研究者詳細 - 松本　卓也

Fabrication of Artificial Food Bolus for Evaluation of Swallowing 査読

Miyu Hosotsubo, Tetsuro Magota, Masahiko Egusa, Takuya Miyawaki, Takuya Matsumoto

PLOS ONE 11 ( 12 ) e0168378 2016年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PUBLIC LIBRARY SCIENCE

Simple and easy methods to evaluate swallowing are required because of the recently increased need of rehabilitation for dysphagia. "Artificial food bolus", but not "artificial food", would be a valuable tool for swallowing evaluation without considering the mastication effect which is altered according to the individual's oral condition. Thus, this study was carried out to fabricate artificial bolus resembling natural food bolus. The mechanical property and the volume change of food bolus in normal people were firstly investigated. Thirty healthy adults without dysphagia were selected and asked to chew four sample foods (rice cake, peanut, burdock, and gummy candy). The results indicated that Young's modulus of bolus before swallowing was below 150 kPa. The bolus volume before swallowing was below 400 mm(3). In addition, the saliva component ratio of each bolus was approximately 30wt%, and the average saliva viscosity of research participants was approximately 10 mPa.s. Based on the obtained data, artificial food bolus was designed and fabricated by using alginate hydrogel as a visco-elastic material and gelatin solution as a viscotic material with a ratio of 7: 3 based on weight. Consequently, the swallowing time of fabricated artificial food bolus was measured among the same participants. The results indicated the participants swallowed fabricated food bolus with similar manner reflecting their mechanical property and volume. Thus, this artificial food bolus would be a promising tool for evaluation of swallowing.

DOI： 10.1371/journal.pone.0168378

最近の合着用セメント象牙質とCAD/CAM用レジンブロックに対する接着強さ

入江正郎, 飯田祥与, 丸尾幸憲, 西川悟郎, 皆木省吾, 長岡紀幸, 吉原久美子, 松本卓也

接着歯学 34 ( 4 ) 141 - 149 2016年12月

記述言語：日本語出版者・発行元：(一社)日本接着歯学会

6種の接着システム[3種の処理材併用のレジンセメント;RelyX Ultimate(3M)、Resicem(Shofu)、ESTECEM(Tokuyama Dental)、2種の処理材を併用しないレジンセメント;G-Cem Cerasmart(GC)、SA Luting Plus(Kuraray Noritake Dental)、および1種のレジン配合型グラスアイオノマーセメント;Resiglass(Shofu)]を使用して硬化直後と1日後の象牙質に対する接着強さを検討、次に6種のCAD/CAM冠用レジンブロック[Lava Ultimate(3M)、Cerasmart(GC)、Shofu HC Block(Shofu)、ESTELITE BLOCK(Tokuyama Dental)、KZR-CAD HR(Yamakin)、KATANA Avencia (Kuraray Noritake Dental)]に対して1日後の被着面のサンドブラスト処理有無の影響も接着強さから検討した。その結果、象牙質に対する接着強さは、いずれのセメントも1日後の方が、硬化直後と比較して有意に高い値を示した(t-Test、p<0.05)。1日後のCAD/CAM冠用レジンブロックに対する接着強さは、4種の接着システム(Lava UltimateにScotchbond Universal Adhesive/RelyX Ultimateで接着させた場合、CerasmartにCeramic Primer II/G-Cem Cerasmartで接着させた場合、ESTELITE BLOCKにUNIVERSAL PRIMER/ESTECEMで接着させた場合、KZR-CAD HRにScotchbond Universal Adhesive/RelyX Ultimateで接着させた場合)にサンドブラスト処理することで有意に高い値を示した(t-Test、p<0.05)。しかし、それ以外の3種の接着システム(Shofu HC BlockにPorcelain Primer/Resicemで接着させた場合、Shofu HC BlockにResiglassで接着させた場合、KATANA AvenciaにClearfil Ceramic Primer/SA Luting Plusで接着させた場合)はサンドブラスト処理しても有意に高い値を示さなかった(t-Test、p>0.05)。(著者抄録)

プライマーの併用が可能な新規セルフアドヒーシブ・レジンセメントの歯質接着性と曲げ特性

西川悟郎, 飯田祥与, 丸尾幸憲, 入江正郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 34 ( 3 ) 113 - 113 2016年11月

新規動揺歯固定材の歯質接着性と曲げ特性

入江正郎, 飯田祥与, 丸尾幸憲, 西川悟郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 34 ( 3 ) 117 - 117 2016年11月

Adsorption and desorption behaviors of cetylpyridinium chloride on hydroxyapatite nanoparticles with different morphologies 査読

Masahiro Okada, Daisuke Hiramatsu, Takumi Okihara, Takuya Matsumoto

DENTAL MATERIALS JOURNAL 35 ( 4 ) 651 - 658 2016年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

Application of hydroxyapatite (HAp) nanoparticles to repair damaged enamel has attracted recent attention. In this study, HAp nanoparticles with various morphologies (spherical, short-rod, long-rod and fiber morphologies) were synthesized via chemical precipitation methods without the addition of template molecules, and the adsorption/desorption behaviors of a cationic antibacterial agent, cetylpyridinium chloride (CPC), on the HAp nanoparticles were evaluated. The adsorption of CPC on each HAp nanoparticle showed Langmuir-type adsorption, and the short-rod/long-rod HAp nanoparticles showed thermodynamically more stable adsorption of CPC than that with the spherical/fiber HAp nanoparticles. The desorption rate of CPC from the short-rod/long-rod HAp nanoparticles was slower than that of the spherical/fiber HAp nanoparticles. The HAp nanoparticles with different CPC release profiles presented here have potential applications as nanoparticulate enamel repair agents with antibacterial properties.

DOI： 10.4012/dmj.2015-420

Highly dynamic self-organizing SMG morphogenesis in 3D culture 査読

Mahmoud Farahat, Gulsan Ara Sathi, Hiroaki Taketa, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 1 - 3 2016年3月

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：Institute of Electrical and Electronics Engineers Inc.

Biological tissues have specific cell patterns and well-organized structures which result from various organogenesis processes. Among which is branching morphogenesis which is the key developmental processes for many glandular tissues including lungs, kidney, salivary gland and liver. Many approaches were suggested to regenerate salivary gland tissue in vitro. Here, we developed a 3D culture technique to study and recapitulate the dynamic forces generated during SMG organogenesis by utilizing time lapse live imaging to monitor the cellular migration and reorganization process of both epithelium and mesenchyme cells.

DOI： 10.1109/MHS.2015.7438259

Scopus

Functional peptide KP24 enhances submandibular gland tissue growth in vitro

Atsushi Ikeda, Hiroaki Taketa, Gulsan Ara Sathi, Yoshiaki Hirano, Seiji Iida, Takuya Matsumoto

REGENERATIVE THERAPY 3 108 - 113 2016年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Introduction: Salivary gland hypofunction, also known as xerostomia, occurs as a result of radiotherapy for head and neck cancer, autoimmune diseases, or aging. Xerostomia leads to oral health problems and thus affects the quality of life. Biological salivary gland tissue generated in vitro would provide an alternative mode of treatment for this disease.
Methods: To develop a novel method for modulating salivary gland tissue growth in vitro, we prepared a KP24 peptide-immobilized hydrogel sheet, wherein the peptide comprised repeating proline and lysine sequences, and evaluated the effect of this peptide on salivary gland tissue growth.
Results: We found that the KP24 peptide has the potential to enhance glandular tissue growth in vitro. This enhancement is associated with neurite outgrowth and increasing neural innervation.
Conclusion: KP24 peptide modified material would be a promising material for the modulation of salivary gland tissue growth in vitro. (C) 2016, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.

DOI： 10.1016/j.reth.2016.02.006

Various Effects of Sandblasting of Dental Restorative Materials 査読

Goro Nishigawa, Yukinori Maruo, Masao Irie, Naoto Maeda, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Shogo Minagi

PLOS ONE 11 ( 1 ) e0147077 2016年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PUBLIC LIBRARY SCIENCE

Background
Sandblasting particles which remain on the surfaces of dental restorations are removed prior to cementation. It is probable that adhesive strength between luting material and sandblasting particle remnants might exceed that with restorative material. If that being the case, blasting particles adhere to sandblasted material surface could be instrumental to increasing adhesive strength like underlying bonding mechanism between luting material and silanized particles of tribochemical silica coating-treated surface. We hypothesize that ultrasonic cleaning of bonding surfaces, which were pretreated with sandblasting, may affect adhesive strength of a resin luting material to dental restorative materials.
Methods
We therefore observed adhesive strength of resin luting material to aluminum oxide was greater than those to zirconia ceramic and cobalt-chromium alloy beforehand. To measure the shear bond strengths of resin luting material to zirconia ceramic and cobalt-chromium alloy, forty specimens of each restorative material were prepared. Bonding surfaces were polished with silicon abrasive paper and then treated with sandblasting. For each restorative material, 40 sandblasted specimens were equally divided into two groups: ultrasonic cleaning (USC) group and non-ultrasonic cleaning (NUSC) group. After resin luting material was polymerized on bonding surface, shear test was performed to evaluate effect of ultrasonic cleaning of bonding surfaces pretreated with sandblasting on bond strength.
Results
For both zirconia ceramic and cobalt-chromium alloy, NUSC group showed significantly higher shear bond strength than USC group.
Conclusions
Ultrasonic cleaning of dental restorations after sandblasting should be avoided to retain improved bonding between these materials.

DOI： 10.1371/journal.pone.0147077

Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells 査読

Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

STEM CELLS INTERNATIONAL 2016 6240794 2016年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：HINDAWI LTD

Three-dimensional (3D) cell constructs are expected to provide osteoinductive materials to develop cell-based therapies for bone regeneration. The proliferation and spontaneous aggregation capability of induced pluripotent stemcells (iPSCs) thus prompted us to fabricate a scaffold-free iPSC construct as a transplantation vehicle. Embryoid bodies of mouse gingival fibroblast-derived iPSCs (GF-iPSCs) were seeded in a cell chamber with a round-bottom well made of a thermoresponsive hydrogel. Collected ball-like cell constructs were cultured in osteogenic induction medium for 30 days with gentle shaking, resulting in significant upregulation of osteogenic marker genes. The constructs consisted of an inner region of unstructured cell mass and an outer osseous tissue region that was surrounded by osteoblast progenitor-like cells. The outer osseous tissue was robustly calcified with elemental calcium and phosphorous as well as hydroxyapatite. Subcutaneous transplantation of the GF-iPSC constructs into immunodeficient mice contributed to extensive ectopic bone formation surrounded by teratoma tissue. These results suggest that mouse GF-iPSCs could facilitate the fabrication of osteoinductive scaffold-free 3D cell constructs, in which the calcified regions and surrounding osteoblasts may function as scaffolds and drivers of osteoinduction, respectively. With incorporation of technologies to inhibit teratoma formation, this system could provide a promising strategy for bone regenerative therapies.

DOI： 10.1155/2016/6240794

Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells 査読

Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

Stem Cells International 2016 2016年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Hindawi Limited

Three-dimensional (3D) cell constructs are expected to provide osteoinductive materials to develop cell-based therapies for bone regeneration. The proliferation and spontaneous aggregation capability of induced pluripotent stem cells (iPSCs) thus prompted us to fabricate a scaffold-free iPSC construct as a transplantation vehicle. Embryoid bodies of mouse gingival fibroblast-derived iPSCs (GF-iPSCs) were seeded in a cell chamber with a round-bottom well made of a thermoresponsive hydrogel. Collected ball-like cell constructs were cultured in osteogenic induction medium for 30 days with gentle shaking, resulting in significant upregulation of osteogenic marker genes. The constructs consisted of an inner region of unstructured cell mass and an outer osseous tissue region that was surrounded by osteoblast progenitor-like cells. The outer osseous tissue was robustly calcified with elemental calcium and phosphorous as well as hydroxyapatite. Subcutaneous transplantation of the GF-iPSC constructs into immunodeficient mice contributed to extensive ectopic bone formation surrounded by teratoma tissue. These results suggest that mouse GF-iPSCs could facilitate the fabrication of osteoinductive scaffold-free 3D cell constructs, in which the calcified regions and surrounding osteoblasts may function as scaffolds and drivers of osteoinduction, respectively. With incorporation of technologies to inhibit teratoma formation, this system could provide a promising strategy for bone regenerative therapies.

DOI： 10.1155/2016/6240794

Scopus

レジンセメントのCAD/CAM用レジンブロックに対する接着強さに及ぼすサンドブラスト処理の影響

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 皆木省吾, 吉原久美子

接着歯学 33 ( 4 ) 181 - 186 2015年12月

記述言語：日本語出版者・発行元：(一社)日本接着歯学会

接着性レジンセメントシステムRelyX Ultimate/Scotchbond Universal Adhesive(3M)の、5種CAD/CAM冠用レジンブロック(Lava Ultimate:3M、CeraSmart:ジーシー、松風HC Block:松風、KZR-CAD HR:山本貴金属地金、KATANA Avencia:クラレノリタケデンタル)に対する接着強さに及ぼすサンドブラストおよびコジェット・サンドブラスト処理の影響を検討した。その結果、サンドブラストあるいはコジェット・サンドブラスト処理することにより、無処理と比較して全てのブロックにおいて有意に接着強さは向上した。ただし、サンドブラスト処理が有効か、コジェット・サンドブラスト処理が有効かは、それぞれのCAD/CAM冠用レジンブロックによって異なることが示された。(著者抄録)

咀嚼による食材の物性変化と嚥下との関係

細坪充裕, 江草正彦, 宮脇卓也, 松本卓也

日本摂食・嚥下リハビリテーション学会雑誌 19 ( 3 ) S287 - S287 2015年12月

Synthesis and modification of apatite nanoparticles for use in dental and medical applications 査読

Masahiro Okada, Takuya Matsumoto

Japanese Dental Science Review 51 ( 4 ) 85 - 95 2015年11月

記述言語：英語出版者・発行元：Elsevier Ltd

Synthesised hydroxyapatite (HAp) exhibits excellent biocompatibility, making it an ideal candidate for use as a hard tissue substitute material. Nanoscale-size effects and surface phenomena impart HAp nanoparticles with unique properties compared to the conventional-sized HAp ceramics. Modification of HAp is also important for regulating its physiochemical properties. In this review, methods of HAp synthesis and modification, and various applications of HAp nanoparticles for dental and medical treatment are discussed.

DOI： 10.1016/j.jdsr.2015.03.004

Scopus

充填用グラスアイオノマーセメント I級窩洞適合性と歯質接着強さ

新規動揺歯固定および矯正用ブラケット接着材のエナメル質に対する接着強さ

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 皆木省吾, 吉原久美子, 長岡紀幸

接着歯学 33 ( 3 ) 143 - 143 2015年11月

最近のレジンセメントの歯質接着性と曲げ特性

丸尾幸憲, 入江正郎, 西川悟郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 33 ( 3 ) 142 - 142 2015年11月

Fluocinolone acetonideとTGF-β3を用いた強力な軟骨再生(Potent cartilage regeneration with fluocinolone acetonide and TGF-β3)

Hara Emilio Satoshi, 大野充昭, Pham Hai Thanh, 久保田聡, 滝川正春, Young Marian F., Olsen Bjorn R., 松本卓也, 窪木拓男

日本バイオマテリアル学会大会予稿集 37回 209 - 209 2015年11月

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 皆木省吾, 長岡紀幸, 吉原久美子

日本歯科理工学会誌 34 ( 5 ) 407 - 407 2015年9月

Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro. 査読国際誌

Hiroaki Taketa, Gulsan Ara Sathi, Mahmoud Farahat, Kazi Anisur Rahman, Takayoshi Sakai, Yoshiaki Hirano, Takuo Kuboki, Yasuhiro Torii, Takuya Matsumoto

Scientific reports 5 11468 - 11468 2015年6月

記述言語：英語掲載種別：研究論文（学術雑誌）

In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication.

DOI： 10.1038/srep11468