Faculty Profiles - KAMIOKA Hiroshi

Assessment of undergraduate orthodontic education : a correlation between written examination, laboratory work, and Objective Structured Clinical Examination (OSCE) of medical interviews :

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MIYAWAKI Shouichi, DEGUCHI Toru, KAMIOKA Hiroshi, FUJIKI Tatsuya, IMAI Mikako, YOSHIDA Toshiko, TAKANO-YAMAMOTO Teruko

Orthodontic waves : journal of the Japanese Orthodontic Society = Orthodontic waves : journal of the Japanese Orthodontic Society 63 ( 3 ) 71 - 75 2004

Language：English

The necessity for a revolution in medical and dental undergraduate education assessment has received considerable attention in recent years. In the case of orthodontic undergraduate curriculum, not only the traditional lecturebased learning or laboratory work, but also the need for specific education to improve communication skills with patients has been emphasized. However, there have not been any studies reporting the correlation between the assessments such as traditional written examination, laboratory work, and medical interviews of the undergraduate knowledge levels. In the present study, we assessed the correlation of these various educational methods. Data were collected from 51 undergraduate students that had taken the same lecture, laboratory work, and Objective Structured Clinical Examination (OSCE) of medical interviews. Statistical analysis was performed using Spearman's correlation coefficient to assess the correlation between these educational methods. Prior to the written examinations (10 questions), undergraduate students took 32 (48 hours) sessions taught by 3 orthodontic faculty members. Those 3 orthodontic faculty members prepared questions from the lectures they had given, and scored the examinations. There were of 6 (27 hours) laboratory work taught by four orthodontic faculty members. Four orthodontic appliances were evaluated by a single orthodontic faculty member. The clinical interview and the lecture were conducted by 2 orthodontic faculty member 2 times (3 hours). Assessment of the OSCE was conducted by 8 well-trained and calibrated dental faculty members. Two faculty members and one student (as a simulated and standardized patient, i. e., SP) evaluated each undergraduate student. Assessment of content and process areas of the OSCE was performed by 2 faculty members. The total examination time was 6 minutes. As a result, the written examination score positively correlated with all other variables excluding the process area. This was the same in the results of OSCE. There was no correlation between laboratory work and either the process area or the total evaluation results of the OSCE. In conclusion, basic scientific knowledge of orthodontics helps in laboratory work and communication skills. However, specific knowledge and skills are required in the process area of conducting a medical interview with patients.

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Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=198645

岡山大学歯学部附属病院矯正科における週1回の診療時間の延長が新患数ならびに矯正治療を開始した患者数に及ぼす影響

山本照子, 宮脇正一, 坪井佳子, 谷本裕子, 山城隆, 上岡寛, 宮本学, 倉谷豪, 藤木辰哉, 黒田晋吾, 出口徹, 藤山光治, 井上雅秀, 大矢伸治, 福永智広, 森重靖子, 本城正, 荒木佳子, 片山朗, 菅原康代, 石光哲郎, 竹下信郎, 湊雅直, 酒井雄一, 藤井昭仁, 藪内利憲

岡山歯学会雑誌 22 ( 1 ) 215 - 218 2003.6

Language：Japanese Publisher：岡山歯学会

平成14年2月より,矯正科において毎週水曜日の診療時間を2時間延長した.診療時間延長開始以降1年間の矯正科新患数は316名で,平成13年度は12年度とほぼ同数であったが,14年度は13年度に比較して11%増加した.14年度の矯正治療開始患者数は201名で,平成12年度は160名,13年度は177名であり,前年度に比較して14%増加した.診療時間延長は患者サービスに多大な貢献をしたことが示された

骨格性下顎前突症にRigid External Distraction systemを適用した仮骨延長の一症例

大矢伸治, 山下和夫, 上岡寛, 山本照子, 香川智正, 三島克章, 菅原利夫

日本顎変形症学会雑誌 11 ( 2 ) 153 - 153 2001.8

Mechanical stimulation induces CTGF expression in rat osteocytes Reviewed

T. Yamashiro, T. Fukunaga, N. Kobashi, H. Kamioka, T. Nakanisni, M. Takigawa, T. Takano-Yamamoto

Journal of Dental Research 80 ( 2 ) 461 - 465 2001

Language：English Publishing type：Research paper (scientific journal) Publisher：SAGE Publications Inc.

Connective tissue growth factor (CTGF), which is encoded by an immediate early gene and a member of the CCN family, has been shown to be expressed in osteoblasts, fibroblasts, and chondrocytes. Although CTGF is expressed in bone and cartilage tissues, we tested the hypothesis that CTGF is regulated in mechanotransduction. In the alveolar bone during experimental tooth movement, CTGF mRNA was expressed in osteoblasts and in osteocytes localized around the periodontal ligament under control conditions. Interestingly, 12 hrs after the start of experimental tooth movement, the expression of CTGF mRNA in osteocytes and osteoblasts became more intense around the periodontal ligament, and the intense expression of CTGF extended to osteocytes situated deep in alveolar bone matrix apart from periodontal ligament in both tension and compression sides. Our present findings indicate that CTGF could play a role in regulation of osteocyte function during the mechanical stimulation of bone.

DOI： 10.1177/00220345010800021201

A three-dimensional distribution of osteocyte processes revealed by the combination of confocal laser scanning microscopy and differential interference contrast microscopy Reviewed

H. Kamioka, T. Honjo, T. Takano-Yamamoto

Bone 28 ( 2 ) 145 - 149 2001

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Osteocytes are the most numerous cells in bone, embedded within the mineralized bone matrix. Their slender cytoplasmic processes form a complex intercellular network. In addition, these processes are thought to be important structures in the response to mechanical stress. This study provides an extensive analysis of the three-dimensional structure of the osteocyte and its processes in 16-day-old embryonic chick calvariae, based on nondestructive subsurface histotomography using both confocal laser scanning (CLS) microscopy and differential interference contrast (DIC) microscopy. OB7.3, a chicken osteocyte-specific monoclonal antibody, and Texas Red-X-conjugated phalloidin were used to confirm the osteocyte phenotype and to identify whole cells in the calvariae, respectively. Serial CLS images revealed morphological changes in bone cells up to 20 μm in depth. Osteocytes had widely spread their processes into the osteoblast layer, and we found for the first time that some of these processes had elongated to the vascular-facing surface of the osteoblast layer. Furthermore, stereotype images reconstructed from CLS images could show the three-dimensional distribution of these processes. Using the stereopair image, we could evaluate the frequency of processes between osteocytes and osteoblasts. Complementation of DIC microscopy revealed canaliculi and lacunae with high contrast. The distributional pattern of canaliculi generally coincided with that of the osteocyte processes. We consider that the combination method of CLS microscopy and DIC microscopy using a laser scanning microscope is a very useful new technical approach for investigating osteocytes in bone. Copyright © 2001 Elsevier Science Inc.

DOI： 10.1016/S8756-3282(00)00421-X

Osteocyte Shape Is Dependent on Actin Filaments and Osteocyte Processes Are Unique Actin-Rich Projections Reviewed

Kayo Tanaka-Kamioka, Hiroshi Kamioka, Hans Ris, Soo-Siang Lim

Journal of Bone and Mineral Research 13 ( 10 ) 1555 - 1568 1998.10

Extracellular calcium causes the release of calcium from intracellular stores in chick osteocytes Reviewed

Hiroshi Kamioka, Yoshiki Miki, Koji Sumitani, Kahori Tagami, Kunihiro Terai, Kazuo Hosoi, Terushige Kawata

Biochemical and Biophysical Research Communications 212 ( 2 ) 692 - 696 1995.7

Language：English Publishing type：Research paper (scientific journal)

We have recently demonstrated that a rise in the extracellular divalent cation concentration induces a rapid elevation of cytosolic calcium in chick osteocytes. Here, we demonstrate that cytosolic calcium elevation that occurs in osteocytes on exposure to elevated extracellular calcium is independent of membrane voltage and is insensitive to modulation by organic calcium channel modulators, namely, BAY K 8644, nicardipine, and nifedipine. However, the calcium elevation was sensitive to modulation by an intracellular calcium antagonist, TMB-8, suggesting that the cytosolic calcium elevation was due to mobilization of this cation from an intracellular store. © 1995 Academic Press, Inc.

DOI： 10.1006/bbrc.1995.2024

Effects of transforming growth factor-β1 on formation and activation of osteoclasts Reviewed

Hiroshi Kamioka, Keishi Ishikawa, Kayo Tanaka, Takuya Sato, Ken-Ichi Tezuka, Kenji Hiura, Koji Sumitani, Yoshiyuki Hakeda, Terushige Kawata, Masayoshi Kumegawa

Journal of Bone and Mineral Metabolism 13 ( 1 ) 3 - 9 1995.3

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer-Verlag

Transforming growth factor-β1 (TGF-β1) has biological functions in various types of cells. However, its roles in the regulation of osteoclast formation and function are unclear. To examine them, we employed a culture system in which unfractionated cells obtained from long bones of 13-day-old mice were cultured on a dentine slice. We found that TGF-β1 has a potent inhibitory effect on osteoclastic bone resorption at a dose of 0.2-5 ng/ml. By electron microscopy the osteoclasts appeared to have fewer mitochondria and ruffled borders than those in control cultures. But in the presence of 1,25-dihydroxyvitamin D3, [1,25-(OH)2D3], TGF-β1 at a dose of 0.2-1 ng/ml stimulated the formation of osteoclasts from unfractionated bone cell cultures in which preexistent osteoclasts had degenerated. Thus, using stromal cell-free he-mopoietic blast cells, we examined the direct action of TGF-β1 on osteoclast precursors. Although TGF-β1 inhibited tartrate-resistant acid phosphatase-positive (TRAP) multinucleate cell (MNC) formation induced by 1,25-(OH)2D3, the conditioned medium (CM) of TGF-β1-treated MC3T3-E1 cells stimulated such formation. These results suggest that TGF-β1 inhibits osteoclastic bone resorption but stimulates osteoclast formation via the action of factor(s) produced by TGF-β1-treated osteoblasts in the presence of 1,25-(OH)2D3. © 1995 Springer-Verlag.

DOI： 10.1007/BF01771798

Divalent cations elevate cytosolic calcium of chick osteocytes Reviewed

Hiroshi Kamioka, Koji Sumitani, Kahori Tagami, Yoshiki Miki, Kunihiro Terai, Yoshiyuki Hakeda, Masayoshi Kumegawa, Terushige Kawata

Biochemical and Biophysical Research Communications 204 ( 2 ) 519 - 524 1994.10

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Though it is proposed that osteocytes have several functions in bone metabolism, only limited definite information including results of calcium regulation has been forthcoming. We demonstrate here for the first time that the concentration of divalent cations, such as calcium, nickel and cadmium, directly regulates the cytosolic calcium concentration in osteocytes. © 1994 Academic Press. All rights reserved.

DOI： 10.1006/bbrc.1994.2490

The mechanisms and regulation of procathepsin L secretion from osteoclasts in bone resorption Reviewed

Kahori Tagami, Hisao Kakegawa, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Brigita Lenarcič, Vito Turk, Nobuhiko Katunuma

FEBS Letters 342 ( 3 ) 308 - 312 1994.4

Language：English Publishing type：Research paper (scientific journal)

The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition ofcalcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes. © 1994.

DOI： 10.1016/0014-5793(94)80522-9

Molecular cloning of a possible cysteine proteinase predominantly expressed in osteoclasts Reviewed

Ken-Ichi Tezuka, Yoko Tezuka, Akiko Maejima, Takuya Sato, Ken Nemoto, Hiroshi Kamioka, Yoshiyuki Hakeda, Masayoshi Kumegawa

Journal of Biological Chemistry 269 ( 2 ) 1106 - 1109 1994.1

Language：English Publishing type：Research paper (scientific journal)

Osteoclasts are multinucleate giant cells playing key roles in bone resorption. These cells solubilize mineralized bone matrix by means of acid and protease action
however, the precise mechanism of this process is not well known. Recently, we succeeded in the isolation of pure osteoclasts from rabbit bones and constructed a cDNA library. Using a differential screening procedure, two genes expressed predominantly in osteoclasts compared with spleen cells were isolated (Tezuka, K., Sato, T., Kamioka, H., Nijweide, P. J., Tanaka, K., Matsuo, T., Ohta, M., Kurihara, N., Hakeda, Y., and Kumegawa, M. (1992) Biochem. Biophys. Res. Commun. 186, 911-917). One of them, OC-2, was found to encode a possible cysteine proteinase structurally related to cathepsins L and S. By in situ hybridization, OC-2 was confirmed to be expressed in osteoclasts in vivo. By Northern blot analysis, OC-2 was highly and preferentially expressed in osteoclasts compared with other tissues such as kidney, liver, spleen, and lung. The predominant expression of OC-2 in osteoclasts may suggest that OC-2 encodes a protein, possibly a cysteine proteinase, that plays an important role in osteoclastic bone resorption.

A simple method of isolation of rabbit osteoclasts Reviewed

Sato T, Kamioka H, Nemoto K, Tezuka K, Tanaka K, Sugimoto T, Kaji H, Miyauchi A, Yoshizawa K, Yamaguchi Y, Hakeda Y, Kumegawa M

Dentistry in Japan 31 3 - 8 1994.1

Participation of cathepsin L on bone resorption Reviewed

Hisao Kakegawa, Takeshi Nikawa, Kahori Tagami, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Marinka Drobnič-Kosorok, Brigita Lenarčič, Vito Turk, Nobuhiko Katunuma

FEBS Letters 321 ( 2-3 ) 247 - 250 1993.4

Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation. © 1993.

DOI： 10.1016/0014-5793(93)80118-E

Identification of osteopontin in isolated rabbit osteoclasts Reviewed

Ken-ichi Tezuka, Takuya Sato, Hiroshi Kamioka, Peter J. Nijweide, Kayo Tanaka, Tetsu Matsuo, Mitsue Ohta, Noriyoshi Kurihara, Yoshiyuki Hakeda, Masayoshi Kumegawa

Biochemical and Biophysical Research Communications 186 ( 2 ) 911 - 917 1992.7

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Bone remodeling is a complex process coupling bone formation and resorption. Osteoblasts, the bone-forming cells, are known to produce various bone matrix proteins and cytokines
however, little is known about protein factors produced by osteoclasts or bone-resorbing cells. A method utilizing the high affinity of osteoclasts for tissue culture dishes was developed to isolate a large number of pure osteoclasts from rabbit long bones. A cDNA library was then constructed from these isolated osteoclasts, and differential cDNA screening was performed between osteoclasts and spleen cells. Two clones representing osteoclast-specific clones, named OC-1 and OC-2, were isolated. By Northern blot analysis, OC-1 was expressed in osteoclasts and in kidneys, whereas OC-2 was specific for osteoclasts. OC-1 was found to encode osteopontin from its nucleotide sequence, and therefore, osteopontin may have other functions for osteoclastic bone resorption besides osteoclast attachment to bone. © 1992.

DOI： 10.1016/0006-291X(92)90832-6