Faculty Profiles - NISHIDA Keiichiro

Papers - NISHIDA Keiichiro

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島村安則, 今谷潤也, 野田知之, 中原龍一, 齋藤太一, 金澤智子, 井上円加, 小澤正嗣, 古松毅之, 国定俊之, 西田圭一郎, 橋詰博行, 尾崎敏文

日本整形外科学会雑誌 85 ( 8 ) S1338 - S1338 2011.8

清水弘毅, 今谷潤也, 竹下歩, 堀田昌宏, 大塚亮介, 中道亮, 山川泰明, 川上幸雄, 林正典, 西田圭一郎

骨折 33 ( Suppl. ) S55 - S55 2011.7

[Cutting edge on research of cartilage metabolism. Recent progress in bio-molecular imaging of articular cartilage]. Reviewed

Toshitaka Oohashi, Keiichiro Nishida

Clinical calcium 21 ( 6 ) 896 - 902 2011.6

Language：Japanese Publishing type：Research paper (scientific journal)

In the process of cartilage degeneration seen in osteoarthritis, loss of proteoglycan from articular cartilage has been widely accepted as a critical early event, followed by collagen degradation designated as a point of no return. Recent advance in the development of targeted molecular probes and new imaging modalities enabled the detection of qualitative and functional change of articular cartilage. In this paper, we describe the recent progress of bio-molecular imaging of articular cartilage including our fluorescent-labeled glycosaminoglycan-binding octaarginine.

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ENU-induced missense mutation in the C-propeptide coding region of Col2a1 creates a mouse model of platyspondylic lethal skeletal dysplasia, Torrance type

Tatsuya Furuichi, Hiroshi Masuya, Tomohiko Murakami, Keiichiro Nishida, Gen Nishimura, Tomohiro Suzuki, Kazunori Imaizumi, Takashi Kudo, Kiyoshi Ohkawa, Shigeharu Wakana, Shiro Ikegawa

MAMMALIAN GENOME 22 ( 5-6 ) 318 - 328 2011.6

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

The COL2A1 gene encodes the alpha 1(II) chain of the homotrimeric type II collagen, the most abundant protein in cartilage. In humans, COL2A1 mutations create many clinical phenotypes collectively termed type II collagenopathies; however, the genetic basis of the phenotypic diversity is not well elucidated. Therefore, animal models corresponding to multiple type II collagenopathies are required. In this study we identified a novel Col2a1 missense mutation-c.44406A > C (p.D1469A)-produced by large-scale N-ethyl-N-nitrosourea (ENU) mutagenesis in a mouse line. This mutation was located in the C-propeptide coding region of Col2a1 and in the positions corresponding to a human Col2a1 mutation responsible for platyspondylic lethal skeletal dysplasia, Torrance type (PLSD-T). The phenotype was inherited as a semidominant trait. The heterozygotes were mildly but significantly smaller than wild-type mice. The homozygotes exhibited lethal skeletal dysplasias, including extremely short limbs, severe spondylar dysplasia, severe pelvic hypoplasia, and brachydactyly. As expected, these skeletal defects in the homozygotes were similar to those in PLSD-T patients. The secretion of the mutant proteins into the extracellular space was disrupted, accompanied by abnormally expanded rough endoplasmic reticulum (ER) and upregulation of ER stress-related genes, such as Grp94 and Chop, in chondrocytes. These findings suggested that the accumulation of mutant type II collagen in the ER and subsequent induction of ER stress are involved, at least in part in the PLSD-T-like phenotypes of the mutants. This mutant should serve as a good model for studying PLSD-T pathogenesis and the mechanisms that create the great diversity of type II collagenopathies.

DOI： 10.1007/s00335-011-9329-3

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生物学的製剤と外科治療生物学的製剤使用患者の手術のタイミング

西田圭一郎, 金澤智子, 中原龍一, 橋詰謙三, 那須義久, 斎藤太一, 尾崎敏文

日本リウマチ学会総会・学術集会・国際リウマチシンポジウムプログラム・抄録集 55回・20回 147 - 147 2011.6

関節リウマチの手術上肢関節リウマチ(RA)に対するSauve-Kapandji法の治療成績

金澤智子, 橋詰謙三, 西田圭一郎, 中原龍一, 斉藤太一, 尾崎敏文

日本リウマチ学会総会・学術集会・国際リウマチシンポジウムプログラム・抄録集 55回・20回 383 - 383 2011.6

関節リウマチの手術上肢 RA手関節に対する部分固定術の術後成績

斉藤太一, 西田圭一郎, 橋詰謙三, 中原龍一, 金澤智子, 尾崎敏文

日本リウマチ学会総会・学術集会・国際リウマチシンポジウムプログラム・抄録集 55回・20回 312 - 312 2011.6

関節リウマチの手術上肢関節リウマチ手指MP関節に対する人工指関節置換術の成績

橋詰謙三, 西田圭一郎, 那須義久, 中原龍一, 斉藤太一, 金澤智子, 尾崎敏文

日本リウマチ学会総会・学術集会・国際リウマチシンポジウムプログラム・抄録集 55回・20回 310 - 310 2011.6

炎症標的化金コロイド内包リポソームのリウマチ関節炎症部位への集積

古谷満寿美, 松本衣未, 美名口順, 小川弘子, 古松毅之, 廣畑聡, 二宮善文, 西田圭一郎, 大塚愛二, 大橋俊孝

日本結合組織学会学術大会・マトリックス研究会大会合同学術集会プログラム・抄録集 43回・58回 118 - 118 2011.5

Increased level of heme oxygenase-1 in rheumatoid arthritis synovial fluid

Ai Kitamura, Keiichiro Nishida, Takamitsu Komiyama, Hideyuki Doi, Yasutaka Kadota, Aki Yoshida, Toshifumi Ozaki

MODERN RHEUMATOLOGY 21 ( 2 ) 150 - 157 2011.4

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

We investigated the expression and localization of heme oxygenase-1 (HO-1) in synovial fluid and synovial tissue, and examined the stimulation of HO-1 production in rheumatoid synovial fibroblasts (RASFs). Synovial fluid samples were obtained from knee joints of 20 rheumatoid arthritis (RA) and 20 osteoarthritis (OA) patients, and concentration of HO-1 and matrix metalloproteinase-3 (MMP-3) were measured by enzyme-linked immunosorbent assay (ELISA). Synovial tissues obtained from RA or OA patients during total knee arthroplasty (TKA) were used for immunohistochemical analysis of HO-1. HO-1 production by RASFs in response to various cytokines was examined by ELISA. HO-1 levels in synovial fluid were higher in the RA group than in the OA group with significant difference (P < 0.001), and correlated with serum C-reactive protein (CRP) level (r = 0.80, P < 0.01) in the RA group. Higher levels of HO-1 were seen in the RA-L group (Larsen grade III-V) than in the RA-E (Larsen grade 0-II) group (P < 0.001). There was weak correlation between the levels of HO-1 protein and MMP-3 in synovial fluid in the RA group (r = 0.31, P < 0.01), while no positive correlation was observed in OA. Positive immunoreaction for HO-1 was observed in cells of synovial tissue including synovial fibroblasts and cells in synovial pannus. HO-1 protein levels in cultured media of RASFs were increased by stimulation by interleukin-1 beta at 6 h and tumor necrosis factor-alpha at 12 h, but suppressed by interferon-gamma at 12 and 24 h. These results indicated that HO-1 expression in synovial tissue might be stimulated by inflammatory cytokines. The correlation of HO-1 concentration in synovial fluid with serum CRP and MMP-3 in joint fluid indicated that HO-1 might be useful as a marker of joint inflammation in RA patients.

DOI： 10.1007/s10165-010-0372-9

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ハンドル外傷による舟状骨骨折の1例

金澤智子, 島村安則, 西田圭一郎, 斎藤太一, 中原龍一, 雑賀建多, 野田知之, 尾崎敏文

中国・四国整形外科学会雑誌 23 ( 1 ) 253 - 253 2011.4

掌側プレート固定を行った橈骨遠位端骨折における抜釘術の重要性

島村安則, 井上円加, 小澤正嗣, 斎藤太一, 金澤智子, 中原龍一, 雑賀建多, 野田知之, 西田圭一郎, 尾崎敏文

中国・四国整形外科学会雑誌 23 ( 1 ) 258 - 258 2011.4

RA手関節に対する部分固定術の術後成績

斎藤太一, 西田圭一郎, 橋詰謙三, 島村安則, 尾崎敏文

日本手外科学会雑誌 28 ( 1 ) S138 - S138 2011.3

ANTERIOR TRANSPOSITION OF THE ULNAR NERVE WITH ENDOSCOPIC ASSISTANCE

T. Konishiike, K. Nishida, M. Ozawa, T. Ozaki

JOURNAL OF HAND SURGERY-EUROPEAN VOLUME 36E ( 2 ) 126 - 129 2011.2

Language：English Publishing type：Research paper (scientific journal) Publisher：SAGE PUBLICATIONS LTD

We treated 20 patients with cubital tunnel syndrome by anterior transposition of the ulnar nerve with endoscopic assistance. Five elbows were classified preoperatively as McGowan's stage 1, 11 as stage 2 and four as stage 3. Excellent outcomes were obtained in nine and good in eight patients. Three patients had fair results. Improvement of symptoms occurred in all patients. There were no serious complications. All ulnar nerves remained anteriorly transposed.

DOI： 10.1177/1753193410381675

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Prenatal diagnosis of short-rib polydactyly syndrome type 3 (Verma-Naumoff type) by three-dimensional helical computed tomography

Takahiro Yamada, Gen Nishimura, Keiichiro Nishida, Hideaki Sawai, Tokuhiko Omatsu, Taichi Kimura, Hiroshi Nishihara, Rina Shono, Shigeki Shimada, Mamoru Morikawa, Masato Mizushima, Takashi Yamada, Kazutoshi Cho, Shinya Tanaka, Hiroki Shirato, Hisanori Minakami

JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH 37 ( 2 ) 151 - 155 2011.2

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL PUBLISHING, INC

We present a case of short-rib polydactyly syndrome (SRPs) type 3 in which accurate prenatal diagnosis was feasible using both ultrasonography and 3D-CT. SRP encompass a heterogeneous group of lethal skeletal dysplasias. However, the phenotypes overlap with those of nonlethal skeletal dysplasias (i.e. Ellis-van Creveld syndrome and Jeune syndrome). As accurate prenatal diagnosis of SRP is helpful for parents, we used 3D-CT in the early third trimester to examine a fetus suggested to have phenotypes of 'short-rib dysplasia group' on ultrasonography. 3D-CT showed mild modification of the vertebral bodies, small ilia with horizontal acetabula and triangular partial ossification defects, and subtle metaphyseal irregularities of the femora. These CT findings and an extensive literature search regarding the phenotypes of various diseases categorized as short-rib dysplasia group led to a correct prenatal diagnosis of SRP type 3. This case exemplified the usefulness of 3D-CT for the precise prenatal diagnosis of skeletal dysplasias.

DOI： 10.1111/j.1447-0756.2010.01324.x

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Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells

T. Tetsunaga, K. Nishida, T. Furumatsu, K. Naruse, S. Hirohata, A. Yoshida, T. Saito, T. Ozaki

OSTEOARTHRITIS AND CARTILAGE 19 ( 2 ) 222 - 232 2011.2

Language：English Publishing type：Research paper (scientific journal) Publisher：W B SAUNDERS CO LTD

Objective: To investigate the mechanism of mechanical stress-induced expression and regulation of aggrecanases and examine the role of runt-related transcription factor 2 (RUNX-2) in chondrocyte-like cells.
Methods: SW1353 cells were seeded onto stretch chambers at a concentration of 5 x 10(4) cells/chamber, and a uni-axial cyclic tensile strain (CTS) (0.5 Hz, 10% stretch) was applied for 30 min. Total RNA was extracted, reverse transcribed, and analyzed by polymerase chain reaction (PCR) and real-time PCR. RUNX-2 overexpression and small interfering RNA (siRNA) targeting RUNX-2 were used to investigate the role of RUNX-2 in CTS-induced gene expression. The involvement of diverse mitogen-activated protein kinase (MAPK) pathways in the activation of RUNX-2, MMP-13 and ADAMTS-5 during CTS was examined by Western blotting.
Results: CTS induced expression of RUNX-2, MMP-13, ADAMTS-4, -5, and -9. Overexpression of RUNX-2 up-regulated expression of MMP-13 and ADAMTS-5, whereas RUNX-2 siRNA resulted in significant down-regulation of mechanically-induced MMP-13 and ADAMTS-5 expression. CTS induced activation of p38 MAPK, and CTS induction of RUNX-2. MMP-13 and ADAMTS-5 mRNA was down-regulated by the selective p38 MAPK inhibitor SB203580 but not by the p44/42 MAPK inhibitor U0126, or the JNK MAPK inhibitor JNK inhibitor II.
Conclusions: RUNX-2 might have a role as a key downstream mediator of p38's ability to regulate mechanical stress-induced MMP-13 and ADAMTS-5 expression. (C) 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.joca.2010.11.004

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Well leg compartment syndrome following radical cystectomy: A case report

OKADA Yoshiki

The Central Japan Journal of Orthopaedic Surgery & Traumatology 54 ( 4 ) 869 - 870 2011

Time-concentration profile of serum etanercept in Japanese patients with rheumatoid arthritis after treatment discontinuation before orthopedic surgery

Keiichiro Nishida, Kenzo Hashizume, Yasutaka Kadota, Masamitsu Natsumeda, Ryuichi Nakahara, Taichi Saito, Tomoko Kanazawa, Kazuhiko Ezawa, Toshihumi Ozaki

MODERN RHEUMATOLOGY 20 ( 6 ) 637 - 639 2010.12

長橈側手根伸筋(ECRL)腱を用いて母指手根中手骨(CM)関節形成術を行った1例

井上円加, 西田圭一郎, 橋詰謙三, 島村安則, 中原龍一, 斉藤太一, 金澤智子, 尾崎敏文

岡山医学会雑誌 122 ( 3 ) 279 - 279 2010.12

【関節リウマチ明確な治療目標の達成をめざす診療】パラダイムシフト後の関節外科治療

那須義久, 中原龍一, 西田圭一郎

治療学 44 ( 10 ) 1127 - 1130 2010.10

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Papers - NISHIDA Keiichiro