Faculty Profiles - OTSUKA Fumio

Papers - OTSUKA Fumio

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Renal AT1 receptor: autoradiographic localization and quantification in rat

N Asano, T Ogura, Y Mimura, M Kishida, H Kataoka, F Otsuka, T Yamauchi, H Makino

Res. Commun. Mol. Pathol. Pharmacol. 100 ( 2 ) 161-170 - 170 1998.1

Language：English Publishing type：Research paper (scientific journal) Publisher：P J D PUBLICATIONS LTD

To elucidate the precise localization of angiotensin II (Ang II) type 1 (AT(1)) receptor in the kidney, we utilized in vitro macro- and micro-autoradiography (ARG) of [H-3]-Ang II bindings to the Wistar rat kidney in the presence of L158,809, a specific non-peptide AT(1) receptor antagonist. Besides, we estimated the density of renal AT, receptors using the quantification of macro-ARG. The density of [H-3]-Ang II binding to renal tissue was concentration-dependent in both renal cortex and medulla Although the addition with 500 nM arginine vasopressin and 500 nM atrial natriuretic peptide had no effect on [H-3]-Ang II, the total binding of [H-3] Ang II completely displaced by the addition with 500 nM unlabeled Ang II or L-158,809. Macro-ARG revealed that the amount of both Ang II and AT, receptors in the renal medulla gready exceeded those in the renal cortex In the medulla, the density of these receptors was not localized on the outer medulla but was confirmed mainly to the inner medulla, especially to the inner zone and longitudinal bands. Since the density and localization of AT(1) receptors was consistent with that of total Ang II receptors, it appears that AT(1) receptors comprise most of the Ang II receptors in the kidney. Micro-ARG revealed that Ang II receptors were mainly located in the glomerulus and proximal tubules of the renal cortex, as well as on the circumferences of vessels and the vasa recta of the renal medulla. The present study established a method for ARO of AT(1) receptors in the kidney as well as a method for quantifying the macro-ARG.

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Renal AT1 receptor: computerized quantification in spontaneously hypertensive rats and DOCA-salt rats

N Asano, T Ogura, Y Mimura, F Otsuka, M Kishida, M Hashimoto, T Yamauchi, H Makino

Res. Commun. Mol. Pathol. Pharmacol. 100 ( 2 ) 171-180 - 180 1998.1

Language：English Publishing type：Research paper (scientific journal) Publisher：P J D PUBLICATIONS LTD

To assess the involvement of angiotensin II (Ang II) in the regulation of blood pressure, we investigated the alterations of renal Ang II type 1 (AT(1)) receptors in two different models of hypertension; i.e., in spontaneously hypertensive rats (SHR) and deoxycorticosterone acetate (DOCA)-salt hypertensive rats by using the method for quantification of in vitro macroautoradiography (ARG). In the SHR model, although the number of cortical AT(1) receptors equaled that of Wistar Kyoto (WKY) rats at 4 and 12 weeks of age, the number of medullary AT(1) receptors in the 4-week-old SHR animals was significantly lower than that in age-matched WKY rats, and increased by 12 weeks of age. The renal AT(1) receptor number in DOCA-salt hypertensive rats was significantly higher than that in control rats. The amount of these receptors also increased with age in both DOCA-salt hypertensive rats and control rats. These findings indicate that the development of medullary AT(1) receptors in early stages of hypertension in the SHR model differs from that in WKY rats or DOCA-salt hypertensive rats. This suggests that renal AT(1) receptors may contribute to the hypertension seen in the SHR group. The renal AT(1) receptors appear to be up-regulated in early stages of DOCA-salt-induced hypertension as well.

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Reversible Hypothyroidism in Empty Sella Syndrome: A Case Report.

FUMIO OTSUKA, TOSHLO OGURA, NOBUHIKO HAYAKAWA, SEISHI HARADA, JINGO KAGEYAMA, HIROFUMI MAKINO

Endocrine Journal 45 ( 3 ) 385 - 391 1998

Cushing's Syndrome due to Unilateral Adrenocortical Hyperplasia.

Fumio OTSUKA, Toshio OGURA, Kazushi NAKAO, Nobuhiko HAYAKAWA, Yukari MIMURA, Takayoshi YAMAUCHI, Hirofumi MAKINO

Internal Medicine 37 ( 4 ) 385 - 390 1998

Hypocalcemia due to Spontaneous Infarction of Parathyroid Adenoma and Osteomalacia in a Patient with Primary Hyperparathyroidism.

FUMIO OTSUKA, TOSHIO OGURA, TORU SATO, NOBUHIKO HAYAKAWA, YUKARI MIMURA, MASAYUKI KISHIDA, TAKAYOSHI YAMAUCHI, HIROFUMI MAKINO

Endocrine Journal 45 ( 5 ) 617 - 623 1998

Pituitary Apoplexy Induced by a Combined Anterior Pituitary Test: Case Report and Literature Review.

FUMIO OTSUKA, JINGO KAGEYAMA, TOSHLO OGURA, HIROFUMI MAKINO

Endocrine Journal 45 ( 3 ) 393 - 398 1998

Sheehan's Syndrome of More Than 30 Years' Duration: An Endocrine and MRI Study of 6 Cases.

FUMIO OTSUKA, JINGO KAGEYAMA, TOSHIO OGURA, TERUHIKO HATTORI, HIROFUMI MAKINO

Endocrine Journal 45 ( 4 ) 451 - 458 1998

Effects of OPC-21268, a vasopressin V1-receptor antagonist, on expression of growth factors from glomeruli in spontaneously hypertensive rats

Fumio Otsuka, Toshio Ogura, Takayoshi Yamauchi, Tetsuya Oishi, Masami Hashimoto, Yukari Mimura, Hirofumi Makino

Regulatory Peptides 72 ( 2-3 ) 87 - 95 1997.10

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

To assess the chronic in vivo effects of OPC-21268, a vasopressin-Vl receptor antagonist, on renal injury, we investigated the mRNA expressions of platelet-derived growth factor (PDGF) B-chain, transforming growth factor (TGF)-beta 1 and proliferating cell nuclear antigen (PCNA) in the glomeruli of spontaneously hypertensive rats (SHR) treated with OPC-21268 for 3 weeks. SHR aged 10 weeks were given 2% NaCl in drinking water for 3 weeks. The OPC group was fed a 0.5% OPC-21268-containing diet for 3 weeks and the control group was given a normal diet. There were no significant changes in the time course of systolic blood pressure, heart rate, urine volume, or urinary sodium, protein and N-acetyl-beta-glucosaminidase (NAG) excretion between the two groups. Serum electrolytes, protein and creatinine levels also did not differ between the groups. The mRNA expressions of PDGF B-chain, TGF-beta 1 and PCNA in the glomerulus were examined using reverse transcriptase-polymerase chain reaction (RT-PCR) methods. The mRNA expressions of PDGF B-chain and PCNA among these were significantly suppressed in the OPC group. No significant differences in renal histology including the organ weights were found between the two groups; however, the glomerular size tended to be enlarged in the OPC group. These findings suggest that chronic V1-receptor blockade directly inhibits the glomerular proliferative injury of salt-loaded SHR at the established hypertension stage. (C) 1997 Elsevier Science Ireland Ltd.

DOI： 10.1016/s0167-0115(97)01041-0

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Postanesthetic Polyuria Attributable to Central Diabetes Insipidus

Fumio Otsuka, Satoshi Mizobuchi, Kiyoshi Morita, Hideo Matsumoto, Toshio Ogura, Masahisa Hirakawa, Hirofumi Makino

Anesthesia & Analgesia 85 ( 4 ) 940 - 943 1997.10

In vitro Macro-and Microautoradiographic Localization of V1 and V2 Receptors in the Rat Kidney Using OPC-21268 and OPC-31260 Reviewed

Yukari Mimura, Toshio Ogura, Nobuhiko Hayakawa, Fumio Otsuka, Masami Hashimoto, Takayoshi Yamauchi, Hirofumi Makino, Norio Ogawa

Nephron 76 ( 3 ) 331 - 336 1997

Language：English Publishing type：Research paper (scientific journal)

To elucidate the precise localization of vasopressin(VP) Vj and V2 receptors in the kidney, we utilized in vitro macroautoradiography (macro-ARG) and microautoradiography (micro-ARG) of these receptors in Wistar rat kidneys.This was done by using OPC-21268 and OPC-31260, two newly developed selective Vi (OPC-21268) and V2 (OPC-31260)receptor antagonists. For macro-ARG, 10-μm kidney sections were incubated with Tris-HCl buffer containing [3H]-VP with or without unlabeled ligand (VP, OPC-21268, orOPC-31260) at 20°C for 40 min. These sections were then loaded intoX-ray cassettes with Hyperfilm-[3H] and exposed inthe dark for 2 months. The autoradiograms were quantitativelyanalyzed by using the research analysis system RAS 1,000
the V1 and V2 receptors were quantitated by inftracting the nonspecific binding (incubatedwith OPC-21268 and OPC-31260, respectively) from the total binding. To assess a more precise localization of the V1and V2 receptors, we also investigated the micro-ARG of the renal V1 and V2 receptorsby dipping the kidney section slides used for macro-ARG into a photographic emulsion and observing the receptors under light microscopy. [3H]-VP binding to the rat kidney was completely displaced by unlabeled excess VP, but not by unlabeled angiotensin II, indicating that [3H]-VP binding was specific for VP receptors. Computerized quantification showed that V2 receptors, visualized by OPC-31260, were the predominant type of VP receptor in the kidney. Conversely, V1receptors, visualized by OPC-21268, werefewer in number. V) receptors were partly localized to the glomerulus, cortical vessels, interstitial cells, and the medullary vessels. The V2receptors localized to the collecting ducts and medullary tubules. Our findings indicated that renal V1 and V2 receptors can be detected by in vitro macro- and micro-ARG by using OPC-21268 and OPC-31260. © 1997 S.Karger AG, Basel.

DOI： 10.1159/000190200

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IDDM accompanied a growth hormone-producing pituitary adenoma: A case report Reviewed

Fumio Otsuka, Toshio Ogura, Takayoshi Yamauchi, Kenichi Shikata, Jingo Kageyama, Hirofumi Makino

Diabetes Care 20 ( 12 ) 1838 - 1841 1997

Language：English Publishing type：Research paper (scientific journal) Publisher：American Diabetes Association Inc.

CASE HISTORY - A 30-year-old Japanese man who presented with recurrent ketoacidosis caused by IDDM was found to have increased secretion of growth hormone (GH). On initial cranial magnetic resonance imaging (MRI), no pituitary lesion was detected
however, a pituitary microadenoma was found 2 years later during a repeat MRI. In spite of the hypersecretion of GH, serum IGF-I was dramatically suppressed. Transsphenoidal surgery was performed to resect the pituitary tumor that was histologically an acidophilic pituitary adenoma. Although the GH excess rapidly improved postoperatively, the IGF-I level remained low. Subsequent insulin therapy initiated 1 year after the operation elevated the serum IGF-I level to within the normal range. DISCUSSION - The first case of coexistent IDDM and a GH-producing pituitary adenoma suggests that patients with uncontrolled IDDM may develop GH hypersecretion. Furthermore, the low IGF-I levels may be closely associated with the GH excess and with the development or progression of GH-secreting pituitary adenomas.

DOI： 10.2337/diacare.20.12.1838

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Manifestation of Primary Hyperthyroidism after Pituitary Adenomectomy: A Case Report.

FUMIO OTSUKA, TOSHIO OGURA, NOBUHIKO HAYAKAWA, YUKARI MIMURA, JINGO KAGEYAMA, HIROFUMI MAKINO

Endocrine Journal 44 ( 6 ) 887 - 893 1997

A Case of Primary Hyperparathyroidism Accompanying Multiple Myeloma.

FUMIO OTSUKA, NOBUHIKO HAYAKAWA, TOSHIO OGURA, HIDEO KATAOKA, MASANORI KAWASHIMA, YUKARI MIMURA, TAKAYOSHI YAMAUCHI, NAOKI KASHIHARA, JINGO KAGEYAMA, HIROFUMI MAKINO

Endocrine Journal 44 ( 1 ) 105 - 109 1997

Gestational Thyrotoxicosis Manifesting as Wernicke Encephalopathy: A Case Report.

FUMIO OTSUKA, KATSUHIKO TADA, TOSHIO OGURA, NOBUHIKO HAYAKAWA, YUKARI MIMURA, TAKAYOSHI YAMAUCHI, NANAKO INOUE, HIROFUMI MAKINO, TAKAFUMI KUDO

Endocrine Journal 44 ( 3 ) 447 - 452 1997

静脈疾患とリンパ管外科：深部静脈血栓症

柚木継二、末広晃太郎、内田發三、*大塚文男、天野哲基、槇野博史、太田善介

1997

Changes in urinary enzyme activity and histochemical findings in experimental tubular injury by gold sodium thiomalate

Toshio Ogura, Michio Takaoka, Takayoshi Yamauchi, Tetsuya Oishi, Yukari Mimura, Masami Hashimoto, Naoko Asano, Masahiro Yamamura, Fumio Otsuka, Hirofumi Makino, Zensuke Ota and Kayo Takahashi

J.Med. 27 41-55 1996.1

Syndrome of Inappropriate Secretion of Antidiuretic Hormone Following Intra-thoracic Cisplatin.

Fumio OTSUKA, Yoshiko HAYASHI, Toshio OGURA, Nobuhiko HAYAKAWA, Shuji IKEDA, Hirofumi MAKING, Zensuke OTA

Internal Medicine 35 ( 4 ) 290 - 294 1996

A Case of Schmidt Syndrome Accompanied by a Pituitary Adenoma.

FUMIO OTSUKA, TOSHIO OGURA, NOBUHIKO HAYAKAWA, MASAMI HASHIMOTO, HIROFUMI MAKINO, ZENSUKE OTA, JINGO KAGEYAMA

Endocrine Journal 43 ( 5 ) 495 - 502 1996

Manifestation of Subclinical Diabetes Insipidus due to Pituitary Tumor during Pregnancy.

MASAMI HASHIMOTO, TOSHIO OGURA, FUMIO OTSUKA, TAKAYOSHI YAMAUCHI, YUKARI MIMURA, NOBUHIKO HAYAKAWA, HIROFUMI MAKINO, ZENSUKE OTA, NORIKO SEKI, YUJI HIRAMATSU, NAOFUMI KUDO

Endocrine Journal 43 ( 5 ) 577 - 583 1996

Normotensive Bilateral Pheochromocytoma with Lindau Disease: Case Report.

FUMIO OTSUKA, TOSHIO OGURA, MINORU NAKAGAWA, NOBUHIKO HAYAKAWA, HIDEO KATAOKA, TETSUYA OISHI, HIROFUMI MAKINO

Endocrine Journal 43 ( 6 ) 719 - 723 1996

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