Faculty Profiles - KAMIOKA Hiroshi

Papers - KAMIOKA Hiroshi

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上顎骨骨切り術における翼突上顎縫合の異常骨折に関する形態計測学的検討

小畑協一, 伊原木聰一郎, 上岡寛

日本骨形態計測学会雑誌 32 ( 1 ) S199 - S199 2022.5

多数歯欠損を伴う高齢期の下顎前突症患者に対する外科的矯正治療を伴った包括的歯科治療の1例 Reviewed

河野加奈, 中村政裕, 吉岡徳枝, 川邉紀章, 西山明慶, 佐々木朗, 上岡寛

日本顎変形症学会雑誌 32 ( 1 ) 48 - 59 2022.4

Authorship：Last author,　Corresponding author Language：Japanese Publisher：(NPO)日本顎変形症学会

症例は65歳女性で、顎変形症の治療が保険適用となったことを知り、加療を希望し当院口腔外科を受診後、当科を紹介により受診した。機能性反対咬合および下顎骨の右方偏位を伴う下顎前突症と診断し、骨格性の改善を図るため外科的矯正治療を選択した。歯系では下顎骨の後方移動量を確保するため、術前矯正治療にて下顎前歯部に歯科矯正用アンカースクリューを埋入し、小臼歯の近心移動により空隙を閉鎖するとともに前歯部の唇側傾斜および圧下を図ることとした。上顎歯列は補綴処理による咬合獲得を行い、下顎右側臼歯部においても補綴処置による咬合獲得を行った。軟組織ではオトガイを後退させることでEラインに対する上下口唇の後退を改善し、下顎臼歯部が欠損していたため術後の安定性を考慮して下顎枝矢状分割術を選択した。

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Other Link： https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2022&ichushi_jid=J03047&link_issn=&doc_id=20220510230006&doc_link_id=%2Fdh5jawdf%2F2022%2F003201%2F007%2F0048-0059%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdh5jawdf%2F2022%2F003201%2F007%2F0048-0059%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

A morphometric analysis of the osteocyte canaliculus using applied automatic semantic segmentation by machine learning Reviewed

Kaori Tabata, Mana Hashimoto, Haruka Takahashi, Ziyi Wang, Noriyuki Nagaoka, Toru Hara, Hiroshi Kamioka

Journal of Bone and Mineral Metabolism 40 ( 4 ) 571 - 580 2022.3

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

INTRODUCTION: Osteocytes play a role as mechanosensory cells by sensing flow-induced mechanical stimuli applied on their cell processes. High-resolution imaging of osteocyte processes and the canalicular wall are necessary for the analysis of this mechanosensing mechanism. Focused ion beam-scanning electron microscopy (FIB-SEM) enabled the visualization of the structure at the nanometer scale with thousands of serial-section SEM images. We applied machine learning for the automatic semantic segmentation of osteocyte processes and canalicular wall and performed a morphometric analysis using three-dimensionally reconstructed images. MATERIALS AND METHODS: Six-week-old-mice femur were used. Osteocyte processes and canaliculi were observed at a resolution of 2 nm/voxel in a 4 × 4 μm region with 2000 serial-section SEM images. Machine learning was used for automatic semantic segmentation of the osteocyte processes and canaliculi from serial-section SEM images. The results of semantic segmentation were evaluated using the dice similarity coefficient (DSC). The segmented data were reconstructed to create three-dimensional images and a morphological analysis was performed. RESULTS: The DSC was > 83%. Using the segmented data, a three-dimensional image of approximately 3.5 μm in length was reconstructed. The morphometric analysis revealed that the median osteocyte process diameter was 73.8 ± 18.0 nm, and the median pericellular fluid space around the osteocyte process was 40.0 ± 17.5 nm. CONCLUSION: We used machine learning for the semantic segmentation of osteocyte processes and canalicular wall for the first time, and performed a morphological analysis using three-dimensionally reconstructed images.

DOI： 10.1007/s00774-022-01321-x

PubMed

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Other Link： https://link.springer.com/article/10.1007/s00774-022-01321-x/fulltext.html

Improvement of anterior disc displacement on the mandibular deviated side after intraoral vertical ramus osteotomy in a patient with facial asymmetry: a case report Reviewed

Hirotaka Ueda, Naoki Oka, Tsuyoshi Shimo, Akira Sasaki, Takashi Yamashiro, Hiroshi Kamioka

Clinical and Investigative Orthodontics 81 ( 1 ) 58 - 67 2022.1

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Informa UK Limited

Purpose We present the orthognathic treatment of an adult skeletal Class III patient with facial asymmetry, mandibular rightward deviation, and anterior disc displacement without reduction (ADDwoR) at the right temporomandibular joint (TMJ) by intraoral vertical ramus osteotomy (IVRO). Materials and methods The patient was a 23-year-old man with complaints of mandibular deviation and crowded lower anterior teeth, resulting in facial asymmetry. The maxillary position was normal with protrusion and rightward deviation of the mandible. There was no cant of the maxilla. He experienced pain in the right TMJ during mastication, and Magnetic resonance imaging (MRI) revealed an ADDwoR on the right side. The patient was diagnosed with Class III malocclusion, skeletal Class III prognathism with mandibular deviation, and ADDwoR on the right side. Orthognathic surgery was proposed for jaw deformity, and IVRO was performed to correct mandibular deviation. Results One year and 2 months after treatment onset, IVRO was performed with differential setback: 2 mm on the right and 8 mm on the left side of the mandible. The midline of the lower dentition was rotated by 6 mm to coincide with the facial midline. Symptoms of temporomandibular disorders were not observed post-operatively. Active-treatment period was for 31 months. MRI findings showed improvement in anterior disc displacement on the right side during the post-retention. Conclusion In the case of facial asymmetry with anterior disc displacement on the mandibular deviated side, IVRO was suggested to have a potential effect on the positional relationship between the mandibular head and temporomandibular disc.

DOI： 10.1080/27705781.2022.2044700

Web of Science

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High-resolution image-based simulation reveals membrane strain concentration on osteocyte processes caused by tethering elements Reviewed

Yuka Yokoyama, Yoshitaka Kameo, Hiroshi Kamioka, Taiji Adachi

Biomechanics and Modeling in Mechanobiology 20 ( 6 ) 2353 - 2360 2021.12

Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

<title>Abstract</title>Osteocytes are vital for regulating bone remodeling by sensing the flow-induced mechanical stimuli applied to their cell processes. In this mechanosensing mechanism, tethering elements (TEs) connecting the osteocyte process with the canalicular wall potentially amplify the strain on the osteocyte processes. The ultrastructure of the osteocyte processes and canaliculi can be visualized at a nanometer scale using high-resolution imaging via ultra-high voltage electron microscopy (UHVEM). Moreover, the irregular shapes of the osteocyte processes and the canaliculi, including the TEs in the canalicular space, should considerably influence the mechanical stimuli applied to the osteocytes. This study aims to characterize the roles of the ultrastructure of osteocyte processes and canaliculi in the mechanism of osteocyte mechanosensing. Thus, we constructed a high-resolution image-based model of an osteocyte process and a canaliculus using UHVEM tomography and investigated the distribution and magnitude of flow-induced local strain on the osteocyte process by performing fluid–structure interaction simulation. The analysis results reveal that local strain concentration in the osteocyte process was induced by a small number of TEs with high tension, which were inclined depending on the irregular shapes of osteocyte processes and canaliculi. Therefore, this study could provide meaningful insights into the effect of ultrastructure of osteocyte processes and canaliculi on the osteocyte mechanosensing mechanism.

DOI： 10.1007/s10237-021-01511-y

Web of Science

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Other Link： https://link.springer.com/article/10.1007/s10237-021-01511-y/fulltext.html

Investigation of the molecular causes underlying physical abnormalities in Diamond‐Blackfan anemia patients with RPL5 haploinsufficiency Reviewed International journal

Yuko Fukui, Satoru Hayano, Noriaki Kawanabe, Ziyi Wang, Akira Shimada, Megumu K. Saito, Isao Asaka, Hiroshi Kamioka

Pathology International 71 ( 12 ) 803 - 813 2021.12

Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Diamond-Blackfan anemia (DBA) is a genetic disorder caused by mutations in genes encoding ribosomal proteins and characterized by erythroid aplasia and various physical abnormalities. Although accumulating evidence suggests that defective ribosome biogenesis leads to p53-mediated apoptosis in erythroid progenitor cells, little is known regarding the underlying causes of the physical abnormalities. In this study, we established induced pluripotent stem cells from a DBA patient with RPL5 haploinsufficiency. These cells retained the ability to differentiate into osteoblasts and chondrocytes. However, RPL5 haploinsufficiency impaired the production of mucins and increased apoptosis in differentiated chondrocytes. Increased expression of the pro-apoptotic genes BAX and CASP9 further indicated that RPL5 haploinsufficiency triggered p53-mediated apoptosis in chondrocytes. Murine double minute 2 (MDM2), the primary negative regulator of p53, plays a crucial role in erythroid aplasia in DBA patient. We found the phosphorylation level of MDM2 was significantly decreased in RPL5 haploinsufficient chondrocytes. In stark contrast, we found no evidence that RPL5 haploinsufficiency impaired osteogenesis. Collectively, our data support a model in which RPL5 haploinsufficiency specifically induces p53-mediated apoptosis in chondrocytes through MDM2 inhibition, which leads to physical abnormalities in DBA patients.

DOI： 10.1111/pin.13168

PubMed

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pin.13168

Roles for B[a]P and FICZ in subchondral bone metabolism and experimental temporomandibular joint osteoarthritis via the AhR/Cyp1a1 signaling axis Reviewed

Yuri Yoshikawa, Takashi Izawa, Yusaku Hamada, Hiroko Takenaga, Ziyi Wang, Naozumi Ishimaru, Hiroshi Kamioka

Scientific Reports 11 ( 1 ) 2021.12

Authorship：Last author Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

<title>Abstract</title>Bone loss due to smoking represents a major risk factor for fractures and bone osteoporosis. Signaling through the aryl hydrocarbon receptor (AhR) and its ligands contributes to both bone homeostasis and inflammatory diseases. It remains unclear whether the same AhR signaling axis affects the temporomandibular joint (TMJ). The aim of this study was to investigate possible mechanisms which mediate bone loss in the TMJ due to smoking. In particular, whether benzo[<italic>a</italic>]pyrene (B[<italic>a</italic>]P), a carcinogen of tobacco smoke, induces expression of the AhR target gene, Cyp1a1, in mandibular condyles. Possible functions of an endogenous ligand of FICZ, were also investigated in a TMJ-osteoarthritis (OA) mouse model. B[<italic>a</italic>]P was administered orally to wild-type and <italic>AhR</italic>^−/− mice and bone metabolism was subsequently examined. TMJ-OA was induced in wild-type mice with forceful opening of the mouth. Therapeutic functions of FICZ were detected with μCT and histology. Exposure to B[<italic>a</italic>]P accelerated bone loss in the mandibular subchondral bone. This bone loss manifested with osteoclastic bone resorption and upregulated expression of Cyp1a1 in an <italic>AhR</italic>-dependent manner. In a mouse model of TMJ-OA, FICZ exhibited a dose-dependent rescue of mandibular subchondral bone loss by repressing osteoclast activity. Meanwhile, in vitro, pre-treatment with FICZ reduced RANKL-mediated osteoclastogenesis. B[<italic>a</italic>]P regulates mandibular subchondral bone metabolism via the Cyp1a1. The AhR ligand, FICZ, can prevent TMJ-OA by regulating osteoclast differentiation.

DOI： 10.1038/s41598-021-94470-4

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Other Link： http://www.nature.com/articles/s41598-021-94470-4

岡山大学病院矯正歯科における永久歯先天性欠如の歯種別発生率の調査 Reviewed

太田明美, 星島光博, 中西泰之, 植田紘貴, 早野暁, 上岡寛

岡山歯学会雑誌 40 ( 2 ) 19 - 26 2021.12

Authorship：Lead author Language：Japanese Publisher：岡山歯学会

9年間の矯正歯科治療患者2079名(男性682名、女性1397名、平均年齢20歳1ヵ月、中央値18歳3ヵ月)を対象に、永久歯先天性欠如(先欠歯)発生率を発生部位、歯種別に検討した。その結果、先欠歯発生率は284/2079名(13.7%)で、上顎での発生率が高かった。また、口唇口蓋裂(CLCP)患者や特定疾患患者では他の患者と比較して高い先欠歯発生率を認め、CLCP患者は上顎側切歯、特定疾患患者は上顎第二大臼歯の欠如が特徴的であった。

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O‐GlcNAcylation drives calcium signaling toward osteoblast differentiation: A bioinformatics‐oriented study Reviewed International journal

Yao Weng, Ziyi Wang, Yoko Fukuhara, Airi Tanai, Mika Ikegame, Daisuke Yamada, Takeshi Takarada, Takashi Izawa, Satoru Hayano, Kaya Yoshida, Hiroshi Kamioka, Hirohiko Okamura

BioFactors 47 ( 6 ) 992 - 1015 2021.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

This study aimed to reveal the possible mechanisms by which O-linked-N-acetylglucosaminylation (O-GlcNAcylation) regulates osteoblast differentiation using a series of bioinformatics-oriented experiments. To examine the influence of O-GlcNAcylation levels on osteoblast differentiation, osteoblastic MC3T3-E1 cells were treated with O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) inhibitors. Correlations between the levels of O-GlcNAcylation and the expression of osteogenic markers as well as OGT were evaluated by qPCR and western blotting. The O-GlcNAcylated proteins assumed to correlate with Runx2 expression were retrieved from several public databases and used for further bioinformatics analysis. Following the findings of the bioinformatics analysis, intracellular calcium ([Ca2+ ]i ) was monitored in the cells treated with OGT and OGA inhibitors using a confocal laser-scanning microscope (CLS). The interaction effect between O-GlcNAcylation and [Ca2+ ]i on osteogenic marker expression was determined using stable OGT knockdown MC3T3-E1 cells. O-GlcNAcylation was positively associated with osteoblast differentiation. The time-course profile of global O-GlcNAcylated proteins showed a distinctive pattern with different molecular weights during osteoblast differentiation. The expression pattern of several O-GlcNAcylated proteins was significantly similar to that of Runx2 expression. Bioinformatic analysis of the retrieved Runx2-related-O-GlcNAcylated-proteins revealed the importance of [Ca2+ ]i . CLS showed that alteration of O-GlcNAcylation rapidly changed [Ca2+ ]i in MC3T3-E1 cells. O-GlcNAcylation and [Ca2+ ]i showed an interaction effect on the expression of osteogenic markers. OGT knockdown disrupted the [Ca2+ ]i -induced expression changes of osteogenic markers. O-GlcNAcylation interacts with [Ca2+ ]i and elicits osteoblast differentiation by regulating the expression of osteogenic markers.

DOI： 10.1002/biof.1774

PubMed

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/biof.1774

RFX1‐mediated CCN3 induction that may support chondrocyte survival under starved conditions Reviewed International journal

Tomomi Mizukawa, Takashi Nishida, Sho Akashi, Kazumi Kawata, Sumire Kikuchi, Harumi Kawaki, Masaharu Takigawa, Hiroshi Kamioka, Satoshi Kubota

Journal of Cellular Physiology 236 ( 10 ) 6884 - 6896 2021.10

Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Cellular communication network factor (CCN) family members are multifunctional matricellular proteins that manipulate and integrate extracellular signals. In our previous studies investigating the role of CCN family members in cellular metabolism, we found three members that might be under the regulation of energy metabolism. In this study, we confirmed that CCN2 and CCN3 are the only members that are tightly regulated by glycolysis in human chondrocytic cells. Interestingly, CCN3 was induced under a variety of impaired glycolytic conditions. This CCN3 induction was also observed in two breast cancer cell lines with a distinct phenotype, suggesting a basic role of CCN3 in cellular metabolism. Reporter gene assays indicated a transcriptional regulation mediated by an enhancer in the proximal promoter region. As a result of analyses in silico, we specified regulatory factor binding to the X-box 1 (RFX1) as a candidate that mediated the transcriptional activation by impaired glycolysis. Indeed, the inhibition of glycolysis induced the expression of RFX1, and RFX1 silencing nullified the CCN3 induction by impaired glycolysis. Subsequent experiments with an anti-CCN3 antibody indicated that CCN3 supported the survival of chondrocytes under impaired glycolysis. Consistent with these findings in vitro, abundant CCN3 production by chondrocytes in the deep zones of developing epiphysial cartilage, which are located far away from the synovial fluid, was confirmed in vivo. Our present study uncovered that RFX1 is the mediator that enables CCN3 induction upon cellular starvation, which may eventually assist chondrocytes in retaining their viability, even when there is an energy supply shortage.

DOI： 10.1002/jcp.30348

PubMed

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jcp.30348

Quantitative Evaluation of Osteocyte Morphology and Bone Anisotropic Extracellular Matrix in Rat Femur Reviewed

Takuya Ishimoto, Keita Kawahara, Aira Matsugaki, Hiroshi Kamioka, Takayoshi Nakano

Calcified Tissue International 109 ( 4 ) 434 - 444 2021.10

Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

<title>Abstract</title>Osteocytes are believed to play a crucial role in mechanosensation and mechanotransduction which are important for maintenance of mechanical integrity of bone. Recent investigations have revealed that the preferential orientation of bone extracellular matrix (ECM) mainly composed of collagen fibers and apatite crystallites is one of the important determinants of bone mechanical integrity. However, the relationship between osteocytes and ECM orientation remains unclear. In this study, the association between ECM orientation and anisotropy in the osteocyte lacuno-canalicular system, which is thought to be optimized along with the mechanical stimuli, was investigated using male rat femur. The degree of ECM orientation along the femur longitudinal axis was significantly and positively correlated with the anisotropic features of the osteocyte lacunae and canaliculi. At the femur middiaphysis, there are the osteocytes with lacunae that highly aligned along the bone long axis (principal stress direction) and canaliculi that preferentially extended perpendicular to the bone long axis, and the highest degree of apatite <italic>c</italic>-axis orientation along the bone long axis was shown. Based on these data, we propose a model in which osteocytes can change their lacuno-canalicular architecture depending on the mechanical environment so that they can become more susceptible to mechanical stimuli via fluid flow in the canalicular channel.

DOI： 10.1007/s00223-021-00852-1

Scopus

PubMed

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Other Link： https://link.springer.com/article/10.1007/s00223-021-00852-1/fulltext.html

Authors’ Response to Letter from Professor Birkedal Reviewed

Takuya Ishimoto, Hiroshi Kamioka, Takayoshi Nakano

Calcified Tissue International 110 ( 1 ) 144 - 145 2021.8

Authors’ Response to Letter from Professor Birkedal Reviewed

Takuya Ishimoto, Hiroshi Kamioka, Takayoshi Nakano

Calcified Tissue International 2021.8

歯科矯正用アンカースクリューを用いて上下顎前歯の圧下により治療したガミースマイルを示す過蓋咬合症例 Reviewed

片岡伴記, 星島光博, 上岡寛

中・四国矯正歯科学会雑誌 33 ( 1 ) 29 - 38 2021.8

Index of Orthognathic Functional Treatment Need (IOFTN)とSeverity and Outcome Assessment (SOA)を用いた外科的矯正治療の適応基準の検討：ケースコントロール研究 Reviewed

中村政裕, 金光恵, 石田朋子, 中西泰之, 岡直毅, 藤澤厚郎, 川邉紀章, 上岡寛

中・四国矯正歯科学会雑誌 33 ( 1 ) 1 - 8 2021.8

著しく狭窄した上顎歯列弓に対して早期に外科的口蓋急速拡大法を行った片側性口唇裂・口蓋裂症例 Reviewed

中西泰之, 星島光博, 橋本真奈, 上岡寛

中・四国矯正歯科学会雑誌 33 ( 1 ) 87 - 87 2021.8

A skeletal Class III facial asymmetry case with a canted occlusal plane treated by LeFort I with unilateral horseshoe osteotomy Reviewed

Tomoyo Tanaka, Mitsuhiro Hoshijima, Norie Yoshioka, Hiroshi Kamioka

Orthodontic Waves 80 ( 3 ) 171 - 183 2021.7

下垂体機能低下を伴う中隔視神経異形成症患者の矯正歯科治療症例 Reviewed

河野加奈, 柳田剛志, 井澤俊, 上岡寛

岡山歯学会雑誌 40 ( 1 ) 1 - 10 2021.6

A case of growing patient with occlusal cant and facial asymmetry associated with ocular torticollis Reviewed

植田紘貴, 難波裕生, 山城隆, 上岡寛

日本顎変形症学会雑誌 31 ( 2 ) 134 - 134 2021.5

Endonuclease increases efficiency of osteoblast isolation from murine calvariae Reviewed International journal

Yosuke Asano, Yoshinori Matsumoto, Jose La Rose, Fang He, Takayuki Katsuyama, Wang Ziyi, Shigetomo Tsuji, Hiroshi Kamioka, Robert Rottapel, Jun Wada

Scientific Reports 11 ( 1 ) 8502 - 8502 2021.4

Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

<title>Abstract</title>Bone is a highly dynamic organ that undergoes remodeling equally regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. To clarify the regulation of osteoblastogenesis, primary murine osteoblasts are required for an in vitro study. Primary osteoblasts are isolated from neonatal calvariae through digestion with collagenase. However, the number of cells collected from one pup is not sufficient for further in vitro experiments, leading to an increase in the use of euthanized pups. We hypothesized that the viscosity of digested calvariae and digestion solution supplemented with collagenase results in cell clumping and reduction of isolated cells from bones. We simply added Benzonase, a genetically engineered endonuclease that shears all forms of DNAs/RNAs, in order to reduce nucleic acid-mediated viscosity. We found that addition of Benzonase increased the number of collected osteoblasts by three fold compared to that without Benzonase through reduction of viscosity. Additionally, Benzonase has no effect on cellular identity and function. The new osteoblast isolation protocol with Benzonase minimizes the number of neonatal pups required for an in vitro study and expands the concept that isolation of other populations of cells including osteocytes that are difficult to be purified could be modified by Benzonase.

DOI： 10.1038/s41598-021-87716-8

PubMed

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Other Link： http://www.nature.com/articles/s41598-021-87716-8

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Papers - KAMIOKA Hiroshi