論文 - 松本　卓也

合着用レジン配合型グラスアイオノマーセメントの硬化初期の接着性と曲げ特性

入江 正郎, 田仲 持郎, 松本 卓也, 武田 宏明, 鳥井 康弘, 丸尾 幸憲, 西川 悟郎, 長岡 紀幸

日本歯科理工学会誌   33 ( 2 )   153 - 153   2014年3月

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記述言語：日本語   出版者・発行元：(一社)日本歯科理工学会  

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miRNA-720 regulates the stem cell phenotype and differentiation of human dental pulp-derived mesenchymal stromal cells 査読

Hara, Emilio Satoshi, Ono, Mitsuaki, Eguchi, Takanori, Hai Thanh Pham, Tajima, Shoji, Calderwood, Stuart K., Matsumoto, Takuya, Kuboki, Takuo, IEEE

2014 International Symposium on Micro-Nanomechatronics and Human Science (Mhs)   1 - 2   2014年

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掲載種別：研究論文（学術雑誌）   出版者・発行元：IEEE  

DOI： 10.1109/MHS.2014.7006080

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1P176 原子間力顕微鏡によるマウス顎下腺組織の弾性率マッピング測定(12. 細胞生物的課題,ポスター,第52回日本生物物理学会年会(2014年度))

Nakamura Mitsuhiro, Fujii Yuki, Taketa Hiroaki, Matsumoto Takuya, Okajima Takaharu

生物物理   54 ( 1 )   S170   2014年

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記述言語：英語   出版者・発行元：一般社団法人 日本生物物理学会  

DOI： 10.2142/biophys.54.S170_2

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セルフアドヘッシヴ・レジンセメントのサーマルサイクル負荷後のジルコニアに対する接着強さと曲げ特性

入江 正郎, 田仲 持郎, 松本 卓也, 長岡 紀幸, 丸尾 幸憲, 西川 悟郎, 吉山 昌宏

接着歯学   31 ( 3 )   139 - 139   2013年11月

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記述言語：日本語   出版者・発行元：(一社)日本接着歯学会  

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最近のセルフアドヘッシヴ・レジンセメントの硬化初期における接着特性

丸尾 幸憲, 西川 悟郎, 入江 正郎, 長岡 紀幸, 松本 卓也, 皆木 省吾

接着歯学   31 ( 3 )   138 - 138   2013年11月

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記述言語：日本語   出版者・発行元：(一社)日本接着歯学会  

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ジルコニア表面に対するリン酸モノマー処理の検討

長岡 紀幸, 玉田 宜之, 入江 正郎, 吉原 久美子, 吉田 靖弘, 西川 悟郎, 丸尾 幸憲, 松本 卓也, 皆木 省吾, 早川 聡

日本歯科理工学会誌   32 ( 5 )   354 - 354   2013年9月

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記述言語：日本語   出版者・発行元：(一社)日本歯科理工学会  

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合着用レジン添加型グラスアイオノマーセメントの種々の被着体への接着性と曲げ特性 サーマルサイクルの影響

入江 正郎, 田仲 持郎, 松本 卓也, 丸尾 幸憲, 西川 悟郎, 吉山 昌宏

接着歯学   31 ( 2 )   45 - 52   2013年8月

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記述言語：日本語   出版者・発行元：(一社)日本接着歯学会  

6種の合着用レジン添加型グラスアイオノマーセメント[RMGICs:RelyX Luting Plus AutomixとVitremer Paste(両者3M ESPE)、Fuji Luting EXとFuji Luting S(両者GC)、SI-R21202とResiGlass Paste(両者松風)]の合着材としての有用性を検討する一環として、歯質、ジルコニア(Lava、3M ESPE)、金合金(GC's Casting Gold Type III M.C.、GC)や金銀パラジウム合金(CastWell M.C.12%Gold、GC)に対するせん断接着強さおよび曲げ特性(強さと弾性率)を硬化直後、1時間水中浸漬後、1日間水中浸漬後およびサーマルサイクル10,000回負荷(TC 10k)後に測定した。その結果、1時間水中浸漬後のRMGICの様々な接着強さは、硬化直後より有意に高く、1日間水中浸漬後の歯質接着強さは1時間水中浸漬後より有意に高かった。TC 10k後では多くのRMGICがそれぞれの測定項目で、大半が有意に低下した。曲げ特性に関しても前述と類似の傾向を示した。(著者抄録)

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Application of Cyclic Strain for Accelerated Skeletal Myogenic Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stromal Cells with Cell Alignment 査読

Hiroshi Egusa, Munemasa Kobayashi, Takuya Matsumoto, Jun-Ichi Sasaki, Shinya Uraguchi, Hirofumi Yatani

TISSUE ENGINEERING PART A   19 ( 5-6 )   770 - 782   2013年3月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MARY ANN LIEBERT, INC  

The fabrication of biomimetic skeletal myocyte constructs continues to present a challenge to functional tissue engineering. The skeletal myogenesis of bone marrow-derived mesenchymal stromal cells (BMSCs) to mimic the native tissue architecture offers great therapeutic promise, but remains particularly difficult. The aim of this study was to examine the possibility of accelerating the skeletal myogenic differentiation of BMSCs with an aligned structure by applying cyclic strain. Mouse BMSCs (mBMSCs) were plated on silicone sheets that were coated with fibronectin and subjected to cyclic 10% uniaxial strain when they reached 80%-90% cell confluency. Cells cultured in a growth medium that were subjected to cyclic strain at a frequency of 0.17 Hz (10 times/min) demonstrated a shift of alignment within 48 h from a completely random orientation to a well-aligned morphology with well-organized actin stress fibers that were parallel to the strain vector. The cyclic strain restricted the motility and proliferation of the aligned mBMSCs in the growth medium, which resulted in tight cellular contact in the cell population. When mBMSCs were subjected to cyclic strain in a myogenic medium, reverse transcription-polymerase chain reaction analysis demonstrated the upregulation of skeletal myogenic marker genes (myogenic factor 5 [Myf5], myogenin, and myogenic regulatory factor 4 [MRF4]), but not smooth muscle marker genes (myocardin and a-smooth muscle actin). In addition, immunocytochemistry showed that the mBMSCs fused to form multinucleated myosin-and myogenin-positive myotubes in the direction of the applied tension within 5 days. These results demonstrate that our simple method of applying of cyclic strain to cells cultured in a myogenic medium greatly accelerates the skeletal myogenic differentiation of mBMSCs with an aligned structure, and they highlight the importance of cellular alignment for creating physiologically relevant environments to study the myogenesis of BMSCs and engineer skeletal muscle.

DOI： 10.1089/ten.tea.2012.0164

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Application of Cyclic Strain for Accelerated Skeletal Myogenic Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stromal Cells with Cell Alignment 査読

Hiroshi Egusa, Munemasa Kobayashi, Takuya Matsumoto, Jun-Ichi Sasaki, Shinya Uraguchi, Hirofumi Yatani

TISSUE ENGINEERING PART A   19 ( 5-6 )   770 - 782   2013年3月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MARY ANN LIEBERT, INC  

The fabrication of biomimetic skeletal myocyte constructs continues to present a challenge to functional tissue engineering. The skeletal myogenesis of bone marrow-derived mesenchymal stromal cells (BMSCs) to mimic the native tissue architecture offers great therapeutic promise, but remains particularly difficult. The aim of this study was to examine the possibility of accelerating the skeletal myogenic differentiation of BMSCs with an aligned structure by applying cyclic strain. Mouse BMSCs (mBMSCs) were plated on silicone sheets that were coated with fibronectin and subjected to cyclic 10% uniaxial strain when they reached 80%-90% cell confluency. Cells cultured in a growth medium that were subjected to cyclic strain at a frequency of 0.17 Hz (10 times/min) demonstrated a shift of alignment within 48 h from a completely random orientation to a well-aligned morphology with well-organized actin stress fibers that were parallel to the strain vector. The cyclic strain restricted the motility and proliferation of the aligned mBMSCs in the growth medium, which resulted in tight cellular contact in the cell population. When mBMSCs were subjected to cyclic strain in a myogenic medium, reverse transcription-polymerase chain reaction analysis demonstrated the upregulation of skeletal myogenic marker genes (myogenic factor 5 [Myf5], myogenin, and myogenic regulatory factor 4 [MRF4]), but not smooth muscle marker genes (myocardin and a-smooth muscle actin). In addition, immunocytochemistry showed that the mBMSCs fused to form multinucleated myosin-and myogenin-positive myotubes in the direction of the applied tension within 5 days. These results demonstrate that our simple method of applying of cyclic strain to cells cultured in a myogenic medium greatly accelerates the skeletal myogenic differentiation of mBMSCs with an aligned structure, and they highlight the importance of cellular alignment for creating physiologically relevant environments to study the myogenesis of BMSCs and engineer skeletal muscle.

DOI： 10.1089/ten.tea.2012.0164

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PubMed

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ロカテック処理したジルコニア表面の断面TEM観察

玉田 宜之, 長岡 紀幸, 武田 宏明, 吉原 久美子, 入江 正郎, 西川 悟郎, 丸尾 幸憲, 吉田 靖弘, 鳥井 康弘, 松本 卓也, 皆木 省吾

日本歯科理工学会誌   32 ( 2 )   152 - 152   2013年3月

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記述言語：日本語   出版者・発行元：(一社)日本歯科理工学会  

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二ケイ酸リチウムガラスセラミックスの接着強さにシランカップリング剤分子中の加水分解基数が及ぼす影響

西川 悟郎, 入江 正郎, 丸尾 幸憲, 山本 美恵, 玉田 宜之, 前田 直人, 長岡 紀幸, 松本 卓也, 皆木 省吾

日本歯科理工学会誌   32 ( 2 )   154 - 154   2013年3月

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記述言語：日本語   出版者・発行元：(一社)日本歯科理工学会  

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Phosphorylated Pullulan Bioadhesive for Regeneration and Reconstruction of Bone and Tooth 査読

Yasuhiro Yoshida, Takumi Okihara, Mariko Nakamura, Takuya Matsumoto

BIOCERAMICS 24   529-530   516 - +   2013年

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記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）   出版者・発行元：TRANS TECH PUBLICATIONS LTD  

A biodegradable material that bonds to hard tissues such as bones and teeth is urgently needed for medical and dental applications. However, such materials are not available in today's clinical practice of orthopedics and dentistry. Therefore, we synthesized biodegradable phosphorylated pullulan to develop a biomaterial that combines primary properties such as high biocompatibility, good bonding potential to hard tissue, high strength, biodegradability, and osteoconductivity. The pharmacopoeial polysaccharide pullulan was chemically functionalized with dihydrogen phosphate groups. Phosphorylated pullulan was formed network by adding calcium ion, making the composite less soluble in water. Adhesive force measurement revealed that adhesiveness of the pastes before setting can be controlled through optimization of additives. In addition, histological evaluation revealed that phosphorylated pullulan-based composite possesses high biocompatibility. These results indicate that phosphorylated pullulan can be used as a key material for regeneration and reconstruction of bone and tooth.

DOI： 10.4028/www.scientific.net/KEM.529-530.516

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In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO2: HA formation, cellular affinity and organ culture

Sang-Hyun An, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroyuki Miyajima, Ramaswamy Narayanan, Satoshi Imazato, Kyo-Han Kim

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS   32 ( 8 )   2516 - 2522   2012年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Anodization is an easily viable technique useful for producing TiO2 coatings on titanium substrates. Nanocrystalline anodic TiO2 structure was produced on titanium at 20 V using 1 M Na2SO4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO2 was produced on titanium by applying a galvanostatic current density of 70 A/m(2) in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface. (C) 2012 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.msec.2012.07.034

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Porous zirconia/hydroxyapatite scaffolds for bone reconstruction 査読

Sang-Hyun An, Takuya Matsumoto, Hiroyuki Miyajima, Atsushi Nakahira, Kyo-Han Kim, Satoshi Imazato

DENTAL MATERIALS   28 ( 12 )   1221 - 1231   2012年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCI LTD  

Objective. Highly porous apatite-based bioceramic scaffolds have been widely investigated as three-dimensional (3D) templates for cell adhesion, proliferation, and differentiation promoting the bone regeneration. Their fragility, however, limits their clinical application especially for a large bone defect.
Methods. To address the hypothesis that using a ZrO2/hydroxyapatite (HAp) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO2/HAp composite scaffolds with different ZrO2/HAp ratios, and evaluated their characteristics. In addition, porous ZrO2/HAp scaffolds containing bone marrow derived stromal cells (BMSCs) were implanted into critical-size bone defects for 6 weeks in order to evaluate the bone tissue reconstruction with this material.
Results. The porosity of a ZrO2/HAp scaffold can be adjusted from 72% to 91%, and the compressive strength of the scaffold increased from 2.5 to 13.8 MPa when the ZrO2 content increased from 50 to 100 wt%. The cell adhesion and proliferation in the ZrO2/HAp scaffold was greatly improved when compared to the scaffold made with ZrO2 alone. Moreover, in vivo study showed that a BMSCs-loaded ZrO2/HAp scaffold provided a suitable 3D environment for BMSC survival and enhanced bone regeneration around the implanted material.
Significance. We thus showed that a porous ZrO2/HAp composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed in the treatment of large bone defects. (C) 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.dental.2012.09.001

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PubMed

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In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO2: HA formation, cellular affinity and organ culture 査読

Sang-Hyun An, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroyuki Miyajima, Ramaswamy Narayanan, Satoshi Imazato, Kyo-Han Kim

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS   32 ( 8 )   2516 - 2522   2012年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Anodization is an easily viable technique useful for producing TiO2 coatings on titanium substrates. Nanocrystalline anodic TiO2 structure was produced on titanium at 20 V using 1 M Na2SO4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO2 was produced on titanium by applying a galvanostatic current density of 70 A/m(2) in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface. (C) 2012 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.msec.2012.07.034

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合着用レジン添加型グラスアイオノマーセメントの歯質接着性と曲げ特性

入江 正郎, 田仲 持郎, 松本 卓也, 玉田 宜之, 丸尾 幸憲, 西川 悟郎, 吉山 昌宏

接着歯学   30 ( 4 )   137 - 144   2012年12月

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記述言語：日本語   出版者・発行元：(一社)日本接着歯学会  

5種の合着用レジン添加型グラスアイオノマーセメント[RMGICs:Fuji Luting EX、Fuji Luting S(ジーシー)、RelyX Luting Plus Automix、Vitremer Paste(スリーエム・エスペ)、ResiGlass Paste(松風)]の歯質接着性と曲げ特性を測定、レジンセメント[SA Luting(クラレノリタケ・デンタル)]と比較した。エナメル質と象牙質に対するせん断接着強さと曲げ強さと曲げ弾性率を、初期硬化、1時間水中浸漬後および1日間水中浸漬後測定した。1時間水中浸漬後のRMGICsとレジンセメントの歯質接着強さは、初期硬化より有意に高く、1日間水中浸漬後の歯質接着強さは1時間水中浸漬後より有意に高かった。曲げ特性に関しては類似の傾向を示したが、レジンセメントはRMGICsと比較して有意に高い値を示した。RMGICsのエナメル質と象牙質に対する接着強さと曲げ特性はそれぞれ有意な正の関係にあった。(著者抄録)

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HEMA Inhibits Interfacial Nano-layering of the Functional Monomer MDP

Y. Yoshida, K. Yoshihara, S. Hayakawa, N. Nagaoka, T. Okihara, T. Matsumoto, S. Minagi, A. Osaka, K. Van Landuyt, B. Van Meerbeek

JOURNAL OF DENTAL RESEARCH   91 ( 11 )   1060 - 1065   2012年11月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SAGE PUBLICATIONS INC  

Previous research showed that the functional monomer 10-methacryloxydecyl dihydrogen phosphate (MDP) ionically bonds to hydroxyapatite (HAp) and forms a nano-layered structure at the interface with HAp-based substrates. Such hydrophobic nano-layering is considered to contribute to the long-term durability of the bond to tooth tissue. However, dental adhesives are complex mixtures usually containing different monomers. This study investigated the effect of the monomer 2-hydroxyethylmethacrylate (HEMA) on the chemical interaction of MDP with HAp by x-ray diffraction (XRD), nuclear magnetic resonance (NMR), and quartz crystal microbalance (QCM). We examined the chemical interaction of 5 experimental MDP solutions with increasing concentrations of HEMA. XRD revealed that addition of HEMA inhibits nano-layering at the interface, while NMR confirmed that MDP remained adsorbed onto the HAp surface. QCM confirmed this adsorption of MDP to HAp, as well as revealed that the demineralization rate of HAp by MDP was reduced by HEMA. It was concluded that even though the adsorption of MDP to HAp was not hindered, addition of HEMA inhibited interfacial nano-layering. Potential consequences with regard to bond durability necessitate further research.

DOI： 10.1177/0022034512460396

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新しい多用途型処理材の接着強さからの検討

入江 正郎, 田仲 持郎, 松本 卓也, 玉田 宜之, 丸尾 幸憲, 西川 悟郎, 吉山 昌宏

接着歯学   30 ( 3 )   126 - 126   2012年11月

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記述言語：日本語   出版者・発行元：(一社)日本接着歯学会  

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汚染されたジルコニア表面に対するリン酸エステルによるカップリング処理

長岡 紀幸, 玉田 宜之, 入江 正郎, 西川 悟郎, 丸尾 幸憲, 吉田 靖弘, 松本 卓也

接着歯学   30 ( 3 )   114 - 114   2012年11月

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記述言語：日本語   出版者・発行元：(一社)日本接着歯学会  

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ジルコニアに対するリン酸モノマーの吸着特性

玉田 宜之, 長岡 紀幸, 早川 聡, 入江 正郎, 西川 悟郎, 丸尾 幸憲, 吉田 靖弘, 松本 卓也, 尾坂 明義, 皆木 省吾

日本歯科理工学会誌   31 ( 5 )   479 - 479   2012年9月

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記述言語：日本語   出版者・発行元：(一社)日本歯科理工学会  

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