Faculty Profiles - EKUNI Daisuke

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LPS Induced Apoptosis of Periodontal Ligament Fibroblasts in Rats

EKUNI Daisuke, YAMANAKA Reiko, TACHIBANA Kentaro, YAMAMOTO Tatsuo, WATANABE Tatsuo

45 ( 春季特別 ) 126 - 126 2003.4

Proliferative Activity of Epithelial Cells and Fibroblasts by Mechanical Stimulation

YAMANAKA Reiko, EKUNI Daisuke, TOMOFUJI Takaaki, YAMAMOTO Tatsuo, WATANABE Tatsuo

45 ( 春季特別 ) 116 - 116 2003.4

The effect of duration and force of mechanical toothbrushing stimulation on proliferative activity of the junctional epithelium International journal

T Tomofuji, M Morita, M Horiuchi, T Sakamoto, D Ekuni, T Yamamoto, T Watanabe

JOURNAL OF PERIODONTOLOGY 73 ( 10 ) 1149 - 1152 2002.10

Language：English Publisher：AMER ACAD PERIODONTOLOGY

Background: Gingival bleeding is frequently accompanied by an ulcerated epithelium, with repair depending on the proliferative activity of the epithelial cells. The present study examined the proliferative activity of the junctional epithelium (JE) under several different methods of toothbrushing stimulation.
Methods: Twelve dogs were used in this 3-week experiment. Prior to the experiment, all teeth underwent daily removal of plaque and calculus using a scaler. Teeth were then divided into quadrants: 9 teeth in 3 quadrants (test, 3 per quadrant) were mechanically stimulated by toothbrushing for various time intervals (10, 20, or 40 seconds) and at various forces (100, 200, or 250 gf). The 3 teeth in the fourth quadrant served as controls. The proliferative activity of the basal cells of the junctional epithelium was evaluated for expression of proliferating cell nuclear antigen (PCNA). The numbers of PCNA-positive basal cells and total basal cells were counted, and the width of the junctional epithelium was measured.
Results: Toothbrushing force significantly affected the PCNA-positive basal cell ratio (P<0.05). The 200 g toothbrushing force produced the highest PCNA-positive basal cell ratio, which was significantly higher than that using the 100 g force (P <0.05).
Conclusion: In junctional epithelium stimulated with a toothbrush, the PCNA-positive basal cell ratio is more sensitive to toothbrushing force than to duration.

DOI： 10.1902/jop.2002.73.10.1149

ブラッシング頻度の違いによるイヌ歯肉上皮細胞の増殖能の変化

友藤孝明, 江國大輔, 山本龍生, 堀内正純, 坂本友紀, 渡邊達夫

口腔衛生学会雑誌 52 ( 5 ) 723 - 724 2002.10

TUNEL-positive Cells and Apical Migration of Junctional Epithelium by LPS and Protease

EKUNI Daisuke, YAMANAKA Reiko, TACHIBANA Kentaro, YAMAMOTO Tatsuo, WATANABE Tatsuo

Journal of the Japanese Association of Periodontology 44 ( 秋季特別 ) 162 - 162 2002.9

ブラッシングによって応答するイヌ歯肉PCNA陽性細胞の分布

江國大輔, 友藤孝明, 坂本友紀, 堀内正純, 山本龍生, 渡邊達夫

口腔衛生学会雑誌 52 ( 4 ) 552 - 553 2002.8

Comparison of the Sonic Electric Toothbrush and the Oscillating/rotating Electric Toothbrush on Dog's Gingival Fibroblast Proliferation

Tomofuji Takaaki, Ekuni Daisuke, Yamamoto Tatuso, Horiuchi Masazumi, Sakamoto Tomonori, Watanabe Tatsuo

Journal of the Japanese Association of Periodontology 44 ( 春季特別 ) 126 - 126 2002.3

Comparison of gingival fibroblast proliferation with two electric toothbrushes.

T Tomofuji, D Ekuni, T Sakamoto, N Horiuchi, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH 81 A374 - A374 2002.3

PCNA positive cells in apical migration of rat's junctional epithelium.

T Yamamoto, D Ekuni, R Yamanaka, K Tachibana, T Watanabe

JOURNAL OF DENTAL RESEARCH 81 A372 - A372 2002.3

Apical migration of junctional epithelium by lipopolysaccharide and protease.

D Ekuni, R Yamanaka, K Tachibana, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH 81 A206 - A206 2002.3

ナイロンに多元素鉱物粉状体を混合して形成したフィラメントを用いた歯ブラシのブラッシング効果.

坂本友紀, 友藤孝明, 堀内正純, 江國大輔, 森田学, 山本龍生, 渡邊達夫

口腔衛生学会雑誌 2002

歯周病原因子を投与することによってラットに歯周炎を誘発するモデルの研究

江國大輔

岡山歯学会雑誌 2002

ブラッシング頻度の違いによるイヌ歯肉線維芽細胞の活性化

友藤孝明, 江國大輔, 坂本友紀, 堀内正純, 山本龍生, 渡邊達夫

口腔衛生学会雑誌 51 ( 4 ) 552 - 553 2001.9

Reduction of inflammatory cell infiltration by mechanical stimulation of toothbrushing.

T Sakamoto, D Ekuni, T Tomofuji, M Horiuchi, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH 80 ( 4 ) 1343 - 1343 2001.4

Activation of Dogs Gingival Fibroblasts depends on Toothbrushing Frequency

TOMOFUJI T., EKUNI D., SAKAMOTO T., HORIUCHI M., YAMAMOTO T., WATANABE T.

Journal of the Japanese Association of Periodontology 43 ( 春季特別 ) 101 - 101 2001.3

ブラッシングによるイヌ歯肉組織の応答範囲

坂本友紀, 友藤孝明, 江國大輔, 堀内正純, 山本龍生, 渡邊達夫

口腔衛生学会雑誌 50 ( 5 ) 834 - 834 2000.10

Effect of Continuous Application of E. coli LPS on Proliferating Activity of Rats' Junctional Epithelium

EKUNI Daisuke, YAMAMOTO Tatsuo, WATANABE Tatsuo

Journal of the Japanese Association of Periodontology 42 ( 秋季特別 ) 174 - 174 2000.9

ブラッシング力と時間の違いによるイヌ歯肉炎症性細菌浸潤の変化

友藤孝明, 山本龍生, 堀内正純, 坂本友紀, 江國大輔, 渡邊達夫

口腔衛生学会雑誌 50 ( 4 ) 564 - 565 2000.9

Location of PCNA positive gingival cells by toothbrushing.

T Tomofuji, D Ekuni, T Sakamoto, M Horiuchi, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH 79 ( 5 ) 1239 - 1239 2000.5

Immunohistochemical Analysis of Dogs' Gingiva baced on Toothbrushing Force

TOMOFUJI Takaaki, EKUNI Daisuke, SAKAMOTO Tomonori, HORIUCHI Masazumi, YAMAMOTO Tatsuo, WATANABE Tatsuo

Journal of the Japanese Association of Periodontology 42 ( 春季特別 ) 106 - 106 2000.4

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