Faculty Profiles - EKUNI Daisuke

MISC - EKUNI Daisuke

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Effects of periodontal therapy on circulating oxidative stress.

Tomofuji T, Ekuni D, Morita M

Springer 2014

Expression of reactive oxygen species in junctional and pocket epithelium.

ekuni D, Firth JD, Putnins EE

Springer 2014

Periodontitis and liver diseases.

Irie K, Ekuni D, Tomofuji T, Morita M

Springer 2014

Periodontitis and atherosclerosis.

Ekuni D, Tomofuji T, Morita M

Springer 2014

アルコール摂取後の口腔保健行動の変化と歯周病との関連

山根真由, 江國大輔, 水谷慎介, 町田達哉, 片岡広大, 丸山貴之, 森田学

岡山歯学会雑誌 32 ( 2 ) 87 - 88 2013.12

若年者における風邪の発症頻度と智歯の萠出状態との関連

町田達哉, 水谷慎介, 片岡広太, 山根真由, 丸山貴之, 友藤孝明, 江國大輔, 森田学

岡山歯学会雑誌 32 ( 2 ) 87 - 87 2013.12

咬合異常はラット海馬のベータアミロイドを誘導する

江國大輔

岡山歯学会雑誌 32 ( 2 ) 77 - 78 2013.12

Relationships between self-rated oral health, subjective symptoms, oral health behavior and clinical conditions in Japanese university students: a cross-sectional survey at Okayama University International journal

Azusa Kojima, Daisuke Ekuni, Shinsuke Mizutani, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

BMC ORAL HEALTH 13 ( 1 ) 62 - 62 2013.11

Language：English Publisher：BIOMED CENTRAL LTD

Background: Self-rated oral health is a valid and useful summary indicator of overall oral health status and quality of life. However, few studies on perception of oral health have been conducted among Japanese young adults. This study investigated whether oral health behavior, subjective oral symptoms, or clinical oral status were associated with self-rated oral health in Japanese young adults.
Methods: This cross-sectional survey included 2,087 students (1,183 males, 904 females), aged 18 and 19 years, at Okayama University, Japan. A self-administered questionnaire was distributed and an oral examination was performed.
Results: In a structural equation modeling analysis, the score of decayed, missing and filled teeth (DMFT) significantly affected self-rated oral health (p <0.05) and the effect size was highest. Malocclusion, subjective symptoms of temporomandibular disorders (TMD) and stomatitis, and poor oral health behavior significantly induced self-rated poor oral health with small effect sizes (p <0.05). Clinical periodontal conditions and Oral Hygiene Index-simplified were not related to self-rated oral health.
Conclusion: Self-rated oral health was influenced by subjective symptoms of TMD and stomatitis, oral health behavior, the score of DMFT, and malocclusion. The evaluation of these parameters may be a useful approach in routine dental examination to improve self-rated oral health in university students.

DOI： 10.1186/1472-6831-13-62

Effects of apoE Deficiency and Occlusal Disharmony on Amyloid-Beta Production and Spatial Memory in Rats International journal

Daisuke Ekuni, Yasumasa Endo, Takaaki Tomofuji, Tetsuji Azuma, Koichiro Irie, Kenta Kasuyama, Manabu Morita

PLoS ONE 8 ( 9 ) e74966 2013.9

Language：English

Amyloid-β (Aβ) plays a causative role in Alzheimer's disease. Apolipoprotein E (apoE) is involved in Aβ accumulation, whereas occlusal disharmony increases Aβ production in the rat hippocampus. The purpose of the present study was to investigate the effects of apoE deficiency and occlusal disharmony on Aβ production and spatial memory. Wild-type (WT) (n = 12) and apoE-deficient [ApoE(-/-)] (n = 12) rats (Sprague-Dawley
8 weeks old) were used. These rats were randomly divided into four groups of six rats each: two control (C) groups: WT (C-WT) and ApoE [C-ApoE(-/-)], and two occlusal disharmony (D) groups: WT (D-WT) and ApoE [D-ApoE(-/-)]. The C group received no treatment for 8 weeks. In the D group, the maxillary molar cusps were cut off for 8 weeks. The spatial memory of rats was assessed according to their behavioral performance in a radial arm maze. In both genotypes of rats, significant differences in the reference memory, Aβ42 production, β-secretase expression and plasma corticosterone levels were observed between the C and D groups (P &lt
0.0125). The levels of Aβ42 and glucocorticoid receptor in the C-ApoE(-/-) group were also significantly higher than those in the C-WT group (P &lt
0.0125). However, no significant differences in these parameters were found between the two genotypes with occlusal disharmony. In conclusion, occlusal disharmony induces cognitive dysfunction and Aβ accumulation in the rat hippocampus, and the effects of occlusal disharmony on Aβ accumulation and cognitive dysfunction were larger than those of apoE deficiency. © 2013 Ekuni et al.

DOI： 10.1371/journal.pone.0074966

Scopus

PubMed

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還元型コエンザイムQ10の歯周組織の加齢変化に対する効果について

米田俊樹, 友藤孝明, 江國大輔, 東哲司, 遠藤康正, 粕山健太, 町田達哉, 森田学

Journal of Oral Biosciences Supplement 2013 170 - 170 2013.9

Anti-aging Effects of Co-enzyme Q10 on Periodontal Tissues

T. Yoneda, T. Tomofuji, D. Ekuni, T. Azuma, Y. Endo, K. Kasuyama, T. Machida, M. Morita

JOURNAL OF DENTAL RESEARCH 92 ( 8 ) 735 - 739 2013.8

Language：English Publisher：SAGE PUBLICATIONS INC

Oxidative stress is associated with age-related reactions. The anti-oxidative effects of a reduced form of co-enzyme Q10 (rCoQ10) suppress oxidative stress, which may contribute to the prevention of age-related inflammatory reactions. We examined the effects of topically applied rCoQ10 on periodontal inflammatory reactions in a rat aging model. Male Fischer 344 rats, 2 (n = 6) and 4 mos (n = 18) of age, were used. All of the two-month-old rats and 6 of the four-month-old rats were sacrificed and 12 remaining four-month-old rats received topically applied ointment with or without 1% rCoQ10 on the gingival surface until they reached 6 mos of age. The rats showed an age-dependent increase in circulating oxidative stress. RCoQ10 decreased oxidative DNA damage and tartrate-resistant acid-phosphatase-positive osteoclasts in the periodontal tissue at 6 mos of age as compared with the control. The same conditions lowered gene expression of caspase-1 and interleukin-1 in the periodontal tissue. Furthermore, Nod-like receptor protein 3 inflammasomes were less activated in periodontal tissues from rCoQ10-treated rats as compared with the control rats. Our results suggest that rCoQ10 suppresses age-related inflammatory reactions and osteoclast differentiation by inhibiting oxidative stress.

DOI： 10.1177/0022034513490959

Effects of eating behaviors on being overweight in Japanese university students: A cross-sectional survey at the Okayama University International journal

Daisuke Ekuni, Michiko Furuta, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

Asia-Pacific Journal of Public Health 25 ( 4 ) 326 - 334 2013.7

Language：English

Being overweight is an important risk factor for lifestyle-related diseases. The objective of the present study was to examine whether eating until full, eating quickly, or both eating behaviors were associated with being overweight, defined as a body mass index (BMI) ≥ 25, in young adults. This cross-sectional survey comprised 1918 students (1139 male and 779 female students) at the Okayama University in Japan. In logistic regression analysis, eating until full was not associated with being overweight in either sex. The adjusted odds ratio of being overweight among those who reported eating quickly was 3.93 (2.45-6.31
P &lt
.0001) for male and 1.59 (0.79-3.21
P =.193) for female students. Moreover, the adjusted odds ratio of being overweight was 2.72 (1.72-4.30
P &lt
.001) in male students who frequently reported eating a fatty diet. The combination of eating quickly and frequently eating a fatty diet had a supra-additive effect on being overweight in the male students. © 2011 APJPH.

DOI： 10.1177/1010539511425702

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Relationship between masticatory performance and heart rate variability: A pilot study International journal

Noriko Takeuchi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

ACTA ODONTOLOGICA SCANDINAVICA 71 ( 3-4 ) 807 - 812 2013.5

Language：English Publisher：TAYLOR & FRANCIS LTD

Objective. Little information is available on how impaired masticatory performance relates to heart-rate variability (HRV). The aim of this study was to investigate the relationship between HRV indices and masticatory performance. Materials and methods. Sixty-five subjects (19 men and 46 women, 66.1 +/- 9.9 years old) who had received periodontal maintenance care at the Clinic of Preventive Dentistry of Okayama University Hospital were selected for the study. All subjects completed written questionnaires, a chewing (colour-changing gum) test for masticatory performance and measurement of occlusal force and HRV as well as oral examination. Results. The high sympathetic activity (LF >= 49.6) group showed a significantly lower level of masticatory performance (a(star) value) than the low sympathetic activity group (p < 0.05). There were also significant correlations of masticatory performance with LF and LF/HF (p < 0.05). Conclusion. These findings suggest that there is relationship between masticatory performance and HRV indices and impaired masticatory performance may be a risk factor for inducing high sympathetic activity.

DOI： 10.3109/00016357.2012.734403

Relationship between periodontal inflammation and fetal growth in pregnant women: a cross-sectional study International journal

Noriko Takeuchi, Daisuke Ekuni, Koichiro Irie, Michiko Furuta, Takaaki Tomofuji, Manabu Morita, Tatsuo Watanabe

ARCHIVES OF GYNECOLOGY AND OBSTETRICS 287 ( 5 ) 951 - 957 2013.5

Language：English Publisher：SPRINGER HEIDELBERG

Periodontal disease in pregnant women, evaluated by probing pocket depth (PPD) or clinical attachment level (CAL), significantly increases the risk of subsequent preterm birth (PB) and/or low birth weight (LBW). However, PPD and CAL do not always reflect current periodontal tissue inflammation. Therefore, assessment of bleeding on probing index (BOP), an indicator of periodontal inflammation, may be appropriate. The objective of this cross-sectional study was to investigate the relationship between periodontal inflammation and fetal growth in pregnant women after periodontal treatment.
We recruited 203 pregnant women (mean age 31.8 +/- A 4.5 years), including 20 subjects with periodontal inflammation and 183 periodontally healthy controls, after periodontal treatment. Fetal growth parameters as well as periodontal conditions were recorded.
Perinatal gestational age was 39.4 +/- A 1.3 weeks; 8 subjects had a PB and 12 had an infant with a LBW. Results of multiple stepwise regression analysis indicated that birth length was negatively correlated with BOP% (beta = -0.175; p = 0.002). Fetal femur length, and birth length and weight among mothers with low periodontal inflammation were significantly higher than those among mothers with high periodontal inflammation (p < 0.05). The LBW rate in the high periodontal inflammation group was significantly higher than that in the low periodontal inflammation group (p < 0.05).
These results suggest that periodontal inflammation is correlated with fetal femur length, birth weight, and birth length.

DOI： 10.1007/s00404-012-2660-4

P-22 岡山大学病院口臭外来における6年間の患者実態調査(ポスター)

貞森なみ子, 竹内倫子, 江國大輔, 森田学

口腔衛生学会雑誌 63 ( 2 ) 176 - 176 2013.4

P-36 アポリポ蛋白E欠損と咬合異常によるラット海馬および認知障害への影響(ポスター)

江國大輔, 遠藤康正, 友藤孝明, 東哲司, 入江浩一郎, 粕山健太, 米田俊樹, 森田学

口腔衛生学会雑誌 63 ( 2 ) 183 - 183 2013.4

P-56 歯周治療のメインテナンス期における歯周病の進行とコーヒー摂取頻度との関連(ポスター)

東哲司, 友藤孝明, 江國大輔, 入江浩一郎, 遠藤康正, 粕山健太, 水谷慎介, 町田達哉, 森田学

口腔衛生学会雑誌 63 ( 2 ) 193 - 193 2013.4

Effects of electrical stimulation on periodontal tissue remodeling in rats

T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, K. Kasuyama, M. Nagayama, M. Morita

JOURNAL OF PERIODONTAL RESEARCH 48 ( 2 ) 177 - 183 2013.4

Language：English Publisher：WILEY-BLACKWELL

Background and Objective Electric current is used to promote wound healing. However, it is unclear whether electrical stimulation contributes to gingival tissue remodeling. This study examined the effects of electrical stimulation on gingival tissue remodeling in a rat periodontitis model. Material and Methods Male Wistar rats (n=28, 8wks of age) were divided into four groups of seven rats each. The control group did not receive any treatment for 6wks. In the other groups, periodontitis was ligature-induced for 4wks. After 4wks, the rats with periodontitis were given daily electrical stimulation of 0, 50 or 100A for 2wks. Results The periodontitis group stimulated with 0 A showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue compared with the control group (p<0.05). The two remaining groups treated with 50or 100A of electrical stimulation exhibited a lower density of polymorphonuclear leukocytes (p<0.05) and a higher density of collagen than the group stimulated with 0A (p<0.05). They also showed higher expression of fibroblast growth factor-2 than the group treated with 0A of electrical stimulation (p<0.05). Conclusion Electric stimulation may offer a novel approach to promote gingival tissue remodeling in periodontal lesions.

DOI： 10.1111/j.1600-0765.2012.01518.x

大学生における頬粘膜圧痕の形成にかかわる因子

水谷慎介, 江國大輔, 友藤孝明, 入江浩一郎, 東哲司, 森田学

口腔衛生学会雑誌 63 ( 3 ) 291 - 291 2013.4

Lipopolysaccharide induces a stromal-epithelial signalling axis in a rat model of chronic periodontitis International journal

James D. Firth, Daisuke Ekuni, Koichiro Irie, Takaaki Tomofuji, Manabu Morita, Edward E. Putnins

JOURNAL OF CLINICAL PERIODONTOLOGY 40 ( 1 ) 8 - 17 2013.1

Language：English Publisher：WILEY-BLACKWELL

Aim Lipopolysaccharide is a bacterial virulence factor implicated in chronic periodontitis, which may penetrate the junctional epithelial barrier and basement membrane to insult underlying stroma. We sought to identify lipopolysaccharide-induced global gene expression changes responsible for signalling between stroma and epithelium during disease onset.
Materials and Methods Using a rat lipopolysaccharide periodontitis model, junctional epithelium and underlying stromal tissue were separately collected from healthy and diseased animals by laser-capture microdissection and subject to gene expression microarray analysis. Key gene products identified were validated in gingival epithelial and fibroblast cell cultures.
Results Global gene expression patterns distinguishing health versus disease were found in and between both tissue types. In stroma, the most significantly altered gene ontology function group (Z >= 4.00) was cytokines, containing most significantly (+/- 2-fold; p < 0.05) upregulated genes amphiregulin, IL1-beta and Fas ligand, all positive, diffusible modulators of the epithelial growth factor receptor pathway. In epithelium, the most significant changes were in downregulated FOS-related antigen-1 gene, somatostatin receptor-2 gene and mucin-4 gene, all negative modulators of the epithelial growth factor receptor pathway.
Conclusion These results establish a periodontitis model for studying gene product interactions and suggests that the onset of junctional epithelial disease hyperproliferation involves a concerted stromalepithelial signalling axis.

DOI： 10.1111/jcpe.12023

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