研究者詳細 - 柳　文修

MISC - 柳　文修

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Role of O6-methylguanine-DNA methyltransferase and effect of O6-benzylguanine on the anti-tumor activity of cis-diaminedichloroplatinum(II) in oral cancer cell lines. 国際誌

Yu Maki, Jun Murakami, Jun-ichi Asaumi, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Susumu Kokeguchi, Kazuhiro Fukui, Noriko Kawai, Yoshinobu Yanagi, Masahiro Kuroda, Noriaki Tanaka, Nagahide Matsubara, Kanji Kishi

Oral oncology 41 ( 10 ) 984 - 93 2005年11月

記述言語：英語出版者・発行元：ELSEVIER SCIENCE BV

The DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) modulates the effectiveness of alkylating agents. However, the relationship between MGMT and the sensitivities to other agents has not been explored. In the present study, the association between MGMT expression and the cellular sensitivity to the platinum agent, CDDP was examined in four human oral cancer cell lines. CDDP depleted MGMT protein and mRNA levels in all four cell lines. Two cell lines with low MGMT expression were sensitive to an alkylating agent, N-methyl-N'-nitro-N-nitrosoguanidine and CDDP, whereas two other cell lines with high MGMT expression were resistant to both agents. Furthermore, the addition of the MGMT inhibitor, O6-benzylguanine (O6-BG), invariably enhanced CDDP sensitivity. CDDP depleted MGMT expression, and CDDP sensitivity was enhanced by O6-BG. These results provide valuable information about the relationship between MGMT expression and CDDP sensitivity in oral cancer chemotherapy.

DOI： 10.1016/j.oraloncology.2005.05.011

Bone deformity showing a deep coronoid notch of the mandible in a patient with neurofibromatosis type 1

M Hisatomi, J Asaumi, H Konouchi, Y Yanagi, K Kishi

DENTOMAXILLOFACIAL RADIOLOGY 34 ( 6 ) 380 - 383 2005年11月

記述言語：英語出版者・発行元：BRITISH INST RADIOLOGY

We present a case of a 10-year-old girl with neurofibromatosis type I related skeletal dysplasia. In the present case, a unilocular radiolucency at the left coronoid notch visible on a panoramic radiograph was suspected to be a neurofibroma. However, only the presence of bone deformities was observed on CT. Fat-like tissue adjacent to the bone deformities inside the left mandibular ramus was revealed, and the presence of neurofibroma was unequivocally denied on the MR images. However, biopsy of the lesion suggested neurofibroma. The diagnostic imaging 18 months after the first visit did not revealed a tumourous mass. However, careful follow up is required even though we could not prove the presence the lesion on the basis of imaging.

DOI： 10.1259/dmfr/46427075

Assessment of ameloblastomas using MRI and dynamic contrast-enhanced MRI. 国際誌

Jun-ichi Asaumi, Miki Hisatomi, Yoshinobu Yanagi, Hidenobu Matsuzaki, Yong Suk Choi, Noriko Kawai, Hironobu Konouchi, Kanji Kishi

European journal of radiology 56 ( 1 ) 25 - 30 2005年10月

記述言語：英語出版者・発行元：ELSEVIER IRELAND LTD

We retrospectively evaluated magnetic resonance images (MRI) and dynamic contrast-enhanced MRI (DCE-MRI) of ameloblastomas. MRI and DCE-MRI were performed for 10 ameloblastomas. We obtained the following results from the MRI and DCE-MRI. (a) Ameloblastomas can be divided into solid and cystic portions on the basis of MR signal intensities. (b) Ameloblastomas show a predilection for intermediate signal intensity on T1WI, high signal intensity on T2WI, and well enhancement in the solid portion; they also show a homogeneous intermediate signal intensity on T1WI and homogeneous high signal intensity on T2WI, and no enhancement in the cystic portion. (c) The mural nodule or thick wall can be detected in ameloblastomas lesions. (d) CI curves of ameloblastomas show two patterns: the first pattern increases, reaches a plateau at 100-300 s, then sustains the plateau or decreases gradually to 600-900 s, while the other increases relatively rapidly, reaches a plateau at 90-120 s, then decreases relatively rapidly to 300 s, and decreases gradually thereafter. There was no difference in the CI curve patterns among primary and recurrent cases, a case with glandular odontogenic tumor in ameloblastoma or among histopathological types such as plexiform, follicular, mixed, desmoplastic, and unicystic type.

DOI： 10.1016/j.ejrad.2005.01.006

左側下顎切痕部に骨欠損を認めた神経線維腫症1型の1例

久富美紀, 此内浩信, 河井紀子, 本多康聡, 柳文修, 松崎秀信, 村上純, 浅海淳一, 岸幹二

日本口腔診断学会雑誌 18 ( 2 ) 400 - 400 2005年10月

Effects of histone deacetylase inhibitor FR901228 on the expression level of telomerase reverse transcriptase in oral cancer. 国際誌

Jun Murakami, Jun-ichi Asaumi, Noriko Kawai, Hidetsugu Tsujigiwa, Yoshinobu Yanagi, Hitoshi Nagatsuka, Tetsuyoshi Inoue, Susumu Kokeguchi, Shoji Kawasaki, Masahiro Kuroda, Noriaki Tanaka, Nagahide Matsubara, Kanji Kishi

Cancer chemotherapy and pharmacology 56 ( 1 ) 22 - 8 2005年7月

記述言語：英語出版者・発行元：SPRINGER

We speculated whether or not the expression level of telomerase reverse transcriptase (hTERT) would be modulated by agents targeting epigenetics in oral cancer cell lines. Although hTERT is known to be targeted by epigenetic changes, it remains unclear how chemoagents targeting epigenetics work on hTERT transcription. In the present study, the epigenetic effects of the histone deacetylase (HDAC) inhibitor FR901228 on hTERT transcription in oral cancer cell lines were analyzed by RT-PCR. The mRNA expression of hTERT was upregulated after exposure to FR901228 in hTERT-negative Hep2 cells, and even SAS and KB cells expressed high levels of hTERT. Moreover, cotreatment of protein synthesis inhibitor cycloheximide (CHX) resulted in the induction of hTERT transcription by FR901228. This suggests that the induction of hTERT by FR901228 requires de novo protein synthesis to some extent and is more likely a direct than an indirect effect on epigenetic changes such as histone acetylation/deacetylation. We further examined the effect of FR901228 on c-myc protein, which is one of the main hTERT transcription activators. FR901228 repressed c-myc protein only in the absence of CHX, and depended on the enhancement of de novo protein synthesis. Our results indicate that c-myc protein is repressed indirectly by FR901228 but may not contribute to FR901228-induced hTERT transcription. The present study showed that the HDAC inhibitor FR901228 induced the hTERT gene by a complex mechanism that involved transcription factors other than c-myc, in addition to inhibition of histone deacetylation.

DOI： 10.1007/s00280-004-0976-x

メディカルトレンド2005 平成16年度文部科学省科学研究費補助金採択研究課題の成果イメージングによる診断の研究を中心に基盤研究 MRIによる摂食嚥下動態の画像解析法の開発についての研究座位での撮影が可能なMRIを用いた摂食嚥下動態の描出

本多康聡, 石田暸, 柳文修, 河井紀子, 大野誠一郎, 岸幹二

INNERVISION 20 ( 8 ) 48 - 48 2005年6月

記述言語：日本語出版者・発行元：(株)インナービジョン

摂食嚥下動態についてMRIを用いた画像解析法を開発した.今回は,座位での撮影が可能なMRIを用いた摂食嚥下動態の描出について述べた.座位でのMRI撮影は生理的な摂食嚥下動態が観察でき,仰向けの姿勢と比較して安全性が高い.さらに,詳細な動画像の描出のためには時間分解能を改善する必要があった.VFと比較すると,VFは硬組織の描出には優れているが,軟組織の描出は困難で,骨や歯の像と重なるため,舌の描出も明瞭ではなかった.MRIは硬組織の描出ではVFに劣るが,軟組織の描出はきわめて優れていた.また,VFが透視画であるのに対し,MRIは断層像であるため,他組織との重なりがない

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Assessment of lesions arising or extending in the submental region with magnetic resonance imaging

Jun-Ichi Asaumi, Yoshinobu Yanagi, Miki Hisatomi, Hironobu Konouchi, Hidenobu Matsuzaki, Kanji Kishi

Oral Radiology 21 ( 1 ) 6 - 13 2005年6月

記述言語：英語

Objectives. Various types of lesion have been reported in the submental region, but submental masses can be difficult to diagnose. Our objective was to attempt to diagnose submental masses by using magnetic resonance imaging (MRI) along with clinical data. Methods. We attempted to diagnose seven cases in which the chief complaint was submental swelling
diagnosis was carried out on the basis of features visualized by MRI. Results. Seven types of lesions were observed: phlegmone and abscess caused by osteomyelitis
epidermoid cyst
thyroglossal duct cyst
abscess-forming granulomatous lymphadenitis
T-cell lymphoma (suspected Hodgkin's disease)
non-Hodgkin's B-cell lymphoma
and lymph node metastasis from squamous cell carcinoma in the tongue. Conclusions. The magnetic resonance images of each lesion revealed characteristic features. Thus, MRI may be useful for the differential diagnosis of submental swelling before the initiation of treatment. © Japanese Society for Oral and Maxillofacial Radiology Springer-Verlag Tokyo 2005.

DOI： 10.1007/s11282-004-0021-0

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オトガイ部に生じたMicrocystic adnexal carcinomaの一例

柳文修, 村上純, 浅海淳一, 此内浩信, 岸幹二

歯科放射線 45 ( 1 ) 24 - 25 2005年3月

座位での撮影が可能なMRIを用いた摂食嚥下動態の描出

本多康聡, 柳文修, 岸幹二

日本画像医学雑誌 23 ( 4 ) 39 - 39 2005年1月

MRIを用いた摂食嚥下動態の解析法の開発(3) 座位での撮影が可能なMRIを用いた摂食嚥下動態の観察

本多康聡, 柳文修, 河井紀子, 岸幹二, 江草正彦, 石田僚, 森貴幸, 有岡享子

日本摂食・嚥下リハビリテーション学会雑誌 8 ( 2 ) 267 - 268 2004年12月

5フルオロウラシル感受性におけるO6-メチルグアニン-DNAメチルトランスフェラーゼの腫瘍内活性の影響

牧優, 浅海淳一, 柳文修, 村上純, 河井紀子, 本多康聡, 岸幹二

歯科放射線 44 ( 4 ) 269 - 270 2004年12月

座位での撮影が可能なMRIを用いた摂食嚥下動態の描出

本多康聡, 柳文修, 河井紀子, 岸幹二

歯科放射線 44 ( 4 ) 282 - 282 2004年12月

骨移植部を中心に再発をきたしたエナメル上皮腫の2例

崔容碩, 此内浩信, 柳文修, 久富美紀, 松崎秀信, 岸幹二

歯科放射線 44 ( 4 ) 260 - 261 2004年12月

MRIおよびdynamic MRIによるエナメル上皮腫の評価

浅海淳一, 久冨美紀, 柳文修, 比内浩信, 松崎秀信, 河井紀子, 村上純, 牧優, 岸幹二

日本口腔診断学会雑誌 17 ( 2 ) 303 - 303 2004年10月

軟口蓋部腫瘍の2症例のMRIによる診断

此内浩信, 浅海淳一, 久富美紀, 柳文修, 松崎秀信, 河井紀子, 村上純, 本多康聡, 牧優, 岸幹二

日本口腔診断学会雑誌 17 ( 2 ) 303 - 303 2004年10月

Assessment of MRI and dynamic contrast-enhanced MRI in the differential diagnosis of adenomatoid odontogenic tumor. 国際誌

Jun-ichi Asaumi, Yoshinobu Yanagi, Hironobu Konouchi, Miki Hisatomi, Hidenobu Matsuzaki, Hiroshi Shigehara, Kanji Kishi

European journal of radiology 51 ( 3 ) 252 - 6 2004年9月

記述言語：英語出版者・発行元：ELSEVIER SCI IRELAND LTD

The radiographical differentiation of adenomatoid odontogenic tumor (AOT) from dentigerous cysts, calcifying odontogenic cysts, calcifying epithelial odontogenic tumors, odontogenic keratocysts and amelobastomas is sometimes difficult. We attempted to differentiate AOT from other lesions similar to AOT in radiographic findings using MRI. The MRI features of AOT in our three cases included homogeneous low SI in the cystic portion and homogeneous intermediate SI in the solid portion on T1WI, homogeneous high SI in the cystic portion and intermediate to slightly high SI in the solid portion on T2WI and enhancement of only the solid portion on CE-T1WI although none of the sequences included SI of calcifications. The contrast index curves in the three cases of AOT showed a gradual increase to 300 s, which signified a benign tumor. These MRI features were characteristic features of AOT and might be a basis for differentiating AOT from the above possible lesions in radiographic examinations.

DOI： 10.1016/j.ejrad.2003.09.009

Web of Science

PubMed

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粘液腫のMRI所見について

柳文修

歯科放射線 44 ( 3 ) 191 - 192 2004年9月

下顎骨転移性腫瘍3例のX線学的検討

河井紀子, 浅海淳一, 柳文修, 松崎秀信, 岸幹二

歯科放射線 44 ( 3 ) 194 - 194 2004年9月

悪性リンパ腫を疑った顎下部の反応性リンパ様過形成の一例

柳文修, 浅海淳一, 河井紀子, 此内浩信, 岸幹二

歯科放射線 44 ( 3 ) 189 - 189 2004年9月

Effects of demethylating agent 5-aza-2(')-deoxycytidine and histone deacetylase inhibitor FR901228 on maspin gene expression in oral cancer cell lines. 国際誌

Jun Murakami, Jun-Ichi Asaumi, Yuu Maki, Hidetsugu Tsujigiwa, Masahiro Kuroda, Noriyuki Nagai, Yoshinobu Yanagi, Tetsuyoshi Inoue, Shoji Kawasaki, Noriaki Tanaka, Nagahide Matsubara, Kanji Kishi

Oral oncology 40 ( 6 ) 597 - 603 2004年7月

記述言語：英語出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

Maspin, which belongs to the serine protease inhibitor (serpin) superfamily, has been proposed as a potent tumor suppressor that inhibits cell motility, invasion, angiogenesis, and metastasis. In the present study, we examined the effects of 5-aza-2(')-deoxycytidine (5-aza-dC), a demethylating agent, and FR901228, a histone deacetylase (HDAC) inhibitor, on maspin expression in oral cancer cell lines. The expression levels of maspin mRNA were divided into two groups, which was the maspin low-expressed and high-expressed cell lines in the 12 oral cancer cell lines. The maspin promoter contained only a few methylated CpG sites in the maspin low-expressed cell lines. Moreover, the methylation status was not altered after 5-aza-dC treatment. However, the transcription of the maspin gene was clearly increased following 5-aza-dC treatment in a number of oral cancer cell lines. These results imply that an action of 5-aza-dC is separate from induction of promoter demethylation. Treatment with FR901228 resulted in a time-dependent stimulation of the re-expression of maspin mRNA as early as 4 h after treatment in the maspin downregulated cells. The re-expression of the maspin gene may contribute to the recuperation of biological functions linked to FR901228 such as an inhibitory effect on tumor angiogenesis and cell invasion. These results indicate that maspin and its target genes may be excellent leads for future studies on the potential benefits of FR901228, a HDAC inhibitor, in cancer therapy.

DOI： 10.1016/j.oraloncology.2003.12.008

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