研究者詳細 - 西田　圭一郎

論文 - 西田　圭一郎

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Computer Navigation-Assisted Spinal Fusion with Segmental Pedicle Screw Instrumentation for Scoliosis with Rett Syndrome: A Case Report

Masato Tanaka, Kazuo Nakanishi, Yoshihisa Sugimoto, Haruo Misawa, Tomoyuki Takigawa, Keiichiro Nishida, Toshifumi Ozaki

ACTA MEDICA OKAYAMA 63 ( 6 ) 373 - 377 2009年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OKAYAMA UNIV MED SCHOOL

Scoliosis is a common clinical manifestation of Rett syndrome, a neurodevelopmental disorder that almost exclusively affects females. The spinal curve in patients with Rett syndrome is typically a long C curve of a neuromuscular type. As the onset of the scoliosis is very early and shows rapid progression, early surgical intervention has been recommended to prevent a life-threatening collapsing spine syndrome. However, there are high perioperative risks in Rett syndrome patients who undergo spinal surgery, such as neurological compromise and respiratory dysfunction due to rigrid spinal curve. We herein report the surgical result of treating severe rapid progressive thoracic scoliosis in a 16-year-old girl with Rett syndrome. Posterior segmental pedicle screw fixation was performed from T1 to L3 using a computer-assisted technique. Post-operative radiography demonstrated a good correction of the curve in both the sagittal and coronal alignment. There were no postoperative complications such as neurological compromise. The patient had maintained satisfactory spinal balance as of the 3-year follow-up examination.

DOI： 10.18926/AMO/31823

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A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) expression by chondrocytes during endochondral ossification

Kanae Kumagishi, Keiichiro Nishida, Tomoichiro Yamaai, Ryusuke Momota, Shigeru Miyaki, Satoshi Hirohata, Ichiro Naito, Hiroshi Asahara, Yoshifumi Ninomiya, Aiji Ohtsuka

ARCHIVES OF HISTOLOGY AND CYTOLOGY 72 ( 3 ) 175 - 185 2009年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：INT SOC HISTOLOGY & CYTOLOGY

A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) is known to influence aggrecan degradation in endochondral ossification, but its role has not been well understood. In the present study, in vitro gene expression of ADAMTS9 was investigated by RT-PCR in ATDC5 cells in which experimentally chondrogenic differentiation had been induced. We also investigated the protein localization and gene expression pattern of ADAMTS9 in the tibia growth plate cartilage of male mice in a day 1 neonate, 7-week-old young adult, and a 12-week-old adult by immunohistochemistry and in situ hybridization and compared the results with the expression of proliferating cell nuclear antigen (PCNA) and type X collagen for the identification of proliferative and hypertrophic chondrocyte phenotypes, respectively. We found the gene expression of ADAMTS9 by ATDC5 cells as a dual mode, both before the expression of type X collagen and after hypertrophic differentiation. The immunoreactivity of ADAMTS9 was observed in chondrocytes of proliferative and hypertrophic zones in the growth plate. The population of ADAMTS9 positive cells decreased with age. The results of the present study suggest that ADAMTS9 might have a role in aggrecan cleavage around the chondrocytes to allow chondrocyte proliferation and hypertrophy.

DOI： 10.1679/aohc.72.175

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Mechanical stretch stimulates integrin αVβ3-mediated collagen expression in human anterior cruciate ligament cells

Tetsunaga T, Furumatsu T, Abe N, Nishida K, Naruse K, Ozaki T

Journal of Biomechanics 42 ( 13 ) 2097 - 2103 2009年9月

Optical imaging of mouse articular cartilage using the glycosaminoglycans binding property of fluorescent-labeled octaarginine

K. Inagawa, T. Oohashi, K. Nishida, J. Minaguchi, T. Tsubakishita, K. O. Yaykasli, A. Ohtsuka, T. Ozaki, T. Moriguchi, Y. Ninomiya

OSTEOARTHRITIS AND CARTILAGE 17 ( 9 ) 1209 - 1218 2009年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：W B SAUNDERS CO LTD

Objective: The aim of the current study was to examine the cartilage-specific binding property of polyarginine peptides (R4, 8, 12, and 16) and specifically to test octaarginine peptides for the optical imaging of articular cartilage in experimentally induced arthritis in mice.
Methods: Four rhodamine-labeled polyarginine peptides each with a different-length arginine chain (R4, 8, 12, or 16) were injected into the knee joints of C57BL/6J mice (n = 20). The joints were excised 1 h later and the fluorescent signal intensity in cartilage cryosections was compared for the four peptides. To examine the substrate of R8 in cartilage, femoral condyles obtained from another set of mice were treated with chondroitinase ABC (Ch'ase ABC), keratanase or heparitinase then immersed in R8-rhodamine. Fluorescent signals were examined by fluorescent microscopy. Next, R8-rhodamine was injected into the right knee joints of three control and three collagen antibody-induced arthritis (CAIA) mice, and fluorescent intensity in normal and degenerative cartilage was semi-quantitatively analysed on the histological sections using image software. Finally, femoral condyles from normal mice (n = 2) and CAIA mice (n = 2) were immersed in R8-rhodamine and calcein, then imaged using optical projection tomography (OPT).
Results: Fluorescent signals were specifically detected in the cartilage pericellular matrix from the surface to the tide mark but were completely absent in the calcified layer or bone marrow. The number of arginine residues significantly influenced peptide accumulation in articular cartilage, with R8 accumulating the most. The fluorescent signal in the femoral condylar cartilage diminished when it was treated with Ch'ase ABC. R8 accumulation was significantly decreased in the degenerative cartilage of CAIA mice, and this was demonstrated both histologically and in three-dimensional (3D)-reconstruction image by OPT.
Conclusion: R8 may be a useful new experimental probe for optical imaging of normal and arthritic articular cartilage. (C) 2009 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.joca.2009.03.010

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【整形外科関節リウマチ保存治療の実際】RA診断・治療におけるMRIの有用性

中原龍一, 西田圭一郎

Orthopaedics 22 ( 9 ) 31 - 38 2009年8月

記述言語：日本語出版者・発行元：(株)全日本病院出版会

関節リウマチ(RA)に対する治療ガイドラインが策定され、生物学的製剤の登場やメトトレキサートの積極的使用により、早期診断、薬効評価における画像診断の重要性が大きくなりつつある。MRI画像によって得られるRAに特徴的な所見としては、滑膜炎、骨びらん、骨髄浮腫、腱鞘滑膜炎などがあり、補助診断としてRAを早期に診断し、予後不良因子などをもとに治療法を決定し、寛解・薬剤中止を目指すためのツールとしてのエビデンスが蓄積されつつある。早期診断においてはfalse positiveの除外を含めた正しい評価方法を知ることが重要であり、薬効判定においては滑膜体積や最大造影速度などを用いた定量的判定が求められている。(著者抄録)

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マウス成長軟骨におけるADAMTS-9発現とその役割について

熊岸加苗, 西田圭一郎, 百田龍輔, 山合友一朗, 廣畑聡, Kadir Demircan, 内藤一郎, 二宮善文, 大塚愛二

解剖学雑誌 84 ( Suppl. ) 139 - 139 2009年3月

Target-tropic accumulation of SLX-liposome in arthritis mouse 査読

Minaguchi JA, Minematsu H, Oohashi T, Otani T, Inagawa K, Oie K, Nishida K, Ohtsuka A, Ninomiya Y, Igarashi K

Matrix Biology 27 43 - 43 2008年12月

The expression of microRNA424 in rheumatoid arthritis

Yoshitaka, Teruhito, Nakasa, Tomoyuki, Inoue, Atsushi, Okubo, Atsuko, Takemoto, Fuko, Miyaki, Shigeru, Nishida, Keiichiro, Ochi, MitStio, Ozaki, Toshifumi, Sumida, Takayuki, Asahara, Hiroshi

ARTHRITIS AND RHEUMATISM 58 ( 9::Suppl. S ) 0 - 0 2008年9月

Disease-specific screening for deep venous thrombosis and pulmonary thromboembolism using plasma D-dimer values after total knee arthroplasty

Teruhito Yoshitaka, Nobuhiro Abe, Hiroshi Minagawa, Hirokazu Date, Yoshimasa Sakoma, Keiichiro Nishida, Toshifumi Ozaki

MODERN RHEUMATOLOGY 18 ( 4 ) 359 - 365 2008年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER

We prospectively evaluated the disease-specific features of the early postoperative plasma D-dimer value and the relationship with deep venous thrombosis and/or pulmonary thromboembolism (DVT/PE) in 95 patients following total knee arthroplasty. Patients in whom DVT/PE was highly suspected were diagnosed by high-resolution multi-detector row computed tomography scanning (MDCT). Forty-nine knees in 46 patients with rheumatoid arthritis (RA, 24 knees) or osteoarthritis (OA, 25 knees) were finally recruited. DVT/PE was detected in 28 (57.1%) of the 49 cases examined by diagnostic MDCT: 12 (50.0%) of the 24 cases of RA, and 16 (64.0%) of the 25 cases of OA. Of these, PE was found in 11 cases (39.2%), but none of them showed clinical symptomatic signs of dyspnea or chest pain. In both RA and OA cases, there were statistically significant differences in the D-dimer value on postoperative day 3 (P = 0.027) and after day 28 (P = 0.037) between the groups with and without DVT/PE. In OA cases, there were significant differences between the two groups on postoperative days 1 (P = 0.034), 3 (P = 0.020), 5 (P = 0.005), and 7 (P = 0.045), respectively. At the baseline, perioperative D-dimer levels in the RA group without DVT/PE were higher than in the OA group. However, multivariate logistic regression analysis showed that RA was not a significant risk factor of DVT/PE in comparison with OA. In conclusion, individual evaluation of the D-dimer level between RA and OA should provide a more precise predictive indicator of early postoperative DVT/PE.

DOI： 10.1007/s10165-008-0068-6

Intra-articular injection of interleukin-4 decreases nitric oxide production by chondrocytes and ameliorates subsequent destruction of cartilage in instability-induced osteoarthritis in rat knee joints

M. Yorimitsu, K. Nishida, A. Shimizu, H. Doi, S. Miyazawa, T. Komiyama, Y. Nasu, A. Yoshida, S. Watanabe, T. Ozaki

OSTEOARTHRITIS AND CARTILAGE 16 ( 7 ) 764 - 771 2008年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

Objective: To investigate the in vitro and in vivo effects of interleukin (IL)-4 on mechanical stress-induced nitric oxide (NO) expression by chondrocytes, and destruction of cartilage and NO production in an instability-induced osteoarthritis (OA) model in rat knee joints, respectively.
Materials and methods: Cyclic tensile stress (CTS; 0.5 Hz and 7% elongation) was applied to cultured normal rat chondrocytes with or without pre-incubation with recombinant rat IL-4 (rrIL-4). Inducible NO synthase (iNOS) mRNA expression and NO production were examined with real-time polymerase chain reaction and the Griess reaction, respectively. OA was induced in rat knee joints by transection of the anterior cruciate and medial collateral ligaments and resection of the medial meniscus. rrIL-4 (10, 50, and 100 ng/joint/day) was injected intra-articularly, and knee joint samples were collected 2, 4, and 6 weeks after surgery. Cartilage destruction was evaluated by the modified Mankin score and Osteoarthritis Research Society International scoring system on paraffin-embedded sections stained with safranin O. Cleavage of aggrecan and NO production were examined by immunohistochemistry for aggrecan neoepitope (NITEGE) and of nitrotyrosine (NT), respectively.
Results: rrIL-4 down-regulated CTS-induced iNOS mRNA expression and NO production by chondrocytes. The intra-articular injection of rrIL-4 gave rise to a limited, but significant amelioration of cartilage destruction, prevention of loss of aggrecan, and decrease in the number of NT-positive chondrocytes, an effect that was not dose-dependent.
Conclusion: The present study suggests that IL-4 may exert chondroprotective properties against mechanical stress-induced cartilage destruction, at least in part, by inhibiting NO production by chondrocytes. (C) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.joca.2007.11.006

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Novel magnetic resonance imaging evaluation for valgus instability of the knee caused by medial collateral ligament injury

Hisanori Ikuma, Nobuhiro Abe, Youichiro Uchida, Takayuki Furumatsu, Kazuo Fujiwara, Keiichiro Nishida, Toshifumi Ozaki

ACTA MEDICA OKAYAMA 62 ( 3 ) 185 - 191 2008年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OKAYAMA UNIV MED SCHOOL

Instability of the knee after the medial collateral ligament (MCL) injury is usually assessed with the manual valgus stress test, even though, in recent years, it has become possible to apply magnetic resonance imaging (MRI) to the assessment of the damage of the ligament. The valgus instability of 24 patients (12 isolated injuries and 12 multiple ligament injuries) who suffered MCL injury between 1993 and 1998 was evaluated with the Hughston and Eilers classification, which involves radiographic assessment under manual valgus stress to the injured knees. We developed a novel system for classifying the degree of injury to the MCL by calculating the percentage of injured area based on MRI and investigated the relationship between this novel MRI classification and the magnitude of valgus instability by the Hughston and Eilers classification. There was a significant correlation between the 2 classifications (p = 0.0006). On the other hand, the results using other MRI based classification systems, such as the Mink and Deutsch classificaiton and the Petermann classification, were not correlated with the findings by the Hughston and Eilers classification in these cases (p > 0.05). Since MRI is capable of assessing the injured ligament in clinical practice, this novel classification system would be useful for evaluating the stability of the knee and choosing an appropriate treatment following MCL injury.

DOI： 10.18926/AMO/30974

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Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue

Tomoyuki Nakasa, Shigeru Miyaki, Atsuko Okubo, Megumi Hashimoto, Keiichiro Nishida, Mitsuo Ochi, Hiroshi Asahara

ARTHRITIS AND RHEUMATISM 58 ( 5 ) 1284 - 1292 2008年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY-LISS

Objective. Several microRNA, which are similar to 22-nucleotide noncoding RNAs, exhibit tissue-specific or developmental stage-specific expression patterns and are associated with human diseases. The objective of this study was to identify the expression pattern of microRNA-146 (miR-146) in synovial tissue from patients with rheumatoid arthritis (RA).
Methods. The expression of miR-146 in synovial tissue from 5 patients with RA, 5 patients with osteoarthritis (OA), and 1 normal subject was analyzed by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and by in situ hybridization and immunohistochemistry of tissue sections. Induction of miR-146 following stimulation with tumor necrosis factor alpha (TNF alpha) and interleukin-1 beta (IL-1 beta) of cultures of human rheumatoid arthritis synovial fibroblasts (RASFs) was examined by quantitative PCR and RT-PCR.
Results. Mature miR-146a and primary miR146a/b were highly expressed in RA synovial tissue, which also expressed TNF alpha, but the 2 microRNA were less highly expressed in OA and normal synovial tissue. In situ hybridization showed primary miR-146a expression in cells of the superficial and sublining layers in synovial tissue from RA patients. Cells positive for miR-146a were primarily CD68+ macrophages, but included several CD3+ T cell subsets and CD79a+ B cells. Expression of miR-146a/b was markedly upregulated in RASFs after stimulation with TNF alpha and IL-1 beta.
Conclusion. This study shows that miR-146 is expressed in RA synovial tissue and that its expression is induced by stimulation with TNF alpha and IL-1 beta. Further studies are required to elucidate the function of miR-146 in these tissues.

DOI： 10.1002/art.23429

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Interleukin-4 downregulates the cyclic tensile stress-induced matrix metalloproteinases-13 and cathepsin B expression by rat normal chondrocytes

Hideyuki Doi, Keiichiro Nishida, Masanori Yorimitsu, Takamitsu Komiyama, Yasutaka Kadota, Tomonori Tetsunaga, Aki Yoshida, Satoshi Kubota, Masaharu Takigawa, Toshifumi Ozaki

ACTA MEDICA OKAYAMA 62 ( 2 ) 119 - 126 2008年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OKAYAMA UNIV MED SCHOOL

Mechanical stress plays a key role in the pathogenesis of cartilage destruction seen in osteoarthritis (OA). We investigated the effect of cyclic tensile stress (CTS) on the anabolic and catabolic gene expression of rat cultured normal chondrocytes using the Flexercell strain unit. The effects of interleukin (IL)-4, a chondroprotective cytokine, on the changes in gene expression induced by CTS were also investigated. CTS (7% elongation at 0.5 Hz) for 24 h did not affect the expression of aggrecan and type 11 collagen, whereas CTS significantly upregulated matrix metalloproteinase (MMP)-13 and cathepsin B mRNA expression by chondrocytes. IL-1 beta expression was also significantly upregulated by CTS up to 12 h. The upregulation of MMP-13 was observed at 3 h, which was earlier than that of IL-1 beta. Furthermore, pre-treatment with IL-4 (10 ng/ml) suppressed both MMP-13 and cathepsin B induction by mechanical stress, as well as CTS-induced IL-1 beta expression. Our results suggest that IL-4 might have a therapeutic value in the treatment of OA by downregulation of mechanical stress-induced MMP-13 and cathepsin B expression by chondrocytes.

DOI： 10.18926/AMO/30956

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Application of laminar screws to posterior fusion of cervical spine

Kazuo Nakanishi, Masato Tanaka, Yoshihisa Sugimoto, Haruo Misawa, Tomoyuki Takigawa, Kazuo Fujiwara, Keiichiro Nishida, Toshifumi Ozaki

SPINE 33 ( 6 ) 620 - 623 2008年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：LIPPINCOTT WILLIAMS & WILKINS

Study Design. Morphometric analysis.
Objective. For safe and solid fixation, it is necessary to measure the diameter of the vertebral arch to ascertain whether or not screws can be used and if so, the appropriate size of screws to be used.
Summary of Background Data. Cervical pedicle screws are the most biomechanically stable screws. However, their use carries a high risk of neurovascular complications during screw insertion. In 2004, a new method to avoid such vertebral artery injuries was reported by insertion of screws with crosswise to the lamina of C2. For safe and solid fixation, it is necessary to measure the diameter of the vertebral arch to ascertain whether or not screws can be used and if so, the appropriate size of screws to be used. There is no report of the diameter of the vertebral arch by a navigation system.
Methods. Morphometric analysis was performed on 42 patients who had undergone a CT scan of the cervical spine for either surgery or diagnostic purposes. To examine the possibilities to insert laminar screws, the diameter of the vertebral arch was measured using a navigation system.
Results. The diameter of the vertebral arch in C2 was the largest in the cervical spine, individual differences ranging between 0.8 and 8.4 mm. In C2, insertion of screws with a diameter of 3 mm was possible in 80% of males and 63% of females. As for screws with a diameter of 4 mm, insertion was possible in 50% of the males and 24% of the females in C2. In C2, gender had a significant effect, but left-right differences and height did not.
Conclusion. Laminar screws are useful as they can prevent vascular injuries, but a preoperative evaluation is necessary.

DOI： 10.1097/BRS.0b013e318166aa76

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Inhibition of histone deacetylase down-regulates the expression of hypoxia-induced vascular endothelial growth factor by rheumatoid synovial fibroblasts

H. Manabe, Y. Nasu, T. Komiyama, T. Furumatsu, A. Kitamura, S. Miyazawa, Y. Ninomiya, T. Ozaki, H. Asahara, K. Nishida

INFLAMMATION RESEARCH 57 ( 1 ) 4 - 10 2008年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：BIRKHAUSER VERLAG AG

Objective: To investigate the effect of FK228 on the in vitro expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) by rheumatoid arthritis synovial fibroblasts (RASFs), and on the in vivo expression of VEGF and angiogenesis in the synovial tissue of mice with collagen-antibody-induced arthritis (CAIA).
Methods: RASFs were stimulated with IL-1 beta and TNF alpha and then incubated under hypoxia (1 % O-2) with various concentrations of FK228. The effects of FK228 on the expression of HIF-1 alpha and VEGF mRNA were examined by quantitative real-time PCR. Changes in HIF-1 alpha protein expression and the secretion of VEGF protein into the culture medium were examined by Western blot analysis and ELISA, respectively. Immunohistochemical analysis was carried out to investigate the expression and distribution of VEGF in synovial tissues of CAIA mice.
Results: The cytokine-stimulated expression of HIF-1 alpha and VEGF mRNA was inhibited by FK228 in a dose-dependent manner. FK228 also reduced the expression of HIF-1 alpha and VEGF protein. Intravenous administration of FK228 (2.5 mg/kg) suppressed VEGF expression, and also blocked angiogenesis in the synovial tissue of CAIA.
Conclusion: FK228 may exhibit a therapeutic effect on RA by inhibition of angiogenesis through down-regulation of angiogenesis related factors, HIF-1 alpha and VEGF.

DOI： 10.1007/s00011-007-7036-z

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Isolation of a mRNA preferentially expressed in synoviocytes from rheumatoid arthritis that is identical with lumican, which encodes a collagen binding Extracellular Matrix Protein 査読

Hiroki Mori, Keiichiro Nishida, Toshifumi Ozaki, Hajime Inoue, Tohru Nakanishi

Journal of Hard Tissue Biology 17 ( 3 ) 125 - 130 2008年

記述言語：英語掲載種別：研究論文（学術雑誌）

Rheumatoid arthritis (RA) is a complex disease including autoimmune disorder and resulting in inflammation. It also shows progressive proliferation of synoviocytes, and these synoviocytes destroys articular structure. In order to understand the mechanism of this abnormal proliferation of RA-originated synoviocytes in molecular level, we analyzed the gene expression profiles by using DNA chips that contain more than 10,000 genes. Comparing the expression profiles of normal and RA-originated synoviocytes, we found several genes that are highly expressed in RA-originated synoviocytes than normal synoviocytes. Among these genes, we focused on one hypothetical protein, cDNA of which contains one reading frame in its DNA fragment, indicating that this fragment is a part of large mRNA structure. The expression of this gene in RA-originated synoviocytes is about three times higher than that in normal synoviocytes by DNA chip analysis. After cDNA cloning of this mRNA, we found that the gene is identical with lumican, which encodes a collagen binding, extracellular matrix protein. This mRNA was widely distributed in many tissues but its alternatively spliced forms are differently expressed in various tissues. ©2008 The Hard Tissue Biology Network Association Printed in Japan, All rights reserved.

DOI： 10.2485/jhtb.17.125

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Chondrosarcoma and Peroxisome Proliferator-Activated Receptor

K. Nishida, T. Kunisada, Z. N. Shen, Y. Kadota, K. Hashizume, T. Ozaki

PPAR RESEARCH 2008 1 - 7 2008年

記述言語：英語出版者・発行元：HINDAWI PUBLISHING CORPORATION

Induction of differentiation and apoptosis in cancer cells by ligands of PPAR gamma is a novel therapeutic approach to malignant tumors. Chondrosarcoma (malignant cartilage tumor) and OUMS-27 cells (cell line established from grade III human chondrosarcoma) express PPAR gamma. PPAR gamma ligands inhibited cell proliferation in a dose-dependent manner, and induced apoptosis of OUMS-27. The higher-grade chondrosarcoma expressed a higher amount of antiapoptotic Bcl-xL in vivo. The treatment of OUMS-27 by 15d-PGJ(2), the most potent endogenous ligand for PPAR gamma, downregulated expression of Bcl-xL and induced transient upregulation of proapoptotic Bax, which could accelerate cytochrome c release from mitochondria to the cytosol, followed by induction of caspase-dependent apoptosis. 15d-PGJ(2) induced the expression of CDK inhibitor p21 protein in human chondrosarcoma cells, which appears to be involved in the mechanism of inhibition of cell proliferation. These findings suggest that targeted therapy with PPAR gamma ligands could be a novel strategy against chondrosarcoma. Copyright (C) 2008 K. Nishida et al.

DOI： 10.1155/2008/250568

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Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced arthritis mouse model

Nasu Y, Nishida K, Miyazawa S, Komiyama T, Kadota Y, Abe N, Yoshida A, Hirohata S, Ohtsuka A, Ozaki T

Osteoarthritis and Cartilage 16 ( 6 ) 723 - 732 2008年

The distribution of type IV collagen α chains in basement membranes of human epidermis and skin appendages

Hasegawa H, Naito I, Nakano K, Momota R, Nishida K, Taguchi T, Sado Y, Ninomiya Y, Ohtsuka A

Arch Histol Cytol 70 ( 4 ) 255 - 265 2007年11月

New approach for assessing vascular distribution within bone tumors using dynamic contrast-enhanced MRI

Yukio Kawakami, Toshiyuki Kunisada, Shinsuke Sugihara, Atsushi Ono, Keiichiro Nishida, Nobuhiro Abe, Akira Kawai, Kazuo Fujiwara, Yuki Morimoto, Toshifumi Ozaki

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 133 ( 10 ) 697 - 703 2007年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER

Purpose To differentiate benign from malignant bone tumors by analyzing the vascular distribution within bone tumors with dynamic contrast-enhanced MRI.
Methods We studied dynamic contrast-enhanced MRI for 49 bone tumors (22 malignant and 27 benign tumors). Seven small regions of interest (ROI) were set inside the largest portion of each tumor. Four ROI were placed evenly on the periphery and three ROI were placed evenly on the line of the longest breadth within the tumor. The slope of the curve (%Slope) was calculated on the time-intensity curves of the whole tumor and of each ROI. The variance values for the %Slope of the ROI were calculated to assess the dispersion of the intensity change at each ROI within the tumor.
Results Mean value of the %Slopes of whole tumor regions for malignant bone tumors (70.4 +/- 60.3%) was significantly higher than that for benign bone tumors (37.6 +/- 52.9%) (P = 0.015), although giant cell tumor (GCT), a locally aggressive tumor, had a relatively higher %Slope. Mean value of the variance of %Slopes for malignant bone tumors (3485.9 +/- 5942.5) was significantly higher than that for all benign tumors (470.4 +/- 583.9) (P = 0.012), indicating that the %Slope values of seven ROI within malignant bone tumors varied more widely compared with the ROI inside benign bone tumors. GCT also demonstrated a lower value.
Conclusion Our method of analyzing the signal intensity change at seven separate regions that evaluates the vascular distribution within a tumor could be a useful tool for differentiating between benign and malignant bone tumors.

DOI： 10.1007/s00432-006-0190-9

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