研究者詳細 - 一瀬　勇規

MISC - 一瀬　勇規

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Con A-binding protein in plasma membranes may participate in signal-transduction cascades that lead to defense responses in pea.

K Toyoda, M Sugimoto, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

PLANT PHYSIOLOGY 114 ( 3 ) 1502 - 1502 1997年7月

Association between ion fluxes and defense responses in pea and cowpea tissues

M Amano, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY 38 ( 6 ) 698 - 706 1997年6月

記述言語：英語出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS

The glycopeptide elicitor from a pea pathogen, Mycosphaerella pinodes, induced rapid alkalinization and increases in levels of Na+ and K+ ions in the extracellular solution upon contact with pea and cowpea tissues, The presence of monensin, nigericin, lidocaine, quinidine or phenytoin together with the elicitor markedly inhibited these changes, whereas the presence of valinomycin, gramicidin D, tetraethylammonium, CsCl and aminopyridine did not. The production of phytoalexins in pea and cowpea tissues was also strongly inhibited by the simultaneous presence of the former reagents but not of the latter reagents. Inhibitory effects on the production of phytoalexins were diminished when monensin, nigericin or a Na+-channel blocker was applied 3 h after the start of treatment with elicitor, Furthermore, orthovanadate and neomycin, which suppress defense responses in both tissues, also inhibited the above mentioned changes. By contrast, the species-specific suppressor from M. pinodes inhibited the elicitor-induced release of Na+ and K+ ions from pea tissues, but, conversely, by itself it elicited either the defense response or the release of Na+ and K+ ions from cowpea tissues. The results indicate that these ion-related changes, in particular the efflux of Na+ and K+ ions, might be closely associated with the signal transduction system for defense responses at the tissue level.

DOI： 10.1093/oxfordjournals.pcp.a029223

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Molecular evolution and functional relevance of the chalcone synthase genes of pea

M Ito, Y Ichinose, H Kato, T Shiraishi, T Yamada

MOLECULAR & GENERAL GENETICS 255 ( 1 ) 28 - 37 1997年6月

記述言語：英語出版者・発行元：SPRINGER VERLAG

We have isolated seven genomic chalcone synthase (CHS) genes and six classes of CNS cDNA from elicitor-treated pea tissues. Comparison of the nucleotide sequences of the coding regions revealed the existence of eight members of the CHS gene family in pea. These can essentially be divided into three groups (PSCHS1, 2 and 8; PSCHS3, 4 and 5; and PSCHS6 and 7) on the basis of nucleotide and/or amino acid sequence comparisons of the coding regions, introns and promoter regions. We previously reported that the accumulation of CHS mRNAs is induced by elicitor treatment. Accumulation of CHS mRNA was observed mainly in roots and very little was found in floral organs. To specifically detect expression of each CHS gene in various types of pea cells, S1 nuclease protection assays were performed. Interestingly, the classification of the eight members of the CHS gene family based on the sequence identity was found to reflect their expression patterns as determined by the S1 nuclease protection assay. The first group of CHS genes, PSCHS1, 2 and 8, was strongly induced not only by elicitor treatment and UV irradiation but is also constitutively expressed in root and flower tissues. The second group, PSCHS3, 4 and 5, was also strongly induced by elicitor treatment and UV irradiation but is constitutively expressed only in root. Expression of the third group, PSCHS6 and 7 was barely detectable in any of the organs tested and was not influenced by environmental stimuli such as elicitor or UV. Furthermore, sequence analysis of the promoter region of each member of the CHS gene family revealed that putative cis-regulatory elements, such as Box-I, Box-II and G-Box, were conserved only in PSCHS1, 2, 3, 4 and 5. From these results we propose that an ancestral CHS gene might have given rise to defense response-related (UV irradiation- and elicitor-responsive) and -unrelated (unresponsive) genes at an early stage of evolution, followed by divergence within these subclasses based upon the developmental program in pea.

DOI： 10.1007/s004380050471

dad-1, a putative programmed cell death suppressor gene in rice

Y Tanaka, T Makishima, M Sasabe, Y Ichinose, T Shiraishi, T Nishimoto, T Yamada

PLANT AND CELL PHYSIOLOGY 38 ( 3 ) 379 - 383 1997年3月

記述言語：英語出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS

The human dad-1 cDNA homolog was isolated from rice plants. The amino acid sequence of the predicted protein product is well conserved in both animals and plants. This rice dad-1 homolog can rescue the temperature-sensitive dad-1 mutants of hamster cells from apoptotic death, suggesting that the rice dad-1 homolog also functions as a suppressor for programmed cell death.

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RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS -SIGNIFICANCE OF CELL WALL-

SUGIURA Tetsuya, KIBA Akinori, AOYAGI Masaaki, KATO Toshiaki, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology 38 s58 1997年3月

THE SIGNIFICANCE OF ACTIVE OXYGEN SPEACIES IN ELICITATION OF DEFENSE RESPONSES IN PEA PLANTS

INATA Atsuko, FUJII Masahiro, KIBA Akinori, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology 38 s92 1997年3月

EFFECTS OF SYNTHETIC GLYCOPEPTIDE-ELICITORS ON DEFENSE RESPONSES OF PEA PLANTS

KIBA Akinori, INATA Atsuko, TAKEDA Tadahiro, KANEMITSU Takuya, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology 38 s93 1997年3月

PLANT CELL WALL - THE PRIMARY APPARATUS RECOGNIZING AND RESPONDING TO FUNGAL SIGNALS

SHIRAISHI Tomonori, YAMADA Tetsuji, ICHINOSE Yuki, KIBA Akinori

Plant and cell physiology 38 s11 1997年3月

Combined effects of multiple cis-acting elements in elicitor-mediated activation of PSCHS1 gene

H Seki, Y Ichinose, M Ito, T Shiraishi, T Yamada

PLANT AND CELL PHYSIOLOGY 38 ( 1 ) 96 - 100 1997年1月

記述言語：英語出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS

Promoter of a gene encoding chalcone synthase 1 (PSCHS1), a member of the defense-related genes in pea, was analyzed by transient transfection assay. The results demonstrated that in addition to the previously identified AT-rich sequences, at least four distinct cis-acting elements were required for maximal fungal elicitor-mediated activation of PSCHS1.

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バナジン酸によるエンドウ培養細胞におけるフィトアレキシン生産の誘導(英文)

河原智治, 豊田和弘, 木場章範, 一瀬勇規, 山田哲治, 白石友紀

岡山大学農学部学術報告 86 33 - 41 1997年

Association between ion fluxes and defense responses in pea and cowpea tissues(共著)

Plant Cell Physiology 38 ( 6 ) 698 - 706 1997年

Temporal and spatial pattern of expression of the pea phenylalanine ammoria-lyase gene 1 promoter in transgenic tobacco(共著)

Plant Cell Physiology 38 ( 7 ) 792 - 803 1997年

The Effects of Expression of Procaryotic-type iaa Genes inAgrobacterium tumefaciens on Plant Tumorigenicity.

村上泰弘, 西野友規, 平嗣良, YUNUS Ahmad, 一瀬勇規, 白石友紀, 山田哲治

日本植物病理学会報 63 ( 5 ) 377 - 380 1997年

記述言語：英語出版者・発行元：The Phytopathological Society of Japan

植物関連細菌が持つ原核型iaa遺伝子の役割を調べるために,オリーブこぶ病菌(Pseudomonas syringae pv. savastanoi)のiaaオペロンを広宿主範囲ベクターpRK415に導入した組み換え体プラスミドpTOY1を構築し,これを根頭癌腫病菌(Agrobacterium tumefaciens A208)に導入した。A. t. A208は感染した植物細胞でのみIAAを生産するのに対し,組み換え体A. t. A208 (pTOY1)はそれとともに恒常的にIAAを生産する.組み換え体A. t. A208(pTOY1)を接種したトマト茎にはA. t. A208に比べて大きなクラウンゴールが形成され,さらにポテトディスクでもカルス誘導が促進された。一方,A. t. A208あるいはA. t. A208 (pTOY1)をポテトディスクに接種した後,植物防御応答に関連するphenylalanine ammonia-lyaseの酵素活性を経時的に調べると,A. t. A208よりもA. t. A208 (pTOY1)接種でポテトのPAL活性が低下した。したがって, A. t. A208 (pTOY1)の強病原性は, A. t. A208 (pTOY1)によって生産されたIAAによって感染現場で植物防御応答が抑制された結果であると考えられた。