研究者詳細 - 一瀬　勇規

MISC - 一瀬　勇規

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植物の病原菌に体する受容性と抵抗性

新版分子レベルからみた植物の耐病性 (細胞工学別冊)，(島本功ら編) 秀潤社 pp.74-81. 2004年

PDR-type ABC transporter and tobacco defense response against pathogen

Y Ichinose, D Schenke, M Sasabe, Y Inagaki, K Toyoda, T Shiraishi

PLANT AND CELL PHYSIOLOGY 45 S1 - S1 2004年

Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101.

Genes Genet. Syst. 78 ( 6 ) 409 - 418 2003年12月

Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response

F Taguchi, R Shimizu, R Nakajima, K Toyoda, T Shiraishi, Y Ichinose

PLANT PHYSIOLOGY AND BIOCHEMISTRY 41 ( 2 ) 165 - 174 2003年2月

記述言語：英語出版者・発行元：EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

To investigate the factor that determines incompatible interactions between Pseudomonas syringae pv. tabaci and non-host plants, an elicitor of hypersensitive reaction (HR) was partially purified from the supernatant of a nutrient-poor medium of bacterial culture by DEAE column chromatography. The major protein in the elicitor-active fractions was identified as a flagellin which is a component of flagellar filaments. The flagellins purified from P. syringae pv. tomato and glycinea, incompatible pathogens of tobacco plants, induced fragmentation of chromosomal DNA and oxidative burst accompanied by programmed cell death in tobacco (Nicotiana tabacum) Bright Yellow (BY-2) cells, but the flagellin from pv. tabaci, a compatible pathogen, did not. However, the amino acid sequences of flagellins deduced from fliC genes showed a high homology among these P. syringae pathovars. In particular, the amino acid sequences of pv. tabaci and glycinea are completely identical. However, both recombinant flagellins produced in Escherichia coli possess HR-inducing activity in BY-2 cells. These results indicate that the post-translational modification of flagellins has an important role for HR-inducing ability in tobacco cells. Furthermore, we discuss the cause of a different elicitor activity among flagellins on tobacco cells and the role of flagellins in the determining specificity. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

DOI： 10.1016/S0981-9428(02)00018-9

Web of Science

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Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea.

The Scientific Reports of The Faculty of Agriculture, Okayama University. 92, in press 2003年

Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants, DfliC and DfliD, in host tobacco plants.

J. Gen. Plant Pathol. 69 (4) 244-249. 2003年

Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants, DfliC and DfliD, in host tobacco plants.

J. Gen. Plant Pathol. 69 (4) 244-249. 2003年

Role of flagella and flagellin in plant-Pseudomonas syrignae interactions. In Japan/ Taiwan Symposium on Molecular Biology of Functional Regulation in Plant and Microbe (N. Sako, H. Yaegashi and M.-K. Yang eds.)

Showado Co. (Saga, Japan) pp. 158-166. 2003年

Phylogenetic Classification of Dof type Transcription Factors in Pea (Pisum sativum)

Naoki Nakamura, Mizuri Marutani, Shiroh Sanematsu, Kazuhiro Toyoda, Yoshi-shige Inagaki, Tomonori Shiraishi, Yuki Ichinose

Plant Biotechnology 20 ( 3 ) 247 - 252 2003年

記述言語：英語

The Dof protein family is a group of plant transcription factors carrying highly conserved 52 residues referred to as the Dof domain, and belongs to the C4 zinc-finger transcription factors. We isolated various PsDof genes by PCR using the Dof-domain nucleotide sequence of the PsDofl gene from a cDNA library of elicitor-treated pea epicotyls, and these isolated PsDof genes were then classified phylogenetically. Since the obtained genes (PsDofl to PsDofT) were scattered over various positions of the phylogenetic tree, they were expected to perform various functions as Dof-type transcription factors. From their positions in the tree, it is expected that the PsDofZ and PsDof5 genes are defense related, as is the PsDofl gene. © 2003, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.

DOI： 10.5511/plantbiotechnology.20.247

Scopus

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Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens.

J. Gen. Plant Pathol. 69 (6) In press 2003年

Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens.

J. Gen. Plant Pathol. 69 (6) In press 2003年

Role of Flagella and Flagellin in Plant - Pseudomonas syringae Interactions

Pseudomonas syringae and Related Pathogens (N. S. Iacobellis ed.) Kluwer AcademicPress (Dordrecht, The Netherland) pp. 311-318. 311 - 318 2003年

Post-translational modification of flagellin determines the specificity of HR induction.

Plant Cell Physiol. 44 (3) 342-349. 2003年

植物病原細菌のべん毛を介した植物相互作用．

一瀬勇規, 田口富美子, 稲垣善茂, 豊田和弘, 白石友紀

化学と生物 41 (8) 511-516 ( 8 ) 511 - 516 2003年

Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea.

Seki Hikaru, Marutani Mizuri, Inagaki Yoshishige, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

The Scientific Reports of The Faculty of Agriculture, Okayama University. 92, in press ( 1 ) 21 - 26 2003年

記述言語：英語出版者・発行元：岡山大学農学部

Recently, we, isolated cDNA clone, PsDof1, from clicitor-treated pea cDNA library. The putative gene product, a PsDof1, encodes DNA binding protein that specifically binds the DNA fragment containing AAAG core sequence. In this paper we report that GST-PsDof1 fusion protein specifically binds to the promoter region containing AAAG core sequence(s) of PsCHS1, one of the elicitor-inducible genes encoding chalcone synthase (CHS). Furthermore the addition of DNA fragment containing AAAG motif to the 35S minimal promoter provided the elicitor-responsibility in transient transfection assay using pea protoplasts. These results suggest that PsDof1 might be involved in defense responses by acitivating the transcription by a binding to AAAG core sequence in the promoter of the defense-related genes in pea.エリシターを処理したエンドウ上胚軸由来のRNAから作成されたcDNAライブラリーからエリシター処理により発現が増高する遺伝子候補のcDNAとしてPsDof1が単離された．大腸菌で生産されたGST-PsDof１融合タンパク質はAAAG配列をコアとするDNAに結合することが明らかにされている．本論文ではGST-PsDof１がエリシター応答性遺伝子の一つ，PsCHS1のプロモーター上のAAAGまたはCTTT配列を有する断片に特異的に結合することを明らかにした．更にAAAG配列のエリシター応答性シスエレメントとしての機能を解析するため，AAAG配列を４回繰り返したユニットをCaMV35Ｓの最小プロモーターとCATレポーター遺伝子に連結したキメラ遺伝子を構築し，エンドウプロトプラストにエレクトロポレーション法により導入した．CAT活性を指にプロモーター活性を調べたところ，AAAG配列を有するプロモーターは，エリシター処理により活性化されることが明らかとなった．これらの結果はPsDof１がエリシター応答性防御遺伝子のプロモーター上のAAAG配列に結合し，転写を活性化させる可能性を示唆している．

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Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes.

J. Gen. Plant Pathol. 69: 33-38. 2003年

Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes.

J. Gen. Plant Pathol. 69: 33-38. 2003年

The DfliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cells.

Mol. Genet. Genomics 269: 21-30. 2003年

Showado Co. (Saga, Japan) pp. 158-166. 2003年

(321)Pseudomonas syringaeの生産する過敏感反応誘導因子(5)P.syringaeの生産するflagellinのタバコに対する過敏感反応誘導特異性(平成14年度日本植物病理学会大会講演要旨)

田口富美子, 清水鈴菜, 竹内香純, 池田陽子, 稲垣善茂, 白石友紀, 一瀬勇規

日本植物病理學會報 68 ( 2 ) 242 - 243 2002年8月

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