILEY & SONS INC

Cellular activities responding to growth factors are important in ligament healing. The anterior cruciate ligament (ACL) has poor healing potential compared to the medial collateral ligament (MCL). To assess the differences, we investigated the proliferation, migration, adhesion, and matrix synthesis responding to growth factors in rabbit ACL and MCL fibroblasts. ACL cell proliferation to basic fibroblast growth factor (bFGF), bone morphogenetic protein-2, growth and differentiation factor (GDF)-5, and GDF-7 treatment was similar to that of MCL cells. GDF-5 enhanced Colla1 expression in ACL and MCL fibroblasts up to 4.7- and 17-fold levels of control, respectively. MCL fibroblasts showed stronger migration activities in response to bFGF and GDF-5 than ACL cells. GDF-5/7 and bFGF also changed the stress fiber formation and cellular adhesion by modulating the distribution of integrin alpha 2. Functional blocking analyses using anti-integrin alpha 2 antibodies revealed that cellular migration responding to growth factors depended on the integrin alpha 2-mediated adhesion on type I collagen. The expression of integrin alpha 2 was also increased by growth factors in both cells. Our results demonstrate that GDF-5/7 and bFGF stimulate cellular migration by modulating integrin alpha 2 expression and integrin alpha 2-dependent adhesion, especially in MCL fibroblasts. These findings suggest that the different healing potential between ACL and MCL may be caused by different cellular behavior in the integrin alpha 2-mediated cellular migration in response to growth factors. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:225-231, 20.10

DOI： 10.1002/jor.20981

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創外固定を用いて加療した手関節否定型抗酸菌症の1例

島村安則, 野田知之, 雑賀建多, 門田康孝, 西田圭一郎, 尾﨑敏文

日本創外固定・骨延長学会誌 21 186 - 186 2010

Interventional radiology for vascular anomalies

三村秀文, 藤原寛康, 平木隆夫, 郷原英夫, 芝本健太郎, 木股敬裕, 尾崎敏文, 佐々木了, 金澤右

岡山医学会雑誌 122 ( 1 ) 2010

Biomechanical evaluation of the fixation methods for transcondylar fracture of the humerus:ONI plate versus conventional plates and screws.

Yasunori Shimamura, Keiichiro Nishida, Junya Imatani, Tomoyuki Noda, Hiroyuki Hashizume, Aiji Ohtsuka, Toshifumi Ozaki

Acta medica Okayama 64 ( 2 ) 115 - 120 2010

Language：English

We biomechanically evaluated the bone fixation rigidity of an ONI plate (Group I) during fixation of experimentally created transcondylar humerus fractures in cadaveric elbows, which are the most frequently observed humeral fractures in the elderly, and compared it with the rigidity achieved by 3 conventional fixation methods:an LCP reconstruction plate 3.5 using a locking mechanism (Group II), a conventional reconstruction plate 3.5 (CRP) with a cannulated cancellous screw (Group III), and a CRP with 2 cannulated cancellous screws (CS) in a crisscross orientation (Group IV). In the axial loading test, the mean failure loads were:Group I, 98.9+/-32.6;Group II, 108.5+/-27.2;Group III, 50.0+/-7.5;and Group IV, 34.5+/-12.2 (N). Group I fixations failed at a significantly higher load than those of Groups III and IV (p<0.05). In the extension loading test, the mean failure loads were:Group I, 34.0+/-12.4;Group II, 51.0+/-14.8;Group III, 19.3+/-6.0;and Group IV, 14.7+/-3.1 (N). Group IV fixations showed a significantly lower failure load than those of Group I (p<0.05). The fixation rigidities against mechanical loading by the ONI plate and LCP plate were comparable. These results suggested that an ONI system might be superior to the CRP and CS method, and comparable to the LCP method in terms of fixation rigidity for distal humerus fractures.

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Time-concentration profile of serum etanercept in Japanese patients with rheumatoid arthritis after treatment discontinuation before orthopedic surgery

NISHIDA Keiichiro, HASHIZUME Kenzo, KADOTA Yasutaka, NATSUMEDA Masamitsu, NAKAHARA Ryuichi, SAITO Taichi, KANAZAWA Tomoko, EZAWA Kazuhiko, OZAKI Toshihumi

20 ( 6 ) 637 - 639 2010

血管腫・血管奇形に対するinterventional radiology

三村秀文, 藤原寛康, 平木隆夫, 郷原英夫, 芝本健太郎, 木股敬裕, 尾﨑敏文, 佐々木了, 金澤右

岡山医学会雑誌 122巻 ( 1号 ) 55 - 59 2010

Radiographic measurements in the evaluation and classification of elbow joint destruction in patients with rheumatoid arthritis. International journal

Kenzo Hashizume, Keiichiro Nishida, Kazuo Fujiwara, Yasutaka Kadota, Ryuichi Nakahara, Kazuhiko Ezawa, Hajime Inoue, Toshifumi Ozaki

Clinical rheumatology 29 ( 6 ) 637 - 643 2010

Language：English

We developed two new radiographic parameters-the humeral surface height ratio and ulnar surface height ratio-to precisely detect changes in the bony structure of rheumatoid elbows. Of the 59 patients with rheumatoid arthritis, 101 elbows were classified into four types (osteoarthritis, ankylosis, erosive, and resorptive) according to the radiographic appearance. Clinically, osteoarthritis type and ankylosis type were considered to be stable form, and erosive type and resorptive type were unstable form. Patients' clinical data and yearly radiographic changes in the bony structure evaluated by the humeral surface height ratio and ulnar surface height ratio were compared among the four types and between the two forms. There were significant differences between the two forms and among the three types except for the ankylosis type in yearly radiographic changes in the bony structure evaluated by the humeral surface height ratio and ulnar surface height ratio. Stable and unstable forms were distinguished by a cut-off point of 0.65 and 2.58 in yearly radiographic changes in the bony structure evaluated by the humeral surface height ratio and the ulnar surface height ratio, respectively. These parameters might be useful for monitoring the structural changes of the elbow joint in rheumatoid arthritis.

DOI： 10.1007/s10067-010-1381-y

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2P1-G21 Tool Path Generation Method for High-speed Bone Cutting in Joint Replacement Assisting System

Bekku Akira, Nakano Taiga, Sugita Naohiko, Nakajima Yoshikazu, Kato Takeharu, Hujiwara Kazuo, Abe Nobuhito, Ozaki Toshifumi, Suzuki Masahiko, Inoue Takayuki, Kuramoto Kouiti, Nakashima Yoshio, Mitsuishi Mamoru

2010 "2P1 - G21(1)"-"2P1-G21(3)" 2010

Language：Japanese Publisher：The Japan Society of Mechanical Engineers

Multi-axis medical machine tool enables high-accuracy bone cutting in joint replacement assisting system. Short-time bone cutting is considered to be challenging problems in Joint Replacement Assisting System with multi-axis medical machine tool. The objective of this study is to propose tool path generation method for cutting down the time to form mounting surface for artificial joint and minimizing the collision of cutting tool with soft tissue. Short-time bone cutting decreases the danger of the infectious disease, and decreases the amount of blood under the operation. Software is developed for the application and the effectiveness is evaluated with a cadaveric bone.

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人工膝関節置換術に対する独自開発ナビゲーションシステムの精度評価

横山裕介, 藤原一夫, 阿部信寛, 西田圭一郎, 尾崎敏文, 鈴木昌彦, 中島義和, 斎藤季, 杉田直彦, 光石衛, 井上貴之, 藏本孝一

日本整形外科学会雑誌 84 ( 8 ) 2010

関節構成体機械特性測定プローブの開発

杉田直彦, 木崎通, 菅野大輔, 光石衛, 阿部信寛, 横山裕介, 尾崎敏文

精密工学会大会学術講演会講演論文集 2010 2010

The ACCURACY OF A ROBOT SYSTEM FOR LESS INVASIVE KNEE ARTHROPLASTY

FUJIWARA K., ABE N., NISHIDA K., OZAKI T., SUZUKI M., SAITO T., SUGITA N., NAKAJIMA Y., MITSUISHI M., INOUE T., KURAMOTO K., NAKASHIMA Y.

11 ( 3 ) 143 - 144 2009.11

Robotic Surgical System for the Minimally Invasive Knee Arthroplasty

MITSUISHI M., SUGITA N., NAKAJIMA Y., SAITO T., INOUE T., KURAMOTO K., NAKASHIMA Y., TANIMOTO K., FUJIWARA K., ABE N., OZAKI T., SUZUKI M.

11 ( 3 ) 157 - 159 2009.11

Open Reduction for Untreated Developmental Dislocation in the Hip for Infants Older than 3 Years

MINAGAWA Hiroshi

18 ( 2 ) 277 - 281 2009.9

Optical imaging of mouse articular cartilage using the glycosaminoglycans binding property of fluorescent-labeled octaarginine

K. Inagawa, T. Oohashi, K. Nishida, J. Minaguchi, T. Tsubakishita, K. O. Yaykasli, A. Ohtsuka, T. Ozaki, T. Moriguchi, Y. Ninomiya

OSTEOARTHRITIS AND CARTILAGE 17 ( 9 ) 1209 - 1218 2009.9

Language：English Publisher：W B SAUNDERS CO LTD

Objective: The aim of the current study was to examine the cartilage-specific binding property of polyarginine peptides (R4, 8, 12, and 16) and specifically to test octaarginine peptides for the optical imaging of articular cartilage in experimentally induced arthritis in mice.
Methods: Four rhodamine-labeled polyarginine peptides each with a different-length arginine chain (R4, 8, 12, or 16) were injected into the knee joints of C57BL/6J mice (n = 20). The joints were excised 1 h later and the fluorescent signal intensity in cartilage cryosections was compared for the four peptides. To examine the substrate of R8 in cartilage, femoral condyles obtained from another set of mice were treated with chondroitinase ABC (Ch'ase ABC), keratanase or heparitinase then immersed in R8-rhodamine. Fluorescent signals were examined by fluorescent microscopy. Next, R8-rhodamine was injected into the right knee joints of three control and three collagen antibody-induced arthritis (CAIA) mice, and fluorescent intensity in normal and degenerative cartilage was semi-quantitatively analysed on the histological sections using image software. Finally, femoral condyles from normal mice (n = 2) and CAIA mice (n = 2) were immersed in R8-rhodamine and calcein, then imaged using optical projection tomography (OPT).
Results: Fluorescent signals were specifically detected in the cartilage pericellular matrix from the surface to the tide mark but were completely absent in the calcified layer or bone marrow. The number of arginine residues significantly influenced peptide accumulation in articular cartilage, with R8 accumulating the most. The fluorescent signal in the femoral condylar cartilage diminished when it was treated with Ch'ase ABC. R8 accumulation was significantly decreased in the degenerative cartilage of CAIA mice, and this was demonstrated both histologically and in three-dimensional (3D)-reconstruction image by OPT.
Conclusion: R8 may be a useful new experimental probe for optical imaging of normal and arthritic articular cartilage. (C) 2009 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.joca.2009.03.010

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Usefulness of a navigation system in surgery for scoliosis: segmental pedicle screw fixation in the treatment

Kazuo Nakanishi, Masato Tanaka, Haruo Misawa, Yoshihisa Sugimoto, Tomoyuki Takigawa, Toshifumi Ozaki

ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY 129 ( 9 ) 1211 - 1218 2009.9

Language：English Publisher：SPRINGER

Postoperative outcomes of segmental pedicle screw fixation were evaluated in posterior scoliosis surgery with the use of navigation system.
We report the usefulness of a navigation system and a segmental pedicle screw fixation in surgery for scoliosis.
Few reports on a segmental pedicle screw fixation method for scoliosis surgery using a navigation system have been published. This is the report on the usefulness of a navigation system in segmental pedicle screw fixation.
We targeted 16 cases in which segmental pedicle screw fixation had been performed using a navigation system at our hospital. We inserted 264 pedicle screws in total, and we did not perform registration for each corpus vertebrate in order to shorten the duration of the surgery. We reviewed screw deviation among the items for review using Neo classification with postoperative CT images (1.25 mm). For screw deviation in this case, grade 2 or higher in the Neo classification system was designated as total deviation. Furthermore, we evaluated the registration period per corpus vertebrae, the complications, duration of surgery, blood loss, Cobb angle, and the correction rate.
In terms of screw deviation, 11 (4.2%) of the 264 inserted screws were classified as total deviation. However, there were no neurovascular complications during or after surgery in any cases, and all cases maintained strong internal fixation. In the relationship between the use or nonuse of registration and the deviation, four screws (3.2%) in the corpus vertebrae for which registration was performed and seven screws (5.0%) in the adjacent corpus vertebrae for which registration was not performed had deviated. The duration of registration per corpus vertebrate averaged 4 min and 24 s (58-791 s), but registration also requires a learning curve, so the duration of registration per corpus vertebrae averaged 1 min and 14 s in more recent cases, thus marking a significant shortening.
Segmental pedicle screw fixation are excellent in regard to their fixing and correction force and have been clinically applied even in surgery for scoliosis, but the potential risk of neurovascular complications is unavoidable. The adoption of a navigation system in surgery for scoliosis is useful to increase the safety and certainty of the insertion of pedicle screws.

DOI： 10.1007/s00402-008-0807-3

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Mechanical stretch stimulates integrin alpha V beta 3-mediated collagen expression in human anterior cruciate ligament cells

Tomonori Tetsunaga, Takayuki Furumatsu, Nobuhiro Abe, Keiichiro Nishida, Keiji Naruse, Toshifumi Ozaki

JOURNAL OF BIOMECHANICS 42 ( 13 ) 2097 - 2103 2009.9

Language：English Publisher：ELSEVIER SCI LTD

Biomechanical stimuli have fundamental roles in the maintenance and remodeling of ligaments including collagen gene expressions. Mechanical stretching signals are mainly transduced by cell adhesion molecules such as integrins. However. the relationships between stress-induced collagen expressions and integrin-mediated cellular behaviors are still unclear in anterior cruciate ligament cells. Here, we focused on the stretch-related responses of different cells derived from the ligament-to-bone interface and midsubstance regions of human anterior cruciate ligaments. Chondroblastic interface cells easily lost their potential to produce collagen genes in non-stretched conditions, rather than fibroblastic midsubstance cells. Uni-axial mechanical stretches increased the type I collagen gene expression of interface and midsubstance cells up to 14- and 6-fold levels of each non-stretched control, respectively. Mechanical stretches also activated the stress fiber formation by shifting the distribution of integrin alpha V beta 3 to the peripheral edges in both interface and midsubstance cells. In addition, integrin alpha V beta 3 colocalized with phosphorylated focal adhesion kinase in stretched cells. Functional blocking analyses using anti-integrin antibodies revealed that the stretch-activated collagen gene expressions on fibronectin were dependent on integrin alpha V beta 3-mediated cellular adhesions in the interface and midsubstance cells. These findings suggest that the integrin alpha V beta 3-mediated stretch signal transduction might have a key role to stimulate collagen gene expression in human anterior cruciate ligament, especially in the ligament-to-bone interface. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jbiomech.2009.06.016

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Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice. International journal

Hiroyuki Nakahara, Haruo Misawa, Takahiro Hayashi, Eisaku Kondo, Takeshi Yuasa, Yasuhiro Kubota, Masayuki Seita, Hironobu Kawamoto, Wael A R A Hassan, Reham A R A Hassan, Shahid M Javed, Masato Tanaka, Hirosuke Endo, Hirofumi Noguchi, Shinichi Matsumoto, Katsuyoshi Takata, Yuichi Tashiro, Shuhei Nakaji, Toshifumi Ozaki, Naoya Kobayashi

Transplantation 88 ( 3 ) 346 - 53 2009.8

Language：English

BACKGROUND: Human mesenchymal stem cells (hMSCs) are multipotent stem cells found in the adult bone marrow that have the capacity to differentiate into various mesenchymal cell types. The hMSCs may provide a potential therapy to restore damaged tissues or organs of mesenchymal origin; however, a drawback is their limited life span in vitro. METHODS: We immortalized normal hMSCs with retrovirally transmitted human telomerase reverse transcriptase cDNA. One of the immortalized clones (YKNK-12) was established, and the biological characteristics were investigated in vitro and in vivo. RESULTS: YKNK-12 cells were capable of differentiating adipocytes, osetoblasts, and chondrocytes. Osteogenically differentiated YKNK-12 cells produced significant levels of growth factors BMP4, BMP6, FGF6, FGF7, transforming growth factor-beta1, and transforming growth factor-beta3.. Microcomputer tomography T and soft X-ray assays showed an excellent calvarial bone healing in mice after transplantation of osteogenically differentiated YKNK-12 cells. These cells expressed human-specific osteocalcin and increased the gene expression of runt-related transcription factor 2, alkaline phosphatase, osteocalcin, and osterix in the bone regenerating area. YKNK-12 cell transplant corrected the bone defect without inducing any adverse effects. CONCLUSIONS: We conclude that hMSCs immortalized by transduction with human telomerase reverse transcriptase may provide an unlimited source of cells for therapeutic use in bone regeneration.

DOI： 10.1097/TP.0b013e3181ae5ba2

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Radiological and Clinical Results of Rotational Acetabular Osteotomy Combined with Femoral Intertrochanteric Osteotomy for Avascular Necrosis Following Treatment for Developmental Dysplasia of the Hip

Hiroshi Minagawa, Ayako Aiga, Hirosuke Endo, Shigeru Mitani, Tomonori Tetsunaga, Toshifumi Ozaki

ACTA MEDICA OKAYAMA 63 ( 4 ) 169 - 175 2009.8

Language：English Publisher：OKAYAMA UNIV MED SCHOOL

This retrospective study assessed the radiographic results of 16 patients with avascular necrosis following treatment for developmental dislocation of the hip (DDH) who were subsequently treated between 1991 and 2005 by rotation acetabular osteotomy (RAO) combined with femoral intertrochanteric osteotomy (FIO). Initial treatment was by Pavlik harness, cast fixation, or overhead traction. The parameters that showed consistent improvement were the index of centralization, the index of acetabular coverage, adequate reduction of the greater trochanter, and abductor sufficiency. The combined procedure appears to be effective in cases in which preoperative planning shows a reasonable expectation of congruency and osteoarthritis is limited to the early stages.

DOI： 10.18926/AMO/31814

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