研究者詳細 - 三村　由香里

MISC - 三村　由香里

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Japanese family with glucocorticoid-remediable aldosteronism diagnosed by long-polymerase chain reaction

K Yokota, T Ogura, M Kishida, J Suzuki, F Otsuka, Y Mimura, T Oishi, M Hirata, K Tobe, H Makino

HYPERTENSION RESEARCH 24 ( 5 ) 589 - 594 2001年9月

記述言語：英語出版者・発行元：JAPANESE SOC HYPERTENSION CENT ACADEMIC SOC, PUBL OFFICE

We report a Japanese family with glucocorticoid-remediable aldosteronism (GRA) in whom gene abnormality was identified by the long-polymerase chain reaction (PCR) method. The proband was a 21-year-old female incidentally found to have high blood pressure (173/107 mmHg). Laboratory tests showed hypokalemia (3.7 mmol/l), and high plasma aldosterone concentration (PAC, 234 pg/ml) with suppressed plasma renin activity (PRA, < 0.1 ng/ml/h). The circadian rhythm pattern and the results of a rapid adrenocorticotrophic hormone (ACTH) test indicated ACTH-dependent changes in PAC. Imaging studies showed no adrenal mass on either side. A dexamethasone (Dexa) suppression test (1.0 mg/day orally for 7 days) showed a marked decrease of PAC 2 days after administration, and this decreased level was maintained throughout Dexa administration. High blood pressure and hypokalemia also improved during Dexa treatment. The proband's younger sister was 19 years old and had hypertension, PAC of 231 pg/ml, and PRA < 0.1 ng/ml/h. The mother was 53 years old and had hypertension, PAC of 98.5 pg/ml, and PRA < 0.1 ng/ml/h. The proband's elder sister was a 22-year-old normotensive with PAC of 110 pg/ml and PRA of 0.1 ng/ml. Long-PCR was performed for detection of the chimeric gene associated with GRA, using DNA samples from all four cases and two normal control subjects. Although the aldosterone synthase gene was expressed among all DNA samples, the chimeric gene was detected only in the proband, her younger sister and her mother. Our clinical data and genetic investigation confirmed the presence of GRA in this Japanese family.

DOI： 10.1291/hypres.24.589

Chronic treatment with amlodipine modulates adrenocortical angiotensin II receptors in spontaneously hypertensive rats

M Kishida, F Otsuka, T Ogura, H Kataoka, Y Nakamura, T Yamauchi, M Takahashi, K Yokota, Y Mimura, H Makino

LIFE SCIENCES 69 ( 1 ) 113 - 122 2001年5月

記述言語：英語出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

We investigated the effects of long-term treatment with calcium-antagonist, amlodipine, on angiotensin II receptors in the adrenal cortex of spontaneously hypertensive rats (SHR). Seven-week-old male SHR were treated with oral amlodipine (10 mg/kg/day) or vehicle (saline) for four weeks. Age-matched normotensive Wistar-Kyoto (WKY) rats were treated with the vehicle similar to control SHR. Systolic blood pressure (SBP) showed time-dependent increase in SHR but not in WKY rats, while amlodipine treatment significantly reduced the high SEP in SHR, Plasma renin activity was serially increased in SHR, which was further enhanced by amlodipine treatment, But the plasma aldosterone level which was increased in SHR was not changed by amlodipine. Competitive reverse transcriptase-polymerase chain reaction showed that the level of adrenocortical angiotensin II type 1 receptor (AT1R) mRNA progressively decreased in vehicle-treated SHR compared to WKY rats and that 4-week course of amlodipine treatment significantly increased AT1R mRNA in SHR to levels comparable to those in WKY rats. Amlodipine treatment reduced the level of adrenocortical angiotensin II type 2 receptor (AT2R) mRNA in SHR from 8 weeks of age. Thus, chronic amlodipine treatment differently modulates both adrenocortical AT1R and AT2R in SHR in a possibly direct manner, (C) 2001 Elsevier Science Inc. All rights reserved.

DOI： 10.1016/S0024-3205(01)01071-2

Coexistence of Graves' disease and struma ovarii: Case report and literature review

Y Mimura, M Kishida, H Masuyama, N Suwaki, J Kodama, F Otsuka, H Kataoka, T Yamauchi, T Ogura, T Kudo, H Makino

ENDOCRINE JOURNAL 48 ( 2 ) 255 - 260 2001年4月

記述言語：英語掲載種別：書評論文，書評，文献紹介等出版者・発行元：JAPAN ENDOCRINE SOCIETY

We report a rare case of Graves' disease associated with struma ovarii. A 26-year-old Japanese woman had preexisting Graves' disease and was positive for TSH receptor antibody. She had been on antithyroid medication at presentation. She noted a mass in the lower left abdomen, which was diagnosed as a left struma ovarii by radiological work-up including computed tomography, magnetic resonance imaging and scintigraphy. The surgically excised teratomatous tumor, containing cystic spaces with thyroid tissue, was histologically proved to be struma ovarii. Since thyroid function tests and TSH receptor antibody did not change after surgery, her hyperthyroidism was considered to be due to Graves' disease. Our case was diagnosed as struma ovarii before surgery using various imaging studies.

DOI： 10.1507/endocrj.48.255

The role of nitric oxide and the renin-angiotensin system in salt-restricted Dahl rats

H Kataoka, F Otsuka, T Ogura, T Yamauchi, M Kishida, M Takahashi, Y Mimura, H Makino

AMERICAN JOURNAL OF HYPERTENSION 14 ( 3 ) 276 - 285 2001年3月

記述言語：英語出版者・発行元：ELSEVIER SCIENCE INC

To elucidate the role of nitric oxide (NO) and renin angiotensin system (RAS) in the development of salt-sensitive hypertension, we investigated the presser responses and renal histologic changes after long-term inhibition of endogenous NO synthesis in Dahl-Iwai salt-sensitive (DS) and salt-resistant (DR) rats under salt-restricted conditions that exaggerate RAS activation. Male DS and DR rats (6 weeks old) were fed with a low-salt (0.3%) diet for 5 weeks, N-G-nitro-L-arginine (L-NA: dissolved in 60 mg/L deionized water), an arginine analogue acting as a NO-inhibitor, was also administered for 5 weeks. L-NA administration induced a gradual increase in systolic blood pressure (SBP) in both strains, and the presser response in DS rats was apparently more enhanced relative to that in DR rats. Urinary nitrate plus nitrite (u-NOx) excretion was decreased by L-NA, with a significant negative correlation between SEP and u-NOx excretion in DS rats but not in DR rats. Plasma renin activity and urinary aldosterone level were significantly increased in L-NA-treated DS rats on week 5. Marked histologic changes with glomerular sclerosis and increased protein-uria and urinary N-acetyl-beta -glucosaminidase excretion were found in L-NA-treated DS rats but not DR rats. Competitive RT-PCR of mRNA extracted from the glomeruli revealed that angiotensin II type 1 receptor (AT(1)R) mRNA level was significantly lower in DS rats than in DR rats at week 2, and that L-NA administration significantly reduced glomerular AT(1)R level of DS rats at week 5, possibly because of downregulation. Our results showed that, even under sodium restriction, the presser response and renal injury induced by chronic NO inhibition were markedly more enhanced in DS rats than in DR rats, which indicates that depletion of NO participates in both the development of hypertension and glomerular injury in DS rats through a potential activation of RAS irrespective of sodium loading. These data suggest that endogenous NO is an essential determinant of salt-sensitive hypertension in DS rats. (C) 2001 American Journal of Hypertension, Ltd.

DOI： 10.1016/S0895-7061(00)01255-3

当大学における10年間の甲状腺疾患ﾑ昨年経験した甲状腺癌３例を中心にー

小倉俊郎, 松浦一陽, 塚本千秋, 絹見佳子, 内藤恵子, 福森明美, 今井あゆみ, 岸田雅之, 戸部和夫, 三村由香里

CAMPUS HEALTH 2001年

Hyperthyroidism in a patient with TSH-producing pituitary adenoma coexisting with thyroid papillary adenocarcinoma

M Kishida, F Otsuka, H Kataoka, K Yokota, T Oishi, T Yamauchi, H Doihara, T Tamiya, Y Mimura, T Ogura, H Makino

ENDOCRINE JOURNAL 47 ( 6 ) 731 - 738 2000年12月

記述言語：英語出版者・発行元：JAPAN ENDOCRINE SOCIETY

A 27-year-old woman who presented with a left thyroid nodule was found to have hyperthyroidism caused by a syndrome of inappropriate secretion of TSH. The levels of free T-3, free T-4 and TSH were 9.50 pg/mL, 4.05 ng/dL and 2.16 muU/mL, respectively. Magnetic resonance imaging of the head revealed a pituitary macroadenoma. The TSH response to TRH stimulation was normal and responses of other anterior pituitary hormones to stimulation tests were also normally preserved. Administration of octreotide with iodine successfully reversed hyperthyroidism prior to total resection of pituitary adenoma, which was followed by hemithyroidectomy of the left thyroid five months later. Histologically, the resected pituitary adenoma was a TSH-producing adenoma (TSH-oma) and the thyroid nodule was a papillary adenocarcinoma. Serum TSH diminished to undetectable levels immediately following pituitary adenomectomy but gradually normalized over nine months. Coexistence of a TSH-oma with thyroid cancer is very rare and only two similar cases have previously been documented. This combination raises the possibility that TSH may be involved in tumorigenesis in the thyroid gland.

DOI： 10.1507/endocrj.47.731

Differential effect of chronic inhibition of calcium channel and angiotensin II type 1-receptor on aldosterone synthesis in spontaneously hypertensive rats

F Otsuka, T Ogura, H Kataoka, M Kishida, M Takahashi, Y Mimura, T Yamauchi, H Makino

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 74 ( 3 ) 125 - 136 2000年10月

記述言語：英語出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

We have investigated the in vivo effect of chronic blockade of Ca2+-channels and angiotensin II type I (AT(1))-receptors on aldosterone (Aldo)-synthesis in the adrenal glands of spontaneously hypertensive rats (SHR). Male SHR were administered Ca2+-antagonist, amlodipine (10 mg/kg per day) or AT(1)-receptor-antagonist, TCV-116 (1 mg/kg per day) from 7 until 11 weeks of age. Systolic blood pressure (SBP) and heart rate (HR) were significantly higher in SHR than Wistar-Kyoto (WKY) rats. Both treatments resulted in equivalent and significant reduction in SEP in SHR. Aldo-secretion in SHR, which was significantly higher than in WKY rats, was profoundly suppressed by TCV-116 compared with amlodipine. Both treatments resulted in thickening of the tuna glomerulosa, which immunohistochemically contains Aldo, at the end of therapy. Competitive reverse transcription-polymerase chain reaction (RT-PCR) showed that CYP11A (P450scc) mRNA regulating the first step of Aldo-synthesis was significantly reduced from week 9 of age by amlodipine, and that CYP11A (P450aldo) mRNA regulating the last step of Aldo-synthesis was potently suppressed from 9 weeks of age by TCV-116. Our results indicate that chronic treatment with different antihypertensive agents directly modulates adrenocortical aldosterone synthesis in SHR in vivo via different mechanisms. (C) 2000 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S0960-0760(00)00096-0

Testosterone modulates serum leptin concentrations in a male patient with hypothalamic hypogonadism

T Ogura, K Tobe, Y Mimura, F Otsuka, T Yamauchi, A Imai, C Tsukamoto, Y Iwasaki, K Matsuura

JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION 23 ( 4 ) 246 - 250 2000年4月

記述言語：英語出版者・発行元：EDITRICE KURTIS S R L

Serial measurements of body mass index (BMI), serum concentrations of testosterone (T), estradiol (E) and leptin (L) were performed before and after gonadotropin (Gn) therapy in an 18-year-old male subject (BMI 25.4 kg/m(2)) with idiopathic hypothalamic hypogonadism (IHH). We also measured the BMI and serum concentrations of L in 99 age-matched healthy subjects. Serum L correlated significantly with BMI in control subjects (r=0.84, p<0.0001). Baseline serum concentrations of L in our case were markedly high and both T and E were very low, but On therapy resulted in a gradual decrease in L and improvement in T and E, finally reaching the control levels of BMI-matched subjects. Our results demonstrate that T is a powerful negative modulator of serum L independent of BMI in conditions associated with low T levels, such as IHH. (C) 2000, Editrice Kurtis.

Pseudomalabsorption of levothyroxine: A case report

D Ogawa, F Otsuka, Y Mimura, A Ueno, H Hashimoto, M Kishida, T Ogura, H Makino

ENDOCRINE JOURNAL 47 ( 1 ) 45 - 50 2000年2月

記述言語：英語出版者・発行元：JAPAN ENDOCRINE SOCIETY

A 51-year-old woman who had been treated with levothyroxine sodium because of hypothyroidism after total thyroidectomy for thyroidal cancer was admitted to our hospital for persistent hypothyroidism despite large dose administration of levothyroxine (600 mu g/day). The patient complained of severe general fatigue and body weight gain. Free thyroxine, free triiodothyronine and thyrotropin levels were 0.97 ng/dl, 1.55 pg/ml and 24.51 mu U/ml, respectively, under oral administration of levothyroxine. Levothyroxine loading test performed by liquid form, pulverized tablets via nasogastric tube and intravenous administration revealed no evidence of malabsorption or metabolic disorder of levothyroxine, although oral intake of tablets was ineffective due to her factitiousness. We report here a possible case of "pseudomalabsorption of levothyroxine" to emphasize the clinical recognition of this disorder in patients with resistant hypothyroidism.

Effects of angiotensin-converting enzyme inhibitor and α1-adrenoceptor antagonisit on hypertension induced in rats by long-term inhibition of nitoric oxide production.

Takahashi M, Otsuka F, Ogura T, Yamauchi T, Kataoka H, Kishida M, Mimura Y, Makino H

Clinical Experimental Nephrology 4 ( 2 ) 126 - 132 2000年

Serum leptin correlates with serum uric acid but not serum testosterone in non-obese male adolescents

T Ogura, K Matsuura, F Otsuka, A Imai, C Tsukamoto, Y Mimura, Y Iwasaki, K Tobe

RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY 107 ( 1-2 ) 55 - 64 2000年

記述言語：英語出版者・発行元：P J D PUBLICATIONS LTD

To identify the serum factors that affect circulating leptin levels, we measured the serum concentrations of leptin, testosterone (T), estradiol (E), serum alanine aminotransferase, total cholesterol and uric acid (UA) in healthy male adolescents (age, 18.3 +/- 0.1 years, n=96). We also measured body mass index (BMI), percent body fat and thickness of skin fold to assess the effect of body constitution on serum leptin level. Since serum concentration of leptin significantly correlated with BMI (r=0.820, p<0.001), we analyzed the relation-ship between leptin/BMI ratio (L/BMI) and serum parameters. Analysis of data of subjects with normal serum T level showed a significant inverse correlation between L/BMI and serum T levels (n=96, r=-0.294, p<0.005), but no such correlation was present among non-obese subjects (n=70) with BMI of +/- 20% of normal (22 kg/m(2)). There was no correlation between L/BMI and serum E level. Serum UA level significantly correlated with L/BMI in both the test group (n=96, r=0.520, p<0.001) and non-obese subjects (r=0.369, p<0.005). Stepwise multiple regression analysis showed that UA independently and significantly influenced serum leptin levels in both the test and control groups. Our results demonstrate that T weakly influences serum leptin concentration, and that UA concentrations strongly influences serum leptin in healthy male adolescents independent of their obesity level.

教育学部養護教諭養成の看護学系授業・臨床実習に対する卒業生のニーズ－フォーカス・グループ法による養護教諭対象調査より－

松島紀子, 岡田加奈子, 本田優子, 天野敦子, 福田博美, 津村直子, 芝木美沙子, 小出弥生, 三村由香里, 中村朋子, 西沢義子

日本養護教諭教育学会雑誌 3 ( 1 ) 87 - 95 2000年

Treatment with a Ca2+ channel blocker, barnidipine, reduces platelet-derived growth factor B-chain mRNA in glomeruli of spontaneously hypertensive rats

M Hashimoto, T Yamauchi, T Ogura, T Oishi, Y Mimura, F Otsuka, N Kashihara, H Makino

AMERICAN JOURNAL OF NEPHROLOGY 19 ( 5 ) 615 - 621 1999年9月

記述言語：英語出版者・発行元：KARGER

We investigated the effect of barnidipine hydrochloride, a Ca2+ chan nel blocker, on the glomerular level of mRNA expression of platelet-derived growth factor (PDGF) B-chain and transforming growth factor (TGF)-beta(1) in spontaneously hypertensive rats (SHR) with reverse transcription and polymerase chain reaction. Thirteen-week-old SHR were provided with food containing barnidipine (0.6 mg/g of food, average dose during treatment: 53 mg/kg of body mass/day) for 3 weeks. A stable reduction in systolic blood pressure relative to that of age-matched control SHR was recorded after week 1 of therapy. Although no renal histological changes were observed after 3 weeks of treatment with barnidipine, the level of expression of PDGF B-chain mRNA in glomeruli was significantly reduced relative to that in control SHR, The glomerular level of TGF-beta(1) mRNA expression was not affected by the treatment, Treatment with barnidipine significantly reduced the excretion of urinary protein, Thus, the stable reduction in systemic blood pressure by barnidipine is associated with a reduction in PDGF B-chain mRNA expression in the glomerulus and reduction in urinary protein excretion in SHR. Copyright (C) 1999 S. Karger AG, Basel.

Giant insulinoma in a patient with multiple endocrine neoplasia-type I: A case report

H Kataoka, F Otsuka, T Yamauchi, M Kishida, M Takahashi, T Tamiya, Y Mimura, T Ogura, H Makino

ENDOCRINE JOURNAL 46 ( 3 ) 429 - 435 1999年6月

記述言語：英語出版者・発行元：JAPAN ENDOCRINE SOCIETY

We report a case of giant cystic insulinoma constituting part of multiple endocrine neoplasia (MEN) type I. A 29-year-old Japanese man presented with a history of recurrent hypoglycemic attacks. Endocrine examination showed hyperinsulinemia discordant with hypoglycemia, and a giant cystic insulinoma (11 x 10 cm) located in the pancreatic tail was detected radiologically. Hyperprolactinemia due to pituitary adenoma and hyperparathyroidism due to parathyroid hyperplasia were also present. The insulinoma, prolactinoma and hyperplastic parathyroid gland were surgically removed. Fluorescent microsatellite analysis detected loss of heterozygosity (LOH) in chromosome 11q13 in DNA samples from all resected tissues but not from white blood cells. This is a rare case of MEN type I because of the giant cystic insulinoma and the evidence of common LOH detected in all MEN type I tissues.

DOI： 10.1507/endocrj.46.429

Long-term administration of adrenocorticotropin modulates the expression of IGF-I and TGF-β1 mRNAs in the rat adrenal cortex.

Otsuka F, Ogura T, Yamauchi T, Kataoka H, Kishida M, Miyatake N, Mimura Y, Kageyama J, Makino H

Growth Hormone & IGF Research 9 ( 1 ) 41 - 51 1999年2月

病院基盤の訪問看護ステーションの問題点－患者67名からの検討－

三村由香里, 槇野博史, 岡庸子, 渡辺君子, 金見一二三, 森脇和彦, 片山江里子, 藤原弘道, 定金卓爾, 佐藤能之, 林逸平, 佐藤亀弘, 小倉俊郎

岡山医学会雑誌 111 ( 3 ) 85 - 93 1999年

記述言語：日本語出版者・発行元：岡山医学会

We investigated the curent status of home nursing in our instiution, and visiting nurses' station "chime", to clarify current problems and possible improvements in home care service. A large number of the subjects were aged, and had various diseases usually involving cerebrovascular disorder. Patients requiring medical treatment comprised less than half, and it was considered that support was strong in the current home nursing siuation, particulrly. We examined the age status of persons who looked after the patients, and tce burden on women who provided care. Furthemore, problems with the prenent visit nursing syntem were evaluated by an anonymous questionnaire survey of patients and their attendants. Many of the subjects were worried or troubled about what to do about exacerbation or sudden chages of he patients' condition, the health of the caretakers, and the tight schedule of the caretakers' life. Based on the results of this questionnaire, we would like to improve the cooperation among members the visting nurse staff including doctors, and physiotherapists so that each of them may better play their roles and provide more appropriate nursing based on the wishes and needs of indvidual patients patients and their caretakers rather than those of the uniform service.

その他リンク： http://ousar.lib.okayama-u.ac.jp/13693

Effect of treatment with barnidipine, a calcium channel blocker, on platelet-derived growth factor B chain from glomerulus in spontaneously hypertensive rats.

Hashimoto M, Yamauchi T, Ogura T, Oishi T, Mimura Y, Otsuka F, Kashihara N, Makino H

Am J Nephrol 1999年

Renal AT(1) receptor: Autoradiographic localization and quantification in rat

N Asano, T Ogura, Y Mimura, M Kishida, H Kataoka, F Otsuka, T Yamauchi, H Makino

RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY 100 ( 2 ) 161 - 170 1998年5月

記述言語：英語出版者・発行元：P J D PUBLICATIONS LTD

To elucidate the precise localization of angiotensin II (Ang II) type 1 (AT(1)) receptor in the kidney, we utilized in vitro macro- and micro-autoradiography (ARG) of [H-3]-Ang II bindings to the Wistar rat kidney in the presence of L158,809, a specific non-peptide AT(1) receptor antagonist. Besides, we estimated the density of renal AT, receptors using the quantification of macro-ARG. The density of [H-3]-Ang II binding to renal tissue was concentration-dependent in both renal cortex and medulla Although the addition with 500 nM arginine vasopressin and 500 nM atrial natriuretic peptide had no effect on [H-3]-Ang II, the total binding of [H-3] Ang II completely displaced by the addition with 500 nM unlabeled Ang II or L-158,809. Macro-ARG revealed that the amount of both Ang II and AT, receptors in the renal medulla gready exceeded those in the renal cortex In the medulla, the density of these receptors was not localized on the outer medulla but was confirmed mainly to the inner medulla, especially to the inner zone and longitudinal bands. Since the density and localization of AT(1) receptors was consistent with that of total Ang II receptors, it appears that AT(1) receptors comprise most of the Ang II receptors in the kidney. Micro-ARG revealed that Ang II receptors were mainly located in the glomerulus and proximal tubules of the renal cortex, as well as on the circumferences of vessels and the vasa recta of the renal medulla. The present study established a method for ARO of AT(1) receptors in the kidney as well as a method for quantifying the macro-ARG.

Renal AT(1) receptor: Computerized quantification in spontaneously hypertensive rats and DOCA-salt rats

N Asano, T Ogura, Y Mimura, F Otsuka, M Kishida, M Hashimoto, T Yamauchi, H Makino

RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY 100 ( 2 ) 171 - 180 1998年5月

記述言語：英語出版者・発行元：P J D PUBLICATIONS LTD

To assess the involvement of angiotensin II (Ang II) in the regulation of blood pressure, we investigated the alterations of renal Ang II type 1 (AT(1)) receptors in two different models of hypertension; i.e., in spontaneously hypertensive rats (SHR) and deoxycorticosterone acetate (DOCA)-salt hypertensive rats by using the method for quantification of in vitro macroautoradiography (ARG). In the SHR model, although the number of cortical AT(1) receptors equaled that of Wistar Kyoto (WKY) rats at 4 and 12 weeks of age, the number of medullary AT(1) receptors in the 4-week-old SHR animals was significantly lower than that in age-matched WKY rats, and increased by 12 weeks of age. The renal AT(1) receptor number in DOCA-salt hypertensive rats was significantly higher than that in control rats. The amount of these receptors also increased with age in both DOCA-salt hypertensive rats and control rats. These findings indicate that the development of medullary AT(1) receptors in early stages of hypertension in the SHR model differs from that in WKY rats or DOCA-salt hypertensive rats. This suggests that renal AT(1) receptors may contribute to the hypertension seen in the SHR group. The renal AT(1) receptors appear to be up-regulated in early stages of DOCA-salt-induced hypertension as well.

In vitro Macro-and Microautoradiographic Localization of V1 and V2 Receptors in the Rat Kidney Using OPC-21268 and OPC-31260

Yukari Mimura, Toshio Ogura, Nobuhiko Hayakawa, Fumio Otsuka, Masami Hashimoto, Takayoshi Yamauchi, Hirofumi Makino, Norio Ogawa

Nephron 76 ( 3 ) 331 - 336 1997年

記述言語：英語

To elucidate the precise localization of vasopressin(VP) Vj and V2 receptors in the kidney, we utilized in vitro macroautoradiography (macro-ARG) and microautoradiography (micro-ARG) of these receptors in Wistar rat kidneys.This was done by using OPC-21268 and OPC-31260, two newly developed selective Vi (OPC-21268) and V2 (OPC-31260)receptor antagonists. For macro-ARG, 10-μm kidney sections were incubated with Tris-HCl buffer containing [3H]-VP with or without unlabeled ligand (VP, OPC-21268, orOPC-31260) at 20°C for 40 min. These sections were then loaded intoX-ray cassettes with Hyperfilm-[3H] and exposed inthe dark for 2 months. The autoradiograms were quantitativelyanalyzed by using the research analysis system RAS 1,000
the V1 and V2 receptors were quantitated by inftracting the nonspecific binding (incubatedwith OPC-21268 and OPC-31260, respectively) from the total binding. To assess a more precise localization of the V1and V2 receptors, we also investigated the micro-ARG of the renal V1 and V2 receptorsby dipping the kidney section slides used for macro-ARG into a photographic emulsion and observing the receptors under light microscopy. [3H]-VP binding to the rat kidney was completely displaced by unlabeled excess VP, but not by unlabeled angiotensin II, indicating that [3H]-VP binding was specific for VP receptors. Computerized quantification showed that V2 receptors, visualized by OPC-31260, were the predominant type of VP receptor in the kidney. Conversely, V1receptors, visualized by OPC-21268, werefewer in number. V) receptors were partly localized to the glomerulus, cortical vessels, interstitial cells, and the medullary vessels. The V2receptors localized to the collecting ducts and medullary tubules. Our findings indicated that renal V1 and V2 receptors can be detected by in vitro macro- and micro-ARG by using OPC-21268 and OPC-31260. © 1997 S.Karger AG, Basel.

DOI： 10.1159/000190200

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