研究者詳細 - 松本　卓也

論文 - 松本　卓也

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Effect of Biomechanical Environment on Degeneration of Meckel’s Cartilage 査読

M. Farahat, G.A.S. Kazi, E.S. Hara, T. Matsumoto

Journal of Dental Research 100 ( 2 ) 171 - 178 2021年2月

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：SAGE Publications

During orofacial tissue development, the anterior and posterior regions of the Meckel’s cartilage undergo mineralization, while the middle region undergoes degeneration. Despite the interesting and particular phenomena, the mechanisms that regulate the different fates of Meckel’s cartilage, including the effects of biomechanical cues, are still unclear. Therefore, the purpose of this study was to systematically investigate the course of Meckel’s cartilage during embryonic development from a biomechanical perspective. Histomorphological and biomechanical (stiffness) changes in the Meckel’s cartilage were analyzed from embryonic day 12 to postnatal day 0. The results revealed remarkable changes in the morphology and size of chondrocytes, as well as the occurrence of chondrocyte burst in the vicinity of the mineralization site, an often-seen phenomenon preceding endochondral ossification. To understand the effect of biomechanical cues on Meckel’s cartilage fate, a mechanically tuned 3-dimensional hydrogel culture system was used. At the anterior region, a moderately soft environment (10-kPa hydrogel) promoted chondrocyte burst and ossification. On the contrary, at the middle region, a more rigid environment (40-kPa hydrogel) enhanced cartilage degradation by inducing a higher expression of MMP-1 and MMP-13. These results indicate that differences in the biomechanical properties of the surrounding environment are essential factors that distinctly guide the mineralization and degradation of Meckel’s cartilage and would be valuable tools for modulating in vitro cartilage and bone tissue engineering.

DOI： 10.1177/0022034520960118

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その他リンク： http://journals.sagepub.com/doi/full-xml/10.1177/0022034520960118

Flexural Property of Composite Biomaterials Used in the Oral Cavity 査読

Masao Irie, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Preprints 2021 2021年2月

Nanostructural analysis of distinct nucleation sites in pathological mineralization 査読

Emilio Satoshi Hara, Susumu Oozawa, Noriyuki Nagaoka, Masahiro Okada, Shingo Kasahara, Takuya Matsumoto

Materials Advances 2 ( 13 ) 4423 - 4431 2021年

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：Royal Society of Chemistry (RSC)

<p>Vesicular and non-vesicular phospholipids were the nucleation sites in atherosclerotic calcification. Mineralization <italic>in vitro</italic> showed that LDL, PS and PC mineralized in 2 days. PS mineralized more than PC.</p>

DOI： 10.1039/d1ma00273b

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Large three-dimensional cell constructs for tissue engineering. 国際誌

Jun-Ichi Sasaki, Gabriela L Abe, Aonan Li, Takuya Matsumoto, Satoshi Imazato

Science and technology of advanced materials 22 ( 1 ) 571 - 582 2021年

記述言語：英語掲載種別：研究論文（学術雑誌）

Much research has been conducted on fabricating biomimetic biomaterials in vitro. Tissue engineering approaches are often conducted by combining cells, scaffolds, and growth factors. However, the degradation rate of scaffolds is difficult to control and the degradation byproducts occasionally limit tissue regeneration. To overcome these issues, we have developed a novel system using a thermo-responsive hydrogel that forms scaffold-free, three-dimensional (3D) cell constructs with arbitrary size and morphology. 3D cell constructs prepared using bone marrow-derived stromal stem cells (BMSCs) exhibited self-organizing ability and formed bone-like tissue with endochondral ossification. Endothelial cells were then introduced into the BMSC construct and a vessel-like structure was formed within the constructs. Additionally, the bone formation ability was promoted by endothelial cells and cell constructs could be freeze-dried to improve their clinical application. A pre-treatment with specific protein protectant allowed for the fabrication of novel bone substitutes composed only of cells. This 3D cell construct technology using thermo-responsive hydrogels was then applied to other cell species. Cell constructs composed of dental pulp stem cells were fabricated, and the resulting construct regenerated pulp-like tissue within a human pulpless tooth. In this review, we demonstrate the approaches for the in vitro fabrication of bone and dental pulp-like tissue using thermo-responsive hydrogels and their potential applications.

DOI： 10.1080/14686996.2021.1945899

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Flexural Strength of Resin Core Build-Up Materials: Correlation to Root Dentin Shear Bond Strength and Pull-Out Force. 査読国際誌

Masao Irie, Yukinori Maruo, Goro Nishigawa, Kumiko Yoshihara, Takuya Matsumoto

Polymers 12 ( 12 ) 2020年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

The aims of this study were to investigate the effects of root dentin shear bond strength and pull-out force of resin core build-up materials on flexural strength immediately after setting, after one-day water storage, and after 20,000 thermocycles. Eight core build-up and three luting materials were investigated, using 10 specimens (n = 10) per subgroup. At three time periods-immediately after setting, after one-day water storage, and after 20,000 thermocycles, shear bond strengths to root dentin and pull-out forces were measured. Flexural strengths were measured using a 3-point bending test. For all core build-up and luting materials, the mean data of flexural strength, shear bond strength and pull-out force were the lowest immediately after setting. After one-day storage, almost all the materials yielded their highest results. A weak, but statistically significant, correlation was found between flexural strength and shear bond strength (r = 0.508, p = 0.0026, n = 33). As the pull-out force increased, the flexural strength of core build-up materials also increased (r = 0.398, p = 0.0218, n = 33). Multiple linear regression analyses were conducted using these three independent factors of flexural strength, pull-out force and root dentin shear bond strength, which showed this relationship: Flexural strength = 3.264 × Shear bond strength + 1.533 × Pull out force + 10.870, p = 0.002). For all the 11 core build-up and luting materials investigated immediately after setting, after one-day storage and after 20,000 thermocycles, their shear bond strengths to root dentin and pull-out forces were correlated to the flexural strength in core build-up materials. It was concluded that the flexural strength results of the core build-up material be used in research and quality control for the predictor of the shear bond strength to the root dentin and the retentive force of the post.

DOI： 10.3390/polym12122947

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Micro-Architectural Investigation of Teleost Fish Rib Inducing Pliant Mechanical Property 査読

Yu Yang Jiao, Masahiro Okada, Emilio Satoshi Hara, Shi Chao Xie, Noriyuki Nagaoka, Takayoshi Nakano, Takuya Matsumoto

Materials 13 ( 22 ) 5099 - 5099 2020年11月

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Despite the fact that various reports have been discussing bone tissue regeneration, precise bone tissue manipulation, such as controlling the physical properties of the regenerated bone tissue, still remains a big challenge. Here, we focused on the teleost fish ribs showing flexible and tough mechanical properties to obtain a deeper insight into the structural and functional features of bone tissue from different species, which would be valuable for the superior design of bone-mimicking materials. Herein, we examined their compositions, microstructure, histology, and mechanical properties. The first rib of Carassius langsdorfii showed a higher Young’s modulus with a small region of chondrocyte clusters compared with other smaller ribs. In addition, highly oriented collagen fibers and osteocytes were observed in the first rib, indicating that the longest first rib would be more mature. Moreover, the layer-by-layer structure of the oriented bone collagen was observed in each rib. These microarchitectural and compositional findings of fish rib bone would give one the useful idea to reproduce such a highly flexible rib bone-like material.

DOI： 10.3390/ma13225099

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汚染除去材が接着性レジンセメントのせん断接着強さに及ぼす影響

矢部淳, 入江正郎, 岡田正弘, 野崎高儀, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 60 - 60 2020年11月

新規レジンセメントの歯質接着性からの検討各種処理材の影響

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 58 - 58 2020年11月

新規ボンディング材の歯質接着性からの検討湿潤vs.乾燥

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 59 - 59 2020年11月

新規セルフアドヒーシヴ・レジンセメントの接着性からの検討プライマー併用効果、Self-cure vs.Dual-cure

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

接着歯学 38 ( 3 ) 111 - 111 2020年9月

Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis 査読

Mst Nahid Akhter, Emilio Satoshi Hara, Koichi Kadoya, Masahiro Okada, Takuya Matsumoto

International Journal of Molecular Sciences 21 ( 15 ) 5327 - 5327 2020年7月

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Current stem cell-based techniques for bone-like tissue synthesis require at least two to three weeks. Therefore, novel techniques to promote rapid 3D bone-like tissue synthesis in vitro are still required. In this study, we explored the concept of using cell nanofragments as a substrate material to promote rapid bone formation in vitro. The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. The results showed that ultrasonication for 3 min allowed the fabrication of homogeneous nanofragments of less than 150 nm in length, which mineralized surprisingly in just one day, faster than the fragments obtained from all other methods. Further optimization of culture conditions indicated that a concentration of 10 mM or 100 mM of β-glycerophosphate enhanced, whereas fetal bovine serum (FBS) inhibited in a concentration-dependent manner, the mineralization of the cell nanofragments. Finally, a 3D collagen-cell nanofragment-mineral complex mimicking a bone-like structure was generated in just two days by combining the cell nanofragments in collagen gel. In conclusion, sonication for three min could be applied as a novel method to fabricate cell nanofragments of less than 150 nm in length, which can be used as a material for in vitro bone tissue engineering.

DOI： 10.3390/ijms21155327

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唾液汚染除去材処理がセラミックスの接着強さに与える影響

萬田陽介, 入江正郎, 丸尾幸憲, 西川悟郎, 吉原久美子, 長岡紀幸, 松本卓也, 皆木省吾

日本補綴歯科学会誌 12 ( 特別号 ) 171 - 171 2020年6月

ヒト血漿汚染被着面に対する汚染除去材の効果接着強さからの検討

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 152回 95 - 95 2020年6月

臼歯修復用手動練和型のグラスアイオノマーセメント I級窩洞適合性と歯質接着強さ

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 39 ( Special Issue75 ) 17 - 17 2020年4月

単斜晶ジルコニアとシリカを焼付した高透光性ジルコニアの機械的性質

岡田正弘, 宇野聡一郎, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 39 ( Special Issue75 ) 7 - 7 2020年4月

骨質指標としての骨基質配向化制御におけるオステオカルシンの役割

中野貴由, 小笹良輔, 岡田正弘, 松本卓也, 森石武史, 小守壽文

日本歯科理工学会誌 39 ( Special Issue75 ) 10 - 10 2020年4月

Toughening of highly translucent zirconia by monoclinic ZrO₂ and SiO₂ particle coating 査読

Soichiro UNO, Masahiro OKADA, Hiroaki TAKETA, Yasuhiro TORII, Takuya MATSUMOTO

Dental Materials Journal 39 ( 2 ) 295 - 301 2020年3月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Society for Dental Materials and Devices

The mechanical properties of highly translucent partially stabilized zirconia (PSZ) need to be improved; however, improvement of mechanical properties often decreases translucency. To overcome this problem, a monoclinic ZrO2 (mZrO2)/SiO2 dispersion was prepared and applied as a coating material for PSZ. The influence of surface treatment by the mZrO2/SiO2 dispersion on the surface topography, crystallography, and mechanical properties of highly translucent PSZ was investigated in this study. Following the treatment, the mechanical strength of highly translucent PSZ improved by 170% compared with control, for the best mZrO2/SiO2 dispersion ratio and heating temperature condition, while maintaining its translucency. The proposed coating is promising for improving the mechanical properties of highly translucent PSZ.

DOI： 10.4012/dmj.2018-415

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Titanium as an Instant Adhesive for Biological Soft Tissue 査読

Masahiro Okada, Emilio Satoshi Hara, Atsushi Yabe, Kei Okada, Yo Shibata, Yasuhiro Torii, Takayoshi Nakano, Takuya Matsumoto

ADVANCED MATERIALS INTERFACES 2020年3月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY

A variety of polymer- and ceramic-based soft-tissue adhesives have been developed as alternatives to surgical sutures, yet several disadvantages regarding the mechanical properties, biocompatibility, and handling hinder their further application particularly when applied for immobilization of implantable devices. Here, it is reported that a biocompatible and tough metal, titanium (Ti), shows instant and remarkable adhesion properties after acid treatment, demonstrated by ex vivo shear adhesion tests with mouse dermal tissues. Importantly, in vivo experiments demonstrate that the acid-treated Ti can easily and stably immobilize a device implanted in the mouse subcutaneous tissue. Collectively, the acid-treated Ti is shown as a solid-state instant adhesive material for biological soft tissues, which can have diverse applications including immobilization of body-implantable devices.

DOI： 10.1002/admi.201902089

Web of Science

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Vibratory splint therapy for decreasing sleep clenching: A pilot study 査読

Emilio Satoshi Hara, Andrea Lusvarghi Witzel, Hajime Minakuchi, Carlos Eduardo de Pitta, Rosane Tronquin Gallo, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Marcelo Costa Bolzan

CRANIO® 38 ( 1 ) 15 - 21 2020年1月

Flexural properties, bond ability, and crystallographic phase of highly translucent multi-layered zirconia

Yukinori Maruo, Kumiko Yoshihara, Masao Irie, Goro Nishigawa, Noriyuki Nagaoka, Takuya Matsumoto, Shogo Minagi

Journal of Applied Biomaterials and Functional Materials 18 2020年

掲載種別：研究論文（学術雑誌）

This study investigated the mechanical properties, bond ability, and crystallographic forms of different sites in a highly translucent, multi-layered zirconia disk. Flexural properties, bond ability to resin cement, and phase composition were investigated at three sites of a highly translucent, multi-layered zirconia disk: incisal, middle, and cervical. Flexural strength (FS) and flexural modulus (FM) were measured with static three-point flexural test. Shear bond strength (SB) to resin cement was measured after 24 h storage (37°C). Phase composition under mechanical stress was analyzed using X-ray diffraction. Without air abrasion, FS at the incisal site yielded the lowest value and was significantly lower than the middle and cervical sites. Air abrasion lowered the FS of each site. FM at the incisal site without air abrasion showed the significantly lowest value, and air abrasion increased its FM value. At the middle and cervical sites, their FM values were higher than the incisal site but were not significantly affected by air abrasion. SB value did not show significant differences among the sites. After sintering, cubic zirconia was detected at each site. Rhombohedral phase transformation occurred after mirror polishing. In highly translucent, multi-layered zirconia which was mainly composed of cubic zirconia, rhombohedral phase transformation occurred under mechanical stress and resulted in weakened mechanical properties.

DOI： 10.1177/2280800020942717

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論文 - 松本 卓也

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論文 - 松本　卓也