研究者詳細 - 近藤　秀樹

論文 - 近藤　秀樹

分割表示 144 件中 41 - 60 件目／全件表示 >>

A Transfectable Fusagravirus from a Japanese Strain of Cryphonectria carpinicola with Spherical Particles. 査読国際誌

Subha Das, Sakae Hisano, Ana Eusebio-Cope, Hideki Kondo, Nobuhiro Suzuki

Viruses 14 ( 8 ) 2022年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

A novel dsRNA virus (Cryphonectria carpinicola fusagravirus 1, CcFGV1), isolated from a Japanese strain (JS13) of Cryphonectria carpinicola, was thoroughly characterized. The biological comparison of a set of isogenic CcFGV1-infected and -free (JS13VF) strains indicated asymptomatic infection by CcFGV1. The sequence analysis showed that the virus has a two open reading frame (ORF) genome of 9.6 kbp with the RNA-directed RNA polymerase domain encoded by ORF2. The N-terminal sequencing and peptide mass fingerprinting showed an N-terminally processed or degraded product (150 kDa) of the 5'-proximal ORF1-encoded protein (1462 amino acids) to make up the CcFGV1 spherical particles of ~40 nm in diameter. Interestingly, a portion of CcFGV1 dsRNA co-fractionated with a host protein of 70 kDa. The purified CcFGV1 particles were used to transfect protoplasts of JS13VF as well as the standard strain of an experimental model filamentous fungal host Cryphonectria parasitica. CcFGV1 was confirmed to be associated with asymptomatic infection of both fungi. RNA silencing was shown to target the virus in C. parasitica, resulting in reduced CcFGV1 accumulation by comparing the CcFGV1 content between RNA silencing-competent and -deficient strains. These results indicate the transfectability of spherical particles of a fusagravirus associated with asymptomatic infection.

DOI： 10.3390/v14081722

Mycovirus Hunting Revealed the Presence of Diverse Viruses in a Single Isolate of the Phytopathogenic Fungus Diplodia seriata From Pakistan 査読

Haris Ahmed Khan, Paul Telengech, Hideki Kondo, Muhammad Faraz Bhatti, Nobuhiro Suzuki

Frontiers in Cellular and Infection Microbiology 12 2022年6月

掲載種別：研究論文（学術雑誌）出版者・発行元：Frontiers Media SA

Diplodia seriata in the family Botryosphaeriaceae is a cosmopolitan phytopathogenic fungus and is responsible for causing cankers, fruit rot and leaf spots on economically important plants. In this study, we characterized the virome of a single Pakistani strain (L3) of D. seriata. Several viral-like contig sequences were obtained via a previously conducted next-generation sequencing analysis. Multiple infection of the L3 strain by eight RNA mycoviruses was confirmed through RT-PCR using total RNA samples extracted from this strain; the entire genomes were determined via Sanger sequencing of RT-PCR and RACE clones. A BLAST search and phylogenetic analyses indicated that these eight mycoviruses belong to seven different viral families. Four identified mycoviruses belong to double-stranded RNA viral families, including Polymycoviridae, Chrysoviridae, Totiviridae and Partitiviridae, and the remaining four identified mycoviruses belong to single-stranded RNA viral families, i.e., Botourmiaviridae, and two previously proposed families “Ambiguiviridae” and “Splipalmiviridae”. Of the eight, five mycoviruses appear to represent new virus species. A morphological comparison of L3 and partially cured strain L3ht1 suggested that one or more of the three viruses belonging to Polymycoviridae, “Splipalmiviridae” and “Ambiguiviridae” are involved in the irregular colony phenotype of L3. To our knowledge, this is the first report of diverse virome characterization from D. seriata.

DOI： 10.3389/fcimb.2022.913619

Coat protein of Chinese wheat mosaic virus upregulates and interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase, a negative regulator of plant autophagy, to promote virus infection. 査読国際誌

Erbo Niu, Chaozheng Ye, Wanying Zhao, Hideki Kondo, Yunfeng Wu, Jianping Chen, Ida Bagus Andika, Liying Sun

Journal of integrative plant biology 2022年6月

記述言語：英語掲載種別：研究論文（学術雑誌）

Autophagy is an intracellular degradation mechanism involved in antiviral defense, but the strategies employed by plant viruses to counteract autophagy-related defense remain unknown for the majority of the viruses. Herein, we describe how the Chinese wheat mosaic virus (CWMV, genus Furovirus) interferes with autophagy and enhances its infection in Nicotiana benthamiana. Yeast two-hybrid screening and in vivo/in vitro assays revealed that the 19 kDa coat protein (CP19K) of CWMV interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs), negative regulators of autophagy, which bind autophagy-related protein 3 (ATG3), a key factor in autophagy. CP19K also directly interacts with ATG3, possibly leading to the formation of a CP19K-GAPC-ATG3 complex. CP19K-GAPC interaction appeared to intensify CP19K-ATG3 binding. Moreover, CP19K expression upregulated GAPC gene transcripts and reduced autophagic activities. Accordingly, the silencing of GAPC genes in transgenic N. benthamiana reduced CWMV accumulation, whereas CP19K overexpression enhanced it. Overall, our results suggest that CWMV CP19K interferes with autophagy through the promotion and utilization of the GAPC role as a negative regulator of autophagy. This article is protected by copyright. All rights reserved.

DOI： 10.1111/jipb.13313

ICTV Virus Taxonomy Profile: Rhabdoviridae 2022. 査読国際誌

Peter J Walker, Juliana Freitas-Astúa, Nicolas Bejerman, Kim R Blasdell, Rachel Breyta, Ralf G Dietzgen, Anthony R Fooks, Hideki Kondo, Gael Kurath, Ivan V Kuzmin, Pedro Luis Ramos-González, Mang Shi, David M Stone, Robert B Tesh, Noël Tordo, Nikos Vasilakis, Anna E Whitfield, Ictv Report Consortium

The Journal of general virology 103 ( 6 ) 2022年6月

記述言語：英語掲載種別：研究論文（学術雑誌）

The family Rhabdoviridae comprises viruses with negative-sense (-) RNA genomes of 10-16 kb. Virions are typically enveloped with bullet-shaped or bacilliform morphology but can also be non-enveloped filaments. Rhabdoviruses infect plants or animals, including mammals, birds, reptiles, amphibians or fish, as well as arthropods, which serve as single hosts or act as biological vectors for transmission to animals or plants. Rhabdoviruses include important pathogens of humans, livestock, fish or agricultural crops. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Rhabdoviridae, which is available at ictv.global/report/rhabdoviridae.

DOI： 10.1099/jgv.0.001689

A novel deltapartitivirus from red clover. 査読国際誌

Paul Telengech, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

Archives of virology 167 ( 4 ) 1201 - 1204 2022年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

The family Partitiviridae has five genera, among which is the genus Deltapartitivirus. We report here the complete genome sequence of a deltapartitivirus from red clover, termed "red clover cryptic virus 3" (RCCV3). RCCV3 has a bisegmented double-stranded (ds) RNA genome. dsRNA1 and dsRNA2 are 1580 and 1589 nucleotides (nt) in length and are predicted to encode an RNA-directed RNA polymerase (RdRP) and a capsid protein (CP), respectively. The RCCV3 RdRP shares the highest sequence identity with the RdRP of a previously reported deltapartitivirus, Medicago sativa deltapartitivirus 1 (MsDPV1) (76.5%), while the RCCV3 CP shows 50% sequence identity to the CP of MsDPV1. RdRP- and CP-based phylogenetic trees place RCCV3 into a clade of deltapartitiviruses. The sequence and phylogenetic analyses clearly indicate that RCCV3 represents a new species in the genus Deltapartitivirus. RCCV3 was detectable in all three tested cultivars of red clover.

DOI： 10.1007/s00705-022-05372-3

A novel victorivirus from the phytopathogenic fungus Neofusicoccum parvum. 査読国際誌

Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

Archives of virology 167 ( 3 ) 923 - 929 2022年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

Neofusicoccum parvum is an important plant-pathogenic ascomycetous fungus that causes trunk diseases in a variety of plants. A limited number of reports on mycoviruses from this fungus are available. Here, we report the characterization of a novel victorivirus, Neofusicoccum parvum victorivirus 3 (NpVV3). An agarose gel dsRNA profile of a Pakistani strain of N. parvum, NFN, showed a band of ~5 kbp that was not detectable in Japanese strains of N. parvum. Taking a high-throughput and Sanger sequencing approach, the complete genome sequence of NpVV3 was determined to be 5226 bp in length with two open reading frames (ORF1 and ORF2) that encode a capsid protein (CP) and an RNA-dependent RNA polymerase (RdRP). The RdRP appears to be translated by a stop/restart mechanism facilitated by the junction sequence AUGucUGA, as is found in some other victoriviruses. BLASTp searches showed that NpVV3 CP and RdRP share the highest amino acid sequence identity (80.5% and 72.4%, respectively) with the corresponding proteins of NpVV1 isolated from a French strain of N. parvum. However, NpVV3 was found to be different from NpVV1 in its terminal sequences and the stop/restart facilitator sequence. NpVV3 particles ~35 nm in diameter were partially purified and used to infect an antiviral-RNA-silencing-deficient strain (∆dcl2) of an experimental ascomycetous fungal host, Cryphonectria parasitica. NpVV3 showed symptomless infection in the new host strain.

DOI： 10.1007/s00705-021-05304-7

Plant viruses and viroids in Japan 査読

Shin ichi Fuji, Tomofumi Mochizuki, Mitsuru Okuda, Shinya Tsuda, Satoshi Kagiwada, Ken Taro Sekine, Masashi Ugaki, Keiko T. Natsuaki, Masamichi Isogai, Tetsuo Maoka, Minoru Takeshita, Nobuyuki Yoshikawa, Kazuyuki Mise, Takahide Sasaya, Hideki Kondo, Kenji Kubota, Yasuyuki Yamaji, Toru Iwanami, Kazusato Ohshima, Kappei Kobayashi, Tatsuji Hataya, Teruo Sano, Nobuhiro Suzuki

Journal of General Plant Pathology 88 ( 2 ) 105 - 127 2022年3月

An increasing number of plant viruses and viroids have been reported from all over the world due largely to metavirogenomics approaches with technological innovation. Herein, the official changes of virus taxonomy, including the establishment of megataxonomy and amendments of the codes of virus classification and nomenclature, recently made by the International Committee on Taxonomy of Viruses were summarized. The continued efforts of the plant virology community of Japan to index all plant viruses and viroids occurring in Japan, which represent 407 viruses, including 303 virus species and 104 unclassified viruses, and 25 viroids, including 20 species and 5 unclassified viroids, as of October 2021, were also introduced. These viruses and viroids are collectively classified into 81 genera within 26 families of 3 kingdoms (Shotokuvirae, Orthornavirae, Pararnavirae) across 2 realms (Monodnaviria and Riboviria). This review also overviewed how Japan’s plant virus/viroid studies have contributed to advance virus/viroid taxonomy.

DOI： 10.1007/s10327-022-01051-y

A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe. 査読国際誌

Yukiyo Sato, Sabitree Shahi, Paul Telengech, Sakae Hisano, Carolina Cornejo, Daniel Rigling, Hideki Kondo, Nobuhiro Suzuki

Virus research 307 198606 - 198606 2022年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Positive-sense (+), single-stranded (ss) RNA viruses with divided RNA-dependent RNA polymerase (RdRP) domains have been reported from diverse filamentous ascomycetes since 2020. These viruses are termed splipalmiviruses or polynarnaviruses and have been characterized largely at the sequence level, but ill-defined biologically. Cryphonectria naterciae, from which only one virus has been reported, is an ascomycetous fungus potentially plant-pathogenic to chestnut and oak trees. We molecularly characterized multiple viruses in a single Portuguese isolate (C0614) of C. naterciae, taking a metatranscriptomic and conventional double-stranded RNA approach. Among them are a novel splipalmivirus (Cryphonectria naterciae splipalmivirus 1, CnSpV1) and a novel fusagravirus (Cryphonectria naterciae fusagravirus 1, CnFGV1). This study focused on the former virus. CnSpV1 has a tetra-segmented, (+)ssRNA genome (RNA1 to RNA4). As observed for other splipalmiviruses reported in 2020 and 2021, the RNA-dependent RNA polymerase domain is separately encoded by RNA1 (motifs F, A and B) and RNA2 (motifs C and D). A hypothetical protein encoded by the 5'-proximal open reading frame of RNA3 shows similarity to a counterpart conserved in some splipalmiviruses. The other RNA3-encoded protein and RNA4-encoded protein show no similarity with known proteins in a blastp search. The tetra-segment nature was confirmed by the conserved terminal sequences of the four CnSpV1 segments (RNA1 to RNA4) and their 100% coexistence in over 100 single conidial isolates tested. The experimental introduction of CnSpV1 along with CnFGV1 into a virus free strain C0754 of C. naterciae vegetatively incompatible with C0614 resulted in no phenotypic alteration, suggesting asymptomatic infection. The protoplast fusion assay indicates a considerably narrow host range of CnSpV1, restricted to the species C. naterciae and C. carpinicola. This study contributes to better understanding of the molecular and biological properties of this unique group of viruses.

DOI： 10.1016/j.virusres.2021.198606

Distinctive in vitro ATP Hydrolysis Activity of AtVIPP1, a Chloroplastic ESCRT-III Superfamily Protein in Arabidopsis. 査読国際誌

Norikazu Ohnishi, Manabu Sugimoto, Hideki Kondo, Ken-Ichi Shioya, Lingang Zhang, Wataru Sakamoto

Frontiers in plant science 13 949578 - 949578 2022年

記述言語：英語掲載種別：研究論文（学術雑誌）

Vesicle-inducing protein in plastid 1 (VIPP1), characteristic to oxygenic photosynthetic organisms, is a membrane-remodeling factor that forms homo-oligomers and functions in thylakoid membrane formation and maintenance. The cyanobacterial VIPP1 structure revealed a monomeric folding pattern similar to that of endosomal sorting complex required for transport (ESCRT) III. Characteristic to VIPP1, however, is its own GTP and ATP hydrolytic activity without canonical domains. In this study, we found that histidine-tagged Arabidopsis VIPP1 (AtVIPP1) hydrolyzed GTP and ATP to produce GDP and ADP in vitro, respectively. Unexpectedly, the observed GTPase and ATPase activities were biochemically distinguishable, because the ATPase was optimized for alkaline conditions and dependent on Ca2+ as well as Mg2+, with a higher affinity for ATP than GTP. We found that a version of AtVIPP1 protein with a mutation in its nucleotide-binding site, as deduced from the cyanobacterial structure, retained its hydrolytic activity, suggesting that Arabidopsis and cyanobacterial VIPP1s have different properties. Negative staining particle analysis showed that AtVIPP1 formed particle or rod structures that differed from those of cyanobacteria and Chlamydomonas. These results suggested that the nucleotide hydrolytic activity and oligomer formation of VIPP1 are common in photosynthetic organisms, whereas their properties differ among species.

DOI： 10.3389/fpls.2022.949578

2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. 査読国際誌

Jens H Kuhn, Scott Adkins, Bernard R Agwanda, Rim Al Kubrusli, Sergey V Alkhovsky, Gaya K Amarasinghe, Tatjana Avšič-Županc, María A Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J Ballinger, Christopher F Basler, Sina Bavari, Martin Beer, Nicolas Bejerman, Andrew J Bennett, Dennis A Bente, Éric Bergeron, Brian H Bird, Carol D Blair, Kim R Blasdell, Dag-Ragnar Blystad, Jamie Bojko, Wayne B Borth, Steven Bradfute, Rachel Breyta, Thomas Briese, Paul A Brown, Judith K Brown, Ursula J Buchholz, Michael J Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Büttner, Charles H Calisher, Mengji Cao, Inmaculada Casas, Kartik Chandran, Rémi N Charrel, Qi Cheng, Yuya Chiaki, Marco Chiapello, Il-Ryong Choi, Marina Ciuffo, J Christopher S Clegg, Ian Crozier, Elena Dal Bó, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L de Swart, Humberto Debat, Nolwenn M Dheilly, Emiliano Di Cicco, Nicholas Di Paola, Francesco Di Serio, Ralf G Dietzgen, Michele Digiaro, Olga Dolnik, Michael A Drebot, J Felix Drexler, William G Dundon, W Paul Duprex, Ralf Dürrwald, John M Dye, Andrew J Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Hugh W Ferguson, Anthony R Fooks, Marco Forgia, Pierre B H Formenty, Jana Fránová, Juliana Freitas-Astúa, Jingjing Fu, Stephanie Fürl, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R Garrison, Thomas Gaskin, Jean-Paul J Gonzalez, Anthony Griffiths, Tony L Goldberg, Martin H Groschup, Stephan Günther, Roy A Hall, John Hammond, Tong Han, Jussi Hepojoki, Roger Hewson, Jiang Hong, Ni Hong, Seiji Hongo, Masayuki Horie, John S Hu, Tao Hu, Holly R Hughes, Florian Hüttner, Timothy H Hyndman, M Ilyas, Risto Jalkanen, Dàohóng Jiāng, Gilda B Jonson, Sandra Junglen, Fujio Kadono, Karia H Kaukinen, Michael Kawate, Boris Klempa, Jonas Klingström, Gary Kobinger, Igor Koloniuk, Hideki Kondō, Eugene V Koonin, Mart Krupovic, Kenji Kubota, Gael Kurath, Lies Laenen, Amy J Lambert, Stanley L Langevin, Benhur Lee, Elliot J Lefkowitz, Eric M Leroy, Shaorong Li, Longhui Li, Jiànróng Lǐ, Huazhen Liu, Igor S Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H Marshall, Shin-Yi L Marzano, Sebastien Massart, John W McCauley, Michael Melzer, Nicole Mielke-Ehret, Kristina M Miller, Tobi J Ming, Ali Mirazimi, Gideon J Mordecai, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, José A Navarro, Sergey V Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R T Nunes, Alejandro Olmedo-Velarde, Gustavo Palacios, Vicente Pallás, Bernadett Pályi, Anna Papa, Sofia Paraskevopoulou, Adam C Park, Colin R Parrish, David A Patterson, Alex Pauvolid-Corrêa, Janusz T Pawęska, Susan Payne, Carlotta Peracchio, Daniel R Pérez, Thomas S Postler, Liying Qi, Sheli R Radoshitzky, Renato O Resende, Carina A Reyes, Bertus K Rima, Gabriel Robles Luna, Víctor Romanowski, Paul Rota, Dennis Rubbenstroth, Luisa Rubino, Jonathan A Runstadler, Sead Sabanadzovic, Amadou Alpha Sall, Maria S Salvato, Rosemary Sang, Takahide Sasaya, Angela D Schulze, Martin Schwemmle, Mang Shi, Xiǎohóng Shí, Zhènglì Shí, Yoshifumi Shimomoto, Yukio Shirako, Stuart G Siddell, Peter Simmonds, Manuela Sironi, Guy Smagghe, Sophie Smither, Jin-Won Song, Kirsten Spann, Jessica R Spengler, Mark D Stenglein, David M Stone, Jari Sugano, Curtis A Suttle, Amy Tabata, Ayato Takada, Shigeharu Takeuchi, David P Tchouassi, Amy Teffer, Robert B Tesh, Natalie J Thornburg, Yasuhiro Tomitaka, Keizō Tomonaga, Noël Tordo, Baldwyn Torto, Jonathan S Towner, Shinya Tsuda, Changchun Tu, Massimo Turina, Ioannis E Tzanetakis, Janice Uchida, Tomio Usugi, Anna Maria Vaira, Marta Vallino, Bernadette van den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J Walker, Lin-Fa Wang, Guoping Wang, Yanxiang Wang, Yaqin Wang, Muhammad Waqas, Tàiyún Wèi, Shaohua Wen, Anna E Whitfield, John V Williams, Yuri I Wolf, Jiangxiang Wu, Lei Xu, Hironobu Yanagisawa, Caixia Yang, Zuokun Yang, F Murilo Zerbini, Lifeng Zhai, Yong-Zhen Zhang, Song Zhang, Jinguo Zhang, Zhe Zhang, Xueping Zhou

Archives of virology 166 ( 12 ) 3513 - 3566 2021年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.

DOI： 10.1007/s00705-021-05143-6

Assessment of mycoviral diversity in Pakistani fungal isolates revealed infection by 11 novel viruses of a single strain of Fusarium mangiferae isolate SP1. 査読国際誌

Haris Ahmed Khan, Wajeeha Shamsi, Atif Jamal, Memoona Javaied, Mashal Sadiq, Tehsin Fatma, Aqeel Ahmed, Maleeha Arshad, Mubashra Waseem, Samra Babar, Midhat Mustafa Dogar, Nasar Virk, Hussnain Ahmed Janjua, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

The Journal of general virology 102 ( 12 ) 2021年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

An extensive screening survey was conducted on Pakistani filamentous fungal isolates for the identification of viral infections. A total of 396 fungal samples were screened, of which 36 isolates were found double-stranded (ds) RNA positive with an overall frequency of 9% when analysed by a classical dsRNA isolation method. One of 36 dsRNA-positive strains, strain SP1 of a plant pathogenic fungus Fusarium mangiferae, was subjected to virome analysis. Next-generation sequencing and subsequent completion of the entire genome sequencing by a classical Sanger sequencing method showed the SP1 strain to be co-infected by 11 distinct viruses, at least seven of which should be described as new taxa at the species level according to the ICTV (International Committee on Taxonomy of Viruses) species demarcation criteria. The newly identified F. mangiferae viruses (FmVs) include two partitivirids, one betapartitivirus (FmPV1) and one gammapartitivirus (FmPV2); six mitovirids, three unuamitovirus (FmMV2, FmMV4, FmMV6), one duamitovirus (FmMV5), and two unclassified mitovirids (FmMV1, FmMV3); and three botourmiavirids, two magoulivirus (FmBOV1, FmBOV3) and one scleroulivirus (FmBOV2). The number of coinfecting viruses is among the largest ones of fungal coinfections. Their molecular features are thoroughly described here. This represents the first large virus survey in the Indian sub-continent.

DOI： 10.1099/jgv.0.001690

Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand. 査読

Sokty Neang, Santiti Bincader, Sansern Rangsuwan, Pisut Keawmanee, Soriya Rin, Lakha Salaipeth, Subha Das, Hideki Kondo, Nobuhiro Suzuki, Ikuo Sato, Daigo Takemoto, Chainarong Rattanakreetakul, Ratiya Pongpisutta, Masao Arakawa, Sotaro Chiba

Viruses 13 ( 11 ) 2269 2021年11月

掲載種別：研究論文（学術雑誌）

Partitiviruses are one of the most prevalent double-stranded RNA viruses that have been identified mostly in filamentous fungi and plants. Partitiviruses generally infect host fungi asymptomatically but infrequently exert significant effect(s) on morphology and virulence, thus being considered a potential source of biological control agents against pathogenic fungi. In this study, we performed a screening for mycoviruses of a collection of Thai isolates of rice fungal pathogen Rhizoctonia oryzae-sativae, a causal agent of rice aggregated sheath spot disease. As a result, 36% of tested isolates carried potentially viral double-stranded RNAs with sizes ranging from 2 to 3 kbp. By conventional cDNA library construction and RNA-seq, we determined six new alphapartitiviruses that infected three isolates: tentatively named Rhizoctonia oryzae-sativae partitivirus 1 to 6 (RosPV1-6). Furthermore, RT-PCR detection of each virus revealed their omnipresent nature in different R. oryzaesativae isolates. Although virus-curing of basidiomycetous fungi is generally difficult, our repeated attempts successfully obtained virus-free (for RosPV1, RosPV2, and uncharacterized partitiviruses), isogenic strain of R. oryzae-sativae TSS190442. The virus-cured strain showed slightly faster colony growth on the synthetic media and severe symptom development on the rice sheath compared to its virus-infected counterpart. Overall, this study shed light on the distribution of partitiviruses in R. oryzae-sativae in a paddy environment and exemplified a virus-curing protocol that may be applicable for other basidiomycetous fungi.

DOI： 10.3390/v13112269

ICTV Virus Taxonomy Profile: Nyamiviridae 2021. 査読国際誌

Ralf G Dietzgen, Andrew E Firth, Dàohóng Jiāng, Sandra Junglen, Hideki Kondo, Jens H Kuhn, Sofia Paraskevopoulou, Nikos Vasilakis, Ictv Report Consortium

The Journal of general virology 102 ( 11 ) 2021年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Nyamiviridae is a family of viruses in the order Mononegavirales, with unsegmented (except for members of the genus Tapwovirus), negative-sense RNA genomes of 10-13 kb. Nyamviruses have a genome organisation and content similar to that of other mononegaviruses. Nyamiviridae includes several genera that form monophyletic clades on phylogenetic analysis of the RNA polymerase. Nyamiviruses have been found associated with diverse invertebrates as well as land- and seabirds. Members of the genera Nyavirus and Socyvirus produce enveloped, spherical virions. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Nyamiviridae, which is available at ictv.global/report/nyamiviridae.

DOI： 10.1099/jgv.0.001681

Epidemic progress of beet necrotic yellow vein virus: Evidence from an investigation in Japan spanning half a century 査読

Ryo Nakagami, Sotaro Chiba, Naoto Yoshida, Yoshiteru Senoo, Minako Iketani-Saito, Satoru Iketani, Hideki Kondo, Tetsuo Tamada

PLANT PATHOLOGY 2021年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY

Beet necrotic yellow vein virus (BNYVV) is the causal agent of rhizomania, the most serious sugar beet disease worldwide. Since the first finding in Japan in 1969, BNYVV became widespread throughout Hokkaido in a few decades and led to the introduction of Rz1-resistant sugar beet cultivars in the 1990s. Here, we report the historical progress of the BNYVV epidemic in Hokkaido from 1969 to 2019. Previous analysis on samples from 1991 showed that BNYVV isolates were classified into three strains (named O, D, and T) based on the RNA3-encoded p25 gene. The O-type viruses were widely detected in Hokkaido, while the D- and T-type viruses were detected in limited areas. The RNA5, encoding the p26 gene, was initially contained in some D- and O-type isolates but not in any T-type isolates. Interestingly, recent sample analysis revealed that RNA5-containing T-type viruses, seemingly more virulent than the other two strains, were widely detected in Hokkaido. Additionally, a small group of virus isolates harbouring a new p25 gene (named C) was found in limited areas. These results suggest that the T-type viruses, which accompanied RNA5, have been preferentially spread from a limited area to other districts over the last few decades and that this spread might be strongly associated with the recent introduction of Rz1-resistant sugar beet cultivars. BNYVV-positive samples also contained mainly beet soil-borne virus and traces of beet virus Q, both of which are the first to be recorded in Japan.

DOI： 10.1111/ppa.13504

A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai. 国際誌

Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

Archives of virology 166 ( 10 ) 2711 - 2722 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungus Fusarium oxysporum. Here, we describe the molecular and biological characterisation of a second hadakavirus strain from Fusarium nygamai, which has not been investigated in detail previously as a virus host. This virus, hadakavirus 1 strain 1NL (HadV1-1NL), has features similar to the first hadakavirus, HadV1-7n, despite having a different number of segments (10 for HadV1-1NL vs. 11 for HadV1-7n). The 10 genomic RNA segments of HadV1-1NL range in size from 0.9 kb to 2.5 kb. All HadV1-1NL segments show 67% to 86% local nucleotide sequence identity to their HadV1-7n counterparts, whereas HadV1-1NL has no homolog of HadV1-7n RNA8, which encodes a zinc-finger motif. Another interesting feature is the possible coding incapability of HadV1-1NL RNA10. HadV1-1NL was predicted to be capsidless based on the RNase A susceptibility of its replicative form dsRNA. Phenotypic comparison of multiple virus-infected and virus-free single-spore isolates indicated asymptomatic infection by HadV1-1NL. Less-efficient vertical transmission via spores was observed as the infected fungal colonies from which the spores were derived became older, as was observed for HadV1-7n. This study shows a second example of a hadakavirus that appears to have unusual features.

DOI： 10.1007/s00705-021-05176-x

Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range. 国際誌

Sabitree Shahi, Sotaro Chiba, Hideki Kondo, Nobuhiro Suzuki

Virology 554 55 - 65 2021年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Cryphonectria nitschkei chrysovirus 1 (CnCV1), was described earlier from an ascomycetous fungus, Cryphonectria nitschkei strain OB5/11, collected in Japan; its partial sequence was reported a decade ago. Complete sequencing of the four genomic dsRNA segments revealed molecular features similar to but distinct from previously reported members of the family Chrysoviridae. Unique features include the presence of a mini-cistron preceding the major large open reading frame in each genomic segment. Common features include the presence of CAA repeats in the 5'-untranslated regions and conserved terminal sequences. CnCV1-OB5/11 could be laterally transferred to C. nitschkei and its relatives C. radicalis and C. naterciae via coculturing, virion transfection and protoplast fusion, but not to fungal species other than the three species mentioned above, even within the genus Cryphonectria, suggesting a very narrow host range. Phenotypic comparison of a few sets of CnCV1-infected and -free isogenic strains showed symptomless infection in new hosts.

DOI： 10.1016/j.virol.2020.11.011

Identification of an RNA Silencing Suppressor Encoded by a Symptomless Fungal Hypovirus, Cryphonectria Hypovirus 4. 国際誌

Annisa Aulia, Kiwamu Hyodo, Sakae Hisano, Hideki Kondo, Bradley I Hillman, Nobuhiro Suzuki

Biology 10 ( 2 ) 2021年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Previously, we have reported the ability of a symptomless hypovirus Cryphonectria hypovirus 4 (CHV4) of the chestnut blight fungus to facilitate stable infection by a co-infecting mycoreovirus 2 (MyRV2)-likely through the inhibitory effect of CHV4 on RNA silencing (Aulia et al., Virology, 2019). In this study, the N-terminal portion of the CHV4 polyprotein, termed p24, is identified as an autocatalytic protease capable of suppressing host antiviral RNA silencing. Using a bacterial expression system, CHV4 p24 is shown to cleave autocatalytically at the di-glycine peptide (Gly214-Gly215) of the polyprotein through its protease activity. Transgenic expression of CHV4 p24 in Cryphonectria parasitica suppresses the induction of one of the key genes of the antiviral RNA silencing, dicer-like 2, and stabilizes the infection of RNA silencing-susceptible virus MyRV2. This study shows functional similarity between CHV4 p24 and its homolog p29, encoded by the symptomatic prototype hypovirus CHV1.

DOI： 10.3390/biology10020100

2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. 国際誌

Jens H Kuhn, Scott Adkins, Daniela Alioto, Sergey V Alkhovsky, Gaya K Amarasinghe, Simon J Anthony, Tatjana Avšič-Županc, María A Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J Ballinger, Tomáš Bartonička, Christopher Basler, Sina Bavari, Martin Beer, Dennis A Bente, Éric Bergeron, Brian H Bird, Carol Blair, Kim R Blasdell, Steven B Bradfute, Rachel Breyta, Thomas Briese, Paul A Brown, Ursula J Buchholz, Michael J Buchmeier, Alexander Bukreyev, Felicity Burt, Nihal Buzkan, Charles H Calisher, Mengji Cao, Inmaculada Casas, John Chamberlain, Kartik Chandran, Rémi N Charrel, Biao Chen, Michela Chiumenti, Il-Ryong Choi, J Christopher S Clegg, Ian Crozier, John V da Graça, Elena Dal Bó, Alberto M R Dávila, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L de Swart, Patrick L Di Bello, Nicholas Di Paola, Francesco Di Serio, Ralf G Dietzgen, Michele Digiaro, Valerian V Dolja, Olga Dolnik, Michael A Drebot, Jan Felix Drexler, Ralf Dürrwald, Lucie Dufkova, William G Dundon, W Paul Duprex, John M Dye, Andrew J Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Jorlan Fernandes, Anthony R Fooks, Pierre B H Formenty, Leonie F Forth, Ron A M Fouchier, Juliana Freitas-Astúa, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R Garrison, Aiah Gbakima, Tracey Goldstein, Jean-Paul J Gonzalez, Anthony Griffiths, Martin H Groschup, Stephan Günther, Alexandro Guterres, Roy A Hall, John Hammond, Mohamed Hassan, Jussi Hepojoki, Satu Hepojoki, Udo Hetzel, Roger Hewson, Bernd Hoffmann, Seiji Hongo, Dirk Höper, Masayuki Horie, Holly R Hughes, Timothy H Hyndman, Amara Jambai, Rodrigo Jardim, Dàohóng Jiāng, Qi Jin, Gilda B Jonson, Sandra Junglen, Serpil Karadağ, Karen E Keller, Boris Klempa, Jonas Klingström, Gary Kobinger, Hideki Kondō, Eugene V Koonin, Mart Krupovic, Gael Kurath, Ivan V Kuzmin, Lies Laenen, Robert A Lamb, Amy J Lambert, Stanley L Langevin, Benhur Lee, Elba R S Lemos, Eric M Leroy, Dexin Li, Jiànróng Lǐ, Mifang Liang, Wénwén Liú, Yàn Liú, Igor S Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H Marshall, Giovanni P Martelli, Robert R Martin, Shin-Yi L Marzano, Sébastien Massart, John W McCauley, Nicole Mielke-Ehret, Angelantonio Minafra, Maria Minutolo, Ali Mirazimi, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, Beatriz Navarro, José A Navarro, Sergey V Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R T Nunes, Are Nylund, Arnfinn L Økland, Renata C Oliveira, Gustavo Palacios, Vicente Pallas, Bernadett Pályi, Anna Papa, Colin R Parrish, Alex Pauvolid-Corrêa, Janusz T Pawęska, Susan Payne, Daniel R Pérez, Florian Pfaff, Sheli R Radoshitzky, Aziz-Ul Rahman, Pedro L Ramos-González, Renato O Resende, Carina A Reyes, Bertus K Rima, Víctor Romanowski, Gabriel Robles Luna, Paul Rota, Dennis Rubbenstroth, Jonathan A Runstadler, Daniel Ruzek, Sead Sabanadzovic, Jiří Salát, Amadou Alpha Sall, Maria S Salvato, Kamil Sarpkaya, Takahide Sasaya, Martin Schwemmle, Muhammad Z Shabbir, Xiǎohóng Shí, Zhènglì Shí, Yukio Shirako, Peter Simmonds, Jana Širmarová, Manuela Sironi, Sophie Smither, Teemu Smura, Jin-Won Song, Kirsten M Spann, Jessica R Spengler, Mark D Stenglein, David M Stone, Petra Straková, Ayato Takada, Robert B Tesh, Natalie J Thornburg, Keizō Tomonaga, Noël Tordo, Jonathan S Towner, Massimo Turina, Ioannis Tzanetakis, Rainer G Ulrich, Anna Maria Vaira, Bernadette van den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Victoria Wahl, Peter J Walker, Hui Wang, Jianwei Wang, Xifeng Wang, Lin-Fa Wang, Tàiyún Wèi, Heather Wells, Anna E Whitfield, John V Williams, Yuri I Wolf, Zhìqiáng Wú, Xin Yang, Xīnglóu Yáng, Xuejie Yu, Natalya Yutin, F Murilo Zerbini, Tong Zhang, Yong-Zhen Zhang, Guohui Zhou, Xueping Zhou

Archives of virology 165 ( 12 ) 3023 - 3072 2020年12月

記述言語：英語

In March 2020, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. At the genus rank, 20 new genera were added, two were deleted, one was moved, and three were renamed. At the species rank, 160 species were added, four were deleted, ten were moved and renamed, and 30 species were renamed. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.

DOI： 10.1007/s00705-020-04731-2

Establishment of Neurospora crassa as a model organism for fungal virology. 国際誌

Shinji Honda, Ana Eusebio-Cope, Shuhei Miyashita, Ayumi Yokoyama, Annisa Aulia, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

Nature communications 11 ( 1 ) 5627 - 5627 2020年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

The filamentous fungus Neurospora crassa is used as a model organism for genetics, developmental biology and molecular biology. Remarkably, it is not known to host or to be susceptible to infection with any viruses. Here, we identify diverse RNA viruses in N. crassa and other Neurospora species, and show that N. crassa supports the replication of these viruses as well as some viruses from other fungi. Several encapsidated double-stranded RNA viruses and capsid-less positive-sense single-stranded RNA viruses can be experimentally introduced into N. crassa protoplasts or spheroplasts. This allowed us to examine viral replication and RNAi-mediated antiviral responses in this organism. We show that viral infection upregulates the transcription of RNAi components, and that Dicer proteins (DCL-1, DCL-2) and an Argonaute (QDE-2) participate in suppression of viral replication. Our study thus establishes N. crassa as a model system for the study of host-virus interactions.

DOI： 10.1038/s41467-020-19355-y

Pathogenetic roles of beet necrotic yellow vein virus RNA5 in the exacerbation of symptoms and yield reduction, development of scab-like symptoms, andRz1-resistance breaking in sugar beet

Tetsuo Tamada, Hirokatsu Uchino, Toshimi Kusume, Minako Iketani-Saito, Sotaro Chiba, Ida Bagus Andika, Hideki Kondo

PLANT PATHOLOGY 70 ( 1 ) 219 - 232 2020年9月

担当区分：最終著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY

Beet necrotic yellow vein virus (BNYVV) generally has a four-segmented positive-sense RNA genome (RNAs 1-4), but some European and most Asian strains have an additional segment, RNA5. This study examined the effect of RNA5 and RNA3 on different sugar beet cultivars using aPolymyxa-mediated inoculation system under field and laboratory conditions. In field tests, the degree of sugar yield served as an index for assessing the virulence of BNYVV strains. Japanese A-II type isolates without RNA5 caused mostly 15%-90% sugar yield reductions, depending on the susceptibility of sugar beet cultivars, whereas the isolates with RNA5 induced more than 90% yield losses in the seven susceptible cultivars, but small yield losses in oneRz1-resistant and Rizor cultivars. However, a laboratory-produced isolate containing RNA5 but lacking RNA3 caused higher yield losses in Rizor than in susceptible plants, and induced scab-like symptoms on the root surface of both susceptible and resistant plants. In laboratory tests, A-II type isolates without RNA5 had low viral RNA accumulation levels in roots of Rizor andRz1-resistant plants at early stages of infection, but in the presence of RNA5, viral RNA3 accumulation levels increased remarkably. This increased RNA3 accumulation was not observed in roots of the WB42 accession with theRz2gene. In contrast, the presence of RNA3 did not affect RNA5 accumulation levels. Collectively, this study demonstrated that RNA5 is involved in the development of scab-like symptoms and the enhancement of RNA3 accumulation, and suggests these characteristics of RNA5 are associated withRz1-resistance breaking.

DOI： 10.1111/ppa.13266

　前のページ - 次のページ　

1
2
3
4
5
6
7
8