論文 - 松本　卓也

Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo 査読

Y Hamada, K Yuki, M Okazaki, W Fujitani, T Matsumoto, MK Hashida, K Harutsugu, K Nokihara, M Daito, N Matsuura, J Takahashi

DENTAL MATERIALS JOURNAL   23 ( 4 )   650 - 655   2004年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES  

Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.

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Angiogenic activity of osteopontin-derived peptide SVVYGLR 査読

Y Hamada, K Nokihara, M Okazaki, W Fujitani, T Matsumoto, M Matsuo, Y Umakoshi, J Takahashi, N Matsuura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   310 ( 1 )   153 - 157   2003年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE  

Angiogenesis plays an important role in various pathological conditions as well as some physiological processes. Although a number of soluble angiogenic factors have been reported, extracellular matrix also has crucial effect on angiogenesis through interaction with endothelial cells. Since recent reports showed osteopontin had some angiogenic activity, the effect of the SVVYGLR peptide, novel binding motif in osteopontin molecule, on angiogenesis was examined in this study. Synthetic peptide SVVYGLR did not have proliferative effect on endothelial cells but adhesion and migration activity to endothelial cells. Furthermore, SVVYGLR had as potent activity for tube formation in three-dimensional collagen gel as vascular endothelial growth factor which is known to be the strongest angiogenic factor. Electron microscopical analysis showed a number of microvilli on the endothelial luminar surface and tight junction formation in the luminar intercellular border between endothelial cells, indicating SVVYGLR induced cell porarity and differentiation of endothelial cells. This small peptide might be expected to stimulate angiogenesis to improve some ischemic conditions in the future because of some advantages due to smaller molecular weight. (C) 2003 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2003.09.001

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Control of pore size in L-lactide/epsilon-caprolactone copolymer foams for tissue regeneration by the freeze-drying method 査読

H Nakao, SH Hyon, S Tsutsumi, T Matsumoto, J Takahashi

DENTAL MATERIALS JOURNAL   22 ( 3 )   262 - 271   2003年9月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES  

In the regeneration,and repair of missing tissues, synthetic polymer scaffolds need many pores to involve cells and to supply cells with nutrients. The control of the pore size of biodegradable L-lactide/epsilon-caprolactone copolymer foams was studied by changing the polymer concentration and the cooling temperature in the freeze-drying method. The mixtures of polymer and 1, 4-dioxane solution were poured into an 18-8 stainless steel Petri dish and frozen. The pore size of a polymer foam tends to increase from the bottom towards the top of a Petri dish. The pore size decreased to one-half with increasing polymer concentration (1 to 10 wt%). The mean pore size in foams of 8% polymer concentration decreased from 100mum to 20mum as cooling temperature was lowered. This suggests that the,higher cooling rate due to lower cooling temperature can produce smaller ice-crystals and result in smaller pores.

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Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts

M Taira, H Nakao, T Matsumoto, J Takahashi

INTERNATIONAL JOURNAL OF PROSTHODONTICS   13 ( 4 )   311 - 315   2000年7月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：QUINTESSENCE PUBL CO INC  

Purpose: The objective of this study was to examine the cytotoxic effect of methyl methacrylate (MMA) monomer on 4 mammalian fibroblasts. Materials and Methods: Four cells-C3H10T1/2, L929, Balb/3T3 clone A31, and MC3T3-E1-were incubated for 6 days in 24-well microplates filled with 1 mL of Dulbecco's Modified Eagle medium containing MMA monomer that ranged from 0 to 200 mM/L. Cytotoxicity was evaluated by direct cell number count. Results: L929 possessed the largest TC,, value (the amount of MMA that caused 50% cell death) (34 mM/L), and Balb/3T3 clone A31 had the least value (1 mM/L); C3H10T1/2 and MC3T3-E1 had intermediate values (25 mM/L and 16 mM/L, respectively). Conclusion: This study showed that the MMA monomer had dose-dependent cytotoxicity, which Varied among the cells tested.

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Inhibiting action of carbohydrates on the growth of fluorapatite crystals

T Matsumoto, M Okazaki, M Taira, J Takahashi

CARIES RESEARCH   34 ( 1 )   26 - 32   2000年1月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：KARGER  

Hydroxyapatite (HAp) and fluorapatite (FAp) were synthesized in the presence of carbohydrates (glucose, fructose, galactose and sucrose), X-ray diffraction analysis showed that the crystallinity of two FAp groups that were synthesized in the presence of glucose or sucrose decreased with the increase in carbohydrate content, In SEM photographs we found that the size of their crystals was small and the shape changed. However, FAp groups that were snythesized in the presence of fructose or galactose showed little change in their crystallinity and shape, Four carbohydrates had some influence on size, but not on shape or crystallinity when synthesizing HAp. These results suggest that glucose and/or sucrose has a chemical affinity to FAp and inhibits the growth of FAp crystals, Copyright (C) 2000 S. Karger AG, Basel.

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Organ-derived dependence of biomineralization in type I collagen gel 査読

T Matsumoto, M Okazaki, M Inoue, Y Hamada, M Taira, J Takahashi

BIOCERAMICS   192-1   709 - 712   2000年

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：TRANS TECH PUBLICATIONS LTD  

The number and the structure of cross-links in type I collagen are different from each organ, therefore, orientation and diameter of type I collagen fibers are also different from each organ. When type I collagen is used as biomaterials for hard tissue regeneration, it can be considered that the characteristics and the quantity of newly created minerals in type I collagen are different depending on organs-derived. In this study, calcium phosphate were synthesized in type I collagen gels derived from pig skin or tendon and bovine bone, and the characteristics of obtained minerals were investigated. It was suggested that all the newly created products were octacalcium phosphate (OCP) in X-ray diffraction analysis. Furthermore, X-ray diffraction analysis showed that minerals obtained in bone collagen gel were significantly high in crystallinity compared with minerals obtained in skin and tendon collagen gel. However, the quantity of the newly created minerals in bone collagen decreased significantly compared with minerals obtained in skin and tendon collagen. Collectively these results suggest that the characteristics and quantity of minerals synthesized in type I collagen are depending on organ of origin.

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Functionally graded fluoridated apatites

M Okazaki, Y Miake, H Tohda, T Yanagisawa, T Matsumoto, J Takahashi

BIOMATERIALS   20 ( 15 )   1421 - 1426   1999年8月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCI LTD  

Fluoridated hydroxyapatite was synthesized at 80 +/- 1 degrees C and pH 7.4 +/- 0.2 using a gradient fluoride supply system. X-ray diffraction analysis showed a typically apatitic pattern, although the (3 0 0) reflection was broader than that of homogeneous fluorapatite. Scanning electron micrographic observation indicated that the apatite was composed of rod-like crystals similarly to fluorapatite. High-resolution transmission electron microscopy showed electron damage in the core of the crystal. When the apatite pellet was prepared, electron spectroscopy for chemical analysis showed a negative gradient of fluoride concentration with depth in the crystals. The apparent solubility in 0.5 mol/l acetate buffer solution (37 degrees C and pH 4.0) was 9.16 +/- 0.39 mmol/l, much less than that of homogeneous hydroxyapatite 32.3 +/- 1.9 mmol/l, and less than that of heterogeneous two-layer fluoridated apatite with an outer fluoride-rich layer 12.5 +/- 0.6 mmol/l, which was synthesized previously by supplying fluoride during the latter half of the experimental period. These results suggest that graded fluoridated apatite may be formed by this process and have higher acid resistance than two-layer fluoridated apatite. (C) 1999 Elsevier Science Ltd. All rights reserved.

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