研究者詳細 - 西田　圭一郎

論文 - 西田　圭一郎

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Evidence of oncotic cell death and DNA fragmentation in human hypertropic chondrocytes in chondro-osteophyte

Doi, T., Nishida, K., Matsuo, M., Yoshida, A., Murakami, T., Inoue, H.

Osteoarthritis and Cartilage 10 ( 4 ) 2002年

Expression of caspase-3 and-9 relevant to cartilage destruction and chondrocyte apoptosis in human osteoarthritic cartilage

M Matsuo, K Nishida, A Yoshida, T Murakami, H Inoue

ACTA MEDICA OKAYAMA 55 ( 6 ) 333 - 340 2001年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OKAYAMA UNIV MED SCHOOL

To clarify the involvement of the caspase family in the pathway of NO-induced chondrocyte apoptosis, osteoarthritis (OA) cartilage obtained from 8 patients undergoing total hip arthroplasty were used for histopathological study. Cartilage samples taken from non-fibrillated areas of femoral head resected during surgery for femoral neck fracture were used for comparison. DNA fragmentation of chondrocytes was detected by the nick end-labeling (TUNEL) method. Apoptosis was further confirmed by transmission electron microscopy. The distributions of nitrotyrosine (NT), caspase-3, and -9 were examined immunohistochemically. The populations of apoptotic as wen as NT-, caspase-3-, and -9-positive cells were quantified by counting the number of cells in the superficial, middle, and deep layers, respectively. The TUNEL-positive cells were observed primarily in superficial proliferating chondrocytes, clustering chondrocytes, and deep-layer chondrocytes of OA cartilage. Few positive cells were seen in the proliferating chondrocytes in the middle layer. Positive reactions for caspase-3 and -9 were observed in chondrocytes in similar areas. Histological OA grade showed significant correlations with the mean populations of apoptotic chondrocytes (% apoptosis) over the 3 areas. The populations of NT-positive cells (% NT) over the same areas also showed significant correlation with OA grade. Positivity for caspase-3 closely correlated with the OA grade, % apoptosis and %NT. It was concluded that caspase-3 and -9 could play a role in NO-induced chondrocyte apoptosis in OA cartilage.

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Involvement of nitric oxide in chondrocyte cell death in chondro-osteophyte formation

K Nishida, T Doi, M Matsuo, Y Ishiwari, H Tsujigiwa, A Yoshida, M Shibahara, H Inoue

OSTEOARTHRITIS AND CARTILAGE 9 ( 3 ) 232 - 237 2001年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：W B SAUNDERS CO LTD

Objective: To examine the nitric oxide (NO) production relevant to chondrocyte cell death in order to elucidate the mechanism of chondro-osteophyte formation in osteoarthrotic joints.
Design: Human chondra-osteophytes were obtained during total hip arthroplasty. Expression of inducible nitric oxide synthase (iNOS) mRNA was determined by in-situ hybridization. Localization of iNOS and nitrotyrosine at protein level were examined by immunohistochemistry. Cell death of chondrocytes were confirmed by both TUNEL method and transmission electron microscopy.
Results: The various populations of proliferative and hypertrophic chondrocytes expressed iNOS mRNA and iNOS as well as nitrotyrosine protein. Approximately 30% at hypertrophic chondrocytes forming chondro-osteophyte showed positive reaction to TUNEL staining. Electron microscopy confirmed both disintegrated and apoptotic chondrocytes in these zones. In the deep hypertrophic zone calcification was seen around each of the matrix vesicles and some masses of cell debris.
Conclusion: Chondro-osteophyte formation involves NO production by chondrocytes. The expression and localization of iNOS and nitrotyrosine in chondro-osteophytes suggest the significant role of NO in chondrocyte hypertrophy and apoptosis. (C) 2001 OsteoArthritis Research Society international.

DOI： 10.1053/joca.2000.0380

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Reconstruction using clavicle afre resection of arcoma of the proximal humerus.

Ozaki T, Hashizume H, Nishida K, Kawai A, Inoue H

Clin Orthop 2001年

Reconstruction of the proximal humerus with the clavicle after tumor resection: A case report

Ozaki, T., Hashizume, H., Kunisada, T., Kawai, A., Nishida, K., Sugihara, S., Inoue, H.

Clinical Orthopaedics and Related Research ( 385 ) 2001年

Expression of connective tissue growth factor in cartilaginous tumors

89 ( 7 ) 1466 - 1473 2000年10月

CD44 expression in soft tissue sarcomas

Kahara N, Ozaki T, Doi T, Nishida K, Kawai A, Shibahara M, Inoue H

Virchows Arch 436 ( 6 ) 574 - 578 2000年6月

Increased osteocyte apoptosis during the development of femoral head osteonecrosis in spontaneously hypertensive rat

Acta Medica Okayama 54 ( 2 ) 67 - 74 2000年4月

The role of nitric oxide in arthritic joint-a therapeutic target?

Nishida K, Doi T, Inoue H

Modern Rheumatology 10 ( 2 ) 63 - 67 2000年1月

Simultaneous fracture-dislocations of the distal and proximal interphalangeal joints

K Nakago, H Hashizume, M Senda, K Nishida, S Masaoka, H Inoue

JOURNAL OF HAND SURGERY-BRITISH AND EUROPEAN VOLUME 24B ( 6 ) 699 - 702 1999年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHURCHILL LIVINGSTONE

Sixteen cases of simultaneous fracture-dislocations of both the distal interphalangeal (DIP) and proximal interphalangeal (PIP) joints in the same finger that were treated during the past 10 years were classified into three types: the swan-neck injury (dorsal fragment of the base of the distal phalanx at the DIP joint and palmar fragment of the base of the middle phalanx at the PIP joint); the double-hyperextension injury (palmar fragments at the DIP and PIP joints); and the straight-finger injury (with dorsal and palmar bone fragments at the DIP joint). The results of treatment were more satisfactory in PIP joints than in DIP joints.

DOI： 10.1054/jhsb.1999.0228

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Ultrasonographic analysis of shoulder rotator cuff tears

Acta Medic Okayama 53 ( 2 ) 81 - 89 1999年4月

Expression of CD44 variants in osteosarcoma

M. Kuryu, T. Ozaki, K. Nishida, M. Shibahara, A. Kawai, H. Inoue

Journal of Cancer Research and Clinical Oncology 125 ( 11 ) 646 - 652 1999年

記述言語：英語掲載種別：研究論文（学術雑誌）

The standard form of CD44 (CD44H) is a transmembranous glycoprotein, widely distributed on a variety of human lymphoid cells, epithelial cells and tumours. CD44 has many variant forms, which are generated by alternative splicing. In recent years, CD44 has been reported to be related to the degree of tumour differentiation, tumour cell invasion, and metastasis. We investigated 44 tumour specimens in 39 patients with osteosarcoma immunochemically to analyze the expression of CD44 standard (CD44H) and variant exon-encoded gene products (CD44v3, v4, v5, v6, v7, v9, and v10). Furthermore, the relationship between CD44 expression and the clinical outcome of patients with osteosarcoma was analyzed. Membrane accentuation and exclusive cytoplasmic reactivity were analyzed as separate staining patterns. Tumour cells and some multinucleated giant cells were markedly stained. CD44H, v3, v4, v5, v6, v7, v9, and v10 were expressed in 85%, 49%, 54%, 59%, 46%, 5%, 28%, and 10% of the specimens respectively. The cumulative 5-year metastasis-free survival was 58% in CD44v6-negative cases and 24% in CD44v6-positive cases (P = 0.046). However, the cumulative 5-year metastasis-free survival was not significantly different between cases positive and negative for other variants of CD44. Multivariate analysis (Cox proportional-hazard model) with CD44v6 expression (positive or negative), chemotherapy (intensive or non-intensive), tumour site (proximal or distal), and age (at least 30 years or less than 30 years) showed that expression of CD44v6 and chemotherapy were important prognostic factors in patients with osteosarcoma. Overexpression of CD44 isoforms containing variant v6 is correlated with poor prognosis in patients with osteosarcoma.

DOI： 10.1007/s004320050329

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PubMed

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Ultrasonographic analysis of shoulder cuff tearsUltrasonographic analysis of shoulder cuff tears

Masaoka S, Hashizume H, Senda M, Nishida K

Acta MEd Okayama 1999年

Integrin-regulated sevreton of interleukin 4: A novel pathway of mechanotransduction in human articular chondrocytes

Millward-Sadler SJ, Wright MO, Lee H, Nishida K, Caldwell H, Nuki J, Salter DM

J Cell Biol 145 183 - 189 1999年

Evaluation of Rheumatoid Arthritis Using a Scoring System Devised from Magnetic Resonance Imaging of Rheumatoid Knees

Takeuchi Kazuhiro, Inoue Hajime, Yokoyama Yoshiki, Senda Masuo, Ota Yusuke, Abe Nobuhiro, Nishida Keiichiro

Acta Medica Okayama 52 ( 4 ) 211 - 224 1998年8月

記述言語：英語出版者・発行元：Okayama University Medical School

<p>We studied the magnetic resonance imaging (MRI) of 120 knees in 86 rheumatoid arthritis (RA) patients and of 14 unaffected knees in 12 control cases. We also developed a scoring system as a quantitative analysis method. We divided the MRI into 10 items, and classified the severity of the symptoms into 4 grades (score 0 to 3). The average total score increased according to the radiographic grade. Soft tissue lesions were clearly detected, even in the early stages of RA. Items such as synovial proliferation showed a high score even in the early stages, suggesting that it was the initial symptom of RA. The score also showed a correlation with the inflammatory signs. These results suggest that this scoring system is very sensitive and yields a good reflection of RA activity. We demonstrated that this system is simple and convenient for routine diagnostic use. We further demonstrated that it is useful for following the advancement of RA and for evaluating the response to treatment.</p>

DOI： 10.18926/AMO/31296

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Fibrous structure and connection surrounding the metacarpophalangeal joint.

Takagoshi H, Hashizume H, Nishida K, Masaoka S, Asahara H, Inoue H

Acta Medica Okayama 52 ( 1 ) 19 - 26 1998年2月

Mast cells in osteoarthritic and rheumatoid arthritic synovial tissues of the knee.

Acta Medica Okayama 52 ( 1 ) 35 - 39 1998年2月

A Pathogenic role for IL-12 in maintaining Th1 immuneresponses in rheumatoid arthritis.

Morita Y, Yamamura M, Nishida K, Harada S, Okamoto H, Inoue H, Ohmoto Y, Modlin RL, Makino H

Arthritis Rheum 41 306 - 314 1998年

慢性関節リウマチの手指屈筋腱腱鞘炎

太田裕介, 井上一, 橋詰博行, 西田圭一郎, 浅原弘嗣, 臼井正明, 阿部信寛

岡山県立大学短期大学部研究紀要 ( 5 ) 37 - 44 1998年

記述言語：日本語出版者・発行元：岡山県立大学短期大学部

［概要］慢性関節リウマチ（RA）において手指屈筋腱腱鞘炎（腱鞘炎）を起こす例をしばしば認めるため，横断調査を行った。［対象，方法］岡山大学整形外科リウマチ外来に通院している52例（男６例，女46例）で平均年齢59.5歳（24～85歳），平均罹病期間9.2年（2～30年）を対象とし，視診および触診にて腱鞘炎，弾発現象，ロッキングの有無，および弾発現象の既往につき調査した。調査期間は平成９年４月～５月の２ヵ月間であった。［結果］腱鞘炎を25例（48％）63指に認めた。全63指中，母指～小指が各々4，12，14，19，14指と環指に多かった。１指発生が10例と最も多かったが８指発生も２例あり，うち１例は両手指廃用であった。弾発現象を９例（17％）11指に認め，中指２指，環指５指，小指４指と環指に多い傾向を認めた。ロッキングは４例（８％）５指に認め，手指基節掌側に３例，母指基節掌側に１例認めたが，痛みはなく，日常生活動作の障害はわずかであった。弾発現象の既往のある25例中２例が手術を受けていたが９例（17％）が放置にて治癒していた。また４例はRAの初発症状として弾発現象を認めた。［考察］弾発現象は放置しても自然に消失することが多いので，短期的には経過観察でもよいが，腱断裂を起こす例もあるので，注意が必要と思われた。［まとめ］日常診療において手指屈筋腱腱鞘炎の存在に留意し，腱鞘内注射が有効でない場合は手術的治療も考慮すべきと思われた。

DOI： 10.15009/00000959

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その他リンク： http://id.nii.ac.jp/1212/00000959/

慢性関節リウマチにおける手関節のＭＲＩ

正岡俊二, 橋詰博行, 西田圭一郎, 浅原弘嗣, 井上一

中国・四国整形外科学会雑誌 10 ( 2 ) 325 - 329 1998年

記述言語：日本語出版者・発行元：The Chugoku-Shikoku Orthopaedic Association

Magnetic resonance imaging (MRI) was performed in 71 wrists of 39 rheumatoid arthritis patients. There were 2 males (3 wrists) and 37 females (68 wrists) with an average age of 55.4 years. Synovial proliferation and bone erosion appeared as low intensity areas on T1-weighted MRIs and as high intensity areas on T2-weighted images. Discrimination of synovial proliferation was improved by Gd-DTPA enhancement. These lesions could be detected in early rheumatoid arthritis patients whose plain X-ray images lacked abnormal findings. We found MRI to be a useful method for detecting bone and soft tissue lesions of RA, especially of early RA patients. MRI is also useful in evaluation of drug therapy and pre-operative planning.

DOI： 10.11360/jcsoa1989.10.325

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