研究者詳細 - 豊田　和弘

MISC - 豊田　和弘

分割表示 359 件中 241 - 260 件目／全件表示 >>

Effect of harpin from four pathovars of ┣DB Pseudomonas syringae (/)-┫DB on pea defense responses.

TANAKA R, TAGUCHI F, ICHINOSE Y, TOYODA K, SHIRAISHI T, YAMADA T

J. Gen. Plant Pathol. 67 ( 2 ) 148 - 151 2001年

記述言語：英語出版者・発行元：Phytopathological Society of Japan

To investigate the role of the proteinaceous elicitor, harpin, on host and nonhost plants, we isolated the harpin-coding gene, hrpZ, from Pseudomonas syringae pvs. pisi, glycinea, tabaci and tomato. Effects of the recombinant harpin proteins on pea plants were analyzed and compared with the effects of the corresponding bacterial treatment. After inoculation of pea with pea pathogen P. syringae pv. pisi, the bacterial population increased and the accumulation of PAL-mRNA and pisatin was inhibited. The nonpathogenic pathovars, glycinea, tabaci and tomato induced both defense responses in pea. However, none of the harpins induced the hypersensitive reaction or accumulation of PAL-mRNA and pisatin in pea. Harpins from P. syringae pvs. glycinea, tomato and pisi did induce these defense responses in tobacco, however, suggesting that externally applied harpins either are not recognized or are nonfunctional in pea plants. (Received July 27, 2000 ; Accepted February 21, 2001)

DOI： 10.1007/PL00013001

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その他リンク： http://id.nii.ac.jp/1141/00138226/

Effect of methyl jasmonate on harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of defense genes in tobacco suspension cultured BY-2 cells.

Andi, S, Taguchi, F, Toyoda, K, Shiraishi, T, Ichinose, Y

Plant Cell Physiol. 42 ( 4 ) 446 - 449 2001年

HarpinPsta from Pseudomonas syringae pv. tabaci is defective and deficient in its expression and HR-inducing activity.

Taguchi, F, Tanaka, R, Kinoshita, S, Ichinose, Y, Imura, Y, Andi, S, Toyoda, K, Shiraishi, T, Yamada, T

J. Gen. Plant Pathol. 67 ( 2 ) 116 - 123 2001年

初期・表層シグナル伝達系と防御システム

白石友紀, 豊田和弘, 木場章範, 一瀬勇規

化学と生物 39 (10): 686-692 ( 10 ) 686 - 692 2001年

タバコ培養細胞における活性酸素種生成と過敏感反応(関西部会講演要旨)

竹内香純, 笹部美知子, 一瀬勇規, 豊田和弘, 白石友紀, 山田哲治

日本植物病理學會報 66 ( 3 ) 287 - 288 2000年12月

Pseudomonas syringaeの生産する過敏感反応誘導因子 : (2) harpinによる過敏感反応誘導と抑制機構(関西部会講演要旨)

Andi S., 田口富美子, 土居玲奈, 田中塁, 笹部美知子, 豊田和弘, 白石友紀, 山田哲治, 一瀬勇規

日本植物病理學會報 66 ( 3 ) 292 - 293 2000年12月

エンドウ細胞壁NTPaseは病原菌シグナル分子を認識する(関西部会講演要旨)

吉岡一顕, 木場章範, 河原智治, 豊田和弘, 一瀬勇規, 山田哲治, 白石友紀

日本植物病理學會報 66 ( 3 ) 288 - 288 2000年12月

Pseudomonas syringaeの生産する過敏感反応誘導因子 : (3) エリシターとしてのflagellinの単離と機能解析(関西部会講演要旨)

田口富美子, 中島梨衣, Andi s., 豊田和弘, 白石友紀, 山田哲治, 一瀬勇規

日本植物病理學會報 66 ( 3 ) 293 - 293 2000年12月

エンドウにおけるサプレッサー応答性遺伝子の解析 : (3)S64タンパク質はoxophytodienoic acid reductaseである(関西部会講演要旨)

石賀康博, 一瀬勇規, 豊田和弘, 白石友紀, 山田哲治

日本植物病理學會報 66 ( 3 ) 288 - 288 2000年12月

病原菌シグナル伝達における細胞壁-原形質膜間の相互作用 : -インテグリン様分子とホスファチジルイノシトール1リン酸リン酸化酵素(PIP5K)の相互作用について-(関西部会講演要旨)

豊田和弘, 岡崎愛理, 一瀬勇規, 山田哲治, 白石友紀

日本植物病理學會報 66 ( 3 ) 288 - 288 2000年12月

Pseudomonas syringaeの生産する過敏感反応誘導因子 : (1)harpinの構造と機能解析(関西部会講演要旨)

田中塁, 田口富美子, Andi S., 木下さゆり, 豊田和弘, 白石友紀, 山田哲治, 一瀬勇規

日本植物病理學會報 66 ( 3 ) 292 - 292 2000年12月

Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

K Toyoda, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

JOURNAL OF PHYTOPATHOLOGY 148 ( 11-12 ) 633 - 636 2000年12月

記述言語：英語出版者・発行元：WILEY-BLACKWELL

When epicotyl tissues of pea were treated with a diacylglycerol (DAG) kinase inhibitor (R59022), enhanced induction of the phytoalexin accumulation occurred which was induced by fungal elicitor. The marked induction was associated with a sustained accumulation of phenylalanine ammonia-lyase (PAL)-mRNA and the consequent increase in PAL activity. These results suggest that inhibition of DAG breakdown leads to increased induction of phytoalexin accumulation and support the hypothesis that DAG kinase negatively regulates the signal transduction.

DOI： 10.1046/j.1439-0434.2000.00568.x

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細胞壁ATPaseに対する褐紋病菌サプレッサーの作用(6) : 病原菌シグナル結合タンパク質遺伝子のクローニングと機能解析(平成12年度日本植物病理学会大会講演要旨)

木場章範, 大河原多佳子, 豊田和弘, 一瀬勇規, 山田哲治, 白石友紀

日本植物病理學會報 66 ( 2 ) 116 - 117 2000年8月

Cytochalasin A inhibits the binding of phenylalanine ammonia-lyase mRNA to ribosomes during induction of phytoalexin in pea seedlings

M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY 41 ( 2 ) 234 - 238 2000年2月

記述言語：英語出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS

Cytochalasin A (CA) blocked the accumulation of phytoalexin and phenylalanine ammonia-lyase (PAL)-protein in pea tissues treated with a fungal elicitor but scarcely affected the PAL-mRNA content. Further analysis showed that CA decreased the PAL-mRNA bound to ribosomes. These results indicate that actin filaments are tightly associated with the translational process of the PAL gene.

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Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization

Y Ichinose, K Tiemann, C Schwenger-Erger, K Toyoda, F Hein, T Hanselle, H Cornels, W Barz

ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES 55 ( 1-2 ) 44 - 54 2000年1月

記述言語：英語出版者・発行元：VERLAG Z NATURFORSCH

In response to the exogenous application of elicitors and attempted invasion by pathogens, plants exhibit a wide range of defense reactions. To understand the defense mechanisms at the level of gene activation and deactivation, differential screenings were performed to isolate cDNA clones which are differentially expressed in pathogen-inoculated resistant chickpea plants and elicitor-treated cell cultures. A plenty of genes were isolated and arranged in 5 groups, namely defense-related pathways, signal transduction pathways, regulation of gene expression, catabolic pathways and primary metabolism. Most of these genes were activated although several genes were also found to be suppressed. We discuss the plausible functions of cDNA products in plant defense responses. The cDNAs provide a variety of tools to investigate molecular mechanisms of defense responses and clearly reflect the massive genomic and metabolic changes which occur during manifestation of antimicrobial defense.

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Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack :

IMURA Yoshiyuki, IGUCHI Satoko, TOYODA Kazuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general plant pathology : JGPP 66 ( 2 ) 123 - 127 2000年

記述言語：英語出版者・発行元：Phytopathological Society of Japan

Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

DOI： 10.1007/PL00012932

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その他リンク： http://id.nii.ac.jp/1141/00138133/

PLANT CELL WALL RECOGNIZES AND RESPONDS TO SIGNALS FROM PHYTOPATHOGENS :

KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology 41 s10 2000年