研究者詳細 - 山地　直樹

論文 - 山地　直樹

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Effects of polygalacturonase overexpression on pectin distribution in the elongation zones of roots under aluminium stress. 国際誌

Teruki Nagayama, Akane Tatsumi, Atsuko Nakamura, Naoki Yamaji, Shinobu Satoh, Jun Furukawa, Hiroaki Iwai

AoB PLANTS 14 ( 2 ) plac003 2022年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

The roots of many plant species contain large amounts of pectin and it contributes to the formation of the rhizosphere. In the present study, the relationship between the root-tip pectin content and aluminium (Al) tolerance in wild-type (WT) and demethylesterified pectin degradation enzyme gene overexpressor (OsPG2-FOX) rice lines was compared. OsPG2-FOX rice showed reduced pectin content in roots, even under control conditions; Al treatment reduced root elongation and the pectin content in the root elongation zone. Wild-type rice showed more pectin accumulation in the root elongation zone after Al treatment. Relative to WT rice, OsPG2-FOX rice showed more Al accumulation in the root elongation zone. These results indicate that the amount of pectin influences Al tolerance and that the distribution of pectin in the root elongation zone inhibits Al accumulation in rice roots. Pectin accumulation in cell walls in the root elongation zone may play a role in protecting rice plants from the Al-induced inhibition of root elongation by regulating pectin distribution.

DOI： 10.1093/aobpla/plac003

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A crucial role for a node-localized transporter, HvSPDT, in loading phosphorus into barley grains. 国際誌

Mian Gu, Hengliang Huang, Hiroshi Hisano, Guangda Ding, Sheng Huang, Namiki Mitani-Ueno, Kengo Yokosho, Kazuhiro Sato, Naoki Yamaji, Jian Feng Ma

The New phytologist 2022年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Grains are the major sink of phosphorus (P) in cereal crops, accounting for 60-85% of total plant P, but the mechanisms underlying P loading into the grains are poorly understood. We functionally characterized a transporter gene required for the distribution of P to the grains in barley (Hordeum vulgare), HvSPDT (SULTR-like phosphorus distribution transporter). HvSPDT encoded a plasma membrane-localized Pi/H+ cotransporter. It was mainly expressed in the nodes at both the vegetative and reproductive stages. Furthermore, its expression was induced by inorganic phosphate (Pi) deficiency. In the nodes, HvSPDT was expressed in both the xylem and phloem region of enlarged and diffuse vascular bundles. Knockout of HvSPDT decreased the distribution of P to new leaves, but increased the distribution to old leaves at the vegetative growth stage under low P supply. However, knockout of HvSPDT did not alter the redistribution of P from old to young organs. At the reproductive stage, knockout of HvSPDT significantly decreased P allocation to the grains, resulting in a considerable reduction in grain yield, especially under P-limited conditions. Our results indicate that node-based HvSPDT plays a crucial role in loading P into barley grains through preferentially distributing P from the xylem and further to the phloem.

DOI： 10.1111/nph.18057

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Boron uptake in rice is regulated post-translationally via a clathrin-independent pathway

Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Ji Feng Shao, Namiki Mitani-Ueno, Jian Feng Ma

Plant Physiology 188 ( 3 ) 1649 - 1664 2021年12月

掲載種別：研究論文（学術雑誌）出版者・発行元：Oxford University Press (OUP)

<title>Abstract</title>
Uptake of boron (B) in rice (Oryza sativa) is mediated by the Low silicon rice 1 (OsLsi1) channel, belonging to the NOD26-like intrinsic protein III subgroup, and the efflux transporter B transporter 1 (OsBOR1). However, it is unknown how these transporters cooperate for B uptake and how they are regulated in response to B fluctuations. Here, we examined the response of these two transporters to environmental B changes at the transcriptional and posttranslational level. OsBOR1 showed polar localization at the proximal side of both the exodermis and endodermis of mature root region, forming an efficient uptake system with OsLsi1 polarly localized at the distal side of the same cell layers. Expression of OsBOR1 and OsLsi1 was unaffected by B deficiency and excess. However, although OsLsi1 protein did not respond to high B at the protein level, OsBOR1 was degraded in response to high B within hours, which was accompanied with a significant decrease of total B uptake. The high B-induced degradation of OsBOR1 was inhibited in the presence of MG-132, a proteasome inhibitor, without disturbance of the polar localization. In contrast, neither the high B-induced degradation of OsBOR1 nor its polarity was affected by induced expression of dominant-negative mutated dynamin-related protein 1A (OsDRP1AK47A) or knockout of the mu subunit (AP2M) of adaptor protein-2 complex, suggesting that clathrin-mediated endocytosis is not involved in OsBOR1 degradation and polar localization. These results indicate that, in contrast to Arabidopsis thaliana, rice has a distinct regulatory mechanism for B uptake through clathrin-independent degradation of OsBOR1 in response to high B.

DOI： 10.1093/plphys/kiab575

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Metalloid transporters and their regulation in plants

Naoki Yamaji, Jian Feng Ma

Plant Physiology 187 ( 4 ) 1929 - 1939 2021年12月

Zinc Transport in Rice: Dilemma between optimal plant requirement and human nutrition. 国際誌

Sheng Huang, Naoki Yamaji, Jian Feng Ma

Journal of experimental botany 2021年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Zinc (Zn) is an essential micronutrient for both plants and animals, while Zn deficiency in crops and humans is a global problem affecting crop productivity and human health. Since plants and humans differ in their Zn requirement, there is a dilemma between plant nutrition and human nutrition. In this review, we focus on the transport system of Zn from soil to grain in rice (Oryza sativa), which is a major dietary source of Zn for people subsiding on rice-based diets. We describe transporters belonging to different family, which are involved in the uptake, vacuolar sequestration, root-to-shoot translocation and distribution of Zn. We also discuss the possible regulation mechanism of these transporters. Several examples are given on enhancing Zn accumulation and bioavailability in rice grains through manipulation of genes highly expressed in the nodes, where Zn is highly deposited. We finally provide our perspectives toward breeding rice cultivars with both increased tolerance to Zn-deficiency stress and high Zn density in rice grains.

DOI： 10.1093/jxb/erab478

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Structural basis for high selectivity of a rice silicon channel Lsi1. 国際誌

Yasunori Saitoh, Namiki Mitani-Ueno, Keisuke Saito, Kengo Matsuki, Sheng Huang, Lingli Yang, Naoki Yamaji, Hiroshi Ishikita, Jian-Ren Shen, Jian Feng Ma, Michihiro Suga

Nature communications 12 ( 1 ) 6236 - 6236 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Silicon (Si), the most abundant mineral element in the earth's crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids.

DOI： 10.1038/s41467-021-26535-x

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Lateral roots but not root hairs contribute to high uptake of manganese and cadmium in rice. 国際誌

En Yu, Naoki Yamaji, Chuanzao Mao, Hua Wang, Jian Feng Ma

Journal of experimental botany 72 ( 20 ) 7219 - 7228 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Rice (Oryza sativa L.) can accumulate high manganese (Mn) in the shoots through uptake by the roots, which consist of crown roots, lateral roots and root hairs. We investigated the role of lateral roots and root hairs in Mn and cadmium (Cd) uptake by using two indica rice mutants defective in formation of lateral roots (osiaa11) and root hairs (osrhl1). The uptake of Mn and Cd in osiaa11 was significantly lower than that in wild type 'Kasalath', but there was no difference between wild type and osrhl1. Furthermore, a kinetic study showed that Mn uptake in osiaa11 was much lower than that in wild type and osrhl1 across a wide range of Mn concentrations. The role of lateral roots in Mn and Cd uptake was further confirmed in a japonica rice mutant defective in lateral root formation. We found that expression of Mn transporter gene Natural Resistance-Associated Macrophage Protein 5 (OsNRAMP5), but not of Metal Tolerance Protein 9 (OsMTP9), was lower in osiaa11 than in wild type; however, there were no differences between osrhl1 and the wild type. Immunostaining showed that OsNRAMP5 and OsMTP9 were localized in the exodermis and endodermis of crown roots and lateral roots, but not in the root hairs. Taken together, our results indicate that lateral roots, but not root hairs, play an important role in high Mn and Cd uptake in rice.

DOI： 10.1093/jxb/erab329

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Effectiveness of Create ML in microscopy image classifications: a simple and inexpensive deep learning pipeline for non-data scientists. 国際誌

Kiyotaka Nagaki, Tomoyuki Furuta, Naoki Yamaji, Daichi Kuniyoshi, Megumi Ishihara, Yuji Kishima, Minoru Murata, Atsushi Hoshino, Hirotomo Takatsuka

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 29 ( 3-4 ) 361 - 371 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Observing chromosomes is a time-consuming and labor-intensive process, and chromosomes have been analyzed manually for many years. In the last decade, automated acquisition systems for microscopic images have advanced dramatically due to advances in their controlling computer systems, and nowadays, it is possible to automatically acquire sets of tiling-images consisting of large number, more than 1000, of images from large areas of specimens. However, there has been no simple and inexpensive system to efficiently select images containing mitotic cells among these images. In this paper, a classification system of chromosomal images by deep learning artificial intelligence (AI) that can be easily handled by non-data scientists was applied. With this system, models suitable for our own samples could be easily built on a Macintosh computer with Create ML. As examples, models constructed by learning using chromosome images derived from various plant species were able to classify images containing mitotic cells among samples from plant species not used for learning in addition to samples from the species used. The system also worked for cells in tissue sections and tetrads. Since this system is inexpensive and can be easily trained via deep learning using scientists' own samples, it can be used not only for chromosomal image analysis but also for analysis of other biology-related images.

DOI： 10.1007/s10577-021-09676-z

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Buckwheat FeNramp5 Mediates High Manganese Uptake in Roots.

Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

Plant & cell physiology 62 ( 4 ) 600 - 609 2021年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

Manganese (Mn) is an essential element for plant growth and development, but transporters required for Mn uptake have only been identified in a few plant species. Here, we functionally characterized a member of the natural resistance-associated macrophage proteins (Nramps) family, FeNramp5 in buckwheat (Fagopyrum esculentum Moench), which is known as a species well adapted to acidic soils. FeNramp5 was mainly expressed in the roots, and its expression was upregulated by the deficiency of Mn and Fe. Furthermore, spatial and tissue-specific expression analysis showed that FeNramp5 was expressed in all tissues of the basal root regions. FeNramp5-GFP protein was localized to the plasma membrane when transiently expressed in buckwheat leaf protoplast. FeNramp5 showed the transport activity for Mn2+ and Cd2+ but not for Fe2+ when expressed in yeast. Furthermore, the transport activity for Mn2+ was higher in yeast expressing FeNramp5 than in yeast expressing AtNramp1. FeNramp5 was also able to complement the phenotype of Arabidopsis atnramp1 mutant in terms of the growth and accumulation of Mn and Cd. The absolute expression level of AtNramp1 was comparable to that of FeNramp5 in the roots, but buckwheat accumulated higher Mn than Arabidopsis when grown under the same condition. Further analysis showed that at least motif B in FeNramp5 seems important for its high transport activity for Mn. These results indicate that FeNramp5 is a transporter for the uptake of Mn and Cd and its higher transport activity for Mn is probably associated with higher Mn accumulation in buckwheat.

DOI： 10.1093/pcp/pcaa153

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Lysine Ketoglutarate Reductase Trans-Splicing Related 1 Is Involved In A Temperature-Dependent Root Growth in Rice. 国際誌

En Yu, Naoki Yamaji, Keiich Mochida, Ivan Galis, Kanatani Asaka, Jian Feng Ma

Journal of experimental botany 72 ( 18 ) 6336 - 6349 2021年5月

Role of a vacuolar iron transporter OsVIT2 in the distribution of iron to rice grains

Jing Che, Naoki Yamaji, Jian Feng Ma

NEW PHYTOLOGIST 230 ( 3 ) 1049 - 1062 2021年5月

Fine regulation system for distribution of boron to different tissues in rice 国際誌

Ji Feng Shao, Naoki Yamaji, Sheng Huang, Jian Feng Ma

NEW PHYTOLOGIST 230 ( 2 ) 656 - 668 2021年4月

A transporter for delivering zinc to the developing tiller bud and panicle in rice

Shuai Mu, Naoki Yamaji, Akimasa Sasaki, Le Luo, Binbin Du, Jing Che, Huichao Shi, Haoqiang Zhao, Sheng Huang, Fenglin Deng, Zhenguo Shen, Mary Lou Guerinot, Luqing Zheng, Jian Feng Ma

PLANT JOURNAL 105 ( 3 ) 786 - 799 2021年2月

Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain

Gui Jie Lei, Naoki Yamaji, Jian Feng Ma

NEW PHYTOLOGIST 229 ( 2 ) 1007 - 1020 2021年1月

OsNRAMP1transporter contributes to cadmium and manganese uptake in rice

Jia-Dong Chang, Sheng Huang, Naoki Yamaji, Wenwen Zhang, Jian Feng Ma, Fang-Jie Zhao

PLANT CELL AND ENVIRONMENT 43 ( 10 ) 2476 - 2491 2020年10月

イネ種子の発育に必要な節局在性のリン酸輸送体

CHE Jing, CHE Jing, 山地直樹, 宮地孝明, 三谷奈見季, 加藤百合, SHEN Ren Fang, MA Jian Feng

日本植物生理学会年会(Web) 61 ( 8 ) 1387 - 1398 2020年8月

Breeding for low cadmium barley by introgression of a Sukkula-like transposable element

Gui Jie Lei, Miho Fujii-Kashino, De Zhi Wu, Hiroshi Hisano, Daisuke Saisho, Fenglin Deng, Naoki Yamaji, Kazuhiro Sato, Fang-Jie Zhao, Jian Feng Ma

Nature Food 1 ( 8 ) 489 - 499 2020年8月

The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions(1)([OPEN])

Sheng Huang, Akimasa Sasaki, Naoki Yamaji, Haruka Okada, Namiki Mitani-Ueno, Jian Feng Ma

PLANT PHYSIOLOGY 183 ( 3 ) 1224 - 1234 2020年7月

Diel magnesium fluctuations in chloroplasts contribute to photosynthesis in rice

Jian Li, Kengo Yokosho, Sheng Liu, Hong Rui Cao, Naoki Yamaji, Xin Guang Zhu, Hong Liao, Jian Feng Ma, Zhi Chang Chen

NATURE PLANTS 6 ( 7 ) 848 - + 2020年7月

Plastic transport systems of rice for mineral elements in response to diverse soil environmental changes

Peitong Wang, Naoki Yamaji, Komaki Inoue, Keiich Mochida, Jian Feng Ma

NEW PHYTOLOGIST 226 ( 1 ) 156 - 169 2020年4月

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