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Expression of Salivary miR-203a-3p Was Related with Oral Health-Related Quality of Life in Healthy Volunteers International journal

Terumasa Kobayashi, Takaaki Tomofuji, Tatsuya Machida, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Akiko Hirose, Manabu Morita

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18 ( 6 ) 372 - 373 2017.6

Language：English Publisher：MDPI AG

Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual's oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids could reflect various health conditions. The aim of this pilot study was to investigate whether salivary miRNAs expression differs according to OHRQoL in healthy volunteers. Forty-six volunteers (median age, 23.0 years) were recruited, and their OHRQoL was assessed using the Japanese version of the Oral Health Impact Profile (OHIP-J). Then, we compared salivary microRNA profiles of the high-OHRQoL group (<= 25th percentile score of OHIP-J) and the low-OHRQoL group (>= 75th percentile score of OHIP-J) using the polymerase chain reaction (PCR) array and the quantitative real-time PCR. There were no significant differences between the two groups in terms of oral health status. In the PCR array, miR-203a-3p and miR-30b-5p were significantly more expressed in the low-OHRQoL group (p < 0.05). Quantitative real-time PCR assay also showed that miR-203a-3p was more highly expressed in the low-OHRQoL group than in the high-OHRQoL group (p < 0.05). These observations suggest that expression of salivary miR-203a-3p was related with OHRQoL in healthy volunteers.

DOI： 10.3390/ijms18061263

Association between periodontitis and prognosis of pancreatobiliary tract cancer: A pilot study. International journal

Takayuki Maruyama, Takaaki Tomofuji, Tatsuya Machida, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Toshiki Yoneda, Hisataka Miyai, Hirofumi Mizuno, Daisuke Ekuni, Hiroyuki Okada, Manabu Morita

Molecular and clinical oncology 6 ( 5 ) 683 - 687 2017.5

Language：English

Several studies have indicated that periodontitis is a risk factor for cancer. However, the association between periodontitis and the prognosis of pancreatobiliary tract cancer remains unclear. The aim of this pilot study was to investigate the association between periodontitis and prognosis of pancreatobiliary tract cancer. A total of 22 patients diagnosed with pancreatobiliary tract cancer were analyzed. Oral health status, including severity of periodontitis, general health status and biochemical serum markers were evaluated. The Kaplan-Meier method and Cox proportional hazards model were used to assess factors affecting the prognosis of pancreatobiliary tract cancer. The Kaplan-Meier analysis demonstrated that low body mass index, high concentration of serum C-reactive protein (CRP) and severe periodontitis were significant prognostic factors for survival rate. The Cox proportional hazards model revealed that serum carbohydrate antigen 19-9 concentration [hazard ratio (HR)=1.002; 95% confidence interval (CI): 1.000-1.004] and serum CRP concentration (HR=2.57; 95% CI: 1.15-5.74) were significantly associated with the prognosis of pancreatobiliary tract cancer. In addition, cancer patients with severe periodontitis had higher serum CRP concentrations compared with those without severe periodontitis. Therefore, severe periodontitis indirectly affected the prognosis of pancreatobiliary tract cancer through promoting systemic inflammation.

DOI： 10.3892/mco.2017.1220

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唾液中microRNAと歯周病の重症度との関連

藤森浩平, 米田俊樹, 友藤孝明, 江國大輔, 東哲司, 丸山貴之, 町田達哉, 水野裕文, 森田学

口腔衛生学会雑誌 67 ( 増刊 ) 168 - 168 2017.4

2型糖尿病患者における非外科的歯周治療による酸化ストレスの改善ランダム化比較試験

杉浦嘉雄, 水野裕文, 福原大樹, 丸山貴之, 江國大輔, 米田俊樹, 片岡広太, 川端勇也, 水谷慎介, 友藤孝明, 森田学

口腔衛生学会雑誌 67 ( 増刊 ) 119 - 119 2017.4

口腔衛生学分野のテーマをアウトカム基盤型教育で行うには予防歯科のアウトカム基盤型教育

江國大輔

口腔衛生学会雑誌 67 ( 増刊 ) 77 - 78 2017.4

Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet International journal

Toshiki Yoneda, Takaaki Tomofuji, Muneyoshi Kunitomo, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Tatsuya Machida, Hisataka Miyai, Kouhei Fujimori, Manabu Morita

NUTRIENTS 9 ( 1 ) 830 - 833 2017.1

Language：English Publisher：MDPI AG

Obesity induces gingival oxidative stress, which is involved in the progression of alveolar bone resorption. The antioxidant effect of hydrogen-rich water may attenuate gingival oxidative stress and prevent alveolar bone resorption in cases of obesity. We examined whether hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption in obese rats fed a high-fat diet. Male Fischer 344 rats (n = 18) were divided into three groups of six rats each: a control group (fed a regular diet and drinking distilled water) and two experimental groups (fed a high-fat diet and drinking distilled water or hydrogen-rich water). The level of 8-hydroxydeoxyguanosine was determined to evaluate oxidative stress. The bone mineral density of the alveolar bone was analyzed by micro-computerized tomography. Obese rats, induced by a high-fat diet, showed a higher gingival level of 8-hydroxydeoxyguanosine and a lower level of alveolar bone density compared to the control group. Drinking hydrogen-rich water suppressed body weight gain, lowered gingival level of 8-hydroxydeoxyguanosine, and reduced alveolar bone resorption in rats on a high-fat diet. The results indicate that hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption by limiting obesity.

DOI： 10.3390/nu9010064

ラット歯周炎モデルに対する天然植物由来成分配合歯磨剤の効果.

米田俊樹, 東哲司, 友藤孝明, 江國大輔, 片岡広太, 山根真由, 川端勇也, 國友宗義, 森田学

口腔衛生学会雑誌 67 ( 1 ) 11 - 17 2017

Validity of a New Kit Measuring Salivary Lactate Dehydrogenase Level for Screening Gingivitis International journal

Daisuke Ekuni, Mayu Yamane-Takeuchi, Kota Kataoka, Aya Yokoi, Ayano Taniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoko Uchida, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Manabu Morita

DISEASE MARKERS 2017 2434 - 2441 2017

Language：English Publisher：HINDAWI LTD

Aim. The aim of this study was to determine the usefulness of a new kit that can evaluate salivary lactate dehydrogenase (LD) level in real time for screening gingivitis. Materials and Methods. The study included 70 systemic healthy volunteers [29 males and 41 females; mean age +/- SD: 24.1 +/- 2.6 years]. Resting saliva was collected from each participant and LD level was evaluated in real time using the kit (a color-changing sheet with an integer scale ranging from 1 to 10). A dentist measured probing pocket depth, clinical attachment level, and the proportion of sites with bleeding on probing (% BOP) at six sites on all teeth. Gingivitis was diagnosed when the BOP value was >= 20%. Results. Salivary LD level was positively correlated with mean % BOP (odds ratio: 1.47, 95% confidence interval: 1.132-1.916, and P < 0.001) in a logistic regression model. The sensitivity and specificity of the kit were 0.89 and 0.98, respectively, at a cut-off value of 8.0 for LD level. Conclusions. The new kit for measurement of salivary LD level may be a useful tool to screen for gingivitis in young adults, which contributes to early detection of future periodontitis.

DOI： 10.1155/2017/9547956

動機づけ面接法(MI)により高齢者の口腔衛生の改善ができた1症例.

千神八重子, 江國大輔, 森田学

口腔衛生学会雑誌 67 ( 1 ) 29 - 34 2017

Associations among oral health-related quality of life, subjective symptoms, clinical status, and self-rated oral health in Japanese university students: a cross-sectional study International journal

Mayu Yamane-Takeuchi, Daisuke Ekuni, Shinsuke Mizutani, Kota Kataoka, Ayano Taniguchi-Tabata, Tetsuji Azuma, Michiko Furuta, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

BMC ORAL HEALTH 16 ( 1 ) 127 - 127 2016.11

Language：English Publisher：BIOMED CENTRAL LTD

Background: The present study aimed to elucidate the associations among self-rated oral health, clinical oral health status, oral health behaviors, subjective oral symptoms, and oral health-related quality of life (OHRQoL) in a group of Japanese university students.
Methods: Of 2051 participants, 2027 (98.83%) students received an optional oral examination and answered a questionnaire including items regarding age, sex, self-rated oral health, oral health behaviors, subjective oral symptoms, and OHRQoL [The Oral Health Impact Profile (OHIP)-14]. On oral examination, the decayed, missing, and filled teeth (DMFT) score, Community Periodontal Index (CPI), the percentage of teeth showing bleeding on probing (%BOP), and malocclusion were recorded. Structural equation modelling (SEM) analysis was used to test associations.
Results: The mean score (+/- SD) of OHIP-14 was 1.92 +/- 5.47. In the SEM analysis, the final model showed that self-rated oral health, oral pain, malocclusion, and the DMFT score were directly associated with the OHRQoL, and subjective symptoms of temporomandibular disorders (TMD) and recurrent aphthous stomatitis were both directly and indirectly associated (p < 0.05). CPI, %BOP, and oral health behaviors were excluded from the final model.
Conclusions: OHRQoL was associated with self-related oral health, subjective symptoms of TMD, oral pain and stomatitis, DMFT, and malocclusion in this group of Japanese university students.

DOI： 10.1186/s12903-016-0322-9

Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice International journal

Kota Kataoka, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Muneyoshi Kunitomo, Yoko Uchida, Daiki Fukuhara, Manabu Morita

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17 ( 11 ) 2016.11

Language：English Publisher：MDPI AG

The aim of this study was to investigate whether a Keap1-dependent oxidative stress detector-luciferase (OKD-LUC) mouse model would be useful for the visualization of oxidative stress induced by experimental periodontitis. A ligature was placed around the mandibular first molars for seven days to induce periodontitis. Luciferase activity was measured with an intraperitoneal injection of D-luciferin on days 0, 1, and 7. The luciferase activity in the periodontitis group was significantly greater than that in the control group at seven days. The expressions of heme oxygenase-1 (HO-1) and malondialdehyde in periodontal tissue were significantly higher in the periodontitis group than in the control group. Immunofluorescent analysis confirmed that the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) occurred more frequently in the periodontitis group than in the control group. This study found that under oxidative stress induced by experimental periodontitis, the Nrf2/antioxidant defense pathway was activated and could be visualized from the luciferase activity in the OKD-LUC model. Thus, the OKD-LUC mouse model may be useful for exploring the mechanism underlying the relationship between the Nrf2/antioxidant defense pathway and periodontitis by enabling the visualization of oxidative stress over time.

DOI： 10.3390/ijms17111907

miR-1246 and miR-4644 in salivary exosome as potential biomarkers for pancreatobiliary tract cancer International journal

Tatsuya Machida, Takaaki Tomofuji, Takayuki Maruyama, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Hisataka Miyai, Hirofumi Mizuno, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Hiroyuki Okada, Manabu Morita

ONCOLOGY REPORTS 36 ( 4 ) 2375 - 2381 2016.10

Language：English Publisher：SPANDIDOS PUBL LTD

Pancreatobiliary tract cancer is a highly fatal cancer. Detection of pancreatobiliary tract cancer is difficult because it lacks typical clinical symptoms and because of its anatomical location. Biomarker discovery is therefore important to detect pancreatobiliary tract cancer in its early stage. A study demonstrated that expression levels of miR-1246, miR-3976, miR-4306, and miR-4644 in serum exosomes were higher in pancreatic cancer patients than these levels in healthy control participants. Supposing that microRNA (miRNA) expression profiles in saliva are similar to those in serum, four miRNAs (miR-1246, miR-3976, miR-4306, and miR-4644) in salivary exosomes may also be useful for detection of pancreatobiliary tract cancer. In this study, it was examined whether these miRNAs could be used as biomarkers for pancreatobiliary tract cancer. Twelve pancreatobiliary tract cancer patients and 13 healthy control participants were analyzed as a cancer and a control group, respectively. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. Using quantitative real-time PCR (RT-qPCR), the relative expression ratios of miR-1246 and miR-4644 were significantly higher in the cancer group than these ratios in the control group. Receiver operating characteristic (ROC) curves were constructed to analyze the discrimination power of these miRNAs. For miR-1246, the results yielded an area under the curve (AUC) of 0.814 (P=0.008). For miR-4644, the results yielded an AUC of 0.763 (P=0.026). For the combination of miR-1246 and miR-4644, the results yielded an increased AUC of 0.833 (P=0.005). This pilot study suggests that miR-1246 and miR-4644 in salivary exosomes could be candidate biomarkers for pancreatobiliary tract cancer.

DOI： 10.3892/or.2016.5021

MicroRNAs as serum biomarkers for periodontitis International journal

Takaaki Tomofuji, Toshiki Yoneda, Tatsuya Machida, Daisuke Ekuni, Tetsuji Azuma, Kota Kataoka, Takayuki Maruyama, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY 43 ( 5 ) 418 - 425 2016.5

Language：English Publisher：WILEY-BLACKWELL

Aim: Studies demonstrated that periodontitis modulates microRNA (miRNAs) expression rates in periodontal tissue. However, the relationship between periodontitis and miRNAs profile in circulation remains unclear. In this study, we investigated the effects of periodontitis on serum miRNAs profile in a rat model.
Material and Methods: Male Wistar rats (n=32, 8weeks old) were divided into four groups of eight rats each. The control groups received no treatment for 2 or 4weeks. In the other two groups, periodontitis was ligature induced for 2 or 4weeks. Serum miRNAs expression profiles of each group were compared.
Results: Ligation around teeth induced periodontal inflammation at 2weeks and periodontal tissue destruction at 4weeks. Microarray results showed that 25 miRNAs were expressed with a <0.5 or >2 difference between the control and periodontitis groups at 4weeks. Results of real-time PCR revealed that the periodontitis group up-regulated expression rates of serum miR-207 and miR-495 at 2weeks, and miR-376b-3p at 4weeks (p<0.05).
Conclusion: Serum miRNAs (miR-207, miR-495, and miR-376b-3p) could be valuable biomarkers for periodontitis.

DOI： 10.1111/jcpe.12536

食道癌の術後患者に対する歯科専門職による口腔のケアの効果

水野裕文, 水谷慎介, 丸山貴之, 横井彩, 谷口彩乃, 山中玲子, 江國大輔, 森田学

口腔衛生学会雑誌 66 ( 2 ) 219 - 219 2016.4

歯肉炎のスクリーニングにおける唾液中LDH簡易測定キットの有効性

吉田宗生, 加藤壮真, 山根真由, 江國大輔, 横井彩, 丸山貴之, 山中玲子, 友藤孝明, 森田学

口腔衛生学会雑誌 66 ( 2 ) 294 - 294 2016.4

口腔内アセトアルデヒド濃度と舌背の細菌数との関係

加藤壮真, 吉田宗生, 横井彩, 丸山貴之, 江國大輔, 山根真由, 山中玲子, 友藤孝明, 森田学

口腔衛生学会雑誌 66 ( 2 ) 296 - 296 2016.4

歯科衛生士教育における保護者を模擬患者とした実習の取り組みと効果

木村優子, 江國大輔, 森田学, 太田正美

口腔衛生学会雑誌 66 ( 2 ) 245 - 245 2016.4

塩化セチルピリジニウム配合トローチ剤の口臭予防効果

水谷慎介, 友藤孝明, 片岡広太, 川端勇也, 江國大輔, 森田学

日本口臭学会会誌 7 ( 1 ) 54 - 55 2016.4

Relationship Between Prehypertension/Hypertension and Periodontal Disease: A Prospective Cohort Study International journal

Yuya Kawabata, Daisuke Ekuni, Hisataka Miyai, Kota Kataoka, Mayu Yamane, Shinsuke Mizutani, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

AMERICAN JOURNAL OF HYPERTENSION 29 ( 3 ) 388 - 396 2016.3

Language：English Publisher：OXFORD UNIV PRESS

BACKGROUND
Most cross-sectional studies have found a significant positive relationship between periodontal disease and prehypertension/hypertension. However, these studies had limitations and there are few prospective cohort studies in young adults. The purpose of this prospective cohort study was to investigate whether periodontal disease was related to prehypertension/hypertension in Japanese university students.
METHODS
Students (n = 2,588), who underwent health examinations before entering university and before graduation, were included in the analysis. The association between periodontal disease such as the percentage of bleeding on probing (BOP) and community periodontal index (CPI) scores, and change in blood pressure status was determined.
RESULTS
At the reexamination, the numbers of participants with prehypertension (systolic blood pressure 120-139 mm Hg or diastolic blood pressure 80-89 mm Hg) and hypertension (>= 140/90 mm Hg) were 882 (34.1%) and 109 (4.2%), respectively. In a logistic regression model, the risk of hypertension was significantly associated with male (odds ratio (OR): 6.31; 95% confidence interval (CI): 2.63-15.13; P < 0.001), no habitual physical activity at baseline (OR: 2.90; 95% CI: 1.56-5.38; P < 0.01) and periodontal disease defined as the presence of both probing pocket depth (PPD) >= 4 m and BOP >= 30% at baseline (OR: 2.74; 95% CI: 1.19-6.29; P = 0.02) in participants with prehypertension at baseline. On the other hand, the risk of prehypertension was not associated with presence of periodontal disease (OR: 0.93; 95% CI: 0.51-1.70; P = 0.82).
CONCLUSION
In the short-term prospective cohort study, a significant association between presence of periodontal disease and hypertension was observed in Japanese university students.

DOI： 10.1093/ajh/hpv117

Association between Knowledge about Comprehensive Food Education and Increase in Dental Caries in Japanese University Students: A Prospective Cohort Study International journal

Muneyoshi Kunitomo, Daisuke Ekuni, Shinsuke Mizutani, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Mayu Yamane, Kota Kataoka, Ayano Taniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoshiaki Iwasaki, Manabu Morita

NUTRIENTS 8 ( 3 ) 114 - 114 2016.3

Language：English Publisher：MDPI AG

In Japan, comprehensive food education (shokuiku) programs are carried out with the aim of improving dietary practices and thereby reducing the incidence of lifestyle-related diseases, including dental caries. The purpose of this prospective cohort study was to investigate the association between knowledge about shokuiku and the increase in dental caries among Japanese university students who had attended a shokuiku program while in junior/senior high school. A total of 562 students volunteered to undergo oral examinations over a three-year follow-up period, during which the number of cases of dental caries were recorded. Additional information was collected using a questionnaire survey regarding knowledge about shokuiku, dietary habits, and oral health behaviors. In logistic regression analysis, males who lacked knowledge about shokuiku had significantly higher odds for dental caries than those who did not (odds ratio (OR), 2.00; 95% confidence interval (CI), 1.12-3.58; p = 0.019). On the other hand, among females, those who frequently consumed sugar-sweetened soft drinks had significantly higher odds for dental caries than those who did not (OR, 1.89; 95% CI, 1.05-3.42; p = 0.035). These results suggest that having no knowledge about shokuiku is associated with a risk of increase in dental caries in Japanese male university students.

DOI： 10.3390/nu8030114

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