研究者詳細 - 山地　直樹

論文 - 山地　直樹

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Symplastic and apoplastic pathways for local distribution of silicon in rice leaves

Sheng Huang, Naoki Yamaji, Noriyuki Konishi, Namiki Mitani‐Ueno, Jian Feng Ma

New Phytologist 2025年8月

Dual roles of suberin deposition at endodermal Casparian strip in manganese uptake of rice

Toshiki Fujii, Naoki Yamaji, Jian Feng Ma

Journal of Experimental Botany 2025年7月

Shoot-Silicon-Signal protein to regulate root silicon uptake in rice 査読

Naoki Yamaji, Namiki Mitani-Ueno, Toshiki Fujii, Tomonori Shinya, Ji Feng Shao, Shota Watanuki, Yasunori Saitoh, Jian Feng Ma

Nature Communications 15 ( 1 ) 2024年12月

OsHAK4 functions in retrieving sodium from the phloem at the reproductive stage of rice. 国際誌

Jing Che, Naoki Yamaji, Shao Fei Wang, Yue Xia, Shun Ying Yang, Yan Hua Su, Ren Fang Shen, Jian Feng Ma

The Plant journal : for cell and molecular biology 120 ( 1 ) 76 - 90 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Soil salinity significantly limits rice productivity, but it is poorly understood how excess sodium (Na+) is delivered to the grains at the reproductive stage. Here, we functionally characterized OsHAK4, a member of the clade IV HAK/KUP/KT transporter subfamily in rice. OsHAK4 was localized to the plasma membrane and exhibited influx transport activity for Na+, but not for K+. Analysis of organ- and growth stage-dependent expression patterns showed that very low expression levels of OsHAK4 were detected at the vegetative growth stage, but its high expression in uppermost node I, peduncle, and rachis was found at the reproductive stage. Immunostaining indicated OsHAK4 localization in the phloem region of node I, peduncle, and rachis. Knockout of OsHAK4 did not affect the growth and Na+ accumulation at the vegetative stage. However, at the reproductive stage, the hak4 mutants accumulated higher Na+ in the peduncle, rachis, husk, and brown rice compared to the wild-type rice. Element imaging revealed higher Na+ accumulation at the phloem region of the peduncle in the mutants. These results indicate that OsHAK4 plays a crucial role in retrieving Na+ from the phloem in the upper nodes, peduncle, and rachis, thereby preventing Na+ distribution to the grains at the reproductive stage of rice.

DOI： 10.1111/tpj.16971

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Tissue-specific deposition, speciation and transport of antimony in rice

Hengliang Huang, Naoki Yamaji, Jian Feng Ma

Plant Physiology 2024年7月

An oligo peptide transporter family member, OsOPT7, mediates xylem unloading of Fe for its preferential distribution in rice

Naoki Yamaji, Yuma Yoshioka, Sheng Huang, Takaaki Miyaji, Akimasa Sasaki, Jian Feng Ma

New Phytologist 2024年6月

Metal Transport Systems in Plants. 国際誌

Sheng Huang, Naoki Yamaji, Jian Feng Ma

Annual review of plant biology 2024年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Plants take up metals, including the essential micronutrients [iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn)] and the toxic heavy metal cadmium (Cd), from soil and accumulate these metals in their edible parts, which are direct and indirect intake sources for humans. Multiple transporters belonging to different families are required to transport a metal from the soil to different organs and tissues, but only a few of them have been fully functionally characterized. The transport systems (the transporters required for uptake, translocation, distribution, redistribution, and their regulation) differ with metals and plant species, depending on the physiological roles, requirements of each metal, and anatomies of different organs and tissues. To maintain metal homeostasis in response to spatiotemporal fluctuations of metals in soil, plants have developed sophisticated and tightly regulated mechanisms through the regulation of transporters at the transcriptional and/or posttranscriptional levels. The manipulation of some transporters has succeeded in generating crops rich in essential metals but low in Cd accumulation. A better understanding of metal transport systems will contribute to better and safer crop production. Expected final online publication date for the Annual Review of Plant Biology, Volume 75 is May 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

DOI： 10.1146/annurev-arplant-062923-021424

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Uptake and Accumulation of Cobalt Is Mediated by OsNramp5 in Rice

Hengliang Huang, Naoki Yamaji, Sheng Huang, Jian Feng Ma

Plant Cell and Environment 2024年

Local distribution of manganese to leaf sheath is mediated by OsNramp5 in rice. 国際誌

Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Jian Feng Ma

The New phytologist 2023年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

To play essential roles of manganese (Mn) in plant growth and development, it needs to be transported to different organs and tissues after uptake. However, the molecular mechanisms underlying Mn distribution between different tissues are poorly understood. We functionally characterized a member of rice natural resistance-associated macrophage protein (NRAMP) family, OsNramp5 in terms of its tissue specificity of gene expression, cell-specificity of protein localization, phenotypic analysis of leaf growth and response to Mn fluctuations. OsNramp5 is highly expressed in the leaf sheath. Immunostaining revealed that OsNramp5 is polarly localized at the proximal side of xylem parenchyma cells of the leaf sheath. Both the gene expression and protein abundance of OsNramp5 are unaffected by different Mn concentrations. Knockout of OsNramp5 decreased the distribution of Mn to the leaf sheath, but increased the distribution to the leaf blade at both low and high Mn supplies, resulting in reduced growth of leaf sheath. Furthermore, expression of OsNramp5 under the control of root-specific promoter in osnramp5 mutant complemented Mn uptake, but could not complement Mn distribution to the leaf sheath. These results indicate that OsNramp5 expressed in the leaf sheath plays an important role in unloading Mn from the xylem for the local distribution in rice.

DOI： 10.1111/nph.19454

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Knockout of a rice K5.2 gene increases Ca accumulation in the grain. 国際誌

Peitong Wang, Naoki Yamaji, Namiki Mitani-Ueno, Jun Ge, Jian Feng Ma

Journal of integrative plant biology 2023年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Rice is a staple food for half world's population, but it is a poor dietary source of calcium (Ca) due to its low concentration. Therefore, it is an important issue to boost the Ca concentration in the grain for improving Ca deficiency risk, but the mechanisms underlying the Ca accumulation are poorly understood. Here, we obtained a rice (Oryza sativa) mutant with high shoot Ca accumulation. The mutant exhibited 26-53% higher Ca in the shoots than that in wild-type rice (WT) at different Ca supplies. The Ca concentration in the xylem sap was 36% higher in the mutant than in the WT. There was no difference in agronomic traits between the WT and mutant, but the mutant showed 25% higher Ca in the polished grain compared with the WT. Map-based cloning combined with complementation test revealed that the mutant phenotype was caused by an 18-bp deletion of a gene, OsK5.2 belonging to Shaker-like K+ channel family. OsK.2 was highly expressed in the mature region of the roots and its expression in the roots was not affected by Ca levels, but up-regulated by low K. Immunostaining showed that OsK.2 was mainly expressed in pericycle of the roots. Taken together, our results revealed a novel role of OsK.2 in Ca translocation in rice, which will be a good target for Ca biofortification in rice. This article is protected by copyright. All rights reserved.

DOI： 10.1111/jipb.13587

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A silicon transporter gene required for healthy growth of rice on land. 国際誌

Namiki Mitani-Ueno, Naoki Yamaji, Sheng Huang, Yuma Yoshioka, Takaaki Miyaji, Jian Feng Ma

Nature communications 14 ( 1 ) 6522 - 6522 2023年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Silicon (Si) is the most abundant mineral element in the earth's crust. Some plants actively accumulate Si as amorphous silica (phytoliths), which can protect plants from stresses. Here, we report a gene (SIET4) that is required for the proper accumulation and cell-specific deposition of Si in rice and show that it is essential for normal growth. SIET4 is constitutively expressed in leaves and encodes a Si transporter. SlET4 polarly localizes at the distal side of epidermal cells and cells surrounding the bulliform cells (motor cells) of the leaf blade, where Si is deposited. Knockout of SIET4 leads to the death of rice in the presence but not absence of Si. Further analysis shows that SIET4 knockout induces abnormal Si deposition in mesophyll cells and the induction of hundreds of genes related to various stress responses. These results indicate that SIET4 is required for the proper export of Si from leaf cells to the leaf surface and for the healthy growth of rice on land.

DOI： 10.1038/s41467-023-42180-y

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A vacuolar transporter plays important roles in zinc and cadmium accumulation in rice grain. 国際誌

Min Ning, Shi Jia Liu, Fenglin Deng, Liyu Huang, Hu Li, Jing Che, Naoki Yamaji, Fengyi Hu, Gui Jie Lei

The New phytologist 239 ( 5 ) 1919 - 1934 2023年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

Rice grain is a poor dietary source of zinc (Zn) but the primary source of cadmium (Cd) for humans; however, the molecular mechanisms for their accumulation in rice grain remain incompletely understood. This study functionally characterized a tonoplast-localized transporter, OsMTP1. OsMTP1 was preferentially expressed in the roots, aleurone layer, and embryo of seeds. OsMTP1 knockout decreased Zn concentration in the root cell sap, roots, aleurone layer and embryo, and subsequently increased Zn concentration in shoots and polished rice (endosperm) without yield penalty. OsMTP1 haplotype analysis revealed elite alleles associated with increased Zn level in polished rice, mostly because of the decreased OsMTP1 transcripts. OsMTP1 expression in yeast enhanced Zn tolerance but did not affect that of Cd. While OsMTP1 knockout resulted in decreased uptake, translocation and accumulation of Cd in plant and rice grain, which could be attributed to the indirect effects of altered Zn accumulation. Our results suggest that rice OsMTP1 primarily functions as a tonoplast-localized transporter for sequestrating Zn into vacuole. OsMTP1 knockout elevated Zn concentration but prevented Cd deposition in polished rice without yield penalty. Thus, OsMTP1 is a candidate gene for enhancing Zn level and reducing Cd level in rice grains.

DOI： 10.1111/nph.19070

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Polar localization of a rice silicon transporter requires isoleucine at both C- and N-termini as well as positively charged residues. 国際誌

Noriyuki Konishi, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

The Plant cell 35 ( 6 ) 2232 - 2250 2023年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

Silicon (Si) is important for stable and high yields in rice (Oryza sativa), a typical Si hyperaccumulator. The high Si accumulation is achieved by the cooperation of 2 Si transporters, LOW SILICON 1 (OsLsi1) and OsLsi2, which are polarly localized in cells of the root exodermis and endodermis. However, the mechanism underlying their polar localization is unknown. Here, we identified amino acid residues critical for the polar localization of OsLsi1. Deletion of both N- and C-terminal regions resulted in the loss of its polar localization. Furthermore, the deletion of the C-terminus inhibited its trafficking from the endoplasmic reticulum to the plasma membrane. Detailed site-directed mutagenesis analysis showed that Ile18 at the N-terminal region and Ile285 at the C-terminal region were essential for the polar localization of OsLsi1. Moreover, a cluster of positively charged residues at the C-terminal region is also required for polar localization. Phosphorylation and Lys modifications of OsLsi1 are unlikely to be involved in its polar localization. Finally, we showed that the polar localization of OsLsi1 is required for the efficient uptake of Si. Our study not only identified critical residues required for the polar localization of OsLsi1, but also provided experimental evidence for the importance of transporter polarity for efficient nutrient uptake.

DOI： 10.1093/plcell/koad073

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FE UPTAKE-INDUCING PEPTIDE1 maintains Fe translocation by controlling Fe deficiency response genes in the vascular tissue of Arabidopsis. 国際誌

Satoshi Okada, Gui J Lei, Naoki Yamaji, Sheng Huang, Jian F Ma, Keiichi Mochida, Takashi Hirayama

Plant, cell & environment 45 ( 11 ) 3322 - 3337 2022年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

FE UPTAKE-INDUCING PEPTIDE1 (FEP1), also named IRON MAN3 (IMA3) is a short peptide involved in the iron deficiency response in Arabidopsis thaliana. Recent studies uncovered its molecular function, but its physiological function in the systemic Fe response is not fully understood. To explore the physiological function of FEP1 in iron homoeostasis, we performed a transcriptome analysis using the FEP1 loss-of-function mutant fep1-1 and a transgenic line with oestrogen-inducible expression of FEP1. We determined that FEP1 specifically regulates several iron deficiency-responsive genes, indicating that FEP1 participates in iron translocation rather than iron uptake in roots. The iron concentration in xylem sap under iron-deficient conditions was lower in the fep1-1 mutant and higher in FEP1-induced transgenic plants compared with the wild type (WT). Perls staining revealed a greater accumulation of iron in the cortex of fep1-1 roots than in the WT root cortex, although total iron levels in roots were comparable in the two genotypes. Moreover, the fep1-1 mutation partially suppressed the iron overaccumulation phenotype in the leaves of the oligopeptide transporter3-2 (opt3-2) mutant. These data suggest that FEP1 plays a pivotal role in iron movement and in maintaining the iron quota in vascular tissues in Arabidopsis.

DOI： 10.1111/pce.14424

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Linking root morphology and anatomy with transporters for mineral element uptake in plants

Yu En, Naoki Yamaji, Jian Feng Ma

Plant and Soil 2022年9月

Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain

En Yu, Wenguang Wang, Naoki Yamaji, Shuichi Fukuoka, Jing Che, Daisei Ueno, Tsuyu Ando, Fenglin Deng, Kiyosumi Hori, Masahiro Yano, Ren Fang Shen, Jian Feng Ma

Nature Food 2022年8月

A Golgi‐localized glycosyltransferase, OsGT14 ;1 is required for growth of both roots and shoots in rice

Peitong Wang, Naoki Yamaji, Jian Feng Ma

The Plant Journal 2022年7月

NRAMP6 and NRAMP1 cooperatively regulate root growth and manganese translocation under manganese deficiency in Arabidopsis

Lun Li, Zongzheng Zhu, Yonghui Liao, Changhong Yang, Ni Fan, Jie Zhang, Naoki Yamaji, Léon Dirick, Jian Feng Ma, Catherine Curie, Chao‐Feng Huang

The Plant Journal 2022年6月

Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice

Noriyuki Konishi, Sheng Huang, Naoki Yamaji, Jian Feng Ma

Plant and Cell Physiology 63 ( 5 ) 699 - 712 2022年5月

A pericycle‐localized silicon transporter for efficient xylem loading in rice

Sheng Huang, Naoki Yamaji, Gen Sakurai, Namiki Mitani‐Ueno, Noriyuki Konishi, Jian Feng Ma

New Phytologist 234 ( 1 ) 197 - 208 2022年4月

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