研究者詳細 - 松本　卓也

論文 - 松本　卓也

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Co-precipitating calcium phosphate as oral detoxification of cadmium 査読

Ahmad Bikharudin, Masahiro Okada, Ping-chin Sung, Takuya Matsumoto

Journal of Hazardous Materials 487 137307 - 137307 2025年4月

Optimizing β-TCP with E-rhBMP-2-infused fibrin for vertical bone regeneration in a mouse calvarium model

Kun Zhao, Mitsuaki Ono, Xindi Mu, Ziyi Wang, Shichao Xie, Tomoko Yonezawa, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Toshitaka Oohashi

Regenerative Biomaterials 12 2025年1月

掲載種別：研究論文（学術雑誌）出版者・発行元：Oxford University Press (OUP)

Abstract

Effective reconstruction of large bone defects, particularly in thickness, remains one of the major challenges in orthopedic and dental fields. We previously produced an Escherichia coli-based industrial-scale GMP-grade recombinant human bone morphogenetic protein-2 (E-rhBMP-2) and showed that the combination of E-rhBMP-2 with beta-tricalcium phosphate (β-TCP/E-rhBMP-2) can effectively promote bone reconstruction. However, the limited mechanical strength and poor morphology retention of β-TCP granules are key points that need optimization to obtain more effective grafts and further expand its clinical applications. Therefore, we combined β-TCP/E-rhBMP-2 with fibrin gel to enhance its mechanical properties and usability for vertical bone regeneration. We investigated the mechanical properties and vertical bone regeneration effects of the materials applied, with or without fibrin containing E-rhBMP-2, in a calvarial defect model in mice. Compression tests were conducted to assess the initial stability of the materials. Scanning electron microscopy and Fourier transform infrared spectroscopy were conducted to characterize the presence of fibrin on the scaffold. After 4 and 12 weeks of implantation, micro-computed tomography and histological and immunofluorescent analyses were performed to assess the morphology and volume of the newly formed bone. The fibrin-containing groups had significantly higher initial mechanical strength and higher ability to maintain their morphology in vivo compared to the counterparts without fibrin. However, fibrin gel alone suppressed the bone formation ability of β-TCP/E-rhBMP-2 whereas the presence of high doses of E-rhBMP-2 in fibrin gel resulted in material resorption and enhanced new bone formation. In conclusion, fibrin gel significantly improved the mechanical strength and surgical manageability of the β-TCP/E-rhBMP-2 scaffold, and the addition of E-rhBMP-2 to the fibrin gel further enhanced the vertical bone regeneration and initial structural integrity of the scaffold.

DOI： 10.1093/rb/rbae144

その他リンク： https://academic.oup.com/rb/article-pdf/doi/10.1093/rb/rbae144/61841803/rbae144.pdf

Long-Term Bonding Performance of One-Bottle vs. Two-Bottle Bonding Agents to Lithium Disilicate Ceramics 査読

Masao Irie, Masahiro Okada, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Polymers 16 ( 16 ) 2266 - 2266 2024年8月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

The aim of this study was to compare the long-term bonding performance to lithium disilicate (LDS) ceramic between one-bottle and two-bottle bonding agents. Bonding performance was investigated under these LDS pretreatment conditions: with hydrofluoric acid (HF) only, without HF, with a two-bottle bonding agent (Tokuyama Universal Bond II) only. Shear bond strengths between LDS and nine resin cements (both self-adhesive and conventional adhesive types) were measured at three time periods: after one-day water storage (Base), and after 5000 and 20,000 thermocycles (TC 5k and TC 20k respectively). Difference in degradation between one- and two-bottle bonding agents containing the silane coupling agent was compared by high-performance liquid chromatography. With HF pretreatment, bond strengths were not significantly different among the three time periods for each resin cement. Without HF, ESTECEM II and Super-Bond Universal showed significantly higher values than others at TC 5k and TC 20k when treated with the recommended bonding agents, especially at TC 20k. Difference in degradation between one- and two-bottle bonding agents containing the silane coupling agent was compared by high-performance liquid chromatography (HPLC). For both cements, these values at TC 20k were also not significantly different from pretreatment with only Tokuyama Universal Bond II. For LDS, long-term bond durability could be maintained by pretreatment with Tokuyama Universal Bond II instead of the hazardous HF.

DOI： 10.3390/polym16162266

The cell membrane as biofunctional material for accelerated bone repair. 査読国際誌

Emi Hatano, Nahid Akhter, Risa Anada, Mitsuaki Ono, Toshitaka Oohashi, Takuo Kuboki, Hiroshi Kamioka, Masahiro Okada, Takuya Matsumoto, Emilio Satoshi Hara

Acta biomaterialia 2024年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

The cell (plasma) membrane is enriched with numerous receptors, ligands, enzymes, and phospholipids that play important roles in cell-cell and cell-extracellular matrix interactions governing, for instance, tissue development and repair. We previously showed that plasma membrane nanofragments (PMNFs) act as nucleation sites for bone formation in vivo, and induce in vitro mineralization within 1 day. In this study, we optimized the methods for generating, isolating, and applying PMNFs as a cell-free therapeutic to expedite bone defect repair. The PMNFs were isolated from different mouse cell lines (chondrocytes, osteoblasts, and fibroblasts), pre-conditioned, lyophilized, and subsequently transplanted into 2 mm critical-sized calvarial defects in mice (n = 75). The PMNFs from chondrocytes, following a 3-day pre-incubation, significantly accelerated bone repair within 2 weeks, through a coordinated attraction of macrophages, endothelial cells, and osteoblasts to the healing site. In vitro experiments confirmed that PMNFs enhanced cell adhesion. Comparison of the PMNF efficacy with phosphatidylserine, amorphous calcium phosphate (ACP), and living cells confirmed the unique ability of PMNFs to promote accelerated bone repair. Importantly, PMNFs promoted nearly complete integration of the regenerated bone with native tissue after 6 weeks (% non-integrated bone area = 15.02), contrasting with the partial integration (% non-integrated bone area = 56.10; p < 0.01, Student's test) with transplantation of ACP. Vickers microhardness tests demonstrated that the regenerated bone after 6 weeks (30.10 ± 1.75) exhibited hardness similar to native bone (31.07 ± 2.46). In conclusion, this is the first study to demonstrate that cell membrane can be a promising cell-free material with multifaceted biofunctional properties that promote accelerated bone repair. STATEMENT OF SIGNIFICANCE.

DOI： 10.1016/j.actbio.2024.07.037

Water-Mediated On-Demand Detachable Solid-State Adhesive of Porous Hydroxyapatite for Internal Organ Retractions. 査読国際誌

Masahiro Okada, Shi Chao Xie, Yusuke Kobayashi, Hiroaki Yanagimoto, Daisuke Tsugawa, Masaru Tanaka, Takayoshi Nakano, Takumi Fukumoto, Takuya Matsumoto

Advanced healthcare materials e2304616 2024年5月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Novel adhesives for biological tissues offer an advanced surgical approach. Here, the authors report the development and application of solid-state adhesives consisting of porous hydroxyapatite (HAp) biocompatible ceramics as novel internal organ retractors. The operational principles of the porous solid-state adhesives are experimentally established in terms of water migration from biological soft tissues into the pores of the adhesives, and their performance is evaluated on several soft tissues with different hydration states. As an example of practical medical utility, HAp adhesive devices demonstrate the holding ability of porcine livers and on-demand detachability in vivo, showing great potential as internal organ retractors in laparoscopic surgery.

DOI： 10.1002/adhm.202304616

Lipids and Minerals, Interplay in Biomineralization: Nature's Alchemy 査読

Bhingaradiya Nutan, Masahiro Okada, Takuya Matsumoto

Tissue Engineering Part B: Reviews 2024年4月

Adaptive enhancement of apatite crystal orientation and Young's modulus under elevated load in rat ulnar cortical bone 査読

Jun Wang, Takuya Ishimoto, Tadaaki Matsuzaka, Aira Matsugaki, Ryosuke Ozasa, Takuya Matsumoto, Mikako Hayashi, Hyoung Seop Kim, Takayoshi Nakano

Bone 181 2024年4月

掲載種別：研究論文（学術雑誌）

Functional adaptation refers to the active modification of bone structure according to the mechanical loads applied daily to maintain its mechanical integrity and adapt to the environment. Functional adaptation relates to bone mass, bone mineral density (BMD), and bone morphology (e.g., trabecular bone architecture). In this study, we discovered for the first time that another form of bone functional adaptation of a cortical bone involves a change in bone quality determined by the preferential orientation of apatite nano-crystallite, a key component of the bone. An in vivo rat ulnar axial loading model was adopted, to which a 3–15 N compressive load was applied, resulting in approximately 440–3200 μɛ of compression in the bone surface. In the loaded ulnae, the degree of preferential apatite c-axis orientation along the ulnar long axis increased in a dose-dependent manner up to 13 N, whereas the increase in BMD was not dose-dependent. The Young's modulus along the same direction was enhanced as a function of the degree of apatite orientation. This finding indicates that bone has a mechanism that modifies the directionality (anisotropy) of its microstructure, strengthening itself specifically in the loaded direction. BMD, a scalar quantity, does not allow for load-direction-specific strengthening. Functional adaptation through changes in apatite orientation is an excellent strategy for bones to efficiently change their strength in response to external loading, which is mostly anisotropic.

DOI： 10.1016/j.bone.2024.117024

Mid-infrared passive spectroscopic imaging for visualizing tooth quality 査読

So Yamashita, Masahiro Okada, Takuya Matsumoto, Ichiro Ishimaru

Journal of Materials Chemistry B 2024年

Solid-state inorganic and metallic adhesives for soft biological tissues 査読

Masahiro Okada, Takuya Matsumoto

Japanese Dental Science Review 59 439 - 445 2023年12月

Fabrication of initial trabecular bone inspired three-dimensional structure with cell membrane nanofragments 査読

Koichi Kadoya, Emilio Satoshi Hara, Masahiro Okada, Yu Yang Jiao, Takayoshi Nakano, Akira Sasaki, Takuya Matsumoto

Regenerative Biomaterials 10 rbac088 2023年12月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Oxford University Press (OUP)

Abstract

The extracellular matrix of trabecular bone has a large surface exposed to the bone marrow and plays important roles such as hematopoietic stem cell niche formation and maintenance. In vitro reproduction of trabecular bone microenvironment would be valuable not only for developing a functional scaffold for bone marrow tissue engineering but also for understanding its biological functions. Herein, we analyzed and reproduced the initial stages of trabecular bone formation in mouse femur epiphysis. We identified that the trabecular bone formation progressed through the following steps: (1) partial rupture of hypertrophic chondrocytes; (2) calcospherite formation on cell nanofragments (CNFs) derived from the ruptured cell membranes; and (3) calcospherite growth and fusion to form the initial three-dimensional (3D) structure of trabecular bones. For reproducing the initial trabecular bone formation in vitro, we collected CNFs from cultured cells and used as nucleation sites for biomimetic calcospherite formation. Strikingly, almost the same 3D structure of the initial trabecular bone could be obtained in vitro by using additional CNFs as a binder to fuse biomimetic calcospherites.

DOI： 10.1093/rb/rbac088

Investigation on bacterial capture and antibacterial properties of acid-treated Ti surface 査読

Haruyuki Aoyagi, Masahiro Okada, Hiroaki Yanagimoto, Takuya Matsumoto

Dental Materials 2023年12月

Fabrication of a Fish-Bone-Inspired Inorganic–Organic Composite Membrane 査読

YuYang Jiao, Masahiro Okada, Bhingaradiya Nutan, Noriyuki Nagaoka, Ahmad Bikharudin, Randa Musa, Takuya Matsumoto

Polymers 15 ( 20 ) 4190 - 4190 2023年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Biological materials have properties like great strength and flexibility that are not present in synthetic materials. Using the ribs of crucian carp as a reference, we investigated the mechanisms behind the high mechanical properties of this rib bone, and found highly oriented layers of calcium phosphate (CaP) and collagen fibers. To fabricate a fish-rib-bone-mimicking membrane with similar structure and mechanical properties, this study involves (1) the rapid synthesis of plate-like CaP crystals, (2) the layering of CaP–gelatin hydrogels by gradual drying, and (3) controlling the shape of composite membranes using porous gypsum molds. Finally, as a result of optimizing the compositional ratio of CaP filler and gelatin hydrogel, a CaP filler content of 40% provided the optimal mechanical properties of toughness and stiffness similar to fish bone. Due to the rigidity, flexibility, and ease of shape control of the composite membrane materials, this membrane could be applied as a guided bone regeneration (GBR) membrane.

DOI： 10.3390/polym15204190

歯根膜応用を目指した石灰化dECM膜のin vivo骨結合性評価

鈴木美加, 木村剛, 岡田正弘, 松本卓也, 山田将博, 江草宏, 中村奈緒子, 岸田晶夫

日本歯科理工学会誌 42 ( Special Issue81 ) 18 - 18 2023年3月

Preparation of mineralized pericardium by alternative soaking for soft–hard interregional tissue application 査読

Mika Suzuki, Tsuyoshi Kimura, Yuta Nakano, Mako Kobayashi, Masahiro Okada, Takuya Matsumoto, Naoko Nakamura, Yoshihide Hashimoto, Akio Kishida

Journal of Biomedical Materials Research Part A 111 ( 2 ) 198 - 208 2023年2月

Shear Bond Strength of Resin Luting Materials to Lithium Disilicate Ceramic: Correlation between Flexural Strength and Modulus of Elasticity 査読

Masao Irie, Masahiro Okada, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Polymers 15 ( 5 ) 1128 - 1128 2023年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

This study investigates the effect of the curing mode (dual-cure vs. self-cure) of resin cements (four self-adhesive and seven conventional cements) on their flexural strength and flexural modulus of elasticity, alongside their shear bond strength to lithium disilicate ceramics (LDS). The study aims to determine the relationship between the bond strength and LDS, and the flexural strength and flexural modulus of elasticity of resin cements. Twelve conventional or adhesive and self-adhesive resin cements were tested. The manufacturer’s recommended pretreating agents were used where indicated. The shear bond strengths to LDS and the flexural strength and flexural modulus of elasticity of the cement were measured immediately after setting, after one day of storage in distilled water at 37 °C, and after 20,000 thermocycles (TC 20k). The relationship between the bond strength to LDS, flexural strength, and flexural modulus of elasticity of resin cements was investigated using a multiple linear regression analysis. For all resin cements, the shear bond strength, flexural strength, and flexural modulus of elasticity were lowest immediately after setting. A clear and significant difference between dual-curing and self-curing modes was observed in all resin cements immediately after setting, except for ResiCem EX. Regardless of the difference of the core-mode condition of all resin cements, flexural strengths were correlated with the LDS surface upon shear bond strengths (R2 = 0.24, n = 69, p < 0.001) and the flexural modulus of elasticity was correlated with them (R2 = 0.14, n = 69, p < 0.001). Multiple linear regression analyses revealed that the shear bond strength was 17.877 + 0.166, the flexural strength was 0.643, and the flexural modulus was (R2 = 0.51, n = 69, p < 0.001). The flexural strength or flexural modulus of elasticity may be used to predict the bond strength of resin cements to LDS.

DOI： 10.3390/polym15051128

Important roles of odontoblast membrane phospholipids in early dentin mineralization 査読国際誌

Risa Anada, Emilio Satoshi Hara, Noriyuki Nagaoka, Masahiro Okada, Hiroshi Kamioka, Takuya Matsumoto

Journal of Materials Chemistry B 11 ( 3 ) 657 - 666 2023年1月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Royal Society of Chemistry (RSC)

The objective of this study was to first identify the timing and location of early mineralization of mouse first molar, and subsequently, to characterize the nucleation site for mineral formation...

DOI： 10.1039/d2tb02351b

Host bone microstructure for enhanced resistance to bacterial infections 査読

Ryota Watanabe, Aira Matsugaki, Ozkan Gokcekaya, Ryosuke Ozasa, Takuya Matsumoto, Hiroyuki Takahashi, Hidekazu Yasui, Takayoshi Nakano

Biomaterials Advances 2023年

掲載種別：研究論文（学術雑誌）

Postoperative bacterial infection is a serious complication of orthopedic surgery. Not only infections that develop in the first few weeks after surgery but also late infections that develop years after surgery are serious problems. However, the relationship between host bone and infection activation has not yet been explored. Here, we report a novel association between host bone collagen/apatite microstructure and bacterial infection. The bone-mimetic-oriented micro-organized matrix structure was obtained by prolonged controlled cell alignment using a grooved-structured biomedical titanium alloy. Surprisingly, we have discovered that highly aligned osteoblasts have a potent inhibitory effect on Escherichia coli adhesion. Additionally, the oriented collagen/apatite micro-organization of the bone matrix showed excellent antibacterial resistance against Escherichia coli. The proposed mechanism for realizing the antimicrobial activity of the micro-organized bone matrix is by the controlled secretion of the antimicrobial peptides, including β-defensin 2 and β-defensin 3, from the highly aligned osteoblasts. Our findings contribute to the development of anti-infective strategies for orthopedic surgeries. The recovery of the intrinsically ordered bone matrix organization provides superior antibacterial resistance after surgery.

DOI： 10.1016/j.bioadv.2023.213633

Self-adhesive Devices Made of Titanium for Biological Soft Tissue 査読

Masahiro Okada, Takuya Matsumoto

Materia Japan 61 ( 11 ) 760 - 764 2022年11月

Does Multifunctional Acrylate’s Addition to Methacrylate Improve Its Flexural Properties and Bond Ability to CAD/CAM PMMA Block? 査読国際誌

Yukinori Maruo, Kumiko Yoshihara, Masao Irie, Noriyuki Nagaoka, Takuya Matsumoto, Shogo Minagi

Materials 15 ( 21 ) 7564 - 7564 2022年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

This study investigated the effects of a multifunctional acrylate copolymer—Trimethylolpropane Triacrylate (TMPTA) and Di-pentaerythritol Polyacrylate (A-DPH)—on the mechanical properties of chemically polymerized acrylic resin and its bond strength to a CAD/CAM polymethyl methacrylate (PMMA) disk. The methyl methacrylate (MMA) samples were doped with one of the following comonomers: TMPTA, A-DPH, or Trimethylolpropane Trimethacrylate (TMPTMA). The doping ratio ranged from 10 wt% to 50 wt% in 10 wt% increments. The flexural strength (FS) and modulus (FM) of PMMA with and without comonomer doping, as well as the shear bond strength (SBS) between the comonomer-doped PMMA and CAD/CAM PMMA disk, were evaluated. The highest FS (93.2 ± 4.2 MPa) was obtained when doped with 20 wt% of TMPTA. For TMPTMA, the FS decreased with the increase in the doping ratio. For SBS, TMPTA showed almost constant values (ranging from 7.0 to 8.2 MPa) regardless of the doping amount, and A-DPH peaked at 10 wt% doping (8.7 ± 2.2 MPa). TMPTMA showed two peaks at 10 wt% (7.2 ± 2.6 MPa) and 40 wt% (6.5 ± 2.3 MPa). Regarding the failure mode, TMPTMA showed mostly adhesive failure between the CAD/CAM PMMA disk and acrylic resin while TMPTA and A-DPH showed an increased rate of cohesive or mixed failures. Acrylate’s addition as a comonomer to PMMA provided improved mechanical properties and bond strength to the CAD/CAM PMMA disk.

DOI： 10.3390/ma15217564

Distinct Morphologies of Bone Apatite Clusters in Endochondral and Intramembranous Ossification. 査読国際誌

Emilio Satoshi Hara, Noriyuki Nagaoka, Masahiro Okada, Takayoshi Nakano, Takuya Matsumoto

Advanced biology 6 ( 11 ) e2200076 2022年7月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Bone apatite crystals grow in clusters, but the microstructure of these clusters is unknown. This study compares the structural and compositional differences between bone apatite clusters formed in intramembranous (IO) and endochondral ossification (EO). Calvaria (IO) and femurs (EO) are isolated from mice at embryonic days (E) 14.5 to 15.5 and post-natal days (P) 6 to 7, respectively. Results show that the initially formed bone apatite clusters in EO (≅1.2 µm2 ) are >10 times larger than those in IO (≅0.1 µm2 ), without significant changes in ion composition. In IO (E14.5 calvarium), early minerals are formed inside matrix vesicles (MVs). In contrast, in EO (P6 femur epiphysis), no MVs are observed, and chondrocyte-derived plasma membrane nanofragments (PMNFs) are the nucleation site for mineralization. Apatite cluster size difference is linked with the different nucleation sites. Moreover, an alkaline pH and slow P supply into a Ca-rich microenvironment are suggested to facilitate apatite cluster growth, as demonstrated in a biomimetic mineralization system. Together, the results reveal for the first time the distinct and exquisite microstructures of bone apatite clusters in IO and EO, and provide insightful inspirations for the design of more efficient materials for bone tissue engineering and repair.

DOI： 10.1002/adbi.202200076

A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading 査読

Ryota Watanabe, Aira Matsugaki, Takuya Ishimoto, Ryosuke Ozasa, Takuya Matsumoto, Takayoshi Nakano

International Journal of Molecular Sciences 23 ( 13 ) 7423 - 7423 2022年7月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

The anisotropic microstructure of bone, composed of collagen fibers and biological apatite crystallites, is an important determinant of its mechanical properties. Recent studies have revealed that the preferential orientation of collagen/apatite composites is closely related to the direction and magnitude of in vivo principal stress. However, the mechanism of alteration in the collagen/apatite microstructure to adapt to the mechanical environment remains unclear. In this study, we established a novel ex vivo bone culture system using embryonic mouse femurs, which enabled artificial control of the mechanical environment. The mineralized femur length significantly increased following cultivation; uniaxial mechanical loading promoted chondrocyte hypertrophy in the growth plates of embryonic mouse femurs. Compressive mechanical loading using the ex vivo bone culture system induced a higher anisotropic microstructure than that observed in the unloaded femur. Osteocytes in the anisotropic bone microstructure were elongated and aligned along the long axis of the femur, which corresponded to the principal loading direction. The ex vivo uniaxial mechanical loading successfully induced the formation of an oriented collagen/apatite microstructure via osteocyte mechano-sensation in a manner quite similar to the in vivo environment.

DOI： 10.3390/ijms23137423

In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel 査読

Jae Won Lee, Hyun Seung Kim, Soo-Jeong Yon, Takuya Matsumoto, Sang-Kyung Lee, Kuen Yong Lee

International Journal of Biological Macromolecules 209 1893 - 1899 2022年6月

Shear bond strength of resin cement on moist dentin and its relation to the flexural strength of resin cement. 査読

Masao Irie, Masahiro Okada, Akio Yoshimoto, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Dental materials journal 41 ( 3 ) 429 - 439 2022年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

We sought to compare the bond strength of resin cement on moist dentin to that on dry dentin, and determine the relationship between the bond strength and flexural strength of resin cement. The water content of the moist and dry dentins was estimated using infrared spectroscopy. Four adhesive and three self-adhesive resin cements were used. At three times of immediately, after one-day storage, and after 20,000 thermocycles (TC 20k), the shear bond strengths were measured. For all resin cements, both the shear bond strength and the flexural strength were the lowest immediately after setting; however, after one day of water storage or TC 20k, these resin cements had the highest values. Regardless of the condition of the dentin surface upon shear bond strength, the flexural strength of each resin cement was correlated with the shear bond strength of the dentin surface.

DOI： 10.4012/dmj.2021-278

新しい合着用レジン添加型グラスアイオノマーセメント接着強さと曲げ特性

入江正郎, 丸尾幸憲, 西川悟郎, 松本卓也

日本歯科理工学会誌 41 ( Special Issue79 ) 66 - 66 2022年5月

新しい合着用レジン添加型グラスアイオノマーセメント接着強さと曲げ特性

入江正郎, 丸尾幸憲, 西川悟郎, 松本卓也

日本歯科理工学会誌 41 ( Special Issue79 ) 66 - 66 2022年5月

Mechanotransductive Mechanisms of Biomimetic Hydrogel Cues Modulating Meckel's Cartilage Degeneration 査読

Mahmoud Farahat, Emilio S. Hara, Risa Anada, Gulsan A.S. Kazi, Nahid M. Akhter, Takuya Matsumoto

Advanced Biology 2101315 - 2101315 2022年3月

Self-adhering implantable device of titanium: Enhanced soft-tissue adhesion by sandblast pretreatment 査読

Atsushi Yabe, Masahiro Okada, Emilio Satoshi Hara, Yasuhiro Torii, Takuya Matsumoto

Colloids and Surfaces B: Biointerfaces 211 112283 - 112283 2022年3月

Cyclic Pressure-Induced Cytokines from Gingival Fibroblasts Stimulate Osteoclast Activity: Clinical Implications for Alveolar Bone Loss in Denture Wearers 査読

Yoshihiro Akashi, Atsuhiro Nagasaki, Hiroko Okawa, Takuya Matsumoto, Takeru Kondo, Hirofumi Yatani, Ichiro Nishimura, Hiroshi Egusa

Journal of Prosthodontic Research 2022年

Flexural Property of a Composite Biomaterial in Three Applications 査読

Masao Irie, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

JOURNAL OF COMPOSITES SCIENCE 5 ( 10 ) 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI

Resin composite is widely used in the dental field in clinics as a biomaterial. For example, it has been used as a composite material, a type of biomaterial, to repair caries and restore masticatory function, and as a luting agent to adhere the restoration to the tooth substrate. In order to demonstrate its function, we have measured the mechanical strength. From such basic research, we explain the potential of a dental material through the measurement of flexural strength and modulus of elasticity. In this research, we introduce commercial products that are actually used as composite materials suitable for tooth substrate and provide readers with their properties based on flexural strength and modulus of elasticity. In clinical performance, it might be advisable to delay polishing when a composite material is used for a luting material, a filling material and a core build-up material, as the flexural strength and the flexural modulus of elasticity were improved after 1 day of storage, and flexural strength and characteristics are considered as important mechanical properties of oral biomaterials.</p>

DOI： 10.3390/jcs5100282

新規開発の仮封材 I級窩洞辺縁部適合性と圧縮強さ

入江正郎, 丸尾幸憲, 西川悟郎, 松本卓也

日本歯科理工学会誌 40 ( Special Issue78 ) 40 - 40 2021年9月

前歯用CAD/CAMブロックに対するレジンセメントの接着強さの検討 Self-cure vs.Dual-cure

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

接着歯学 39 ( 3 ) 77 - 77 2021年9月

人工歯周組織ユニット創製のためのアパタイトコーティング法の開発

岡田正弘, 木村剛, 中村奈緒子, 岸田晶夫, 松本卓也

日本歯科理工学会誌 40 ( Special Issue78 ) 51 - 51 2021年9月

Relationships between Flexural and Bonding Properties, Marginal Adaptation, and Polymerization Shrinkage in Flowable Composite Restorations for Dental Application. 国際誌

Akimasa Tsujimoto, Masao Irie, Erica Cappelletto Nogueira Teixeira, Carlos Alberto Jurado, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto, Franklin Garcia-Godoy

Polymers 13 ( 16 ) 2021年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

To evaluate the flexural and bonding properties, marginal adaptation, and polymerization shrinkage in flowable composite restorations and their relationships, four new generation flowable composites, one conventional, and one bulk-fill flowable composite were used in this study. Flexural properties of the composites and shear bond strength to enamel and dentin for flowable restorations were measured immediately and 24 h after polymerization. Marginal adaptation, polymerization shrinkage, and stress were also investigated immediately after polymerization. The flexural properties, and bond strength of the flowable composites to enamel and dentin were much lower immediately after polymerization than at 24 h, regardless of the type of the composite. Polymerization shrinkage and stress varied depending on the material, and bulk-fill flowable composite showed much lower values than the others. The marginal adaptation and polymerization shrinkage of the composites appeared to have a much stronger correlation with a shear bond strength to dentin than to enamel. The weak mechanical properties and bond strengths of flowable composites in the early stage after polymerization must be taken into account when using them in the clinic. In addition, clinicians should be aware that polymerization shrinkage of flowable composites can still lead to the formation of gaps and failure of adaptation to the cavity regardless of the type of composite.

DOI： 10.3390/polym13162613

Immediate soft-tissue adhesion and the mechanical properties of the Ti–6Al–4V alloy after long-term acid treatment 査読国際誌

Yaming Wang, Masahiro Okada, Shi Chao Xie, Yu Yang Jiao, Emilio Satoshi Hara, Hiroaki Yanagimoto, Takumi Fukumoto, Takuya Matsumoto

Journal of Materials Chemistry B 9 ( 39 ) 8348 - 8354 2021年8月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Royal Society of Chemistry (RSC)

A metallic solid-state adhesive for biological soft tissues was fabricated using Ti–6Al–4V alloys, and the influence of the minor β phase and the small amount of Al in the α phase are reported.

DOI： 10.1039/d1tb00919b

2ステップボンディング材の初期接着強さの経時的変化

矢部淳, 入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 154回 99 - 99 2021年5月

レジンセメントの最近の歯科用セラミックスに対する接着強さの長期耐久性

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 154回 86 - 86 2021年5月

Re-Evaluation of Initial Bone Mineralization from an Engineering Perspective 査読

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takayoshi Nakano, Takuya Matsumoto

Tissue Engineering Part B: Reviews 28 ( 1 ) 246 - 255 2021年3月

Coating method for smooth and reinforced surface layer of highly translucent zirconia 査読

Yoshiro FUJII, Masahiro OKADA, Hiroaki TAKETA, Takuya MATSUMOTO

Dental Materials Journal 40 ( 2 ) 532 - 537 2021年3月

Bone Mineral Analogue Ceramic Block as an Instant Adhesive to Biological Soft Tissue (Adv. Mater. Interfaces 6/2021) 査読

Yuki Sugiura, Masahiro Okada, Ken Hirano, Takuya Matsumoto

Advanced Materials Interfaces 8 ( 6 ) 2170033 - 2170033 2021年3月

グラスアイオノマーの唾液汚染による歯質接着強さの特徴コンポジットレジンシステムとの比較

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 40 ( Special Issue77 ) 22 - 22 2021年3月

サンドブラスト処理による酸処理チタンの軟組織接着性の増強

矢部淳, 岡田正弘, 武田宏明, ハラ・エミリオ・サトシ, 鳥井康弘, 松本卓也

日本歯科理工学会誌 40 ( Special Issue77 ) 3 - 3 2021年3月

Effect of Biomechanical Environment on Degeneration of Meckel’s Cartilage 査読

M. Farahat, G.A.S. Kazi, E.S. Hara, T. Matsumoto

Journal of Dental Research 100 ( 2 ) 171 - 178 2021年2月

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：SAGE Publications

During orofacial tissue development, the anterior and posterior regions of the Meckel’s cartilage undergo mineralization, while the middle region undergoes degeneration. Despite the interesting and particular phenomena, the mechanisms that regulate the different fates of Meckel’s cartilage, including the effects of biomechanical cues, are still unclear. Therefore, the purpose of this study was to systematically investigate the course of Meckel’s cartilage during embryonic development from a biomechanical perspective. Histomorphological and biomechanical (stiffness) changes in the Meckel’s cartilage were analyzed from embryonic day 12 to postnatal day 0. The results revealed remarkable changes in the morphology and size of chondrocytes, as well as the occurrence of chondrocyte burst in the vicinity of the mineralization site, an often-seen phenomenon preceding endochondral ossification. To understand the effect of biomechanical cues on Meckel’s cartilage fate, a mechanically tuned 3-dimensional hydrogel culture system was used. At the anterior region, a moderately soft environment (10-kPa hydrogel) promoted chondrocyte burst and ossification. On the contrary, at the middle region, a more rigid environment (40-kPa hydrogel) enhanced cartilage degradation by inducing a higher expression of MMP-1 and MMP-13. These results indicate that differences in the biomechanical properties of the surrounding environment are essential factors that distinctly guide the mineralization and degradation of Meckel’s cartilage and would be valuable tools for modulating in vitro cartilage and bone tissue engineering.

DOI： 10.1177/0022034520960118

その他リンク： http://journals.sagepub.com/doi/full-xml/10.1177/0022034520960118

Flexural Property of Composite Biomaterials Used in the Oral Cavity 査読

Masao Irie, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Preprints 2021 2021年2月

Nanostructural analysis of distinct nucleation sites in pathological mineralization 査読

Emilio Satoshi Hara, Susumu Oozawa, Noriyuki Nagaoka, Masahiro Okada, Shingo Kasahara, Takuya Matsumoto

Materials Advances 2 ( 13 ) 4423 - 4431 2021年

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：Royal Society of Chemistry (RSC)

<p>Vesicular and non-vesicular phospholipids were the nucleation sites in atherosclerotic calcification. Mineralization <italic>in vitro</italic> showed that LDL, PS and PC mineralized in 2 days. PS mineralized more than PC.</p>

DOI： 10.1039/d1ma00273b

Large three-dimensional cell constructs for tissue engineering. 国際誌

Jun-Ichi Sasaki, Gabriela L Abe, Aonan Li, Takuya Matsumoto, Satoshi Imazato

Science and technology of advanced materials 22 ( 1 ) 571 - 582 2021年

記述言語：英語掲載種別：研究論文（学術雑誌）

Much research has been conducted on fabricating biomimetic biomaterials in vitro. Tissue engineering approaches are often conducted by combining cells, scaffolds, and growth factors. However, the degradation rate of scaffolds is difficult to control and the degradation byproducts occasionally limit tissue regeneration. To overcome these issues, we have developed a novel system using a thermo-responsive hydrogel that forms scaffold-free, three-dimensional (3D) cell constructs with arbitrary size and morphology. 3D cell constructs prepared using bone marrow-derived stromal stem cells (BMSCs) exhibited self-organizing ability and formed bone-like tissue with endochondral ossification. Endothelial cells were then introduced into the BMSC construct and a vessel-like structure was formed within the constructs. Additionally, the bone formation ability was promoted by endothelial cells and cell constructs could be freeze-dried to improve their clinical application. A pre-treatment with specific protein protectant allowed for the fabrication of novel bone substitutes composed only of cells. This 3D cell construct technology using thermo-responsive hydrogels was then applied to other cell species. Cell constructs composed of dental pulp stem cells were fabricated, and the resulting construct regenerated pulp-like tissue within a human pulpless tooth. In this review, we demonstrate the approaches for the in vitro fabrication of bone and dental pulp-like tissue using thermo-responsive hydrogels and their potential applications.

DOI： 10.1080/14686996.2021.1945899

Flexural Strength of Resin Core Build-Up Materials: Correlation to Root Dentin Shear Bond Strength and Pull-Out Force. 査読国際誌

Masao Irie, Yukinori Maruo, Goro Nishigawa, Kumiko Yoshihara, Takuya Matsumoto

Polymers 12 ( 12 ) 2020年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

The aims of this study were to investigate the effects of root dentin shear bond strength and pull-out force of resin core build-up materials on flexural strength immediately after setting, after one-day water storage, and after 20,000 thermocycles. Eight core build-up and three luting materials were investigated, using 10 specimens (n = 10) per subgroup. At three time periods-immediately after setting, after one-day water storage, and after 20,000 thermocycles, shear bond strengths to root dentin and pull-out forces were measured. Flexural strengths were measured using a 3-point bending test. For all core build-up and luting materials, the mean data of flexural strength, shear bond strength and pull-out force were the lowest immediately after setting. After one-day storage, almost all the materials yielded their highest results. A weak, but statistically significant, correlation was found between flexural strength and shear bond strength (r = 0.508, p = 0.0026, n = 33). As the pull-out force increased, the flexural strength of core build-up materials also increased (r = 0.398, p = 0.0218, n = 33). Multiple linear regression analyses were conducted using these three independent factors of flexural strength, pull-out force and root dentin shear bond strength, which showed this relationship: Flexural strength = 3.264 × Shear bond strength + 1.533 × Pull out force + 10.870, p = 0.002). For all the 11 core build-up and luting materials investigated immediately after setting, after one-day storage and after 20,000 thermocycles, their shear bond strengths to root dentin and pull-out forces were correlated to the flexural strength in core build-up materials. It was concluded that the flexural strength results of the core build-up material be used in research and quality control for the predictor of the shear bond strength to the root dentin and the retentive force of the post.

DOI： 10.3390/polym12122947

Micro-Architectural Investigation of Teleost Fish Rib Inducing Pliant Mechanical Property 査読

Yu Yang Jiao, Masahiro Okada, Emilio Satoshi Hara, Shi Chao Xie, Noriyuki Nagaoka, Takayoshi Nakano, Takuya Matsumoto

Materials 13 ( 22 ) 5099 - 5099 2020年11月

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Despite the fact that various reports have been discussing bone tissue regeneration, precise bone tissue manipulation, such as controlling the physical properties of the regenerated bone tissue, still remains a big challenge. Here, we focused on the teleost fish ribs showing flexible and tough mechanical properties to obtain a deeper insight into the structural and functional features of bone tissue from different species, which would be valuable for the superior design of bone-mimicking materials. Herein, we examined their compositions, microstructure, histology, and mechanical properties. The first rib of Carassius langsdorfii showed a higher Young’s modulus with a small region of chondrocyte clusters compared with other smaller ribs. In addition, highly oriented collagen fibers and osteocytes were observed in the first rib, indicating that the longest first rib would be more mature. Moreover, the layer-by-layer structure of the oriented bone collagen was observed in each rib. These microarchitectural and compositional findings of fish rib bone would give one the useful idea to reproduce such a highly flexible rib bone-like material.

DOI： 10.3390/ma13225099

新規ボンディング材の歯質接着性からの検討湿潤vs.乾燥

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 59 - 59 2020年11月

汚染除去材が接着性レジンセメントのせん断接着強さに及ぼす影響

矢部淳, 入江正郎, 岡田正弘, 野崎高儀, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 60 - 60 2020年11月

新規レジンセメントの歯質接着性からの検討各種処理材の影響

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 58 - 58 2020年11月

新規セルフアドヒーシヴ・レジンセメントの接着性からの検討プライマー併用効果、Self-cure vs.Dual-cure

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

接着歯学 38 ( 3 ) 111 - 111 2020年9月

Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis 査読

Mst Nahid Akhter, Emilio Satoshi Hara, Koichi Kadoya, Masahiro Okada, Takuya Matsumoto

International Journal of Molecular Sciences 21 ( 15 ) 5327 - 5327 2020年7月

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Current stem cell-based techniques for bone-like tissue synthesis require at least two to three weeks. Therefore, novel techniques to promote rapid 3D bone-like tissue synthesis in vitro are still required. In this study, we explored the concept of using cell nanofragments as a substrate material to promote rapid bone formation in vitro. The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. The results showed that ultrasonication for 3 min allowed the fabrication of homogeneous nanofragments of less than 150 nm in length, which mineralized surprisingly in just one day, faster than the fragments obtained from all other methods. Further optimization of culture conditions indicated that a concentration of 10 mM or 100 mM of β-glycerophosphate enhanced, whereas fetal bovine serum (FBS) inhibited in a concentration-dependent manner, the mineralization of the cell nanofragments. Finally, a 3D collagen-cell nanofragment-mineral complex mimicking a bone-like structure was generated in just two days by combining the cell nanofragments in collagen gel. In conclusion, sonication for three min could be applied as a novel method to fabricate cell nanofragments of less than 150 nm in length, which can be used as a material for in vitro bone tissue engineering.

DOI： 10.3390/ijms21155327

唾液汚染除去材処理がセラミックスの接着強さに与える影響

萬田陽介, 入江正郎, 丸尾幸憲, 西川悟郎, 吉原久美子, 長岡紀幸, 松本卓也, 皆木省吾

日本補綴歯科学会誌 12 ( 特別号 ) 171 - 171 2020年6月

ヒト血漿汚染被着面に対する汚染除去材の効果接着強さからの検討

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 152回 95 - 95 2020年6月

臼歯修復用手動練和型のグラスアイオノマーセメント I級窩洞適合性と歯質接着強さ

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 39 ( Special Issue75 ) 17 - 17 2020年4月

単斜晶ジルコニアとシリカを焼付した高透光性ジルコニアの機械的性質

岡田正弘, 宇野聡一郎, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 39 ( Special Issue75 ) 7 - 7 2020年4月

骨質指標としての骨基質配向化制御におけるオステオカルシンの役割

中野貴由, 小笹良輔, 岡田正弘, 松本卓也, 森石武史, 小守壽文

日本歯科理工学会誌 39 ( Special Issue75 ) 10 - 10 2020年4月

Toughening of highly translucent zirconia by monoclinic ZrO₂ and SiO₂ particle coating 査読

Soichiro UNO, Masahiro OKADA, Hiroaki TAKETA, Yasuhiro TORII, Takuya MATSUMOTO

Dental Materials Journal 39 ( 2 ) 295 - 301 2020年3月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Society for Dental Materials and Devices

The mechanical properties of highly translucent partially stabilized zirconia (PSZ) need to be improved; however, improvement of mechanical properties often decreases translucency. To overcome this problem, a monoclinic ZrO2 (mZrO2)/SiO2 dispersion was prepared and applied as a coating material for PSZ. The influence of surface treatment by the mZrO2/SiO2 dispersion on the surface topography, crystallography, and mechanical properties of highly translucent PSZ was investigated in this study. Following the treatment, the mechanical strength of highly translucent PSZ improved by 170% compared with control, for the best mZrO2/SiO2 dispersion ratio and heating temperature condition, while maintaining its translucency. The proposed coating is promising for improving the mechanical properties of highly translucent PSZ.

DOI： 10.4012/dmj.2018-415

Titanium as an Instant Adhesive for Biological Soft Tissue 査読

Masahiro Okada, Emilio Satoshi Hara, Atsushi Yabe, Kei Okada, Yo Shibata, Yasuhiro Torii, Takayoshi Nakano, Takuya Matsumoto

ADVANCED MATERIALS INTERFACES 2020年3月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY

A variety of polymer- and ceramic-based soft-tissue adhesives have been developed as alternatives to surgical sutures, yet several disadvantages regarding the mechanical properties, biocompatibility, and handling hinder their further application particularly when applied for immobilization of implantable devices. Here, it is reported that a biocompatible and tough metal, titanium (Ti), shows instant and remarkable adhesion properties after acid treatment, demonstrated by ex vivo shear adhesion tests with mouse dermal tissues. Importantly, in vivo experiments demonstrate that the acid-treated Ti can easily and stably immobilize a device implanted in the mouse subcutaneous tissue. Collectively, the acid-treated Ti is shown as a solid-state instant adhesive material for biological soft tissues, which can have diverse applications including immobilization of body-implantable devices.

DOI： 10.1002/admi.201902089

Vibratory splint therapy for decreasing sleep clenching: A pilot study 査読

Emilio Satoshi Hara, Andrea Lusvarghi Witzel, Hajime Minakuchi, Carlos Eduardo de Pitta, Rosane Tronquin Gallo, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Marcelo Costa Bolzan

CRANIO® 38 ( 1 ) 15 - 21 2020年1月

Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur 査読

Ryosuke Ozasa, Mayuko Nakatsu, Atsushi Moriguchi, Kyohei Sasaki, Takuya Ishimoto, Masahiro Okada, Takuya Matsumoto, Takayoshi Nakano

Materials Transactions 61 ( 2 ) 381 - 386 2020年

掲載種別：研究論文（国際会議プロシーディングス）

©2019 The Japan Institute of Metals and Materials In this study, we focused on the preferential orientation of the extracellular matrix (ECM) of bone, since ECM orientation has been shown to significantly affect the mechanical functions of bones. Bone analysis is in most cases based on the premise that the apatite crystallizes on the collagen template such that its c-axis is parallel with the running direction of the collagen fibril. Bone regeneration analysis has also been discussed assuming that the apatite c-axis orientation reflects collagen orientation. To understand the regeneration processes of both collagen and apatite individually, the preferential orientations of apatite and collagen in regenerated bone were simultaneously analyzed using a bone regeneration model of mouse femur with an 0.8-mm drill hole defect. The defects in mouse femur were filled with mineralized bone matrix, which shows an intact mineral density. However, the directions of orientation of the collagen and apatite deviate from the femur longitudinal axis in the regenerated bone. Moreover, electron diffraction analysis revealed that the apatite c-axis aligned along the extended axis of a collagen fibril both in regenerated and intact bones, indicating that the direction of the apatite c-axis is regulated by collagen fibril orientation even in the regenerated bone. In conclusion, the less-oriented apatite crystallite observed in the regenerated bone was shown to be formed due to the less-oriented collagen fibrils.

DOI： 10.2320/matertrans.MT-M2019341

Flexural properties, bond ability, and crystallographic phase of highly translucent multi-layered zirconia

Yukinori Maruo, Kumiko Yoshihara, Masao Irie, Goro Nishigawa, Noriyuki Nagaoka, Takuya Matsumoto, Shogo Minagi

Journal of Applied Biomaterials and Functional Materials 18 2020年

掲載種別：研究論文（学術雑誌）

This study investigated the mechanical properties, bond ability, and crystallographic forms of different sites in a highly translucent, multi-layered zirconia disk. Flexural properties, bond ability to resin cement, and phase composition were investigated at three sites of a highly translucent, multi-layered zirconia disk: incisal, middle, and cervical. Flexural strength (FS) and flexural modulus (FM) were measured with static three-point flexural test. Shear bond strength (SB) to resin cement was measured after 24 h storage (37°C). Phase composition under mechanical stress was analyzed using X-ray diffraction. Without air abrasion, FS at the incisal site yielded the lowest value and was significantly lower than the middle and cervical sites. Air abrasion lowered the FS of each site. FM at the incisal site without air abrasion showed the significantly lowest value, and air abrasion increased its FM value. At the middle and cervical sites, their FM values were higher than the incisal site but were not significantly affected by air abrasion. SB value did not show significant differences among the sites. After sintering, cubic zirconia was detected at each site. Rhombohedral phase transformation occurred after mirror polishing. In highly translucent, multi-layered zirconia which was mainly composed of cubic zirconia, rhombohedral phase transformation occurred under mechanical stress and resulted in weakened mechanical properties.

DOI： 10.1177/2280800020942717

唾液汚染被着面に対する汚染除去材の効果接着強さからの検討

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 151回 69 - 69 2019年10月

Fibronectin‐induced ductal formation in salivary gland self‐organization model 査読

Mahmoud Farahat, Gulsan A. S. Kazi, Hiroaki Taketa, Emilio S. Hara, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

Developmental Dynamics 248 ( 9 ) 813 - 825 2019年9月

セルフアドヒーシヴ・レジンセメント(自動練和型)の歯質接着性からの検討プライマー併用効果、Dual-cure vs.Self-cure

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

接着歯学 37 ( 3 ) 123 - 123 2019年9月

新しい練和方式の合着用レジン添加型グラスアイオノマーセメント接着強さと曲げ特性

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 38 ( Special Issue74 ) 22 - 22 2019年9月

Biomimetic mineralization using matrix vesicle nanofragments. 査読国際誌

Yosuke Kunitomi, Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takuo Kuboki, Takayoshi Nakano, Hiroshi Kamioka, Takuya Matsumoto

Journal of biomedical materials research. Part A 107 ( 5 ) 1021 - 1030 2019年5月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021-1030, 2019.

DOI： 10.1002/jbm.a.36618

最近の歯科用セラミックスに対する接着強さ

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 150回 63 - 63 2019年5月

Improvement of mechanical properties of Y-TZP by thermal annealing with monoclinic zirconia nanoparticle coating. 査読国際誌

Okada M, Taketa H, Hara ES, Torii Y, Irie M, Matsumoto T

Dental materials : official publication of the Academy of Dental Materials 35 ( 7 ) 970 - 978 2019年4月

単斜晶ナノジルコニア焼付による従来型ジルコニアの機械的性質改善

岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 38 ( Special Issue73 ) 8 - 8 2019年4月

Fabricating large-scale three-dimensional constructs with living cells by processing with syringe needles. 国際誌

Jun-Ichi Sasaki, Chihiro Katata, Gabriela L Abe, Takuya Matsumoto, Satoshi Imazato

Journal of biomedical materials research. Part A 107 ( 4 ) 904 - 909 2019年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

Three-dimensional (3D) cell constructs composed only of cells and cell-secreted extracellular matrix have been attractive biomaterials for tissue engineering technology; however, controlling construct morphology and eliminating dead cells after fabrication remain a challenge. It has been hypothesized that moderate stress could shape constructs and eliminate dead cells. The purpose of this study was to establish an easily available technology for shaping 3D cell constructs and eliminating dead cells postfabrication. To achieve these objectives, spherical cell constructs composed of L-929 fibroblasts were processed using different sized syringe needles. Our results revealed that large-scale rod-shaped cell constructs could be fabricated, and that their diameters could be controlled according to the size of the syringe needle. Additionally, cell viability assays showed that >94% of cells in the rod-shaped constructs were viable, suggesting that dead cells, which have low adhesion force, were dispersed when compressive stress was applied during passage through the needle. The technology described in this study will be promising for future tissue engineering, especially for fabricating elongated tissues such as nerves and blood vessels. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 904-909, 2019.

DOI： 10.1002/jbm.a.36613

新しい器械式練和の充填用グラスアイオノマーセメント I級窩洞適合性と歯質接着強さ

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 38 ( Special Issue73 ) 62 - 62 2019年4月

Intermediate Water on Calcium Phosphate Minerals: Its Origin and Role in Crystal Growth

Masahiro Okada, Emilio Satoshi Hara, Daisuke Kobayashi, Shoki Kai, Keiko Ogura, Masaru Tanaka, Takuya Matsumoto

ACS Applied Bio Materials 2 981 - 986 2019年3月

Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells 査読

Yuri Hazehara-Kunitomo, Emilio Hara, Mitsuaki Ono, Kyaw Thu Aung, Keiko Komi, Hai Thanh Pham, Kentaro Akiyama, Masahiro Okada, Toshitaka Oohashi, Takuya Matsumoto, Takuo Kuboki

International Journal of Molecular Sciences 20 ( 5 ) 2019年3月

Optimal sandblasting conditions for conventional-type yttria-stabilized tetragonal zirconia polycrystals. 査読国際誌

Okada M, Taketa H, Torii Y, Irie M, Matsumoto T

Dental materials : official publication of the Academy of Dental Materials 35 ( 1 ) 169 - 175 2019年1月

Fabrication of single gel with different mechanical stiffness using three-dimensional mold. 査読

Kazi GAS, Rahman KA, Farahat M, Matsumoto T

Journal of biomedical materials research. Part A 107 ( 1 ) 6 - 11 2019年1月

新規表面処理法が高透光性ジルコニアの機械的性質および接着性に及ぼす影響

武田宏明, 岡田正弘, 宇野聡一郎, 入江正郎, 鳥井康弘, 松本卓也

岡山歯学会雑誌 37 ( 2 ) 84 - 84 2018年12月

最近のフロアブルコンポジットの重合挙動と窩洞適合性

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 149回 64 - 64 2018年10月

高透光性ジルコニアに対する機械的表面処理条件の検討

岡田正弘, 藤井惠朗, 宇野聡一郎, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 37 ( Special Issue72 ) 35 - 35 2018年9月

ワンステップ接着システムにおける歯質界面の生成物と物性の関係

鈴木一臣, 長岡紀幸, 吉原久美子, 松本卓也, 吉田靖弘

日本歯科理工学会誌 37 ( Special Issue72 ) 29 - 29 2018年9月

下顎偏位時における舌の三次元的位置解析

徳善貴大, 兒玉直紀, 長塚弘亮, 村上明日香, 田中祐貴, 松本卓也, 皆木省吾

日本補綴歯科学会誌 10 ( 特別号 ) 260 - 260 2018年6月

レジンセメントの大臼歯用CAD/CAM Blockに対するせん断接着強さ

入江正郎, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 148回 61 - 61 2018年5月

高透光性ジルコニアの機械的強さに及ぼす表面処理の影響

大島直也, 岡田正弘, 宇野聡一郎, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 37 ( Special Issue71 ) 38 - 38 2018年3月

Bioinspired Mineralization Using Chondrocyte Membrane Nanofragments

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

ACS Biomaterials Science & Engineering 4 617 - 625 2018年2月

Bioinspired Mineralization Using Chondrocyte Membrane Nanofragments 査読

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

ACS Biomaterials Science & Engineering 4 ( 2 ) 617 - 625 2018年2月

Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu.

Hara ES, Okada M, Kuboki T, Nakano T, Matsumoto T

J Mater Chem B. 6 ( 38 ) 6153 - 6161 2018年

Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu 査読

Emilio Satoshi Hara, Masahiro Okada, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

Journal of Materials Chemistry B 6 ( 38 ) 6153 - 6161 2018年

Chondrocyte burst promotes space for mineral expansion 査読

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Letycia Mary Iida, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

Integrative Biology 10 ( 1 ) 57 - 66 2018年

Fabrication of Hydroxyapatite Nanofibers with High Aspect Ratio via Low-Temperature Wet Precipitation Methods Under Acidic Conditions

Masahiro Okada, Emilio Satoshi Hara, Takuya Matsumoto

Biomineralization 211 - 218 2018年

The effect of Mg and Sr on the crystallinity of bones evaluated through Raman spectroscopy and laser ablation-ICPMS analysis 査読

Yu-ki Tanaka, Nobuyuki Yajima, Masahiro Okada, Takuya Matsumoto, Yusuke Higuchi, Shigeaki Miyazaki, Hideyuki Yamato, Takafumi Hirata

ANALYST 142 ( 22 ) 4265 - 4278 2017年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ROYAL SOC CHEMISTRY

We investigated the possible linkage between the crystallinity and elemental ratios (Mg/Ca and Sr/Ca) of the femoral cortical bones of rats with chronic kidney disease (CKD) or diabetes mellitus (DM). The Mg/Ca and Sr/Ca ratios were measured by using the laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) technique and the crystallinity was evaluated by Raman spectroscopy on the same sample slice. The measured crystallinity varied significantly along the radial direction, reflecting the heterogeneities in the Mg/Ca ratio for the bone samples. For the endosteal cortical bone of CKD rats, the Mg/Ca ratio became higher. This is explained by the increase of the abundance of Mg in the bone, possibly due to the higher absorption efficiency of Mg in the intestine or due to the lower excretion efficiency of Mg from the kidney. For areas with a higher Mg/Ca ratio, the crystallinity of the bone was significantly more degraded than that for areas with a lower Mg/Ca ratio, suggesting that the substitution of Ca by Mg induced the deterioration of the crystallinity of hydroxyapatite (HAp). In addition, the measured Mg/Ca and Sr/Ca ratios of the bone were positively correlated with those found in serum samples. The data obtained here demonstrated that the metabolic alterations for inorganic elements caused the ionic substitutions of Ca by foreign cationic ions, and that the contents of foreign ions in the bone greatly affected the crystallinity of HAp.

DOI： 10.1039/c7an00734e

Resin adhesion strengths to zirconia ceramics after primer treatment with silane coupling monomer or oligomer 査読

Masahiro Okada, Kazusa Inoue, Masao Irie, Hiroaki Taketa, Yasuhiro Torii, Takuya Matsumoto

DENTAL MATERIALS JOURNAL 36 ( 5 ) 600 - 605 2017年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Resin bonding to zirconia ceramics is difficult to achieve using the standard methods for conventional silica-based dental ceramics, which employ silane coupling monomers as primers. The hypothesis in this study was that a silane coupling oligomer a condensed product of silane coupling monomers would be a more suitable primer for zirconia. To prove this hypothesis, the shear bond strengths between a composite resin and zirconia were compared after applying either a silane coupling monomer or oligomer. The shear bond strength increased after applying a non-activated ethanol solution of the silane coupling oligomer compared with that achieved when applying the monomer. Thermal treatment of the zirconia at 110 degrees C after application of the silane coupling agents was essential to improve the shear bond strength between the composite resin cement and zirconia.

DOI： 10.4012/dmj.2016-334

最近のフロアブルコンポジットの歯質接着性と曲げ特性

入江正郎, 徳永英里, 丸尾幸憲, 西川悟郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 35 ( 3 ) 77 - 77 2017年10月

歯根象牙質と直接法レジンコアー材料との接着性耐久性

西川悟郎, 入江正郎, 丸尾幸憲, 徳永英里, 吉原久美子, 長岡紀幸, 松本卓也, 皆木省吾

接着歯学 35 ( 3 ) 79 - 79 2017年10月

ファイバーポストとコアー材の引き抜き強さ

入江正郎, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 147回 148 - 148 2017年10月

歯科用ジルコニアへのサンドブラスト処理の影響

岡田正弘, 武田宏明, 鳥井康弘, 入江正郎, 松本卓也

日本歯科理工学会誌 36 ( 5 ) 327 - 327 2017年9月

Biocompatible nanostructured solid adhesives for biological soft tissues 査読

Masahiro Okada, Akira Nakai, Emilio Satoshi Hara, Tetsushi Taguchi, Takayoshi Nakano, Takuya Matsumoto

Acta Biomaterialia 57 404 - 413 2017年7月

ファイバーポストとコアー材の初期の引き抜き強さ

入江正郎, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 146回 91 - 91 2017年5月

MSCs feeder layers induce SMG self-organization and branching morphogenesis 査読

Mahmoud Farahat, Gulsan Ara Sathi, Emilio Satoshi Hara, Hiroaki Taketa, Takuo Kuboki, Takuya Matsumoto, Masaya Yamamoto

PLOS ONE 12 ( 4 ) e0176453 2017年4月

MCSF orchestrates branching morphogenesis in developing submandibular gland tissue 査読

Gulsan Ara Sathi, Mahmoud Farahat, Emilio Satoshi Hara, Hiroaki Taketa, Hitoshi Nagatsuka, Takuo Kuboki, Takuya Matsumoto

Journal of Cell Science 130 ( 9 ) 1559 - 1569 2017年3月

Does 8-methacryloxyoctyl trimethoxy silane(8-MOTS) improve initial bond strength on lithium disilicate glass ceramic? 査読

Yukinori Maruo, Goro Nishigawa, Kumiko Yoshihara, Shogo Minagi, Takuya Matsumoto, Masao Irie

DENTAL MATERIALS 33 ( 3 ) E95 - E100 2017年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Objectives. Dental ceramic surfaces are modified with silane coupling agents, such as gamma-ymethacryloxypropyl trimethoxy silane (gamma-MPTS), to improve bond strength. For bonding between lithium disilicate glass ceramic and resin cement, the objective was to investigate if 8-methacryloxyoctyl trimethoxy silane (8 -MOTS) could yield a similar performance as the widely used gamma-MPTS.
Methods. One hundred and ten lithium disilicate glass ceramic specimens were randomly divided into 11 groups (n = 10) according to pretreatment regime. All specimens were pre-treated with a different solution composed of one or a combination of these agents: 10 or 20 wt% silane coupling agent of gamma-MPTS or 8 -MOTS, followed by a hydrolysis solution of acetic acid or 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP). Each pretreated surface was luted to a stainless steel rod of 3.6 mm diameter and 2.0 mm height with resin cement. Shear bond strength between ceramic and cement was measured after 24-h storage in 37 degrees C distilled water.
Results. 8 -MOTS produced the same bonding performance as gamma-MPTS. Both silane coupling agents significantly increased the bond strength of resin cement, depending on their concentration. When activated by 10-MDP hydrolysis solution, 20 wt% concentration produced the highest values (gamma-MPTS: 24.9 +/- 5.1 MPa; 8 -MOTS: 24.6 +/- 7.4 MPa). Hydrolysis with acetic acid produced lower bond strengths than with 10-MDP.
Significance. Silane coupling pretreatment with 8 -MOTS increased the initial bond strength between lithium disilicate glass ceramic and resin cement, rendering the same bonding effect as the conventional gamma-MPTS. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.dental.2016.11.004

ジルコニアの曲げ強さに与える表面処理の影響

岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 36 ( 2 ) 67 - 67 2017年3月

Does acid etching morphologically and chemically affect lithium disilicate glass ceramic surfaces? 査読

Yukinori Maruo, Goro Nishigawa, Masao Irie, Kumiko Yoshihara, Takuya Matsumoto, Shogo Minagi

JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS 15 ( 1 ) E93 - E100 2017年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WICHTIG PUBLISHING

Background: This study evaluated the surface morphology, chemical composition and adhesiveness of lithium disilicate glass ceramic after acid etching with hydrofluoric acid or phosphoric acid.
Methods: Lithium disilicate glass ceramic specimens polished by 600-grit silicon carbide paper were subjected to one or a combination of these surface treatments: airborne particle abrasion with 50-mu m alumina (AA), etching with 5% hydrofluoric acid (HF) or 36% phosphoric acid (Phos), and application of silane coupling agent (Si). Stainless steel rods of 3.6-mm diameter and 2.0-mm height were cemented onto treated ceramic surfaces with a self-adhesive resin cement (Clearfil SA Cement). Shear bond strengths between ceramic and cement were measured after 24-hour storage in 37 degrees C distilled water.
Results: SEM images of AA revealed the formation of conventional microretentive grooves, but acid etching with HF or Phos produced a porous surface. Bond strengths of AA+HF+Si (28.1 +/- 6.0 MPa), AA+Phos+Si (17.5 +/- 4.1 MPa) and HF+Si (21.0 +/- 3.0 MPa) were significantly greater than those of non-pretreated controls with Si (9.7 +/- 3.7 MPa) and without Si (4.1 +/- 2.4 MPa) (p<0.05). In addition, HF etching alone (26.2 +/- 7.5 MPa) had significantly higher bond strength than AA alone (11.5 +/- 4.0 MPa) (p<0.05). AA+HF, AA+Phos and HF showed cohesive failures.
Conclusions: Etching with HF or Phos yielded higher bond strength between lithium disilicate glass ceramic and self-adhesive resin cement without microcrack formation.

DOI： 10.5301/jabfm.5000303

Fabrication of Artificial Food Bolus for Evaluation of Swallowing 査読

Miyu Hosotsubo, Tetsuro Magota, Masahiko Egusa, Takuya Miyawaki, Takuya Matsumoto

PLOS ONE 11 ( 12 ) e0168378 2016年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PUBLIC LIBRARY SCIENCE

Simple and easy methods to evaluate swallowing are required because of the recently increased need of rehabilitation for dysphagia. "Artificial food bolus", but not "artificial food", would be a valuable tool for swallowing evaluation without considering the mastication effect which is altered according to the individual's oral condition. Thus, this study was carried out to fabricate artificial bolus resembling natural food bolus. The mechanical property and the volume change of food bolus in normal people were firstly investigated. Thirty healthy adults without dysphagia were selected and asked to chew four sample foods (rice cake, peanut, burdock, and gummy candy). The results indicated that Young's modulus of bolus before swallowing was below 150 kPa. The bolus volume before swallowing was below 400 mm(3). In addition, the saliva component ratio of each bolus was approximately 30wt%, and the average saliva viscosity of research participants was approximately 10 mPa.s. Based on the obtained data, artificial food bolus was designed and fabricated by using alginate hydrogel as a visco-elastic material and gelatin solution as a viscotic material with a ratio of 7: 3 based on weight. Consequently, the swallowing time of fabricated artificial food bolus was measured among the same participants. The results indicated the participants swallowed fabricated food bolus with similar manner reflecting their mechanical property and volume. Thus, this artificial food bolus would be a promising tool for evaluation of swallowing.

DOI： 10.1371/journal.pone.0168378

最近の合着用セメント象牙質とCAD/CAM用レジンブロックに対する接着強さ

入江正郎, 飯田祥与, 丸尾幸憲, 西川悟郎, 皆木省吾, 長岡紀幸, 吉原久美子, 松本卓也

接着歯学 34 ( 4 ) 141 - 149 2016年12月

記述言語：日本語出版者・発行元：(一社)日本接着歯学会

6種の接着システム[3種の処理材併用のレジンセメント;RelyX Ultimate(3M)、Resicem(Shofu)、ESTECEM(Tokuyama Dental)、2種の処理材を併用しないレジンセメント;G-Cem Cerasmart(GC)、SA Luting Plus(Kuraray Noritake Dental)、および1種のレジン配合型グラスアイオノマーセメント;Resiglass(Shofu)]を使用して硬化直後と1日後の象牙質に対する接着強さを検討、次に6種のCAD/CAM冠用レジンブロック[Lava Ultimate(3M)、Cerasmart(GC)、Shofu HC Block(Shofu)、ESTELITE BLOCK(Tokuyama Dental)、KZR-CAD HR(Yamakin)、KATANA Avencia (Kuraray Noritake Dental)]に対して1日後の被着面のサンドブラスト処理有無の影響も接着強さから検討した。その結果、象牙質に対する接着強さは、いずれのセメントも1日後の方が、硬化直後と比較して有意に高い値を示した(t-Test、p<0.05)。1日後のCAD/CAM冠用レジンブロックに対する接着強さは、4種の接着システム(Lava UltimateにScotchbond Universal Adhesive/RelyX Ultimateで接着させた場合、CerasmartにCeramic Primer II/G-Cem Cerasmartで接着させた場合、ESTELITE BLOCKにUNIVERSAL PRIMER/ESTECEMで接着させた場合、KZR-CAD HRにScotchbond Universal Adhesive/RelyX Ultimateで接着させた場合)にサンドブラスト処理することで有意に高い値を示した(t-Test、p<0.05)。しかし、それ以外の3種の接着システム(Shofu HC BlockにPorcelain Primer/Resicemで接着させた場合、Shofu HC BlockにResiglassで接着させた場合、KATANA AvenciaにClearfil Ceramic Primer/SA Luting Plusで接着させた場合)はサンドブラスト処理しても有意に高い値を示さなかった(t-Test、p>0.05)。(著者抄録)

プライマーの併用が可能な新規セルフアドヒーシブ・レジンセメントの歯質接着性と曲げ特性

西川悟郎, 飯田祥与, 丸尾幸憲, 入江正郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 34 ( 3 ) 113 - 113 2016年11月

新規動揺歯固定材の歯質接着性と曲げ特性

入江正郎, 飯田祥与, 丸尾幸憲, 西川悟郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 34 ( 3 ) 117 - 117 2016年11月

Adsorption and desorption behaviors of cetylpyridinium chloride on hydroxyapatite nanoparticles with different morphologies 査読

Masahiro Okada, Daisuke Hiramatsu, Takumi Okihara, Takuya Matsumoto

DENTAL MATERIALS JOURNAL 35 ( 4 ) 651 - 658 2016年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Application of hydroxyapatite (HAp) nanoparticles to repair damaged enamel has attracted recent attention. In this study, HAp nanoparticles with various morphologies (spherical, short-rod, long-rod and fiber morphologies) were synthesized via chemical precipitation methods without the addition of template molecules, and the adsorption/desorption behaviors of a cationic antibacterial agent, cetylpyridinium chloride (CPC), on the HAp nanoparticles were evaluated. The adsorption of CPC on each HAp nanoparticle showed Langmuir-type adsorption, and the short-rod/long-rod HAp nanoparticles showed thermodynamically more stable adsorption of CPC than that with the spherical/fiber HAp nanoparticles. The desorption rate of CPC from the short-rod/long-rod HAp nanoparticles was slower than that of the spherical/fiber HAp nanoparticles. The HAp nanoparticles with different CPC release profiles presented here have potential applications as nanoparticulate enamel repair agents with antibacterial properties.

DOI： 10.4012/dmj.2015-420

Highly dynamic self-organizing SMG morphogenesis in 3D culture 査読

Mahmoud Farahat, Gulsan Ara Sathi, Hiroaki Taketa, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 1 - 3 2016年3月

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：Institute of Electrical and Electronics Engineers Inc.

Biological tissues have specific cell patterns and well-organized structures which result from various organogenesis processes. Among which is branching morphogenesis which is the key developmental processes for many glandular tissues including lungs, kidney, salivary gland and liver. Many approaches were suggested to regenerate salivary gland tissue in vitro. Here, we developed a 3D culture technique to study and recapitulate the dynamic forces generated during SMG organogenesis by utilizing time lapse live imaging to monitor the cellular migration and reorganization process of both epithelium and mesenchyme cells.

DOI： 10.1109/MHS.2015.7438259

Functional peptide KP24 enhances submandibular gland tissue growth in vitro

Atsushi Ikeda, Hiroaki Taketa, Gulsan Ara Sathi, Yoshiaki Hirano, Seiji Iida, Takuya Matsumoto

REGENERATIVE THERAPY 3 108 - 113 2016年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Introduction: Salivary gland hypofunction, also known as xerostomia, occurs as a result of radiotherapy for head and neck cancer, autoimmune diseases, or aging. Xerostomia leads to oral health problems and thus affects the quality of life. Biological salivary gland tissue generated in vitro would provide an alternative mode of treatment for this disease.
Methods: To develop a novel method for modulating salivary gland tissue growth in vitro, we prepared a KP24 peptide-immobilized hydrogel sheet, wherein the peptide comprised repeating proline and lysine sequences, and evaluated the effect of this peptide on salivary gland tissue growth.
Results: We found that the KP24 peptide has the potential to enhance glandular tissue growth in vitro. This enhancement is associated with neurite outgrowth and increasing neural innervation.
Conclusion: KP24 peptide modified material would be a promising material for the modulation of salivary gland tissue growth in vitro. (C) 2016, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.

DOI： 10.1016/j.reth.2016.02.006

Various Effects of Sandblasting of Dental Restorative Materials 査読

Goro Nishigawa, Yukinori Maruo, Masao Irie, Naoto Maeda, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Shogo Minagi

PLOS ONE 11 ( 1 ) e0147077 2016年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PUBLIC LIBRARY SCIENCE

Background
Sandblasting particles which remain on the surfaces of dental restorations are removed prior to cementation. It is probable that adhesive strength between luting material and sandblasting particle remnants might exceed that with restorative material. If that being the case, blasting particles adhere to sandblasted material surface could be instrumental to increasing adhesive strength like underlying bonding mechanism between luting material and silanized particles of tribochemical silica coating-treated surface. We hypothesize that ultrasonic cleaning of bonding surfaces, which were pretreated with sandblasting, may affect adhesive strength of a resin luting material to dental restorative materials.
Methods
We therefore observed adhesive strength of resin luting material to aluminum oxide was greater than those to zirconia ceramic and cobalt-chromium alloy beforehand. To measure the shear bond strengths of resin luting material to zirconia ceramic and cobalt-chromium alloy, forty specimens of each restorative material were prepared. Bonding surfaces were polished with silicon abrasive paper and then treated with sandblasting. For each restorative material, 40 sandblasted specimens were equally divided into two groups: ultrasonic cleaning (USC) group and non-ultrasonic cleaning (NUSC) group. After resin luting material was polymerized on bonding surface, shear test was performed to evaluate effect of ultrasonic cleaning of bonding surfaces pretreated with sandblasting on bond strength.
Results
For both zirconia ceramic and cobalt-chromium alloy, NUSC group showed significantly higher shear bond strength than USC group.
Conclusions
Ultrasonic cleaning of dental restorations after sandblasting should be avoided to retain improved bonding between these materials.

DOI： 10.1371/journal.pone.0147077

Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells 査読

Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

STEM CELLS INTERNATIONAL 2016 6240794 2016年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：HINDAWI LTD

Three-dimensional (3D) cell constructs are expected to provide osteoinductive materials to develop cell-based therapies for bone regeneration. The proliferation and spontaneous aggregation capability of induced pluripotent stemcells (iPSCs) thus prompted us to fabricate a scaffold-free iPSC construct as a transplantation vehicle. Embryoid bodies of mouse gingival fibroblast-derived iPSCs (GF-iPSCs) were seeded in a cell chamber with a round-bottom well made of a thermoresponsive hydrogel. Collected ball-like cell constructs were cultured in osteogenic induction medium for 30 days with gentle shaking, resulting in significant upregulation of osteogenic marker genes. The constructs consisted of an inner region of unstructured cell mass and an outer osseous tissue region that was surrounded by osteoblast progenitor-like cells. The outer osseous tissue was robustly calcified with elemental calcium and phosphorous as well as hydroxyapatite. Subcutaneous transplantation of the GF-iPSC constructs into immunodeficient mice contributed to extensive ectopic bone formation surrounded by teratoma tissue. These results suggest that mouse GF-iPSCs could facilitate the fabrication of osteoinductive scaffold-free 3D cell constructs, in which the calcified regions and surrounding osteoblasts may function as scaffolds and drivers of osteoinduction, respectively. With incorporation of technologies to inhibit teratoma formation, this system could provide a promising strategy for bone regenerative therapies.

DOI： 10.1155/2016/6240794

Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells 査読

Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

Stem Cells International 2016 2016年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Hindawi Limited

Three-dimensional (3D) cell constructs are expected to provide osteoinductive materials to develop cell-based therapies for bone regeneration. The proliferation and spontaneous aggregation capability of induced pluripotent stem cells (iPSCs) thus prompted us to fabricate a scaffold-free iPSC construct as a transplantation vehicle. Embryoid bodies of mouse gingival fibroblast-derived iPSCs (GF-iPSCs) were seeded in a cell chamber with a round-bottom well made of a thermoresponsive hydrogel. Collected ball-like cell constructs were cultured in osteogenic induction medium for 30 days with gentle shaking, resulting in significant upregulation of osteogenic marker genes. The constructs consisted of an inner region of unstructured cell mass and an outer osseous tissue region that was surrounded by osteoblast progenitor-like cells. The outer osseous tissue was robustly calcified with elemental calcium and phosphorous as well as hydroxyapatite. Subcutaneous transplantation of the GF-iPSC constructs into immunodeficient mice contributed to extensive ectopic bone formation surrounded by teratoma tissue. These results suggest that mouse GF-iPSCs could facilitate the fabrication of osteoinductive scaffold-free 3D cell constructs, in which the calcified regions and surrounding osteoblasts may function as scaffolds and drivers of osteoinduction, respectively. With incorporation of technologies to inhibit teratoma formation, this system could provide a promising strategy for bone regenerative therapies.

DOI： 10.1155/2016/6240794

レジンセメントのCAD/CAM用レジンブロックに対する接着強さに及ぼすサンドブラスト処理の影響

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 皆木省吾, 吉原久美子

接着歯学 33 ( 4 ) 181 - 186 2015年12月

記述言語：日本語出版者・発行元：(一社)日本接着歯学会

接着性レジンセメントシステムRelyX Ultimate/Scotchbond Universal Adhesive(3M)の、5種CAD/CAM冠用レジンブロック(Lava Ultimate:3M、CeraSmart:ジーシー、松風HC Block:松風、KZR-CAD HR:山本貴金属地金、KATANA Avencia:クラレノリタケデンタル)に対する接着強さに及ぼすサンドブラストおよびコジェット・サンドブラスト処理の影響を検討した。その結果、サンドブラストあるいはコジェット・サンドブラスト処理することにより、無処理と比較して全てのブロックにおいて有意に接着強さは向上した。ただし、サンドブラスト処理が有効か、コジェット・サンドブラスト処理が有効かは、それぞれのCAD/CAM冠用レジンブロックによって異なることが示された。(著者抄録)

咀嚼による食材の物性変化と嚥下との関係

細坪充裕, 江草正彦, 宮脇卓也, 松本卓也

日本摂食・嚥下リハビリテーション学会雑誌 19 ( 3 ) S287 - S287 2015年12月

Synthesis and modification of apatite nanoparticles for use in dental and medical applications 査読

Masahiro Okada, Takuya Matsumoto

Japanese Dental Science Review 51 ( 4 ) 85 - 95 2015年11月

記述言語：英語出版者・発行元：Elsevier Ltd

Synthesised hydroxyapatite (HAp) exhibits excellent biocompatibility, making it an ideal candidate for use as a hard tissue substitute material. Nanoscale-size effects and surface phenomena impart HAp nanoparticles with unique properties compared to the conventional-sized HAp ceramics. Modification of HAp is also important for regulating its physiochemical properties. In this review, methods of HAp synthesis and modification, and various applications of HAp nanoparticles for dental and medical treatment are discussed.

DOI： 10.1016/j.jdsr.2015.03.004

新規動揺歯固定および矯正用ブラケット接着材のエナメル質に対する接着強さ

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 皆木省吾, 吉原久美子, 長岡紀幸

接着歯学 33 ( 3 ) 143 - 143 2015年11月

最近のレジンセメントの歯質接着性と曲げ特性

丸尾幸憲, 入江正郎, 西川悟郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 33 ( 3 ) 142 - 142 2015年11月

Fluocinolone acetonideとTGF-β3を用いた強力な軟骨再生(Potent cartilage regeneration with fluocinolone acetonide and TGF-β3)

Hara Emilio Satoshi, 大野充昭, Pham Hai Thanh, 久保田聡, 滝川正春, Young Marian F., Olsen Bjorn R., 松本卓也, 窪木拓男

日本バイオマテリアル学会大会予稿集 37回 209 - 209 2015年11月

充填用グラスアイオノマーセメント I級窩洞適合性と歯質接着強さ

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 皆木省吾, 長岡紀幸, 吉原久美子

日本歯科理工学会誌 34 ( 5 ) 407 - 407 2015年9月

Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro. 査読国際誌

Hiroaki Taketa, Gulsan Ara Sathi, Mahmoud Farahat, Kazi Anisur Rahman, Takayoshi Sakai, Yoshiaki Hirano, Takuo Kuboki, Yasuhiro Torii, Takuya Matsumoto

Scientific reports 5 11468 - 11468 2015年6月

記述言語：英語掲載種別：研究論文（学術雑誌）

In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication.

DOI： 10.1038/srep11468

Early Initiation of Endochondral Ossification of Mouse Femur Cultured in Hydrogel with Different Mechanical Stiffness 査読

Gulsan Ara Sathi, Kodai Kenmizaki, Satoshi Yamaguchi, Hitoshi Nagatsuka, Yasuhiro Yoshida, Aira Matsugaki, Takuya Ishimoto, Satoshi Imazato, Takayoshi Nakano, Takuya Matsumoto

TISSUE ENGINEERING PART C-METHODS 21 ( 6 ) 567 - 575 2015年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

Mineralization is one of the most important processes in normal bone tissue development and in disease condition. Developing a novel and standardized in vitro model system that can readily monitor both cellular dynamics and mineralization is crucial for better understanding the bone tissue development and growth. Recent studies indicated that the mechanical environment is a critical condition in mineralization. We hypothesized that hydrogel with different mechanical stiffness can provide a biomimetic mechanical environment that can modulate bone tissue growth and mineralization. A femur of mouse embryo (embryonic day 16) was embedded in agarose hydrogel (2-60 kPa) and cultured in an osteogenic medium for a week. Microcomputed tomography (mu CT) results revealed enhanced mineralization was detected in the femur head cultured in the gel condition, whereas no mineralization in the femur head cultured in the control (floating culture) condition. The mineralized region was corresponding to the region of secondary ossification center. Both histological and quantitative analyses indicated that the mineralized region of femur head cultured in 10 kPa gel condition was the highest and the mineralized area was significantly larger than that cultured in 2, 40, and 60 kPa gel condition. Immunofluorescence results indicated the enhanced mineralization caused by the higher chondrogenic differentiation at that region. This enhancement mainly relating to the mechanical forces and not to the oxygen tension was also confirmed. Since this system enhances and shortens the mineralization procedure compared with the conventional two-dimensional or three-dimensional cell culture system, this hydrogel system would be one of the unique models for better understanding the mineralized tissue development.

DOI： 10.1089/ten.tec.2014.0475

Fabrication of Biomimetic Bone Tissue Using Mesenchymal Stem Cell-Derived Three-Dimensional Constructs Incorporating Endothelial Cells 査読

Jun-Ichi Sasaki, Masanori Hashimoto, Satoshi Yamaguchi, Yoshihiro Itoh, Itsumi Yoshimoto, Takuya Matsumoto, Satoshi Imazato

PLOS ONE 10 ( 6 ) e0129266 2015年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PUBLIC LIBRARY SCIENCE

The development of technologies to promote vascularization of engineered tissue would drive major developments in tissue engineering and regenerative medicine. Recently, we succeeded in fabricating three-dimensional (3D) cell constructs composed of mesenchymal stem cells (MSCs). However, the majority of cells within the constructs underwent necrosis due to a lack of nutrients and oxygen. We hypothesized that incorporation of vascular endothelial cells would improve the cell survival rate and aid in the fabrication of biomimetic bone tissues in vitro. The purpose of this study was to assess the impact of endothelial cells combined with the MSC constructs (MSC/HUVEC constructs) during short-and long-term culture. When human umbilical vein endothelial cells (HUVECs) were incorporated into the cell constructs, cell viability and growth factor production were increased after 7 days. Furthermore, HUVECs were observed to proliferate and self-organize into reticulate porous structures by interacting with the MSCs. After long-term culture, MSC/HUVEC constructs formed abundant mineralized matrices compared with those composed of MSCs alone. Transmission electron microscopy and qualitative analysis revealed that the mineralized matrices comprised porous cancellous bone-like tissues. These results demonstrate that highly biomimetic bone tissue can be fabricated in vitro by 3D MSC constructs incorporated with HUVECs.

DOI： 10.1371/journal.pone.0129266

Early Initiation of Endochondral Ossification of Mouse Femur Cultured in Hydrogel with Different Mechanical Stiffness 査読

Gulsan Ara Sathi, Kodai Kenmizaki, Satoshi Yamaguchi, Hitoshi Nagatsuka, Yasuhiro Yoshida, Aira Matsugaki, Takuya Ishimoto, Satoshi Imazato, Takayoshi Nakano, Takuya Matsumoto

TISSUE ENGINEERING PART C-METHODS 21 ( 6 ) 567 - 575 2015年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

Mineralization is one of the most important processes in normal bone tissue development and in disease condition. Developing a novel and standardized in vitro model system that can readily monitor both cellular dynamics and mineralization is crucial for better understanding the bone tissue development and growth. Recent studies indicated that the mechanical environment is a critical condition in mineralization. We hypothesized that hydrogel with different mechanical stiffness can provide a biomimetic mechanical environment that can modulate bone tissue growth and mineralization. A femur of mouse embryo (embryonic day 16) was embedded in agarose hydrogel (2-60 kPa) and cultured in an osteogenic medium for a week. Microcomputed tomography (mu CT) results revealed enhanced mineralization was detected in the femur head cultured in the gel condition, whereas no mineralization in the femur head cultured in the control (floating culture) condition. The mineralized region was corresponding to the region of secondary ossification center. Both histological and quantitative analyses indicated that the mineralized region of femur head cultured in 10 kPa gel condition was the highest and the mineralized area was significantly larger than that cultured in 2, 40, and 60 kPa gel condition. Immunofluorescence results indicated the enhanced mineralization caused by the higher chondrogenic differentiation at that region. This enhancement mainly relating to the mechanical forces and not to the oxygen tension was also confirmed. Since this system enhances and shortens the mineralization procedure compared with the conventional two-dimensional or three-dimensional cell culture system, this hydrogel system would be one of the unique models for better understanding the mineralized tissue development.

DOI： 10.1089/ten.tec.2014.0475

Biodental engineering 査読

Takuya Matsumoto, Hiroshi Egusa, Koichi Kato, Takashi Tsuji

Journal of Oral Biosciences 57 ( 2 ) 80 - 85 2015年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Association for Oral Biology

Background Resoluble questions are estimated to mostly be resolved. Consequently, most of the remaining questions are those for which one has given up in looking for solutions
this may currently be the biggest problem for biological research. Therefore, for the continued advancement of biological sciences, it will be necessary to integrate current biological research with other fields, including chemistry, physics, robotics, material sciences, computer sciences, and mathematics. Highlight Integrated research will provide new insights into basic biology, as well as new technological outputs. Conclusion The newly emerging field of biodental engineering is one such example of integrated research with tremendous potential for facilitating future dental science research.

DOI： 10.1016/j.job.2015.01.002

Biodental engineering 査読

Matsumoto Takuya, Egusa Hiroshi, Kato Koichi, Tsuji Takashi

JOURNAL OF ORAL BIOSCIENCES 57 ( 2 ) 80 - 85 2015年5月

CAD/CAMレジンブロックとレジンセメントの歯質接着性に関する研究

飯田祥与, 入江正郎, 西川悟郎, 丸尾幸憲, 吉原久美子, 前田直人, 荒木大介, 萬田陽介, 松本卓也, 皆木省吾

日本補綴歯科学会誌 7 ( 特別号 ) 263 - 263 2015年5月

1液性ボンディング材中に含まれるシランカップリング材のカップリング効果の検討

吉原久美子, 長岡紀幸, 入江正郎, 西川悟郎, 丸尾幸憲, 松本卓也, 皆木省吾, 吉田靖弘

日本補綴歯科学会誌 7 ( 特別号 ) 169 - 169 2015年5月

Novel 3D-Printed Device for the Simple Preparation of Hydrogel Beads 査読

Kazi Anisur Rahman, Gulsan Ara Sathi, Hiroaki Taketa, Mahmoud Farahat, Masahiro Okada, Yasuhiro Torii, Takuya Matsumoto

3D PRINTING AND ADDITIVE MANUFACTURING 2 ( 1 ) 5 - 11 2015年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

Encapsulation of cells in biocompatible polymers such as naturally derived hydrogels is an important technology that is used for cell therapy and tissue engineering. Herein, we introduce a simple and novel 3D-printed device to prepare hydrogel beads. The device is composed of a cylindrical tube with 1 inlet and 16 outlets. For the hydrogel solution, a low-melting-point agarose solution and sodium alginate are used. At a solution flow rate of 250 mu L/min and a stir speed of 500, 1000, or 1250 rpm, the device produced beads of diameters 50-100, 30-60, and 5-30 mu m, respectively. When the device was run at a flow rate of 500 mu L/min at the above-mentioned stir speeds, the bead diameters were 100-150, 60-90, and 30-60 mu m, respectively. Thus, this device can produce hydrogel beads of different sizes by altering the stir speed or the solution flow rate. In addition, we demonstrated that encapsulated MC3T3-E1 cells can survive and proliferate in the fabricated hydrogel when type I collagen is added to the gel solution. The simplicity of its design would make this device a valuable tool for rapid fabrication of cell encapsulated hydrogels.

DOI： 10.1089/3dp.2014.0023

Novel 3D-Printed Device for the Simple Preparation of Hydrogel Beads

Kazi Anisur Rahman, Gulsan Ara Sathi, Hiroaki Taketa, Mahmoud Farahat, Masahiro Okada, Yasuhiro Torii, Takuya Matsumoto

3D PRINTING AND ADDITIVE MANUFACTURING 2 ( 1 ) 5 - 11 2015年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

Encapsulation of cells in biocompatible polymers such as naturally derived hydrogels is an important technology that is used for cell therapy and tissue engineering. Herein, we introduce a simple and novel 3D-printed device to prepare hydrogel beads. The device is composed of a cylindrical tube with 1 inlet and 16 outlets. For the hydrogel solution, a low-melting-point agarose solution and sodium alginate are used. At a solution flow rate of 250 mu L/min and a stir speed of 500, 1000, or 1250 rpm, the device produced beads of diameters 50-100, 30-60, and 5-30 mu m, respectively. When the device was run at a flow rate of 500 mu L/min at the above-mentioned stir speeds, the bead diameters were 100-150, 60-90, and 30-60 mu m, respectively. Thus, this device can produce hydrogel beads of different sizes by altering the stir speed or the solution flow rate. In addition, we demonstrated that encapsulated MC3T3-E1 cells can survive and proliferate in the fabricated hydrogel when type I collagen is added to the gel solution. The simplicity of its design would make this device a valuable tool for rapid fabrication of cell encapsulated hydrogels.

DOI： 10.1089/3dp.2014.0023

充填用グラスアイオノマーセメントの初期の歯質接着強さと曲げ特性コーティング材塗布の効果

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 皆木省吾, 長尾紀幸

日本歯科理工学会誌 34 ( 2 ) 81 - 81 2015年3月

Oriented bone formation using biomimetic fibrin hydrogels with three-dimensional patterned bone matrices 査読

Jun-Ichi Sasaki, Takuya Matsumoto, Satoshi Imazato

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 103 ( 2 ) 622 - 627 2015年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY-BLACKWELL

Cortical bone exerts its biological properties through internal orientations of hydroxyapatite. Recently, we succeeded in fabricating three-dimensional (3D) patterns of bone matrices using fibrin gels. We hypothesized that these fibrin gels would induce cortical bone-like tissue in vivo and offer promising prospects for biomimetic materials. The purposes of this study were to assess the characteristics of osteoblastic-induced fibrin gels in vitro and evaluate whether fibrin gels with biomimetic matrices formed cortical bone-like tissue in vivo. When bone marrow stromal cell (BMSC)-containing fibrin gels were uniaxially fixed and cultured, BMSCs became aligned parallel to the fixed direction. The tensile strengths of the gels increased dramatically from 1.5 to 218 kPa after 50 days in culture. Furthermore, Fourier-transform infrared spectroscopy revealed that the fibrin gel constituents changed into those of native bone. Quantitative analyses of specific components demonstrated that the amounts of calcium and osteopontin in the gels increased with prolonged culture. After subcutaneous implantation into immunodeficient mice, cortical bone-like tissues that possessed layered structures were formed. The results indicated that 3D patterned bone matrices induced 3D patterned cortical bone formation in vivo. These biomimetic materials containing 3D patterned bone matrices are promising tools for the field of bone regenerative medicine. (C) 2014 Wiley Periodicals, Inc.

DOI： 10.1002/jbm.a.35212

Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF-β3-Associated Chondrogenesis of Bone Marrow-Derived Mesenchymal Stem Cells for Articular Surface Regeneration. 査読

Hara ES, Ono M, Pham HT, Sonoyama W, Kubota S, Takigawa M, Matsumoto T, Young MF, Olsen BR, Kuboki T

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 30 ( 9 ) 1585 - 1596 2015年

Antagonistic Effects of Insulin and TGF-β3 during Chondrogenic Differentiation of Human BMSCs under a Minimal Amount of Factors. 査読

Hara ES, Ono M, Yoshioka Y, Ueda J, Hazehara Y, Pham HT, Matsumoto T, Kuboki T

Cells, tissues, organs 201 ( 2 ) 88 - 96 2015年

Ultra-structural evaluation of an anodic oxidated titanium dental implant 査読

Akiyoshi Yamagami, Noriyuki Nagaoka, Kumiko Yoshihara, Mariko Nakamura, Hajime Shirai, Takuya Matsumoto, Kazuomi Suzuki, Yasuhiro Yoshida

DENTAL MATERIALS JOURNAL 33 ( 6 ) 828 - 834 2014年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Anodic oxidation is used for the surface treatment of commercial implants to improve their functional properties for clinical success. Here we conducted ultrastructural and chemical investigations into the micro- and nanostructure of the anodic oxide film of a titanium implant. The anodic oxidized layer of a Ti6Al4V alloy implant was examined ultrastructurally by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). They were also analyzed using energy dispersive X-ray spectrometry (EDS) and X-ray photoelectron spectroscopy (XPS). The TEM revealed that the oxide layer of the Ti6Al4V implant prepared through anodic oxidation was separated into two layers. Al and V were not present on the top surface of the anodic oxide. This can be attributed to the biocompatibility of the anodic oxidized Ti6Al4V alloy implant, because the release of harmful metal ions such as Al and V can be suppressed by the biocompatibility.

DOI： 10.4012/dmj.2014-121

ニケイ酸リチウムガラスとレジンセメントの接着シラン処理材の影響

丸尾幸憲, 西川悟郎, 入江正郎, 吉原久美子, 長岡紀幸, 松本卓也, 皆木省吾

接着歯学 32 ( 3 ) 175 - 175 2014年11月

セルフアドヘッシブ・レジンセメントの劣化加速試験後の機械的特性

入江正郎, 田仲持郎, 松本卓也, 長岡紀幸, 丸尾幸憲, 西川悟郎, 皆木省吾, 吉山昌宏

接着歯学 32 ( 3 ) 166 - 166 2014年11月

マウス大腿骨の二次骨化中の組織石灰化に関する定性分析(Qualitative analysis of tissue mineralization during secondary ossification of mouse femur)

ハラ・エミリオ・サトシ, 岡田正弘, 服部高子, 久保田聡, 窪木拓男, 松本卓也

日本バイオマテリアル学会大会予稿集 36回 251 - 251 2014年11月

Comparative Analysis of Mouse-Induced Pluripotent Stem Cells and Mesenchymal Stem Cells During Osteogenic Differentiation In Vitro 査読

Hiroshi Egusa, Hiroki Kayashima, Jiro Miura, Shinya Uraguchi, Fangfang Wang, Hiroko Okawa, Jun-Ichi Sasaki, Makio Saeki, Takuya Matsumoto, Hirofumi Yatani

STEM CELLS AND DEVELOPMENT 23 ( 18 ) 2156 - 2169 2014年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

Induced pluripotent stem cells (iPSCs) can differentiate into mineralizing cells and are, therefore, expected to be useful for bone regenerative medicine; however, the characteristics of iPSC-derived osteogenic cells remain unclear. Here, we provide a direct in vitro comparison of the osteogenic differentiation process in mesenchymal stem cells (MSCs) and iPSCs from adult C57BL/6J mice. After 30 days of culture in osteogenic medium, both MSCs and iPSCs produced robustly mineralized bone nodules that contained abundant calcium phosphate with hydroxyapatite crystal formation. Mineral deposition was significantly higher in iPSC cultures than in MSC cultures. Scanning electron microscopy revealed budding matrix vesicles in early osteogenic iPSCs; subsequently, the vesicles propagated to exhibit robust mineralization without rich fibrous structures. Early osteogenic MSCs showed deposition of many matrix vesicles in abundant collagen fibrils that became solid mineralized structures. Both cell types demonstrated increased expression of osteogenic marker genes, such as runx2, osterix, dlx5, bone sialoprotein (BSP), and osteocalcin, during osteogenesis; however, real-time reverse transcription-polymerase chain reaction array analysis revealed that osteogenesis-related genes encoding mineralization-associated molecules, bone morphogenetic proteins, and extracellular matrix collagens were differentially expressed between iPSCs and MSCs. These data suggest that iPSCs are capable of differentiation into mature osteoblasts whose associated hydroxyapatite has a crystal structure similar to that of MSC-associated hydroxyapatite; however, the transcriptional differences between iPSCs and MSCs could result in differences in the mineral and matrix environments of the bone nodules. Determining the biological mechanisms underlying cell-specific differences in mineralization during in vitro iPSC osteogenesis may facilitate the development of clinically effective engineered bone.

DOI： 10.1089/scd.2013.0344

充填用グラスアイオノマーセメントの硬化初期の歯質接着強さと機械的強さ

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 長岡紀幸

日本歯科理工学会誌 33 ( 5 ) 483 - 483 2014年9月

コンポジットレジン修復の歯質接着強さの経時的推移

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 32 ( 1 ) 8 - 13 2014年4月

記述言語：日本語出版者・発行元：(一社)日本接着歯学会

コンポジットレジンシステム(以後CRSと表記)における初期硬化頃の歯質接着強さの推移を詳細に把握するため、光照射直後から24時間後までのエナメル質および象牙質に対する接着強さを測定した。材料は、市販の9種のCRS[QuiXX+Xeno IV(Dentsply/Caulk)、Filtek P-60+Adper Prompt L-Pop(3M ESPE)、Herculite XRV+OptiBond All-in-One(Kerr)、Tetric N-Ceram+AdheSE One VivaPen(Ivoclar Vivadent)、Solare P+G-Bond(GC)、BEAUTIFIL II+BeautiBond(Shofu)、Metafil AP+Brush & Bond(SunMedical)、Estelite P quick+Tokuyama Bond Force(Tokuyama Dental)、Clearfil Majesty Posterior+Clearfil tri-S Bond(Kuraray Noritake Dental)]を用いた。方法として、エナメル質および象牙質に対するせん断接着強さを、光照射直後、10分間、30分間、1時間、6時間、12時間および24時間37℃蒸留水中に浸漬後、測定した。その結果、エナメル質では、光照射直後の接着強さ(8.0-14.6 MPa)は、24時間後(13.6-21.8 MPa)の51-86%を示し、光照射直後と比較して、10分後では3種のCRSが、6時間後では3種のCRSにおいてそれぞれ有意に接着強さが向上した。象牙質では、光照射直後の接着強さ(7.8-13.3 MPa)は、24時間後(11.0-23.3 MPa)の54-73%を示し、光照射直後の接着強さと比較して、1時間後では1種のCRSが、6時間後では7種のCRSにおいてそれぞれ有意に接着強さが向上した。よって、CRSの歯質接着強さは、光照射直後からほぼ24時間後まで経時的に徐々に向上することが示された。(著者抄録)

二官能メタクリレートの添加が即時重合レジンの重合収縮に与える影響

山本美恵, 丸尾幸憲, 入江正郎, 西川悟郎, 松本卓也, 皆木省吾

日本歯科理工学会誌 33 ( 2 ) 121 - 121 2014年3月

合着用レジン配合型グラスアイオノマーセメントの硬化初期の接着性と曲げ特性

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 長岡紀幸

日本歯科理工学会誌 33 ( 2 ) 153 - 153 2014年3月

miRNA-720 regulates the stem cell phenotype and differentiation of human dental pulp-derived mesenchymal stromal cells 査読

Hara, Emilio Satoshi, Ono, Mitsuaki, Eguchi, Takanori, Hai Thanh Pham, Tajima, Shoji, Calderwood, Stuart K., Matsumoto, Takuya, Kuboki, Takuo, IEEE

2014 International Symposium on Micro-Nanomechatronics and Human Science (Mhs) 1 - 2 2014年

1P176 原子間力顕微鏡によるマウス顎下腺組織の弾性率マッピング測定(12. 細胞生物的課題,ポスター,第52回日本生物物理学会年会(2014年度))

Nakamura Mitsuhiro, Fujii Yuki, Taketa Hiroaki, Matsumoto Takuya, Okajima Takaharu

生物物理 54 ( 1 ) S170 2014年

セルフアドヘッシヴ・レジンセメントのサーマルサイクル負荷後のジルコニアに対する接着強さと曲げ特性

入江正郎, 田仲持郎, 松本卓也, 長岡紀幸, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 31 ( 3 ) 139 - 139 2013年11月

最近のセルフアドヘッシヴ・レジンセメントの硬化初期における接着特性

丸尾幸憲, 西川悟郎, 入江正郎, 長岡紀幸, 松本卓也, 皆木省吾

接着歯学 31 ( 3 ) 138 - 138 2013年11月

ジルコニア表面に対するリン酸モノマー処理の検討

長岡紀幸, 玉田宜之, 入江正郎, 吉原久美子, 吉田靖弘, 西川悟郎, 丸尾幸憲, 松本卓也, 皆木省吾, 早川聡

日本歯科理工学会誌 32 ( 5 ) 354 - 354 2013年9月

合着用レジン添加型グラスアイオノマーセメントの種々の被着体への接着性と曲げ特性サーマルサイクルの影響

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 31 ( 2 ) 45 - 52 2013年8月

記述言語：日本語出版者・発行元：(一社)日本接着歯学会

6種の合着用レジン添加型グラスアイオノマーセメント[RMGICs:RelyX Luting Plus AutomixとVitremer Paste(両者3M ESPE)、Fuji Luting EXとFuji Luting S(両者GC)、SI-R21202とResiGlass Paste(両者松風)]の合着材としての有用性を検討する一環として、歯質、ジルコニア(Lava、3M ESPE)、金合金(GC's Casting Gold Type III M.C.、GC)や金銀パラジウム合金(CastWell M.C.12%Gold、GC)に対するせん断接着強さおよび曲げ特性(強さと弾性率)を硬化直後、1時間水中浸漬後、1日間水中浸漬後およびサーマルサイクル10,000回負荷(TC 10k)後に測定した。その結果、1時間水中浸漬後のRMGICの様々な接着強さは、硬化直後より有意に高く、1日間水中浸漬後の歯質接着強さは1時間水中浸漬後より有意に高かった。TC 10k後では多くのRMGICがそれぞれの測定項目で、大半が有意に低下した。曲げ特性に関しても前述と類似の傾向を示した。(著者抄録)

Application of Cyclic Strain for Accelerated Skeletal Myogenic Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stromal Cells with Cell Alignment 査読

Hiroshi Egusa, Munemasa Kobayashi, Takuya Matsumoto, Jun-Ichi Sasaki, Shinya Uraguchi, Hirofumi Yatani

TISSUE ENGINEERING PART A 19 ( 5-6 ) 770 - 782 2013年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

The fabrication of biomimetic skeletal myocyte constructs continues to present a challenge to functional tissue engineering. The skeletal myogenesis of bone marrow-derived mesenchymal stromal cells (BMSCs) to mimic the native tissue architecture offers great therapeutic promise, but remains particularly difficult. The aim of this study was to examine the possibility of accelerating the skeletal myogenic differentiation of BMSCs with an aligned structure by applying cyclic strain. Mouse BMSCs (mBMSCs) were plated on silicone sheets that were coated with fibronectin and subjected to cyclic 10% uniaxial strain when they reached 80%-90% cell confluency. Cells cultured in a growth medium that were subjected to cyclic strain at a frequency of 0.17 Hz (10 times/min) demonstrated a shift of alignment within 48 h from a completely random orientation to a well-aligned morphology with well-organized actin stress fibers that were parallel to the strain vector. The cyclic strain restricted the motility and proliferation of the aligned mBMSCs in the growth medium, which resulted in tight cellular contact in the cell population. When mBMSCs were subjected to cyclic strain in a myogenic medium, reverse transcription-polymerase chain reaction analysis demonstrated the upregulation of skeletal myogenic marker genes (myogenic factor 5 [Myf5], myogenin, and myogenic regulatory factor 4 [MRF4]), but not smooth muscle marker genes (myocardin and a-smooth muscle actin). In addition, immunocytochemistry showed that the mBMSCs fused to form multinucleated myosin-and myogenin-positive myotubes in the direction of the applied tension within 5 days. These results demonstrate that our simple method of applying of cyclic strain to cells cultured in a myogenic medium greatly accelerates the skeletal myogenic differentiation of mBMSCs with an aligned structure, and they highlight the importance of cellular alignment for creating physiologically relevant environments to study the myogenesis of BMSCs and engineer skeletal muscle.

DOI： 10.1089/ten.tea.2012.0164

Application of Cyclic Strain for Accelerated Skeletal Myogenic Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stromal Cells with Cell Alignment 査読

Hiroshi Egusa, Munemasa Kobayashi, Takuya Matsumoto, Jun-Ichi Sasaki, Shinya Uraguchi, Hirofumi Yatani

TISSUE ENGINEERING PART A 19 ( 5-6 ) 770 - 782 2013年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

The fabrication of biomimetic skeletal myocyte constructs continues to present a challenge to functional tissue engineering. The skeletal myogenesis of bone marrow-derived mesenchymal stromal cells (BMSCs) to mimic the native tissue architecture offers great therapeutic promise, but remains particularly difficult. The aim of this study was to examine the possibility of accelerating the skeletal myogenic differentiation of BMSCs with an aligned structure by applying cyclic strain. Mouse BMSCs (mBMSCs) were plated on silicone sheets that were coated with fibronectin and subjected to cyclic 10% uniaxial strain when they reached 80%-90% cell confluency. Cells cultured in a growth medium that were subjected to cyclic strain at a frequency of 0.17 Hz (10 times/min) demonstrated a shift of alignment within 48 h from a completely random orientation to a well-aligned morphology with well-organized actin stress fibers that were parallel to the strain vector. The cyclic strain restricted the motility and proliferation of the aligned mBMSCs in the growth medium, which resulted in tight cellular contact in the cell population. When mBMSCs were subjected to cyclic strain in a myogenic medium, reverse transcription-polymerase chain reaction analysis demonstrated the upregulation of skeletal myogenic marker genes (myogenic factor 5 [Myf5], myogenin, and myogenic regulatory factor 4 [MRF4]), but not smooth muscle marker genes (myocardin and a-smooth muscle actin). In addition, immunocytochemistry showed that the mBMSCs fused to form multinucleated myosin-and myogenin-positive myotubes in the direction of the applied tension within 5 days. These results demonstrate that our simple method of applying of cyclic strain to cells cultured in a myogenic medium greatly accelerates the skeletal myogenic differentiation of mBMSCs with an aligned structure, and they highlight the importance of cellular alignment for creating physiologically relevant environments to study the myogenesis of BMSCs and engineer skeletal muscle.

DOI： 10.1089/ten.tea.2012.0164

ロカテック処理したジルコニア表面の断面TEM観察

玉田宜之, 長岡紀幸, 武田宏明, 吉原久美子, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 鳥井康弘, 松本卓也, 皆木省吾

日本歯科理工学会誌 32 ( 2 ) 152 - 152 2013年3月

二ケイ酸リチウムガラスセラミックスの接着強さにシランカップリング剤分子中の加水分解基数が及ぼす影響

西川悟郎, 入江正郎, 丸尾幸憲, 山本美恵, 玉田宜之, 前田直人, 長岡紀幸, 松本卓也, 皆木省吾

日本歯科理工学会誌 32 ( 2 ) 154 - 154 2013年3月

Phosphorylated Pullulan Bioadhesive for Regeneration and Reconstruction of Bone and Tooth 査読

Yasuhiro Yoshida, Takumi Okihara, Mariko Nakamura, Takuya Matsumoto

BIOCERAMICS 24 529-530 516 - + 2013年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：TRANS TECH PUBLICATIONS LTD

A biodegradable material that bonds to hard tissues such as bones and teeth is urgently needed for medical and dental applications. However, such materials are not available in today's clinical practice of orthopedics and dentistry. Therefore, we synthesized biodegradable phosphorylated pullulan to develop a biomaterial that combines primary properties such as high biocompatibility, good bonding potential to hard tissue, high strength, biodegradability, and osteoconductivity. The pharmacopoeial polysaccharide pullulan was chemically functionalized with dihydrogen phosphate groups. Phosphorylated pullulan was formed network by adding calcium ion, making the composite less soluble in water. Adhesive force measurement revealed that adhesiveness of the pastes before setting can be controlled through optimization of additives. In addition, histological evaluation revealed that phosphorylated pullulan-based composite possesses high biocompatibility. These results indicate that phosphorylated pullulan can be used as a key material for regeneration and reconstruction of bone and tooth.

DOI： 10.4028/www.scientific.net/KEM.529-530.516

In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO2: HA formation, cellular affinity and organ culture

Sang-Hyun An, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroyuki Miyajima, Ramaswamy Narayanan, Satoshi Imazato, Kyo-Han Kim

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 32 ( 8 ) 2516 - 2522 2012年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Anodization is an easily viable technique useful for producing TiO2 coatings on titanium substrates. Nanocrystalline anodic TiO2 structure was produced on titanium at 20 V using 1 M Na2SO4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO2 was produced on titanium by applying a galvanostatic current density of 70 A/m(2) in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface. (C) 2012 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.msec.2012.07.034

Porous zirconia/hydroxyapatite scaffolds for bone reconstruction 査読

Sang-Hyun An, Takuya Matsumoto, Hiroyuki Miyajima, Atsushi Nakahira, Kyo-Han Kim, Satoshi Imazato

DENTAL MATERIALS 28 ( 12 ) 1221 - 1231 2012年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Objective. Highly porous apatite-based bioceramic scaffolds have been widely investigated as three-dimensional (3D) templates for cell adhesion, proliferation, and differentiation promoting the bone regeneration. Their fragility, however, limits their clinical application especially for a large bone defect.
Methods. To address the hypothesis that using a ZrO2/hydroxyapatite (HAp) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO2/HAp composite scaffolds with different ZrO2/HAp ratios, and evaluated their characteristics. In addition, porous ZrO2/HAp scaffolds containing bone marrow derived stromal cells (BMSCs) were implanted into critical-size bone defects for 6 weeks in order to evaluate the bone tissue reconstruction with this material.
Results. The porosity of a ZrO2/HAp scaffold can be adjusted from 72% to 91%, and the compressive strength of the scaffold increased from 2.5 to 13.8 MPa when the ZrO2 content increased from 50 to 100 wt%. The cell adhesion and proliferation in the ZrO2/HAp scaffold was greatly improved when compared to the scaffold made with ZrO2 alone. Moreover, in vivo study showed that a BMSCs-loaded ZrO2/HAp scaffold provided a suitable 3D environment for BMSC survival and enhanced bone regeneration around the implanted material.
Significance. We thus showed that a porous ZrO2/HAp composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed in the treatment of large bone defects. (C) 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.dental.2012.09.001

In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO2: HA formation, cellular affinity and organ culture 査読

Sang-Hyun An, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroyuki Miyajima, Ramaswamy Narayanan, Satoshi Imazato, Kyo-Han Kim

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 32 ( 8 ) 2516 - 2522 2012年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Anodization is an easily viable technique useful for producing TiO2 coatings on titanium substrates. Nanocrystalline anodic TiO2 structure was produced on titanium at 20 V using 1 M Na2SO4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO2 was produced on titanium by applying a galvanostatic current density of 70 A/m(2) in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface. (C) 2012 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.msec.2012.07.034

合着用レジン添加型グラスアイオノマーセメントの歯質接着性と曲げ特性

入江正郎, 田仲持郎, 松本卓也, 玉田宜之, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 30 ( 4 ) 137 - 144 2012年12月

記述言語：日本語出版者・発行元：(一社)日本接着歯学会

5種の合着用レジン添加型グラスアイオノマーセメント[RMGICs:Fuji Luting EX、Fuji Luting S(ジーシー)、RelyX Luting Plus Automix、Vitremer Paste(スリーエム・エスペ)、ResiGlass Paste(松風)]の歯質接着性と曲げ特性を測定、レジンセメント[SA Luting(クラレノリタケ・デンタル)]と比較した。エナメル質と象牙質に対するせん断接着強さと曲げ強さと曲げ弾性率を、初期硬化、1時間水中浸漬後および1日間水中浸漬後測定した。1時間水中浸漬後のRMGICsとレジンセメントの歯質接着強さは、初期硬化より有意に高く、1日間水中浸漬後の歯質接着強さは1時間水中浸漬後より有意に高かった。曲げ特性に関しては類似の傾向を示したが、レジンセメントはRMGICsと比較して有意に高い値を示した。RMGICsのエナメル質と象牙質に対する接着強さと曲げ特性はそれぞれ有意な正の関係にあった。(著者抄録)

HEMA Inhibits Interfacial Nano-layering of the Functional Monomer MDP

Y. Yoshida, K. Yoshihara, S. Hayakawa, N. Nagaoka, T. Okihara, T. Matsumoto, S. Minagi, A. Osaka, K. Van Landuyt, B. Van Meerbeek

JOURNAL OF DENTAL RESEARCH 91 ( 11 ) 1060 - 1065 2012年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SAGE PUBLICATIONS INC

Previous research showed that the functional monomer 10-methacryloxydecyl dihydrogen phosphate (MDP) ionically bonds to hydroxyapatite (HAp) and forms a nano-layered structure at the interface with HAp-based substrates. Such hydrophobic nano-layering is considered to contribute to the long-term durability of the bond to tooth tissue. However, dental adhesives are complex mixtures usually containing different monomers. This study investigated the effect of the monomer 2-hydroxyethylmethacrylate (HEMA) on the chemical interaction of MDP with HAp by x-ray diffraction (XRD), nuclear magnetic resonance (NMR), and quartz crystal microbalance (QCM). We examined the chemical interaction of 5 experimental MDP solutions with increasing concentrations of HEMA. XRD revealed that addition of HEMA inhibits nano-layering at the interface, while NMR confirmed that MDP remained adsorbed onto the HAp surface. QCM confirmed this adsorption of MDP to HAp, as well as revealed that the demineralization rate of HAp by MDP was reduced by HEMA. It was concluded that even though the adsorption of MDP to HAp was not hindered, addition of HEMA inhibited interfacial nano-layering. Potential consequences with regard to bond durability necessitate further research.

DOI： 10.1177/0022034512460396

新しい多用途型処理材の接着強さからの検討

入江正郎, 田仲持郎, 松本卓也, 玉田宜之, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 30 ( 3 ) 126 - 126 2012年11月

汚染されたジルコニア表面に対するリン酸エステルによるカップリング処理

長岡紀幸, 玉田宜之, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 松本卓也

接着歯学 30 ( 3 ) 114 - 114 2012年11月

ジルコニアに対するリン酸モノマーの吸着特性

玉田宜之, 長岡紀幸, 早川聡, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 松本卓也, 尾坂明義, 皆木省吾

日本歯科理工学会誌 31 ( 5 ) 479 - 479 2012年9月

二官能メタクリレートの添加が即時重合レジンの曲げ特性に与える影響

山本美恵, 丸尾幸憲, 入江正郎, 西川悟郎, 玉田宜之, 松本卓也, 皆木省吾

日本歯科理工学会誌 31 ( 5 ) 452 - 452 2012年9月

即時重合レジンの重合収縮の計測方法に関する研究

丸尾幸憲, 山本美恵, 入江正郎, 西川悟郎, 玉田宜之, 松本卓也, 皆木省吾

日本歯科理工学会誌 31 ( 5 ) 458 - 458 2012年9月

Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis

Kenichi Harimoto, Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Yoh-ichi Tagawa

DENTAL MATERIALS JOURNAL 31 ( 4 ) 674 - 680 2012年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Titanium has an osseointegrative property, while hydroxyapatite has an osteoconductive property. It remains a matter of controversy among researchers whether hydroxyapatite has higher osteoblast compatibility than titanium. Here, we compared the activities between osteoblasts cultured on titanium and those cultured on hydroxyapatite. An osteoblast-like cell line, MC3T3-E1, was cultured on machined titanium, evaporated titanium, and hydroxyapatite disks to compare the affinity of osteoblasts to each of these materials. The adhesion and proliferation of MC3T3-E1 cells were higher on hydroxyapatite disks than on the other disks. Osteoblast differentiation was not affected by the nature of disks investigated, but calcium was more easily deposited on the hydroxyapatite disks. The amount of absorbed serum proteins detected on hydroxyapatite was greater than that on titanium. In conclusion, our results indicate hydroxyapatite is a more suitable material for osteoblast growth than titanium because of its higher absorption of serum proteins.

DOI： 10.4012/dmj.2012-075

X-ray diffraction analysis of three-dimensional self-reinforcing monomer and its chemical interaction with tooth and hydroxyapatite 査読

Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Masao Hanabusa, Takuya Matsumoto, Yasuko Momoi

DENTAL MATERIALS JOURNAL 31 ( 4 ) 697 - 702 2012年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

According to the 'Adhesion-Decalcification' concept, specific functional monomers within dental adhesives can ionically interact with hydroxyapatite (HAp). Some specific functional monomers form monomer-Ca salts due to chemical interactions. The chemical stability of the monomer-Ca salts was thought to contribute to bond durability. In the present study, we analyzed the chemical interaction between an acidic three-dimensional self-reinforcing monomer (3D-SR) of Bond Force and enamel, dentin and HAp, and assessed its chemical stability by thin-film X-ray diffraction (TF-XRD). 3D-SR forms a hydrolysis-resistant Ca-salt on the dentin in a clinical application time period and on enamel and HAp in a longer time period. This suggests that the functional monomer 3D-SR is able to contribute to bond durability.

DOI： 10.4012/dmj.2012-074

Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis 査読

Kenichi Harimoto, Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Yoh-ichi Tagawa

DENTAL MATERIALS JOURNAL 31 ( 4 ) 674 - 680 2012年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Titanium has an osseointegrative property, while hydroxyapatite has an osteoconductive property. It remains a matter of controversy among researchers whether hydroxyapatite has higher osteoblast compatibility than titanium. Here, we compared the activities between osteoblasts cultured on titanium and those cultured on hydroxyapatite. An osteoblast-like cell line, MC3T3-E1, was cultured on machined titanium, evaporated titanium, and hydroxyapatite disks to compare the affinity of osteoblasts to each of these materials. The adhesion and proliferation of MC3T3-E1 cells were higher on hydroxyapatite disks than on the other disks. Osteoblast differentiation was not affected by the nature of disks investigated, but calcium was more easily deposited on the hydroxyapatite disks. The amount of absorbed serum proteins detected on hydroxyapatite was greater than that on titanium. In conclusion, our results indicate hydroxyapatite is a more suitable material for osteoblast growth than titanium because of its higher absorption of serum proteins.

DOI： 10.4012/dmj.2012-075

Surface characterization of alkali- and heat-treated Ti with or without prior acid etching

Sang-Hyun An, Takuya Matsumoto, Hiroyuki Miyajima, Jun-Ichi Sasaki, Ramaswamy Narayanan, Kyo-Han Kim

APPLIED SURFACE SCIENCE 258 ( 10 ) 4377 - 4382 2012年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Titanium and its alloys are used as implant materials in dental and orthopaedic applications. The material affinities to host bone tissue greatly concern with the recovery period and good prognosis. To obtain a material surface having excellent affinity to bone, acid etching prior to alkali- and heat-treatment of Ti was conducted. The surface characteristics of the prepared sample indicated that the roughness as well as the wettability increased by pre-etching. Bone-like apatite was formed on pre-etched, alkali- and heat-treated Ti surface in simulated body fluid (SBF) within 3 days, while it takes 5 days on the solely alkali- and heat-treated surface. Osteoblastic cells showed better compatibility on the per-etched surface compared to the pure Ti surface or alkali- and heat-treated surface. Moreover, the pre-etched surface showed better pull-off tensile adhesion strength against the deposited apatite. Thus, acid etching prior to alkali- and heat-treatment would be a promising method for enhancing the affinity of Ti to host bone tissue. (C) 2012 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.apsusc.2011.12.119

ジルコニア表面のリン酸モノマーによる有機化の検討

玉田宜之, 長岡紀幸, 早川聡, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 松本卓也, 尾坂明義, 皆木省吾

日本歯科理工学会誌 31 ( 2 ) 190 - 190 2012年3月

In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct 査読

Jun-Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Michiya Matsusaki, Akihiro Nishiguchi, Takayoshi Nakano, Mitsuru Akashi, Satoshi Imazato, Hirofumi Yatani

INTEGRATIVE BIOLOGY 4 ( 10 ) 1207 - 1214 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ROYAL SOC CHEMISTRY

Endochondral ossification is one of the essential bone development processes in vertebrates. Although researchers from a variety of fields, including cellular/molecular biology, chemistry, and materials science, have worked to gain a better understanding of the tissue development, integration of findings from these different fields remains a major challenge. An in vitro model system that reproduces endochondral ossification would be a valuable tool for overcoming this problem, because an in vitro standardized model system can be easily accessed by researchers from different fields. Here, we fabricated a large 3D mesenchymal stem cell (MSC) construct with a ball-like morphology, which is termed a cell ball, and cultured it under a hypoxia condition, since hypoxia causes chondrogenic differentiation of MSCs in primordial cartilage, which is crucial for endochondral ossification. Region-specific chondrogenic differentiation of MSCs and mineralization within the cartilage tissue were observed in the cell ball. The precipitated minerals were detected as hydroxyapatite. Consequently, a 3D construct consisting of mineralized tissue surrounded by cartilage tissue was obtained. Moreover, the angiogenic activity of this synthesized tissue changed depending on the chondrogenic phenotype remains in the tissue, which is similar to what happens in the ossification process. Thus, this MSC cell ball system clearly reproduced the initial stage of endochondral ossification in vitro. This system is a promising tool for use as an in vitro model for investigating bone tissue development.

DOI： 10.1039/c2ib20027a

Zirconia-hydroxyapatite composite material with micro porous structure 査読

Takuya Junior Matsumoto, Sang-Hyun An, Takuya Ishimoto, Takayoshi Nakano, Takuya Matsumoto, Satoshi Imazato

DENTAL MATERIALS 27 ( 11 ) E205 - E212 2011年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Objectives. Titanium plates and apatite blocks are commonly used for restoring large osseous defects in dental and orthopedic surgery. However, several cases of allergies against titanium have been recently reported. Also, sintered apatite block does not possess sufficient mechanical strength. In this study, we attempted to fabricate a composite material that has mechanical properties similar to biocortical bone and high bioaffinity by compounding hydroxyapatite (HAp) with the base material zirconia (ZrO2), which possesses high mechanical properties and low toxicity toward living organisms.
Methods. After mixing the raw material powders at several different ZrO2/HAp mixing ratios, the material was compressed in a metal mold (8 mm in diameter) at 5 MPa. Subsequently, it was sintered for 5 h at 1500 degrees C to obtain the ZrO2/HAp composite. The mechanical property and biocompatibility of materials were investigated. Furthermore, osteoconductivity of materials was investigated by animal studies.
Results. A composite material with a minute porous structure was successfully created using ZrO2/HAp powders, having different particle sizes, as the starting material. The material also showed high protein adsorption and a favorable cellular affinity. When the mixing ratio was ZrO2/HAp = 70/30, the strength was equal to cortical bone. Furthermore, in vivo experiments confirmed its high osteoconductivity.
Significance. The composite material had strength similar to biocortical bones with high cell and tissue affinities by compounding ZrO2 and HAp. The ZrO2/HAp composite material having micro porous structure would be a promising bone restorative material. (C) 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.dental.2011.07.009

Zirconia-hydroxyapatite composite material with micro porous structure

Takuya Junior Matsumoto, Sang-Hyun An, Takuya Ishimoto, Takayoshi Nakano, Takuya Matsumoto, Satoshi Imazato

DENTAL MATERIALS 27 ( 11 ) E205 - E212 2011年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Objectives. Titanium plates and apatite blocks are commonly used for restoring large osseous defects in dental and orthopedic surgery. However, several cases of allergies against titanium have been recently reported. Also, sintered apatite block does not possess sufficient mechanical strength. In this study, we attempted to fabricate a composite material that has mechanical properties similar to biocortical bone and high bioaffinity by compounding hydroxyapatite (HAp) with the base material zirconia (ZrO2), which possesses high mechanical properties and low toxicity toward living organisms.
Methods. After mixing the raw material powders at several different ZrO2/HAp mixing ratios, the material was compressed in a metal mold (8 mm in diameter) at 5 MPa. Subsequently, it was sintered for 5 h at 1500 degrees C to obtain the ZrO2/HAp composite. The mechanical property and biocompatibility of materials were investigated. Furthermore, osteoconductivity of materials was investigated by animal studies.
Results. A composite material with a minute porous structure was successfully created using ZrO2/HAp powders, having different particle sizes, as the starting material. The material also showed high protein adsorption and a favorable cellular affinity. When the mixing ratio was ZrO2/HAp = 70/30, the strength was equal to cortical bone. Furthermore, in vivo experiments confirmed its high osteoconductivity.
Significance. The composite material had strength similar to biocortical bones with high cell and tissue affinities by compounding ZrO2 and HAp. The ZrO2/HAp composite material having micro porous structure would be a promising bone restorative material. (C) 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.dental.2011.07.009

Hydrogel-based biomimetic environment for in vitro modulation of branching morphogenesis. 査読

Miyajima H, Matsumoto T, Sakai T, Yamaguchi S, An SH, Abe M, Wakisaka S, Lee KY, Egusa H, Imazato S

Biomaterials 32 ( 28 ) 6754 - 6763 2011年10月

Crystallinity of Anodic TiO(2) Nanotubes and Bioactivity

Sang-Hyun An, Ramaswamy Narayanan, Takuya Matsumoto, Hyo-Jin Lee, Tae-Yub Kwon, Kyo-Han Kim

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 11 ( 6 ) 4910 - 4918 2011年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SCIENTIFIC PUBLISHERS

Anodic TiO(2) nanotubes were produced on titanium at 20 V using 1 M Na(2)SO(4) and 0.5 wt% NaF. Oxidation for 3 hours produced amorphous tubes of diameter 100 nm and thicknesses 2 mu m. Heat-treatments were done for 3 hours at different temperatures. 300 degrees C treatment converted the amorphous coatings to anatase. 550 and 700 degrees C treatments formed dual anatase and rutile; 850 degrees C treatment crystallized to rutile. The treatment at 700 degrees C produced an oxide surface with higher roughness, lower wetting angle and higher coating adhesion. Bioactivity of the as-oxidized and heated coatings were evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the dual oxide structure within 3 days. HA was detected after 7 days in the anatase structure and only after 21 days in the amorphous and rutile structures. In vitro cell culture tests done using mouse osteoblasts indicated that, the 700 degrees C-heated surface showed higher levels of cell activity than the other surfaces. It is concluded that the dual rutile and anatase structure formed by heating the oxide at 700 degrees C is the best of the five surfaces tested.

DOI： 10.1166/jnn.2011.4114

Cell-Based Fabrication of Organic/Inorganic Composite Gel Material 査読

Takuya Matsumoto, Ami Mizuno, Miki Kashiwagi, Shin-suke Yoshida, Jun-ichi Sasaki, Takayoshi Nakano

MATERIALS 4 ( 1 ) 327 - 338 2011年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Biomaterials containing components similar to the native biological tissue would have benefits as an implantable scaffold material. To obtain such biomimetic materials, cells may be great contributors because of their crucial roles in synthetic organics. In addition, the synthesized organics-especially those derived from osteogenic differentiated cells-become a place where mineral crystals nucleate and grow even in vitro. Therefore, to fabricate an organic/inorganic composite material, which is similar to the biological osteoid tissue, bone marrow derived mesenchymal stem cells (BMSCs) were cultured in a 3D fibrin gel in this study. BMSCs secreted bone-related proteins that enhanced the biomineralization within the gel when the cells were cultured with an osteogenic differentiation medium. The compositions of both synthesized matrices and precipitated minerals in the obtained materials altered depending on the cell culture period. The mineral obtained in the 3D gel showed low crystalline hydroxyapatite. The composite materials also showed excellent osteoconductivity with new bone formation when implanted in mice tibiae. Thus, we demonstrated the contributions of cells for fabricating implantable organic/inorganic composite gel materials and a method for controlling the material composition in the gel. This cell-based material fabrication method would be a novel method to fabricate organic/inorganic composite biomimetic materials for bone tissue engineering.

DOI： 10.3390/ma4010327

Modulation of nanotube formation in apatite single crystal via organic molecule incorporation

Matsumoto, T., Uddin, M.H., An, S.H., Arakawa, K., Taguchi, E., Nakahira, A., Okazaki, M.

Materials Chemistry and Physics 128 ( 3 ) 455 - 499 2011年

Gingival Fibroblasts as a Promising Source of Induced Pluripotent Stem Cells 査読

Hiroshi Egusa, Keisuke Okita, Hiroki Kayashima, Guannan Yu, Sho Fukuyasu, Makio Saeki, Takuya Matsumoto, Shinya Yamanaka, Hirofumi Yatani

PLOS ONE 5 ( 9 ) e12743 2010年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PUBLIC LIBRARY SCIENCE

Background: Induced pluripotent stem (iPS) cells efficiently generated from accessible tissues have the potential for clinical applications. Oral gingiva, which is often resected during general dental treatments and treated as biomedical waste, is an easily obtainable tissue, and cells can be isolated from patients with minimal discomfort.
Methodology/Principal Findings: We herein demonstrate iPS cell generation from adult wild-type mouse gingival fibroblasts (GFs) via introduction of four factors (Oct3/4, Sox2, Klf4 and c-Myc; GF-iPS-4F cells) or three factors (the same as GF-iPS-4F cells, but without the c-Myc oncogene; GF-iPS-3F cells) without drug selection. iPS cells were also generated from primary human gingival fibroblasts via four-factor transduction. These cells exhibited the morphology and growth properties of embryonic stem (ES) cells and expressed ES cell marker genes, with a decreased CpG methylation ratio in promoter regions of Nanog and Oct3/4. Additionally, teratoma formation assays showed ES cell-like derivation of cells and tissues representative of all three germ layers. In comparison to mouse GF-iPS-4F cells, GF-iPS-3F cells showed consistently more ES cell-like characteristics in terms of DNA methylation status and gene expression, although the reprogramming process was substantially delayed and the overall efficiency was also reduced. When transplanted into blastocysts, GF-iPS-3F cells gave rise to chimeras and contributed to the development of the germline. Notably, the four-factor reprogramming efficiency of mouse GFs was more than 7-fold higher than that of fibroblasts from tail-tips, possibly because of their high proliferative capacity.
Conclusions/Significance: These results suggest that GFs from the easily obtainable gingival tissues can be readily reprogrammed into iPS cells, thus making them a promising cell source for investigating the basis of cellular reprogramming and pluripotency for future clinical applications. In addition, high-quality iPS cells were generated from mouse GFs without Myc transduction or a specific system for reprogrammed cell selection.

DOI： 10.1371/journal.pone.0012743

Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts 査読

Shinya Nakamura, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroshi Egusa, Kuen Yong Lee, Takayoshi Nakano, Taiji Sohmura, Atsushi Nakahira

TISSUE ENGINEERING PART A 16 ( 8 ) 2467 - 2473 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT INC

Apatite biomaterials have potential not only as cell vehicles for engineering bone tissue but also as regulators of calcium (Ca) concentration in situ for controlling osteoblast functions, for example, osteogenic differentiation and fate management of hematopoietic stem cells (HSCs). To design apatite materials having optimal chemical properties for the latter purpose, more detailed investigations into what effect Ca concentrations have on osteoblast functions is crucial. In this study, osteoblasts were cultured at different Ca concentrations, and the temporal alterations in osteogenic differentiation and HSC niche-related protein (angiopoietin-1, 2 [Ang1, 2]) expression were investigated. The different Ca concentrations (1.8-50 mmol/L) in the cell culture medium had no effect on the proliferation of osteoblasts, but did on the cell morphology. The higher Ca concentrations (<6 mmol/L) enhanced the mineralization as well as Ang1 expression. In addition, Ang1 expression in osteoblasts showed higher correlation with expression of connexin43, the major marker of cell-cell interactions, whereas Ang2 related to integrin beta 1, the major marker of cell-matrix interactions. Thus, the local Ca concentration regulates cell morphology through the cell-cell or cell-matrix interactions, leading to the alteration of Ang1 expression in osteoblasts. Since these changes triggered by Ca are concerned with the osteogenic differentiation or reproduction of HSCs niche microenvironment, the results obtained in this study might be useful for designing apatite materials with optimal chemical properties.

DOI： 10.1089/ten.tea.2009.0337

Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts 査読

Shinya Nakamura, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroshi Egusa, Kuen Yong Lee, Takayoshi Nakano, Taiji Sohmura, Atsushi Nakahira

TISSUE ENGINEERING PART A 16 ( 8 ) 2467 - 2473 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT INC

Apatite biomaterials have potential not only as cell vehicles for engineering bone tissue but also as regulators of calcium (Ca) concentration in situ for controlling osteoblast functions, for example, osteogenic differentiation and fate management of hematopoietic stem cells (HSCs). To design apatite materials having optimal chemical properties for the latter purpose, more detailed investigations into what effect Ca concentrations have on osteoblast functions is crucial. In this study, osteoblasts were cultured at different Ca concentrations, and the temporal alterations in osteogenic differentiation and HSC niche-related protein (angiopoietin-1, 2 [Ang1, 2]) expression were investigated. The different Ca concentrations (1.8-50 mmol/L) in the cell culture medium had no effect on the proliferation of osteoblasts, but did on the cell morphology. The higher Ca concentrations (<6 mmol/L) enhanced the mineralization as well as Ang1 expression. In addition, Ang1 expression in osteoblasts showed higher correlation with expression of connexin43, the major marker of cell-cell interactions, whereas Ang2 related to integrin beta 1, the major marker of cell-matrix interactions. Thus, the local Ca concentration regulates cell morphology through the cell-cell or cell-matrix interactions, leading to the alteration of Ang1 expression in osteoblasts. Since these changes triggered by Ca are concerned with the osteogenic differentiation or reproduction of HSCs niche microenvironment, the results obtained in this study might be useful for designing apatite materials with optimal chemical properties.

DOI： 10.1089/ten.tea.2009.0337

Fabrication of Three-Dimensional Cell Constructs Using Temperature-Responsive Hydrogel 査読

Jun-Ichi Sasaki, Taka-Aki Asoh, Takuya Matsumoto, Hiroshi Egusa, Taiji Sohmura, Eben Alsberg, Mitsuru Akashi, Hirofumi Yatani

TISSUE ENGINEERING PART A 16 ( 8 ) 2497 - 2504 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.

DOI： 10.1089/ten.tea.2009.0523

Fabrication of Three-Dimensional Cell Constructs Using Temperature-Responsive Hydrogel 査読

Jun-Ichi Sasaki, Taka-Aki Asoh, Takuya Matsumoto, Hiroshi Egusa, Taiji Sohmura, Eben Alsberg, Mitsuru Akashi, Hirofumi Yatani

TISSUE ENGINEERING PART A 16 ( 8 ) 2497 - 2504 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MARY ANN LIEBERT, INC

A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.

DOI： 10.1089/ten.tea.2009.0523

Apatite Containing Aspartic Acid for Selective Protein Loading

M. Hafiz Uddin, T. Matsumoto, S. Ishihara, A. Nakahira, M. Okazaki, T. Sohmura

JOURNAL OF DENTAL RESEARCH 89 ( 5 ) 488 - 492 2010年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SAGE PUBLICATIONS INC

Physico-chemical modifications of hydroxyapatite (HAp) materials are considered as pre-requisites for the development of new bioactive carrier materials for drug delivery and tissue engineering applications. Since acidic amino acids have well-documented affinities to both HAp and basic proteins, HAp modified by aspartic acid (Asp, acidic amino acid) might be one of the candidate substrates for a basic protein carrier. Here, we synthesized HAp in the presence of various concentrations of Asp and observed that HAp crystallinity and other physico-chemical properties were effectively modulated. Detailed studies indicated that Asp was not incorporated in the HAp crystal lattice, but rather was trapped in HAp crystals. Protein adsorption studies indicated that the HAp particles modified by Asp had a selective loading capacity for basic protein. Therefore, HAp particles containing Asp might have potential in drug delivery applications, especially as the carrier of basic proteins including bFGF and BMP.

DOI： 10.1177/0022034509357309

Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification

Shiho Ishihara, Takuya Matsumoto, Takamasa Onoki, Mohammad Hafiz Uddin, Taiji Sohmura, Atsushi Nakahira

ACTA BIOMATERIALIA 6 ( 3 ) 830 - 835 2010年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Loading and releasing protein in a controllable way is extremely important for the protein vehicles used in bone tissue engineering. To obtain a suitable carrier material for basic proteins, such as BMP or bFGF, hydroxyapatite particles containing mercaptosuccinic acid (mercaptosuccinic acid (Met), (Mer-HAp)) were synthesized Physicochemical evaluation of Mer-HAp suggested that Mer was contained in HAp particles: it either simply adsorbed onto HAp crystals or was trapped among the HAp crystals. A protein adsorption Study using basic and acidic model proteins indicated that the synthesized Mer-HAp had selective loading properties of the basic protein. The loaded protein was gradually released from Mer-HAp in phosphate buffered saline. The protein release rate was different in each Mer-HAp synthesized with a different concentration of Mer. In addition, the protein release from Mer-HAp showed a similar profile with the Ca dissolution in different pH solutions, indicating that the Mer-HAp dissolution was concerned with the protein release from Mer-HAp Thus, Mer-HAp is a useful candidate for the basic protein carrier because it has properties which enable the loading and releasing of protein in a controllable way. (C) 2009 Acta Materialia Inc Published by Elsevier Ltd. All rights reserved

DOI： 10.1016/j.actbio.2009.10.019

Synthesis of hydroxyapatite containing manganese and its evaluation of biocompatibility.

Fujitani W, Hamada Y, Kawaguchi N, Mori S, Daito K, Uchinaka A, Matsumoto T, Kojima Y, Daito M, Nakano T, Matsuura N

Nano Biomed. 2 ( 1 ) 37 - 46 2010年

記述言語：英語出版者・発行元：ナノ・バイオメディカル学会

Biomaterials made using hydroxyapatite (HAp) and calcium phosphates, which are inorganic components of hard tissues, have been developed to fill bone defects caused by various diseases. A biomaterial of HAp containing collagen, an organic component of bone, has also been developed. However, the physical and biological functions of these bone biomaterials remain inadequate.<br>Focusing on Zinc(Zn) and Manganese(Mn) ions out of the divalent cations involved in the activity of integrins, which are molecules existing in cell membranes, we synthesized three kinds of inorganic biomaterials—pure HAp, Zn-containing HAp (Zn-HAp), and Mn-containing HAp (Mn-HAp)—by a wet process. These three kinds of HAp powders were formed into pellets of 6mm diameter and 1mm thickness and their bio-adaptability was investigated.<br>In vitro experiments using osteoblast-like cells (MC3T3E1) demonstrated that Mn-HAp showed higher cell adhesion potential than pure HAp, indicating that Mn addition improves bioresponses. This may be because Mn ions eluted from Mn-HAp pellets activated integrins, which are closely related to the cell adhesion potential of MC3T3E1 cells to the surface of the pellets.

DOI： 10.11344/nano.2.37.

In vitro engineering of transitional tissue by patterning and functional control of cells in fibrin gel 査読

Jun-Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Takayoshi Nakano, Takuya Ishimoto, Taiji Sohmura, Hirofumi Yatani

SOFT MATTER 6 ( 8 ) 1662 - 1667 2010年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ROYAL SOC CHEMISTRY

Tendon insertions show specific three-dimensional (3D) cell and matrix configurations. To fabricate this transitional tissue in vitro, the development of a cell manipulation technique that can control cell and matrix patterning in the 3D construct is crucial. Bone marrow-derived stromal cells and osteoblasts were respectively cultured in fibrin gel using a custom-made device that can apply uniaxial continuous tensile strain to the gel at different strain rates. Cells in the strained fibrin gel showed a specific orientation, which was parallel to the strain direction, because of the structural change of fibrin fibres within the strained gel. The direction of cell proliferation in the strained gel was also restricted to the same direction. Subsequently, linearly aligned cell sets, similar to the cell patterning of tendon tissue, were obtained in this culture system. Notably, linearly aligned extracellular matrix and mineral patterning, which are crucial for the fabrication of tendon insertions, were also confirmed in the strained fibrin gel. A cell proliferation assay indicated that the strained gel with a higher strain rate enhanced cell proliferation in the gel. In contrast, a Real-Time PCR study indicated that the strained gel with a lower strain rate enhanced cell differentiation in the gel. Thus, our new cell culture system enabled us to fabricate a 3D construct containing patterned cells and matrices in a controllable manner. This would be an effective tool for reproducing tendon insertions in vitro.

DOI： 10.1039/b922418a

A small-molecule approach to bone regenerative medicine in dentistry

Hiroshi Egusa, Makio Saeki, Masanori Doi, Sho Fukuyasu, Takuya Matsumoto, Yoshinori Kamisaki, Hirofumi Yatani

Journal of Oral Biosciences 52 ( 2 ) 107 - 118 2010年

記述言語：英語出版者・発行元：Japanese Association for Oral Biology

The resorption of alveolar bone in periodontitis and/or following toothextraction results in a significant problem in an individual's oral health-related quality of life. Strategies employing growth factor proteins and/orstem cell-based therapy have been successfully used to regenerate alveolarbone augmentationregeneration/however, cost-effectiveness issues illustratethe need for developing alternative strategies. One promising approach is the development of conventional chemical and biological therapeuticsto strongly stimulate endogenous cells to regenerate. Small-molecule compounds can have a variety of biological functions, serving as cell signaling molecules, as tools in molecular biology, and as drugs in medicine. Therefore, the discovery of natural and novel small-molecule synthetic regulators of differentiation and maturation of osteoblasts and/or osteoclasts would acceleratethe development of bone regenerative medicine. Recently,"chemical biology" has started to play an increasingly important role in both drug discovery and elucidating the mechanism of biological phenomena. This review first addresses the current needs and strategies for alveolar bone regeneration, followed by the potential small molecules associated with bone regeneration, with a concluding section on chemical biology-based high throughput screening approaches for identifying small molecules targeting osteoblast/osteoclast differentiation.

DOI： 10.2330/joralbiosci.52.107

Organic/inorganic composite material synthesized with osteoblastic cells 査読

T.Matsumoto, A.Mizuno, M.Okazaki, T.Nakano, T.Sohmura

2009年12月

Mass Fabrication of Small Cell Spheroids by Using Micro-patterned Tissue Culture Plate

Akinari Iwasaki, Takuya Matsumoto, Go Tazaki, Hitoshi Tsuruta, Hiroshi Egusa, Hiroyuki Miyajima, Taiji Sohmura

ADVANCED ENGINEERING MATERIALS 11 ( 10 ) 801 - 804 2009年10月

Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts

Hiroshi Egusa, Yoshitoshi Kaneda, Yoshihiro Akashi, Yoshinosuke Hamada, Takuya Matsumoto, Makio Saeki, Devang K. Thakor, Yasuhiko Tabata, Nariaki Matsuura, Hirofumi Yatani

BIOMATERIALS 30 ( 27 ) 4676 - 4686 2009年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Recently, the binding sequence Ser-Val-Val-Tyr-Gly-Leu-Arg (SVVYGLR) was found adjacent to the RGD sequence in osteopontin, suggesting involvement in osteo-immune cross-talk. The aim of this study was to investigate bioactive functions of a synthetic SVVYGLR peptide in osteoprogenitor cells and osteoclasts, and to examine potential applications in bone regeneration. The SVVYGLR peptide significantly enhanced the adhesion and proliferation of several human mesenchymal cells including bone marrow-derived mesenchymal stem cells, and alpha v beta 3 integrin was involved in cell attachment to the peptide. Additionally, the peptide reduced the number of TRAP-positive multinucleated cells during osteoclastogenesis of RAW264.7 cells and normal murine pre-osteoclasts, and also suppressed NFAT activity and expression of osteoclastogenesis-related mRNAs. When standardized bone defects in rat calvariae were filled with a collagen sponge containing the peptide or PBS (control), the number of TRAP-positive osteoclasts in the grafted sites after 3 weeks was significantly lower in the peptide group. By the 5th week, significantly enhanced resorption of the grafted collagen sponge and new bone formation was observed within and surrounding the sponge in the peptide group. These data suggest that SVVYGLR is an effective bioactive peptide for bone tissue regeneration that promotes attachment and proliferation of osteogenic cells while also suppressing osteoclastogenesis. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.biomaterials.2009.05.032

New concept bioceramics composed of octacalcium phosphate (OCP) and dicarboxylic acid-intercalated OCP via hydrothermal hot-pressing

Shiho Ishihara, Takuya Matsumoto, Takamasa Onoki, Taiji Sohmura, Atsushi Nakahira

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 29 ( 6 ) 1885 - 1888 2009年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Octacalcium phosphate (OCP) and adipic acid-intercalated complexed OCP (Adi-OCP) were synthesized. Moreover, we made ceramic bodies out of them through a hydrothermal hot-pressing (HHP) method. Characteristic features of both the powder and ceramics were investigated by the X-ray diffraction method (XRD). Surface morphology of the ceramics was observed by scanning electron microscopy (SEM). Density, compressive strength and pore size distribution of the ceramics were measured. Crystalline structure of the newly developed OCP ceramics had no phase transformation from the starting materials. Moreover, the newly developed OCP ceramics had good mechanical properties only through the HHP treatment with a temperature as low as 110 degrees C. In order to evaluate bioactivity, the ceramics were immersed in simulating body fluid (SBF). It was predicted that OCP and Adi-OCP had better bioactivity than that of conventional HAp ceramics. (C) 2009 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.msec.2009.02.023

Clinical Evaluation of the Efficacy of Removing Microorganisms to Disinfect Patient-Derived Dental Impressions 査読

Hiroshi Egusa, Takao Watamoto, Takuya Matsumoto, Keiko Abe, Munemasa Kobayashi, Yoshihiro Akashi, Hirofumi Yatani

INTERNATIONAL JOURNAL OF PROSTHODONTICS 21 ( 6 ) 531 - 538 2008年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：QUINTESSENCE PUBLISHING CO INC

Purpose: Disinfection of dental impressions is an indispensable procedure for the control of cross-contaminatior however, there is limited information on the efficacy of disinfection under clinical conditions. The objective of this study was to clinically evaluate the disinfection efficacy of commercially available agents in removing oral pathogens from patient-derived impressions. Materials and Methods: Impressions from 54 patients were divided into groups and either left undisinfected or underwent I of 5 disinfection treatments: ( 1) 2% glutaraldehyde (GA), (2) 1% sodium hypochlorite (SH), (3) 0.25% benzalkonium chloride (BC), (4) 1 ppm ozonated water (OW), or (5) the Hygojet/MD520 system (HJ). An impression culture technique using a brain heart infusion agar medium was used to visualize the microbial contamination on the surface of the impression cultures. The persistent presence of oral pathogens on the impression cultures was examined using selective isolation agar plates. Results: The isolation frequencies of streptococci, staphylococci, Candida, methicillin-resistant Staphylococcus aureus, and Pseudomonas aeruginosa species from undisinfected impressions were 100%, 55.6%, 25.9%. 25.9% and 5.6%. respectively. Disinfection with HJ and BC removed the microorganisms with the greatest efficacy, followed by GA, SH, and OW. Potential bacterial contamination could be detected even after disinfection had been performed. Combined use of BC plus GA or SH removed oral pathogens almost completely from dental impressions, Conclusions: This investigation showed that potential contaminants are still present, even after general disinfection procedures. Therefore. either HJ or the combined use of BC with GA or SH is recommended for clinical and laboratory use. Int J Prosthodont 200821,531-538.

Three-dimensional cell and tissue patterning in a strained fibrin gel system.

Matsumoto T, Sasaki J, Alsberg E, Egusa H, Yatani H, Sohmura T

PLoS ONE. 2 e1211 2008年

Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold bioniaterials 査読

Yoshinosuke Hamada, Hiroshi Egusa, Yoshitoshi Kaneda, Isao Hirata, Naomasa Kawaguchi, Takafumi Hirao, Takuya Matsumoto, Makiko Yao, Kiyoshi Daito, Mayuka Suzuki

DENTAL MATERIALS JOURNAL 26 ( 4 ) 487 - 492 2007年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

We have previously reported that an osteopontin -derived SVVYGLR peptide exhibited potent angiogenic activity in vitro and in vivo. In the present study, the focus points were on the in vitro effect of SVVYGLR on bone marrow stromal cell proliferation, as well as its in vivo effect on bone tissue formation when grafts made Of CO3 Ap-collagen sponge-as a scaffold biornaterial containing the SVVYGLR motif - were implanted. SVVYGLR peptide promoted bone marrow stromal cell proliferation. When a CO,Ap-collagen sponge containing SVVYGLR peptide was implanted as a graft into a tissue defect created in rat tibia, the migration of numerous vascular endothelial cells - as well as prominent angiogenesis - inside the graft could be detected after one week. These results thus suggested that our scaffold biomaterials including the peptide could be useful for bone tissue regeneration.

Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold bioniaterials 査読

Yoshinosuke Hamada, Hiroshi Egusa, Yoshitoshi Kaneda, Isao Hirata, Naomasa Kawaguchi, Takafumi Hirao, Takuya Matsumoto, Makiko Yao, Kiyoshi Daito, Mayuka Suzuki

DENTAL MATERIALS JOURNAL 26 ( 4 ) 487 - 492 2007年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

We have previously reported that an osteopontin -derived SVVYGLR peptide exhibited potent angiogenic activity in vitro and in vivo. In the present study, the focus points were on the in vitro effect of SVVYGLR on bone marrow stromal cell proliferation, as well as its in vivo effect on bone tissue formation when grafts made Of CO3 Ap-collagen sponge-as a scaffold biornaterial containing the SVVYGLR motif - were implanted. SVVYGLR peptide promoted bone marrow stromal cell proliferation. When a CO,Ap-collagen sponge containing SVVYGLR peptide was implanted as a graft into a tissue defect created in rat tibia, the migration of numerous vascular endothelial cells - as well as prominent angiogenesis - inside the graft could be detected after one week. These results thus suggested that our scaffold biomaterials including the peptide could be useful for bone tissue regeneration.

DOI： 10.4012/dmj.26.487

Different behavior of implanted hydroxyapatite depending on morphology, size and crystallinity 査読

Takuya Matsumoto, Ken-ichi Tamine, Ryosuke Kagawa, Yoshinosuke Hamada, Masayuki Okazaki, Junzo Takahashi

JOURNAL OF THE CERAMIC SOCIETY OF JAPAN 114 ( 1333 ) 760 - 762 2006年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CERAMIC SOC JAPAN-NIPPON SERAMIKKUSU KYOKAI

Hydroxyapatite (HAp) materials with different morphology, size and crystallinity were prepared and were implanted into a male rat tibia. Each of the HAp materials showed a high biocompatiblity and osteoconductivity. The morphology and size of the implanted HAp affected the intrusion of bone marrow derived cells among HAp materials, and this cell intrusion was important for regulating the newly formed bone mass. High crystalline sintered HAp was stable and remained in its initial shape for long periods. However, low crystalline HAp was absorbed and disappeared during bone remodeling. This study indicates that choosing suitable HAp materials in morphology, size and crystallinity is important for a treatment plan (e.g. reconstruction or regeneration; large region or small region etc.) and for achieving a desired prognosis.

DOI： 10.2109/jcersj.114.760

Different behavior of implanted hydroxyapatite depending on morphology, size and crystallinity

Takuya Matsumoto, Ken-ichi Tamine, Ryosuke Kagawa, Yoshinosuke Hamada, Masayuki Okazaki, Junzo Takahashi

JOURNAL OF THE CERAMIC SOCIETY OF JAPAN 114 ( 1333 ) 760 - 762 2006年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CERAMIC SOC JAPAN-NIPPON SERAMIKKUSU KYOKAI

Hydroxyapatite (HAp) materials with different morphology, size and crystallinity were prepared and were implanted into a male rat tibia. Each of the HAp materials showed a high biocompatiblity and osteoconductivity. The morphology and size of the implanted HAp affected the intrusion of bone marrow derived cells among HAp materials, and this cell intrusion was important for regulating the newly formed bone mass. High crystalline sintered HAp was stable and remained in its initial shape for long periods. However, low crystalline HAp was absorbed and disappeared during bone remodeling. This study indicates that choosing suitable HAp materials in morphology, size and crystallinity is important for a treatment plan (e.g. reconstruction or regeneration; large region or small region etc.) and for achieving a desired prognosis.

Role of acidic amino acid for regulating hydroxyapatite crystal growth 査読

Takuya Matsumoto, Masayuki Okazaki, Masahiro Inoue, Jun-Ichi Sasaki, Yoshinosuke Hamada, Junzo Takahashi

DENTAL MATERIALS JOURNAL 25 ( 2 ) 360 - 364 2006年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Non-collagenous proteins in hard tissue matrix are thought to play a pivotal role in regulating the crystal growth of hydroxyapatite (HAp). As most non-collagenous proteins are acidic proteins containing acidic amino acid-rich sequences, we examined the growth of HAp crystals from HAp seed crystals in the presence/absence of acidic amino acid. New HAp formation generally started from the P-surface of HAp. However, in the presence of acidic amino acid, new HAp formation was observed on both P-surface and C-surface of HAp seed crystals. Furthermore, newly formed HAp showed specific orientation along the long-axis direction of HAp seed crystals. In terms of crystallinity, HAp formed in the presence of acidic amino acid showed low crystallinity. These results suggested that, in biomineralization, the adsorbed or free state of acidic amino acid would influence crystal formation and orientation as follows: 1) If free in solution, acidic amino acid would inhibit HAp crystal growth; 2) If adsorbed or immobilized on matrix, acidic amino acid would become HAp nucleation site and regulate the orientation of HAp crystals.

DOI： 10.4012/dmj.25.360

Cell instructive polymers 査読

Takuya Matsumoto, David J. Mooney

TISSUE ENGINEERING I: SCAFFOLD SYSTEMS FOR TISSUE ENGINEERING 102 113 - 137 2006年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER-VERLAG BERLIN

Polymeric materials used in tissue engineering were initially used solely as delivery vehicles for transplanting cells. However, these materials are currently designed to actively regulate the resultant tissue structure and function. This control is achieved through spatial and temporal regulation of various cues (e.g., adhesion ligands, growth factors) provided to interacting cells from the material. These polymeric materials that control cell function and tissue formation are termed cell instructive polymers, and recent trends in their design are outlined in this chapter.

DOI： 10.1007/b137207

Different behavior of implanted hydroxyapatite depending on morphology, size and crystallinity 査読

Takuya Matsumoto, Ken-Ichi Tamine, Ryosuke Kagawa, Yoshinosuke Hamada, Masayuki Okazaki, Junzo Takahashi

Journal of the Ceramic Society of Japan 114 ( 1333 ) 760 - 762 2006年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Ceramic Society of Japan

Hydroxyapatite (HAp) materials with different morphology, size and crystallinity were prepared and were implanted into a male rat tibia. Each of the HAp materials showed a high biocompatiblity and osteoconductivity. The morphology and size of the implanted HAp affected the intrusion of bone marrow derived cells among HAp materials, and this cell intrusion was important for regulating the newly formed bone mass. High crystalline sintered HAp was stable and remained in its initial shape for long periods. However, low crystalline HAp was absorbed and disappeared during bone remodeling. This study indicates that choosing suitable HAp materials in morphology, size and crystallinity is important for a treatment plan (e.g. reconstruction or regeneration
large region or small region etc.) and for achieving a desired prognosis.

DOI： 10.2109/jcersj.114.760

Advantages of TOF-SIMS analysis of hydroxyapatite and fluorapatite in comparison with XRD, HR-TEM and FT-IR

M Okazaki, Hirata, I, T Matsumoto, J Takahashi

DENTAL MATERIALS JOURNAL 24 ( 4 ) 508 - 514 2005年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

The chemical analysis of hydroxyapatite and fluorapatite was carried out using time-of-flight secondary ion mass spectrometry (TOF-SEAS). Hydroxyapatite and fluorapatite were synthesized at 80 +/- 1 degrees C and pH 7.4 +/- 0.2. Fluorapatite was better crystallized, with its (300) reflection shifted to a slightly higher angle. High-resolution transmission electron microscopy clearly revealed a typical, regular hexagonal cross section perpendicular to the c-axis for fluorapatite and a flattened hexagonal cross section for hydroxyapatite. FT-IR spectra of fluorapatite confirmed the absence of OH absorption peak - which was seen in hydroxyapatite at about 3570 cm(-1). TOF-SIMS mass spectra showed a peak at 40 amu due to calcium. In addition, a peak at 19 amu due to fluorine could be clearly seen, although the intensities Of PO, PO2, and PO3 were very low. It was confirmed that TOF-SIMS clearly showed the differences between positive and negative mass spectra of hydroxyapatite and fluorapatite, especially for F-. We concluded that TOF-SIMS exhibited distinct advantages compared with other methods of analysis.

Fabrication of Al2O3/BN nanocomposites by chemical processing and their mechanical properties

T Kusunose, YH Kim, T Sekino, T Matsumoto, N Tanaka, T Nakayama, K Niihara

JOURNAL OF MATERIALS RESEARCH 20 ( 1 ) 183 - 190 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MATERIALS RESEARCH SOCIETY

Al2O3/BN nanocomposites were fabricated through a novel chemical route involving hot-pressing of alpha-Al2O3 powders covered partly with turbostratic BN (t-BN). The nano-sized hexagonal BN (h-BN) particles were found to be homogeneously dispersed within the Al2O3 grains as well as at grain boundaries, which is indicative of nanocomposite structures. Thus, the present nanocomposites exhibited the unique properties of high strength and low Young's modulus associated with nanocomposites. This paper discusses in detail the synthesis process and microstructural features of these materials.

DOI： 10.1557/JMR.2005.0010

Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo 査読

Y Hamada, K Yuki, M Okazaki, W Fujitani, T Matsumoto, MK Hashida, K Harutsugu, K Nokihara, M Daito, N Matsuura, J Takahashi

DENTAL MATERIALS JOURNAL 23 ( 4 ) 650 - 655 2004年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.

Angiogenic activity of osteopontin-derived peptide SVVYGLR 査読

Y Hamada, K Nokihara, M Okazaki, W Fujitani, T Matsumoto, M Matsuo, Y Umakoshi, J Takahashi, N Matsuura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 310 ( 1 ) 153 - 157 2003年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE

Angiogenesis plays an important role in various pathological conditions as well as some physiological processes. Although a number of soluble angiogenic factors have been reported, extracellular matrix also has crucial effect on angiogenesis through interaction with endothelial cells. Since recent reports showed osteopontin had some angiogenic activity, the effect of the SVVYGLR peptide, novel binding motif in osteopontin molecule, on angiogenesis was examined in this study. Synthetic peptide SVVYGLR did not have proliferative effect on endothelial cells but adhesion and migration activity to endothelial cells. Furthermore, SVVYGLR had as potent activity for tube formation in three-dimensional collagen gel as vascular endothelial growth factor which is known to be the strongest angiogenic factor. Electron microscopical analysis showed a number of microvilli on the endothelial luminar surface and tight junction formation in the luminar intercellular border between endothelial cells, indicating SVVYGLR induced cell porarity and differentiation of endothelial cells. This small peptide might be expected to stimulate angiogenesis to improve some ischemic conditions in the future because of some advantages due to smaller molecular weight. (C) 2003 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2003.09.001

Control of pore size in L-lactide/epsilon-caprolactone copolymer foams for tissue regeneration by the freeze-drying method 査読

H Nakao, SH Hyon, S Tsutsumi, T Matsumoto, J Takahashi

DENTAL MATERIALS JOURNAL 22 ( 3 ) 262 - 271 2003年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

In the regeneration,and repair of missing tissues, synthetic polymer scaffolds need many pores to involve cells and to supply cells with nutrients. The control of the pore size of biodegradable L-lactide/epsilon-caprolactone copolymer foams was studied by changing the polymer concentration and the cooling temperature in the freeze-drying method. The mixtures of polymer and 1, 4-dioxane solution were poured into an 18-8 stainless steel Petri dish and frozen. The pore size of a polymer foam tends to increase from the bottom towards the top of a Petri dish. The pore size decreased to one-half with increasing polymer concentration (1 to 10 wt%). The mean pore size in foams of 8% polymer concentration decreased from 100mum to 20mum as cooling temperature was lowered. This suggests that the,higher cooling rate due to lower cooling temperature can produce smaller ice-crystals and result in smaller pores.

Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts

M Taira, H Nakao, T Matsumoto, J Takahashi

INTERNATIONAL JOURNAL OF PROSTHODONTICS 13 ( 4 ) 311 - 315 2000年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：QUINTESSENCE PUBL CO INC

Purpose: The objective of this study was to examine the cytotoxic effect of methyl methacrylate (MMA) monomer on 4 mammalian fibroblasts. Materials and Methods: Four cells-C3H10T1/2, L929, Balb/3T3 clone A31, and MC3T3-E1-were incubated for 6 days in 24-well microplates filled with 1 mL of Dulbecco's Modified Eagle medium containing MMA monomer that ranged from 0 to 200 mM/L. Cytotoxicity was evaluated by direct cell number count. Results: L929 possessed the largest TC,, value (the amount of MMA that caused 50% cell death) (34 mM/L), and Balb/3T3 clone A31 had the least value (1 mM/L); C3H10T1/2 and MC3T3-E1 had intermediate values (25 mM/L and 16 mM/L, respectively). Conclusion: This study showed that the MMA monomer had dose-dependent cytotoxicity, which Varied among the cells tested.

Inhibiting action of carbohydrates on the growth of fluorapatite crystals

T Matsumoto, M Okazaki, M Taira, J Takahashi

CARIES RESEARCH 34 ( 1 ) 26 - 32 2000年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：KARGER

Hydroxyapatite (HAp) and fluorapatite (FAp) were synthesized in the presence of carbohydrates (glucose, fructose, galactose and sucrose), X-ray diffraction analysis showed that the crystallinity of two FAp groups that were synthesized in the presence of glucose or sucrose decreased with the increase in carbohydrate content, In SEM photographs we found that the size of their crystals was small and the shape changed. However, FAp groups that were snythesized in the presence of fructose or galactose showed little change in their crystallinity and shape, Four carbohydrates had some influence on size, but not on shape or crystallinity when synthesizing HAp. These results suggest that glucose and/or sucrose has a chemical affinity to FAp and inhibits the growth of FAp crystals, Copyright (C) 2000 S. Karger AG, Basel.

Organ-derived dependence of biomineralization in type I collagen gel 査読

T Matsumoto, M Okazaki, M Inoue, Y Hamada, M Taira, J Takahashi

BIOCERAMICS 192-1 709 - 712 2000年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TRANS TECH PUBLICATIONS LTD

The number and the structure of cross-links in type I collagen are different from each organ, therefore, orientation and diameter of type I collagen fibers are also different from each organ. When type I collagen is used as biomaterials for hard tissue regeneration, it can be considered that the characteristics and the quantity of newly created minerals in type I collagen are different depending on organs-derived. In this study, calcium phosphate were synthesized in type I collagen gels derived from pig skin or tendon and bovine bone, and the characteristics of obtained minerals were investigated. It was suggested that all the newly created products were octacalcium phosphate (OCP) in X-ray diffraction analysis. Furthermore, X-ray diffraction analysis showed that minerals obtained in bone collagen gel were significantly high in crystallinity compared with minerals obtained in skin and tendon collagen gel. However, the quantity of the newly created minerals in bone collagen decreased significantly compared with minerals obtained in skin and tendon collagen. Collectively these results suggest that the characteristics and quantity of minerals synthesized in type I collagen are depending on organ of origin.

Functionally graded fluoridated apatites

M Okazaki, Y Miake, H Tohda, T Yanagisawa, T Matsumoto, J Takahashi

BIOMATERIALS 20 ( 15 ) 1421 - 1426 1999年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Fluoridated hydroxyapatite was synthesized at 80 +/- 1 degrees C and pH 7.4 +/- 0.2 using a gradient fluoride supply system. X-ray diffraction analysis showed a typically apatitic pattern, although the (3 0 0) reflection was broader than that of homogeneous fluorapatite. Scanning electron micrographic observation indicated that the apatite was composed of rod-like crystals similarly to fluorapatite. High-resolution transmission electron microscopy showed electron damage in the core of the crystal. When the apatite pellet was prepared, electron spectroscopy for chemical analysis showed a negative gradient of fluoride concentration with depth in the crystals. The apparent solubility in 0.5 mol/l acetate buffer solution (37 degrees C and pH 4.0) was 9.16 +/- 0.39 mmol/l, much less than that of homogeneous hydroxyapatite 32.3 +/- 1.9 mmol/l, and less than that of heterogeneous two-layer fluoridated apatite with an outer fluoride-rich layer 12.5 +/- 0.6 mmol/l, which was synthesized previously by supplying fluoride during the latter half of the experimental period. These results suggest that graded fluoridated apatite may be formed by this process and have higher acid resistance than two-layer fluoridated apatite. (C) 1999 Elsevier Science Ltd. All rights reserved.

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