研究者詳細 - 松本　卓也

論文 - 松本　卓也

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二官能メタクリレートの添加が即時重合レジンの曲げ特性に与える影響

山本美恵, 丸尾幸憲, 入江正郎, 西川悟郎, 玉田宜之, 松本卓也, 皆木省吾

日本歯科理工学会誌 31 ( 5 ) 452 - 452 2012年9月

即時重合レジンの重合収縮の計測方法に関する研究

丸尾幸憲, 山本美恵, 入江正郎, 西川悟郎, 玉田宜之, 松本卓也, 皆木省吾

日本歯科理工学会誌 31 ( 5 ) 458 - 458 2012年9月

Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis

Kenichi Harimoto, Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Yoh-ichi Tagawa

DENTAL MATERIALS JOURNAL 31 ( 4 ) 674 - 680 2012年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Titanium has an osseointegrative property, while hydroxyapatite has an osteoconductive property. It remains a matter of controversy among researchers whether hydroxyapatite has higher osteoblast compatibility than titanium. Here, we compared the activities between osteoblasts cultured on titanium and those cultured on hydroxyapatite. An osteoblast-like cell line, MC3T3-E1, was cultured on machined titanium, evaporated titanium, and hydroxyapatite disks to compare the affinity of osteoblasts to each of these materials. The adhesion and proliferation of MC3T3-E1 cells were higher on hydroxyapatite disks than on the other disks. Osteoblast differentiation was not affected by the nature of disks investigated, but calcium was more easily deposited on the hydroxyapatite disks. The amount of absorbed serum proteins detected on hydroxyapatite was greater than that on titanium. In conclusion, our results indicate hydroxyapatite is a more suitable material for osteoblast growth than titanium because of its higher absorption of serum proteins.

DOI： 10.4012/dmj.2012-075

X-ray diffraction analysis of three-dimensional self-reinforcing monomer and its chemical interaction with tooth and hydroxyapatite 査読

Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Masao Hanabusa, Takuya Matsumoto, Yasuko Momoi

DENTAL MATERIALS JOURNAL 31 ( 4 ) 697 - 702 2012年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

According to the 'Adhesion-Decalcification' concept, specific functional monomers within dental adhesives can ionically interact with hydroxyapatite (HAp). Some specific functional monomers form monomer-Ca salts due to chemical interactions. The chemical stability of the monomer-Ca salts was thought to contribute to bond durability. In the present study, we analyzed the chemical interaction between an acidic three-dimensional self-reinforcing monomer (3D-SR) of Bond Force and enamel, dentin and HAp, and assessed its chemical stability by thin-film X-ray diffraction (TF-XRD). 3D-SR forms a hydrolysis-resistant Ca-salt on the dentin in a clinical application time period and on enamel and HAp in a longer time period. This suggests that the functional monomer 3D-SR is able to contribute to bond durability.

DOI： 10.4012/dmj.2012-074

Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis 査読

Kenichi Harimoto, Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Yoh-ichi Tagawa

DENTAL MATERIALS JOURNAL 31 ( 4 ) 674 - 680 2012年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Titanium has an osseointegrative property, while hydroxyapatite has an osteoconductive property. It remains a matter of controversy among researchers whether hydroxyapatite has higher osteoblast compatibility than titanium. Here, we compared the activities between osteoblasts cultured on titanium and those cultured on hydroxyapatite. An osteoblast-like cell line, MC3T3-E1, was cultured on machined titanium, evaporated titanium, and hydroxyapatite disks to compare the affinity of osteoblasts to each of these materials. The adhesion and proliferation of MC3T3-E1 cells were higher on hydroxyapatite disks than on the other disks. Osteoblast differentiation was not affected by the nature of disks investigated, but calcium was more easily deposited on the hydroxyapatite disks. The amount of absorbed serum proteins detected on hydroxyapatite was greater than that on titanium. In conclusion, our results indicate hydroxyapatite is a more suitable material for osteoblast growth than titanium because of its higher absorption of serum proteins.

DOI： 10.4012/dmj.2012-075

Surface characterization of alkali- and heat-treated Ti with or without prior acid etching

Sang-Hyun An, Takuya Matsumoto, Hiroyuki Miyajima, Jun-Ichi Sasaki, Ramaswamy Narayanan, Kyo-Han Kim

APPLIED SURFACE SCIENCE 258 ( 10 ) 4377 - 4382 2012年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Titanium and its alloys are used as implant materials in dental and orthopaedic applications. The material affinities to host bone tissue greatly concern with the recovery period and good prognosis. To obtain a material surface having excellent affinity to bone, acid etching prior to alkali- and heat-treatment of Ti was conducted. The surface characteristics of the prepared sample indicated that the roughness as well as the wettability increased by pre-etching. Bone-like apatite was formed on pre-etched, alkali- and heat-treated Ti surface in simulated body fluid (SBF) within 3 days, while it takes 5 days on the solely alkali- and heat-treated surface. Osteoblastic cells showed better compatibility on the per-etched surface compared to the pure Ti surface or alkali- and heat-treated surface. Moreover, the pre-etched surface showed better pull-off tensile adhesion strength against the deposited apatite. Thus, acid etching prior to alkali- and heat-treatment would be a promising method for enhancing the affinity of Ti to host bone tissue. (C) 2012 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.apsusc.2011.12.119

ジルコニア表面のリン酸モノマーによる有機化の検討

玉田宜之, 長岡紀幸, 早川聡, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 松本卓也, 尾坂明義, 皆木省吾

日本歯科理工学会誌 31 ( 2 ) 190 - 190 2012年3月

In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct 査読

Jun-Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Michiya Matsusaki, Akihiro Nishiguchi, Takayoshi Nakano, Mitsuru Akashi, Satoshi Imazato, Hirofumi Yatani

INTEGRATIVE BIOLOGY 4 ( 10 ) 1207 - 1214 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ROYAL SOC CHEMISTRY

Endochondral ossification is one of the essential bone development processes in vertebrates. Although researchers from a variety of fields, including cellular/molecular biology, chemistry, and materials science, have worked to gain a better understanding of the tissue development, integration of findings from these different fields remains a major challenge. An in vitro model system that reproduces endochondral ossification would be a valuable tool for overcoming this problem, because an in vitro standardized model system can be easily accessed by researchers from different fields. Here, we fabricated a large 3D mesenchymal stem cell (MSC) construct with a ball-like morphology, which is termed a cell ball, and cultured it under a hypoxia condition, since hypoxia causes chondrogenic differentiation of MSCs in primordial cartilage, which is crucial for endochondral ossification. Region-specific chondrogenic differentiation of MSCs and mineralization within the cartilage tissue were observed in the cell ball. The precipitated minerals were detected as hydroxyapatite. Consequently, a 3D construct consisting of mineralized tissue surrounded by cartilage tissue was obtained. Moreover, the angiogenic activity of this synthesized tissue changed depending on the chondrogenic phenotype remains in the tissue, which is similar to what happens in the ossification process. Thus, this MSC cell ball system clearly reproduced the initial stage of endochondral ossification in vitro. This system is a promising tool for use as an in vitro model for investigating bone tissue development.

DOI： 10.1039/c2ib20027a

Zirconia-hydroxyapatite composite material with micro porous structure 査読

Takuya Junior Matsumoto, Sang-Hyun An, Takuya Ishimoto, Takayoshi Nakano, Takuya Matsumoto, Satoshi Imazato

DENTAL MATERIALS 27 ( 11 ) E205 - E212 2011年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Objectives. Titanium plates and apatite blocks are commonly used for restoring large osseous defects in dental and orthopedic surgery. However, several cases of allergies against titanium have been recently reported. Also, sintered apatite block does not possess sufficient mechanical strength. In this study, we attempted to fabricate a composite material that has mechanical properties similar to biocortical bone and high bioaffinity by compounding hydroxyapatite (HAp) with the base material zirconia (ZrO2), which possesses high mechanical properties and low toxicity toward living organisms.
Methods. After mixing the raw material powders at several different ZrO2/HAp mixing ratios, the material was compressed in a metal mold (8 mm in diameter) at 5 MPa. Subsequently, it was sintered for 5 h at 1500 degrees C to obtain the ZrO2/HAp composite. The mechanical property and biocompatibility of materials were investigated. Furthermore, osteoconductivity of materials was investigated by animal studies.
Results. A composite material with a minute porous structure was successfully created using ZrO2/HAp powders, having different particle sizes, as the starting material. The material also showed high protein adsorption and a favorable cellular affinity. When the mixing ratio was ZrO2/HAp = 70/30, the strength was equal to cortical bone. Furthermore, in vivo experiments confirmed its high osteoconductivity.
Significance. The composite material had strength similar to biocortical bones with high cell and tissue affinities by compounding ZrO2 and HAp. The ZrO2/HAp composite material having micro porous structure would be a promising bone restorative material. (C) 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.dental.2011.07.009

Zirconia-hydroxyapatite composite material with micro porous structure

Takuya Junior Matsumoto, Sang-Hyun An, Takuya Ishimoto, Takayoshi Nakano, Takuya Matsumoto, Satoshi Imazato

DENTAL MATERIALS 27 ( 11 ) E205 - E212 2011年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Objectives. Titanium plates and apatite blocks are commonly used for restoring large osseous defects in dental and orthopedic surgery. However, several cases of allergies against titanium have been recently reported. Also, sintered apatite block does not possess sufficient mechanical strength. In this study, we attempted to fabricate a composite material that has mechanical properties similar to biocortical bone and high bioaffinity by compounding hydroxyapatite (HAp) with the base material zirconia (ZrO2), which possesses high mechanical properties and low toxicity toward living organisms.
Methods. After mixing the raw material powders at several different ZrO2/HAp mixing ratios, the material was compressed in a metal mold (8 mm in diameter) at 5 MPa. Subsequently, it was sintered for 5 h at 1500 degrees C to obtain the ZrO2/HAp composite. The mechanical property and biocompatibility of materials were investigated. Furthermore, osteoconductivity of materials was investigated by animal studies.
Results. A composite material with a minute porous structure was successfully created using ZrO2/HAp powders, having different particle sizes, as the starting material. The material also showed high protein adsorption and a favorable cellular affinity. When the mixing ratio was ZrO2/HAp = 70/30, the strength was equal to cortical bone. Furthermore, in vivo experiments confirmed its high osteoconductivity.
Significance. The composite material had strength similar to biocortical bones with high cell and tissue affinities by compounding ZrO2 and HAp. The ZrO2/HAp composite material having micro porous structure would be a promising bone restorative material. (C) 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.dental.2011.07.009

Hydrogel-based biomimetic environment for in vitro modulation of branching morphogenesis. 査読

Miyajima H, Matsumoto T, Sakai T, Yamaguchi S, An SH, Abe M, Wakisaka S, Lee KY, Egusa H, Imazato S

Biomaterials 32 ( 28 ) 6754 - 6763 2011年10月

Crystallinity of Anodic TiO(2) Nanotubes and Bioactivity

Sang-Hyun An, Ramaswamy Narayanan, Takuya Matsumoto, Hyo-Jin Lee, Tae-Yub Kwon, Kyo-Han Kim

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 11 ( 6 ) 4910 - 4918 2011年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SCIENTIFIC PUBLISHERS

Anodic TiO(2) nanotubes were produced on titanium at 20 V using 1 M Na(2)SO(4) and 0.5 wt% NaF. Oxidation for 3 hours produced amorphous tubes of diameter 100 nm and thicknesses 2 mu m. Heat-treatments were done for 3 hours at different temperatures. 300 degrees C treatment converted the amorphous coatings to anatase. 550 and 700 degrees C treatments formed dual anatase and rutile; 850 degrees C treatment crystallized to rutile. The treatment at 700 degrees C produced an oxide surface with higher roughness, lower wetting angle and higher coating adhesion. Bioactivity of the as-oxidized and heated coatings were evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the dual oxide structure within 3 days. HA was detected after 7 days in the anatase structure and only after 21 days in the amorphous and rutile structures. In vitro cell culture tests done using mouse osteoblasts indicated that, the 700 degrees C-heated surface showed higher levels of cell activity than the other surfaces. It is concluded that the dual rutile and anatase structure formed by heating the oxide at 700 degrees C is the best of the five surfaces tested.

DOI： 10.1166/jnn.2011.4114

Cell-Based Fabrication of Organic/Inorganic Composite Gel Material 査読

Takuya Matsumoto, Ami Mizuno, Miki Kashiwagi, Shin-suke Yoshida, Jun-ichi Sasaki, Takayoshi Nakano

MATERIALS 4 ( 1 ) 327 - 338 2011年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Biomaterials containing components similar to the native biological tissue would have benefits as an implantable scaffold material. To obtain such biomimetic materials, cells may be great contributors because of their crucial roles in synthetic organics. In addition, the synthesized organics-especially those derived from osteogenic differentiated cells-become a place where mineral crystals nucleate and grow even in vitro. Therefore, to fabricate an organic/inorganic composite material, which is similar to the biological osteoid tissue, bone marrow derived mesenchymal stem cells (BMSCs) were cultured in a 3D fibrin gel in this study. BMSCs secreted bone-related proteins that enhanced the biomineralization within the gel when the cells were cultured with an osteogenic differentiation medium. The compositions of both synthesized matrices and precipitated minerals in the obtained materials altered depending on the cell culture period. The mineral obtained in the 3D gel showed low crystalline hydroxyapatite. The composite materials also showed excellent osteoconductivity with new bone formation when implanted in mice tibiae. Thus, we demonstrated the contributions of cells for fabricating implantable organic/inorganic composite gel materials and a method for controlling the material composition in the gel. This cell-based material fabrication method would be a novel method to fabricate organic/inorganic composite biomimetic materials for bone tissue engineering.

DOI： 10.3390/ma4010327

Modulation of nanotube formation in apatite single crystal via organic molecule incorporation

Matsumoto, T., Uddin, M.H., An, S.H., Arakawa, K., Taguchi, E., Nakahira, A., Okazaki, M.

Materials Chemistry and Physics 128 ( 3 ) 455 - 499 2011年

Gingival Fibroblasts as a Promising Source of Induced Pluripotent Stem Cells 査読

Hiroshi Egusa, Keisuke Okita, Hiroki Kayashima, Guannan Yu, Sho Fukuyasu, Makio Saeki, Takuya Matsumoto, Shinya Yamanaka, Hirofumi Yatani

PLOS ONE 5 ( 9 ) e12743 2010年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PUBLIC LIBRARY SCIENCE

Background: Induced pluripotent stem (iPS) cells efficiently generated from accessible tissues have the potential for clinical applications. Oral gingiva, which is often resected during general dental treatments and treated as biomedical waste, is an easily obtainable tissue, and cells can be isolated from patients with minimal discomfort.
Methodology/Principal Findings: We herein demonstrate iPS cell generation from adult wild-type mouse gingival fibroblasts (GFs) via introduction of four factors (Oct3/4, Sox2, Klf4 and c-Myc; GF-iPS-4F cells) or three factors (the same as GF-iPS-4F cells, but without the c-Myc oncogene; GF-iPS-3F cells) without drug selection. iPS cells were also generated from primary human gingival fibroblasts via four-factor transduction. These cells exhibited the morphology and growth properties of embryonic stem (ES) cells and expressed ES cell marker genes, with a decreased CpG methylation ratio in promoter regions of Nanog and Oct3/4. Additionally, teratoma formation assays showed ES cell-like derivation of cells and tissues representative of all three germ layers. In comparison to mouse GF-iPS-4F cells, GF-iPS-3F cells showed consistently more ES cell-like characteristics in terms of DNA methylation status and gene expression, although the reprogramming process was substantially delayed and the overall efficiency was also reduced. When transplanted into blastocysts, GF-iPS-3F cells gave rise to chimeras and contributed to the development of the germline. Notably, the four-factor reprogramming efficiency of mouse GFs was more than 7-fold higher than that of fibroblasts from tail-tips, possibly because of their high proliferative capacity.
Conclusions/Significance: These results suggest that GFs from the easily obtainable gingival tissues can be readily reprogrammed into iPS cells, thus making them a promising cell source for investigating the basis of cellular reprogramming and pluripotency for future clinical applications. In addition, high-quality iPS cells were generated from mouse GFs without Myc transduction or a specific system for reprogrammed cell selection.

DOI： 10.1371/journal.pone.0012743

Fabrication of Three-Dimensional Cell Constructs Using Temperature-Responsive Hydrogel 査読

Jun-Ichi Sasaki, Taka-Aki Asoh, Takuya Matsumoto, Hiroshi Egusa, Taiji Sohmura, Eben Alsberg, Mitsuru Akashi, Hirofumi Yatani

TISSUE ENGINEERING PART A 16 ( 8 ) 2497 - 2504 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.

DOI： 10.1089/ten.tea.2009.0523

Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts 査読

Shinya Nakamura, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroshi Egusa, Kuen Yong Lee, Takayoshi Nakano, Taiji Sohmura, Atsushi Nakahira

TISSUE ENGINEERING PART A 16 ( 8 ) 2467 - 2473 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT INC

Apatite biomaterials have potential not only as cell vehicles for engineering bone tissue but also as regulators of calcium (Ca) concentration in situ for controlling osteoblast functions, for example, osteogenic differentiation and fate management of hematopoietic stem cells (HSCs). To design apatite materials having optimal chemical properties for the latter purpose, more detailed investigations into what effect Ca concentrations have on osteoblast functions is crucial. In this study, osteoblasts were cultured at different Ca concentrations, and the temporal alterations in osteogenic differentiation and HSC niche-related protein (angiopoietin-1, 2 [Ang1, 2]) expression were investigated. The different Ca concentrations (1.8-50 mmol/L) in the cell culture medium had no effect on the proliferation of osteoblasts, but did on the cell morphology. The higher Ca concentrations (<6 mmol/L) enhanced the mineralization as well as Ang1 expression. In addition, Ang1 expression in osteoblasts showed higher correlation with expression of connexin43, the major marker of cell-cell interactions, whereas Ang2 related to integrin beta 1, the major marker of cell-matrix interactions. Thus, the local Ca concentration regulates cell morphology through the cell-cell or cell-matrix interactions, leading to the alteration of Ang1 expression in osteoblasts. Since these changes triggered by Ca are concerned with the osteogenic differentiation or reproduction of HSCs niche microenvironment, the results obtained in this study might be useful for designing apatite materials with optimal chemical properties.

DOI： 10.1089/ten.tea.2009.0337

Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts 査読

Shinya Nakamura, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroshi Egusa, Kuen Yong Lee, Takayoshi Nakano, Taiji Sohmura, Atsushi Nakahira

TISSUE ENGINEERING PART A 16 ( 8 ) 2467 - 2473 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT INC

Apatite biomaterials have potential not only as cell vehicles for engineering bone tissue but also as regulators of calcium (Ca) concentration in situ for controlling osteoblast functions, for example, osteogenic differentiation and fate management of hematopoietic stem cells (HSCs). To design apatite materials having optimal chemical properties for the latter purpose, more detailed investigations into what effect Ca concentrations have on osteoblast functions is crucial. In this study, osteoblasts were cultured at different Ca concentrations, and the temporal alterations in osteogenic differentiation and HSC niche-related protein (angiopoietin-1, 2 [Ang1, 2]) expression were investigated. The different Ca concentrations (1.8-50 mmol/L) in the cell culture medium had no effect on the proliferation of osteoblasts, but did on the cell morphology. The higher Ca concentrations (<6 mmol/L) enhanced the mineralization as well as Ang1 expression. In addition, Ang1 expression in osteoblasts showed higher correlation with expression of connexin43, the major marker of cell-cell interactions, whereas Ang2 related to integrin beta 1, the major marker of cell-matrix interactions. Thus, the local Ca concentration regulates cell morphology through the cell-cell or cell-matrix interactions, leading to the alteration of Ang1 expression in osteoblasts. Since these changes triggered by Ca are concerned with the osteogenic differentiation or reproduction of HSCs niche microenvironment, the results obtained in this study might be useful for designing apatite materials with optimal chemical properties.

DOI： 10.1089/ten.tea.2009.0337

Fabrication of Three-Dimensional Cell Constructs Using Temperature-Responsive Hydrogel 査読

Jun-Ichi Sasaki, Taka-Aki Asoh, Takuya Matsumoto, Hiroshi Egusa, Taiji Sohmura, Eben Alsberg, Mitsuru Akashi, Hirofumi Yatani

TISSUE ENGINEERING PART A 16 ( 8 ) 2497 - 2504 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.

DOI： 10.1089/ten.tea.2009.0523

Apatite Containing Aspartic Acid for Selective Protein Loading

M. Hafiz Uddin, T. Matsumoto, S. Ishihara, A. Nakahira, M. Okazaki, T. Sohmura

JOURNAL OF DENTAL RESEARCH 89 ( 5 ) 488 - 492 2010年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SAGE PUBLICATIONS INC

Physico-chemical modifications of hydroxyapatite (HAp) materials are considered as pre-requisites for the development of new bioactive carrier materials for drug delivery and tissue engineering applications. Since acidic amino acids have well-documented affinities to both HAp and basic proteins, HAp modified by aspartic acid (Asp, acidic amino acid) might be one of the candidate substrates for a basic protein carrier. Here, we synthesized HAp in the presence of various concentrations of Asp and observed that HAp crystallinity and other physico-chemical properties were effectively modulated. Detailed studies indicated that Asp was not incorporated in the HAp crystal lattice, but rather was trapped in HAp crystals. Protein adsorption studies indicated that the HAp particles modified by Asp had a selective loading capacity for basic protein. Therefore, HAp particles containing Asp might have potential in drug delivery applications, especially as the carrier of basic proteins including bFGF and BMP.

DOI： 10.1177/0022034509357309

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