Faculty Profiles - EKUNI Daisuke

MISC - EKUNI Daisuke

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ラット歯周病モデルにおける血清miRNAと肝臓への影響

杉浦嘉雄, 米田俊樹, 藤森浩平, 丸山貴之, 宮井久敬, 小林暉政, 江國大輔, 友藤孝明, 森田学

岡山歯学会雑誌 37 ( 2 ) 81 - 81 2018.12

化学放射線療法中の口腔粘膜炎管理を行った症例

益成美保, 水野裕文, 丸山貴之, 横井彩, 小林暉政, 佐々木禎子, 志茂加代子, 三浦留美, 水川展吉, 江國大輔, 森田学

口腔衛生学会雑誌 68 ( 4 ) 231 - 237 2018.10

Language：Japanese Publisher：(一社)日本口腔衛生学会

頭頸部がんに対する化学放射線療法(CRT)の有害事象である口腔粘膜炎による疼痛は、患者のQOLを低下させ、がん治療の完遂を困難にする。今回、われわれは術後CRTを受けている患者に対して口腔管理を行い、口腔粘膜炎および疼痛を制御した結果、がん治療の完遂に貢献できた症例を報告する。患者は67歳、男性である。右側舌がん(T2N2bM0)に対して舌可動部半側切除術、右側頸部郭清術、遊離前外側大腿皮弁による再建術を施行した。術後の病理組織検査より切除断端陽性、頸部リンパ節の節外浸潤を認めたため、術後CRT(抗がん剤:シスプラチン、5-フルオロウラシル;2クール、強度変調放射線治療60Gy)を行うこととなった。CRT前から、口腔管理および衛生実地指導を継続して行った。CRT開始時はプラークの除去を中心とした口腔管理を行った。22Gy照射時点で口腔粘膜炎(Grade2)が発症したものの、その後軽度疼痛を認める程度で経過していた。42Gy照射時点で口腔粘膜炎の悪化(Grade3)および自発痛・接触痛を認めたため、治療完遂まで毎日、歯科衛生士がプラークの除去に加え、口腔粘膜保護を目的とした口腔管理を行った。その結果、56Gy照射時点で口腔粘膜炎は改善傾向を示し(Grade2)、疼痛も緩和され、CRT完遂に至った。術後CRT開始直後から口腔粘膜炎の状態に応じた口腔管理を行うことで、疼痛の制御が成功し、CRTの完遂に貢献できたと考えられる。(著者抄録)

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Relationship between severity of periodontitis and masseter muscle activity during waking and sleeping hours

Seiya Kato, Daisuke Ekuni, Shigehisa Kawakami, Acing Habibie Mude, Manabu Morita, Shogo Minagi

Archives of Oral Biology 90 13 - 18 2018.6

Language：English Publisher：Elsevier Ltd

Objective: This study was conducted to investigate whether the masseter muscle activity shows any specific pattern in relation to the severity of periodontitis. Design: Sixteen subjects with no or mild periodontitis (NMP group) and 15 subjects with moderate or severe periodontitis (MSP group) were enrolled. Plasma IgG antibody titer was examined using Porphyromonas gingivalis as a bacterial antigen. Surface electromyography (EMG) of the masseter muscles was continuously recorded using an ambulatory surface EMG recording device while patients were awake and asleep. Masseter muscle activity was analyzed using intensities of 5%–10% maximal voluntary clenching (MVC), 10%–20% MVC, and &gt
20% MVC. Furthermore, EMG levels of 20% MVC were adopted as the threshold for analysis of phasic, tonic, and mixed EMG activities. The cumulative duration of masseter muscle activity and bruxism episodes was calculated as duration per hour. Results: There was no significant difference in plasma IgG antibody titers against P. gingivalis between the NMP and MSP groups (p = 0.423). During waking hours, the duration of masseter muscle activity with an intensity of &gt
20% MVC was significantly longer in the MSP group than in the NMP group (p = 0.037). During sleeping hours, the duration of masseter muscle activity at all MVC intensities was significantly longer in the MSP group than in the NMP group (all p &lt
0.05). Additionally, the duration of phasic and mixed episodes was significantly longer in the MSP group than those in the NMP group while both awake and asleep (all p &lt
0.05). Conclusions: The results of this study suggested that masseter muscle activity might be related to the severity of periodontitis.

DOI： 10.1016/j.archoralbio.2018.02.021

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口腔内ジメチルサルファイド濃度と全身疾患および服薬との関係

横井彩, 竹内倫子, 宮井久敬, 江國大輔, 森田学

日本口臭学会会誌 9 ( 1 ) 38 - 39 2018.5

歯周組織における加齢に伴う酸化ストレスの増加に対するコーヒー摂取の影響

小林暉政, 東哲司, 杉浦嘉雄, 宮井久敬, 米田俊樹, 丸山貴之, 江國大輔, 友藤孝明, 森田学

口腔衛生学会雑誌 68 ( 増刊 ) 182 - 182 2018.4

大学生における口腔関連QOLの変化に影響を及ぼす因子の検討縦断研究

佐保輝, 竹内真由, 稲生智秋, Monirul Islam Md, 杉浦嘉雄, 外山直樹, 内田瑶子, 横井彩, 谷口綾乃, 片岡広太, 米田俊樹, 東哲司, 江國大輔, 森田学

口腔衛生学会雑誌 68 ( 増刊 ) 109 - 109 2018.4

歯科衛生士教育における模擬患者の違いによる実習効果の比較

木村優子, 江國大輔, 森田学, 太田正美

口腔衛生学会雑誌 68 ( 増刊 ) 129 - 129 2018.4

Unusual oral mucosal microbiota after hematopoietic cell transplantation with glycopeptide antibiotics: potential association with pathophysiology of oral mucositis International journal

Misato Muro, Yoshihiko Soga, Tomoko Higuchi, Kota Kataoka, Daisuke Ekuni, Yoshinobu Maeda, Manabu Morita

Folia Microbiologica 63 ( 5 ) 1 - 11 2018.3

Language：English Publisher：Springer Netherlands

Severe oral mucositis occurs frequently in patients receiving hematopoietic stem cell transplantation (HCT). Oral mucosal bacteria can be associated with progression of oral mucositis, and systemic infection may occur via ulcerative oral mucositis. However, little information is available regarding the oral microbiota after HCT. Here, PCR-denaturing gradient gel electrophoresis (DGGE) was performed to characterize the oral mucosal microbiota, which can be affected by antibiotics, before and after HCT. Sixty reduced-intensity HCT patients were enrolled. Three patients with the least antibiotic use (quinolone prophylaxis and/or β-lactam monotherapy group) and three patients with the most antibiotic use (β-lactam-glycopeptide combination therapy group) were selected. Bacterial DNA samples obtained from the oral mucosa before and after HCT were subjected to PCR-DGGE. The trajectory of oral mucositis was evaluated. The oral mucosal microbiota in the β-lactam-glycopeptide combination therapy group was different from that in the quinolone prophylaxis and/or β-lactam monotherapy group, and Staphylococcus spp. and Enterococcus spp. were identified. Lautropia mirabilis was dominant in one patient. Ulcerative oral mucositis was observed only in the β-lactam-glycopeptide combination therapy group. In conclusion, especially with the use of strong antibiotics, such as glycopeptides, the oral mucosal microbiota differed completely from that under normal conditions and consisted of Staphylococcus spp., Enterococcus spp., and unexpectedly L. mirabilis. The normal oral microbiota consists not only of bacteria, but these unexpected bacteria could be involved in the pathophysiology as well as systemic infection via oral mucositis. Our results can be used as the basis for future studies in larger patient populations.

DOI： 10.1007/s12223-018-0596-1

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Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium

Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Daiki Fukuhara, Yoko Uchida, Kota Kataoka, Shuichiro Kobayashi, Takeshi Kikuchi, Akio Mitani, Yoshihiro Shimazaki, Manabu Morita

Archives of Oral Biology 87 72 - 78 2018.3

Language：English Publisher：Elsevier Ltd

Objective Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4+ T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. Design GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age-related changes in alveolar bone were quantified using micro-CT analysis. Results SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P &lt
0.01). SPF mice at 22 weeks of age increased expression of CD4+ T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P &lt
0.01). Furthermore, there was increased CD4+ T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4+ T cells, CCL5, IL-17A and RANKL expression between the two age groups. Conclusions SPF mice induced an age-related increase in CD4+ T cells co- expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4+ T cell migration and cytokine expression.

DOI： 10.1016/j.archoralbio.2017.12.007

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職域における早食い、口腔内状態およびメタボリックシンドローム発症との関連

横井彩, 江國大輔, 米田俊樹, 森田学

口腔衛生学会雑誌 68 ( 1 ) 9 - 14 2018.1

Language：Japanese Publisher：(一社)日本口腔衛生学会

メタボリックシンドローム(Metabolic Syndrome:MetS)のリスク因子として早食いが挙げられる。また近年、MetsSは歯の喪失やう蝕など口腔内状態との関連も注目されている。しかし、MetS、早食いおよび口腔内状態を総合的に検討した報告は少ない。そこで、本縦断研究は、職域における早食いおよび口腔内状態とMetS発症との関連について検討することを目的とした。対象者は、某事業所における平成24・27年度定期健診をどちらも受診した従業員のうち、平成24年度MetSに該当しなかった男性114名(36〜63歳)を分析対象者とした。診査項目は、身長、体重、腹囲、血圧、空腹時血糖値、中性脂肪、HDLコレステロール値、口腔内状態、生活習慣(早食いの有無、食・運動・飲酒・喫煙習慣)とした。平成27年度健診結果から、MetS発症群と非発症群に分け、早食いの習慣および口腔内状態との関連を分析した。統計分析にはχ2検定、Fisherの直接法およびMann-Whitney U検定を用いた。3年後にMetSを発症した者(16名)は、全員早食いの自覚があった。また、MetS発症において、早食いと腹囲異常による相加効果がみられた。結論として、早食いで腹囲異常のある者はMetS発症のリスクが高いことがわかった。一方で、口腔内状態との直接的な関連はみられなかった。(著者抄録)

DOI： 10.5834/jdh.68.1_9

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Type D personality and periodontal disease in university students: A prospective cohort study. International journal

Shinsuke Mizutani, Daisuke Ekuni, Mayu Yamane-Takeuchi, Tetsuji Azuma, Ayano Taniguchi-Tabata, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

Journal of health psychology 23 ( 5 ) 754 - 762 2018

Language：English

The purpose of this prospective cohort study was to investigate whether Type D personality was related to periodontal disease in Japanese university students. Among students ( n = 600) who were interested in receiving oral health examinations, logistic regression analysis showed that the risk of developing periodontal disease during a 3-year period was associated with body mass index ⩾ 25 (odds ratio: 2.543; 95% confidence interval: 1.297-4.989; p = 0.007) and Type D personality (odds ratio: 1.473; 95% confidence interval: 1.027-2.111; p = 0.035). In this short-term prospective cohort study, a significant association between periodontal disease and Type D personality was observed in Japanese university students.

DOI： 10.1177/1359105316668668

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The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trial International journal

Hirofumi Mizuno, Daisuke Ekuni, Takayuki Maruyama, Kota Kataoka, Toshiki Yoneda, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Jun Wada, Manabu Morita

PLOS ONE 12 ( 11 ) 25 - 36 2017.11

Language：English Publisher：PUBLIC LIBRARY SCIENCE

Aim
The purpose of this study was to investigate the effects of non-surgical periodontal treatment on hemoglobinA1c (HbA1c) levels, oxidative stress balance and quality of life (QOL) in patients with type 2 diabetes mellitus (T2DM) compared to no periodontal treatment (simple oral hygiene instructions only).
Methods
The design was a 6-month, single-masked, single center, randomized clinical trial. Patients had both T2DM and chronic periodontitis. Forty participants were enrolled between April 2014 and March 2016 at the Nephrology, Diabetology and Endocrinology Department of Okayama University Hospital. The periodontal treatment group (n = 20) received non-surgical periodontal therapy, including scaling and root planing plus oral hygiene instructions, and consecutive supportive periodontal therapy at 3 and 6 months. The control group (n = 17) received only oral hygiene instructions without treatment during the experimental period. The primary study outcome was the change in HbA1c levels from baseline to 3 months. Secondary outcomes included changes in oxidative stress balance (Oxidative-INDEX), the Diabetes Therapy-Related QOL and clinical periodontal parameters from baseline to 3 months and baseline to 6 months.
Results
Changes in HbA1c in the periodontal treatment group were not significantly different with those in the control group at 3 and 6 months. Systemic oxidative stress balance and QOL significantly improved in the periodontal treatment group compared to the control group at 3 months. In the subgroup analysis (moderately poor control of diabetes), the decrease in HbA1c levels in the periodontal treatment group was greater than that in the control group at 3 months but not significant.
Conclusions
In T2DM patients, non-surgical periodontal treatment improved systemic oxidative stress balance and QOL, but did not decrease HbA1c levels at 3 months follow-up.

DOI： 10.1371/journal.pone.0188171

大学生における歯周炎と学部系統との関連

谷口綾乃, 江國大輔, 竹内真由, 森田学

日本公衆衛生学会総会抄録集 76回 464 - 464 2017.10

Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats International journal

Hisataka Miyai, Takayuki Maruyama, Takaaki Tomofuji, Toshiki Yoneda, Tetsuji Azuma, Hirofumi Mizuno, Yoshio Sugiura, Terumasa Kobayashi, Daisuke Ekuni, Manabu Morita

ARCHIVES OF ORAL BIOLOGY 82 247 - 255 2017.10

Language：English Publisher：PERGAMON-ELSEVIER SCIENCE LTD

Objective: The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats.
Design: Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus.
Results: After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P < 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P < 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P < 0.05).
Conclusions: Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

DOI： 10.1016/j.archoralbio.2017.06.025

大学生における歯ぎしりの自覚と不正咬合との関連前向きコホート研究

外山直樹, 江國大輔, 入江浩一郎, 竹内真由, 谷口綾乃, 森田学

日本公衆衛生学会総会抄録集 76回 317 - 317 2017.10

Hyperlipidemia is involved in apoptosis in rat submandibular glands International journal

Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Toshiki Yoneda, Yoshihiro Shimazaki, Manabu Morita

ARCHIVES OF ORAL BIOLOGY 81 136 - 140 2017.9

Language：English Publisher：PERGAMON-ELSEVIER SCIENCE LTD

Objective: The aim of the present study was to investigate the effects of hyperlipidemia on histological changes and apoptosis in submandibular glands using apolipoprotein E (apoE)-deficient rats.
Design: Histopathological findings related to induced apoptosis in the submandibular glands were compared between apoE-deficient rats (n = 6; male; age, 16 weeks) and the corresponding wild-type rats (n = 6).
Results: ApoE-deficient rats showed significantly higher plasma levels of oxidized low-density lipoprotein (LDL), total cholesterol, very LDL and LDL, and lower plasma levels of high-density lipoprotein when compared to control rats (P < 0.05). Lipid deposition in the submandibular gland was observed in apoE-deficient rat group and in none of the control group. Significant increases in vacuolization and apoptosis in acinar cells were observed in apoE-deficient rats, as compared to control rats (P < 0.05). The number of active caspase-3-positive cells was also higher in the apoE-deficient rat group when compared with the control group (P < 0.01).
Conclusions: According to our results, hyperlipidemia induced apoptosis in apoE-deficient rat submandibular glands. Oxidized LDL generation in case of hyperlipidemia may trigger off a reaction of apoptotic acinar cells with vacuolization in the submandibular glands.

DOI： 10.1016/j.archoralbio.2017.05.004

大学病院に勤務する医療技術部門職員および事務部職員における歯科用語の認知度の違い

城本ゆき子, 三浦留美, 高坂由紀奈, 江國大輔, 佐々木朗, 飯田征二

日本歯科衛生学会雑誌 12 ( 1 ) 157 - 157 2017.8

勤務形態が口腔保健行動および口腔状態に与える影響

外山直樹, 入江浩一郎, 谷口綾乃, 江國大輔, 友藤孝明, 森田学

口腔衛生学会雑誌 67 ( 3 ) 206 - 206 2017.7

抗がん剤投与ラットへのカテキン局所塗布による抗酸化作用の検討

小林暉政, 宮井久敬, 丸山貴之, 友藤孝明, 東哲司, 米田俊樹, 杉浦嘉雄, 江國大輔, 森田学

口腔衛生学会雑誌 67 ( 3 ) 203 - 203 2017.7

Associations between dental knowledge, source of dental knowledge and oral health behavior in Japanese university students: A cross-sectional study International journal

Ayano Taniguchi-Tabata, Daisuke Ekuni, Shinsuke Mizutani, Mayu Yamane-Takeuchi, Kota Kataoka, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

PLOS ONE 12 ( 6 ) 1 - 12 2017.6

Language：English Publisher：PUBLIC LIBRARY SCIENCE

The aim of this study was to investigate the associations between dental knowledge, the source of dental knowledge and oral health behavior in a group of students at a university in Japan. A total of 2,220 university students (1,276 males, 944 females) volunteered to undergo an oral examination and answer a questionnaire. The questionnaire assessed dental knowledge, the source of dental knowledge and oral health behavior (e.g., daily frequency of tooth brushing, use of dental floss and regular dental checkups). The odds ratio and 95% confidence interval for oral health behavior based on dental knowledge and source of dental knowledge were calculated using logistic regression models. Of the participants, 1,266 (57.0%) students obtained dental knowledge from dental clinics, followed by school (39.2%) and television (29.1%). Logistic regression analyses indicated that use of dental floss was significantly associated with source of dental knowledge from dental clinics (P = 0.006). Receiving regular dental checkups was significantly associated with source of dental knowledge; the positive source was dental clinic (P < 0.001) and the negative sources were school (P = 0.004) and television (P = 0.018). Dental clinic was the most common source of dental knowledge and associated with better oral health behavior among the Japanese university students in this study.

DOI： 10.1371/journal.pone.0179298

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