研究者詳細 - 一瀬　勇規

MISC - 一瀬　勇規

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Methyl jasmonate inhibits Harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of defense genes in tobacco suspension cultured BY-2 cells.

Plant Cell Physiol. 42 ( 4 ) 446 - 449 2001年

Harpin_<psta> from Pseudomonas syringae pv. tabaci Is Defective and Deficient in Its Expression and HR-inducing Activity :

TAGUCHI Fumiko, TANAKA Rui, KINOSHITA Sayuri, ICHINOSE Yuki, IMURA Yoshiyuki, ANDI Salamah, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general plant pathology : JGPP 67 ( 2 ) 116 - 123 2001年

記述言語：英語出版者・発行元：Phytopathological Society of Japan

To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P.s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using recombinant harpin indicated harpin_<psta> was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P.s. pathovars in hrp-inducing medium, the truncated harpin_<psta> was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant of the host-parasite specificity in P.s. pv. tabaci. (Received August 10, 2000 ; Accepted December 21, 2000)

DOI： 10.1007/PL00012997

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その他リンク： http://id.nii.ac.jp/1141/00138222/

Gramine increase associated with rapid and transient systemic resistance in barley seedlings induced by mechanical and biological stresses.

Matsuo, H, Taniguchi, K, Hiramoto, T, Yamada, T, Ichinose, Y, Toyoda, K, Takeda, K, Shiraishi, T

Plant Cell Physiol. 42 ( 10 ) 1103 - 1111 2001年

Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

K Toyoda, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

JOURNAL OF PHYTOPATHOLOGY 148 ( 11-12 ) 633 - 636 2000年12月

記述言語：英語出版者・発行元：WILEY-BLACKWELL

When epicotyl tissues of pea were treated with a diacylglycerol (DAG) kinase inhibitor (R59022), enhanced induction of the phytoalexin accumulation occurred which was induced by fungal elicitor. The marked induction was associated with a sustained accumulation of phenylalanine ammonia-lyase (PAL)-mRNA and the consequent increase in PAL activity. These results suggest that inhibition of DAG breakdown leads to increased induction of phytoalexin accumulation and support the hypothesis that DAG kinase negatively regulates the signal transduction.

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Phytoalexin accumulation in elicitor-treated epicotyis of pea(Pisum sativum L.)by a diacylglycerol kinase inhibitor.(共著)

Journal of Phytopathology 148 ( 11-12 ) 633 - 636 2000年12月

Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture

M Sasabe, K Takeuchi, S Kamoun, Y Ichinose, F Govers, K Toyoda, T Shiraishi, T Yamada

EUROPEAN JOURNAL OF BIOCHEMISTRY 267 ( 16 ) 5005 - 5013 2000年8月

記述言語：英語出版者・発行元：BLACKWELL SCIENCE LTD

We characterized pharmacologically the hypersensitive cell death of tobacco BY-2 cells that followed treatments with Escherichia coli preparations of INF1, the major secreted elicitin of the late blight pathogen Phytophthora infestans. INF1 elicitin treatments resulted in fragmentation and 180 bp laddering of tobacco DNA as early as 3 h post-treatment. INF1 elicitin also induced rapid accumulation of H2O2 typical of oxidative burst, and the expression of defense genes such as phenylalanine ammonia-lyase (PAL) gene at 1 h and 3 h after elicitin treatment, respectively. To investigate the involvement of the oxidative burst and/or the expression of defense genes in the signal transduction pathways leading to hypersensitive cell death, we analyzed the effect of several chemical inhibitors of signal transduction pathways on the various responses. The results indicated that (a) the cell death required serine proteases, Ca2+ and protein kinases, (b) the oxidative burst was involved in Ca2+ and protein kinase mediated pathways, but elicitin-induced AOS was neither necessary nor sufficient for cell death and PAL gene expression, and (c) the signaling pathway of PAL gene expression required protein kinases. These results suggest that the three signal transduction pathways leading to cell death, oxidative burst and expression of defense genes branch in the early stages that follow elicitin recognition by tobacco cells.

DOI： 10.1046/j.1432-1327.2000.01553.x

Characterization of cDNAs encoding two glycine-rich proteins in chickpea (Cicer arietinum L.) : Accumulation in response to fungal infection and other stress factors.(共著)

CORNELS H, ICHINOSE Y, BARZ W

Plant Science 154 ( 1 ) 83 - 88 2000年5月

Cytochalasin A inhibits the binding of phenylalanine ammonia-lyase mRNA to ribosomes during induction of phytoalexin in pea seedlings

M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY 41 ( 2 ) 234 - 238 2000年2月

記述言語：英語出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS

Cytochalasin A (CA) blocked the accumulation of phytoalexin and phenylalanine ammonia-lyase (PAL)-protein in pea tissues treated with a fungal elicitor but scarcely affected the PAL-mRNA content. Further analysis showed that CA decreased the PAL-mRNA bound to ribosomes. These results indicate that actin filaments are tightly associated with the translational process of the PAL gene.

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Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization.(共著)

Zeitschrift fuer Naturforschung 55 ( 1-2 ) 44 - 54 2000年1月

lmportance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression lnduced by Nonpathogen Attack.(共著)

IMURA Y, IGUCHI S, TOYODA K, ICHINOSE Y, SHIRAISHI T, YAMADA T

Journal of General Plant Pathol.ogy 66 ( 2 ) 123 - 127 2000年

記述言語：英語出版者・発行元：Phytopathological Society of Japan

Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

DOI： 10.1007/PL00012932

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その他リンク： http://id.nii.ac.jp/1141/00138133/

Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

K. Toyoda, T. Kawahara, Y. Ichinose, T. Yamada, T. Shiraishi

Journal of Phytopathology 148 ( 11-12 ) 633 - 636 2000年

記述言語：英語

DOI： 10.1046/j.1439-0434.2000.00568.x

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Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (┣DB Pisum sativum (/)-┫DB L.) by a diacylglycerol kinase inhibitor.

J. Phytopathol. 148 ( 11-12 ) 633 - 636 2000年

lndependent pathways leading to apoptotic cell death,oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture.(共著)

SASABE M, TAKEUCHI K, ICHINOSE Y, TOYODA K, SHIRAISHI T, YAMADA T, KAMOUN S, GOVERS F

European Journal of Biochemistry 267 ( 16 ) 5005 - 5013 2000年

Cytochalasin A inhibits the binding of phenylalanine ammonia-lyass mRNA to ribosomes during induction of phytoalexin in pea seedlings.(共著)

Plant Cell Physiology 41 234 - 238 2000年

Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack :

IMURA Yoshiyuki, IGUCHI Satoko, TOYODA Kazuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general plant pathology : JGPP 66 ( 2 ) 123 - 127 2000年

記述言語：英語出版者・発行元：Phytopathological Society of Japan

DOI： 10.1007/PL00012932

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その他リンク： http://id.nii.ac.jp/1141/00138133/

PLANT CELL WALL RECOGNIZES AND RESPONDS TO SIGNALS FROM PHYTOPATHOGENS :

KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology 41 s10 2000年

STRUCTURAL AND FUNCTIONAL ANALYSES OF HARPINS FROM SEVERAL PATHOVARS OF PSEUDOMONAS SYRINGAE :

TANAKA Rui, TAGUCHI Tomiko, KINOSHITA Sayuri, NAKADA Hideki, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology 41 s84 2000年

植物病原菌と防御機構 (環境応答・適応の分子機構) -- (植物の環境応答と耐性のメカニズム)

山田哲治, 白石友紀, 一瀬勇規

蛋白質核酸酵素 44 ( 15 ) 2308 - 2314 1999年11月

Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes

A Kiba, T Takeda, T Kanemitsu, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY 40 ( 9 ) 978 - 985 1999年9月

記述言語：英語出版者・発行元：OXFORD UNIV PRESS

A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of beta-D-Glc-(1-->6)-alpha-D-Man-(1-->6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including beta-D-Glc-(1-->6)-alpha-D-Man-(1-->6)-D-Man-O-Ser (No. 1) to [beta-D-Glc-(1-->6)-alpha-D-Man-(1-->6)-D-Man](3)-O-Ser(3)-Pro(3) (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycopeptides [beta-D-Glc-(1-->6)-alpha-D-Man-(1-->6)-D-Man]-O-Ser(2)-Pro(2) (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

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Functional analysis of retrotransposons in pea

H Kato, P Sriprasertsak, H Seki, Y Ichinose, T Shiraishi, T Yamada

PLANT AND CELL PHYSIOLOGY 40 ( 9 ) 933 - 941 1999年9月

記述言語：英語出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS

The 5'-upstream regions of the plant active defense genes in pea (PSPAL2 and PSCHS1) exhibit significant nucleotide sequence identity to part of a copia-type retrotransposon. To characterize the retrotransposon in pea putative reverse transcriptase sequences (Psr) were amplified by PCR from cDNA prepared from protoplasts derived from pea suspension cultured cells. Psr genes can be classified into at least eight subgroups (A-H) according to their nucleotide sequence similarities. A subgroup PsrC was induced by the treatment of etiolated pea epicotyls with fungal elicitor within 30 min but was hardly induced by protoplasting of pea suspension cultured cells, whereas subgroups of PsrA and PsrB were highly induced by protoplasting. Interestingly, the retrotransposon sequence present at the 5'-upstream region of PSCHS1 which was induced by the treatment with fungal elicitor belonged to subgroup PsrC. The retrotransposon might play an important role in reorganization of the genes and optimization of the gene expression in response to various environmental stimuli such as an attack by phytopathogens or protoplasting.

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