Faculty Profiles - Uchiyama Jumpei

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Evaluation of risk and benefit in thermal effusivity sensor for monitoring lubrication process in pharmaceutical product manufacturing Reviewed

Jumpei Uchiyama, Yoshiteru Kato, Yoshifumi Uemoto

DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY 40 ( 8 ) 999 - 1004 2014.8

Language：English Publisher：TAYLOR & FRANCIS LTD

In the process design of tablet manufacturing, understanding and control of the lubrication process is important from various viewpoints. A detailed analysis of thermal effusivity data in the lubrication process was conducted in this study. In addition, we evaluated the risk and benefit in the lubrication process by a detailed investigation. It was found that monitoring of thermal effusivity detected mainly the physical change of bulk density, which was changed by dispersal of the lubricant and the coating powder particle by the lubricant. The monitoring of thermal effusivity was almost the monitoring of bulk density, thermal effusivity could have a high correlation with tablet hardness. Moreover, as thermal effusivity sensor could detect not only the change of the conventional bulk density but also the fractional change of thermal conductivity and thermal capacity, two-phase progress of lubrication process could be revealed. However, each contribution of density, thermal conductivity, or heat capacity to thermal effusivity has the risk of fluctuation by formulation. After carefully considering the change factor with the risk to be changed by formulation, thermal effusivity sensor can be a useful tool for monitoring as process analytical technology, estimating tablet hardness and investigating the detailed mechanism of the lubrication process.

DOI： 10.3109/03639045.2013.795581

ヒト正常肝および脂肪肝におけるオートファジーマーカータンパク質であるmicrotubule-associated protein 1 light chain 3の免疫組織化学的研究(Immunohistochemical study of the autophagy marker microtubule-associated protein 1 light chain 3 in normal and steatotic human livers)

Kashima Jumpei, Shintani-Ishida Kaori, Nakajima Makoto, Maeda Hideyuki, Unuma Kana, Uchiyama Yasuo, Yoshida Ken-ichi

Hepatology Research 44 ( 7 ) 779 - 787 2014.7

Language：English Publisher：John Wiley & Sons Australia, Ltd

オートファジーは脂肪滴(LD)の代謝回転に関連している。Adipose differentiation-related protein(ADRP)およびmicrotubule-associated protein 1 light chain 3(LC3)は、それぞれLDおよびオートファゴソームをモニターしている。ADRPおよびLC3の免疫組織化学的染色法によりLDとオートファジーをモニターできるか検討し、もしできる場合には、オートファジーがLDの代謝回転に関係しているかについて検討した。検死で得たパラフィン包埋された様々な重症度のヒト脂肪肝のLC3を、免疫組織化学法で検査した。オートファジーとLD代謝回転の関係を解明するため、抗LC3抗体および抗ADRP抗体を用いて二重染色免疫蛍光顕微鏡による検査を実施した。その結果、LC3免疫組織化学検査ではヒト正常肝において、中心静脈の周囲に再現性のある多くの斑点が描出された。脂肪肝の進行に伴いLC3免疫染色の広がりは減少した。ADRPおよびLC3の免疫蛍光二重染色によりADRP陽性領域とLC3陽性領域は逆相関を示し、小型LDにおいてADRPとLC3は共に局在化していたが、大型LDでは認められなかった。結論として、オートファジーの障害が脂肪症を促進すること、そしてオートファジーがLDの代謝回転に関与している可能性が示唆された。

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Other Link： https://search.jamas.or.jp/default/link?pub_year=2014&ichushi_jid=J04726&link_issn=&doc_id=20140718260009&doc_link_id=10.1111%2Fhepr.12183&url=https%3A%2F%2Fdoi.org%2F10.1111%2Fhepr.12183&type=Crossref&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00002_2.gif

Development of new punch shape to replicate scale-up issues in laboratory tablet press II: a new design of punch head to emulate consolidation and dwell times in commercial tablet press. Reviewed International journal

Shigeru Aoki, Jumpei Uchiyama, Manabu Ito

Journal of pharmaceutical sciences 103 ( 6 ) 1921 - 7 2014.6

Language：English Publisher：WILEY-BLACKWELL

Differences between laboratory and commercial tablet presses are frequently observed during scale-up of tableting process. These scale-up issues result from the differences in total compression time that is the sum of consolidation and dwell times. When a lubricated blend is compressed into tablets, the tablet thickness produced by the commercial tablet press is often thicker than that by a laboratory tablet press. A new punch shape design, designated as shape adjusted for scale-up (SAS), was developed and used to demonstrate the ability to replicate scale-up issues in commercial-scale tableting processes. It was found that the consolidation time can be slightly shortened by changing the vertical curvature of the conventional punch head rim. However, this approach is not enough to replicate the consolidation time. A secondary two-stage SAS punch design and an embossed punch head was designed to replicate the consolidation and dwell times on a laboratory tablet press to match those of a commercial tablet press. The resulting tablet thickness using this second SAS punch on a laboratory tablet press was thicker than when using a conventional punch in the same laboratory tablet press. The secondary SAS punches are more useful tools for replicating and understanding potential scale-up issues. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci.

DOI： 10.1002/jps.23989

Examination of generalized transduction among Staphylococcus spp., using a novel phage S6

UCHIYAMA JUMPEI, TAKEMURA-UCHIYAMA IYO, SAKAGUCHI YOSHIHIKO, MISAWA NAOAKI, DAIBATA MASANORI, MATSUZAKI SHIGENOBU

日本細菌学雑誌 69 ( 1 ) 236 - 236 2014.2

緑膿菌ファージKPP21,KPP22,KPP23,KPP25の網羅的構造タンパク質解析

重久立, 内山淳平, 宮田怜奈, 山口琴絵, 内山伊代, 氏原隆子, 大畑雅典, 松崎茂展

日本細菌学雑誌 69 ( 1 ) 139 - 139 2014.2

緑膿菌ファージKPP22吸着能変異ファージの解析

宮田怜奈, 内山淳平, 山口琴絵, 重久立, 内山伊代, 氏原隆子, 大畑雅典, 松崎茂展

日本細菌学雑誌 69 ( 1 ) 138 - 138 2014.2

Phage therapy experiment against staphylococcal lung-derived septic mouse model

UCHIYAMA IYO, UCHIYAMA JUMPEI, SAKAGUCHI YOSHIHIKO, UJIHARA TAKAKO, DAIBATA MASANORI, MATSUZAKI SHIGENOBU

日本細菌学雑誌 69 ( 1 ) 139 - 139 2014.2

緑膿菌ファージの分離とそれらのゲノム塩基配列

山口琴絵, 内山淳平, 宮田怜奈, 重久立, 内山伊代, 氏原隆子, 大畑雅典, 松崎茂展

日本細菌学雑誌 69 ( 1 ) 138 - 138 2014.2

Estimation of Helicobacter pylori KHP30-like phage ecology

MATSUZAKI SHIGENOBU, UCHIYAMA JUMPEI, TAKEUCHI HIROAKI, SAKAGUCHI YOSHIHIKO, UCHIYAMA-TAKEMURA IYO, UJIHARA TAKAKO, DAIBATA MASANORI

日本細菌学雑誌 69 ( 1 ) 237 2014.2

ピロリ菌におけるKHP30様ファージの存在様式(Estimation of Helicobacter pylori KHP30-like phage ecology)

松崎茂展, 内山淳平, 竹内啓晃, 阪口義彦, 内山伊代[竹村], 氏原隆子, 大畑雅典

日本細菌学雑誌 69 ( 1 ) 237 - 237 2014.2

Clostridium argentinenseにおけるG型ボツリヌス神経毒素遺伝子をコードするプラスミドの分析(Analysis of botulinum neurotoxin type G gene-encoding plasmid in Clostridium argentinense)

阪口義彦, 内山淳平, 鈴木智典, 山本由弥子, 小椋義俊, 細見晃司, 松崎茂展, 林哲也, 小崎俊司, 小熊惠二

日本細菌学雑誌 69 ( 1 ) 236 - 236 2014.2

阪口義彦, 内山淳平, 鈴木智典, 山本由弥子, 小椋義俊, 細見晃司, 松崎茂展, 林哲也, 小崎俊司, 小熊惠二

日本細菌学雑誌 69 ( 1 ) 236 - 236 2014.2

黄色ブドウ球菌性肺由来敗血症マウスモデルにおけるファージ療法の検討(Phage therapy experiment against staphylococcal lungderived septic mouse model)

内山伊代, 内山淳平, 阪口義彦, 氏原隆子, 大畑雅典, 松崎茂展

日本細菌学雑誌 69 ( 1 ) 139 - 139 2014.2

新規ファージS6の分離とブドウ球菌における普遍形質導入の検討(Examination of generalized transduction among Staphylococcus spp., using a novel phage S6)

内山淳平, 内山伊代, 阪口義彦, 三澤尚明, 大畑雅典, 松崎茂展

日本細菌学雑誌 69 ( 1 ) 236 - 236 2014.2

A novel approach to a fine particle coating using porous spherical silica as core particles

Ishida, M., Uchiyama, J., Isaji, K., Suzuki, Y., Ikematsu, Y., Aoki, S.

Drug Development and Industrial Pharmacy 40 ( 8 ) 1054 - 1064 2014.1

The applicability of porous spherical silica (PSS) was evaluated as core particles for pharmaceutical products by comparing it with commercial core particles such as mannitol (NP-108), sucrose and microcrystalline cellulose spheres. We investigated the physical properties of core particles, such as particle size distribution, flow properties, crushing strength, plastic limit, drying rate, hygroscopic property and aggregation degree. It was found that PSS was a core particle of small particle size, low friability, high water adsorption capacity, rapid drying rate and lower occurrence of particle aggregation, although wettability is a factor to be carefully considered. The aggregation and taste-masking ability using PSS and NP-108 as core particles were evaluated at a fluidized-bed coating process. The functional coating under the excess spray rate shows different aggregation trends and dissolution profiles between PSS and NP-108; thereby, exhibiting the formation of uniform coating under the excess spray rate in the case of PSS. This expands the range of the acceptable spray feed rates to coat fine particles, and indicates the possibility of decreasing the coating time. The results obtained in this study suggested that the core particle, which has a property like that of PSS, was useful in overcoming such disadvantages as large particle size, which feels gritty in oral cavity; particle aggregation; and the long coating time of the particle coating process. These results will enable the practical fine particle coating method by increasing the range of optimum coating conditions and decreasing the coating time in fluidized bed technology. © 2014 Informa Healthcare USA, Inc.

DOI： 10.3109/03639045.2013.807277

Scopus

PubMed

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Development of new shaped punch to predict scale-up issue in tableting process. Reviewed International journal

Shigeru Aoki, Jumpei Uchiyama, Manabu Ito

Journal of pharmaceutical sciences 103 ( 1 ) 235 - 40 2014.1

Language：English

Scale-up issues in the tableting process, such as capping, sticking, or differences in tablet thickness, are often observed at the commercial production scale. A new shaped punch, named the size adjusted for scale-up (SAS) punch, was created to estimate scale-up issues seen between laboratory scale and commercial scale tableting processes. The SAS punch's head shape was designed to replicate the total compression time of a laboratory tableting machine to that of a commercial tableting machine. Three different lubricated blends were compressed into tablets using a laboratory tableting machine equipped with SAS punches, and any differences in tablet thickness or capping phenomenon were observed. It was found that the new shaped punch could be used to replicate scale-up issues observed in the commercial tableting machine. The SAS punch was shown to be a useful tool to estimate scale-up issues in the tableting process.

DOI： 10.1002/jps.23781

Scopus

PubMed

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Draft genome sequence of Clostridium botulinum type B strain Osaka05, isolated from an infant patient with botulism in Japan Reviewed

Yoshihiko Sakaguchi, Koji Hosomi, Jumpei Uchiyama, Yoshitoshi Ogura, Kaoru Umeda, Masakiyo Sakaguchi, Tomoko Kohda, Masafumi Mukamoto, Naoaki Misawa, Shigenobu Matsuzaki, Tetsuya Hayashi, Shunji Kozaki

Genome Announcements 2 ( 1 ) 2014

Helicobacter pylori基礎研究の最前線 2.病原因子 Helicobacter pyloriのファージに関する基礎摘研究

竹内啓晃, 内山淳平, 松崎茂展, 森本徳仁, 杉浦哲朗

Helicobacter Res 17 ( 5 ) 400 - 405 2013.10

【Helicobacter pylori基礎研究の最前線】病原因子 Helicobacter pyloriのファージに関する基礎的研究

竹内啓晃, 内山淳平, 松崎茂展, 森本徳仁, 杉浦哲朗

Helicobacter Research 17 ( 5 ) 400 - 405 2013.10

Language：Japanese Publisher：(株)先端医学社

尾部のないファージの分離率はわずか約4%とまれである。そのなかに球形ファージを含むさまざまな分類がある。われわれはHelicobacter pylori(H.pylori)が自然放出している二つの球形ファージ(KHP30とKHP40)を発見した。脂質を含むparticleは線状の約26kbp dsDNAで頭部直径約70nmであった。菌体内ではゲノムにintegrateされておらず、おそらくepisomal formで存在するpseudolysogenyで、burst sizeは約13、宿主域は約50%であった。ゲノムおよび性状解析の結果、従来の球形ファージfamily(CorticoviridaeやTectiviridae)には分類できない新規な球形ファージと考えた。(著者抄録)

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Other Link： http://search.jamas.or.jp/link/ui/2014038434

Helicobacter pylori ファージに関する基礎的研究 Invited

竹内啓晃, 内山淳平, 松﨑茂展, 森本徳仁, 杉浦哲朗

Helicobacter Research 17 ( 5 ) 28 - 33 2013.10

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