Faculty Profiles - KUBOKI Takuo

地域包括ケアの多職種協働の中で, 今まさに『歯科医師』が果たすべき役割は？

Oral Rehabilitation utilizing Several Types of Overdentures

Masumi Shin-ichi, Kuboki Takuo

Annals of Japan Prosthodontic Society 9 ( 4 ) 303 - 303 2017

片岡竜太, 飯島勝矢, 早坂由美子, 松井由美子, 杉山みち子, 窪木拓男

日本歯科医学教育学会雑誌 33 ( 3 ) 149 - 151 2017

矯正歯科治療前および治療済の患者における口腔関連Quality of Lifeと各種歯科矯正学的指標の関連性

加藤ゆい, 川邉紀章, 古森紘基, 片岡伴記, 藤澤厚郎, 大野彩, 窪木拓男, 山城隆, 上岡寛

日本矯正歯科学会大会プログラム・抄録集 75回 226 - 226 2016.11

Assessment of CCN2 Independent Modules Regenerative Capacity on Osteoarthritis and Further Selecting the Most Suitable Among them as a Potential Therapeutic Drug Reviewed

Abdelkader Tarek, Aoyama Eriko, Nishida Takashi, Hattori Takako, Janune Danilo, Hara Emilio S, Ono Mitsuaki, Tabata Yasuhiko, Kuboki Takuo, Kubota Satoshi, Takigawa Masaharu

FASEB JOURNAL 30 2016.4

Assessment of CCN2 Independent Modules Regenerative Capacity on Osteoarthritis and Further Selecting the Most Suitable Among them as a Potential Therapeutic Drug Reviewed

Abdelkader Tarek, Aoyama Eriko, Nishida Takashi, Hattori Takako, Janune Danilo, Hara Emilio S, Ono Mitsuaki, Tabata Yasuhiko, Kuboki Takuo, Kubota Satoshi, Takigawa Masaharu

FASEB JOURNAL 30 2016.4

Highly dynamic self-organizing SMG morphogenesis in 3D culture Reviewed

Mahmoud Farahat, Gulsan Ara Sathi, Hiroaki Taketa, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 2016.3

Language：English Publishing type：Research paper (international conference proceedings) Publisher：Institute of Electrical and Electronics Engineers Inc.

Biological tissues have specific cell patterns and well-organized structures which result from various organogenesis processes. Among which is branching morphogenesis which is the key developmental processes for many glandular tissues including lungs, kidney, salivary gland and liver. Many approaches were suggested to regenerate salivary gland tissue in vitro. Here, we developed a 3D culture technique to study and recapitulate the dynamic forces generated during SMG organogenesis by utilizing time lapse live imaging to monitor the cellular migration and reorganization process of both epithelium and mesenchyme cells.

DOI： 10.1109/MHS.2015.7438259

Scopus

Cellular Reprogramming Using Defined Factors and MicroRNAs International journal

Takanori Eguchi, Takuo Kuboki

STEM CELLS INTERNATIONAL 2016 ( 2016 ) 1 - 12 2016

Language：English Publishing type：Research paper (scientific journal) Publisher：HINDAWI PUBLISHING CORP

Development of human bodies, organs, and tissues contains numerous steps of cellular differentiation including an initial zygote, embryonic stem (ES) cells, three germ layers, and multiple expertized lineages of cells. Induced pluripotent stem (iPS) cells have been recently developed using defined reprogramming factors such as Nanog, Klf5, Oct3/4 (Pou5f1), Sox2, and Myc. This outstanding innovation is largely changing life science and medicine. Methods of direct reprogramming of cells into myocytes, neurons, chondrocytes, and osteoblasts have been further developed using modified combination of factors such as N-myc, L-myc, Sox9, and microRNAs in defined cell/tissue culture conditions. Mesenchymal stem cells (MSCs) and dental pulp stem cells (DPSCs) are also emerging multipotent stem cells with particular microRNA expression signatures. It was shown that miRNA-720 had a role in cellular reprogramming through targeting the pluripotency factor Nanog and induction of DNA methyltransferases (DNMTs). This review reports histories, topics, and idea of cellular reprogramming.

DOI： 10.1155/2016/7530942

Changes in joint space dimension after the correction of Class II division 1 malocclusion. International journal

Alberto Cacho, Tsuyoshi Ono, Takuo Kuboki, Conchita Martin

European journal of orthodontics 37 ( 5 ) 467 - 73 2015.10

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

OBJECTIVE: The aim of this prospective longitudinal investigation was to compare the relationship between the temporomandibular joint (TMJ) condyles and the temporal fossae by means of tomography before and after the orthodontic correction of Class II, division 1 malocclusion using the activator appliance. MATERIALS AND METHODS: The final sample consisted of 26 consecutively treated Class II, division 1 patients (19 boys and 7 girls with an average pre-treatment age of 11 years) who underwent orthodontic treatment by means of an activator appliance. Before treatment all patients were free of signs and symptoms associated to TMJ disorders. Bilateral tomographic records before and after treatment were taken and analyzed. Outlines of the condyle and temporal fossa were automatically determined by an edge-detection protocol, and the minimum joint space dimension was automatically measured every 2 degrees. For further analysis, the joint space was divided into anterior, superior, and posterior joint spaces. RESULTS: The average treatment time with the activator appliance was 366 days. In all subjects, activator treatment resulted in a Class I dental arch relationship. After activator treatment, no differences were found in the joint space measurements in any direction. Comparisons between the right and left condyles were not significantly different. CONCLUSIONS: Joint space dimension in Class II division I children was similar before and after treatment in both TMJs.

DOI： 10.1093/ejo/cju091

Concealed Jiggling Effect: A Novel Risk Factor for Complications of Implant-Supported Fixed Partial Denture. International journal

Ryu Nakajima, Kenji Maekawa, Wataru Sonoyama, Akihiro Ueda, Takuo Kuboki

The Journal of oral implantology 41 ( 4 ) 491 - 4 2015.8

A risk factor analysis of accumulated postoperative pain and swelling sensation after dental implant surgery using a cellular phone-based real-time assessment.

Ryuta Kuroi, Hajime Minakuchi, Emilio Satoshi Hara, Aya Kawakami, Kenji Maekawa, Hiroki Okada, Takuo Kuboki

Journal of prosthodontic research 59 ( 3 ) 194 - 8 2015.7

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER IRELAND LTD

PURPOSE: The purpose of this study was to identify the related risk factors of dental implant accumulated postoperative pain and swelling by cellular phone-based assessment. METHODS: Subjects were a consecutive series of patients who received oral implant surgery at Okayama University Hospital. Cellular phone-based questionnaire was sent at pre-set schedule to each subject every 2h on the day of surgery, and every 24h from the 2nd to 7th day post-surgery. Subjects replied in real-time the pain and swelling levels at the operated sites by an 11- and 4-grade rating-scale questionnaire. Overall intensity of individual pain and swelling was calculated by means of area under curve that drew by their time-dependent changes. Predictor variables were age, gender, presence of diabetes mellitus and/or hypertension (DM/HT), history of implant surgery, number of inserted implants, flap operation, surgical duration, pre-surgery anxiety, osteoplasty, bone quality, premedication, dosage of prescribed analgesics and local anesthesia and accumulated postoperative pain/swelling. Compliance rate and risk factors correlated with accumulated postoperative pain and swelling were calculated by multiple regression analysis. RESULTS: Final subjects were 18 females and 7 male (mean age: 59.3±7.32 yrs). Significant factors correlated with accumulated postoperative pain were DM/HT, surgical duration, premedication, bone quality, pre-surgery anxiety and postoperative swelling (R(2)=0.769, p=0.001, 0.013, 0.032, 0.007, 0.035 and 0.007, respectively). Meanwhile, significant factors associated with postoperative swelling were postoperative pain, DM/HT and bone quality (R(2)=0.365, p=0.002, 0.004, 0.008, respectively). CONCLUSION: These results suggested DM/HT and bone quality are correlated to overall intensity of postoperative pain and swelling.

DOI： 10.1016/j.jpor.2015.05.003

平成26年度にODAPUSプログラムで岡山大学に留学をした3ヶ国4校の学生の意識調査

藤田麻里子, 此内浩信, 柳文修, 久富美紀, 村上純, 窪木拓男, 宮脇卓也, 仲野道代, 浅海淳一

日本歯科医学教育学会総会・学術大会プログラム・抄録集 34回 143 - 143 2015.7

Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro. International journal

Hiroaki Taketa, Gulsan Ara Sathi, Mahmoud Farahat, Kazi Anisur Rahman, Takayoshi Sakai, Yoshiaki Hirano, Takuo Kuboki, Yasuhiro Torii, Takuya Matsumoto

Scientific reports 5 11468 - 11468 2015.6

Language：English Publishing type：Research paper (scientific journal) Publisher：NATURE PUBLISHING GROUP

In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication.

DOI： 10.1038/srep11468

Interdisciplinary orthodontic treatment for a patient with generalized aggressive periodontitis: Assessment of IgG antibodies to identify type of periodontitis and correct timing of treatment. International journal

Yoshihito Ishihara, Kazuya Tomikawa, Toru Deguchi, Tadashi Honjo, Koji Suzuki, Takayuki Kono, Takuo Kuboki, Hiroshi Kamioka, Shogo Takashiba, Takashi Yamashiro

American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 147 ( 6 ) 766 - 80 2015.6

Language：English Publishing type：Research paper (scientific journal) Publisher：MOSBY-ELSEVIER

Aggressive periodontitis is a great challenge to clinicians when providing orthodontic treatment because of the potential for progression of periodontal disease. In this article, we report the successful comprehensive orthodontic treatment of bimaxillary protrusion and severe crowding in an adult with generalized aggressive periodontitis. A woman, aged 22 years 7 months, with a chief complaint of incisal crowding was diagnosed with a skeletal Class I malocclusion associated with severe anterior crowding, possibly worsened by generalized aggressive periodontitis. In addition to a periodontal examination, a blood IgG antibody titer analysis and microbiologic examination for periodontal pathogens were used to diagnose the type of periodontal disease and determine the proper timing to initiate orthodontic treatment. The total active treatment period was 28 months, followed by periodontal prostheses and regeneration therapy. Consequently, satisfactory facial profile, occlusion, and periodontal health were maintained for at least 36 months. These results indicate that efficient screening is important for providing successful orthodontic treatment in patients with advanced periodontal disease. This report also demonstrates the diagnostic importance of blood IgG antibody titer assays and microbiologic examinations to detect periodontal pathogens.

DOI： 10.1016/j.ajodo.2014.09.022

健常者における睡眠時の覚醒反応と随伴運動との関連

三木春奈, 水口一, 上枝麻友, 重本修伺, 鈴木善貴, 前川賢治, 松香芳三, 窪木拓男

日本補綴歯科学会誌 7 ( 特別号 ) 248 - 248 2015.5

In silico comparison of the reproducibility of full-arch implant provisional restorations to final restoration between a 3D Scan/CAD/CAM technique and the conventional method.

Takuya Mino, Kenji Maekawa, Akihiro Ueda, Shizuo Higuchi, Junichi Sejima, Tetsuo Takeuchi, Emilio Satoshi Hara, Aya Kimura-Ono, Wataru Sonoyama, Takuo Kuboki

Journal of prosthodontic research 59 ( 2 ) 152 - 8 2015.4

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER IRELAND LTD

PURPOSE: The aim of this article was to investigate the accuracy in the reproducibility of full-arch implant provisional restorations to final restorations between a 3D Scan/CAD/CAM technique and the conventional method. METHODS: We fabricated two final restorations for rehabilitation of maxillary and mandibular complete edentulous area and performed a computer-based comparative analysis of the accuracy in the reproducibility of the provisional restoration to final restoration between a 3D scanning and CAD/CAM (Scan/CAD/CAM) technique and the conventional silicone-mold transfer technique. Final restorations fabricated either by the conventional or Scan/CAD/CAM method were successfully installed in the patient. The total concave/convex volume discrepancy observed with the Scan/CAD/CAM technique was 503.50mm(3) and 338.15 mm(3) for maxillary and mandibular implant-supported prostheses (ISPs), respectively. On the other hand, total concave/convex volume discrepancy observed with the conventional method was markedly high (1106.84 mm(3) and 771.23 mm(3) for maxillary and mandibular ISPs, respectively). CONCLUSIONS: The results of the present report suggest that Scan/CAD/CAM method enables a more precise and accurate transfer of provisional restorations to final restorations compared to the conventional method.

DOI： 10.1016/j.jpor.2014.12.001

咬合違和感を訴える患者の実態に関する多施設実態調査 Reviewed

澁谷智明, 和気裕之, 玉置勝司, 島田淳, 古谷野潔, 鱒見進一, 窪木拓男, 皆木省吾, 貞森紳丞, 矢谷博文, 藤澤政紀, 林勝彦, 玉井和樹, 成田紀之, 原節宏, 馬場一美, 尾口仁志, 金村清孝, 山口泰彦, 西川洋二, 塚原宏泰, 松香芳三, 葉山莉香

日顎誌 26 ( 3 ) 196 - 203 2015.2

Language：Japanese Publisher：(一社)日本顎関節学会

目的:多施設にて咬合違和感を訴える患者に関するデータ収集を行い、その実態を調査することを目的とした。対象および方法:各施設を受診した咬合違和感を訴える患者202症例中、調査票で転帰が明らかな180症例を対象とした。患者調査票を作成し、受診までの項目(性別、年齢、病悩期間、主訴の分類、咬合違和感を感じる歯、咬合違和感を感じる部位、咬合違和感を感じる歯の状態、前医でこれまでに受けた治療法)について分析した。結果:性別では男性37名、女性143名で女性に多かった。年齢の中央値(25パーセンタイル、75パーセンタイル)は55.0(42.0、65.0)歳であった(18〜86歳)。また病悩期間の中央値は5(1、13)ヵ月で発症後6ヵ月未満が著明に多かった(発症直後〜360ヵ月)。主訴は「咬頭嵌合位の歯の接触状態に関する訴え」が最も多かった。咬合違和感を感じる歯は大臼歯が多かったが前歯にも認められた。咬合違和感を感じる部位では片側だけでなく、両側の場合などさまざまであったが、全体的に感じる患者も多かった。咬合違和感を感じる歯の状態は金属の補綴装置を装着されている場合が多かったが、天然歯の場合も多かった。前医で行われた治療は補綴歯科治療が多かった。結語:性別、年齢などはいままでの報告と同様であったが、病悩期間はやや異なっていた。また患者は、さまざまな部位で治療後の歯だけでなく天然歯にも違和感を感じていた。(著者抄録)

DOI： 10.11246/gakukansetsu.26.196

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2014&ichushi_jid=J02499&link_issn=&doc_id=20150205190003&doc_link_id=10.11246%2Fgakukansetsu.26.196&url=https%3A%2F%2Fdoi.org%2F10.11246%2Fgakukansetsu.26.196&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

The Effect of Tooth Loss and/or Oral Function to Nutritional Status -Assessment of These Relationship

Takeshi KIKUTANI, Mitsuyoshi YOSHIDA, Takeo SUGA, Toshihide KIMURA, Fumiyo TAMURA, Takuo KUBOKI

日本歯科医学会誌 34 59 - 63 2015

Food Ingestion/Mastication/Swallowing Evaluation Method Corre- sponding to the Stage of Patients and Its Application: Feasibility of an Oral Function Test

Mami INOUE-MINAKUCHI, Kenji MAEKAWA, Takeshi KIKUTANI, Fumiyo TAMURA, Mitsuyoshi YOSHIDA, Tomohisa ONO, Yasushi TAMADA, Hisatomo KONDO, Katsuhiko KIMOTO, Shuji OHKAWA, Yuji SATO, Hiroshi SUZUKI, Misao KAWARA, Takuo KUBOKI

34 94 - 98 2015

Topical application of lithium chloride on the pulp induces dentin regeneration. International journal

Kazuya Ishimoto, Satoru Hayano, Takeshi Yanagita, Hiroshi Kurosaka, Noriaki Kawanabe, Shinsuke Itoh, Mitsuaki Ono, Takuo Kuboki, Hiroshi Kamioka, Takashi Yamashiro

PloS one 10 ( 3 ) e0121938 2015

Language：English Publishing type：Research paper (scientific journal) Publisher：PUBLIC LIBRARY SCIENCE

We herein describe a novel procedure for dentin regeneration that mimics the biological processes of tooth development in nature. The canonical Wnt signaling pathway is an important regulator of the Dentin sialophosphoprotein (Dspp) expression. Our approach mimics the biological processes underlying tooth development in nature and focuses on the activation of canonical Wnt signaling to trigger the natural process of dentinogenesis. The coronal portion of the dentin and the underlying pulp was removed from the first molars. We applied lithium chloride (LiCl), an activator of canonical Wnt signaling, on the amputated pulp surface to achieve transdifferentiation toward odontoblasts from the surrounding pulpal cells. MicroCT and microscopic analyses demonstrated that the topical application of LiCl induced dentin repair, including the formation of a complete dentin bridge. LiCl-induced dentin is a tubular dentin in which the pulp cells are not embedded within the matrix, as in primary dentin. In contrast, a dentin bridge was not induced in the control group treated with pulp capping with material carriers alone, although osteodentin without tubular formation was induced at a comparatively deeper position from the pulp exposure site. We also evaluated the influence of LiCl on differentiation toward odontoblasts in vitro. In the mDP odontoblast cell line, LiCl activated the mRNA expression of Dspp, Axin2 and Kallikrein 4 (Klk4) and downregulated the Osteopontin (Osp) expression. These results provide a scientific basis for the biomimetic regeneration of dentin using LiCl as a new capping material to activate dentine regeneration.

DOI： 10.1371/journal.pone.0121938