MISC - 一瀬　勇規

Brachypodium distachyonとオオムギに対するRhizoctonia solani分離株の感染性と化学物質誘導抵抗性【JST機械翻訳】

MAHADEVAN Neranjana, FERNANDA Rozi, MATSUI Hidenori, ICHINOSE Yuki, TOYODA Kazuhiro, HISANO Hiroshi, KOUZAI Yusuke, OKAMOTO Masanori, OKAMOTO Masanori, NOUTOSHI Yoshiteru

日本植物病理学会大会プログラム・講演要旨予稿集   2024   2024年

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N-ヒドロキシピペコリン酸はオオムギおよびBrachypodium distachyonにおけるRhizoctonia solonに対する耐性を付与する【JST・京大機械翻訳】

MAHADEVAN Neranjana, KOHNO Natsuka, NAGAO Reiko, NYEIN Khin Thida, MATSUI Hidenori, YAMAMOTO Mikihiro, ICHINOSE Yuki, TOYODA Kazuhiro, KOUZAI Yusuke, HISANO Hiroshi, OKAMOTO Masanori, OKAMOTO Masanori, NOUTOSHI Yoshiteru

日本植物病理学会大会プログラム・講演要旨予稿集   2023   2023年

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ブドウ根頭がんしゅ病拮抗細菌を用いた片利共生細菌のシロイヌナズナ根圏への定着能の解析

BAO J., 松井英讓, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会報   88 ( 1 )   2022年

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タバコ野火病菌type III effector Jが標的とする宿主因子の探索

樫原沙知, 西村隆史, 中神弘史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

日本植物病理学会報   88 ( 1 )   2022年

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Rhizobium vitis VAR03-1株のブドウ根頭がんしゅ病拮抗能を司るテイロシンrhizoviticinの機能解析

土田菜月, 石井智也, 渡邉恵, 齊藤晶, BAO J., 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, 豊田敦, 松原岳大, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会報   88 ( 1 )   2022年

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MAMP応答性Raf-likeプロテインキナーゼ1(MRPK1)が介する植物免疫シグナルの解析

藤山祐香, 松井英譲, 松井英譲, HYON G.-S., 野村有子, 能年義輝, 豊田和弘, 一瀬勇規, 中神弘史, 中神弘史

日本植物病理学会報   88 ( 1 )   2022年

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ブドウ根頭がんしゅ病拮抗細菌を用いた片利共生細菌のシロイヌナズナ根圏への定着能の解析

BAO Jiyuan, HEMELDA Niarsi Merry, 土田菜月, 渡邉恵, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 豊田和弘, 豊田和弘, 一瀬勇規, 一瀬勇規, 川口章, 能年義輝, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022年

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保存され分泌されるペプチドのファミリーであるCEPペプチドはシロイヌナズナの免疫を負に調節する(4)CEPペプチドはサリチル酸を介した免疫を抑制する

長谷川晴香, 伊藤千晶, FITRIANTI Aprilia Nur, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 能年義輝, 能年義輝, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

植物微生物研究会研究交流会講演要旨集   31st   2022年

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保存され分泌されるペプチドのファミリーであるCEPペプチドはシロイヌナズナの免疫を負に調節する(3)CEPペプチドはPTIおよびETIを抑制する

伊藤千晶, 長谷川晴香, FITRIANTI Aprilia Nur, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 能年義輝, 能年義輝, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

植物微生物研究会研究交流会講演要旨集   31st   2022年

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非親和性および不適応型病原細菌を接種したシロイヌナズナ葉におけるCEP遺伝子の応答

APRILIA Nur Fitrianti, 伊藤千晶, 長谷川晴香, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022年

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タバコ野火病菌のType IIIエフェクターが標的とする宿主因子の網羅的同定にむけて

黒江香那, 樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 中神弘史, 一瀬勇規, 松井英譲

日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022年

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タバコ野火病菌のeffector Jの機能解析

樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 中神弘史, 一瀬勇規, 松井英譲

日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022年

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タバコ野火病菌の病徴進展に寄与するエフェクターの同定

西村隆史, 樫原沙知, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

日本植物病理学会報   87 ( 1 )   2021年

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シロイヌナズナのMAMP誘導性オキシダティブバーストにおける細胞壁ペルオキシダーゼとNADPHオキシダーゼの関与

木元菜々子, 高須瑞穂, APRILIA Nur Fitrianti, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報   87 ( 1 )   2021年

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シロイヌナズナの細胞内/細胞外レドックス制御におけるNADPHオキシダーゼRBOHDの役割

高須瑞穂, 木元菜々子, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報   87 ( 1 )   2021年

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シロイヌナズナにおける既知の受容体を介さないカビの認識機構

矢野裕奈, APRILIA Nur Fitrianti, 木元菜々子, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報   87 ( 1 )   2021年

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メトミノストロビンの抵抗性誘導作用に関する研究 5.シロイヌナズナのPTI応答におけるMAPキナーゼ活性に対する影響

伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021年

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比較ゲノム解析によるPseudomonas syringae pv.tabaciの病原力因子の探索

吉岡桂, 西村隆史, 浅井秀太, 増田幸子, 白須賢, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規, 松井英譲

日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021年

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タバコ野火病抵抗性品種はタバコ野火病菌TTSSエフェクターを認識し,HRを誘導する

樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

日本植物病理学会報   87 ( 1 )   2021年

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メトミノストロビンの抵抗性誘導作用に関する研究(3)メトミノストロビンはシロイヌナズナのMAPキナーゼを活性化する

伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本農薬学会大会講演要旨集   46th (CD-ROM)   2021年

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メトミノストロビンの抵抗性誘導作用に関する研究 6.シロイヌナズナにおけるPTI関連遺伝子の発現に対する影響

小原七海, 伊藤千晶, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021年

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Pseudomonas syringae pv.tabaci 6605の走化性受容体遺伝子mcpの病原力への関与

寺元茜, 金重慎, TUMEWU S.A., 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021年

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MARK2のリン酸化はflg22処理に伴うカロース蓄積に関与する

松井英譲, 松井英譲, 澁谷日奈, 前田和輝, 四井いずみ, 四井いずみ, HYON G-S., 野村有子, 一瀬勇規, 中神弘史, 中神弘史

日本植物病理学会報   87 ( 1 )   2021年

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メトミノストロビンの抵抗性誘導作用に関する研究(4)メトミノストロビンはシロイヌナズナにおいてPTI関連遺伝子の発現を促進する

小原七海, 伊藤千晶, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本農薬学会大会講演要旨集   46th (CD-ROM)   2021年

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シロイヌナズナにおけるオキシダティブバースト反応の前提条件:細胞内/細胞外レドックス制御におけるNADPHオキシダーゼRBOHDの役割

高須瑞穂, 木元菜々子, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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タバコ野火病抵抗性品種が認識するエフェクターの探索

樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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mark2欠損変異体はPto接種時のジャスモン酸関連遺伝子発現が低下する

澁谷日奈, 前田和輝, 四井いずみ, 四井いずみ, HYON G-S., 野村有子, 一瀬勇規, 松井英譲, 松井英譲, 中神弘史, 中神弘史

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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イネ科モデル植物ミナトカモジグサの微生物分子パターン応答性の解析

小笠原翼, 香西雄介, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 持田恵一, 能年義輝

日本植物病理学会報   86 ( 1 )   2020年

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Pseudomonas syringae pv.tabaciの病原力因子の探索

西村隆史, 樫原沙知, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規, 松井英譲

日本植物病理学会報   86 ( 1 )   2020年

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シロイヌナズナの分泌型ペルオキシダーゼPRX34はMAMP誘導性オキシダティブバーストに関与する

高須瑞穂, 木元菜々子, LEI Zhao, PHUONG Le Thi, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報   86 ( 1 )   2020年

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タバコ野火病菌の病徴進展に寄与するエフェクターの同定

西村隆史, 樫原沙知, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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タバコ野火病菌のc-di-GMP related gene1は病原力に寄与する

澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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シロイヌナズナのMAMP誘導性オキシダティブバースト反応に対する細胞壁ペルオキシダーゼとNADPHオキシダーゼの寄与

木元菜々子, 高須瑞穂, APRILIA NUR F., ZHAO L., LE THI P., 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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Rhizobium vitis VAR03-1株のブドウ根頭がんしゅ病菌に対する拮抗能を司るテイロシンの解析

石井智也, 齋藤晶, 渡邉恵, 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, BAO J., 松井英譲, 山本幹博, 豊田和弘, 一瀬勇規, 川口章, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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シロイヌナズナにおける既知の受容体を必要としない病原糸状菌の認識

矢野裕奈, APRILIA NUR F., 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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メトミノストロビンの作用機構に関する研究 II.コムギにおけるうどんこ病抵抗性のプライミング

佐藤穂高, PHUONG Le Thi, 市成光広, 櫻本和生, 山田晶, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報   86 ( 1 )   2020年

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抵抗性誘導剤のシーズとして単離したサリチル酸配糖化酵素阻害剤の特性解析

篠原優佳, 渡邉恵, 楠和輝, 谷川友里佳, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 熊谷和夫, 米須清明, 能年義輝

日本植物病理学会報   86 ( 1 )   2020年

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Brachypodium distachyonにおけるRhizoctonia solaniのエフェクター遺伝子の発現プロファイルの特性化【JST・京大機械翻訳】

ABDELSALAM S. S. H., ISOYA S., WATANABE M., KOUZAI Y., MATSUI H., YAMAMOTO M., ICHINOSE Y., TOYODA K., MOCHIDA K., TSUGE S., NOUTOSHI Y.

日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

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抵抗性誘導剤のシーズとして単離したサリチル酸配糖化酵素阻害剤の反応速度論解析

篠原優佳, 渡邉恵, 楠和輝, 谷川友里佳, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 熊谷和夫, 米須清明, 能年義輝

日本植物病理学会報   85 ( 1 )   2019年

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植物免疫活性剤インプリマチンDがpattern-triggered immunityに与える影響

山枡一秀, 平田奈美, 福井絢子, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019年

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MAMP応答性タンパク質MARK2の病原菌感染時の機能解析

澁谷日奈, 前田和輝, 四井いずみ, 四井いずみ, HYON G-S., 野村有子, 一瀬勇規, 一瀬勇規, 松井英譲, 松井英譲, 松井英譲, 中神弘史, 中神弘史

日本植物病理学会報   85 ( 1 )   2019年

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微生物分子パターンに対するミナトカモジグサの活性酸素種生成特性の解析

小笠原翼, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 香西雄介, 能年義輝

日本植物病理学会報   85 ( 1 )   2019年

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Pseudomonas syringae pv.tabaciのRNAタイプ多剤排出トランスポーターの病原力における機能解析

西村隆史, 一瀬勇規, 原田みのり, 松井英譲, 山本幹博, 能年義輝, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019年

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エンドウの細胞壁画分に見出されたキトサンと結合するタンパク質複合体

松尾実佳, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019年

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エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質

松尾実佳, 川端真矢, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報   85 ( 1 )   2019年

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非病原性Rhizobium vitis VAR03-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制機構の解析

石井智也, 渡邉恵, 齊藤晶, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会報   85 ( 1 )   2019年

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タバコ野火病菌のCDG1はアシルホモセリンラクトンの蓄積を正に制御する

澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

日本植物病理学会報   85 ( 1 )   2019年

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メトミノストロビンの作用機構に関する研究

佐藤穂高, 市成光広, 櫻本和生, 山田晶, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報   85 ( 1 )   2019年

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細胞外オキシダティブバースト反応の分子機構:ペルオキシダーゼのスルフェン酸化とその役割

高須瑞穂, 吉岡美樹, 吉岡博文, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報   85 ( 1 )   2019年

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Pseudomonas syringaeの菌体密度感知機構の解析(4)菌体密度に応じた遺伝子発現制御

中津有紀子, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018年

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細菌シグナル伝達因子CDG proteinの機能解析

澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018年

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MAMP応答性リン酸化タンパク質MARK2の機能解析

澁谷日奈, 前田和輝, 四井いずみ, 四井いずみ, HYON G.-S., 野村有子, 一瀬勇規, 松井英譲, 松井英譲, 中神弘史, 中神弘史

日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018年

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Pseudomonas syringaeの菌体密度感知機構の解析(2)

一瀬勇規, 松井英譲, 石賀康博, 山本幹博, 能年義輝, 豊田和弘

日本植物病理学会報   84 ( 1 )   2018年

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非病原性Rhizobium vitis VAR03-1株の培養上清がブドウ根頭がんしゅ病菌に及ぼす影響

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会報   84 ( 1 )   2018年

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ササゲにおける細胞外オキシダティブバースト反応の分子機構

川端真矢, 佐藤穂高, 高須瑞穂, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報   84 ( 1 )   2018年

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エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質

松尾実佳, 川端真矢, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

植物微生物研究会研究交流会講演要旨集   28th   2018年

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Pseudomonas syringaeの菌体密度感知機構の解析(3)PsyRとアシルホモセリンラクトンによる遺伝子発現制御機構

田阪洋昌, 山本悟, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018年

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バイオコントロール細菌Rhizobium vitis VAR03-1株のブドウ根頭がんしゅ病抑制機構の解析

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018年

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Pseudomonas syringaeの菌体密度感知機構の解析(1)

山本悟, 藤山友里, 高田基弘, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会報   83 ( 1 )   2017年

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非病原性Rhizobium vitis ARK-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会報   83 ( 1 )   2017年

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エクト型ATPase(PsAPY1)はエンドウ葉におけるPAMP誘導性オキシダティブバースト反応を正に調節し,不適応型の病原菌に対する非宿主抵抗性に関与する

山崎史織, 三木紅葉, 矢尾幸世, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報   83 ( 1 )   2017年

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ミナトカモジグサにおける植物免疫ホルモン応答性マーカー遺伝子の同定と発現プロファイルの解析

香西雄介, 山中由利恵, 渡邉恵, 木村麻美子, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 恩田義彦, 持田恵一, 能年義輝

日本植物病理学会報   83 ( 1 )   2017年

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シロイヌナズナにおける細胞外オキシダティブバースト反応の分子機構

松尾実佳, 片岡千香子, 山崎史織, 三木紅葉, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報   83 ( 1 )   2017年

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シロイヌナズナにおけるPAMP誘導性オキシダティブバースト反応におけるアポプラストの役割について

片岡千香子, 山崎史織, 松尾実佳, 三木紅葉, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報   83 ( 1 )   2017年

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Pseudomonas syringae pv.tabaci6605におけるAHL合成制御機構並びにMexEFOprN多剤排出ポンプの病原力における役割の解析

澤田貴博, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会報   83 ( 1 )   2017年

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エンドウから分離した細胞壁タンパク質のPAMP応答

三木紅葉, 山崎史織, 片岡千香子, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報   83 ( 1 )   2017年

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エンドウならびにシロイヌナズナにおけるPAMP誘導性細胞外オキシダティブバースト反応の分子機構

片岡千香子, 三木紅葉, 山崎史織, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2017   2017年

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非病原性Rhizobium vitis VAR03-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集   2017   2017年

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エクト型ATPase(PsAPY1)はエンドウ葉におけるリポ多糖(LPS)誘導性細胞外オキシダティブバースト反応を正に調節する

三木紅葉, 山崎史織, 矢尾幸世, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016年

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Pseudomonas syringae pv.syringae B728aの病原力制御因子Vfrの標的遺伝子のクロマチン免疫沈降法による探索(2)

小倉敬右, 田口富美子, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016年

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エクト型ATPase(PsAPY1)の発現を抑制したエンドウ葉におけるジャスモン酸応答性遺伝子の発現

矢尾幸世, 田中佳織, 奥田竜太, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘

日本植物病理学会報   82 ( 1 )   2016年

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Pseudomonas syringae pv.tabaciにおけるAHL合成制御機構の解析

澤田貴博, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会報   82 ( 1 )   2016年

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ミナトカモジグサ-紋枯病菌感染系の確立と植物ホルモンによる抵抗性機構の解析

香西雄介, 山中由利恵, 渡邉恵, 木村麻美子, 恩田義彦, 持田恵一, 山本幹博, 松井英譲, 一瀬勇規, 豊田和弘, 能年義輝

日本植物病理学会報   82 ( 1 )   2016年

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非病原性Rhizobium vitis ARK-1株によるブドウ根頭がんしゅ病の発病発現抑制機構の解析

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016年

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ナス科植物青枯病菌の病原力や増殖を制御する化合物の探索

澤井拓, 一瀬勇規, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 向原隆文, 長田裕之

日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016年

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サリチル酸配糖化酵素を用いた抵抗性誘導剤の標的ベース探索

谷川友里佳, 渡邉恵, 熊谷和夫, 香西雄介, 山中由利恵, 木村麻美子, 稲垣善茂, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

日本植物病理学会報   81 ( 1 )   2015年

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植物免疫プライミング剤インプリマチンDの作用解析

福井絢子, 香西雄介, 稲垣善茂, 山本幹博, 一瀬勇規, 豊田和弘, 白須賢, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集   2015   2015年

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Pathogenicity and virulence factors of Pseudomonas syringae

Yuki Ichinose, Fumiko Taguchi, Takafumi Mukaihara

Journal of General Plant Pathology   79 ( 5 )   285 - 296   2013年9月

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記述言語：英語   掲載種別：書評論文，書評，文献紹介等  

In 1994, Oku reported that plant pathogens, mainly fungal pathogens, require three essential abilities to infect plants: to enter plants, to overcome host resistance, and to evoke disease. Because the infectious process of phytopathogenic bacteria differs from that of fungal pathogens, we have attempted to characterize pathogenicity, the ability of a pathogen to cause disease, using the phytopathogenic bacterium Pseudomonas syringae as a representative pathogen. To establish infection and incite disease development, bacteria first have to enter a plant. This process requires flagella- and type IV pili-mediated motility, and active taxis is probably necessary for effective infection. After bacteria enter a plant's apoplastic spaces, they need to overcome host plant resistance. To do this, they secrete a wide variety of hypersensitive response and pathogenicity (Hrp) effector proteins into the plant cytoplasm to interfere with pathogen/microbe-associated molecular pattern- and effector-triggered immunity, produce phytohormones and/or phytotoxins to suppress plant defense responses and extracellular polysaccharides to prevent access by antibiotics and to chelate Ca2+, and activate the multidrug resistance efflux pump to extrude antimicrobial compounds for successful colonization. Furthermore, to evoke disease, bacteria produce toxins and Hrp effectors that compromise a plant's homeostasis and injure plant cells. The expression of these virulence factors depends on the infection processes and environmental conditions. Thus, the expression and function of virulence factors interact with each other, creating complex networks in the regulation of bacterial virulence-related genes. © 2013 The Phytopathological Society of Japan and Springer Japan.

DOI： 10.1007/s10327-013-0452-8

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A volatile substance from Talaromyces sp promotes the plant growth and blocks the disease development on several plants

T. Shiraishi, Y. Yamagiwa, P. T. Le, K. Maeda, Y. Inagaki, Y. Ichinose, M. Hyakumachi, K. Toyoda

PHYTOPATHOLOGY   101 ( 6 )   S165 - S166   2011年6月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER PHYTOPATHOLOGICAL SOC  

Web of Science

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立枯病・青枯病抵抗性誘導物質の単離同定と作用機構の解析

瀬尾茂美, 五味剣二, 加来久敏, 中保一浩, 小林光智衣, 瀬戸秀春, 安部洋, 一瀬勇規, 光原一朗, 大橋祐子

日本植物生理学会年会要旨集   52nd   222   2011年3月

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記述言語：日本語  

DOI： 10.14841/jspp.2011.0.0443.0

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Siderophore pyoverdine of Pseudomonas syringae pv. tabaci 6605 is an intrinsic virulence factor on host tobacco infection. 査読

Taguchi, F, Suzuki, T, Inagaki, Y, Toyoda, K, Shiraishi, T, Ichinose, Y

J. Bacteriol.   192 ( 1 )   117 - 126   2010年1月

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担当区分：最終著者,　責任著者   記述言語：英語  

DOI： 10.1128/jb.00689-09

Web of Science

PubMed

CiNii Article

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Bacterial DNA activates immunity in Arabidopsis thaliana

Suguru Yakushiji, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

JOURNAL OF GENERAL PLANT PATHOLOGY   75 ( 3 )   227 - 234   2009年6月

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記述言語：英語   出版者・発行元：SPRINGER TOKYO  

To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that non-methylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.

DOI： 10.1007/s10327-009-0162-4

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Bacterial DNA activates immunity in Arabidopsis thaliana

Suguru Yakushiji, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Journal of General Plant Pathology   75 ( 3 )   227 - 234   2009年6月

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担当区分：最終著者,　責任著者   記述言語：英語   出版者・発行元：Springer Science and Business Media LLC  

To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that non-methylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.

DOI： 10.1007/s10327-009-0162-4

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その他リンク： http://link.springer.com/article/10.1007/s10327-009-0162-4/fulltext.html

Enhanced Defense Responses in Arabidopsis Induced by the Cell Wall Protein Fractions from Pythium oligandrum Require SGT1, RAR1, NPR1 and JAR1

Yoko Kawamura, Shigehito Takenaka, Shu Hase, Mayumi Kubota, Yuki Ichinose, Yoshinori Kanayama, Kazuhiro Nakaho, Daniel F. Klessig, Hideki Takahashi

Plant and Cell Physiology   50 ( 5 )   924 - 934   2009年5月

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記述言語：英語   出版者・発行元：Oxford University Press (OUP)  

The cell wall protein fraction (CWP) is purified from the non-pathogenic biocontrol agent Pythium oligandrum and is composed of two glycoproteins (POD-1 and POD-2), which are structurally similar to class III elicitins. In tomato plants treated with CWP, jasmonic acid (JA)- and ethylene (ET)-dependent signaling pathways are activated, and resistance to Ralstonia solanaceraum is enhanced. To dissect CWP-induced defense mechanisms, we investigated defense gene expression and resistance to bacterial pathogens in Arabidopsis thaliana ecotype Col-0 treated with CWP. When the leaves of Col-0 were infiltrated with CWP, neither visible necrosis nor salicylic acid (SA)-responsive gene (PR-1 and PR-5) expression was induced. In contrast, JA-responsive gene (PDF1.2 and JR2) expression was up-regulated and the resistance to R. solanaceraum and Pseudomonas syringae pv. tomato DC3000 was enhanced in response to CWP. Such CWP-induced defense responses were completely compromised in CWP-treated coi1-1 and jar1-1 mutants with an impaired JA signaling pathway. The induction of defense-related gene expression after CWP treatment was partially compromised in ET-insensitive ein2-1 mutants, but not in SA signaling mutants or nahG transgenic plants. Global gene expression analysis using cDNA array also suggested that several other JA- and ET-responsive genes, but not SA-responsive genes, were up-regulated in response to CWP. Further analysis of CWP-induced defense responses using another eight mutants with impaired defense signaling pathways indicated that, interestingly, the induction of JA-responsive gene expression and enhanced resistance to two bacterial pathogens in response to CWP were completely compromised in rar1-1, rar1-21, sgt1a-1, sgt1b (edm1) and npr1-1 mutants. Thus, the CWP-induced defense system appears to be regulated by JA-mediated and SGT1-, RAR1- and NPR1-dependent signaling pathways.

DOI： 10.1093/pcp/pcp044

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Enhanced Defense Responses in Arabidopsis Induced by the Cell Wall Protein Fractions from Pythium oligandrum Require SGT1, RAR1, NPR1 and JAR1

Yoko Kawamura, Shigehito Takenaka, Shu Hase, Mayumi Kubota, Yuki Ichinose, Yoshinori Kanayama, Kazuhiro Nakaho, Daniel F. Klessig, Hideki Takahashi

PLANT AND CELL PHYSIOLOGY   50 ( 5 )   924 - 934   2009年5月

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記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

The cell wall protein fraction (CWP) is purified from the non-pathogenic biocontrol agent Pythium oligandrum and is composed of two glycoproteins (POD-1 and POD-2), which are structurally similar to class III elicitins. In tomato plants treated with CWP, jasmonic acid (JA)- and ethylene (ET)-dependent signaling pathways are activated, and resistance to Ralstonia solanaceraum is enhanced. To dissect CWP-induced defense mechanisms, we investigated defense gene expression and resistance to bacterial pathogens in Arabidopsis thaliana ecotype Col-0 treated with CWP. When the leaves of Col-0 were infiltrated with CWP, neither visible necrosis nor salicylic acid (SA)-responsive gene (PR-1 and PR-5) expression was induced. In contrast, JA-responsive gene (PDF1.2 and JR2) expression was up-regulated and the resistance to R. solanaceraum and Pseudomonas syringae pv. tomato DC3000 was enhanced in response to CWP. Such CWP-induced defense responses were completely compromised in CWP-treated coi1-1 and jar1-1 mutants with an impaired JA signaling pathway. The induction of defense-related gene expression after CWP treatment was partially compromised in ET-insensitive ein2-1 mutants, but not in SA signaling mutants or nahG transgenic plants. Global gene expression analysis using cDNA array also suggested that several other JA- and ET-responsive genes, but not SA-responsive genes, were up-regulated in response to CWP. Further analysis of CWP-induced defense responses using another eight mutants with impaired defense signaling pathways indicated that, interestingly, the induction of JA-responsive gene expression and enhanced resistance to two bacterial pathogens in response to CWP were completely compromised in rar1-1, rar1-21, sgt1a-1, sgt1b (edm1) and npr1-1 mutants. Thus, the CWP-induced defense system appears to be regulated by JA-mediated and SGT1-, RAR1- and NPR1-dependent signaling pathways.

DOI： 10.1093/pcp/pcp044

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High level expression of a virus resistance gene, RCY1, confers extreme resistance to Cucumber Mosaic Virus in Arabidopsis thaliana.

21 ( 11 )   1398 - 1407   2008年11月

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記述言語：英語  

DOI： 10.1094/MPMI-21-11-1398

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High Level Expression of a Virus Resistance Gene, RCY1, Confers Extreme Resistance to Cucumber mosaic virus in Arabidopsis thaliana

Ken-Taro Sekine, Sayaka Kawakami, Shu Hase, Mayumi Kubota, Yuki Ichinose, Jyoti Shah, Hong-Gu Kang, Daniel F. Klessig, Hideki Takahashi

MOLECULAR PLANT-MICROBE INTERACTIONS   21 ( 11 )   1398 - 1407   2008年11月

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記述言語：英語   出版者・発行元：AMER PHYTOPATHOLOGICAL SOC  

A coiled coil-nucleotide binding site-leucine rich repeat-type resistance gene, RCY1, confers resistance to a yellow strain of Cucumber mosaic virus, CMV(Y), in Arabidopsis thaliana ecotype C24. Resistance to CMV(Y) in C24 is accompanied by a hypersensitive response (HR) that is characterized by the development of necrotic local lesions at the primary infection sites. To further study the HR and resistance to CMV(Y) in ecotype Col-0, which is susceptible to CMV(Y), Col-0 were transformed with RCY1. Systemic spread of CMV(Y) was completely suppressed in RCY1-transformed Col-0 (Col::pRCY1 lines 2 to 6), whereas virulent strain CMV(B2) spread and multiplied systemically in these transgenic lines similar to that in wild-type Col-0. Interestingly, the resistant phenotype of Col:pRCY1 varied among the lines. In lines 3 and 6, in which levels of RCY1 transcript were similar to that in wild-type C24, the HR and resistance to CMV(Y) was induced. Line 4, which expresses moderately elevated levels of RCY1 transcript, exhibited moderately enhanced resistance compared with that in C24 or line 3. In contrast, lines 2 and 5, which highly overexpress the RCY1 gene, did not exhibit either visible lesions or a micro-HR on the inoculated leaves. Moreover, virus coat protein was not detected in either inoculated or noninoculated upper leaves of these two lines, suggesting that extreme resistance (ER) to CMV(Y) was induced by high levels of expression of RCY1. Furthermore, in transgenic lines expressing hemagglutinin (HA) epitope-tagged RCY1 (Col::pRCY1-HA), high levels of accumulation of RCY1-HA protein were also correlated with the ER phenotype. Global gene expression analysis in line 2, which highly overexpresses RCY1, indicated that expression of several defense-related genes were constitutively elevated compared with wild-type Col-0. Despite this, line 2 did not have enhanced resistance to other avirulent and virulent pathogens. Take together, constitutive accumulation of high levels of RCY1 protein appears to regulate the strength of RCY1-conferred resistance in a gene-for-gene manner and implies that ER and HR-associated resistance differ only in the strength of resistance.

DOI： 10.1094/MPMI-21-11-1398

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Biological Control of Crown Gall of Grapevine, Rose, and Tomato by Nonpathogenic Agrobacterium vitis Strain VAR03-1

A. Kawaguchi, K. Inoue, Y. Ichinose

PHYTOPATHOLOGY   98 ( 11 )   1218 - 1225   2008年11月

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担当区分：最終著者   記述言語：英語   出版者・発行元：AMER PHYTOPATHOLOGICAL SOC  

A nonpathogenic strain of Agrobacterium vitis VAR03-1 was tested as a biological control agent for crown gall of grapevine (Vitis vinifera). When roots of grapevine, rose (Rose multiflora), and tomato (Lycopersicon esculentum) were soaked in a cell suspension of antagonists before planting in soil infested with tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens, respectively, treatment with VAR03-1 significantly reduced the number of plants with tumors and disease severity in the three plant species. The inhibitory effects of treatment with VAR03-1 and the nonpathogenic A. rhizogenes strain K84 on crown gall of rose and tomato were almost identical, and the inhibitory effect of VAR03-1 on grapevine was superior to that of K84. Moreover, VAR03-1 greatly controlled crown gall of grapevine due to tumorigenic A. vitis in the field. VAR03-1 established populations averaging 10(6) colony forming units (CFU)/g of root in the rhizosphere of grapevine and persisted on roots for 2 years. VAR03-1 was bacteriocinogenic, producing a halo of inhibition against those three species of Agrobacterium. This is the first report that a nonpathogenic strain, VAR03-1, can effectively control crown gall caused by tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens.

DOI： 10.1094/PHYTO-98-11-1218

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Biological Control of Crown Gall of Grapevine, Rose, and Tomato by Nonpathogenic Agrobacterium vitis Strain VAR03-1

A. Kawaguchi, K. Inoue, Y. Ichinose

PHYTOPATHOLOGY   98 ( 11 )   1218 - 1225   2008年11月

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記述言語：英語   出版者・発行元：AMER PHYTOPATHOLOGICAL SOC  

A nonpathogenic strain of Agrobacterium vitis VAR03-1 was tested as a biological control agent for crown gall of grapevine (Vitis vinifera). When roots of grapevine, rose (Rose multiflora), and tomato (Lycopersicon esculentum) were soaked in a cell suspension of antagonists before planting in soil infested with tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens, respectively, treatment with VAR03-1 significantly reduced the number of plants with tumors and disease severity in the three plant species. The inhibitory effects of treatment with VAR03-1 and the nonpathogenic A. rhizogenes strain K84 on crown gall of rose and tomato were almost identical, and the inhibitory effect of VAR03-1 on grapevine was superior to that of K84. Moreover, VAR03-1 greatly controlled crown gall of grapevine due to tumorigenic A. vitis in the field. VAR03-1 established populations averaging 10(6) colony forming units (CFU)/g of root in the rhizosphere of grapevine and persisted on roots for 2 years. VAR03-1 was bacteriocinogenic, producing a halo of inhibition against those three species of Agrobacterium. This is the first report that a nonpathogenic strain, VAR03-1, can effectively control crown gall caused by tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens.

DOI： 10.1094/PHYTO-98-11-1218

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Amino Acid Sequence of Bacterial Microbe-Associated Molecular Pattern flg22 Is Required for Virulence 国際誌

Kana Naito, Fumiko Taguchi, Tomoko Suzuki, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

MOLECULAR PLANT-MICROBE INTERACTIONS   21 ( 9 )   1165 - 1174   2008年9月

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担当区分：最終著者,　責任著者   記述言語：英語   出版者・発行元：AMER PHYTOPATHOLOGICAL SOC  

Flagellin proteins derived from Pseudomonas syringae pv. tabaci 6605 and flg22(Pa) (QRLSTGSRINSAKDDAAGLQIA), one of the microbe-associated molecular patterns (MAMP) in bacterial flagellin, induce cell death and growth inhibition in Arabidopsis thaliana. To examine the importance of aspartic acid (D) at position 43 from the N-terminus of a flagellin in its elicitor activity, D43 was replaced with valine (V) and alanine (A) in P. syringae pv. tabaci flagellin and flg22(Pta). The abilities of flagellins from P syringae pv. tabaci D43V and D43A to induce cell death and growth inhibition were reduced, whereas the abilities of flg22(Pta)D43V and flg22(Pta)D43A were abolished. These results indicate that D43 is important for elicitor activity in P. syringae pv. tabaci. When tobacco plants were inoculated with each bacterium by the spray method, both P. syringae pv. tabaci D43V and D43A mutants had remarkably reduced ability to cause disease symptoms. Both mutants had reduced or no swimming and swarming motilities and adhesion ability. In P. syringae pv. tabaci D43V, little flagellin protein was detected and few flagella were observed by electron microscopy. These results indicate that mutant flagella are unstable and that flagellar motility is impaired. Thus, the amino acid residue required for MAMP activity is important for the intrinsic flagellar function.

DOI： 10.1094/MPMI-21-9-1165

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Amino Acid Sequence of Bacterial Microbe-Associated Molecular Pattern flg22 Is Required for Virulence

Kana Naito, Fumiko Taguchi, Tomoko Suzuki, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

MOLECULAR PLANT-MICROBE INTERACTIONS   21 ( 9 )   1165 - 1174   2008年9月

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記述言語：英語   出版者・発行元：AMER PHYTOPATHOLOGICAL SOC  

Flagellin proteins derived from Pseudomonas syringae pv. tabaci 6605 and flg22(Pa) (QRLSTGSRINSAKDDAAGLQIA), one of the microbe-associated molecular patterns (MAMP) in bacterial flagellin, induce cell death and growth inhibition in Arabidopsis thaliana. To examine the importance of aspartic acid (D) at position 43 from the N-terminus of a flagellin in its elicitor activity, D43 was replaced with valine (V) and alanine (A) in P. syringae pv. tabaci flagellin and flg22(Pta). The abilities of flagellins from P syringae pv. tabaci D43V and D43A to induce cell death and growth inhibition were reduced, whereas the abilities of flg22(Pta)D43V and flg22(Pta)D43A were abolished. These results indicate that D43 is important for elicitor activity in P. syringae pv. tabaci. When tobacco plants were inoculated with each bacterium by the spray method, both P. syringae pv. tabaci D43V and D43A mutants had remarkably reduced ability to cause disease symptoms. Both mutants had reduced or no swimming and swarming motilities and adhesion ability. In P. syringae pv. tabaci D43V, little flagellin protein was detected and few flagella were observed by electron microscopy. These results indicate that mutant flagella are unstable and that flagellar motility is impaired. Thus, the amino acid residue required for MAMP activity is important for the intrinsic flagellar function.

DOI： 10.1094/MPMI-21-9-1165

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(129) サポニン生合成に関与するイネOxidosqualene Cyclase遺伝子の分子解析(平成20年度日本植物病理学会大会講演要旨)

稲垣 善茂, 今岡 敦子, 藤田 景子, 荒瀬 栄, 豊田 和弘, 白石 友紀, 一瀬 勇規, Osbourn Anne

日本植物病理學會報   74 ( 3 )   201 - 201   2008年8月

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記述言語：日本語   出版者・発行元：日本植物病理学会  

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(308) Pseudomonas syringaeのフラジェリン糖鎖修飾構造とその合成に関わる遺伝子の機能解析 I(平成20年度日本植物病理学会大会講演要旨)

常見 和彦, 田口 富美子, 竹内 香純, 石井 忠, 吉田 充, 小野 裕嗣, 亀山 眞由美, 一瀬 勇規

日本植物病理學會報   74 ( 3 )   249 - 249   2008年8月

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記述言語：日本語   出版者・発行元：日本植物病理学会  

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(127) Medicago truncatula アピラーゼMtAPY1;1の機能解析(平成20年度日本植物病理学会大会講演要旨)

塩原 泰樹, 藤田 景子, 稲垣 善茂, 一瀬 勇規, 豊田 和弘, 白石 友紀

日本植物病理學會報   74 ( 3 )   200 - 200   2008年8月

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記述言語：日本語   出版者・発行元：日本植物病理学会  

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Phylogenetic and serological analyses reveal genetic diversity of Agrobacterium vitis strains in Japan

A. Kawaguchi, H. Sawada, Y. Ichinose

PLANT PATHOLOGY   57 ( 4 )   747 - 753   2008年8月

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担当区分：最終著者   記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Twenty-eight strains of Agrobacterium vitis, including both tumorigenic and nonpathogenic phenotypes involving 26 isolated in Japan and strains NCPPB 3554(T) and NCPPB 2562 isolated in Australia and Greece, respectively, were characterized by means of a slide agglutination test (SAT) using antisera prepared against somatic antigens. Phylogenetic analyses were also carried out, using the results of repetitive sequence-based polymerase chain reaction and the partial nucleotide sequences of pyrG, recA and rpoD. The A. vitis strains separated into four serogroups (A to D) based on the SAT reactivity. The phylogenetic analyses showed A. vitis strains separated into four tumorigenic groups (A to D) and one nonpathogenic group (E). Serogroups A to C corresponded exactly to genetic groups A to C, respectively, whereas serogroup D further divided into two distinct genetic groups, D and E. Genetic group E was isolated in Okayama Prefecture, Japan, and all strains of it have been found to coexist with tumorigenic strains belonging to the other groups within the same galled grapevine tissues. These results suggest that A. vitis strains are genetically heterogeneous and can be separated into several genetic groups. The differences between the nucleotide sequences of pyrG, recA and rpoD of the genetic groups will enable the development of a simple and convenient monitoring method, which will increase understanding of the dynamics of each genetic group of A. vitis in the natural environment.

DOI： 10.1111/j.1365-3059.2008.01829.x

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Phylogenetic and serological analyses reveal genetic diversity of Agrobacterium vitis strains in Japan

A. Kawaguchi, H. Sawada, Y. Ichinose

PLANT PATHOLOGY   57 ( 4 )   747 - 753   2008年8月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Twenty-eight strains of Agrobacterium vitis, including both tumorigenic and nonpathogenic phenotypes involving 26 isolated in Japan and strains NCPPB 3554(T) and NCPPB 2562 isolated in Australia and Greece, respectively, were characterized by means of a slide agglutination test (SAT) using antisera prepared against somatic antigens. Phylogenetic analyses were also carried out, using the results of repetitive sequence-based polymerase chain reaction and the partial nucleotide sequences of pyrG, recA and rpoD. The A. vitis strains separated into four serogroups (A to D) based on the SAT reactivity. The phylogenetic analyses showed A. vitis strains separated into four tumorigenic groups (A to D) and one nonpathogenic group (E). Serogroups A to C corresponded exactly to genetic groups A to C, respectively, whereas serogroup D further divided into two distinct genetic groups, D and E. Genetic group E was isolated in Okayama Prefecture, Japan, and all strains of it have been found to coexist with tumorigenic strains belonging to the other groups within the same galled grapevine tissues. These results suggest that A. vitis strains are genetically heterogeneous and can be separated into several genetic groups. The differences between the nucleotide sequences of pyrG, recA and rpoD of the genetic groups will enable the development of a simple and convenient monitoring method, which will increase understanding of the dynamics of each genetic group of A. vitis in the natural environment.

DOI： 10.1111/j.1365-3059.2008.01829.x

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Polyphosphate kinase is essential for swarming motility, tolerance to environmental stresses, and virulence in Pseudomonas syringae pv. tabaci 6605

Md. Mijan Hossain, Chiharu Tani, Tomoko Suzuki, Fumiko Taguchi, Tatsuhiro Ezawa, Yuki Ichinose

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   72 ( 4-6 )   122 - 127   2008年7月

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担当区分：最終著者,　責任著者   記述言語：英語   出版者・発行元：ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD  

Polyphosphate kinase (PPK), encoded by the ppk gene, is a principal enzyme responsible for synthesis of inorganic polyphosphate (poly P) from ATP in many Gram-negative bacteria. In order to elucidate the functions of poly P in Pseudomonas syringae pv. tabaci 6605, an in-frame deletion mutant of the ppk gene (Delta ppk) was constructed. The Delta ppk mutant did not accumulate poly P, whereas the wild-type strain accumulated a large quantity. The mutant had reduced swarming motility, even though it retains swimming motility like the parental strain. The mutant exhibited increased sensitivity to prolonged incubation and environmental stresses, such as heat shock and oxidative stress and reduced exopolysaccharide (EPS) production compared to the wild-type. Northern blot analysis revealed that expression of the rpoS gene, encoding the stationary phase sigma factor RpoS, was reduced in Delta ppk in the logarithmic phase, indicating that rpoS is regulated by the ppk gene. The poly P deficient mutant had significantly reduced ability to cause disease in its host tobacco plant and in planta growth of the mutant was also significantly reduced in host tobacco leaves as compared to the wild-type strain. Thus, our results suggest that poly P plays an important role in the virulence of P. syringae pv. tabaci 6605. (C) 2008 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.pmpp.2008.04.007

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Polyphosphate kinase is essential for swarming motility, tolerance to environmental stresses, and virulence in Pseudomonas syringae pv. tabaci 6605

Md. Mijan Hossain, Chiharu Tani, Tomoko Suzuki, Fumiko Taguchi, Tatsuhiro Ezawa, Yuki Ichinose

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   72 ( 4-6 )   122 - 127   2008年7月

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記述言語：英語   出版者・発行元：ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD  

Polyphosphate kinase (PPK), encoded by the ppk gene, is a principal enzyme responsible for synthesis of inorganic polyphosphate (poly P) from ATP in many Gram-negative bacteria. In order to elucidate the functions of poly P in Pseudomonas syringae pv. tabaci 6605, an in-frame deletion mutant of the ppk gene (Delta ppk) was constructed. The Delta ppk mutant did not accumulate poly P, whereas the wild-type strain accumulated a large quantity. The mutant had reduced swarming motility, even though it retains swimming motility like the parental strain. The mutant exhibited increased sensitivity to prolonged incubation and environmental stresses, such as heat shock and oxidative stress and reduced exopolysaccharide (EPS) production compared to the wild-type. Northern blot analysis revealed that expression of the rpoS gene, encoding the stationary phase sigma factor RpoS, was reduced in Delta ppk in the logarithmic phase, indicating that rpoS is regulated by the ppk gene. The poly P deficient mutant had significantly reduced ability to cause disease in its host tobacco plant and in planta growth of the mutant was also significantly reduced in host tobacco leaves as compared to the wild-type strain. Thus, our results suggest that poly P plays an important role in the virulence of P. syringae pv. tabaci 6605. (C) 2008 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.pmpp.2008.04.007

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Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction

Mizuri Marutani, Fumiko Taguchi, Yujiro Ogawa, Md. Mijan Hossain, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

MOLECULAR GENETICS AND GENOMICS   279 ( 4 )   313 - 322   2008年4月

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記述言語：英語   出版者・発行元：SPRINGER  

Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-Component system-defective mutants, Delta gacA and Delta gacS, and a double mutant, Delta gacA Delta gacS, were generated. These mutants produced smaller amounts of N-acyl homoserine lactones required for quorum sensing, had lost swarming motility, and had reduced expression of virulence-related hrp genes and the algT gene required for exopolysaccharide production. The ability of the mutants to cause disease symptoms in their host tobacco plant was remarkably reduced, while they retained the ability to induce hypersensitive reaction (HR) in the nonhost plants. These results indicated that the Gac two-component system of P. syringae pv. tabaci 6605 is indispensable for virulence on the host plant, but not for HR induction in the nonhost plants.

DOI： 10.1007/s00438-007-0309-y

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Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction

Mizuri Marutani, Fumiko Taguchi, Yujiro Ogawa, Md. Mijan Hossain, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

MOLECULAR GENETICS AND GENOMICS   279 ( 4 )   313 - 322   2008年4月

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担当区分：最終著者,　責任著者   記述言語：英語   出版者・発行元：SPRINGER  

Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-Component system-defective mutants, Delta gacA and Delta gacS, and a double mutant, Delta gacA Delta gacS, were generated. These mutants produced smaller amounts of N-acyl homoserine lactones required for quorum sensing, had lost swarming motility, and had reduced expression of virulence-related hrp genes and the algT gene required for exopolysaccharide production. The ability of the mutants to cause disease symptoms in their host tobacco plant was remarkably reduced, while they retained the ability to induce hypersensitive reaction (HR) in the nonhost plants. These results indicated that the Gac two-component system of P. syringae pv. tabaci 6605 is indispensable for virulence on the host plant, but not for HR induction in the nonhost plants.

DOI： 10.1007/s00438-007-0309-y

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その他リンク： http://link.springer.com/article/10.1007/s00438-007-0309-y/fulltext.html

Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana

Kuniaki Higashi, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

MOLECULAR GENETICS AND GENOMICS   279 ( 3 )   303 - 312   2008年3月

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記述言語：英語   出版者・発行元：SPRINGER  

Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.

DOI： 10.1007/s00438-007-0315-0

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Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana 国際誌

Kuniaki Higashi, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

MOLECULAR GENETICS AND GENOMICS   279 ( 3 )   303 - 312   2008年3月

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担当区分：最終著者,　責任著者   記述言語：英語   出版者・発行元：SPRINGER  

Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.

DOI： 10.1007/s00438-007-0315-0

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(72)タバコ野火病菌べん毛タンパク質フラジェリン糖鎖のべん毛構造・運動能における役割(関西部会講演要旨,平成19年度地域部会講演要旨)

田口 富美子, 柴田 敏史, 鈴木 智子, 小川 裕二郎, 相沢 慎一, 一瀬 勇規

日本植物病理學會報   74 ( 1 )   75 - 75   2008年2月

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記述言語：日本語   出版者・発行元：日本植物病理学会  

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Effects of glycosylation on swimming ability and flagellar polymorphic transformation in Pseudomonas syringae pv. tabaci 6605 国際誌

Fumiko Taguchi, Satoshi Shibata, Tomoko Suzuki, Yujiro Ogawa, Shin-Ichi Aizawa, Kasumi Takeuchi, Yuki Ichinose

JOURNAL OF BACTERIOLOGY   190 ( 2 )   764 - 768   2008年1月

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担当区分：最終著者,　責任著者   記述言語：英語   出版者・発行元：AMER SOC MICROBIOLOGY  

The role of flagellin glycosylation on motility was investigated in Pseudomonas syringae pv. tabaci. The swimming activity of glycosylation-defective mutants was prominently decreased in a highly viscous medium. The mutants showed differences in polymorphic transitions and in the bundle formation of flagella, indicating that glycosylation stabilizes the filament structure and lubricates the rotation of the bundle.

DOI： 10.1128/JB.01282-07

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Effects of glycosylation on swimming ability and flagellar polymorphic transformation in Pseudomonas syringae pv. tabaci 6605

Fumiko Taguchi, Satoshi Shibata, Tomoko Suzuki, Yujiro Ogawa, Shin-Ichi Aizawa, Kasumi Takeuchi, Yuki Ichinose

JOURNAL OF BACTERIOLOGY   190 ( 2 )   764 - 768   2008年1月

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記述言語：英語   出版者・発行元：AMER SOC MICROBIOLOGY  

The role of flagellin glycosylation on motility was investigated in Pseudomonas syringae pv. tabaci. The swimming activity of glycosylation-defective mutants was prominently decreased in a highly viscous medium. The mutants showed differences in polymorphic transitions and in the bundle formation of flagella, indicating that glycosylation stabilizes the filament structure and lubricates the rotation of the bundle.

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Molecular analysis for genome structure and gene expression of Oxidosqualene Cyclase genes in rice seedling

Yoshishige Inagaki, Yukako Mutsukado, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Anne Osbourn

GENES & GENETIC SYSTEMS   82 ( 6 )   521 - 521   2007年12月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：GENETICS SOC JAPAN  

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Flagellin Glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose

Kasumi Takeuchi, Hiroshi Ono, Mitsuru Yoshida, Tadashi Ishii, Etsuko Katoh, Fumiko Taguchi, Ryuji Miki, Katsuyoshi Murata, Hanae Kaku, Yuki Ichinose

JOURNAL OF BACTERIOLOGY   189 ( 19 )   6945 - 6956   2007年10月

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記述言語：英語   出版者・発行元：AMER SOC MICROBIOLOGY  

Flagellins from Pseudomonas syringae pv. glycinea race 4 and Pseudomonas syringae pv. tabaci 6605 have been found to be glycosylated. Glycosylation of flagellin is essential for bacterial virulence and is also involved in the determination of host specificity. Flagellin glycans from both pathovars were characterized, and common sites of glycosylation were identified on six serine residues (positions 143, 164, 176, 183, 193, and 201). The structure of the glycan at serine 201 (S201) of flagellin from each pathovar was determined by sugar composition analysis, mass spectrometry, and H-1 and C-13 nuclear magnetic resonance spectroscopy. These analyses showed that the S201 glycans from both pathovars were composed of a common unique trisaccharide consisting of two rhamnosyl (Rha) residues and one modified 4-amino-4,6-dideoxyglucosyl (Qui4N) residue, beta-D-Quip4N(3-hydroxy-1-oxobutyl)2Me-(1 -> 3)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap. Furthermore, mass analysis suggests that the glycans on each of the six serine residues are composed of similar trisaccharide units. Determination of the enantiomeric ratio of Rha from the flagellin proteins showed that flagellin from P. syringae pv. tabaci 6605 consisted solely Of L-Rha, whereas P. syringae pv. glycinea race 4 flagellin contained both L-Rha and D-Rha at a molar ratio of about 4:1. Taking these findings together with those from our previous study, we conclude that these flagellin glycan structures may be important for the virulence and host specificity of P. syringae.

DOI： 10.1128/JB.00500-07

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N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana

Kana Naito, Yasuhiro Ishiga, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Journal of General Plant Pathology   73 ( 4 )   281 - 285   2007年8月

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記述言語：英語  

Flagellin in Pseudomonas syringae is a potent elicitor of defense responses including hypersensitive cell death in dicot plants. The oligopeptides flg22 consisting of 22 conserved amino acids near the N-terminus of flagellins is reported to induce plant defense responses. Because glycosylation of the central domain of flagellin affects its elicitor activity, we investigated whether any peptide sequence in addition to flg22 is required for flagellin-induced hypersensitive reaction. A study of recombinant flagellin polypeptides indicated that the N-terminal domain including the conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana. © 2007 The Phytopathological Society of Japan and Springer.

DOI： 10.1007/s10327-007-0017-9

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Suppression of Cdc27B expression induces plant defence responses

Chikako Kudo, Tomoko Suzuki, Sumie Fukuoka, Shuta Asai, Hiroko Suenaga, Michiko Sasabe, Yoshitaka Takano, Tetsuro Okuno, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Yoshi-Shige Inagaki

MOLECULAR PLANT PATHOLOGY   8 ( 4 )   365 - 373   2007年7月

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記述言語：英語   出版者・発行元：BLACKWELL PUBLISHING  

Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY- 2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase- promoting complex or cyclosome ( APC/ C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus:: Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.

DOI： 10.1111/J.1364-3703.2007.00397.X

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Ultrastructural features of Mycosphaerella pinodes Infection and differenced in host susceptibility responses among Medicago truncatula Ecotypes.

Suzuki, T, Maeda, A, Hirose, M, Ichinose, Y, Toyoda, K, Shiraishi, T

J. Electr. Microsc. Technol. Med. Biol.   20 ( 2 )   167 - 168   2007年

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Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells

Michiko Sasabe, Kana Naito, Hiroko Suenaga, Takako Ikeda, Kazuhiro Toyodak, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

DNA SEQUENCE   18 ( 2 )   152 - 159   2007年

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記述言語：英語   出版者・発行元：INFORMA HEALTHCARE-TAYLOR & FRANCIS  

The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 ( NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow ( BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein ( GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.

DOI： 10.1080/10425170601060905

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Ultrastructural features of Mycosphaerella pinodes Infection and differenced in host susceptibility responses among Medicago truncatula Ecotypes.

Suzuki, T, Maeda, A, Hirose, M, Ichinose, Y, Toyoda, K, Shiraishi, T

J. Electr. Microsc. Technol. Med. Biol.   20 ( 2 )   167 - 168   2007年

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A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis 国際誌

Fumiko Taguchi, Yujiro Ogawa, Kasumi Takeuchi, Tomoko Suzuki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

JOURNAL OF BACTERIOLOGY   188 ( 24 )   8376 - 8384   2006年12月

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担当区分：最終著者,　責任著者   記述言語：英語   出版者・発行元：AMER SOC MICROBIOLOGY  

Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modification of flagellin. Although the function of Orf3 remained unclear, an orf3 deletion mutant (Delta orf3 mutant) had reduced virulence on tobacco plants. Orf3 shows significant homology to a 3-oxoacyl-(acyl carrier protein) synthase III in the fatty acid elongation cycle. The Delta orf3 mutant had a significantly reduced ability to form acyl homoserine lactones (AHLs), which are quorum-sensing molecules, suggesting that Orf3 is required for AHL synthesis. In comparison with the wild-type strain, swarming motility, biosurfactant production, and tolerance to H2O2 and antibiotics were enhanced in the Delta orf3 mutant. A scanning electron micrograph of inoculated bacteria on the tobacco leaf surface revealed that there is little extracellular polymeric substance matrix surrounding the cells in the Delta orf3 mutant. The phenotypes of the Delta orf3 mutant and an AHL synthesis (Delta psyI) mutant were similar, although the mutant-specific characteristics were more extreme in the Delta orf3 mutant. The swarming motility of the Delta orf3 mutant was greater than that of the Delta psyI mutant. This was attributed to the synergistic effects of the overproduction of biosurfactants and/or alternative fatty acid metabolism in the Delta orf3 mutant. Furthermore, the amounts of iron and biosurfactant seem to be involved in biofilm development under quorum-sensing regulation in P. syringae pv. tabaci 6605.

DOI： 10.1128/JB.00763-06

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A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis

Fumiko Taguchi, Yujiro Ogawa, Kasumi Takeuchi, Tomoko Suzuki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

JOURNAL OF BACTERIOLOGY   188 ( 24 )   8376 - 8384   2006年12月

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記述言語：英語   出版者・発行元：AMER SOC MICROBIOLOGY  

Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modification of flagellin. Although the function of Orf3 remained unclear, an orf3 deletion mutant (Delta orf3 mutant) had reduced virulence on tobacco plants. Orf3 shows significant homology to a 3-oxoacyl-(acyl carrier protein) synthase III in the fatty acid elongation cycle. The Delta orf3 mutant had a significantly reduced ability to form acyl homoserine lactones (AHLs), which are quorum-sensing molecules, suggesting that Orf3 is required for AHL synthesis. In comparison with the wild-type strain, swarming motility, biosurfactant production, and tolerance to H2O2 and antibiotics were enhanced in the Delta orf3 mutant. A scanning electron micrograph of inoculated bacteria on the tobacco leaf surface revealed that there is little extracellular polymeric substance matrix surrounding the cells in the Delta orf3 mutant. The phenotypes of the Delta orf3 mutant and an AHL synthesis (Delta psyI) mutant were similar, although the mutant-specific characteristics were more extreme in the Delta orf3 mutant. The swarming motility of the Delta orf3 mutant was greater than that of the Delta psyI mutant. This was attributed to the synergistic effects of the overproduction of biosurfactants and/or alternative fatty acid metabolism in the Delta orf3 mutant. Furthermore, the amounts of iron and biosurfactant seem to be involved in biofilm development under quorum-sensing regulation in P. syringae pv. tabaci 6605.

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ダイズ斑点細菌病菌のフラジェリン糖鎖解析

竹内香純, 田口富美子, 加藤悦子, 三木隆二, 村田勝義, 賀来華江, 一瀬勇規

日本植物病理学会報   72 ( 4 )   310   2006年11月

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記述言語：日本語  

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Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen

Tomonari Kasai, Tomoko Suzuki, Kozue Ono, Ken'Ichi Ogawa, Yoshishige Inagaki, Yuki Ichinose, Kazuhiro Toyoda, Tomonori Shiraishi

Journal of General Plant Pathology   72 ( 5 )   265 - 272   2006年10月

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記述言語：英語  

We previously reported that the release of O2 - from isolated pea cell walls was enhanced by a 70-kDa glycoprotein elicitor but was suppressed by mucin-type glycopeptide suppressors (supprescins A and B) prepared from pycnospore germination fluid of Mycosphaerella pinodes, causal agent of Mycosphaerella blight of pea. Here, we show that superoxide dismutase (SOD) in the apoplast fluid/cell wall of pea seedlings responds to the fungal elicitor and suppressor molecules. In a pharmacological study and with internal amino acid sequencing, the apoplastic SOD in a pea cultivar Midoriusui was found to be a Cu/Zn type SOD. We cloned a full-length cDNA of the Cu/Zn-SOD and designated it as PsCu/Zn-SOD1. An increase in PsCu/Zn-SOD1 mRNA and the PsCu/Zn-SOD1 protein was induced by treatment with the elicitor more intensively than by wounding. Such induction by the elicitor or wounding, however, was inhibited by the concomitant presence of supprescins. The SOD activity of recombinant PsCu/Zn-SOD1 was regulated directly by these signal molecules in a manner similar to their effect on the SOD activity in the apoplastic fluid and in the cell wall-bound proteins. Based on these findings, we discuss a role for PsCu/Zn-SOD1 in the pea defense response. © 2006 The Phytopathological Society of Japan and Springer-Verlag.

DOI： 10.1007/s10327-006-0283-y

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A binding protein for fungal signal molecules in the cell wall of Pisum sativum

Akinori Kiba, Takako Ohgawara, Kazuhiro Toyoda, Miho Inoue-Ozaki, Tadahiro Takeda, Uppalapati Srinivasa Rao, Toshiaki Kato, Yuki Ichinose, Tomonori Shiraishi

Journal of General Plant Pathology   72 ( 4 )   228 - 237   2006年8月

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記述言語：英語  

In the plant cell wall of Pisum sativum seedlings, we found an NTPase (E.C. 3.6.1.5.) with ATP-hydrolyzing activity that was regulated by an elicitor and suppressors of defense from pea pathogen Mycosphaerella pinodes. The ATPase-rich fraction was purified from pea cell walls by NaCl solubilization, ammonium sulfate precipitation, and chromatography with an ATP-conjugated agarose column and an anion-exchange column. The specific activity of the final ATPase-rich fraction increased 600-fold over that of the initial NaCl-solubilized fraction. The purified ATPase-rich fraction also had peroxidase activity and generated superoxide, both of which were regulated by the M. pinodes elicitor and suppressor (supprescins). Active staining and Western blot analysis also showed that the ATPase was copurified along with peroxidases. In this fraction, a biotinylated elicitor and the supprescins were bound primarily and specifically to ca. 55-kDa protein (CWP-55) with an N-terminal amino acid sequence of QEEISSYAVVFDA. The cDNA clone of CWP-55 contained five ACR domains, which are conserved in the apyrases (NTPases), and the protein is identical to a pea NTPase cDNA (GenBank accession AB071369). Based on these results, we discuss a role for the plant cell wall in recognizing exogenous signal molecules. © The Phytopathological Society of Japan and Springer-Verlag 2006.

DOI： 10.1007/s10327-006-0278-8

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A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms

Akinori Kiba, Kazuhiro Toyoda, Kazuaki Yoshioka, Kami Tsujimura, Hirotaka Takahashi, Yuki Ichinose, Tadahiro Takeda, Toshiaki Kato, Tomonori Shiraishi

Journal of General Plant Pathology   72 ( 4 )   238 - 246   2006年8月

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記述言語：英語  

Apyrases (E.C.3.6.1.5
NTP-NDPases) are distributed in the cytosol, nuclei, cytoskeleton, and on the surface of plant cells. Some may play an important role in signal transduction from exogenous stimuli. We previously found a protein of ca. 55-kDa (CWP-55) in an ATPase-rich fraction from the pea cell wall bound to the elicitor and supprescins (suppressors of defense) from pea pathogen Mycosphaerella pinodes. We cloned the cDNA of CWP-55 that coincided with PsAPY1, one of two NTPase clones in a pea cDNA library. An analysis with a green fluorescent protein fusion protein indicated that PsAPY1 was distributed in the cell wall, nucleus, and cytoplasm. The recombinant PsAPY1 expressed in Escherichia coli had ATP-hydrolyzing activity responsive not only to the elicitor and supprescins from the pea pathogen but also to other elicitors such as a bacterial harpin, a yeast extract, and a synthetic glycopeptide. Biotinylated fungal signal molecules were bound to the recombinant PsAPY1 specifically. Resonant mirror detection confirmed such binding characteristics of PsAPY1. Based on these results, we discuss the role of cell-wall-bound NTPases in recognizing and responding to microorganisms on the cell wall surface. © The Phytopathological Society of Japan and Springer-Verlag 2006.

DOI： 10.1007/s10327-006-0279-7

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Localization and responsiveness of a cowpea apyrase VsNTPase1 to phytopathogenic microorganisms

Hirotaka Takahashi, Kazuhiro Toyoda, Yuzo Hirakawa, Kunihiko Morishita, Toshiaki Kato, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Journal of General Plant Pathology   72 ( 3 )   143 - 151   2006年6月

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記述言語：英語  

Apyrases (NTPases) are associated with both compatible and incompatible interactions between plants and microorganisms. Previously we reported that the ATPase activities of cell-wall-bound apyrases of several leguminous plants, such as pea, cowpea, soybean, and kidney bean, were enhanced by a glycoprotein elicitor and were inhibited in a species-specific manner by mucin-type glycopeptide suppressors secreted from a pea pathogenic fungus, Mycosphaerella pinodes. In this study, we isolated two apyrase genes, VsNTPase1 and VsNTPase2, from a cDNA library of Vigna sinensis Endl. cv. Sanjakusasage. Based on phylogenetic analysis, VsNTPase1 may belong to a group that responds to environmental stimuli. In a transient assay using DNA bombardment, a fusion protein of green fluorescent protein (GFP) and the N-terminal putative signal sequence of VsNTPase1 was distributed in the nucleus, cytoplasm (cytoskeletal structure), and cell wall. On the other hand, a fusion protein of GFP and the N-terminal putative VsNTPase2-signal sequence was localized in the cytoplasm, especially in small particles (perhaps mitochondria). A recombinant VsNTPase1 expressed in Spodoptera frugiperda 21 cells responded directly to signal molecules from several phytopathogenic microorganisms. Here, we discuss the role of apyrases in recognizing and responding to exogenous signals. © The Phytopathological Society of Japan and Springer-Verlag 2006.

DOI： 10.1007/s10327-005-0267-3

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Identification of glycosylation genes and glycosylated amino acids of flagellin in Pseudomonas syringae pv. tabaci

F Taguchi, K Takeuchi, E Katoh, K Murata, T Suzuki, M Marutani, T Kawasaki, M Eguchi, S Katoh, H Kaku, C Yasuda, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

CELLULAR MICROBIOLOGY   8 ( 6 )   923 - 938   2006年6月

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記述言語：英語   出版者・発行元：BLACKWELL PUBLISHING  

A glycosylation island is a genetic region required for glycosylation. The glycosylation island of flagellin in Pseudomonas syringae pv. tabaci 6605 consists of three orfs: orf1, orf2 and orf3. Orf1 and orf2 encode putative glycosyltransferases, and their deletion mutants, Delta orf1 and Delta orf2, exhibit deficient flagellin glycosylation or produce partially glycosylated flagellin respectively. Digestion of glycosylated flagellin from wild-type bacteria and non-glycosylated flagellin from Delta orf1 mutant using aspartic N-peptidase and subsequent HPLC analysis revealed candidate glycosylated amino acids. By generation of site-directed Ser/Ala-substituted mutants, all glycosylated amino acid residues were identified at positions 143, 164, 176, 183, 193 and 201. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) analysis revealed that each glycan was about 540 Da. While all glycosylation-defective mutants retained swimming ability, swarming ability was reduced in the Delta orf1, Delta orf2 and Ser/Ala-substituted mutants. All glycosylation mutants were also found to be impaired in the ability to adhere to a polystyrene surface and in the ability to cause disease in tobacco. Based on the predicted tertiary structure of flagellin, S176 and S183 are expected to be located on most external surface of the flagellum. Thus the effect of Ala-substitution of these serines is stronger than that of other serines. These results suggest that glycosylation of flagellin in P. syringae pv. tabaci 6605 is required for bacterial virulence. It is also possible that glycosylation of flagellin may mask elicitor function of flagellin molecule.

DOI： 10.1111/j.1462-5822.2005.0674.x

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Induction of defense responses in pea tissues by inorganic phosphate

Tomoharu Kawahara, Hiroko Namba, Kazuhiro Toyoda, Tomonari Kasai, Megumi Sugimoto, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Journal of General Plant Pathology   72 ( 3 )   129 - 136   2006年6月

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記述言語：英語  

When inorganic phosphate, a common and essential element for organisms, was applied endogenously, a rejection reaction and superoxide generation were induced in pea tissues but phytoalexin production was not. Phosphate-induced superoxide generation was sensitive to cycloheximide (CHX) and salicylhydroxamic acid (SHAM), indicating that part of the generation was dependent upon the expression of peroxidase gene(s). Peroxidases (POXs) are well known not only to scavenge hydrogen peroxide with phenolics but also to generate superoxide via NADH oxidation in the presence of p-coumaric acid and manganese ion. We cloned five pea POX cDNAs that are predicted to be located outside of the cells. The accumulation of five POX mRNAs, NTPase mRNA, and phenylalanine ammonia-lyase mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction. The expression of the five POX genes was induced by a fungal elicitor. On the other hand, inorganic phosphate induced the accumulation of POX11, POX14, and POX21 mRNAs but not of POX13, POX29, and PsPAL1 mRNAs within 1-3∈h after treatment of pea seedlings. In view of these findings, we discuss inorganic phosphate as a signal transmitter inducing part of the plant defense responses. © The Phytopathological Society of Japan and Springer-Verlag 2006.

DOI： 10.1007/s10327-005-0261-9

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Functional analysis of Gac two-component system in P syringae pv. tabaci

M. Marutani, Y. Ogawa, F. Taguchi, Y. Inagaki, K. Toyoda, T. Shiraishi, Y. Ichinose

PHYTOPATHOLOGY   96 ( 6 )   S74 - S74   2006年6月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER PHYTOPATHOLOGICAL SOC  

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植物の非宿主抵抗性

一瀬勇規

化学と生物   44 ( 8 )   556 - 562   2006年

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記述言語：日本語   出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/kagakutoseibutsu1962.44.556

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その他リンク： https://jlc.jst.go.jp/DN/JALC/00283065642?from=CiNii

AtOPR3高発現シロイヌナズナを用いた植物病原菌相互作用におけるOPR subgroup 2の機能解析

松井英譲, 豊田和弘, 稲垣善茂, 白石友紀, 一瀬勇規

日本植物病理学会報   72 ( 4 )   2006年

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(18) 病原性相互作用で発現が変動する植物遺伝子の機能解析2 : 小胞体-ゴルジ間の小胞輸送に関わるRer1遺伝子ホモログの病原菌応答(平成17年度日本植物病理学会大会講演要旨)

吉廣 美由貴, 廣瀬 昌也, 藤田 景子, 稲垣 善茂, 一瀬 勇規, 豊田 和弘, 白石 友紀

日本植物病理學會報   71 ( 3 )   190 - 190   2005年8月

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記述言語：日本語   出版者・発行元：日本植物病理学会  

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Flagellin from Pseudomonas syringae pv. tabaci induced hrp-independent HR in tomato

Mizuri Marutani, Fumiko Taguchi, Rena Shimizu, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Journal of General Plant Pathology   71 ( 4 )   289 - 295   2005年8月

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記述言語：英語  

We have previously shown that flagellin of Pseudomonas syringae pv. tabaci is an elicitor that induces a hypersensitive reaction (HR) in nonhost tomato cells. Flagellin is the major HR elicitor produced by this pathogen, as shown by the inability of a flagellin-defective mutant, ΔfliC, to induce HR. Also, a ΔfliD mutant that secretes large amounts of monomer flagellins induces a strong HR in tomato. In this study, the possible involvement of an Hrp type III secretion system (TTSS) in flagellin-induced HR was investigated using flagella-defective mutants or Hrp TTSS-defective mutants. The hrcC gene encodes HrcC protein, which is required for Hrp pilus formation in the outer membrane. An hrcC mutation, introduced into the wild-type, ΔfliC, and ΔfliD mutants of P. syringae pv. tabaci did not affect swimming motility or flagellin secretion, whereas all ΔhrcC, ΔfliC, and ΔfliD mutants lost the ability to cause disease on host tobacco leaves. However, the ΔhrcC mutant and the ΔfliD/ΔhrcC double mutant were still able to induce HR cell death, expression of one of the defense-related genes hsr203J, and the generation of hydrogen peroxide in nonhost tomato cells. Thus, flagellin is required for both pathogenicity in host tobacco and HR in nonhost tomato. On the other hand, hrp TTSS is necessary for pathogenicity on host tobacco but is not indispensable to induce HR in nonhost tomato. These results clearly show that flagellin-induced HR is hrp-independent in tomato. © The Phytopathological Society of Japan and Springer-Verlag 2005.

DOI： 10.1007/s10327-005-0200-9

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Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)

Yasuhiro Ishiga, Kasumi Takeuchi, Fumiko Taguchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Journal of General Plant Pathology   71 ( 4 )   302 - 307   2005年8月

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記述言語：英語  

Flagellin, an essential component of the bacterial flagellar filament, is capable of inducing a hypersensitive response (HR), including cell death, in a nonhost plant. A flagellin-defective mutant (ΔfliC) of Pseudomonas syringae pv. tabaci lacks both the flagellar filament and motility, whereas a flagellin-glycosylation-defective mutant (Δorf1) retains the flagellar filament but lacks the glycosyl modification of flagellin protein. To investigate the role of flagellin protein and its glycosylation in the interaction with its nonhost Arabidopsis thaliana, we analyzed plant responses after inoculation with these bacteria. Inoculation with wild-type P. syringae pv. tabaci induced HR, with the generation of reactive oxygen species and cell death. In contrast, inoculation with either ΔfliC or Δorf1 mutant induced a low level of HR, and inoculated leaves developed a disease-like yellowing. These mutant bacteria multiplied better than the wild-type bacteria in A. thaliana. These results indicate that A. thaliana expresses a defense reaction in response to the bacterial flagellin with its glycosyl structure. © The Phytopathological Society of Japan and Springer-Verlag 2005.

DOI： 10.1007/s10327-005-0201-8

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Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (∆fliC) and flagellin-glycosylation (∆orf1).

J. Gen. Plant Pathol.   71 ( 4 )   302 - 307   2005年8月

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記述言語：英語  

DOI： 10.1007/s10327-005-0201-8

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Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells

Dirk Schenke, Kana Naito, Kazuhiro Toyoda, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

Journal of General Plant Pathology   71 ( 4 )   273 - 279   2005年8月

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記述言語：英語  

INF1 elicitin, a proteinaceous elicitor produced by Phytophthora infestans, induces a hypersensitive response in tobacco BY-2 cells. In response to elicitin, tobacco cells produce both reactive oxygen species (ROS) and ethylene (ET). To investigate the regulation of elicitin-induced ET production, we pharmacologically analyzed the effects of several chemicals on ET production. Inhibitors of ROS generation or ROS chelators efficiently inhibited ET production, whereas simultaneous treatment of a superoxide anion-generating system with salicylhydroxamic acid recovered ET production. In an in vitro experiment, superoxide anion was necessary and sufficient for conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ET because ET was produced from ACC solely in the presence of the superoxide-generating chemical KO2. ET production was also inhibited by lipoxygenase (LOX) inhibitors, indicating a possible involvement of LOX-mediated generation of superoxide anion and ET production itself. Furthermore, elicitin-induced ET production was completely inhibited by the protein synthesis inhibitor cycloheximide but recovered after exogenous application of ACC, indicating that de novo protein synthesis is required for ACC accumulation, leading to ET production. We also investigated the effects of several phytohormones on elicitor-induced ET production and discuss their role in the defense response. © The Phytopathological Society of Japan and Springer-Verlag 2005.

DOI： 10.1007/s10327-005-0197-0

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Identification of genes expressed during spore germination of Mycosphaerella pinodes

Hiroyuki Takahara, Kazuhiro Toyoda, Gento Tsuji, Yasuyuki Kubo, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Journal of General Plant Pathology   71 ( 3 )   190 - 195   2005年6月

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記述言語：英語  

Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection. © The Phytopathological Society of Japan and Springer-Verlag 2005.

DOI： 10.1007/s10327-005-0185-4

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植物病原細菌Pseudomonas syringaeのべん毛タンパク質フラジェリンの糖鎖修飾と植物相互作用

竹内香純, 田口富美子, 三木隆二, 安田千裕, 村田勝義, 加藤悦子, 加藤静恵, 賀来華江, 稲垣善茂, 豊田和弘, 白石友紀, 一瀬勇規

植物微生物研究会研究交流会講演要旨集   14th   (JA)120,(EN)121   2005年3月

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記述言語：日本語  

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Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens

Hideki Takahashi, Ayano Shimizu, Tsutomu Arie, Syofi Rosmalawati, Sumire Fukushima, Mari Kikuchi, Yasufumi Hikichi, Ayami Kanda, Akiko Takahashi, Akinori Kiba, Kohei Ohnishi, Yuki Ichinose, Fumiko Taguchi, Chihiro Yasuda, Motoichiro Kodama, Mayumi Egusa, Chikara Masuta, Hiroyuki Sawada, Daisuke Shibata, Koichi Hori, Yuichiro Watanabe

Journal of General Plant Pathology   71 ( 1 )   8 - 22   2005年2月

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記述言語：英語  

Lycopersicon esculentum cultivar Micro-Tom is a miniature tomato with many advantages for studies of the molecular biology and physiology of plants. To evaluate the suitability of Micro-Tom as a host plant for the study of pathogenesis, Micro-Tom plants were inoculated with 16 well-known fungal, bacterial, and viral pathogens of tomato. Athelia rolfsii, Botryotinia fuckeliana, Oidium sp., Phytophthora infestans, and Sclerotinia sclerotiorum caused typical symptoms and sporulated abundantly on Micro-Tom. Micro-Tom was resistant to Alternaria alternata, Corynespora cassiicola, and Fusarium oxysporum. When Micro-Tom was inoculated with 17 isolates of Ralstonia solanacearum, many isolates induced wilt symptoms. Agrobacterium tumefaciens also was pathogenic, causing crown galls on stem tissue after needle prick inoculation. In Micro-Tom sprayed with Pseudomonas syringae pv. tomato, P. s. pv. tabaci, or P. s. pv. glycinea, bacterial populations did not increase, and yellow lesions appeared only on leaves sprayed with P. s. pv. tomato. Tomato mosaic virus, Tomato aspermy virus, and Cucumber mosaic virus systemically infected Micro-Tom, which developed symptoms characteristic of other cultivars of tomato after infection with the respective virus. These results indicated that Micro-Tom was generally susceptible to most of the important tomato pathogens and developed typical symptoms, whereas certain pathogens were restricted by either hypersensitive resistance or nonhost resistance on Micro-Tom. Therefore, an assortment of Micro-Tom-pathogen systems should provide excellent models for studying the mechanism of susceptible and resistant interactions between plants and pathogens. © The Phytopathological Society of Japan and Springer-Verlag 2005.

DOI： 10.1007/s10327-004-0168-x

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Identification of the genes expressed during spore germination of Mycosphaerella pinodes.

J. Gen. Plant Pathol.   71 (3), 190-195.   2005年

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DOI： 10.1007/s10327-005-0185-4

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Pea apoplastic Cu/Zn-SOD responsive to pathogenic signal molecules

T Kasai, K Ono, T Suzuki, K Toyoda, Y Inagaki, Y Ichinose, T Shiraishi

PLANT AND CELL PHYSIOLOGY   46   S94 - S94   2005年

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：OXFORD UNIV PRESS  

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Mechanism of flagellin-induced hypersensitive cell death in Arabidopsis thaliana

Y Ishiga, YC Lin, M Marutani, F Taguchi, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

PLANT AND CELL PHYSIOLOGY   46   S94 - S94   2005年

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：OXFORD UNIV PRESS  

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Pseudomonas syringaeのべん毛を介した植物相互作用

一瀬勇規, 田口富美子, 竹内香純, 石賀康博, 村田勝義, 加藤悦子, 丸谷瑞理, 三木隆二, 安田千裕

日本植物病理学会植物感染生理談話会論文集   ( 40 )   13 - 24   2004年8月

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記述言語：日本語  

J-GLOBAL

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Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis of Colletotrichum trifolii

Hiroyuki Takahara, Gento Tsuji, Yasuyuki Kubo, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Journal of General Plant Pathology   70 ( 2 )   93 - 96   2004年4月

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記述言語：英語  

We transformed Colletotrichum trifolii, the causal agent of alfalfa anthracnose, using Agrobacterium tumefaciens as a new tool for random insertional mutagenesis. Fungal spores of C. trifolii were transformed with T-DNA including the hygromycin phosphotransferase gene (hph). Southern analysis showed that every randomly selected transformant had a unique hybridization pattern of T-DNA, suggesting that the T-DNA was randomly integrated into the fungal genome. More significantly, about 75% of transformants had a single copy of the T-DNA. The results demonstrate that insertional mutagenesis via A. tumefaciens is a useful tool for studying the function of C. trifolii genes.

DOI： 10.1007/s10327-003-0099-y

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Structure and expression of 12-oxophytodienoate reductase (subgroup I) genes in pea, and characterization of the oxidoreductase activities of their recombinant products

H Matsui, G Nakamura, Y Ishiga, H Toshima, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

MOLECULAR GENETICS AND GENOMICS   271 ( 1 )   1 - 10   2004年2月

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担当区分：最終著者,　責任著者   記述言語：英語   出版者・発行元：SPRINGER-VERLAG  

Recently, we observed that expression of a pea gene (S64) encoding an oxophytodienoic acid reductase (OPR) was induced by a suppressor of pea defense responses, secreted by the pea pathogen Mycosphaerella pinodes. Because it is known that OPRs are usually encoded by families of homologous genes, we screened for genomic and cDNA clones encoding members of this putative OPR family in pea. We isolated five members of the OPR gene family from a pea genomic DNA library, and amplified six cDNA clones, including S64, by RT-PCR (reverse transcriptase-PCR). Sequencing analysis revealed that S64 corresponds to PsOPR2, and the amino acid sequences of the predicted products of the six OPR-like genes shared more than 80% identity with each other. Based on their sequence similarity, all these OPR-like genes code for OPRs of subgroup I, i.e., enzymes which are not required for jasmonic acid biosynthesis. However, the genes varied in their exon/intron organization and in their promoter sequences. To investigate the expression of each individual OPR-like gene, RT-PCR was performed using gene-specific primers. The results indicated that the OPR-like gene most strongly induced by the inoculation of pea plants with a compatible pathogen and by treatment with the suppressor from M. pinodes was PsOPR2. Furthermore, the ability of the six recombinant OPR-like proteins to reduce a model substrate, 2-cyclohexen-1-one (2-CyHE), was investigated. The results indicated that PsOPR1, 4 and 6 display robust activity, and PsOPR2 has a most remarkable ability to reduce 2-CyHE, whereas PsOPR3 has little and PsOPR5 does not reduce this compound. Thus, the six OPR-like proteins can be classified into four types. Interestingly, the gene structures, expression profiles, and enzymatic activities used to classify each member of the pea OPR-like gene family are clearly correlated, indicating that each member of this OPR-like family has a distinct function.

DOI： 10.1007/s00438-003-0948-6

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Differential regulation of MBP kinases by a glycoproptein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea

Uppalapati, SR, K Toyoda, Yasuhiro, I, Y Ichinose, T Shiraishi

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   64 ( 1 )   17 - 25   2004年1月

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記述言語：英語   出版者・発行元：ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

A polypeptide fungal suppressor from a pea pathogen Mycosphaerella pinodes plays a key role in pathogenesis by suppressing elicitor-induced defense response(s) in pea (Pisum sativum L). In this study, we show that treatment of pea tissues with the polysaccharide elicitor secreted by M. pinodes results in rapid increased activation of two myelin basic protein (MBP)-dependent kinases p44 (approximate to44 kDa) and p48 (approximate to48 kDa) within 15-30 min upon elicitation. Interestingly, the suppressor inhibited the elicitor-induced activation of only p44 kinase. While the defense-inducing signalling molecules, chitosan and salicylic acid (SA) activated the p44 and p48 kinases, methyl jasmonate (MeJA) did not. The abiotic stress signals, abscisic acid (ABA), NaCl and wounding activated the p48 kinase alone. These results demonstrate that MAPKs are differentially activated in response to pathogen invasion and abiotic stress in pea. Furthermore, specific inhibition of elicitor-induced p44 kinase activation by a MAPKK inhibitor, PD098059 and protein kinase inhibitor, K252a correlated with the suppression of elicitor-induced phenylalanine ammonia lyase (PAL) gene expression, supporting a role for p44 in the elicitor-induced defense response(s) in pea. Inhibition of p44 by the phosphoinositide (PI) turnover inhibitor, neomycin (a fungal suppressor mimic), and potentiation of p44 by the diacylglycerol (DAG) kinase inhibitor, R59022 indicated that p44 may be acting downstream of (PI) metabolism. Taken together, our results indicate that suppressor of defense elicitation from M. pinodes acts through inhibition of a MAPK (p44), possibly through a PI signaling pathway, facilitating the establishment of basic compatibility during infection of pea. (C) 2004 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.pmpp.2004.05.003

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耐病性のシグナリングモデル，新

分子レベルからみた植物の耐病性 (細胞工学別冊)，(島本 功ら編) 秀潤社 (東京)   pp.14-17.   2004年

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植物の病原菌に体する受容性と抵抗性

新版 分子レベルからみた植物の耐病性 (細胞工学別冊)，(島本 功ら編) 秀潤社   pp.74-81.   2004年

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PDR-type ABC transporter and tobacco defense response against pathogen

Y Ichinose, D Schenke, M Sasabe, Y Inagaki, K Toyoda, T Shiraishi

PLANT AND CELL PHYSIOLOGY   45   S1 - S1   2004年

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：OXFORD UNIV PRESS  

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Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101.

Genes Genet. Syst.   78 ( 6 )   409 - 418   2003年12月

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担当区分：最終著者,　責任著者   記述言語：英語  

DOI： 10.1266/ggs.78.409

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Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response

F Taguchi, R Shimizu, R Nakajima, K Toyoda, T Shiraishi, Y Ichinose

PLANT PHYSIOLOGY AND BIOCHEMISTRY   41 ( 2 )   165 - 174   2003年2月

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記述言語：英語   出版者・発行元：EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER  

To investigate the factor that determines incompatible interactions between Pseudomonas syringae pv. tabaci and non-host plants, an elicitor of hypersensitive reaction (HR) was partially purified from the supernatant of a nutrient-poor medium of bacterial culture by DEAE column chromatography. The major protein in the elicitor-active fractions was identified as a flagellin which is a component of flagellar filaments. The flagellins purified from P. syringae pv. tomato and glycinea, incompatible pathogens of tobacco plants, induced fragmentation of chromosomal DNA and oxidative burst accompanied by programmed cell death in tobacco (Nicotiana tabacum) Bright Yellow (BY-2) cells, but the flagellin from pv. tabaci, a compatible pathogen, did not. However, the amino acid sequences of flagellins deduced from fliC genes showed a high homology among these P. syringae pathovars. In particular, the amino acid sequences of pv. tabaci and glycinea are completely identical. However, both recombinant flagellins produced in Escherichia coli possess HR-inducing activity in BY-2 cells. These results indicate that the post-translational modification of flagellins has an important role for HR-inducing ability in tobacco cells. Furthermore, we discuss the cause of a different elicitor activity among flagellins on tobacco cells and the role of flagellins in the determining specificity. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

DOI： 10.1016/S0981-9428(02)00018-9

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Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea.

The Scientific Reports of The Faculty of Agriculture, Okayama University.   92, in press   2003年

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Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants, DfliC and DfliD, in host tobacco plants.

J. Gen. Plant Pathol.   69 (4) 244-249.   2003年

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DOI： 10.1007/s10327-003-0045-z

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Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants, DfliC and DfliD, in host tobacco plants.

J. Gen. Plant Pathol.   69 (4) 244-249.   2003年

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DOI： 10.1007/s10327-003-0045-z

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Role of flagella and flagellin in plant-Pseudomonas syrignae interactions. In Japan/ Taiwan Symposium on Molecular Biology of Functional Regulation in Plant and Microbe (N. Sako, H. Yaegashi and M.-K. Yang eds.)

Showado Co. (Saga, Japan)   pp. 158-166.   2003年

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Phylogenetic Classification of Dof type Transcription Factors in Pea (Pisum sativum)

Naoki Nakamura, Mizuri Marutani, Shiroh Sanematsu, Kazuhiro Toyoda, Yoshi-shige Inagaki, Tomonori Shiraishi, Yuki Ichinose

Plant Biotechnology   20 ( 3 )   247 - 252   2003年

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記述言語：英語  

The Dof protein family is a group of plant transcription factors carrying highly conserved 52 residues referred to as the Dof domain, and belongs to the C4 zinc-finger transcription factors. We isolated various PsDof genes by PCR using the Dof-domain nucleotide sequence of the PsDofl gene from a cDNA library of elicitor-treated pea epicotyls, and these isolated PsDof genes were then classified phylogenetically. Since the obtained genes (PsDofl to PsDofT) were scattered over various positions of the phylogenetic tree, they were expected to perform various functions as Dof-type transcription factors. From their positions in the tree, it is expected that the PsDofZ and PsDof5 genes are defense related, as is the PsDofl gene. © 2003, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.

DOI： 10.5511/plantbiotechnology.20.247

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Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens.

J. Gen. Plant Pathol.   69 (6) In press   2003年

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DOI： 10.1007/s10327-003-0061-z

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Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens.

J. Gen. Plant Pathol.   69 (6) In press   2003年

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DOI： 10.1007/s10327-003-0061-z

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Role of Flagella and Flagellin in Plant - Pseudomonas syringae Interactions

Pseudomonas syringae and Related Pathogens (N. S. Iacobellis ed.) Kluwer AcademicPress (Dordrecht, The Netherland)   pp. 311-318.   311 - 318   2003年

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担当区分：筆頭著者,　責任著者   記述言語：英語  

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Post-translational modification of flagellin determines the specificity of HR induction.

Plant Cell Physiol.   44 (3) 342-349.   2003年

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DOI： 10.1093/pcp/pcg042

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植物病原細菌のべん毛を介した植物相互作用．

一瀬 勇規, 田口 富美子, 稲垣 善茂, 豊田 和弘, 白石 友紀

化学と生物   41 (8) 511-516 ( 8 )   511 - 516   2003年

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記述言語：日本語   出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/kagakutoseibutsu1962.41.511

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Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea.

Seki Hikaru, Marutani Mizuri, Inagaki Yoshishige, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

The Scientific Reports of The Faculty of Agriculture, Okayama University.   92, in press ( 1 )   21 - 26   2003年

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記述言語：英語   出版者・発行元：岡山大学農学部  

Recently, we, isolated cDNA clone, PsDof1, from clicitor-treated pea cDNA library. The putative gene product, a PsDof1, encodes DNA binding protein that specifically binds the DNA fragment containing AAAG core sequence. In this paper we report that GST-PsDof1 fusion protein specifically binds to the promoter region containing AAAG core sequence(s) of PsCHS1, one of the elicitor-inducible genes encoding chalcone synthase (CHS). Furthermore the addition of DNA fragment containing AAAG motif to the 35S minimal promoter provided the elicitor-responsibility in transient transfection assay using pea protoplasts. These results suggest that PsDof1 might be involved in defense responses by acitivating the transcription by a binding to AAAG core sequence in the promoter of the defense-related genes in pea.エリシターを処理したエンドウ上胚軸由来のRNAから作成されたcDNAライブラリーからエリシター処理により発現が増高する遺伝子候補のcDNAとしてPsDof1が単離された．大腸菌で生産されたGST-PsDof１融合タンパク質はAAAG配列をコアとするDNAに結合することが明らかにされている．本論文ではGST-PsDof１がエリシター応答性遺伝子の一つ，PsCHS1のプロモーター上のAAAGまたはCTTT配列を有する断片に特異的に結合することを明らかにした．更にAAAG配列のエリシター応答性シスエレメントとしての機能を解析するため，AAAG配列を４回繰り返したユニットをCaMV35Ｓの最小プロモーターとCATレポーター遺伝子に連結したキメラ遺伝子を構築し，エンドウプロトプラストにエレクトロポレーション法により導入した．CAT活性を指にプロモーター活性を調べたところ，AAAG配列を有するプロモーターは，エリシター処理により活性化されることが明らかとなった．これらの結果はPsDof１がエリシター応答性防御遺伝子のプロモーター上のAAAG配列に結合し，転写を活性化させる可能性を示唆している．

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Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes.

J. Gen. Plant Pathol.   69: 33-38.   2003年

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DOI： 10.1007/s10327-002-0003-1

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Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes.

J. Gen. Plant Pathol.   69: 33-38.   2003年

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DOI： 10.1007/s10327-002-0003-1

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The DfliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cells.

Mol. Genet. Genomics   269: 21-30.   2003年

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Role of flagella and flagellin in plant-Pseudomonas syrignae interactions. In Japan/ Taiwan Symposium on Molecular Biology of Functional Regulation in Plant and Microbe (N. Sako, H. Yaegashi and M.-K. Yang eds.)

Showado Co. (Saga, Japan)   pp. 158-166.   2003年

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(321)Pseudomonas syringaeの生産する過敏感反応誘導因子(5)P.syringaeの生産するflagellinのタバコに対する過敏感反応誘導特異性(平成14年度 日本植物病理学会大会講演要旨)

田口 富美子, 清水 鈴菜, 竹内 香純, 池田 陽子, 稲垣 善茂, 白石 友紀, 一瀬 勇規

日本植物病理學會報   68 ( 2 )   242 - 243   2002年8月

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記述言語：日本語   出版者・発行元：日本植物病理学会  

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(322)Pseudomonas syringaeの生産する過敏感反応誘導因子(6)P.syringaeのflagellinの糖鎖修飾に関わる推定遺伝子領域の解析(平成14年度 日本植物病理学会大会講演要旨)

竹内 香純, 川崎 敬之, 田口 富美子, 清水 鈴菜, 池田 陽子, 稲垣 善茂, 白石 友紀, 一瀬 勇規

日本植物病理學會報   68 ( 2 )   243 - 243   2002年8月

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記述言語：日本語   出版者・発行元：日本植物病理学会  

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Pseudomonas syringaeの生産する過敏感反応誘導因子(4)べん毛変異体を用いたflagellinの機能解析(関西部会講演要旨)

清水 鈴菜, 田口 富美子, 池田 陽子, 豊田 和弘, 白石 友紀, 稲垣 善茂, 一瀬 勇規

日本植物病理學會報   68 ( 1 )   100 - 100   2002年4月

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記述言語：日本語   出版者・発行元：日本植物病理学会  

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Pseudomonas syringae pv. tabaciの単量体Flagellinは,非宿主植物トマト細胞にHR誘導因子として働く

清水鈴菜, 田口富美子, 池田陽子, 豊田和弘, 白石友紀, 稲垣善茂, 一瀬勇規

日本植物生理学会年会要旨集   42nd   238   2002年3月

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記述言語：日本語  

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HR誘導性細菌エリシターflagellinのHR誘導及び病原性における翻訳後修飾の重要性

田口富美子, 池田陽子, 清水鈴菜, 竹内香純, 稲垣善茂, 白石友紀, 一瀬勇規

日本植物生理学会年会要旨集   42nd   238   2002年3月

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記述言語：日本語  

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cDNA cloning and characterization of tobacco ABC transporter: NtPDR1 is a novel elicitor-responsive gene.

FEBS Letters   518, 164-168   2002年

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DOI： 10.1016/S0014-5793(02)02697-2

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Molecular Cloning of cDNA for a Novel Pea Dof Protein, PsDof1, and Its DNA-Binding Activity to the Promoter of PsDof1 Gene

Seki Hikaru, Nakamura Naoki, Marutani Mizuri, OKABE Takeharu, SANEMATSU Shiroh, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji, ICHINOSE Yuki

Plant biotechnology   19 ( 4 )   251 - 260   2002年

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記述言語：英語   出版者・発行元：Japanese Society for Plant Cell and Molecular Biology  

A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that the GST-PsDof1 recombinant protein bound to specific DNA sequences with an AAAG core. A metal-chelating agent, 1, 10-phenanthroline, abolished binding of GST-PsDof1 to DNA, but the addition of Zn²⁺ restored it, indicating that zinc ions are required for its DNA-binding activity. Interestingly, there are possible sites for binding PsDof1 in its promoter sequence. Indeed, GST-PsDof1 binds to the AAAG-containing promoter sequence of the PsDof1 gene with higher affinity. These results suggest that PsDof1 is a novel transcription factor that is involved in its own transcriptional regulation.

DOI： 10.5511/plantbiotechnology.19.251

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Molecular cloning of cDNA for a novel pea Dof protein, PsDof1, and its DNA binding activity to the promoter of PsDof1 gene.

SEKI H, NAKAMURA N, MARUTANI M, OKABE T, SANEMATSU S, INAGAKI Y, TOYODA K, SHIRAISHI T, ICHINOSE Y

Plant Biotechnol.   19 ( 4 )   251 - 260   2002年

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記述言語：英語   出版者・発行元：Japanese Society for Plant Cell and Molecular Biology  

A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that the GST-PsDof1 recombinant protein bound to specific DNA sequences with an AAAG core. A metal-chelating agent, 1, 10-phenanthroline, abolished binding of GST-PsDof1 to DNA, but the addition of Zn²⁺ restored it, indicating that zinc ions are required for its DNA-binding activity. Interestingly, there are possible sites for binding PsDof1 in its promoter sequence. Indeed, GST-PsDof1 binds to the AAAG-containing promoter sequence of the PsDof1 gene with higher affinity. These results suggest that PsDof1 is a novel transcription factor that is involved in its own transcriptional regulation.

DOI： 10.5511/plantbiotechnology.19.251

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Flagellin, a constituent of a flagella filament in Pseudomonas syringae pv. tabaci, is an intense HR elicitor as a monomer molecule on nonhost tomato cells

R Shimizu, F Taguchi, Y Ikeda, K Toyoda, T Shiraishi, Y Inagaki, Y Ichinose

PLANT AND CELL PHYSIOLOGY   43   S201 - S201   2002年

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：OXFORD UNIV PRESS  

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Cell wall-plasma membrane continuum is involved in transduction of pathogen signals

K Toyoda, AM Kiba, T Kawahara, M Sugimoto, RS Uppalapati, Y Inagaki, Y Ichinose, M Yamamoto, T Shiraishi

PLANT AND CELL PHYSIOLOGY   43   S10 - S10   2002年

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：OXFORD UNIV PRESS  

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The importance of post-translational modification of bacterial HR-elicitor, flagellin on HR-inducing ability and pathogenicity

F Taguchi, Y Ikeda, R Shimizu, K Takeuchi, Y Inagaki, T Shiraishi, Y Ichinose

PLANT AND CELL PHYSIOLOGY   43   S201 - S201   2002年

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：OXFORD UNIV PRESS  

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Biochemical and molecular biological studies on infection (Ascochyta rabiei)-induced thaumatin-like proteins from chickpea plants (Cicer arietinum L.)

T Hanselle, Y Ichinose, W Barz

ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES   56 ( 11-12 )   1095 - 1107   2001年11月

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記述言語：英語   出版者・発行元：VERLAG Z NATURFORSCH  

A pathogenesis-related protein induced by infection with Ascochyta rabiei was purified from intercellular washing fluid of chickpea (Cicer arietinum L.) leaves. Amino-terminal sequencing identified the protein, named PR-5a, as a thaumatin-like protein. The isoelectric point was determined with 6.5 and the molecular mass is 16 kDa. Therefore., chickpea PR-5a is the first dicot member of a TLP subgroup containing small TLPs with a molecular weight between 15 and 18 kDa. PR-5a shows no antifungal activity towards A. rabiei. Screening of a chickpea cDNA library led to the isolation of a cDNA clone (p5a-241) for this protein. A second cDNA clone (ELR112) encoding a TLP was isolated using differential hybridisation of cDNA libraries obtained from elicited and water treated cell suspension cultures of chickpea. The deduced protein (PR-5b) has a molecular mass of 22 kDa. PR-5b is postulated to be located in the vacuole due to the presence of a respective N-terminal signal peptide and a carboxy-terminal extension. Southern blot analyses showed that ELR112 and p5a-241 represent single copy genes. During fungal infection of chickpea plants expression of both genes proceeds much faster in an A. rabiei resistant cultivar than in a susceptible one.

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Generation of hydrogen peroxide is not required for harpin-induced apoptotic cell death in tobacco BY-2 cell suspension culture

Y Ichinose, S Andi, R Doi, R Tanaka, F Taguchi, M Sasabe, K Toyoda, T Shiraishi, T Yamada

PLANT PHYSIOLOGY AND BIOCHEMISTRY   39 ( 9 )   771 - 776   2001年9月

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担当区分：筆頭著者   記述言語：英語   出版者・発行元：GAUTHIER-VILLARS/EDITIONS ELSEVIER  

To characterize molecular and biochemical mechanisms of hypersensitive reaction (HR) in plants, a tobacco suspension culture of BY-2 was treated with the proteinaceous HR elicitor harpin from several pathovars of Pseudomonas syringae. Tobacco BY-2 cells are sensitive to harpins derived from non-pathogenic pathovars of P. syringae, such as pvs. pisi, tomato and glycinea. These three harpins induce apoptotic cell death accompanied by DNA fragmentation in BY-2. Because the cell death was also accompanied by rapid generation of hydrogen peroxide (H2O2), one of the active oxygen species, we investigated the role of H2O2 in harpin-induced cell death. Although treatment with diphenylene iodium chloride (DPI) reduced, and catalase completely abolished, the harpin-induced generation of H2O2, the frequency of cell death was not affected at all. Treatment with superoxide dismutase (SOD) enhanced the generation of H2O2, but the cell death was unaffected. These results indicate that harpin-induced apoptotic cell death does not require oxidative burst. (C) 2001 Editions scientifiques et medicales Elsevier SAS

DOI： 10.1016/S0981-9428(01)01300-6

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Contrary operations of Box-I element of pea phenylalanine ammonia-lyase gene 1 promoter for organ-specific expression

Y Imura, H Seki, K Toyoda, Y Ichinose, T Shiraishi, T Yamada

PLANT PHYSIOLOGY AND BIOCHEMISTRY   39 ( 5 )   355 - 362   2001年5月

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記述言語：英語   出版者・発行元：GAUTHIER-VILLARS/EDITIONS ELSEVIER  

Expression of genes (PSPAL) encoding phenylalanine ammonia-lyase from pea (Pisum sativum L.) is regulated in response to various environmental stimuli and during plant development. We examined the cis-regulatory elements in PSPAL1 promoter for organ-specific expression by determining the sequences specifically associated with nuclear proteins on its promoter in each pea organ. In vivo dimethyl sulfate (DMS) footprinting analysis showed putative protein bindings on AC-rich sequences including Box-I in particular in roots and stems in which PSPAL mRNA were highly accumulated. The potential role of the AC-rich element was investigated by fusing PSPAL1 promoter with Box-I deleted to the reporter gene P-glucuronidase (GUS) and transforming the construct into tobacco plants (Nicotiana tabacum). GUS activity controlled under this Box-I deletion promoter was significantly reduced in roots and leaves, whereas drastically increased in stems as compared with the activity of wild-type PSPALI promoter. Histochemical GUS staining indicated the activity was elevated in vascular tissues of stem by deleting Box-I. These results suggest that Box-I in PSPALI contributes to a positive regulation in root and leaf, but might also function as a negative regulator in stem, especially in xylem tissue. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

DOI： 10.1016/S0981-9428(01)01248-7

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Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J

Y Ichinose, Y Hisayasu, S Sanematsu, Y Ishiga, H Seki, K Toyoda, T Shiraishi, T Yamada

PLANT SCIENCE   160 ( 5 )   997 - 1006   2001年4月

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記述言語：英語   出版者・発行元：ELSEVIER SCI IRELAND LTD  

Clone E86 was isolated as cDNA for elicitor-inducible gene From pea epicotyls by differential screening. The deduced amino acid sequence of E86 showed high homology to hypersensitivity-related protein hsr203J in tobacco and also showed significant homologies to the Ser-active hydrolases, such as mammalian hormone-sensitive lipases, bacterial lipases and esterases. E86 polypeptide possesses consensus amino acid sequence motifs (His-Gly) and (Gly-X-Ser-X-Gly) conserved in lipases and esterases and showed esterase degradation of p-nitrophenyl butyrate. Northern blot analysis revealed that the E86-transcript is abundant in roots and stems and was induced by fungal elicitor in pea epicotyls. However, elicitor-induced accumulation of E86 mRNA was significantly inhibited by the fungal suppressor. Furthermore the expression of the genes encoding E86 and phenylalanine ammonia-lyase was induced within 1 h after the inoculation of a nonpathogen. but it was delayed for 5 h by the inoculation of a compatible pathogen. These results suggest that the elicitor-induced Ser-active hydrolase derived from E86 gene might be related to the plant defense responses. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

DOI： 10.1016/S0168-9452(01)00343-0

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Bacteriophage P4282, a parasite of ┣DB Ralstonia solanacearum (/)-┫DB, encodes a bacteriolytic protein important for lytic infection of its host.

OZAWA H, TANAKA H, ICHINOSE Y, SHIRAISHI T, YAMADA T

Mol. Gen. Genet.   265 ( 1 )   95 - 101   2001年3月

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記述言語：英語  

DOI： 10.1007/s004380000389

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Bacteriophage P4282, a parasite of Ralstonia solanacearum, encodes a bacteriolytic protein important for lytic infection of its host

H Ozawa, H Tanaka, Y Ichinose, T Shiraishi, T Yamada

MOLECULAR GENETICS AND GENOMICS   265 ( 1 )   95 - 101   2001年3月

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記述言語：英語   出版者・発行元：SPRINGER-VERLAG  

To enhance bacterial wilt resistance in tobacco expressing a foreign protein, we isolated the bacteriolytic gene from a bacteriophage that infects Ralstonia solanacearum The bacteriolytic protein of phage P4282 isolated in Tochigi Prefecture was purified from a lysate of R. solanacearum M4S cells infected with the phage, and its bacteriolytic activity was assayed by following the decrease in the turbidity of suspensions of R. solanacearum M4S cells. The molecular weight of the bacteriolytic protein was approximately 71 kDa, and the sequence of the N-terminal 13 amino acids was determined. We used oligonucleotide probes based on this amino acid sequence to isolate the bacteriolytic gene from phage P4282 DNA. This gene of 2061 bp encodes a product of 687 amino acids, whose calaculated molecular weight was 70.12 kDa. The bacteriolytic gene was placed under the control of an inducible promoter, and the plasmid was transformed into Escherichia coli NM522. The soluble proteins extracted from E. coli NM522 cells harboring the plasmid with the bacteriolytic gene showed obvious bacteriolytic activities against several strains of R. solanacearum isolated in various districts in Japan. DNA fragments from five phages, isolated in Niigata. Aomori, Okinawa, Fukushima and Yamaguchi Prefectures, hybridized to the bacteriolytic gene of phage P4282. These observations indicate that the bacteriolytic protein shows nonspecific activity against R. solanacearum strains, and a sequence similar to that of the bacteriolytic gene is conserved in the DNA of other bacteriophages. These results indicate that the generation of transgenic (tobacco) plants expressing the bacteriolytic gene of phage P4282 might result in enhanced resistance to bacterial wilt in tobacco.

DOI： 10.1007/s004380000389

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Harpin_Psta from ┣DB Pseudomonas syringae (/)-┫DB pv.┣DB tabaci (/)-┫DB is defective and deficient in its gene expression and HR-inducing activity.

J. Gen. Plant Pathol.   67 ( 2 )   116 - 123   2001年

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DOI： 10.1007/PL00012997

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Effect of harpin from four pathovars of ┣DB Pseudomonas syringae (/)-┫DB on pea defense responses.

J. Gen. Plant Pathol.   67 ( 2 )   148 - 151   2001年

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DOI： 10.1007/PL00013001

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Effect of harpin from four pathovars of ┣DB Pseudomonas syringae (/)-┫DB on pea defense responses.

TANAKA R, TAGUCHI F, ICHINOSE Y, TOYODA K, SHIRAISHI T, YAMADA T

J. Gen. Plant Pathol.   67 ( 2 )   148 - 151   2001年

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記述言語：英語   出版者・発行元：Phytopathological Society of Japan  

To investigate the role of the proteinaceous elicitor, harpin, on host and nonhost plants, we isolated the harpin-coding gene, hrpZ, from Pseudomonas syringae pvs. pisi, glycinea, tabaci and tomato. Effects of the recombinant harpin proteins on pea plants were analyzed and compared with the effects of the corresponding bacterial treatment. After inoculation of pea with pea pathogen P. syringae pv. pisi, the bacterial population increased and the accumulation of PAL-mRNA and pisatin was inhibited. The nonpathogenic pathovars, glycinea, tabaci and tomato induced both defense responses in pea. However, none of the harpins induced the hypersensitive reaction or accumulation of PAL-mRNA and pisatin in pea. Harpins from P. syringae pvs. glycinea, tomato and pisi did induce these defense responses in tobacco, however, suggesting that externally applied harpins either are not recognized or are nonfunctional in pea plants. (Received July 27, 2000 ; Accepted February 21, 2001)

DOI： 10.1007/PL00013001

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初期・表層シグナル伝達系と防御システム

化学と生物   39 ( 10 )   531 - 537   2001年

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Methyl jasmonate inhibits Harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of defense genes in tobacco suspension cultured BY-2 cells.

Plant Cell Physiol.   42 ( 4 )   446 - 449   2001年

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DOI： 10.1093/pcp/pce056

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Harpin_<psta> from Pseudomonas syringae pv. tabaci Is Defective and Deficient in Its Expression and HR-inducing Activity :

TAGUCHI Fumiko, TANAKA Rui, KINOSHITA Sayuri, ICHINOSE Yuki, IMURA Yoshiyuki, ANDI Salamah, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general plant pathology : JGPP   67 ( 2 )   116 - 123   2001年

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記述言語：英語   出版者・発行元：Phytopathological Society of Japan  

To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P.s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using recombinant harpin indicated harpin_<psta> was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P.s. pathovars in hrp-inducing medium, the truncated harpin_<psta> was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant of the host-parasite specificity in P.s. pv. tabaci. (Received August 10, 2000 ; Accepted December 21, 2000)

DOI： 10.1007/PL00012997

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Gramine increase associated with rapid and transient systemic resistance in barley seedlings induced by mechanical and biological stresses.

Matsuo, H, Taniguchi, K, Hiramoto, T, Yamada, T, Ichinose, Y, Toyoda, K, Takeda, K, Shiraishi, T

Plant Cell Physiol.   42 ( 10 )   1103 - 1111   2001年

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記述言語：英語   出版者・発行元：Japanese Society of Plant Physiologists  

DOI： 10.1093/pcp/pce139

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Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

K Toyoda, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

JOURNAL OF PHYTOPATHOLOGY   148 ( 11-12 )   633 - 636   2000年12月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

When epicotyl tissues of pea were treated with a diacylglycerol (DAG) kinase inhibitor (R59022), enhanced induction of the phytoalexin accumulation occurred which was induced by fungal elicitor. The marked induction was associated with a sustained accumulation of phenylalanine ammonia-lyase (PAL)-mRNA and the consequent increase in PAL activity. These results suggest that inhibition of DAG breakdown leads to increased induction of phytoalexin accumulation and support the hypothesis that DAG kinase negatively regulates the signal transduction.

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Phytoalexin accumulation in elicitor-treated epicotyis of pea(Pisum sativum L.)by a diacylglycerol kinase inhibitor.(共著)

Journal of Phytopathology   148 ( 11-12 )   633 - 636   2000年12月

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記述言語：英語  

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Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture

M Sasabe, K Takeuchi, S Kamoun, Y Ichinose, F Govers, K Toyoda, T Shiraishi, T Yamada

EUROPEAN JOURNAL OF BIOCHEMISTRY   267 ( 16 )   5005 - 5013   2000年8月

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記述言語：英語   出版者・発行元：BLACKWELL SCIENCE LTD  

We characterized pharmacologically the hypersensitive cell death of tobacco BY-2 cells that followed treatments with Escherichia coli preparations of INF1, the major secreted elicitin of the late blight pathogen Phytophthora infestans. INF1 elicitin treatments resulted in fragmentation and 180 bp laddering of tobacco DNA as early as 3 h post-treatment. INF1 elicitin also induced rapid accumulation of H2O2 typical of oxidative burst, and the expression of defense genes such as phenylalanine ammonia-lyase (PAL) gene at 1 h and 3 h after elicitin treatment, respectively. To investigate the involvement of the oxidative burst and/or the expression of defense genes in the signal transduction pathways leading to hypersensitive cell death, we analyzed the effect of several chemical inhibitors of signal transduction pathways on the various responses. The results indicated that (a) the cell death required serine proteases, Ca2+ and protein kinases, (b) the oxidative burst was involved in Ca2+ and protein kinase mediated pathways, but elicitin-induced AOS was neither necessary nor sufficient for cell death and PAL gene expression, and (c) the signaling pathway of PAL gene expression required protein kinases. These results suggest that the three signal transduction pathways leading to cell death, oxidative burst and expression of defense genes branch in the early stages that follow elicitin recognition by tobacco cells.

DOI： 10.1046/j.1432-1327.2000.01553.x

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Characterization of cDNAs encoding two glycine-rich proteins in chickpea (Cicer arietinum L.) : Accumulation in response to fungal infection and other stress factors.(共著)

CORNELS H, ICHINOSE Y, BARZ W

Plant Science   154 ( 1 )   83 - 88   2000年5月

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記述言語：英語  

DOI： 10.1016/S0168-9452(00)00193-X

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Cytochalasin A inhibits the binding of phenylalanine ammonia-lyase mRNA to ribosomes during induction of phytoalexin in pea seedlings

M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY   41 ( 2 )   234 - 238   2000年2月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

Cytochalasin A (CA) blocked the accumulation of phytoalexin and phenylalanine ammonia-lyase (PAL)-protein in pea tissues treated with a fungal elicitor but scarcely affected the PAL-mRNA content. Further analysis showed that CA decreased the PAL-mRNA bound to ribosomes. These results indicate that actin filaments are tightly associated with the translational process of the PAL gene.

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Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization.(共著)

Zeitschrift fuer Naturforschung   55 ( 1-2 )   44 - 54   2000年1月

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記述言語：英語  

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lmportance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression lnduced by Nonpathogen Attack.(共著)

IMURA Y, IGUCHI S, TOYODA K, ICHINOSE Y, SHIRAISHI T, YAMADA T

Journal of General Plant Pathol.ogy   66 ( 2 )   123 - 127   2000年

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記述言語：英語   出版者・発行元：Phytopathological Society of Japan  

Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

DOI： 10.1007/PL00012932

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Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

K. Toyoda, T. Kawahara, Y. Ichinose, T. Yamada, T. Shiraishi

Journal of Phytopathology   148 ( 11-12 )   633 - 636   2000年

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記述言語：英語  

When epicotyl tissues of pea were treated with a diacylglycerol (DAG) kinase inhibitor (R59022), enhanced induction of the phytoalexin accumulation occurred which was induced by fungal elicitor. The marked induction was associated with a sustained accumulation of phenylalanine ammonia-lyase (PAL)-mRNA and the consequent increase in PAL activity. These results suggest that inhibition of DAG breakdown leads to increased induction of phytoalexin accumulation and support the hypothesis that DAG kinase negatively regulates the signal transduction.

DOI： 10.1046/j.1439-0434.2000.00568.x

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Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (┣DB Pisum sativum (/)-┫DB L.) by a diacylglycerol kinase inhibitor.

J. Phytopathol.   148 ( 11-12 )   633 - 636   2000年

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記述言語：英語  

DOI： 10.1046/j.1439-0434.2000.00568.x

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lndependent pathways leading to apoptotic cell death,oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture.(共著)

SASABE M, TAKEUCHI K, ICHINOSE Y, TOYODA K, SHIRAISHI T, YAMADA T, KAMOUN S, GOVERS F

European Journal of Biochemistry   267 ( 16 )   5005 - 5013   2000年

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DOI： 10.1046/j.1432-1327.2000.01553.x

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Cytochalasin A inhibits the binding of phenylalanine ammonia-lyass mRNA to ribosomes during induction of phytoalexin in pea seedlings.(共著)

Plant Cell Physiology   41   234 - 238   2000年

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Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack :

IMURA Yoshiyuki, IGUCHI Satoko, TOYODA Kazuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general plant pathology : JGPP   66 ( 2 )   123 - 127   2000年

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記述言語：英語   出版者・発行元：Phytopathological Society of Japan  

Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

DOI： 10.1007/PL00012932

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その他リンク： http://id.nii.ac.jp/1141/00138133/

PLANT CELL WALL RECOGNIZES AND RESPONDS TO SIGNALS FROM PHYTOPATHOGENS :

KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology   41   s10   2000年

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記述言語：英語   出版者・発行元：Japanese Society of Plant Physiologists  

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STRUCTURAL AND FUNCTIONAL ANALYSES OF HARPINS FROM SEVERAL PATHOVARS OF PSEUDOMONAS SYRINGAE :

TANAKA Rui, TAGUCHI Tomiko, KINOSHITA Sayuri, NAKADA Hideki, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology   41   s84   2000年

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記述言語：英語   出版者・発行元：Japanese Society of Plant Physiologists  

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植物病原菌と防御機構 (環境応答・適応の分子機構) -- (植物の環境応答と耐性のメカニズム)

山田 哲治, 白石 友紀, 一瀬 勇規

蛋白質核酸酵素   44 ( 15 )   2308 - 2314   1999年11月

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記述言語：日本語   出版者・発行元：共立出版  

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Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes

A Kiba, T Takeda, T Kanemitsu, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY   40 ( 9 )   978 - 985   1999年9月

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記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

A high molecular weight elicitor (&gt;70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of beta-D-Glc-(1--&gt;6)-alpha-D-Man-(1--&gt;6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including beta-D-Glc-(1--&gt;6)-alpha-D-Man-(1--&gt;6)-D-Man-O-Ser (No. 1) to [beta-D-Glc-(1--&gt;6)-alpha-D-Man-(1--&gt;6)-D-Man](3)-O-Ser(3)-Pro(3) (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycopeptides [beta-D-Glc-(1--&gt;6)-alpha-D-Man-(1--&gt;6)-D-Man]-O-Ser(2)-Pro(2) (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

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Functional analysis of retrotransposons in pea

H Kato, P Sriprasertsak, H Seki, Y Ichinose, T Shiraishi, T Yamada

PLANT AND CELL PHYSIOLOGY   40 ( 9 )   933 - 941   1999年9月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

The 5'-upstream regions of the plant active defense genes in pea (PSPAL2 and PSCHS1) exhibit significant nucleotide sequence identity to part of a copia-type retrotransposon. To characterize the retrotransposon in pea putative reverse transcriptase sequences (Psr) were amplified by PCR from cDNA prepared from protoplasts derived from pea suspension cultured cells. Psr genes can be classified into at least eight subgroups (A-H) according to their nucleotide sequence similarities. A subgroup PsrC was induced by the treatment of etiolated pea epicotyls with fungal elicitor within 30 min but was hardly induced by protoplasting of pea suspension cultured cells, whereas subgroups of PsrA and PsrB were highly induced by protoplasting. Interestingly, the retrotransposon sequence present at the 5'-upstream region of PSCHS1 which was induced by the treatment with fungal elicitor belonged to subgroup PsrC. The retrotransposon might play an important role in reorganization of the genes and optimization of the gene expression in response to various environmental stimuli such as an attack by phytopathogens or protoplasting.

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Changes in in vivo DNA-protein interactions in pea phenylalanine ammonia-lyase and chalcone synthase gene promoter induced by fungal signal molecules

H Seki, Y Nagasugi, Y Ichinose, T Shiraishi, T Yamada

PLANT AND CELL PHYSIOLOGY   40 ( 1 )   88 - 95   1999年1月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

Elicitor-induced protein bindings were detected on the several particular sequence motifs in two members of the pea PAL and CHS gene promoters by in vivo footprinting analyses. However, elicitor-induced changes rapidly reverted back to the uninduced pattern after the subsequent application of suppressor from M. pinodes.

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Changes in in vivo DNA-potein interactions in pea phenylalanine ammonia-lyase and chalcone synthase gene promoter induced by fungal signal molecules.(共著)

Plant Cell Physiology   40 ( 1 )   88 - 95   1999年1月

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記述言語：英語  

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Molecular cloning of a cDNA encoding a putative DNA-binding zinc-finger protein in pea.(共著)

Scientific Reports of the Faculty of Agriculture Okayama University   88   25 - 30   1999年

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Expression of the chimeric pea PSPAL2 promoter in transgenic tobacco in response to fungal ingress and injury.(共著)

Permpong SRIPRASERTSAK, Andi SALAMAH, Yoshiyuki IMURA, Yuki ICHINOSE, Tomonori SHIRAISHI, Tetsuji YAMADA

Annals of Phytopathological Society of Japan   65 ( 2 )   123 - 130   1999年

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DOI： 10.3186/jjphytopath.65.123

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Expression of the chimeric pea PSPAL2 promoter in transgenic tobacco in response to fungal ingress and injury.(共著)

井村 善之, 一瀬 勇規, 白石 友紀, 山田 哲治

Annals of Phytopathological Society of Japan   65 ( 2 )   123 - 130   1999年

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記述言語：英語   出版者・発行元：日本植物病理学会  

5'-PAL遺伝子プロモータ上流領域(-2196〜+110)を順次削り取り, GUSレポーター遺伝子に連結したキメラ遺伝子をタバコに導入後, 4種PSPAL2-FL(-2196〜+l10), PSPAL2-FLd1(-1486〜+110), PSPAL2-FLd2(-966〜+110), PSPAL2-FLd3(-594〜+110)のトランスジェニック植物体を作出した。トランスジェニッククバコ葉におけるGUS発現様式を組織学的に観察したところ, タバコの非病原菌(Phytophthora capsici)接種において, PSPAL2-FLおよびPSPAL2-FLd1導入体では, 接種2日目で過敏感反応を起こした組織の周辺細胞できわめて顕著なGUS発現が検出されたのに対し, 病原菌(Phytophthora nicotiana)接種葉では, 微弱なGUS発現が接種部位周辺に拡がって検出された。また, 剃刀でタバコ葉に付傷した場合にも顕著なGUS発現がみられた。これらの結果は, PSPAL2の遺伝子発現は病原菌接種に限らず, 傷によっても誘導されること, 本発現にはプロモータ上流域(-2196〜ー966)が必要であることを示している。

DOI： 10.3186/jjphytopath.65.123

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Cloning and Structural Characterization of hrp Locus of Pseudomonas syringae pv. pisi.(共著)

Hideki NAKADA, Aiko TAHARA, Masaharu HAYASHI, Rena SHIMIZU, Rui TANAKA, Yuki ICHINOSE, Tomonori SHIRAISHI, Tetsuji YAMADA

Annals of Phytopathological Society of Japan   65 ( 2 )   147 - 152   1999年

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DOI： 10.3186/jjphytopath.65.147

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Transformation of the Endophyte Neotyphodium with the iaaM Gene.

YUNUS Ahmad, 川又 伸治, 島貫 忠幸, 村上 泰弘, 一瀬 勇規, 白石 友紀, 山田 哲治

日本植物病理学会報   65 ( 2 )   192 - 196   1999年

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記述言語：英語   出版者・発行元：The Phytopathological Society of Japan  

オリーブこぶ病細菌のiaaM遺伝子(トリプトファンモノオキシゲナーゼ遺伝子)をネオティフォディウム・エンドファイト菌に導入し形質転換を試みた。その結果,ポリエチレングリコール(PEG)を用いる方法がエレクトロポレーション法に比べて高い形質転換効率を示した。ネオティフォディウムTF91006株とTi91093株の形質転換体におけるiaaM遺伝子と薬剤耐性マーカーのハイグロマイシン耐性遺伝子の染色体DNAへの組込みは,PCR法によって予想される560bpのiaaM遺伝子と1.7kbのハイグロマイシン耐性遺伝子の増幅によって確認した。これらの形質転換体におけるiaaM遺伝子の発現を培養ろ液中に蓄積されたインドールアセトアミド(IAM)量をHPLCによって定量することによって調べたところ,多くの形質転換体では有意なIAMの蓄積が検出された。

DOI： 10.3186/jjphytopath.65.192

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Tranformation of the endophyte Neotyphodium With the iaaM gene(共著)

川又 伸治, 島貫 忠幸, 村上 泰弘, 一瀬 勇規, 白石 友紀, 山田 哲治

Annals of Phytopathological Society of Japan   65 ( 2 )   192 - 196   1999年

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記述言語：英語   出版者・発行元：The Phytopathological Society of Japan  

オリーブこぶ病細菌のiaaM遺伝子(トリプトファンモノオキシゲナーゼ遺伝子)をネオティフォディウム・エンドファイト菌に導入し形質転換を試みた。その結果,ポリエチレングリコール(PEG)を用いる方法がエレクトロポレーション法に比べて高い形質転換効率を示した。ネオティフォディウムTF91006株とTi91093株の形質転換体におけるiaaM遺伝子と薬剤耐性マーカーのハイグロマイシン耐性遺伝子の染色体DNAへの組込みは,PCR法によって予想される560bpのiaaM遺伝子と1.7kbのハイグロマイシン耐性遺伝子の増幅によって確認した。これらの形質転換体におけるiaaM遺伝子の発現を培養ろ液中に蓄積されたインドールアセトアミド(IAM)量をHPLCによって定量することによって調べたところ,多くの形質転換体では有意なIAMの蓄積が検出された。

DOI： 10.3186/jjphytopath.65.192

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Functional Analysis of retrotransposons in pea.(共著)

Plant Cell Phyiology   40   933 - 941   1999年

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Cloning and Structural Characterization of hrp Locus of Pseudomonas syringae pv. pisi.(共著)

中田 秀毅, 田原 愛子, 林 昌治, 清水 鈴菜, 田中 塁, 一瀬 勇規, 白石 友紀, 山田 哲治

Annals of Phytopathological Society of Japan   65 ( 2 )   147 - 152   1999年

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記述言語：英語   出版者・発行元：日本植物病理学会  

エンドウつる枯れ病細菌(Pseudomonas Syringae pv. pisi)レース1のhrp遣伝子座をSuperCoslベクターにクローニングすると同時に, レース2〜4はレース1の塩基配列を参考としてPCR産物を調製し, pBluescript SK(+)にクローニングした後, hrpZの塩基配列から決定したアミノ酸配列を比較した。Pseudomonas 属のHrpZ (ハーピン)のアミノ酸配列では95〜50%の相同性があったが, Erwiniaではさらに相同性が低かった。しかし, レース1, 2, 3のHrpZのアミノ酸配列の相同性は99%以上と高かった。P. s. pisiのHrpZタンパク質は他のハーピンと同様, グリシンに富み熱に比較的安定で, カルボキシル末端にGGGLGTPとQTGTのダイレクトリピートを保持していた。また, 興味深いことにエンドウ褐紋病菌のサプレッシンBの6個のペプチドシークエンスのうち, 4残基の配列相同性がHrpZに存在していた。

DOI： 10.3186/jjphytopath.65.147

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cNDA cloning and gene expression of three small GTP-binding proteins in defense response of chickpea.(共著) 査読

Biochimica et Biophysica Acta   1489 ( 2-3 )   462 - 466   1999年

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：速報，短報，研究ノート等（学術雑誌）  

DOI： 10.1016/S0167-4781(99)00201-8

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植物病原菌と防御機構(共著)

蛋白質・核酸・酵素   44   2308 - 2314   1999年

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Molecular cloning of a cDNA encoding a putative DNA-binding zinc-finger protein in pea.(共著)

Scientific Reports of the Faculty of Agriculture Okayama University   88   25 - 30   1999年

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Co-purification of plasma membrane ATPase and phosphatidylinositol kinase from pea plasma membrane.(共著)

Scientific Reports of the Faculty of Agriculture Okayama University   88   31 - 38   1999年

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Co-purification of plasma membrane ATPase and phosphatidylinositol kinase from pea plasma membrane.(共著)

Scientific Reports of the Faculty of Agriculture Okayama University   88   31 - 38   1999年

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Inductions of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes.(共著)

Plant Cell Phyiology   40   978 - 985   1999年

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トマト青枯病細菌Ralstonia solanacearumの非病原化プラスミドpJTPS1の構造解析

清水 鈴菜, 赤石 維衆, 根岸 秀明, 田中 博, 一瀬 勇規, 白石 友紀, 山田 哲治

日本植物病理學會報   65 ( 2 )   184 - 188   1999年

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記述言語：英語   出版者・発行元：日本植物病理学会  

トマト青枯病細菌の病原性野性株U-7から分離された非病原性突然変異体M4Sに特異的に存在しているプラスミドpJTPS1の全塩基配列を決定した。本プラスミドの塩基配列なびに推定ORFの相同性を検索した結果, Pseudomonas aeruginosaのバクテリオファージPf3のORF301や病原性および非病原性因子として報告されているトマト青枯病細菌のPopAl, HrpFの一部と相同性が検出された。サザンブロット解析によって, U-7のゲノム上にpJTPS1様配列が存在したこと, 本pJTPS1様配列はPopA1相同配列の近傍でpJTPS1非相同配列に連続していたことから, pJTPS1はU-7ゲノム上のpJTPS1様配列が切り出され環状化されたプラスミドであり, 本プラスミドの出現が非病原性に関与している可能性が推定された。

DOI： 10.3186/jjphytopath.65.184

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Structural analysis of a putative hypovirulent plasmid, pJTPS1, found in a spontaneous avirulent mutant of Ralstonia solanacearum.(共著)

清水 鈴菜, 赤石 維衆, 根岸 秀明, 田中 博, 一瀬 勇規, 白石 友紀, 山田 哲治

Annals of Phytopathological Society of Japan   65 ( 2 )   184 - 188   1999年

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記述言語：英語   出版者・発行元：日本植物病理学会  

トマト青枯病細菌の病原性野性株U-7から分離された非病原性突然変異体M4Sに特異的に存在しているプラスミドpJTPS1の全塩基配列を決定した。本プラスミドの塩基配列なびに推定ORFの相同性を検索した結果, Pseudomonas aeruginosaのバクテリオファージPf3のORF301や病原性および非病原性因子として報告されているトマト青枯病細菌のPopAl, HrpFの一部と相同性が検出された。サザンブロット解析によって, U-7のゲノム上にpJTPS1様配列が存在したこと, 本pJTPS1様配列はPopA1相同配列の近傍でpJTPS1非相同配列に連続していたことから, pJTPS1はU-7ゲノム上のpJTPS1様配列が切り出され環状化されたプラスミドであり, 本プラスミドの出現が非病原性に関与している可能性が推定された。

DOI： 10.3186/jjphytopath.65.184

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Plant pathogens and mechanisms of defense responses

T. Yamada, T. Shiraishi, Y. Ichinose, K. Toyoda

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   44 ( 15 )   2308 - 2314   1999年

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記述言語：日本語   掲載種別：書評論文，書評，文献紹介等  

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Interaction between cell wall and plasma membrane via RGD motif is implicated in plant defense response.(共著)

Plant Cell Physiology   39 ( 11 )   1245 - 1249   1998年11月

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記述言語：英語  

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Interaction between cell wall and plasma membrane via RGD motif is implicated in plant defense responses

A Kiba, M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY   39 ( 11 )   1245 - 1249   1998年11月

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記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

Integrin- and vitronectin-like proteins were found to exist in the pea plasma membrane and cell wall, respectively. The hexapeptide GRGDSP but not GRGESP inhibited either the binding of cell wall protein(s) to plasma membrane protein(s) or the defense response. A possible role of linkage via RGD motif in plant defenses is discussed.

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SPECIFIC REGULATION OF CELL WALL-BOUND ATPASE BY THE SUPPRESSOR FROM MYCOSPHAERELLA PINODES(3) -IDENTIFICATION OF ELICITOR- AND SUPPRESSOR-BINDING PROTEINS IN CELL WALL-BOUND ATPASE FRACTION FROM PEA PLANT-

KIBA Akinori, SUGIMOTO Megumi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology   39   S144 - S144   1998年5月

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記述言語：英語  

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ISOLATION AND CHARACTERIZATION OF ELICITOR-RESPONSIVE GENES IN PEA

ICHINOSE Yuki, SANEMATSU Shiroh, ENDOH Ai, OKABE Takeharu, HISAYASU Yumiko, SEKI Hikaru, SHIRAISHI Tomonori, TOYODA Kazuhiro, YAMADA Tetsuji

Plant and cell physiology   39   S143 - S143   1998年5月

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記述言語：英語  

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In vivo FOOTPRINTING ANALYSIS OF cis-REGULATORY ELEMENTS INVOLVED IN THE ELICITOR-MEDIATED ACTIVATION AND SUPPRESSOR-MEDIATED DEACTIVATION OF PEA PAL AND CHS

SEKI Hikaru, ICHINOSE Yuki, NAGASUGI Yumi, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology   39   S43 - S143   1998年5月

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記述言語：英語  

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RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS II

SUGIURA Tetsuya, KIBA Akinori, AOYAGI Masaaki, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology   39   S144 - S144   1998年5月

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PURIFICATION AND ANALYSIS OF ELICITOR BINDING-PROTEIN IN CELL WALL FRACTION FROM PEA

SUGIMOTO Megumi, KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology   39   S143 - S143   1998年5月

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Cis-regulatory elements and trans-acting factors involved in the activation of a member of elicitor-responsive pea chalcone synthase gene family, PSCHS2(共著)

Scientific Reports of the Faculty of Agriculture Okayama University   87   91 - 97   1998年

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病原菌シグナルによるエンドウ原形質膜におけるホスファチジルイノシトールリン脂質のリン酸化とリゾリン脂質生成の制御

Toyoda Kazuhiro, Koyama Masashi, Mizukoshi Rumi, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

岡山大学農学部学術報告   87 ( 1 )   109 - 116   1998年

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記述言語：英語   出版者・発行元：岡山大学農学部  

Effects of elicitor and suppressor from a pea pathogen, Mycosphaerella pinodes, on Pl etabolism in pea plasma membrane were examined in vitro. The elicitor induced rapid phosphorylation of phosphatidylinositols as well as production of lysophospholipid in plasma membranes, but these responses were severely inhibited by the suppressor. These results indicate that a membrane-associated phospholipase A is regulated coordinately by fungal signals, together with Pl metabolism, and that it may participate in signal transduction pathways leading to defense responses. To evaluate a possible rote of phospholipase A activation in induction of a pea defense response, the effect of free fatty acid on induction of a phytoalexin accumulation was also examined. When pea leaves were treated with linoleic- or linolenic acid, most commonly released in plant cells by phospholipase A, the accumulation of pisatin was induced even in the absence of the elicitor. It is, therefore, conceivable that free fatty acid(s) released from plasma membrane is also implicated in the early stage of elicitor-signal transduction in pea.エンドウの上胚軸組織により分離した原形質膜画分を褐紋病菌の生産するエリシターで処理すると、ホスファチジルイノシトールリン脂質の急速なリン酸化とリゾリン脂質の生成が誘導されたが、同菌より調製したサプレッサーの共存下では双方とも著しく阻害された。本結果は、ポリホスホイノシチド代謝系と同調的に作動するホリパーゼA活性化が存在すること、さらに、原形質膜における病原菌シグナルの受容・応答には複数の資質代謝系が介在する可能性を示唆している。一方、ホスホリパーゼAの活性化の役割を調べる目的で、本酵素によって原形質膜から生成されると考えられる脂肪酸(リノール酸ならびにリノレン酸)をエンドウ葉に処理したところ、エリシターの非存在下においてもファイトアレキシンであるピサチンの生成が誘導されることが示された。以上から、ポリホスホイノシチド代謝系と同調的に働くホスホリパーゼAがエリシターシグナルの初期伝達に深く関連しているものと考えられた。

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Cis-regulatory elements and trans-acting factors involved in the activation of a member of elicitor-responsive pea chalcone synthase gene family, PSCHS2(共著)

Scientific Reports of the Faculty of Agriculture Okayama University   87   91 - 97   1998年

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Transformation of mutualistic fungal Acremonium endophyte(共著)

Scientific Reports of the Faculty of Agriculture Okayama University   87   99 - 107   1998年

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Phosphorylation of phosphatidylinositols and production of lysophospholipid in pea plasma membrane are coordinately regulated by elicitor-and suppressor from Mycosphaerella pinodes(共著)

Toyoda Kazuhiro, Koyama Masashi, Mizukoshi Rumi, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

Scientific Reports of the Faculty of Agriculture Okayama University   87 ( 1 )   109 - 116   1998年

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記述言語：英語   出版者・発行元：岡山大学農学部  

Effects of elicitor and suppressor from a pea pathogen, Mycosphaerella pinodes, on Pl etabolism in pea plasma membrane were examined in vitro. The elicitor induced rapid phosphorylation of phosphatidylinositols as well as production of lysophospholipid in plasma membranes, but these responses were severely inhibited by the suppressor. These results indicate that a membrane-associated phospholipase A is regulated coordinately by fungal signals, together with Pl metabolism, and that it may participate in signal transduction pathways leading to defense responses. To evaluate a possible rote of phospholipase A activation in induction of a pea defense response, the effect of free fatty acid on induction of a phytoalexin accumulation was also examined. When pea leaves were treated with linoleic- or linolenic acid, most commonly released in plant cells by phospholipase A, the accumulation of pisatin was induced even in the absence of the elicitor. It is, therefore, conceivable that free fatty acid(s) released from plasma membrane is also implicated in the early stage of elicitor-signal transduction in pea.エンドウの上胚軸組織により分離した原形質膜画分を褐紋病菌の生産するエリシターで処理すると、ホスファチジルイノシトールリン脂質の急速なリン酸化とリゾリン脂質の生成が誘導されたが、同菌より調製したサプレッサーの共存下では双方とも著しく阻害された。本結果は、ポリホスホイノシチド代謝系と同調的に作動するホリパーゼA活性化が存在すること、さらに、原形質膜における病原菌シグナルの受容・応答には複数の資質代謝系が介在する可能性を示唆している。一方、ホスホリパーゼAの活性化の役割を調べる目的で、本酵素によって原形質膜から生成されると考えられる脂肪酸(リノール酸ならびにリノレン酸)をエンドウ葉に処理したところ、エリシターの非存在下においてもファイトアレキシンであるピサチンの生成が誘導されることが示された。以上から、ポリホスホイノシチド代謝系と同調的に働くホスホリパーゼAがエリシターシグナルの初期伝達に深く関連しているものと考えられた。

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Transformation of mutualistic fungal Acremonium endophyte(共著)

Scientific Reports of the Faculty of Agriculture Okayama University   87   99 - 107   1998年

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Elevation of diacylglycerol during the early stage of elictitor-signal transduction in pea(Pisum sativum).(共著)

Kazuhiro TOYODA, Tomoharu KAWAHARA, Rumi MIZUKOSHI, Masashi KOYAMA, Yuki ICHINOSE, Tetsuji YAMADA, Tomonori SHIRAISHI

Annals of Phytopathological Society of Japan   64 ( 5 )   485 - 487   1998年

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DOI： 10.3186/jjphytopath.64.485

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Elevation of diacylglycerol during the early stage of elictitor-signal transduction in pea(Pisum sativum).(共著)

豊田 和弘, 河原 智治, 水越 留美, 小山 昌史, 一瀬 勇規, 山田 哲治, 白石 友紀

Annals of Phytopathological Society of Japan   64 ( 5 )   485 - 487   1998年

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記述言語：英語   出版者・発行元：日本植物病理学会  

エリシターの認識とそのシグナル伝達機構を解析する目的で, エンドウ上胚軸組織におけるジアシルグリセロール生成量を調べた。この結果, エリシター処理組織においては, 処理後1分以内に急速なジアシルグリセロールの生成が認められたが, ホスホリパーゼCの阻害剤であるネオマイシンやエンドウ褐紋病菌のサプレッサーの共存下には, その生成は著しく阻害された。この結果は, 細胞内におけるイノシトール3リン酸を定量した既報の結果と一致し, ホスホリパーゼC活性の一過的上昇がエリシターシグナルの伝達に必須であることを強く示唆する。このことは, 細胞内のジアシルグリセロール濃度の上昇を引き起こすジアシルグリセロールキナーゼ阻害剤(R59022)で処理された組織では, エリシターの処理によって誘導されるファイトアレキシンの生成量が約4倍に増加することからも支持された。

DOI： 10.3186/jjphytopath.64.485

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その他リンク： http://agriknowledge.affrc.go.jp/RN/2010581036

植物細胞壁が病原菌を認識する

白石 友紀, 木場 章範, 豊田 和弘, 一瀬 勇規, 山田 哲治

化学と生物   36 ( 4 )   226 - 233   1998年

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記述言語：日本語   出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/kagakutoseibutsu1962.36.226

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Functional Analysis of the Putative cis-elements Involved in Activation by Elicitor and Deactivation by Suppressor in the Promoter of a Pea Gene for Phenylalanine Ammonia-1yase(PSPAL1).(共著)

Plant Cell Physiology   38 ( 12 )   1403 - 1408   1997年12月

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記述言語：英語  

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Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules.(共著)

KIBA A, MIYAKE C, TOYODA K, ICHINOSE Y, YAMADA T, SHIRAISHI T

Phytopathology   87 ( 8 )   846 - 852   1997年8月

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記述言語：英語  

DOI： 10.1094/PHYTO.1997.87.8.846

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Temporal and spatial pattern of expression of the pea phenylalanine ammonia-lyase gene1 promoter in transgenic tobacco

S Kawamata, K Shimoharai, Y Imura, M Ozaki, Y Ichinose, T Shiraishi, H Kunoh, T Yamada

PLANT AND CELL PHYSIOLOGY   38 ( 7 )   792 - 803   1997年7月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

Genes encoding phenylalanine ammonia-lyase (PAL) form a small multigene family with at least three members in pea. Tissue-specific expression of the promoter of a member of PAL gene family (PSPAL1) was investigated in the transgenic tobacco transformants carrying the different modes of chimeric fusion between the PSPAL1 promoter and a bacterial beta-glucuronidase (GUS) gene. In stems, at least, strict correlation was found between steady-state levels of Gus-mRNA and enzyme activity. Significantly high level of GUS activity was observed in roots, particularly in meristematic tissues and the pigmented region of petals of transgenic tobacco carrying the translational fusion type B (-1,394 to +140 of PSPAL1 connected to Gus), followed by moderately high level of GUS activity carrying the translational fusion type A(-1,394 to +117). GUS expression in tissues of mature leaves, however, was very low in these constructs. Extremely low GUS activity was observed in the transformants of transcriptional fusion type (-1,394 to +5), whilst no activity was detected carrying non-transcription fusion type (-1,394 to -27). Furthermore, the pattern of the PSPAL1 expression was characterized in response to pathogen ingress and woundings in transgenic tobacco carrying the translational fusion type B. Woundings itself triggered marked expression of PSPAL1-driven GUS expression at the wounded sites. Inoculation of nonpathogens, Phytophthora capsici, P. boehmeriae and Erisiphe graminis f. sp. hordei, both caused rapid and very clear GUS expression zone along with the development of hypersensitive cell death area where callose was accumulated, however, the inoculation of a pathogen, P. nicotiana caused slow and hazy GUS expression zone along with the lesion development. These results suggest that the expression of pea PSPAL1 promoter is regulated in a similar fashion, at least in a part, in pea and transgenic tobacco, under the plant development and various environmental cues.

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Putative cis-regulatory elements for elicitor-mediated transcriptional activation and molecular evolution of the pea chalcone synthase genes.

Y Ichinose, H Seki, M Ito, T Shiraishi, T Yamada

PLANT PHYSIOLOGY   114 ( 3 )   1175 - 1175   1997年7月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER SOC PLANT PHYSIOLOGISTS  

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Specific regulation of cell wall-bound ATPase and peroxidase by the suppressor for defense response.

A Kiba, A Inata, C Miyake, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT PHYSIOLOGY   114 ( 3 )   1176 - 1176   1997年7月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER SOC PLANT PHYSIOLOGISTS  

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Con A-binding protein in plasma membranes may participate in signal-transduction cascades that lead to defense responses in pea.

K Toyoda, M Sugimoto, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

PLANT PHYSIOLOGY   114 ( 3 )   1502 - 1502   1997年7月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER SOC PLANT PHYSIOLOGISTS  

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Association between ion fluxes and defense responses in pea and cowpea tissues

M Amano, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY   38 ( 6 )   698 - 706   1997年6月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

The glycopeptide elicitor from a pea pathogen, Mycosphaerella pinodes, induced rapid alkalinization and increases in levels of Na+ and K+ ions in the extracellular solution upon contact with pea and cowpea tissues, The presence of monensin, nigericin, lidocaine, quinidine or phenytoin together with the elicitor markedly inhibited these changes, whereas the presence of valinomycin, gramicidin D, tetraethylammonium, CsCl and aminopyridine did not. The production of phytoalexins in pea and cowpea tissues was also strongly inhibited by the simultaneous presence of the former reagents but not of the latter reagents. Inhibitory effects on the production of phytoalexins were diminished when monensin, nigericin or a Na+-channel blocker was applied 3 h after the start of treatment with elicitor, Furthermore, orthovanadate and neomycin, which suppress defense responses in both tissues, also inhibited the above mentioned changes. By contrast, the species-specific suppressor from M. pinodes inhibited the elicitor-induced release of Na+ and K+ ions from pea tissues, but, conversely, by itself it elicited either the defense response or the release of Na+ and K+ ions from cowpea tissues. The results indicate that these ion-related changes, in particular the efflux of Na+ and K+ ions, might be closely associated with the signal transduction system for defense responses at the tissue level.

DOI： 10.1093/oxfordjournals.pcp.a029223

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Molecular evolution and functional relevance of the chalcone synthase genes of pea

M Ito, Y Ichinose, H Kato, T Shiraishi, T Yamada

MOLECULAR & GENERAL GENETICS   255 ( 1 )   28 - 37   1997年6月

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記述言語：英語   出版者・発行元：SPRINGER VERLAG  

We have isolated seven genomic chalcone synthase (CHS) genes and six classes of CNS cDNA from elicitor-treated pea tissues. Comparison of the nucleotide sequences of the coding regions revealed the existence of eight members of the CHS gene family in pea. These can essentially be divided into three groups (PSCHS1, 2 and 8; PSCHS3, 4 and 5; and PSCHS6 and 7) on the basis of nucleotide and/or amino acid sequence comparisons of the coding regions, introns and promoter regions. We previously reported that the accumulation of CHS mRNAs is induced by elicitor treatment. Accumulation of CHS mRNA was observed mainly in roots and very little was found in floral organs. To specifically detect expression of each CHS gene in various types of pea cells, S1 nuclease protection assays were performed. Interestingly, the classification of the eight members of the CHS gene family based on the sequence identity was found to reflect their expression patterns as determined by the S1 nuclease protection assay. The first group of CHS genes, PSCHS1, 2 and 8, was strongly induced not only by elicitor treatment and UV irradiation but is also constitutively expressed in root and flower tissues. The second group, PSCHS3, 4 and 5, was also strongly induced by elicitor treatment and UV irradiation but is constitutively expressed only in root. Expression of the third group, PSCHS6 and 7 was barely detectable in any of the organs tested and was not influenced by environmental stimuli such as elicitor or UV. Furthermore, sequence analysis of the promoter region of each member of the CHS gene family revealed that putative cis-regulatory elements, such as Box-I, Box-II and G-Box, were conserved only in PSCHS1, 2, 3, 4 and 5. From these results we propose that an ancestral CHS gene might have given rise to defense response-related (UV irradiation- and elicitor-responsive) and -unrelated (unresponsive) genes at an early stage of evolution, followed by divergence within these subclasses based upon the developmental program in pea.

DOI： 10.1007/s004380050471

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dad-1, a putative programmed cell death suppressor gene in rice

Y Tanaka, T Makishima, M Sasabe, Y Ichinose, T Shiraishi, T Nishimoto, T Yamada

PLANT AND CELL PHYSIOLOGY   38 ( 3 )   379 - 383   1997年3月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

The human dad-1 cDNA homolog was isolated from rice plants. The amino acid sequence of the predicted protein product is well conserved in both animals and plants. This rice dad-1 homolog can rescue the temperature-sensitive dad-1 mutants of hamster cells from apoptotic death, suggesting that the rice dad-1 homolog also functions as a suppressor for programmed cell death.

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RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS -SIGNIFICANCE OF CELL WALL-

SUGIURA Tetsuya, KIBA Akinori, AOYAGI Masaaki, KATO Toshiaki, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology   38   s58   1997年3月

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記述言語：英語  

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THE SIGNIFICANCE OF ACTIVE OXYGEN SPEACIES IN ELICITATION OF DEFENSE RESPONSES IN PEA PLANTS

INATA Atsuko, FUJII Masahiro, KIBA Akinori, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology   38   s92   1997年3月

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EFFECTS OF SYNTHETIC GLYCOPEPTIDE-ELICITORS ON DEFENSE RESPONSES OF PEA PLANTS

KIBA Akinori, INATA Atsuko, TAKEDA Tadahiro, KANEMITSU Takuya, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology   38   s93   1997年3月

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PLANT CELL WALL - THE PRIMARY APPARATUS RECOGNIZING AND RESPONDING TO FUNGAL SIGNALS

SHIRAISHI Tomonori, YAMADA Tetsuji, ICHINOSE Yuki, KIBA Akinori

Plant and cell physiology   38   s11   1997年3月

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記述言語：英語  

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Combined effects of multiple cis-acting elements in elicitor-mediated activation of PSCHS1 gene

H Seki, Y Ichinose, M Ito, T Shiraishi, T Yamada

PLANT AND CELL PHYSIOLOGY   38 ( 1 )   96 - 100   1997年1月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

Promoter of a gene encoding chalcone synthase 1 (PSCHS1), a member of the defense-related genes in pea, was analyzed by transient transfection assay. The results demonstrated that in addition to the previously identified AT-rich sequences, at least four distinct cis-acting elements were required for maximal fungal elicitor-mediated activation of PSCHS1.

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バナジン酸によるエンドウ培養細胞におけるフィトアレキシン生産の誘導(英文)

河原 智治, 豊田 和弘, 木場 章範, 一瀬 勇規, 山田 哲治, 白石 友紀

岡山大学農学部学術報告   86   33 - 41   1997年

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記述言語：英語   出版者・発行元：岡山大学  

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Association between ion fluxes and defense responses in pea and cowpea tissues(共著)

Plant Cell Physiology   38 ( 6 )   698 - 706   1997年

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DOI： 10.1093/oxfordjournals.pcp.a029223

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Temporal and spatial pattern of expression of the pea phenylalanine ammoria-lyase gene 1 promoter in transgenic tobacco(共著)

Plant Cell Physiology   38 ( 7 )   792 - 803   1997年

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The Effects of Expression of Procaryotic-type iaa Genes inAgrobacterium tumefaciens on Plant Tumorigenicity.

村上 泰弘, 西野 友規, 平嗣 良, YUNUS Ahmad, 一瀬 勇規, 白石 友紀, 山田 哲治

日本植物病理学会報   63 ( 5 )   377 - 380   1997年

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記述言語：英語   出版者・発行元：The Phytopathological Society of Japan  

植物関連細菌が持つ原核型iaa遺伝子の役割を調べるために,オリーブこぶ病菌(Pseudomonas syringae pv. savastanoi)のiaaオペロンを広宿主範囲ベクターpRK415に導入した組み換え体プラスミドpTOY1を構築し,これを根頭癌腫病菌(Agrobacterium tumefaciens A208)に導入した。A. t. A208は感染した植物細胞でのみIAAを生産するのに対し,組み換え体A. t. A208 (pTOY1)はそれとともに恒常的にIAAを生産する.組み換え体A. t. A208(pTOY1)を接種したトマト茎にはA. t. A208に比べて大きなクラウンゴールが形成され,さらにポテトディスクでもカルス誘導が促進された。一方,A. t. A208あるいはA. t. A208 (pTOY1)をポテトディスクに接種した後,植物防御応答に関連するphenylalanine ammonia-lyaseの酵素活性を経時的に調べると,A. t. A208よりもA. t. A208 (pTOY1)接種でポテトのPAL活性が低下した。したがって, A. t. A208 (pTOY1)の強病原性は, A. t. A208 (pTOY1)によって生産されたIAAによって感染現場で植物防御応答が抑制された結果であると考えられた。

DOI： 10.3186/jjphytopath.63.377

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The Effects of Expression of Procaryotic-type iaa Genes in Agrobacterium tumefaciems on Plant Tumorigenicity.(共著)

村上 泰弘, 西野 友規, 平嗣 良, 一瀬 勇規, 白石 友紀, 山田 哲治

Annals of the Phytopathological Society of Japan   63 ( 5 )   377 - 380   1997年

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記述言語：英語   出版者・発行元：The Phytopathological Society of Japan  

植物関連細菌が持つ原核型iaa遺伝子の役割を調べるために,オリーブこぶ病菌(Pseudomonas syringae pv. savastanoi)のiaaオペロンを広宿主範囲ベクターpRK415に導入した組み換え体プラスミドpTOY1を構築し,これを根頭癌腫病菌(Agrobacterium tumefaciens A208)に導入した。A. t. A208は感染した植物細胞でのみIAAを生産するのに対し,組み換え体A. t. A208 (pTOY1)はそれとともに恒常的にIAAを生産する.組み換え体A. t. A208(pTOY1)を接種したトマト茎にはA. t. A208に比べて大きなクラウンゴールが形成され,さらにポテトディスクでもカルス誘導が促進された。一方,A. t. A208あるいはA. t. A208 (pTOY1)をポテトディスクに接種した後,植物防御応答に関連するphenylalanine ammonia-lyaseの酵素活性を経時的に調べると,A. t. A208よりもA. t. A208 (pTOY1)接種でポテトのPAL活性が低下した。したがって, A. t. A208 (pTOY1)の強病原性は, A. t. A208 (pTOY1)によって生産されたIAAによって感染現場で植物防御応答が抑制された結果であると考えられた。

DOI： 10.3186/jjphytopath.63.377

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Orthovanadate induces phytoalexiu production in pea suspension-cultured cells(共著)

河原 智治, 豊田 和弘, 木場 章範, 一瀬 勇規, 山田 哲治, 白石 友紀

Scientific Reprots of the Fawlty of Agriculture OKAYAMA UNIVERSITY   86   33 - 41   1997年

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記述言語：英語   出版者・発行元：岡山大学  

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dad-1, a putative programmed cell death suppressor gene in rice(共著)

Plant Cell Physiology   38 ( 3 )   379 - 383   1997年

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Mechanisms of the plant disease resistance-Focusing on the suppressor-(共著)

32 ( 1 )   49 - 59   1997年

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DOI： 10.18978/jscrpi.32.1_49

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植物の病害抵抗性機構-特にサプレッサーについて-

植物の化学調節   32 ( 1 )   49 - 59   1997年

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DOI： 10.18978/jscrpi.32.1_49

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Mechanisms of the plant disease resistance-Focusing on the suppressor-(共著)

32 ( 1 )   49 - 59   1997年

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DOI： 10.18978/jscrpi.32.1_49

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The role of suppressors in determining host-parasite specificities in plant cells

T Shiraishi, T Yamada, Y Ichinose, A Kiba, K Toyoda

INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 172   172   55 - 93   1997年

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記述言語：英語   掲載種別：書評論文，書評，文献紹介等   出版者・発行元：ELSEVIER ACADEMIC PRESS INC  

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Analysis of cis-regulatory elements involved in the activation of a member of chalcone synthase gene family (PsChs1) in pea

H Seki, Y Ichinose, H Kato, T Shiraishi, T Yamada

PLANT MOLECULAR BIOLOGY   31 ( 3 )   479 - 491   1996年6月

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記述言語：英語   出版者・発行元：KLUWER ACADEMIC PUBL  

Cis-regulatory elements involved in the activation of the plant defense-related gene encoding chalcone synthase 1 (PsChs1) in pea (Pisum sativum L.) were examined by transient transfection, gel mobility shift assay and in vitro DNase I-footprinting analysis. Transient transfection assay revealed that a 61 bp DNA fragment spanning from -242 to -182 of PsChs1 was required for the maximal promoter activity and possibly involved in the enhancement of elicitor-mediated activation. Nuclear isolate from elicitor-treated pea epicotyl tissues contained some factor(s) that specifically bound to this DNA fragment to form a complex with low mobility (LMC, low mobility complex) in gel mobility shift assay. DNase I-footprinting analysis of LMC revealed that among three protected regions detected in a 61 bp DNA fragment, two regions contained identical AT-rich sequence, TAAAATACT. Site directed mutation in either or both identical sequences, T&lt;(AA)under bar&gt;AATACT to T&lt;(GG)under bar&gt;AATACT, resulted in the reduction or loss in the ability to form LMC. Detailed analysis of 61 bp DNA fragment demonstrated that the region from -242 to -226 containing promoter-distal TAAAATACT motif was imperative for the maximal elicitor-mediated activation of PsChs1.

DOI： 10.1007/BF00042222

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Specific response of partially purified cell wall-bound ATPases to fungal suppressor(共著)

Plant Cell Physiology   37 ( 2 )   207 - 214   1996年3月

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記述言語：英語  

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REGULATION OF THE GENE ENCODING PHENYLALANINE AMMONIA-LYASE OF PEA IN TRANSGENIC TOBACCO

IMURA Yoshiyuki, MORIKAWA Akiko, MICHAELZACK Dalius, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology   37   54 - 54   1996年3月

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記述言語：英語  

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MOLECULAR EVOLUTION OF DEFENSE RELATED CHALCONE SYNTHASE GENES IN PEA

ITOH Masayuki, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology   37   54 - 54   1996年3月

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記述言語：英語  

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Suppression of ATPase activity in pea cells during infecfion by a compatible race of Pseudomouas syriugae pv. pisi(共著)

下間 奈津美, 林 昌治, MALIK Kamal A., 一瀬 勇規, 白石 友紀, 山田 哲治

Annals of Phytopathological Society of Japan   62 ( 5 )   498 - 501   1996年

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記述言語：英語   出版者・発行元：日本植物病理学会  

エンドウ褐紋病菌が胞子発芽液中に分泌する低分子糖ペプチド (supprescin) は, 宿主エンドウの原形質膜ATPase活性を阻害することにより初期の防御応答を抑制している。植物病原細菌が病原糸状菌のsupprescinと類似の機能を有する生体分子を保有するか検討を加えた。暗黒下で生育させた幼エンドウ植物体の上胚軸あるいは成熟葉にエンドウつる枯れ病細菌を接種し, in vivoにおける原形質膜ATPase活性をリン酸鉛の沈着によって組織化学的に解析した結果, 親和性関係においてのみ原形質膜ATPaseの活性が一時的に阻害された。レース・品種の異なる組み合わせにおいても原形質膜ATPaseの阻害は親和性レースを接種した組織においてのみ観察され, 非親和性レースの接種では観察されなかった。植物病原糸状菌のsupprescinに類似の機能を有する植物病原細菌のサプレッサー分子の存在の可能性, さらに病原細菌の病原性における作用機作について論ずる。

DOI： 10.3186/jjphytopath.62.498

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Species-specific suppression of superoxide-anion generation of surfaces of pea leaves by the suppressor from Mycosphaerella pinodes(共著)

Annals of the Phytopathological Society of JAPAN   62 ( 5 )   508 - 512   1996年

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DOI： 10.3186/jjphytopath.62.508

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Species-specific suppression of superoxide-anion generation of surfaces of pea leaves by the suppressor from Mycosphaerella pinodes(共著)

KIBA A, TOYODA K, ICHINOSE Y, YAMADA T, SHIRAISHI T

Annals of the Phytopathological Society of JAPAN   62 ( 5 )   508 - 512   1996年

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記述言語：英語   出版者・発行元：日本植物病理学会  

DOI： 10.3186/jjphytopath.62.508

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Analysis of cis-regulatory elements involued in the activation of a member of chalcoue synthase gene family (PsCHS1) in pea(共著)

Plant Molecular Biology   1996年

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DOI： 10.1007/BF00042222

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エンドウつる枯れ病細菌の感染によるエンドウ原形質膜ATPaseのin vivoにおける阻害

下間 奈津美, 林 昌治, MALIK Kamal A., 一瀬 勇規, 白石 友紀, 山田 哲治

日本植物病理學會報   62 ( 5 )   498 - 501   1996年

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記述言語：英語   出版者・発行元：日本植物病理学会  

エンドウ褐紋病菌が胞子発芽液中に分泌する低分子糖ペプチド (supprescin) は, 宿主エンドウの原形質膜ATPase活性を阻害することにより初期の防御応答を抑制している。植物病原細菌が病原糸状菌のsupprescinと類似の機能を有する生体分子を保有するか検討を加えた。暗黒下で生育させた幼エンドウ植物体の上胚軸あるいは成熟葉にエンドウつる枯れ病細菌を接種し, in vivoにおける原形質膜ATPase活性をリン酸鉛の沈着によって組織化学的に解析した結果, 親和性関係においてのみ原形質膜ATPaseの活性が一時的に阻害された。レース・品種の異なる組み合わせにおいても原形質膜ATPaseの阻害は親和性レースを接種した組織においてのみ観察され, 非親和性レースの接種では観察されなかった。植物病原糸状菌のsupprescinに類似の機能を有する植物病原細菌のサプレッサー分子の存在の可能性, さらに病原細菌の病原性における作用機作について論ずる。

DOI： 10.3186/jjphytopath.62.498

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EXPRESSION OF HETEROLOGOUS PAL GENE IN MUSKMELON (CUCUMIS-MELO L CV BIRDIE)

NM SALEH, SH TAN, Y ICHINOSE, T YAMADA

ASIA-PACIFIC JOURNAL OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   3 ( 3 )   206 - 214   1995年9月

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記述言語：英語   出版者・発行元：MALAYSIAN SOC MOLECULAR BIOLOGY BIOTECHNOLOGY  

A system to develop PAL gene expression using particle gun was established in Cucumis melo L. These techniques enable us to study the regulation of pea phenylalanine ammonia-lyase (PAL) promoters in a heterologous plant system. The effect of UV irradiation on the regulation of the PAL promoters under different regime of UV irradiation were tested on the mesophyll protoplasts of melon. Maximum induction of GUS expression were observed in muskmelon leaves electroporated with two members of pea PAL promoters, PSPAL1 and PSPAL2 at 120 sec and 80 sec, respectively. Further confirmation of the GUS expression in the tissue of muskmelon were carried out using particle bombardment. Similar pattern of induction of GUS expression by UV irradiation have been observed, which suggests that common mechanism of PAL gene expression may exist in both muskmelon plant.

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Expression of heterologous pal gene in Muskmelon(Cucumis melo L. Birdie)(共著)

Asia pacific Journal of Molecular Biology and Biotechndogy   3 ( 3 )   206 - 214   1995年9月

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記述言語：英語  

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PLANT-LECTINS INDUCE THE PRODUCTION OF A PHYTOALEXIN IN PISUM-SATIVUM

K TOYODA, K MIKI, Y ICHINOSE, T YAMADA, T SHIRAISHI

PLANT AND CELL PHYSIOLOGY   36 ( 5 )   799 - 807   1995年7月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

The effects of several plant lectins on the production of a pea phytoalexin, pisatin, were examined. Con A, PHA, PNA and PSA each induced the production of pisatin in pea epicotyl tissues, demonstrating that plant lectins can act as elicitors. The production of pisatin in response to PHA, PNA or PSA was not affected by the simultaneous presence of the respective hapten sugars, whereas haptens specific for Con A, such as alpha-D-mannose and methyl-alpha-D-mannoside, abolished the induction of pisatin by Con A. These results indicate that the elicitor effect of Con A is attributable to its ability to bind to specific carbohydrates in pea cells. Induction of the production of pisatin by Con A was markedly inhibited by the suppressor derived from a pea pathogen. Mycosphaerella pinodes, and by several inhibitors related to signal-transduction pathways. It is suggested, therefore, that the Con A-induced production of pisatin in pea tissues might be associated with activation of a signal-transduction pathway. An additive effect on the accumulation of pisatin was observed when Con A was present with a polysaccharide elicitor from M. pinodes, suggesting that exogenous Con A does not compete with the recognition site(s) for the fungal elicitor in pea cells. The present data also indicate that Con A may be useful for characterization of the signal-transduction system that leads to the synthesis of phytoalexin in pea epicotyl tissues.

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SPECIFIC-INHIBITION OF CELL WALL-BOUND ATPASES BY FUNGAL SUPPRESSOR FROM MYCOSPHAERELLA-PINODES

A KIBA, K TOYODA, Y ICHINOSE, T YAMADA, T SHIRAISHI

PLANT AND CELL PHYSIOLOGY   36 ( 5 )   809 - 817   1995年7月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

Activities of phosphatases were found in the fractions which were solubilized from cell walls of both pea and cowpea seedlings with 0.5 M NaCl. These phosphatases hydrolyzed triphosphonucleotides in the order: UTP = CTP &gt; GTP &gt; ATP; and UTP = GTP &gt; CTP = ATP, respectively. The activities of a pyrophosphatase and a p-nitrophenylphosphatase were also detected in these fractions. The suppressor in the spore germination fluid of a pea pathogen, Mycosphaerella pinodes, inhibited all of these phosphatase activities in the fraction solubilized from pea cell walls, but it rather enhanced only the activity of the ATPase among those phosphatases from the cowpea cell wall. Hydrolysis of ATP by a cell wall fraction of pea was also markedly inhibited by the suppressor, while hydrolysis of ATP by similar fractions from cowpea, kidney bean and soybean were rather enhanced by the suppressor, as well as by the elicitor. Thus, the cell wall-bound ATPases responded to the suppressor species-specifically. These cell wall-bound ATPases seemed to be different from the plasma membrane ATPases in several respects. The results suggest that plants recognize the fungal signals not only on their plasma membranes but also on their cell walls and, moreover that putative receptors for the fungal signals might be located close to cell wall-bound ATPases or might even be these ATPases themselves.

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Plant lectins induce the production of a phytolexin in Pisum sativum(共著)

Plant Cell Physiology   36 ( 5 )   799 - 807   1995年7月

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記述言語：英語  

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Specific inhibition of cell wall-bound ATPase by fungal suppressor from Mycosphaerella pinodes(共著)

Plant Cell Physiology   36 ( 5 )   809 - 817   1995年7月

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記述言語：英語  

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Establishment of a system for the transient expression of GUS gene in electroporated muskmelon (Cucumis melo L) protoplasts. (共著)

Asia Pacific Journal of Molecular Biology and Biotechnology   3 ( 2 )   156 - 160   1995年6月

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記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

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A SUPPRESCIN FROM A PHYTOPATHOGENIC FUNGUS DEACTIVATES TRANSCRIPTION OF A PLANT DEFENSE GENE ENCODING PHENYLALANINE AMMONIA-LYASE

M WADA, H KATO, K MALIK, P SRIPRASERTSAK, Y ICHINOSE, T SHIRAISHI, T YAMADA

JOURNAL OF MOLECULAR BIOLOGY   249 ( 3 )   513 - 519   1995年6月

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記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：ACADEMIC PRESS (LONDON) LTD  

Both elicitor and supprescin (suppressor) are present in the pycnospore germination fluid of a pea pathogen Mycospharella pinodes. A nuclear run-on assay revealed that supprescin rapidly deactivated elicitor-triggered transcription of the gene encoding phenylalanine ammonia-lyase in pea epicotyl tissues. The mechanism underlying the deactivation of the plant defense gene by signal molecules secreted from the fungal pathogen was investigated. Cis-acting sequences and trans-acting factors responsive to supprescin in a TATA-proximal region of a member of the phenylalanine ammonia-lyase gene family in pea were examined in vitro. Gel mobility-shift assays and DNase I footprinting analysis revealed that the promoter region of PSPAL2 was modified by the binding of nuclear factors at multiple sites that were possibly involved in supprescin-mediated deactivation. The prominent changes by supprescin were observed at boxes 2 and 4 and near exonic sequences.

DOI： 10.1006/jmbi.1995.0313

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CHARACTERIZATION OF NUCLEAR FACTORS FOR ELICITOR-MEDIATED ACTIVATION OF THE PROMOTER OF THE PEA PHENYLALANINE AMMONIA-LYASE GENE

H KATO, M WADA, K MURAYA, K MALIK, T SHIRAISHI, Y ICHINOSE, T YAMADA

PLANT PHYSIOLOGY   108 ( 1 )   129 - 139   1995年5月

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記述言語：英語   出版者・発行元：AMER SOC PLANT PHYSIOLOGISTS  

The nuclear factors presumably associated with the activation of the gene encoding phenylalanine ammonia-lyase by a fungal elicitor were characterized in pea (Pisum sativum L.) epicotyls. The TATA-proximal region was dissected and putative cis-regulatory elements in the promoter of pea phenylalanine ammonia-lyase gene 1 were examined by gel-mobility shift and in vitro footprinting analyses. Specific binding of the nuclear factors to the promoter-proximal regions of pea phenylalanine ammonia-lyase gene 1 associated with elicitor-mediated activation was detected at a region containing consensus sequence motifs of boxes 2 and 4 and other AT-rich sequences. The analyses of DNA fragments containing the deleted promoter regions suggested that a residue from -183 to -173 (ATTAGTAAGTGAT) was essential for a maximal activity of forming low-mobility complex (LMC) in the gel-mobility shift assay, and synthetic oligonucleotides confirmed the presence of at least one nuclear component associated with the formation of an active LMC. Competition experiments and treatment with Hoechst 33258 provided direct evidence that the formation of LMC with the promoter fragments from genes encoding phenylalanine ammonia-lyase and chalcone synthase in pea contained one or more of the same proteins that recognize AT-rich sequence motifs for binding. It also suggests that common high-mobility group-like proteins might be involved in the regulation of elicitor-inducible genes in pea.

DOI： 10.1104/pp.108.1.129

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RELATIONSHIP BETWEEN DEFENSE RESPONSESAND ION FLUX

AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology   36   S119   1995年3月

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記述言語：英語  

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TRANSCRIPTIONAL REGULATION OF THE GENEENCODING CHALCONE SYNTHASE IN PEA BY FUNGAL ELICITOR

SEKI Hikaru, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology   36   S120   1995年3月

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記述言語：英語  

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REGULATION OF PLANT DEFENSE RESPONSES BY SIGNALS FROM FUNGAL PATHOGENS

T SHIRAISHI, T YAMADA, Y ICHINOSE

NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY   69 ( 2 )   174 - 177   1995年2月

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記述言語：日本語   掲載種別：書評論文，書評，文献紹介等   出版者・発行元：JAPAN SOC BIOSCI BIOTECHN AGROCHEM  

DOI： 10.1271/nogeikagaku1924.69.174

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THE RELATIONSHIP BETWEEN SYSTEMIC RESISTANCE INDUCED BY PRUNING AND ACCUMULATION OF ANTIFUNGAL SUBSTANCES IN BARLEY SEEDLINGS

T HIRAMOTO, N ABE, R TOBIMATSU, T SHIRAISHI, H OKU, T YAMADA, Y ICHINOSE

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT   143 ( 1 )   43 - 46   1995年1月

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記述言語：英語   出版者・発行元：BLACKWELL WISSENSCHAFTS VERLAG GMBH  

Infection by a compatible race of Erysiphe graminis f. sp. hordei on barley secondary leaves was significantly suppressed upon pruning of the primary leaves when E. graminis hordei was inoculated 3-12 h after the pruning, but it was rather enhanced during 15-21 h. The accumulation of antifungal substances was detected in hot ethanol extracts of barley seedlings from 15-27 h after pruning the primary leaves. Taking the time of the infection process of a challenger (E. graminis hordei) into consideration, timing of systemic resistance induced upon pruning coincided with the accumulation of antifungal substances.

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SIGNAL MOLECULES INDUCING SYSTEMIC RESISTANCE AND SUSCEPTIBILITY IN BARLEY SEEDLINGS

T HIRAMOTO, R TOBIMATSU, N ABE, T SHIRAISHI, H OKU, T YAMADA, Y ICHINOSE

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT   143 ( 1 )   47 - 51   1995年1月

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記述言語：英語   出版者・発行元：BLACKWELL WISSENSCHAFTS VERLAG GMBH  

Exudate collected from the cut end of barley seedlings exhibited both activities that induced systemic resistance and susceptibility against Erysiphe graminis f. sp. hordei race Hh4 depending on the time after pruning. Exudates collected between 3-6 h after pruning showed maximum activity that induced systemic resistance, whereas those during 9-12 h conversely induced susceptibility in barley seedlings. The accumulation of antifungal substances in barley leaves correlates to the timing of induced resistance. The antifuntingal substances were water-soluble and severely affected the infection of E. graminis f. sp. hordei.

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Characterization of nuclear factors for elicitor-mediated activation of the promoter of the pea phenylalanine ammonia-lyase gene 1. (共著)

KATO H, WADA M, MURAYA K, MALIK K, SHIRAISHI T, ICHINOSE Y, YAMADA T

Plant Physiology   108 ( 1 )   129 - 139   1995年

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DOI： 10.1104/pp.108.1.129

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Suppression of the activation of chitinase and β-1, 3-glucanase in pea epicotyls by endogenous suppressor from ┣DBPisum sativum(/)-┫DB. (共著)

Annals of the Phytopathological Society of Japan   1995年

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Regulation of plant defense responses by signals from fangal pathogeus

Tomonori Shiraishi, Tetsuji Yamada, Yuki Ichinose

Nippon N(]E87C7[)geikagaku Kaishi   69 ( 2 )   174 - 177   1995年

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DOI： 10.1271/nogeikagaku1924.69.174

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病原菌シグナルと植物の防御応答 共著

白石 友紀, 山田 哲治, 一瀬 勇規

日本農芸化学会誌   69 ( 2 )   174 - 177   1995年

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DOI： 10.1271/nogeikagaku1924.69.174

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H⁺ - translocating activity in proteoliposomes reconstituted with pea plasma membrane ATPase and its inhibition by fungal suppnessor from Mycosphaerella pinodes(共著)

Annals of Phytopathological Society of Japan   61   134 - 136   1995年

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Supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encodig phenylalanine ammonia-lyase. (共著)

Journal of Molecular Biology   1995年

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DOI： 10.1006/jmbi.1995.0313

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Signal molecules inducing systemic resistance and susceptibility in barley seedling. (共著)

Journal of Phytopathology   143   1995年

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The relationship between systemic resistance induced by pruning and accumulation of anitifungal substances in barley seedling. (共著) 査読

Journal of Phytopathology   143   47 - 51   1995年

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担当区分：最終著者  

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Suppression of the activation of chitinase and β-1, 3-glucanase in pea epicotyls by endogenous suppressor from ┣DBPisum sativum(/)-┫DB. (共著)

那須 公雄, 吉岡 博文, 山田 哲治, ー瀬 勇規, 奥 八郎, 白石 友紀

Annals of the Phytopathological Society of Japan   61 ( 1 )   13 - 17   1995年

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記述言語：英語   出版者・発行元：日本植物病理学会  

健全エンドウ苗中に存在する内生サプレッサーは, エンドウ褐紋病菌のエリシターで誘導されるピサチン蓄積を抑制し, 本サプレッサーで処理されたエンドウには非病原菌が感染できるようになる。本報告では, 抵抗反応の一つと考えられるキチナーゼとβ-1,3-グルカナーゼの活性化に及ぼす内生サプレッサーの影響について調べた。エンドウ, インゲン, ダイズの肛軸組織をエリシターで処理すると, 両酵素の活性化が誘導された。しかし, 内生サプレッサーの共存下には, 供試植物中エンドウでのみ活性増高が抑制された。ー方, インゲンおよびダイズでは, 内生サプレッサーの単独処理においても, 両酵素の活性増高が誘導された。これらの結果は, 植物防御応答に対する内生サプレッサーの作用が種特異的であり, 作用様式において褐紋病菌サプレッサーと酷似することを示している。

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その他リンク： http://agriknowledge.affrc.go.jp/RN/2010520740

H⁺ - translocating activity in proteoliposomes reconstituted with pea plasma membrane ATPase and its inhibition by fungal suppnessor from Mycosphaerella pinodes(共著)

Annals of Phytopathological Society of Japan   61   134 - 136   1995年

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Supperssors : Determinants of specificity produced by plant pathogens. (共著)

Plant Cell Phisiology   35 ( 8 )   1107 - 1119   1994年12月

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記述言語：英語   掲載種別：書評論文，書評，文献紹介等  

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Suppressor produced by a pea pathogen, ┣DBM(/)-┫DB. pinodes. (共著)

Grain Legumes   4   22 - 23   1994年

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Suppressor produced by a pea pathogen, ┣DBM(/)-┫DB. pinodes. (共著)

Grain Legumes   4   22 - 23   1994年

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Rapid Changes in Polyphosphoinositide Metabolism in Pea in Response to Fungal Signals 共著

Plant Cell Physiology   34 ( 5 )   729 - 735   1993年7月

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記述言語：英語  

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ORGANIZATION OF THE GENES ENCODING CHALCONE SYNTHASE IN PISUM-SATIVUM

C AN, Y ICHINOSE, T YAMADA, Y TANAKA, T SHIRAISHI, H OKU

PLANT MOLECULAR BIOLOGY   21 ( 5 )   789 - 803   1993年3月

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記述言語：英語   出版者・発行元：KLUWER ACADEMIC PUBL  

To analyze the regulation of defense-related genes by signal molecules produced by phytopathogens, we isolated genes that encode chalcone synthase (CHS) in Pisum sativum. We have obtained seven independent genomic clones that contain at least seven classes of CHS genes, identified by the hybridization analysis to CHS cDNA and by the restriction mapping analysis. Two of the genomic clones (clone 5 and 6) each contain two CHS genes in a tandem repeat. The nucleotide sequence analysis of CHS genomic clone 5 revealed that PsCHS1 and PsCHS2 were corresponding genes of the CHS cDNA clones, pCC6 and pCC2, respectively, as reported earlier. Both genes are interrupted by a single intron of 88 nucleotides with identical sequences, although exonic sequences and 5'-flanking sequences are divergent. Nucleotide sequences of the introns in five other classes of CHS genes showed that three classes had an intron of 87 nt with a striking homology to each other, but that the intron of the other two classes of CHS genes showed heterogeneity both in size and nucleotide sequence. 5'-upstream regions of PsCHS1 and PsCHS2 did not show sequence homology except the 31 bp identical sequence that contains the CCTACC motif resembling the box-1 sequence. Both PsCHS1 and PsCHS2 genes are shown to be induced by fungal elicitor by a primer extension analysis and a transient transformation analysis using pea protoplasts prepared from suspension cultured-cells.

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Organization of the genes encoding chalcone synthase in Pisum sativum 共著

Plant Molecular Biology   21 ( 5 )   789 - 803   1993年3月

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記述言語：英語  

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MOLECULAR-CLONING OF PHENYLALANINE AMMONIA-LYASE CDNA FROM PISUM-SATIVUM

S KAWAMATA, T YAMADA, Y TANAKA, P SRIPRASERTSAK, H KATO, Y ICHINOSE, H KATO, T SHIRAISHI, H OKU

PLANT MOLECULAR BIOLOGY   20 ( 1 )   167 - 170   1992年10月

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記述言語：英語   出版者・発行元：KLUWER ACADEMIC PUBL  

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Phenylalanine ammonia-lyase genes from Pisum sativum : Structure, organ-specific expression and regulation by fungal elicitor and suppressor(共著)

Plant Cell Physiology   33 ( 6 )   715 - 725   1992年9月

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記述言語：英語  

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AN ENDOGENOUS SUPPRESSOR OF THE DEFENSE RESPONSE IN PISUM-SATIVUM

K NASU, T SHIRAISHI, H YOSHIOKA, N HORI, Y ICHINOSE, T YAMADA, H OKU

PLANT AND CELL PHYSIOLOGY   33 ( 5 )   617 - 626   1992年7月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

Healthy pea plants contain a substance, tentatively called "endogenous suppressor", which specifically suppresses the accumulation of pisatin in pea plants that is induced by treatment with CuCl2 or an elicitor from Mycosphaerella pinodes. This suppressor elicits the accumulation of phytoalexins in other legumes, such as kidney bean, soybean and cowpea. The endogenous suppressor functions to delay the accumulation of pisatin, the activation of phenylalanine ammonialyase (PAL) and the accumulation of mRNAs for PAL and chalcone synthase induced by the elicitor from M. pinodes. The substance specifically induces susceptibility to nonpathogens, such as Mycosphaerella ligulicola and M. melonis, in pea out of four species of legume tested, but the effect is not cultivar-specific. Thus, the endogenous suppressor in healthy pea plants suppresses a series of self-defense reactions and induces susceptibility in pea plants in a species-specific manner, being similar to the exogenous fungal suppressor from the pea pathogen, M. pinodes.

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2-SUPPRESSORS, SUPPRESCINS-A AND SUPPRESCINS-B, SECRETED BY A PEA PATHOGEN, MYCOSPHAERELLA-PINODES

T SHIRAISHI, K SAITOH, HM KIM, T KATO, M TAHARA, H OKU, T YAMADA, Y ICHINOSE

PLANT AND CELL PHYSIOLOGY   33 ( 5 )   663 - 667   1992年7月

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記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

Two mucin-type glycopeptides that suppressed the production of pisatin, a phytoalexin of pea, were purified from a pea pathogen, Mycosphaerella pinodes. The structures of Supprescin A (Mr, 452) and Supprescin B (Mr, 959) were determined by an analysis of amino acid sequences and C-13- and H-1-NMR.

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Regulation of phosphoinositide metabolism in pea plasma membranes by elicitor and suppressor from a pea pathogen, Mycosphaerella pinodes(共著)

Plant Cell Physiology   33 ( 4 )   445 - 452   1992年6月

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記述言語：英語  

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ENDOGENOUS ELICITOR PRESENT IN BARLEY-SEEDS .2. MECHANISMS OF THE INDUCTION OF THE RESISTANCE IN BARLEY LEAVES TO ERYSIPHE-GRAMINIS

T HIRAMOTO, R TOBIMATSU, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT   135 ( 2 )   167 - 176   1992年6月

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記述言語：英語   出版者・発行元：BLACKWELL WISSENSCHAFTS-VERLAG GMBH  

Endogenous elicitor(s) present in barley seeds induce the accumulation of antifungal substances effective against powdery mildew fungi. Antifungal substances induced by the treatment with barley seeds extract coincides with the one induced by the inoculation with compatible or incompatible races of powdery mildew fungi on thin-layer chromatography analysis. The resistance induced by the treatment with barley seeds extract correlates to the accumulation of the antifungal substances in barley leaves. Furthermore, the activity of phenylalanine ammonia-lyase was also induced by the treatment with barley seeds extract.

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TRANSIENT EXPRESSION IN ELECTROPORATED PEA PROTOPLASTS - ELICITOR RESPONSIVENESS OF A PHENYLALANINE AMMONIA-LYASE PROMOTER

T HASHIMOTO, T YAMADA, A TADA, S KAWAMATA, Y TANAKA, P SRIPRASERTSAK, Y ICHINOSE, H KATO, S IZUTSU, T SHIRAISHI, H OKU, Y OHTSUKI

PLANT CELL REPORTS   11 ( 4 )   183 - 187   1992年5月

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記述言語：英語   出版者・発行元：SPRINGER VERLAG  

High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (&gt; 100-mu-g/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.

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MOLECULAR-CLONING OF CHALCONE SYNTHASE CDNAS FROM PISUM-SATIVUM

Y ICHINOSE, S KAWAMATA, T YAMADA, CC AN, T KAJIWARA, T SHIRAISHI, H OKU

PLANT MOLECULAR BIOLOGY   18 ( 5 )   1009 - 1012   1992年3月

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記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：KLUWER ACADEMIC PUBL  

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Orthovanadate suppresses on accumulation of phenylalanine ammonia-lyase mRNA and chalcone synthase mRNA in pea epicotyls that is induced by an elicitor from Mycosphaerella pinodes(共著)

Plant Cell Physiology   33 ( 2 )   201 - 204   1992年3月

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記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

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Molecular cloning of phenylalanine ammonia-lyase cDNA from Pisum sativum 共著

Plant Molecular Biology   20,167-170   1992年

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Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by orthovanadate and a suppressor from Mycosphae-rella pinodes 共著

Annals of the Phytopathological Society of JAPAN   58,405-410   1992年

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DOI： 10.3186/jjphytopath.58.405

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Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by orthovanadate and a suppressor from Mycosphae-rella pinodes 共著

吉岡 博文, 白石 友紀, 那須 公雄, 山田 哲治, 一瀬 勇規, 奥 八郎

Annals of the Phytopathological Society of JAPAN   58,405-410 ( 3 )   405 - 410   1992年

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記述言語：英語   出版者・発行元：日本植物病理学会  

エンドウ褐紋病菌 Mycosphaerella pinodes はその柄胞子発芽液中にエリシターおよびサプレッサーを分泌する。本報告では,抵抗反応の一つと考えられているキチナーゼおよびβ-1,3-グルカナーゼの活性化に及ぼすエリシターとサプレッサーおよびP型ATPaseの阻害剤であるバナジン酸の影響を調べた。エンドウ, ダイズ, インゲンをエリシターで処理すると, 両酵素の活性増高が誘導された。しかし, 1mMバナジン酸の共存下では, いずれの植物においても活性の増高は抑制された。一方, サプレッサーは供試植物の中でエンドウに対してのみ抑制効果を示した。本結果は, 原型質膜ATPaseの阻害とキチナーゼおよびβ-1,3-グルカナーゼの抑制に強い関連のあることを示唆している。

DOI： 10.3186/jjphytopath.58.405

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An endogenous suppressor of the defense response in Pisum sativum 共著

Plant Cell Physiology   33,617-626   1992年

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Endogenous elicitor present in barley seeds II. Mechanisms of the induction of the resistance in barley leaves to Erysiphe graminis 共著

Journal of Phytopathology   135,167-176   1992年

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A reporter gene expression under the control of a pea phenylalanine ammonia-lyase gene promoter(共著)

山田 哲治, 橋本 忠明, スリプラサートサック ペルンポン[他]

Scientific Reports of the Faculty of Agriculture Okayama University   80,51-60   51 - 60   1992年

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記述言語：英語   出版者・発行元：岡山大学  

High yields of viable pea protoplasts were produced from suspension cultured cells derived from calli formed from embryogenic tissues or leaves and the conditions for the optimum expression of chloramphenicol acetyltransferase (CAT) fused to the phenylalanine ammonia-lyase gene of Pisum sativum (pPAL1-15) were investigated by transient assay after electroporation. A fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and the reduced from of glutathione induced the expression of PAL promoter but orthovanadate, a plasma membrane ATPase inhibitor, considerably suppressed the gene expression. Rice protoplasts were also prepared from the suspension cultured cells derived from embryonic tissues, and the effects of elicitors on the expression of CAT in pPAL1-15-electroporated rice protoplasts were examined. No distinctive induction of CAT activity was observed by the treatment of rice protoplasts with a chitosan oligomer elicitor.

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その他リンク： http://ousar.lib.okayama-u.ac.jp/849

TWO suppressors, suppresciws A and B secreted by a pea pathogen, Mycosphaerella pinodes 共著

Plant Cell Physiology   33,663-667   1992年

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TWO suppressors, suppresciws A and B secreted by a pea pathogen, Mycosphaerella pinodes 共著

Plant Cell Physiology   33,663-667   1992年

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Molecular cloning of chalcone synthase cDNA frow Pisum sativum 共著

Plant Molecular Biology   18,1009-1012   1992年

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エンドウプロトプラスト細胞における植物プロモータの発現(英文)

山田 哲治, 橋本 忠明, スリプラサートサック ペルンポン[他]

岡山大学農学部学術報告   80,51-60   51 - 60   1992年

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記述言語：英語   出版者・発行元：岡山大学  

High yields of viable pea protoplasts were produced from suspension cultured cells derived from calli formed from embryogenic tissues or leaves and the conditions for the optimum expression of chloramphenicol acetyltransferase (CAT) fused to the phenylalanine ammonia-lyase gene of Pisum sativum (pPAL1-15) were investigated by transient assay after electroporation. A fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and the reduced from of glutathione induced the expression of PAL promoter but orthovanadate, a plasma membrane ATPase inhibitor, considerably suppressed the gene expression. Rice protoplasts were also prepared from the suspension cultured cells derived from embryonic tissues, and the effects of elicitors on the expression of CAT in pPAL1-15-electroporated rice protoplasts were examined. No distinctive induction of CAT activity was observed by the treatment of rice protoplasts with a chitosan oligomer elicitor.

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Transient expression in electroporated pea protoplasts : Elicitor responsiveness of a phenylalanine ammonia-lyase promoter 共著 国際誌

Plant Cell Reports   11,183-187 ( 4 )   183 - 7   1992年

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記述言語：英語  

DOI： 10.1007/BF00232529

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Inhibition of ATPase in pea plasma membranes in situ by a suppressor from a pea pathogen, Mycospharella pinodes 共著

Plant Cell Physiology   32 ( 7 )   1067 - 1075   1991年10月

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記述言語：英語  

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エンドウ動的遺伝子の発現機構． 招待

山田哲治, 白石友紀, 一瀬勇規, 奥 八郎, 川又伸治, 田中良和, Spirasertsak, P, 安 成才, 吉岡博文, 橋本忠明

生物相互間の認識機構   27   56-65   1991年

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記述言語：日本語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

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SUPPRESSION OF PISATIN PRODUCTION AND ATPASE ACTIVITY IN PEA PLASMA-MEMBRANES BY ORTHOVANADATE, VERAPAMIL AND A SUPPRESSOR FROM MYCOSPHAERELLA-PINODES

H YOSHIOKA, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

PLANT AND CELL PHYSIOLOGY   31 ( 8 )   1139 - 1146   1990年12月

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記述言語：英語   出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS  

A pea pathogen, Mycosphaerella pinodes, secretes both an elicitor and a suppressor for the accumulation of pisatin, a major phytoalexin of pea, into the spore germination fluid. The effects of elicitor and the suppressor on the ATPase activity in pea plasma membranes was examined. The ATPase was sensitive to orthovanadate and dicyclohexylcarbodiimide but insensitive to nitrate and azide; it was unaffected by the elicitor but was markedly inhibited by the suppressor (50-mu-g.ml-1, bovine serum albumin equivalents) or verapamil (100-mu-M). The accumulation of pisatin induced by the elicitor was delayed for 3 to 6 h in the presence of orthovanadate or verapamil to an extent similar to that in the presence of the suppressor. The relationship between the inhibition of plasma membrane ATPase activity and the suppression of the active defense reaction that involves the production of pisatin in the pea plant is discussed.

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Identification of mouse endodermal cytoskeletal protein C (endo C) with cytokeratin no. 19 共著

Biochemical and Biophysical Research Communications   167 ( 2 )   644 - 647   1990年3月

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担当区分：筆頭著者   記述言語：英語  

DOI： 10.1016/0006-291X(90)92073-9

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Suppression of pisatin production and ATPase activity in pea plasma membranes by orthovanadate, verapamil and a suppressor from Mycosphaerella pinodes 共著

Plant Cell Physiology   31,1139-1146   1990年

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MOLECULAR-CLONING AND CHARACTERIZATION OF CDNA-ENCODING MOUSE CYTOKERATIN-NO-19

Y ICHINOSE, K HASHIDO, H MIYAMOTO, T NAGATA, M NOZAKI, T MORITA, A MATSUSHIRO

GENE   80 ( 2 )   315 - 323   1989年8月

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記述言語：英語   出版者・発行元：ELSEVIER SCIENCE BV  

DOI： 10.1016/0378-1119(89)90295-3

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Molecular cloning and characerization of cDNA encoding for mouse cytokeratin no. 19 共著

Gene   80,315-323   1989年

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DOI： 10.1016/0378-1119(89)90295-3

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NUCLEOTIDE-SEQUENCE AND STRUCTURE OF THE MOUSE CYTOKERATIN ENDOB GENE

Y ICHINOSE, T MORITA, FY ZHANG, S SRIMAHASONGCRAM, MLC TONDELLA, M MATSUMOTO, M NOZAKI, A MATSUSHIRO

GENE   70 ( 1 )   85 - 95   1988年10月

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記述言語：英語   出版者・発行元：ELSEVIER SCIENCE BV  

DOI： 10.1016/0378-1119(88)90107-2

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Nucleotide sequence of mouse endoA cytokeratin cDNA reveals a poly-peptide characterized to the type II keratin subfamily 共著

Gene   68 ( 1 )   109 - 117   1988年8月

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記述言語：英語  

DOI： 10.1016/0378-1119(88)90604-X

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Nucleotide sequence and structure of a mouse cytokeratin endoB gene 共著

Gene   70,85-95   1988年

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DOI： 10.1016/0378-1119(88)90107-2

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Regulation of pisatin biosynthesis in pea leaves by elicitor and suppressor produced by Mycosphaerella pinodes 共著

HIRAMATSU M.

Annals of the Phytopathological Society of JAPAN   52,53-58   53 - 58   1986年

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DOI： 10.3186/jjphytopath.52.53

CiNii Article

CiNii Books

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Regulation of pisatin biosynthesis in pea leaves by elicitor and suppressor produced by Mycosphaerella pinodes 共著

Annals of the Phytopathological Society of JAPAN   52,53-58   1986年

　詳細を見る

DOI： 10.3186/jjphytopath.52.53

researchmap