Faculty Profiles - KAMIOKA Hiroshi

Papers - KAMIOKA Hiroshi

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The Analysis of Gap Junctional Intercellular Communication Among Osteocytes in Chick Calvariae by Fluorescence Recovery After Photobleaching Invited Reviewed

Ziyi Wang, Yoshihito Ishihara, Hiroshi Kamioka

Methods in Molecular Biology 215 - 223 2020

歯科矯正用アンカースクリューによりデンタルディコンペンセーションを図った骨格性III級症例に対する外科的矯正治療 Invited Reviewed

田中智代, 村上隆, 森谷徳文, 松村達志, 飯田征二, 上岡寛

日本顎変形症学会雑誌 29 ( 4 ) 295 - 303 2020

Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity Reviewed

T. Miyazaki, Z. Zhao, Y. Ichihara, D. Yoshino, T. Imamura, K. Sawada, S. Hayano, H. Kamioka, S. Mori, H. Hirata, K. Araki, K. Kawauchi, K. Shigemoto, S. Tanaka, L. F. Bonewald, H. Honda, M. Shinohara, M. Nagao, T. Ogata, I. Harada, Y. Sawada

Science Advances 5 ( 9 ) eaau780 2020

フッ素イオンによるCCNファミリー遺伝子の制御

水川朋美, 西田崇, 明石翔, 堀彩花, 高柴正悟, 上岡寛, 滝川正春, 久保田聡

岡山歯学会雑誌 38 ( 2 ) 85 - 85 2019.12

Role of intracellular Ca2+-based mechanotransduction of human periodontal ligament fibroblasts Reviewed

Ei Hsu, Hlaing E, Ishihara Y, Wang Z, Odagaki N, Kamioka H

FASEB J 33 ( 9 ) 10409 - 10424 2019.9

Screening of key candidate genes and pathways for osteocytes involved in the differential response to different types of mechanical stimulation using a bioinformatics analysis. Reviewed

Ziyi Wang, Yoshihito Ishihara, Takanori Ishikawa, Mitsuhiro Hoshijima, Naoya Odagaki, Ei Ei Hsu Hlaing, Hiroshi Kamioka

Journal of bone and mineral metabolism 37 ( 4 ) 614 - 626 2019.7

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

This study aimed to predict the key genes and pathways that are activated when different types of mechanical loading are applied to osteocytes. mRNA expression datasets (series number of GSE62128 and GSE42874) were obtained from Gene Expression Omnibus database (GEO). High gravity-treated osteocytic MLO-Y4 cell-line samples from GSE62128 (Set1), and fluid flow-treated MLO-Y4 samples from GSE42874 (Set2) were employed. After identifying the differentially expressed genes (DEGs), functional enrichment was performed. The common DEGs between Set1 and Set2 were considered as key DEGs, then a protein-protein interaction (PPI) network was constructed using the minimal nodes from all of the DEGs in Set1 and Set2, which linked most of the key DEGs. Several open source software programs were employed to process and analyze the original data. The bioinformatic results and the biological meaning were validated by in vitro experiments. High gravity and fluid flow induced opposite expression trends in the key DEGs. The hypoxia-related biological process and signaling pathway were the common functional enrichment terms among the DEGs from Set1, Set2 and the PPI network. The expression of almost all the key DEGs (Pdk1, Ccng2, Eno2, Egln1, Higd1a, Slc5a3 and Mxi1) were mechano-sensitive. Eno2 was identified as the hub gene in the PPI network. Eno2 knockdown results in expression changes of some other key DEGs (Pdk1, Mxi1 and Higd1a). Our findings indicated that the hypoxia response might have an important role in the differential responses of osteocytes to the different types of mechanical force.

DOI： 10.1007/s00774-018-0963-7

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Biomimetic mineralization using matrix vesicle nanofragments. Reviewed International journal

Yosuke Kunitomi, Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takuo Kuboki, Takayoshi Nakano, Hiroshi Kamioka, Takuya Matsumoto

Journal of biomedical materials research. Part A 107 ( 5 ) 1021 - 1030 2019.5

Language：English Publishing type：Research paper (scientific journal)

In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021-1030, 2019.

DOI： 10.1002/jbm.a.36618

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スプリントによる下顎位確認後に下顎枝垂直骨切り術を行い偏位側への側方滑走運動が改善した顔面非対称症例 Reviewed

植田紘貴, 岡直毅, 上岡寛

日本顎変形症学会雑誌 29 ( 2 ) 192 - 192 2019.5

岡山大学病院口唇裂・口蓋裂総合治療センター開設後の受診患者の実態調査

植田紘貴, 国富陽介, 中西泰之, 片岡伴記, 村上隆, 木股敬裕, 飯田征二, 上岡寛

日本口蓋裂学会雑誌 44 ( 2 ) 147 - 147 2019.4

Nonsurgical orthodontic treatment of a hypodivergent adult patient with bilateral posterior scissors bite and excessive overjet. Reviewed International journal

Nakamura M, Kawanabe N, Adachi R, Yamashiro T, Kamioka H

Angle Orthodontist 89 ( 2 ) 333 - 349 2019.3

Language：English Publishing type：Research paper (scientific journal)

This report illustrates successful nonsurgical orthodontic treatment of a hypodivergent adult patient with bilateral posterior scissors bite (Brodie bite) and excessive overjet. A 26-year-old woman primarily reported maxillary incisor protrusion. She was diagnosed with Class ll division 1 malocclusion with skeletal Class I, short face, low mandibular plane angle and bilateral posterior scissors bite. A lingual arch with anterior bite block and posterior miniscrews with preadjusted edgewise appliances were used to improve the bilateral scissors bite. After achieving molar occlusion, the maxillary first premolars were extracted, and six miniscrews were used to improve the anterior-posterior and vertical discrepancies. After active treatment for 56 months, the convex facial profile with excessively protruded lips was improved and good interdigitation with ideal incisor relationship was achieved. Additionally, the irregular movements of the incisal path and the bilateral condyles during lateral excursion were improved. At 13 months of retention, a satisfactory facial profile, occlusion, and jaw movements were maintained. The treatment results suggest that miniscrews and fixed bite blocks were effective and efficient to facilitate correction of the bilateral scissors bite, excessive overjet, and vertical relationship correction in this nonsurgical orthodontic treatment.

File： Nakamura(AngleOrthod)2019.pdf

DOI： 10.2319/111617-791.1

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咬合平面傾斜と下顎両側側切歯先天欠如を伴う叢生症例に対し歯科矯正用アンカースクリューを併用し治療を行った1症例 Reviewed

河野加奈, 柳田剛志, 上岡寛

中・四国矯正歯科学会雑誌 1 ( 1 ) 39 - 48 2019

Surgical Orthodontic Correction of a Skeletal Class Ⅲ Case Treated by Dental Decompensation Using Anchor Screws Reviewed

TOMOYO TANAKA, TAKASHI MURAKAMI, NORIFUMI MORITANI, TATSUSHI MATSUMURA, SEIJI IIDA, HIROSHI KAMIOKA

The Japanese Journal of Jaw Deformities 29 ( 4 ) 295 - 303 2019

Differential distribution of microtubules in immature osteocytes in vitro. Reviewed

Murshid S, Takano-Yamamoto T, Kamioka H

Journal of Oral Biosciences 60 ( 4 ) 98 - 101 2018.12

Orthodontic correction of severe Class II malocclusion in a patient with Prader-Willi syndrome. Reviewed International journal

Ishihara Y, Sugawara Y, Ei Hsu, Hlaing E, Nasu M, Kataoka T, Odagaki N, Takano-Yamamoto T, Yamashiro T, Kamioka H

Am J Orthod Dentofacial Orthop. 154 ( 5 ) 718 - 732 2018.11

Language：English Publishing type：Research paper (scientific journal)

Prader-Willi syndrome (PWS) is a complex disorder that affects multiple systems and may cause craniofacial and dentofacial abnormalities. However, there is still a lack of evidence in the literature regarding the progress of orthodontic treatment in patients with PWS. This case report describes the successful orthodontic treatment of a patient with PWS. A girl, 9 years 0 months of age, who had been diagnosed with PWS had protruding maxillary incisors and a convex profile. Her malocclusion was due to the posteriorly positioned mandible. Screening tests for sleep apnea syndrome showed that she had sleep-disordered breathing, including obstructive sleep apnea and bruxism. We also observed an excessive overjet of 10.0 mm, a deep overbite of 6.8 mm, and the congenital absence of the mandibular second premolars. The patient was diagnosed with an Angle Class II malocclusion and a skeletal Class II jaw-base relationship with a deep overbite. Functional appliance therapy with mandibular advancement, which can enlarge the upper airway and increase the upper airspace, was performed to prevent further deterioration of the patient's obstructive sleep apnea. An acceptable occlusion with a proper facial profile and functional excursion were achieved without interference after comprehensive 2-stage treatment that incorporated orthodontic therapy for the patient's excessive overjet and deep overbite. The resulting occlusion was stable, and the occlusal force and the contact area gradually increased over a 2-year retention period. These results suggest that orthodontic treatment offers the opportunity to greatly improve the health and quality of life of people with PWS.

DOI： 10.1016/j.ajodo.2017.05.040

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Role of osteocyte-PDL crosstalk in tooth movement via SOST/Sclerostin Reviewed

Odagaki N, Ishihara Y, Wang Z, Ei Hsu, Hlaing Ei, Nakamura M, Hoshimima M, Hayano S, Kawanabe N, Kamioka H

Journal of Dental Research 97 ( 12 ) 1374 - 1382 2018.11

Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone. Reviewed

Tomoyo Tanaka, Mitsuhiro Hoshijima, Junko Sunaga, Takashi Nishida, Mana Hashimoto, Naoya Odagaki, Ryuta Osumi, Taiji Aadachi, Hiroshi Kamioka

Journal of bone and mineral metabolism 36 ( 5 ) 519 - 528 2018.9

Language：English Publishing type：Research paper (scientific journal)

Osteocytes form a three-dimensional (3D) cellular network within the mineralized bone matrix. The cellular network has important roles in mechanosensation and mechanotransduction related to bone homeostasis. We visualized the embedded osteocyte network in chick calvariae and observed the flow-induced Ca2+ signaling in osteocytes using 3D time-lapse imaging. In response to the flow, intracellular Ca2+ ([Ca2+]i) significantly increased in developmentally mature osteocytes in comparison with young osteocytes in the bone matrix. To investigate the differences in response between young and developmentally mature osteocytes in detail, we evaluated the expression of osteocyte-related genes using the osteocyte-like cell line MLO-Y4, which was 3D-cultured within type I collagen gels. We found that the c-Fos, Cx43, Panx3, Col1a1, and OCN mRNA levels significantly increased on day 15 in comparison with day 7. These findings indicate that developmentally mature osteocytes are more responsive to mechanical stress than young osteocytes and have important functions in bone formation and remodeling.

DOI： 10.1007/s00774-017-0868-x

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Human gingival fibroblast feeder cells promote maturation of induced pluripotent stem cells into cardiomyocytes. Reviewed

Matsuda Y, Takahashi K, Kamioka H, Naruse K

Biochem Biophys Res Commun. 503 ( 3 ) 1798 - 1804 2018.9

著しい骨格性下顎前突および多数歯欠如を伴う色素失調症患者に対して外科的矯正治療および補綴処置を行った一例 Reviewed

石本和也, 柳田剛志, 川邉紀章, 上岡寛

中・四国矯正歯科学会雑誌 30 ( 1 ) 23 - 32 2018.8

Role of the inferior alveolar nerve in rodent lower incisor stem cells Reviewed

Hayano S, Fukui Y, Kawanabe N, Nakamura M, Ishihara Y, Kamioka H

Journal of Dental Research 97 ( 8 ) 954 - 961 2018.7

Physiological role of urothelial cancer-associated one long noncoding RNA in human skeletogenic cell differentiation. Reviewed International journal

Takanori Ishikawa, Takashi Nishida, Mitsuaki Ono, Takeshi Takarada, Ha Thi Nguyen, Shinnosuke Kurihara, Takayuki Furumatsu, Yurika Murase, Masaharu Takigawa, Toshitaka Oohashi, Hiroshi Kamioka, Satoshi Kubota

Journal of Cellular Physiology 233 ( 6 ) 4825 - 4840 2018.6

Language：English Publishing type：Research paper (scientific journal)

A vast number of long-noncoding RNAs (lncRNA) are found expressed in human cells, which RNAs have been developed along with human evolution. However, the physiological functions of these lncRNAs remain mostly unknown. In the present study, we for the first time uncovered the fact that one of such lncRNAs plays a significant role in the differentiation of chondrocytes and, possibly, of osteoblasts differentiated from mesenchymal stem cells, which cells eventually construct the human skeleton. The urothelial cancer-associated 1 (UCA1) lncRNA is known to be associated with several human malignancies. Firstly, we confirmed that UCA1 was expressed in normal human chondrocytes, as well as in a human chondrocytic cell line; whereas it was not detected in human bone marrow mesenchymal stem cells (hBMSCs). Of note, although UCA1 expression was undetectable in hBMSCs, it was markedly induced along with the differentiation toward chondrocytes, suggesting its critical role in chondrogenesis. Consistent with this finding, silencing of the UCA1 gene significantly repressed the expression of chondrogenic genes in human chondrocytic cells. UCA1 gene silencing and hyper-expression also had a significant impact on the osteoblastic phenotype in a human cell line. Finally, forced expression of UCA1 in a murine chondrocyte precursor, which did not possess a UCA1 gene, overdrove its differentiation into chondrocytes. These results indicate a physiological and important role of this lncRNA in the skeletal development of humans, who require more sustained endochondral ossification and osteogenesis than do smaller vertebrates.

DOI： 10.1002/jcp.26285

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