Faculty Profiles - KAMIOKA Hiroshi

Papers - KAMIOKA Hiroshi

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Erratum: Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes (Journal of Bone and Mineral Metabolism (2007) 25, (151-158) DOI: 10.1007/s00774-006-0745-5) Reviewed

Sakhr A. Murshid, Hiroshi Kamioka, Yoshihito Ishihara, Ryoko Ando, Yasuyo Sugawara, Teruko Takano-Yamamoto

Journal of Bone and Mineral Metabolism 25 ( 4 ) 259 2007.7

A case of Stickler syndrome with orthodontic treatment

Hiroshi Kamioka, Yoshihito Ishihara, Daisuke Sawada, Teruko Takano-Yamamoto

Orthodontic Waves 66 ( 2 ) 52 - 59 2007.6

Language：English Publishing type：Research paper (scientific journal)

At the age of 10 years and 10 months, a patient was brought to our office with a chief complaint of dental crowding and narrow maxilla. She had been diagnosed as having Pierre-Robin sequence at birth. She showed a convex profile with Class II molar relationship. The dental relationship was corrected with headgear and functional appliance in phase I orthodontic treatment. Optimal intercuspation of the teeth achieved after edgewise treatment and good facial profile with Class I relationship were obtained. When she started retention at the age of 17 years, she was diagnosed as having Stickler syndrome. Although slight reduction of the mandible was observed after a retention period of 5 years, optimal intercuspation of teeth was maintained. © 2007 Elsevier Ltd and the Japanese Orthodontic Society.

DOI： 10.1016/j.odw.2007.03.002

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Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts

Hiroshi Kamioka, Yoshihito Ishihara, Hans Ris, Sakhr A. Murshid, Yasuyo Sugawara, Teruko Takano-Yamamoto, Soo-Siang Lim

MICROSCOPY AND MICROANALYSIS 13 ( 2 ) 108 - 117 2007.4

Language：English Publishing type：Research paper (scientific journal) Publisher：CAMBRIDGE UNIV PRESS

The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication. In this article, we report successfully isolating primary cultures of osteocytes from chick calvaria, and, using anti-connexin 43 immunocytochemistry, demonstrate gap junction distribution to be comparable to that found in vivo. Next, we demonstrate the functionality of the gap junctions by (1) dye coupling studies that showed the spread of microinjected Lucifer Yellow from osteoblast to osteocyte and between adjacent osteocytes and (2) analysis of fluorescence replacement after photobleaching (FRAP), in which photobleaching of cells loaded with a membrane-permeable dye resulted in rapid recovery of fluorescence into the photobleached osteocyte, within 5 min postbleaching. This FRAP effect did not occur when cells were treated with a gap junction blocker (18 alpha-glycyrrhetinic acid), but replacement of fluorescence into the photobleached cell resumed when it was removed. These studies demonstrate that gap junctions are responsible for intercellular communication between adjacent osteocytes and between osteoblasts and osteocytes. This role is consistent with the ability of osteocytes to respond to and transmit signals over long distances while embedded in a calcified matrix.

DOI： 10.1017/S143192760707016X

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Less response of osteocyte than osteoblast to mechanical force: implication of different focal adheison fometion

Takano-YamamotoT, Kamioka H, Sugawara Y

Interface Oral Health Science 1 ( 1 ) 149 - 158 2007

Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes. Reviewed

Sakhr A Murshid, Hiroshi Kamioka, Yoshihito Ishihara, Ryoko Ando, Yasuyo Sugawara, Teruko Takano-Yamamoto

Journal of bone and mineral metabolism 25 ( 3 ) 151 - 8 2007

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER TOKYO

Cell shape is the most critical determinant of cell function and is potentially influenced by the organization of a cell's cytoskeletal components. It has been reported that three-dimensionally cultured osteoblasts have a morphology that closely resembles that of osteocytes, most notably including formation of processes. We have previously shown the critical differences between cytoskeletal components in osteoblasts and osteocytes in two-dimensional culture. We have now extended that investigation to the cytoskeletal components of 3D-cultured osteoblasts and osteocytes using 3D cultures of the osteoblast cell line, MC3T3-E1, and primary osteocytes grown in collagen gel. Three-dimensional fluorescent image reconstructions for actin, fimbrin, alpha-actinin, myosin, tropomyosin, and microtubules were made using IMARIS software. Actin, fimbrin, alpha-actinin, myosin, and tropomyosin all appeared in the processes of both cell types, but fimbrin and myosin showed differences in their distribution patterns between cell types. Microtubules were limited in distribution to the proximal region of osteocyte processes but extended the entire length of MC3T3-E1 cell processes. Microtubules were essential for the integrity and formation of MC3T3-E1 cell processes, but osteocyte processes were dependent on actin. These results showed that there are significant differences between the actin and microtubule cytoskeletons in the processes of 3D-cultured MC3T3-E1 cells and in the processes of 3D-cultured primary osteocytes. These differences in the cytoskeleton of the processes of 3D-cultured osteoblasts and of osteocyte dendrites suggest that osteoblast processes may have a different functional role than the osteocyte dendritic network.

DOI： 10.1007/s00774-006-0745-5

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alpha(V)beta(3) Integrin ligands enhance volume-sensitive calcium influx in mechanically stretched osteocytes

Akimitsu Miyauchi, Masayuki Gotoh, Hiroshi Kamioka, Kohei Notoya, Hideki Sekiya, Yasuyuki Takagi, Yoshio Yoshimoto, Hitoshi Ishikawa, Kazuo Chihara, Teruko Takano-Yamamoto, Takuo Fujita, Yuko Mikuni-Takagaki

JOURNAL OF BONE AND MINERAL METABOLISM 24 ( 6 ) 498 - 504 2006.11

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER TOKYO

We propose that specific osteocyte-matrix interactions regulate the volume-sensitive calcium influx pathway, which we have shown is mediated by stretch-activated cation channels (SA-Cat) and is essential for the stretch-activated anabolic response in bone. The current study measured the hypotonic swelling-induced increase in cytosolic calcium concentration, [Ca2+](i), in rat osteocytes, and found that cells adherent to different matrices behave differently. Osteopontin and vitronectin, matrix molecules that bind the alpha(V)beta(3) integrin, induced larger responses to the hypotonic swelling than other matrix molecules that bind other integrins. Addition of echistatin, which is a soluble alpha(V)beta(3) ligand, significantly enhanced the hypotonic [Ca2+](i) increase in addition to inducing an immediate increase in [Ca2+](i) by itself. These results strongly support the contention that alpha(V)beta(3) integrin signaling in osteocytes interacts with that in mechanotransduction, which is downstream of SA-Cat.

DOI： 10.1007/s00774-006-0716-x

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Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: Implication of different focal adhesion formation

Hiroshi Kamioka, Yasuyo Sugawara, Sakhr A. Murshid, Yoshihito Ishihara, Tadashi Honjo, Teruko Takano-Yamamoto

JOURNAL OF BONE AND MINERAL RESEARCH 21 ( 7 ) 1012 - 1021 2006.7

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

Introduction: To explore the immediate response to mechanical stress in a bone cell population, we examined flow-induced calcium transients. In addition, the involvement of focal adhesion-related calcium transients in response to fluid flow in the cells was studied.
Materials and Methods: Bone cells were isolated from 16-day-old embryonic chicken calvaria by serial treatment with EDTA and collagenase. Single cells on glass without intercellular connections were subjected to fluid flow, and intracellular calcium concentration was measured using imaging with fluo-3. The identification of cell populations in the same field was performed with a chick osteocyte-specific antibody, OB7.3, and an alkaline phosphatase substrate, ELF-97, for osteoblast identification afterward. Immunofluorescence staining of vinculin was performed to visualize focal adhesions.
Results: The percentage of cells responding to fluid shear stress at 1.2 Pa was 5.5% in osteocytes, 32.4% in osteoblasts, and 45.6% in OB7.3/ELF-97-negative cells. Furthermore, osteoblasts and OB7.3/ELF-97-negative cells were more responsive to 2.4 Pa than 1.2 Pa, whereas osteocytes were less responsive. The elevation of calcium transients over baseline did not show any significant differences in the populations. To elucidate the mechanism accounting for the fact that single osteocytes are less sensitive to fluid shear stress of up to 2.4 Pa than osteoblasts, we studied focal adhesion-related calcium transients. First, we compared focal adhesion formation between osteocytes and osteoblasts and found a larger number of focal adhesions in osteoblasts than in osteocytes. Next, when the cells were pretreated with GRGDS (0.5 mM) before flow treatment, a significant reduction of calcium transients in osteoblasts (18%) was observed, whereas calcium transients in osteocytes were not changed by GRGDS. Control peptide GRGES did not reduce the calcium transients in either cell type. Furthermore, we confirmed that osteoblasts in calvaria showed a marked formation of vinculin plaques in the periphery of the cells. However, osteocytes in calvaria showed faint vinculin plaques only at the base of the processes.
Conclusions: On glass, single osteocytes are less sensitive to fluid shear stress up to 2.4 Pa than osteoblasts. The difference in calcium transients might be related to differences in focal adhesion formation. Shear stress of a higher magnitude or direct deformation may be responsible for the mechanical response of osteocytes in bone.

DOI： 10.1359/JBMR.060408

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Quantitative evaluation of cortical bone thickness with computed tomographic scanning for orthodontic implants

Toru Deguchi, Miho Nasu, Kaoru Murakami, Toshinori Yabuuchi, Hiroshi Kamioka, Teruko Takano-Yamamoto

American Journal of Orthodontics and Dentofacial Orthopedics 129 ( 6 ) 721 - e12 2006.6

Language：English Publishing type：Research paper (scientific journal)

Introduction: The purpose of this study was to quantitatively evaluate cortical bone thickness in various locations in the maxilla and the mandible. In addition, the distances from intercortical bone surface to root surface, and distances between the roots of premolars and molars were also measured to determine the acceptable length and diameter of the miniscrew for anchorage during orthodontic treatment. Methods: Three-dimensional computed tomographic images were reconstructed for 10 patients. Cortical bone thicknesses were measured in the buccal and lingual regions mesial and distal to the first molar, distal to the second molar, and in the premaxillary region at 2 different levels. Differences in cortical bone thickness at 3 angles (30°, 45°, and 90°) were also assessed. Distances of the intercortical bone surface to the root surface and the root proximity were also measured at the above areas. Results: Significantly less cortical bone thickness was observed at the buccal region distal to the second molar compared with other areas in the maxilla. Significantly more cortical bone was observed on the lingual side of the second molar compared with the buccal side. In the mandible, mesial and distal to the second molar, significantly more cortical bone was observed compared with the maxilla. Furthermore, significantly more cortical bone was observed at the anterior nasal spine level than at Point A in the premaxillary region. Cortical bone thickness resulted in approximately 1.5 times as much at 30° compared with 90° Significantly more distance from the intercortical bone surface to the root surface was observed at the lingual region than at the buccal region mesial to the first molar. At the distal of the first mandibular molar, significantly more distance was observed compared to that in the mesial, and also compared with both distal and mesial in the maxillary first molar. There was significantly more distance in root proximity in the mesial area than in distal area at the first molar, and significantly more distance was observed at the occlusal level than at the apical level. Conclusions: These data show that the safest location for placing miniscrews might be mesial or distal to the first molar, and an acceptable size of the miniscrew is less than approximately 1.5 mm in diameter and approximately 6 to 8 mm in length. © 2006 American Association of Orthodontists.

DOI： 10.1016/j.ajodo.2006.02.026

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Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei

H Ichikawa, T Yabuuchi, HW Jin, R Terayama, T Yamaai, T Deguchi, H Kamioka, T Takano-Yamamoto, T Sugimoto

BRAIN RESEARCH 1081 113 - 118 2006.4

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat trigeminal ganglion (TG). The immunoreactivity (IR) was detected in 46% of TG neurons. These neurons were mostly small- or medium-sized (range, 149.7-1246.3 mu m(2); mean +/- SD = 373.4 +/- 151.6 mu m(2)). A double immunofluorescence method also revealed that 54% of BDNF-immunoreactive (IR) neurons were immunoreactive for calcitonin-gene related peptide. in addition, 93% of BDNF-IR TG neurons contained vanilloid receptor subtype 1. However, the co-expression of BDNF and vanilloid receptor 1-like receptor was very rare (less than 1%). In the trigeminal sensory nuclei, laminae II of the medullary dorsal horn was abundant in presumed BDNF-IR axon terminals. Such profiles were also detected in the dorsolateral part of the subnucleus oralis. The retrograde tracing and immunohistochemical methods demonstrated that BDNF-IR was common among cutaneous TG neurons (47%) but not tooth pulp TG neurons (13%). The present study indicates that BDNF-IR TG neurons have unmyelinated axons and project to the superficial medullary dorsal horn. It is likely that BDNF-containing neurons in both the trigeminal and spinal sensory systems have similarities in morphology and function. However, the content of BDNF in TG neurons probably depends on their peripheral targets. BDNF seems to convey nociceptive cutaneous input to the trigeminal sensory nuclei. (c) 2006 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.brainres.2006.01.027

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Implementation and evaluation of the debate-style tutrial study in a third-year dental curriculum in Japan.

Shingaki R, Kamioka H, Irie M, Nishimura F

International Education Journal 2006

English Education Using Original Scientific Papers : A Questionnaire Survey of Fifth Grade Students at Okayama University Dental School

KAMIOKA Hiroshi, MIYAWAKI Shouichi, NASU Miho, TAKANO YAMAMOTO Teruko

22 ( 3 ) 307 - 312 2006

731 Observation of Deformation of Osteocytes in Bone Matrix

ITO Shinichi, TANAKA Mototsugu, ADACHI Taiji, KAMIOKA Hiroshi, YAMAMOTO(TAKANO) Teruko, HOJO Masaki

The proceedings of the JSME annual meeting 2006 261 - 262 2006

Language：Japanese Publisher：The Japan Society of Mechanical Engineers

Osteocyte network system embedded in bone matrix is considered as one of the important elements in the mechanosensory system in bone remodeling. In order to understand the mechanosensory mechanism of osteocytes network, it is essential to evaluate mechanical stimulation to osteocyte network quantitatively as well as to observe biochemical response of osteocyte network to deformation of bone matrix. This study aimed to develop the method to evaluate osteocyte network deformation against bone matrix deformation. Osteocyte network in chick calvarial bone tissue was visualized by immunostaining of cell membrane using OB7.3. Osteocyte network displacement induced by localized deformation to bone matrix using a glass microneedle was observed. Analysis of osteocyte network displacement was fairly successful using the image-correlation method. Applicability of the method newly developed in this study was discussed in combination with biochemical response observation of change in intracellular calcium ion concentration using the fluorescent ratiometry method, in order to investigate the relation between macroscopic deformation of bone matrix and microscopic cellular response.

DOI： 10.1299/jsmemecjo.2006.5.0_261

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732 Relation between Mechanical Stimulation by Localized Deformation and Calcium Signaling Response in Single Isolated Osteocytes

AONUMA Yuki, TANAKA Mototsugu, ADACHI Taiji, KAMIOKA Hiroshi, YAMAMOTO(TAKANO) Teruko, YANO Sumio, HOJO Masaki

The proceedings of the JSME annual meeting 2006 263 - 264 2006

Language：Japanese Publisher：The Japan Society of Mechanical Engineers

The osteocyte is considered as a candidate of the mechanosensory cells in bone tissue. In order to understand the mechanosensory mechanism of osteocytes, it is essential to evaluate mechanical stimulation to osteocytes quantitatively as well as to observe biochemical response of osteocytes to mechanical stimulus. This study aimed to develop the method to evaluate and control mechanical stimulation to osteocytes. Microbeads coated with OB7.3 were attached to chick calvarial isolated osteocytes. Mechanical stimulus applied by pushing microbeads using a glass microneedle was successfully evaluated and controlled. Change in the intracellular Ca^<2+> concentration induced by localized mechanical stimulation by a microneedle was observed using a confocal laser-scanning microscope. Calcium signaling response was up-regulated by mechanical stimulation to proximal base of cell process, while it was not up-regulated by mechanical stimulation to cell body.

DOI： 10.1299/jsmemecjo.2006.5.0_263

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Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy

Y Sugawara, H Kamioka, T Honjo, K Tezuka, T Takano-Yamamoto

BONE 36 ( 5 ) 877 - 883 2005.5

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE INC

Osteocytes are surrounded by hard bone matrix. Therefore, it has not previously been possible to demonstrate the real architecture of the osteocyte network in bone. We previously reported that it is possible to observe osteocytes in bone by labeling the cells with fluorescence and using confocal laser scanning (CLS) microscopy. In this study, we for the first time conducted an extensive analysis of the morphology and morphometry of the three-dimensional (3D) osteocyte structure using three-dimensionally reconstructed fluorescent images. Sixteen-day-old embryonic chick calvariae were stained with fluorescently labeled phalloidin and observed using a confocal laser scanning microscope. Morphometry of osteocytes in the calvaria was analyzed using extensive three-dimensional reconstructing software IMARIS, process length measuring software NEURON TRACER and cell surface area-/cell volume-analyzing software SURPASS. From the IMARIS-derived images, we found that the average of 10 osteocytes is 52.7 &PLUSMN; 5.7 processes, and the point-to-point distance between centers of the osteocytes was 24.1 &PLUSMN; 2.8 μ m. In addition, we could calculate that each osteocyte spans an average of 4180 &PLUSMN; 673 μ m(3) of bone volume. NEURON TRACER showed that the length of osteocyte processes was 0.26 &PLUSMN; 0.02 μ m per 1 μ m(3) bone compartment. In addition, SURPASS indicated that the surface area of osteocytes was 0.36 &PLUSMN; 0.03 μ m(2) per 1 μ m(3) bone compartment and that the volume ratio of osteocyte cell body to bone compartment was 9.42% &PLUSMN; 1.18%. Together, the average total length of the processes, the average surface area, and the average volume of one osteocyte were 1070 &PLUSMN; 145 μ m, 1509 &PLUSMN; 113 μ m(2), and 394 &PLUSMN; 49 μ m(3), respectively. It is possible to reconstruct the real architecture of the osteocyte network and obtain morphometric data from fluorescently labeled osteocytes in chick calvaria. &COPY; 2004 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bone.2004.10.008

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最近7年間に発表された英語原著論文を用いた岡山大学歯学部5年次生への英語教育について.

上岡寛, 宮脇正一, 出口徹, 黒田晋吾, 福永智広, 湊雅直, 菅原康代, 川邉紀章, 荒木佳子, 片山朗, 山本照子

岡山歯学会雑誌 2005

1254 Response of Isolated Osteocyte to Mechanical Stimulus

TANAKA Mototsugu, AONUMA Yuki, ADACHI Taiji, KAMIOKA Hiroshi, TAKANO-YAMAMOTO Teruko, HOJO Masaki

The proceedings of the JSME annual meeting 2005 119 - 120 2005

Language：Japanese Publisher：The Japan Society of Mechanical Engineers

Osteocyte network system embedded in bone matrix is considered as one of the important elements in the mechanosensory mechanism. This study observed the response of an isolated osteocyte to mechanical stimulus, as the first step to clarify the complex mechanisms of mechanosensing and signal transduction of the osteocyte network in living tissue matrix. Change in the intracellular Ca^<2+> concentration induced by mechanical stimulus to the osteocyte cell body was observed, and the sensitivity to the stimulus was compared with that of the osteoblast. In addition, the role of cytoskeletal actin filaments in the mechanosensory mechanism was discussed in terms of its localized distribution in the cell body and processes through the comparison with the osteoblasts.

DOI： 10.1299/jsmemecjo.2005.6.0_119

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Possible roles of Runx1 and Sox9 in incipient intramembranous ossification

T Yamashiro, XP Wang, Z Li, S Oya, T Aberg, T Fukunaga, H Kamioka, NA Speck, T Takano-Yamamoto, Thesleff, I

JOURNAL OF BONE AND MINERAL RESEARCH 19 ( 10 ) 1671 - 1677 2004.10

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC BONE & MINERAL RES

We evaluated the detailed expression patterns of Runx1 and Sox9 in various types of bone formation, and determined whether Runx1 expression was affected by Runx2 deficiency and Runx2 expression by Runx1 deficiency. Our results indicate that both Runx1 and Sox9 are intensely expressed in the future osteogenic cell compartment and in cartilage. The pattern of Runx1 and Sox9 expression suggests that both genes could potentially be involved in incipient intramembranous bone formation during craniofacial development.

DOI： 10.1359/JBMR.04081

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Terminal differentiation of osteoblasts to osteocytes is accompanied by dramatic changes in the distribution of actin-binding proteins

H Kamioka, Y Sugawara, T Honjo, T Yamashiro, T Takano-Yamamoto

JOURNAL OF BONE AND MINERAL RESEARCH 19 ( 3 ) 471 - 478 2004.3

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC BONE & MINERAL RES

Immunofluorescence staining of actin-binding proteins in osteoblasts and osteocytes was performed. alpha-Actinin, myosin, and tropomyosin showed similar organization in both osteoblastic stress fibers and osteocyte processes. However, fimbrin, villin, filamin, and spectrin showed dramatic differences in distribution between osteoblasts and osteocytes. This study suggested that terminal differentiation of osteoblasts to osteocytes is accompanied by highly dramatic changes in the distribution of actin-binding proteins.
Introduction: We previously reported that osteocyte shape is dependent on actin filaments. To analyze the terminal differentiation from osteoblasts to osteocytes, we investigated the actin-binding proteins, which are the control elements in the dynamic organization of the actin cytoskeleton.
Materials and Methods: We used primary chick osteocytes and osteoblasts, the phenotypes of which were confirmed by use of OB7.3, a chick osteocyte-specific monoclonal antibody and by detection of alkaline phosphatase activity, respectively, Immunofluorescence staining was performed for visualizing actin-binding proteins. Furthermore, we applied shear stress at 12 dyns/cm(2) to the cells and compared the changes in fimbrin distribution.
Results: Immunofluorescence staining of fimbrin and alpha-actinin showed their presence in the processes of osteocytes, with especially strong signals of fimbrin at the sites of divarication of the processes. Anti-villin was reactive with the osteocyte cytoplasm but not with the processes. Interestingly, anti-villin immunoreactivity was much stronger in osteocytes than in osteoblasts. Filamin was localized along the stress fibers of osteoblasts but was seen only in those in the proximal base of osteocyte processes. Myosin and tropomyosin were found to have a similar pattern in both stress fibers of osteoblasts and osteocyte processes. The difference in the distribution of anti-spectrin staining was highly dramatic. Osteoblasts immunostained with anti-spectrin showed punctate signals on their cytoplasmic membranes, whereas anti-spectrin in osteocytes detected a filamentous organization; and the spectrin was totally colocalized with actin from the distal portion of the cytoplasmic processes to the cell center. In osteoblasts, shear stress induced recruitment of fimbrin to the end of stress fibers. However, fimbrin in the osteocyte processes did not change its localization.
Conclusion: We found that terminal differentiation of osteoblasts to osteocytes was accompanied by highly dramatic changes in the distribution of actin-binding proteins, changes of which may affect cellular function.

DOI： 10.1359/JBMR.040128

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A surgical orthodontic case of anterior open bite developing during adolescence.

Kamioka H, Honjo T, Takano-Yamamoto T

Orthodontic Waves 2004

岡山大学医学部・歯学部附属病院矯正歯科における平成１５年度歯科医師卒後臨床研修内容

宮本学, 宮脇正一, 出口徹, 藤木辰哉, 福永智広, 黒田晋吾, 坪井佳子, 谷本裕子, 上岡寛, 山城隆, 山本照子

岡山歯学会雑誌 23 ( 2 ) 201 - 205 2004

Language：Japanese Publisher：岡山歯学会

平成15年度岡山大学医学部・歯学部附属病院歯科医師卒後臨床研修のベーシックプログラムに対する矯正歯科の関わりについて総括し,今後の課題について考察した.全人的な視点からさまざまな歯科治療分野に関わる適切で十分な医療情報を収集し,それらに基づいた治療計画を立案する能力を育成することは,総合歯科研修の大変重要な目的で,矯正歯科のような専門的な知識を基にした診断能力の育成も重要である.基本技術の育成を重要視しながら,こういった診断能力を育成していくことは,これからの社会に貢献できる歯科医師の育成にとって必須であることが示唆された

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Papers - KAMIOKA Hiroshi