研究者詳細 - 山地　直樹

論文 - 山地　直樹

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Symplastic and apoplastic pathways for local distribution of silicon in rice leaves

Sheng Huang, Naoki Yamaji, Noriyuki Konishi, Namiki Mitani‐Ueno, Jian Feng Ma

New Phytologist 2025年8月

Dual roles of suberin deposition at endodermal Casparian strip in manganese uptake of rice

Toshiki Fujii, Naoki Yamaji, Jian Feng Ma

Journal of Experimental Botany 2025年7月

Shoot-Silicon-Signal protein to regulate root silicon uptake in rice 査読

Naoki Yamaji, Namiki Mitani-Ueno, Toshiki Fujii, Tomonori Shinya, Ji Feng Shao, Shota Watanuki, Yasunori Saitoh, Jian Feng Ma

Nature Communications 15 ( 1 ) 2024年12月

OsHAK4 functions in retrieving sodium from the phloem at the reproductive stage of rice. 国際誌

Jing Che, Naoki Yamaji, Shao Fei Wang, Yue Xia, Shun Ying Yang, Yan Hua Su, Ren Fang Shen, Jian Feng Ma

The Plant journal : for cell and molecular biology 120 ( 1 ) 76 - 90 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Soil salinity significantly limits rice productivity, but it is poorly understood how excess sodium (Na+) is delivered to the grains at the reproductive stage. Here, we functionally characterized OsHAK4, a member of the clade IV HAK/KUP/KT transporter subfamily in rice. OsHAK4 was localized to the plasma membrane and exhibited influx transport activity for Na+, but not for K+. Analysis of organ- and growth stage-dependent expression patterns showed that very low expression levels of OsHAK4 were detected at the vegetative growth stage, but its high expression in uppermost node I, peduncle, and rachis was found at the reproductive stage. Immunostaining indicated OsHAK4 localization in the phloem region of node I, peduncle, and rachis. Knockout of OsHAK4 did not affect the growth and Na+ accumulation at the vegetative stage. However, at the reproductive stage, the hak4 mutants accumulated higher Na+ in the peduncle, rachis, husk, and brown rice compared to the wild-type rice. Element imaging revealed higher Na+ accumulation at the phloem region of the peduncle in the mutants. These results indicate that OsHAK4 plays a crucial role in retrieving Na+ from the phloem in the upper nodes, peduncle, and rachis, thereby preventing Na+ distribution to the grains at the reproductive stage of rice.

DOI： 10.1111/tpj.16971

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Tissue-specific deposition, speciation and transport of antimony in rice

Hengliang Huang, Naoki Yamaji, Jian Feng Ma

Plant Physiology 2024年7月

An oligo peptide transporter family member, OsOPT7, mediates xylem unloading of Fe for its preferential distribution in rice

Naoki Yamaji, Yuma Yoshioka, Sheng Huang, Takaaki Miyaji, Akimasa Sasaki, Jian Feng Ma

New Phytologist 2024年6月

Metal Transport Systems in Plants. 国際誌

Sheng Huang, Naoki Yamaji, Jian Feng Ma

Annual review of plant biology 2024年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Plants take up metals, including the essential micronutrients [iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn)] and the toxic heavy metal cadmium (Cd), from soil and accumulate these metals in their edible parts, which are direct and indirect intake sources for humans. Multiple transporters belonging to different families are required to transport a metal from the soil to different organs and tissues, but only a few of them have been fully functionally characterized. The transport systems (the transporters required for uptake, translocation, distribution, redistribution, and their regulation) differ with metals and plant species, depending on the physiological roles, requirements of each metal, and anatomies of different organs and tissues. To maintain metal homeostasis in response to spatiotemporal fluctuations of metals in soil, plants have developed sophisticated and tightly regulated mechanisms through the regulation of transporters at the transcriptional and/or posttranscriptional levels. The manipulation of some transporters has succeeded in generating crops rich in essential metals but low in Cd accumulation. A better understanding of metal transport systems will contribute to better and safer crop production. Expected final online publication date for the Annual Review of Plant Biology, Volume 75 is May 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

DOI： 10.1146/annurev-arplant-062923-021424

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Uptake and Accumulation of Cobalt Is Mediated by OsNramp5 in Rice

Hengliang Huang, Naoki Yamaji, Sheng Huang, Jian Feng Ma

Plant Cell and Environment 2024年

Local distribution of manganese to leaf sheath is mediated by OsNramp5 in rice. 国際誌

Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Jian Feng Ma

The New phytologist 2023年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

To play essential roles of manganese (Mn) in plant growth and development, it needs to be transported to different organs and tissues after uptake. However, the molecular mechanisms underlying Mn distribution between different tissues are poorly understood. We functionally characterized a member of rice natural resistance-associated macrophage protein (NRAMP) family, OsNramp5 in terms of its tissue specificity of gene expression, cell-specificity of protein localization, phenotypic analysis of leaf growth and response to Mn fluctuations. OsNramp5 is highly expressed in the leaf sheath. Immunostaining revealed that OsNramp5 is polarly localized at the proximal side of xylem parenchyma cells of the leaf sheath. Both the gene expression and protein abundance of OsNramp5 are unaffected by different Mn concentrations. Knockout of OsNramp5 decreased the distribution of Mn to the leaf sheath, but increased the distribution to the leaf blade at both low and high Mn supplies, resulting in reduced growth of leaf sheath. Furthermore, expression of OsNramp5 under the control of root-specific promoter in osnramp5 mutant complemented Mn uptake, but could not complement Mn distribution to the leaf sheath. These results indicate that OsNramp5 expressed in the leaf sheath plays an important role in unloading Mn from the xylem for the local distribution in rice.

DOI： 10.1111/nph.19454

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Knockout of a rice K5.2 gene increases Ca accumulation in the grain. 国際誌

Peitong Wang, Naoki Yamaji, Namiki Mitani-Ueno, Jun Ge, Jian Feng Ma

Journal of integrative plant biology 2023年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Rice is a staple food for half world's population, but it is a poor dietary source of calcium (Ca) due to its low concentration. Therefore, it is an important issue to boost the Ca concentration in the grain for improving Ca deficiency risk, but the mechanisms underlying the Ca accumulation are poorly understood. Here, we obtained a rice (Oryza sativa) mutant with high shoot Ca accumulation. The mutant exhibited 26-53% higher Ca in the shoots than that in wild-type rice (WT) at different Ca supplies. The Ca concentration in the xylem sap was 36% higher in the mutant than in the WT. There was no difference in agronomic traits between the WT and mutant, but the mutant showed 25% higher Ca in the polished grain compared with the WT. Map-based cloning combined with complementation test revealed that the mutant phenotype was caused by an 18-bp deletion of a gene, OsK5.2 belonging to Shaker-like K+ channel family. OsK.2 was highly expressed in the mature region of the roots and its expression in the roots was not affected by Ca levels, but up-regulated by low K. Immunostaining showed that OsK.2 was mainly expressed in pericycle of the roots. Taken together, our results revealed a novel role of OsK.2 in Ca translocation in rice, which will be a good target for Ca biofortification in rice. This article is protected by copyright. All rights reserved.

DOI： 10.1111/jipb.13587

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A silicon transporter gene required for healthy growth of rice on land. 国際誌

Namiki Mitani-Ueno, Naoki Yamaji, Sheng Huang, Yuma Yoshioka, Takaaki Miyaji, Jian Feng Ma

Nature communications 14 ( 1 ) 6522 - 6522 2023年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Silicon (Si) is the most abundant mineral element in the earth's crust. Some plants actively accumulate Si as amorphous silica (phytoliths), which can protect plants from stresses. Here, we report a gene (SIET4) that is required for the proper accumulation and cell-specific deposition of Si in rice and show that it is essential for normal growth. SIET4 is constitutively expressed in leaves and encodes a Si transporter. SlET4 polarly localizes at the distal side of epidermal cells and cells surrounding the bulliform cells (motor cells) of the leaf blade, where Si is deposited. Knockout of SIET4 leads to the death of rice in the presence but not absence of Si. Further analysis shows that SIET4 knockout induces abnormal Si deposition in mesophyll cells and the induction of hundreds of genes related to various stress responses. These results indicate that SIET4 is required for the proper export of Si from leaf cells to the leaf surface and for the healthy growth of rice on land.

DOI： 10.1038/s41467-023-42180-y

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A vacuolar transporter plays important roles in zinc and cadmium accumulation in rice grain. 国際誌

Min Ning, Shi Jia Liu, Fenglin Deng, Liyu Huang, Hu Li, Jing Che, Naoki Yamaji, Fengyi Hu, Gui Jie Lei

The New phytologist 239 ( 5 ) 1919 - 1934 2023年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

Rice grain is a poor dietary source of zinc (Zn) but the primary source of cadmium (Cd) for humans; however, the molecular mechanisms for their accumulation in rice grain remain incompletely understood. This study functionally characterized a tonoplast-localized transporter, OsMTP1. OsMTP1 was preferentially expressed in the roots, aleurone layer, and embryo of seeds. OsMTP1 knockout decreased Zn concentration in the root cell sap, roots, aleurone layer and embryo, and subsequently increased Zn concentration in shoots and polished rice (endosperm) without yield penalty. OsMTP1 haplotype analysis revealed elite alleles associated with increased Zn level in polished rice, mostly because of the decreased OsMTP1 transcripts. OsMTP1 expression in yeast enhanced Zn tolerance but did not affect that of Cd. While OsMTP1 knockout resulted in decreased uptake, translocation and accumulation of Cd in plant and rice grain, which could be attributed to the indirect effects of altered Zn accumulation. Our results suggest that rice OsMTP1 primarily functions as a tonoplast-localized transporter for sequestrating Zn into vacuole. OsMTP1 knockout elevated Zn concentration but prevented Cd deposition in polished rice without yield penalty. Thus, OsMTP1 is a candidate gene for enhancing Zn level and reducing Cd level in rice grains.

DOI： 10.1111/nph.19070

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Polar localization of a rice silicon transporter requires isoleucine at both C- and N-termini as well as positively charged residues. 国際誌

Noriyuki Konishi, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

The Plant cell 35 ( 6 ) 2232 - 2250 2023年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

Silicon (Si) is important for stable and high yields in rice (Oryza sativa), a typical Si hyperaccumulator. The high Si accumulation is achieved by the cooperation of 2 Si transporters, LOW SILICON 1 (OsLsi1) and OsLsi2, which are polarly localized in cells of the root exodermis and endodermis. However, the mechanism underlying their polar localization is unknown. Here, we identified amino acid residues critical for the polar localization of OsLsi1. Deletion of both N- and C-terminal regions resulted in the loss of its polar localization. Furthermore, the deletion of the C-terminus inhibited its trafficking from the endoplasmic reticulum to the plasma membrane. Detailed site-directed mutagenesis analysis showed that Ile18 at the N-terminal region and Ile285 at the C-terminal region were essential for the polar localization of OsLsi1. Moreover, a cluster of positively charged residues at the C-terminal region is also required for polar localization. Phosphorylation and Lys modifications of OsLsi1 are unlikely to be involved in its polar localization. Finally, we showed that the polar localization of OsLsi1 is required for the efficient uptake of Si. Our study not only identified critical residues required for the polar localization of OsLsi1, but also provided experimental evidence for the importance of transporter polarity for efficient nutrient uptake.

DOI： 10.1093/plcell/koad073

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FE UPTAKE-INDUCING PEPTIDE1 maintains Fe translocation by controlling Fe deficiency response genes in the vascular tissue of Arabidopsis. 国際誌

Satoshi Okada, Gui J Lei, Naoki Yamaji, Sheng Huang, Jian F Ma, Keiichi Mochida, Takashi Hirayama

Plant, cell & environment 45 ( 11 ) 3322 - 3337 2022年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

FE UPTAKE-INDUCING PEPTIDE1 (FEP1), also named IRON MAN3 (IMA3) is a short peptide involved in the iron deficiency response in Arabidopsis thaliana. Recent studies uncovered its molecular function, but its physiological function in the systemic Fe response is not fully understood. To explore the physiological function of FEP1 in iron homoeostasis, we performed a transcriptome analysis using the FEP1 loss-of-function mutant fep1-1 and a transgenic line with oestrogen-inducible expression of FEP1. We determined that FEP1 specifically regulates several iron deficiency-responsive genes, indicating that FEP1 participates in iron translocation rather than iron uptake in roots. The iron concentration in xylem sap under iron-deficient conditions was lower in the fep1-1 mutant and higher in FEP1-induced transgenic plants compared with the wild type (WT). Perls staining revealed a greater accumulation of iron in the cortex of fep1-1 roots than in the WT root cortex, although total iron levels in roots were comparable in the two genotypes. Moreover, the fep1-1 mutation partially suppressed the iron overaccumulation phenotype in the leaves of the oligopeptide transporter3-2 (opt3-2) mutant. These data suggest that FEP1 plays a pivotal role in iron movement and in maintaining the iron quota in vascular tissues in Arabidopsis.

DOI： 10.1111/pce.14424

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Linking root morphology and anatomy with transporters for mineral element uptake in plants

Yu En, Naoki Yamaji, Jian Feng Ma

Plant and Soil 2022年9月

Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain

En Yu, Wenguang Wang, Naoki Yamaji, Shuichi Fukuoka, Jing Che, Daisei Ueno, Tsuyu Ando, Fenglin Deng, Kiyosumi Hori, Masahiro Yano, Ren Fang Shen, Jian Feng Ma

Nature Food 2022年8月

A Golgi‐localized glycosyltransferase, OsGT14 ;1 is required for growth of both roots and shoots in rice

Peitong Wang, Naoki Yamaji, Jian Feng Ma

The Plant Journal 2022年7月

NRAMP6 and NRAMP1 cooperatively regulate root growth and manganese translocation under manganese deficiency in Arabidopsis

Lun Li, Zongzheng Zhu, Yonghui Liao, Changhong Yang, Ni Fan, Jie Zhang, Naoki Yamaji, Léon Dirick, Jian Feng Ma, Catherine Curie, Chao‐Feng Huang

The Plant Journal 2022年6月

Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice

Noriyuki Konishi, Sheng Huang, Naoki Yamaji, Jian Feng Ma

Plant and Cell Physiology 63 ( 5 ) 699 - 712 2022年5月

A pericycle‐localized silicon transporter for efficient xylem loading in rice

Sheng Huang, Naoki Yamaji, Gen Sakurai, Namiki Mitani‐Ueno, Noriyuki Konishi, Jian Feng Ma

New Phytologist 234 ( 1 ) 197 - 208 2022年4月

Effects of polygalacturonase overexpression on pectin distribution in the elongation zones of roots under aluminium stress. 国際誌

Teruki Nagayama, Akane Tatsumi, Atsuko Nakamura, Naoki Yamaji, Shinobu Satoh, Jun Furukawa, Hiroaki Iwai

AoB PLANTS 14 ( 2 ) plac003 2022年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

The roots of many plant species contain large amounts of pectin and it contributes to the formation of the rhizosphere. In the present study, the relationship between the root-tip pectin content and aluminium (Al) tolerance in wild-type (WT) and demethylesterified pectin degradation enzyme gene overexpressor (OsPG2-FOX) rice lines was compared. OsPG2-FOX rice showed reduced pectin content in roots, even under control conditions; Al treatment reduced root elongation and the pectin content in the root elongation zone. Wild-type rice showed more pectin accumulation in the root elongation zone after Al treatment. Relative to WT rice, OsPG2-FOX rice showed more Al accumulation in the root elongation zone. These results indicate that the amount of pectin influences Al tolerance and that the distribution of pectin in the root elongation zone inhibits Al accumulation in rice roots. Pectin accumulation in cell walls in the root elongation zone may play a role in protecting rice plants from the Al-induced inhibition of root elongation by regulating pectin distribution.

DOI： 10.1093/aobpla/plac003

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A crucial role for a node-localized transporter, HvSPDT, in loading phosphorus into barley grains. 国際誌

Mian Gu, Hengliang Huang, Hiroshi Hisano, Guangda Ding, Sheng Huang, Namiki Mitani-Ueno, Kengo Yokosho, Kazuhiro Sato, Naoki Yamaji, Jian Feng Ma

The New phytologist 2022年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Grains are the major sink of phosphorus (P) in cereal crops, accounting for 60-85% of total plant P, but the mechanisms underlying P loading into the grains are poorly understood. We functionally characterized a transporter gene required for the distribution of P to the grains in barley (Hordeum vulgare), HvSPDT (SULTR-like phosphorus distribution transporter). HvSPDT encoded a plasma membrane-localized Pi/H+ cotransporter. It was mainly expressed in the nodes at both the vegetative and reproductive stages. Furthermore, its expression was induced by inorganic phosphate (Pi) deficiency. In the nodes, HvSPDT was expressed in both the xylem and phloem region of enlarged and diffuse vascular bundles. Knockout of HvSPDT decreased the distribution of P to new leaves, but increased the distribution to old leaves at the vegetative growth stage under low P supply. However, knockout of HvSPDT did not alter the redistribution of P from old to young organs. At the reproductive stage, knockout of HvSPDT significantly decreased P allocation to the grains, resulting in a considerable reduction in grain yield, especially under P-limited conditions. Our results indicate that node-based HvSPDT plays a crucial role in loading P into barley grains through preferentially distributing P from the xylem and further to the phloem.

DOI： 10.1111/nph.18057

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Boron uptake in rice is regulated post-translationally via a clathrin-independent pathway

Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Ji Feng Shao, Namiki Mitani-Ueno, Jian Feng Ma

Plant Physiology 188 ( 3 ) 1649 - 1664 2021年12月

掲載種別：研究論文（学術雑誌）出版者・発行元：Oxford University Press (OUP)

<title>Abstract</title>
Uptake of boron (B) in rice (Oryza sativa) is mediated by the Low silicon rice 1 (OsLsi1) channel, belonging to the NOD26-like intrinsic protein III subgroup, and the efflux transporter B transporter 1 (OsBOR1). However, it is unknown how these transporters cooperate for B uptake and how they are regulated in response to B fluctuations. Here, we examined the response of these two transporters to environmental B changes at the transcriptional and posttranslational level. OsBOR1 showed polar localization at the proximal side of both the exodermis and endodermis of mature root region, forming an efficient uptake system with OsLsi1 polarly localized at the distal side of the same cell layers. Expression of OsBOR1 and OsLsi1 was unaffected by B deficiency and excess. However, although OsLsi1 protein did not respond to high B at the protein level, OsBOR1 was degraded in response to high B within hours, which was accompanied with a significant decrease of total B uptake. The high B-induced degradation of OsBOR1 was inhibited in the presence of MG-132, a proteasome inhibitor, without disturbance of the polar localization. In contrast, neither the high B-induced degradation of OsBOR1 nor its polarity was affected by induced expression of dominant-negative mutated dynamin-related protein 1A (OsDRP1AK47A) or knockout of the mu subunit (AP2M) of adaptor protein-2 complex, suggesting that clathrin-mediated endocytosis is not involved in OsBOR1 degradation and polar localization. These results indicate that, in contrast to Arabidopsis thaliana, rice has a distinct regulatory mechanism for B uptake through clathrin-independent degradation of OsBOR1 in response to high B.

DOI： 10.1093/plphys/kiab575

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Metalloid transporters and their regulation in plants

Naoki Yamaji, Jian Feng Ma

Plant Physiology 187 ( 4 ) 1929 - 1939 2021年12月

Zinc Transport in Rice: Dilemma between optimal plant requirement and human nutrition. 国際誌

Sheng Huang, Naoki Yamaji, Jian Feng Ma

Journal of experimental botany 2021年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Zinc (Zn) is an essential micronutrient for both plants and animals, while Zn deficiency in crops and humans is a global problem affecting crop productivity and human health. Since plants and humans differ in their Zn requirement, there is a dilemma between plant nutrition and human nutrition. In this review, we focus on the transport system of Zn from soil to grain in rice (Oryza sativa), which is a major dietary source of Zn for people subsiding on rice-based diets. We describe transporters belonging to different family, which are involved in the uptake, vacuolar sequestration, root-to-shoot translocation and distribution of Zn. We also discuss the possible regulation mechanism of these transporters. Several examples are given on enhancing Zn accumulation and bioavailability in rice grains through manipulation of genes highly expressed in the nodes, where Zn is highly deposited. We finally provide our perspectives toward breeding rice cultivars with both increased tolerance to Zn-deficiency stress and high Zn density in rice grains.

DOI： 10.1093/jxb/erab478

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Structural basis for high selectivity of a rice silicon channel Lsi1. 国際誌

Yasunori Saitoh, Namiki Mitani-Ueno, Keisuke Saito, Kengo Matsuki, Sheng Huang, Lingli Yang, Naoki Yamaji, Hiroshi Ishikita, Jian-Ren Shen, Jian Feng Ma, Michihiro Suga

Nature communications 12 ( 1 ) 6236 - 6236 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Silicon (Si), the most abundant mineral element in the earth's crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids.

DOI： 10.1038/s41467-021-26535-x

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Lateral roots but not root hairs contribute to high uptake of manganese and cadmium in rice. 国際誌

En Yu, Naoki Yamaji, Chuanzao Mao, Hua Wang, Jian Feng Ma

Journal of experimental botany 72 ( 20 ) 7219 - 7228 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Rice (Oryza sativa L.) can accumulate high manganese (Mn) in the shoots through uptake by the roots, which consist of crown roots, lateral roots and root hairs. We investigated the role of lateral roots and root hairs in Mn and cadmium (Cd) uptake by using two indica rice mutants defective in formation of lateral roots (osiaa11) and root hairs (osrhl1). The uptake of Mn and Cd in osiaa11 was significantly lower than that in wild type 'Kasalath', but there was no difference between wild type and osrhl1. Furthermore, a kinetic study showed that Mn uptake in osiaa11 was much lower than that in wild type and osrhl1 across a wide range of Mn concentrations. The role of lateral roots in Mn and Cd uptake was further confirmed in a japonica rice mutant defective in lateral root formation. We found that expression of Mn transporter gene Natural Resistance-Associated Macrophage Protein 5 (OsNRAMP5), but not of Metal Tolerance Protein 9 (OsMTP9), was lower in osiaa11 than in wild type; however, there were no differences between osrhl1 and the wild type. Immunostaining showed that OsNRAMP5 and OsMTP9 were localized in the exodermis and endodermis of crown roots and lateral roots, but not in the root hairs. Taken together, our results indicate that lateral roots, but not root hairs, play an important role in high Mn and Cd uptake in rice.

DOI： 10.1093/jxb/erab329

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Effectiveness of Create ML in microscopy image classifications: a simple and inexpensive deep learning pipeline for non-data scientists. 国際誌

Kiyotaka Nagaki, Tomoyuki Furuta, Naoki Yamaji, Daichi Kuniyoshi, Megumi Ishihara, Yuji Kishima, Minoru Murata, Atsushi Hoshino, Hirotomo Takatsuka

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 29 ( 3-4 ) 361 - 371 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Observing chromosomes is a time-consuming and labor-intensive process, and chromosomes have been analyzed manually for many years. In the last decade, automated acquisition systems for microscopic images have advanced dramatically due to advances in their controlling computer systems, and nowadays, it is possible to automatically acquire sets of tiling-images consisting of large number, more than 1000, of images from large areas of specimens. However, there has been no simple and inexpensive system to efficiently select images containing mitotic cells among these images. In this paper, a classification system of chromosomal images by deep learning artificial intelligence (AI) that can be easily handled by non-data scientists was applied. With this system, models suitable for our own samples could be easily built on a Macintosh computer with Create ML. As examples, models constructed by learning using chromosome images derived from various plant species were able to classify images containing mitotic cells among samples from plant species not used for learning in addition to samples from the species used. The system also worked for cells in tissue sections and tetrads. Since this system is inexpensive and can be easily trained via deep learning using scientists' own samples, it can be used not only for chromosomal image analysis but also for analysis of other biology-related images.

DOI： 10.1007/s10577-021-09676-z

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Buckwheat FeNramp5 Mediates High Manganese Uptake in Roots.

Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

Plant & cell physiology 62 ( 4 ) 600 - 609 2021年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

Manganese (Mn) is an essential element for plant growth and development, but transporters required for Mn uptake have only been identified in a few plant species. Here, we functionally characterized a member of the natural resistance-associated macrophage proteins (Nramps) family, FeNramp5 in buckwheat (Fagopyrum esculentum Moench), which is known as a species well adapted to acidic soils. FeNramp5 was mainly expressed in the roots, and its expression was upregulated by the deficiency of Mn and Fe. Furthermore, spatial and tissue-specific expression analysis showed that FeNramp5 was expressed in all tissues of the basal root regions. FeNramp5-GFP protein was localized to the plasma membrane when transiently expressed in buckwheat leaf protoplast. FeNramp5 showed the transport activity for Mn2+ and Cd2+ but not for Fe2+ when expressed in yeast. Furthermore, the transport activity for Mn2+ was higher in yeast expressing FeNramp5 than in yeast expressing AtNramp1. FeNramp5 was also able to complement the phenotype of Arabidopsis atnramp1 mutant in terms of the growth and accumulation of Mn and Cd. The absolute expression level of AtNramp1 was comparable to that of FeNramp5 in the roots, but buckwheat accumulated higher Mn than Arabidopsis when grown under the same condition. Further analysis showed that at least motif B in FeNramp5 seems important for its high transport activity for Mn. These results indicate that FeNramp5 is a transporter for the uptake of Mn and Cd and its higher transport activity for Mn is probably associated with higher Mn accumulation in buckwheat.

DOI： 10.1093/pcp/pcaa153

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Lysine Ketoglutarate Reductase Trans-Splicing Related 1 Is Involved In A Temperature-Dependent Root Growth in Rice. 国際誌

En Yu, Naoki Yamaji, Keiich Mochida, Ivan Galis, Kanatani Asaka, Jian Feng Ma

Journal of experimental botany 72 ( 18 ) 6336 - 6349 2021年5月

Role of a vacuolar iron transporter OsVIT2 in the distribution of iron to rice grains

Jing Che, Naoki Yamaji, Jian Feng Ma

NEW PHYTOLOGIST 230 ( 3 ) 1049 - 1062 2021年5月

Fine regulation system for distribution of boron to different tissues in rice 国際誌

Ji Feng Shao, Naoki Yamaji, Sheng Huang, Jian Feng Ma

NEW PHYTOLOGIST 230 ( 2 ) 656 - 668 2021年4月

A transporter for delivering zinc to the developing tiller bud and panicle in rice

Shuai Mu, Naoki Yamaji, Akimasa Sasaki, Le Luo, Binbin Du, Jing Che, Huichao Shi, Haoqiang Zhao, Sheng Huang, Fenglin Deng, Zhenguo Shen, Mary Lou Guerinot, Luqing Zheng, Jian Feng Ma

PLANT JOURNAL 105 ( 3 ) 786 - 799 2021年2月

Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain

Gui Jie Lei, Naoki Yamaji, Jian Feng Ma

NEW PHYTOLOGIST 229 ( 2 ) 1007 - 1020 2021年1月

OsNRAMP1transporter contributes to cadmium and manganese uptake in rice

Jia-Dong Chang, Sheng Huang, Naoki Yamaji, Wenwen Zhang, Jian Feng Ma, Fang-Jie Zhao

PLANT CELL AND ENVIRONMENT 43 ( 10 ) 2476 - 2491 2020年10月

イネ種子の発育に必要な節局在性のリン酸輸送体

CHE Jing, CHE Jing, 山地直樹, 宮地孝明, 三谷奈見季, 加藤百合, SHEN Ren Fang, MA Jian Feng

日本植物生理学会年会(Web) 61 ( 8 ) 1387 - 1398 2020年8月

Breeding for low cadmium barley by introgression of a Sukkula-like transposable element

Gui Jie Lei, Miho Fujii-Kashino, De Zhi Wu, Hiroshi Hisano, Daisuke Saisho, Fenglin Deng, Naoki Yamaji, Kazuhiro Sato, Fang-Jie Zhao, Jian Feng Ma

Nature Food 1 ( 8 ) 489 - 499 2020年8月

The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions(1)([OPEN])

Sheng Huang, Akimasa Sasaki, Naoki Yamaji, Haruka Okada, Namiki Mitani-Ueno, Jian Feng Ma

PLANT PHYSIOLOGY 183 ( 3 ) 1224 - 1234 2020年7月

Diel magnesium fluctuations in chloroplasts contribute to photosynthesis in rice

Jian Li, Kengo Yokosho, Sheng Liu, Hong Rui Cao, Naoki Yamaji, Xin Guang Zhu, Hong Liao, Jian Feng Ma, Zhi Chang Chen

NATURE PLANTS 6 ( 7 ) 848 - + 2020年7月

Plastic transport systems of rice for mineral elements in response to diverse soil environmental changes

Peitong Wang, Naoki Yamaji, Komaki Inoue, Keiich Mochida, Jian Feng Ma

NEW PHYTOLOGIST 226 ( 1 ) 156 - 169 2020年4月

Altered Root Structure Affects Both Expression and Cellular Localization of Transporters for Mineral Element Uptake in Rice

En Yu, Naoki Yamaji, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 61 ( 3 ) 481 - 491 2020年3月

Decrosslinking enables visualization of RNA-guided endonuclease?in situ labeling signals for DNA sequences in plant tissues. 査読

Kiyotaka Nagaki, Naoki Yamaji

Journal of Experimental Botany 71 ( 6 ) 1792 - 1800 2020年3月

A transcription factor OsbHLH156 regulates Strategy II iron acquisition through localising IRO2 to the nucleus in rice

Shoudong Wang, Lin Li, Yinghui Ying, Jin Wang, Ji Feng Shao, Naoki Yamaji, James Whelan, Jian Feng Ma, Huixia Shou

NEW PHYTOLOGIST 225 ( 3 ) 1247 - 1260 2020年2月

Vascular Cambium-Localized AtSPDT Mediates Xylem-to-Phloem Transfer of Phosphorus for Its Preferential Distribution in Arabidopsis

Guangda Ding, Gui Jie Lei, Naoki Yamaji, Kengo Yokosho, Namiki Mitani-Ueno, Sheng Huang, Jian Feng Ma

MOLECULAR PLANT 13 ( 1 ) 99 - 111 2020年1月

OsCASP1 Is Required for Casparian Strip Formation at Endodermal Cells of Rice Roots for Selective Uptake of Mineral Elements

Zhigang Wang, Naoki Yamaji, Sheng Huang, Xiang Zhang, Mingxing Shi, Shan Fu, Guangzhe Yang, Jian Feng, Jixing Xia

PLANT CELL 31 ( 11 ) 2636 - 2648 2019年11月

Changes in the Distribution of Pectin in Root Border Cells Under Aluminum Stress

Teruki Nagayama, Atsuko Nakamura, Naoki Yamaji, Shinobu Satoh, Jun Furukawa, Hiroaki Iwai

Frontiers in Plant Science 10 2019年10月

A Vacuolar Phytosiderophore Transporter Alters Iron and Zinc Accumulation in Polished Rice Grains

Jing Che, Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

PLANT PHYSIOLOGY 181 ( 1 ) 276 - 288 2019年9月

Bioimaging of multiple elements by high‐resolution LA ‐ ICP ‐ MS reveals altered distribution of mineral elements in the nodes of rice mutants 査読

Naoki Yamaji, Jian Feng Ma

The Plant Journal 99 ( 6 ) 1254 - 1263 2019年6月

The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice

Hongmei Cai, Sheng Huang, Jing Che, Naoki Yamaji, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 70 ( 10 ) 2717 - 2725 2019年5月

A nodule‐localized phosphate transporter Gm PT 7 plays an important role in enhancing symbiotic N 2 fixation and yield in soybean 査読

Liyu Chen, Lu Qin, Lili Zhou, Xinxin Li, Zhichang Chen, Lili Sun, Wenfei Wang, Zhihao Lin, Jing Zhao, Naoki Yamaji, Jian Feng Ma, Mian Gu, Guohua Xu, Hong Liao

New Phytologist 221 ( 4 ) 2013 - 2025 2019年3月

OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes 査読

Ming Xing Chang, Mian Gu, Yu Wei Xia, Xiao Li Dai, Chang Rong Dai, Jun Zhang, Shi Chao Wang, Hong Ye Qu, Naoki Yamaji, Jian Feng Ma, Guo Hua Xu

Plant Physiology 179 ( 2 ) 656 - 670 2019年2月

Retrotransposon Insertion and DNA Methylation Regulate Aluminum Tolerance in European Barley Accessions. 査読国際誌

Miho Kashino-Fujii, Kengo Yokosho, Naoki Yamaji, Miki Yamane, Daisuke Saisho, Kazuhiro Sato, Jian Feng Ma

Plant physiology 178 ( 2 ) 716 - 727 2018年10月

記述言語：英語

Aluminum (Al) toxicity is a major stress factor limiting crop productivity in acid soil. Although there is great genotypic variation in tolerance to Al toxicity, the underlying molecular mechanisms are poorly understood. Here, we report that, in barley (Hordeum vulgare), the fourth largest cereal crop produced in the world, both retrotransposon insertion and DNA methylation are involved in regulating differential Al tolerance. HvAACT1 is a major gene responsible for citrate secretion from the roots for external detoxification of Al. A multiretrotransposon-like (MRL) sequence insertion at least 15.3 kb in length was detected in the upstream genomic region of HvAACT1 that displayed promoter activity and significantly enhanced HvAACT1 expression, especially in the root tips of Al-tolerant accessions. Furthermore, in a number of accessions with low levels of HvAACT1 expression, this MRL insertion was present but highly methylated. Geographical analysis showed that accessions with this MRL insertion are distributed mainly in European areas with acid soils. Two wild barley accessions were found to possess this MRL insertion, but with a high degree of methylation. These results indicate that the MRL insertion and its degree of DNA methylation influence HvAACT1 expression and that demethylation of this MRL insertion, which facilitates adaptation to acid soils, occurred following barley domestication. Moreover, our results indicate that barley accessions in East Asia and Europe have developed independent but equivalent strategies to withstand Al toxicity in acid soils.

DOI： 10.1104/pp.18.00651

PubMed

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Engineering rice with lower grain arsenic

Fenglin Deng, Naoki Yamaji, Jian Feng Ma, Sang-Kyu Lee, Jong-Seong Jeon, Enrico Martinoia, Youngsook Lee, Won-Yong Song

PLANT BIOTECHNOLOGY JOURNAL 16 ( 10 ) 1691 - 1699 2018年10月

Two genes encoding a bacterial-type ATP-binding cassette transporter are implicated in aluminum tolerance in buckwheat. 査読

Che J, Yamaji N, Yokosho K, Shen RF, Ma JF

Plant & cell physiology 59 ( 12 ) 2502 - 2511 2018年8月

Efficient and flexible uptake system for mineral elements in plants 査読

Jing Che, Naoki Yamaji, Jian Feng Ma

New Phytologist 219 ( 2 ) 513 - 517 2018年7月

A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice 査読

Yuta Tsunemitsu, Mayuko Genga, Tomoyuki Okada, Naoki Yamaji, Jian Feng Ma, Akira Miyazaki, Shin ichiro Kato, Kozo Iwasaki, Daisei Ueno

Planta 248 ( 1 ) 231 - 241 2018年7月

The putative peptide gene FEP1 regulates iron deficiency response in Arabidopsis. 査読

Hirayama T, Lei GJ, Yamaji N, Nakagawa N, Ma JF

Plant & cell physiology 59 ( 9 ) 1739 - 1752 2018年7月

Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of the OsHMA2 promoter 査読

Ji Feng Shao, Jixing Xia, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

Journal of Experimental Botany 69 ( 10 ) 2743 - 2752 2018年4月

Transport system of mineral elements in rice 査読

Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

Rice Genomics, Genetics and Breeding 223 - 240 2018年3月

OsNRT2.4 encodes a dual-affinity nitrate transporter and functions in nitrate-regulated root growth and nitrate distribution in rice 査読

Jia Wei, Yi Zheng, Huimin Feng, Hongye Qu, Xiaorong Fan, Naoki Yamaji, Jian Feng Ma, Guohua Xu

Journal of Experimental Botany 69 ( 5 ) 1095 - 1107 2018年2月

Preferential distribution of boron to developing tissues is mediated by the intrinsic protein OSNIP3 査読

Ji Feng Shao, Naoki Yamaji, Xin Wei Liu, Kengo Yokosho, Ren Fang Shen, Jian Feng Ma

Plant Physiology 176 ( 2 ) 1739 - 1750 2018年2月

Rice reduces Mn uptake in response to Mn stress 査読

Yuta Tsunemitsu, Naoki Yamaji, Jian Feng Ma, Shin ichiro Kato, Kozo Iwasaki, Daisei Ueno

Plant Signaling and Behavior 13 ( 1 ) 2018年1月

Functional characterization of an aluminum (Al)-inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice 査読

Che Jing, Tsutsui Tomokazu, Yokosho Kengo, Yamaji Naoki, Ma Jian Feng

New Phytologist 220 ( 1 ) 209 - 218 2018年

Both retrotransposon insertion and demethylation regulate aluminum tolerance of European barley for postdomestication expansion to acid soil.

Fujii-Kashino, M, Yamaji, N, Yamane, M, Saisho, D, Sato, K, Ma, J. F

Plant Physiology 2018年

Two MATE Transporters with Different Subcellular Localization are Involved in Al Tolerance in Buckwheat 査読

Gui Jie Lei, Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 58 ( 12 ) 2179 - 2189 2017年12月

Node-controlled allocation of mineral elements in Poaceae 査読

Naoki Yamaji, Jian Feng Ma

CURRENT OPINION IN PLANT BIOLOGY 39 18 - 24 2017年10月

The tonoplast-localized transporter MTP8.2 contributes to manganese detoxification in the shoots and roots of oryza sativa L. 査読

Yuma Takemoto, Yuta Tsunemitsu, Miho Fujii-Kashino, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma, Shin Ichiro Kato, Kozo Iwasaki, Daisei Ueno

Plant and Cell Physiology 58 ( 9 ) 1573 - 1582 2017年9月

Silicon reduces cadmium accumulation by suppressing expression of transporter genes involved in cadmium uptake and translocation in rice 査読

Ji Feng Shao, Jing Che, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 68 ( 20 ) 5641 - 5651 2017年9月

Functional characterization of two half-size ABC transporter genes in aluminium-accumulating buckwheat 査読

Gui Jie Lei, Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

NEW PHYTOLOGIST 215 ( 3 ) 1080 - 1089 2017年8月

OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice 査読

Natsuko I. Kobayashi, Naoki Yamaji, Hiroki Yamamoto, Kaoru Okubo, Hiroki Ueno, Alex Costa, Keitaro Tanoi, Hideo Matsumura, Miho Fujii-Kashino, Tomoki Horiuchi, Mohammad Al Nayef, Sergey Shabala, Gynheung An, Jian Feng Ma, Tomoaki Horie

PLANT JOURNAL 91 ( 4 ) 657 - 670 2017年8月

A Magnesium Transporter OsMGT1 Plays a Critical Role in Salt Tolerance in Rice(1[OPEN]) 査読

Zhi Chang Chen, Naoki Yamaji, Tomoaki Horie, Jing Che, Jian Li, Gynheung An, Jian Feng Ma

PLANT PHYSIOLOGY 174 ( 3 ) 1837 - 1849 2017年7月

A Model of Silicon Dynamics in Rice: An Analysis of the Investment Efficiency of Si Transporters 査読

Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

FRONTIERS IN PLANT SCIENCE 8 2017年7月

The Key to Vn Homeostasis in Plants: Regulation of Mn Transporters 査読

Ji Feng Shao, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

TRENDS IN PLANT SCIENCE 22 ( 3 ) 215 - 224 2017年3月

ePro-ClearSee: a simple immunohistochemical method that does not require sectioning of plant samples 査読

Kiyotaka Nagaki, Naoki Yamaji, Minoru Murata

SCIENTIFIC REPORTS 7 2017年2月

Reducing phosphorus accumulation in rice grains with an impaired transporter in the node 査読

Naoki Yamaji, Yuma Takemoto, Takaaki Miyaji, Namiki Mitani-Ueno, Kaoru T. Y. Oshida, Jian Feng Ma

NATURE 541 ( 7635 ) 92 - + 2017年1月

Isolation and characterization of a rice line with high Cd accumulation for potential use in phytoremediation 査読

Ji Feng Shao, Miho Fujii-Kashino, Naoki Yamaji, Shinichi Fukuoka, Ren Fang Shen, Jian Feng Ma

PLANT AND SOIL 410 ( 1-2 ) 357 - 368 2017年1月

Reducing phosphorus accumulation in rice grains with an impaired transporter in the node (vol 541, pg 92, 2017) 査読

Naoki Yamaji, Yuma Takemoto, Takaaki Miyaji, Namiki Mitani-Ueno, Kaoru T. Yoshida, Jian Feng Ma

NATURE 541 ( 7635 ) 136 - 136 2017年1月

A model of silicon dynamics in rice 査読

Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

Frontiers in Plant Science 8 E31-E34 2017年

Retrotransposon-Mediated Aluminum Tolerance through Enhanced Expression of the Citrate Transporter OsFRDL4 査読

Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

PLANT PHYSIOLOGY 172 ( 4 ) 2327 - 2336 2016年12月

Analysis of epigenetic modifications in plant tissues by a novel clearing method 査読

Kiyotaka Nagaki, Naoki Yamaji, Minoru Murata

GENES & GENETIC SYSTEMS 91 ( 6 ) 346 - 346 2016年12月

High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice 査読

Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 57 ( 12 ) 2510 - 2518 2016年12月

The HvNramp5 Transporter Mediates Uptake of Cadmium and Manganese, But Not Iron. 査読国際誌

Dezhi Wu, Naoki Yamaji, Miki Yamane, Miho Kashino-Fujii, Kazuhiro Sato, Jian Feng Ma

Plant physiology 172 ( 3 ) 1899 - 1910 2016年11月

An Al-inducible expansin gene, OsEXPA10 is involved in root cell elongation of rice 査読

Jing Che, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

PLANT JOURNAL 88 ( 1 ) 132 - 142 2016年10月

OsFRDL1 expressed in nodes is required for distribution of iron to grains in rice 査読

Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 67 ( 18 ) 5485 - 5494 2016年10月

A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain 査読

Xin-Yuan Huang, Fenglin Deng, Naoki Yamaji, Shannon R. M. Pinson, Miho Fujii-Kashino, John Danku, Alex Douglas, Mary Lou Guerinot, David E. Salt, Jian Feng Ma

NATURE COMMUNICATIONS 7 2016年7月

Transporters involved in mineral nutrient uptake in rice 査読

Akimasa Sasaki, Naoki Yamaji, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 67 ( 12 ) 3645 - 3653 2016年6月

Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice 査読

Yosuke Toda, Yin Wang, Akira Takahashi, Yuya Kawai, Yasuomi Tada, Naoki Yamaji, Jian Feng Ma, Motoyuki Ashikari, Toshinori Kinoshita

PLANT AND CELL PHYSIOLOGY 57 ( 6 ) 1220 - 1230 2016年6月

An Aluminum-Inducible IREG Gene is Required for Internal Detoxification of Aluminum in Buckwheat 査読

Kengo Yokosho, Naoki Yamaji, Namiki Mitani-Ueno, Ren Fang Shen, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 57 ( 6 ) 1169 - 1178 2016年6月

A Cation-Chloride Cotransporter Gene Is Required for Cell Elongation and Osmoregulation in Rice 査読

Zhi Chang Chen, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

PLANT PHYSIOLOGY 171 ( 1 ) 494 - 507 2016年5月

Functional Analysis of a MATE Gene OsFRDL2 Revealed its Involvement in Al-Induced Secretion of Citrate, but a Lower Contribution to Al Tolerance in Rice 査読

Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 57 ( 5 ) 976 - 985 2016年5月

Silicon decreases both uptake and root-to-shoot translocation of manganese in rice 査読

Jing Che, Naoki Yamaji, Ji Feng Shao, Jian Feng Ma, Ren Fang Shen

JOURNAL OF EXPERIMENTAL BOTANY 67 ( 5 ) 1535 - 1544 2016年3月

OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress 査読

Kei Suzuki, Naoki Yamaji, Alex Costa, Eiji Okuma, Natsuko I. Kobayashi, Tatsuhiko Kashiwagi, Maki Katsuhara, Cun Wang, Keitaro Tanoi, Yoshiyuki Murata, Julian I. Schroeder, Jian Feng Ma, Tomoaki Horie

BMC PLANT BIOLOGY 16 2016年1月

4-3-8 Two genes encoding bacterial-type ABC transporters are required for Al tolerance in buckwheat（4-3 植物の有害元素，2016年度佐賀大会）

車景, 横正健剛, 山地直樹, 馬建鋒

日本土壌肥料学会講演要旨集 62 65 - 65 2016年

A polarly localized transporter for efficient manganese uptake in rice 査読

Daisei Ueno, Akimasa Sasaki, Naoki Yamaji, Takaaki Miyaji, Yumi Fujii, Yuma Takemoto, Sawako Moriyama, Jing Che, Yoshinori Moriyama, Kozo Iwasaki, Jian Feng Ma

NATURE PLANTS 1 ( 12 ) 2015年11月

Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat 査読

Kiyotaka Nagaki, Keisuke Tanaka, Naoki Yamaji, Hisato Kobayashi, Minoru Murata

Frontiers in Plant Science 6 ( OCTOBER ) 2015年10月

A node-localized transporter OsZIP3 is responsible for the preferential distribution of Zn to developing tissues in rice 査読

Akimasa Sasaki, Naoki Yamaji, Namiki Mitani-Ueno, Miho Kashino, Jian Feng Ma

PLANT JOURNAL 84 ( 2 ) 374 - 384 2015年10月

Orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice 査読

Naoki Yamaji, Gen Sakurai, Namiki Mitani-Ueno, Jian Feng Ma

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112 ( 36 ) 11401 - 11406 2015年9月

A cooperative system of silicon transport in plants 査読

Jian Feng Ma, Naoki Yamaji

TRENDS IN PLANT SCIENCE 20 ( 7 ) 435 - 442 2015年7月

In Silico Simulation Modeling Reveals the Importance of the Casparian Strip for Efficient Silicon Uptake in Rice Roots 査読

Gen Sakurai, Akiko Satake, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Francois Gabriel Feugier, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 56 ( 4 ) 631 - 639 2015年4月

The Rice CK2 Kinase Regulates Trafficking of Phosphate Transporters in Response to Phosphate Levels 査読

Jieyu Chen, Yifeng Wang, Fei Wang, Jian Yang, Mingxing Gao, Changying Li, Yingyao Liu, Yu Liu, Naoki Yamaji, Jian Feng Ma, Javier Paz-Ares, Laurent Nussaume, Shuqun Zhang, Keke Yi, Zhongchang Wu, Ping Wu

PLANT CELL 27 ( 3 ) 711 - 723 2015年3月

AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis 査読

Takaaki Miyaji, Takashi Kuromori, Yu Takeuchi, Naoki Yamaji, Kengo Yokosho, Atsushi Shimazawa, Eriko Sugimoto, Hiroshi Omote, Jian Feng Ma, Kazuo Shinozaki, Yoshinori Moriyama

NATURE COMMUNICATIONS 6 5928 2015年1月

植物栄養と数理モデルの接点ー数理モデルで植物栄養の仕組みを理解する

馬建鋒, 小林高範, 山地直樹, 小林安文, 小山博之, 岩本明敏, 櫻井玄

日本土壌肥料学会誌 86 ( 3 ) 226 - 230 2015年

記述言語：日本語出版者・発行元：日本土壌肥料學會

植物は生育に欠かせない14種類のミネラルを根により土壌から吸収し，根から地上部に輸送したあと，過不足無く各器官に分配する。近年これらのミネラルの吸収，転流，分配などに関与するトランスポーターが多く同定された。しかし，これらのトランスポーターは協調して働くと考えられるが，その統合的な理解はまだ不十分である。一方，植物は栄養不足やミネラル過剰などのミネラルストレスを常に受けているが，植物はこれらのストレスに対しても巧みな耐性機構を発達させてきた。近年ミネラルストレス耐性に関わる遺伝子が多く同定された。しかし，植物の細胞がどのようにこれらのミネラルストレスを感知し，またどのようにシグナルを下流に伝達するのかについてまだ研究が始まったばかりである。本資料では，若手研究者による最新の植物栄養学の成果を紹介するとともに，ミネラルの輸送を統合的に理解するために試みられているモデリングの研究を紹介し，植物栄養学の今後の発展の方向性を探りたい。

DOI： 10.20710/dojo.86.3_232

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その他リンク： http://agriknowledge.affrc.go.jp/RN/2010892619

Global Transcriptome Analysis of Al-Induced Genes in an Al-Accumulating Species, Common Buckwheat (Fagopyrum esculentum Moench) 査読

Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 55 ( 12 ) 2077 - 2091 2014年12月

A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain 査読

Won-Yong Song, Tomohiro Yamaki, Naoki Yamaji, Donghwi Ko, Ki-Hong Jung, Miho Fujii-Kashino, Gynheung An, Enrico Martinoia, Youngsook Lee, Jian Feng Ma

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111 ( 44 ) 15699 - 15704 2014年11月

Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn transporter genes in rice 査読

Akimasa Sasaki, Naoki Yamaji, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 65 ( 20 ) 6013 - 6021 2014年11月

The node, a hub for mineral nutrient distribution in graminaceous plants 査読

Naoki Yamaji, Jian Feng Ma

TRENDS IN PLANT SCIENCE 19 ( 9 ) 556 - 563 2014年9月

Timing of the G1/S transition in tobacco pollen vegetative cells as a primary step towards androgenesis in vitro 査読

Masaharu Kyo, Ai Nagano, Naoki Yamaji, Yuhki Hashimoto

PLANT CELL REPORTS 33 ( 9 ) 1595 - 1606 2014年9月

Differential expression of Nrat1 is responsible for Al-tolerance QTL on chromosome 2 in rice 査読

Jixing Xia, Naoki Yamaji, Jing Che, Ren Fang Shen, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 65 ( 15 ) 4297 - 4304 2014年8月

Physiological and molecular characterization of Si uptake in wild rice species 査読

Namiki Mitani-Ueno, Hisao Ogai, Naoki Yamaji, Jian Feng Ma

PHYSIOLOGIA PLANTARUM 151 ( 3 ) 200 - 207 2014年7月

Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation 査読

Matthew J. Milner, Namiki Mitani-Ueno, Naoki Yamaji, Kengo Yokosho, Eric Craft, Zhangjun Fei, Stephen Ebbs, M. Clemencia Zambrano, Jian Feng Ma, Leon V. Kochian

PLANT JOURNAL 78 ( 3 ) 398 - 410 2014年5月

Normal root elongation requires arginine produced by argininosuccinate lyase in rice 査読

Jixing Xia, Naoki Yamaji, Jing Che, Ren Fang Shen, Jian Feng Ma

PLANT JOURNAL 78 ( 2 ) 215 - 226 2014年4月

An appropriate concentration of arginine is required for normal root growth in rice 査読

Jixing Xia, Naoki Yamaji, Jian Feng Ma

Plant Signaling and Behavior 9 ( 4 ) e28717 2014年

An appropriate concentration of arginine is required for normal root growth in rice. 査読

Xia J, Yamaji N, Ma JF

Plant signaling & behavior 9 ( 3 ) e28717 2014年

A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice 査読

Fenglin Deng, Naoki Yamaji, Jixing Xia, Jian Feng Ma

PLANT PHYSIOLOGY 163 ( 3 ) 1353 - 1362 2013年11月

Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family 査読

Zonghui Chen, Yumi Fujii, Naoki Yamaji, Sakine Masuda, Yuma Takemoto, Takehiro Kamiya, Yusufujiang Yusuyin, Kozo Iwasaki, Shin Ichiro Kato, Masayoshi Maeshima, Jian Feng Ma, Daisei Ueno

Journal of Experimental Botany 64 ( 14 ) 4375 - 4387 2013年11月

A plasma membrane-localized small peptide is involved in rice aluminum tolerance 査読

Jixing Xia, Naoki Yamaji, Jian Feng Ma

PLANT JOURNAL 76 ( 2 ) 345 - 355 2013年10月

Adaptation to acidic soil is achieved by increased numbers of cis-acting elements regulating ALMT1 expression in Holcus lanatus 査読

Zhi Chang Chen, Kengo Yokosho, Miho Kashino, Fang-Jie Zhao, Naoki Yamaji, Jian Feng Ma

PLANT JOURNAL 76 ( 1 ) 10 - 23 2013年10月

A node-based switch for preferential distribution of manganese in rice 査読

Naoki Yamaji, Akimasa Sasaki, Ji Xing Xia, Kengo Yokosho, Jian Feng Ma

NATURE COMMUNICATIONS 4 2442 2013年9月

Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2 査読

Naoki Yamaji, Jixing Xia, Namiki Mitani-Ueno, Kengo Yokosho, Jian Feng Ma

PLANT PHYSIOLOGY 162 ( 2 ) 927 - 939 2013年6月

Divergence of expression pattern contributed to neofunctionalization of duplicated HD-Zip I transcription factor in barley 査読

Shun Sakuma, Mohammad Pourkheirandish, Goetz Hensel, Jochen Kumlehn, Nils Stein, Akemi Tagiri, Naoki Yamaji, Jian Feng Ma, Hidenori Sassa, Takato Koba, Takao Komatsuda

NEW PHYTOLOGIST 197 ( 3 ) 939 - 948 2013年2月

Divergence of expression pattern contributed to neofunctionalization of duplicated HD-Zip I transcription factor in barley 査読

Shun Sakuma, Mohammad Pourkheirandish, Goetz Hensel, Jochen Kumlehn, Nils Stein, Akemi Tagiri, Naoki Yamaji, Jian Feng Ma, Hidenori Sassa, Takato Koba, Takao Komatsuda

NEW PHYTOLOGIST 197 ( 3 ) 939 - 948 2013年2月

Chromosome Dynamics Visualized with an Anti-Centromeric Histone H3 Antibody in Allium 査読

Kiyotaka Nagaki, Maki Yamamoto, Naoki Yamaji, Yasuhiko Mukai, Minoru Murata

PLOS ONE 7 ( 12 ) 2012年12月

Chromosome Dynamics Visualized with an Anti-Centromeric Histone H3 Antibody in Allium 査読

Kiyotaka Nagaki, Maki Yamamoto, Naoki Yamaji, Yasuhiko Mukai, Minoru Murata

PLOS ONE 7 ( 12 ) 2012年12月

Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley 査読

Naoki Yamaji, Yukako Chiba, Namiki Mitani-Ueno, Jian Feng Ma

PLANT PHYSIOLOGY 160 ( 3 ) 1491 - 1497 2012年11月

Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley 査読

Naoki Yamaji, Yukako Chiba, Namiki Mitani-Ueno, Jian Feng Ma

PLANT PHYSIOLOGY 160 ( 3 ) 1491 - 1497 2012年11月

Comparative Genome-Wide Transcriptional Analysis of Al-Responsive Genes Reveals Novel Al Tolerance Mechanisms in Rice 査読

Tomokazu Tsutsui, Naoki Yamaji, Chao Feng Huang, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

PLOS ONE 7 ( 10 ) 2012年10月

Comparative Genome-Wide Transcriptional Analysis of Al-Responsive Genes Reveals Novel Al Tolerance Mechanisms in Rice 査読

Tomokazu Tsutsui, Naoki Yamaji, Chao Feng Huang, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

PLOS ONE 7 ( 10 ) 2012年10月

Comparative Genome-Wide Transcriptional Analysis of Al-Responsive Genes Reveals Novel Al Tolerance Mechanisms in Rice 査読

Tomokazu Tsutsui, Naoki Yamaji, Chao Feng Huang, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

PLOS ONE 7 ( 10 ) 2012年10月

YSL16 Is a Phloem-Localized Transporter of the Copper-Nicotianamine Complex That Is Responsible for Copper Distribution in Rice 査読

Luqing Zheng, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

PLANT CELL 24 ( 9 ) 3767 - 3782 2012年9月

YSL16 Is a Phloem-Localized Transporter of the Copper-Nicotianamine Complex That Is Responsible for Copper Distribution in Rice 査読

Luqing Zheng, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

PLANT CELL 24 ( 9 ) 3767 - 3782 2012年9月

Up-Regulation of a Magnesium Transporter Gene OsMGT1 Is Required for Conferring Aluminum Tolerance in Rice 査読

Zhi Chang Chen, Naoki Yamaji, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

PLANT PHYSIOLOGY 159 ( 4 ) 1624 - 1633 2012年8月

Up-Regulation of a Magnesium Transporter Gene OsMGT1 Is Required for Conferring Aluminum Tolerance in Rice 査読

Zhi Chang Chen, Naoki Yamaji, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

PLANT PHYSIOLOGY 159 ( 4 ) 1624 - 1633 2012年8月

Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation 査読

Matthew J. Milner, Eric Craft, Naoki Yamaji, Emi Koyama, Jian Feng Ma, Leon V. Kochian

NEW PHYTOLOGIST 195 ( 1 ) 113 - 123 2012年7月

Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation 査読

Matthew J. Milner, Eric Craft, Naoki Yamaji, Emi Koyama, Jian Feng Ma, Leon V. Kochian

NEW PHYTOLOGIST 195 ( 1 ) 113 - 123 2012年7月

Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice 査読

Akimasa Sasaki, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

PLANT CELL 24 ( 5 ) 2155 - 2167 2012年5月

Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene 査読

Jonatan Montpetit, Julien Vivancos, Namiki Mitani-Ueno, Naoki Yamaji, Wilfried Remus-Borel, Francois Belzile, Jian Feng Ma, Richard R. Belanger

PLANT MOLECULAR BIOLOGY 79 ( 1-2 ) 35 - 46 2012年5月

Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice 査読

Akimasa Sasaki, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

PLANT CELL 24 ( 5 ) 2155 - 2167 2012年5月

Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene 査読

Jonatan Montpetit, Julien Vivancos, Namiki Mitani-Ueno, Naoki Yamaji, Wilfried Remus-Borel, Francois Belzile, Jian Feng Ma, Richard R. Belanger

PLANT MOLECULAR BIOLOGY 79 ( 1-2 ) 35 - 46 2012年5月

Acquisition of aluminium tolerance by modification of a single gene in barley. 査読国際誌

Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takahashi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

Nature communications 3 713 - 713 2012年3月

Acquisition of aluminium tolerance by modification of a single gene in barley. 査読国際誌

Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takahashi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

Nature communications 3 713 - 713 2012年3月

記述言語：英語

Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.

DOI： 10.1038/ncomms1726

PubMed

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A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice 査読

Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

PLANT JOURNAL 69 ( 5 ) 857 - 867 2012年3月

A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice 査読

Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

PLANT JOURNAL 69 ( 5 ) 857 - 867 2012年3月

A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice 査読

Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

Plant Journal 69 ( 5 ) 857 - 867 2012年3月

A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots 査読

Chao-Feng Huang, Naoki Yamaji, Kazuko Ono, Jian Feng Ma

PLANT JOURNAL 69 ( 4 ) 565 - 576 2012年2月

A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots 査読

Chao-Feng Huang, Naoki Yamaji, Kazuko Ono, Jian Feng Ma

PLANT JOURNAL 69 ( 4 ) 565 - 576 2012年2月

OsYSL6 Is Involved in the Detoxification of Excess Manganese in Rice 査読

Akimasa Sasaki, Naoki Yamaji, Jixing Xia, Jian Feng Ma

PLANT PHYSIOLOGY 157 ( 4 ) 1832 - 1840 2011年12月

An Al-inducible MATE gene is involved in external detoxification of Al in rice 査読

Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

PLANT JOURNAL 68 ( 6 ) 1061 - 1069 2011年12月

OsYSL6 Is Involved in the Detoxification of Excess Manganese in Rice 査読

Akimasa Sasaki, Naoki Yamaji, Jixing Xia, Jian Feng Ma

PLANT PHYSIOLOGY 157 ( 4 ) 1832 - 1840 2011年12月

An Al-inducible MATE gene is involved in external detoxification of Al in rice 査読

Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

PLANT JOURNAL 68 ( 6 ) 1061 - 1069 2011年12月

A functional cutin matrix is required for plant protection against water loss 査読

Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Chao Li, Naoki Yamaji, Eviatar Nevo

Plant Signaling and Behavior 6 ( 9 ) 1297 - 1299 2011年9月

The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic 査読

Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 62 ( 12 ) 4391 - 4398 2011年8月

The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic 査読

Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 62 ( 12 ) 4391 - 4398 2011年8月

Transport of silicon from roots to panicles in plants 査読

Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES 87 ( 7 ) 377 - 385 2011年7月

Transport of silicon from roots to panicles in plants 査読

Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES 87 ( 7 ) 377 - 385 2011年7月

An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice 査読

Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Christiane Nawrath, Mohammad Pourkheirandish, Akemi Tagiri, Yin-Gang Hu, Mohammad Sameri, Xinrong Li, Xin Zhao, Yubing Liu, Chao Li, Xiaoying Ma, Aidong Wang, Sudha Nair, Ning Wang, Akio Miyao, Shun Sakuma, Naoki Yamaji, Xiuting Zheng, Eviatar Nevo

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108 ( 30 ) 12354 - 12359 2011年7月

An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice 査読

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108 ( 30 ) 12354 - 12359 2011年7月

Transport of silicon from roots to panicles in plants 査読

Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES 87 ( 7 ) 377 - 385 2011年7月

Identification of a Cis-Acting Element of ART1, a C2H2-Type Zinc-Finger Transcription Factor for Aluminum Tolerance in Rice 査読

Tomokazu Tsutsui, Naoki Yamaji, Jian Feng Ma

PLANT PHYSIOLOGY 156 ( 2 ) 925 - 931 2011年6月

Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd-hyperaccumulating ecotype of Thlaspi caerulescens 査読

Daisei Ueno, Matthew J. Milner, Naoki Yamaji, Kengo Yokosho, Emi Koyama, M. Clemencia Zambrano, Molly Kaskie, Stephen Ebbs, Leon V. Kochian, Jian Feng Ma

PLANT JOURNAL 66 ( 5 ) 852 - 862 2011年6月

Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd-hyperaccumulating ecotype of Thlaspi caerulescens 査読

Daisei Ueno, Matthew J. Milner, Naoki Yamaji, Kengo Yokosho, Emi Koyama, M. Clemencia Zambrano, Molly Kaskie, Stephen Ebbs, Leon V. Kochian, Jian Feng Ma

PLANT JOURNAL 66 ( 5 ) 852 - 862 2011年6月

Identification of a Cis-Acting Element of ART1, a C2H2-Type Zinc-Finger Transcription Factor for Aluminum Tolerance in Rice 査読

Tomokazu Tsutsui, Naoki Yamaji, Jian Feng Ma

PLANT PHYSIOLOGY 156 ( 2 ) 925 - 931 2011年6月

Isolation and characterization of a barley yellow stripe-like gene, HvYSL5. 査読

Luqing Zheng, Miho Fujii, Naoki Yamaji, Akimasa Sasaki, Miki Yamane, Isamu Sakurai, Kazuhiro Sato, Jian Feng Ma

Plant & cell physiology 52 ( 5 ) 765 - 74 2011年5月

Isolation and characterization of a barley yellow stripe-like gene, HvYSL5. 査読

Luqing Zheng, Miho Fujii, Naoki Yamaji, Akimasa Sasaki, Miki Yamane, Isamu Sakurai, Kazuhiro Sato, Jian Feng Ma

Plant & cell physiology 52 ( 5 ) 765 - 74 2011年5月

Further characterization of a rice silicon efflux transporter, Lsi2 査読

Naoki Yamaji, Jian Feng Ma

SOIL SCIENCE AND PLANT NUTRITION 57 ( 2 ) 259 - 264 2011年4月

Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation 査読

Namiki Mitani, Naoki Yamaji, Yukiko Ago, Kozo Iwasaki, Jian Feng Ma

PLANT JOURNAL 66 ( 2 ) 231 - 240 2011年4月

Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation 査読

Namiki Mitani, Naoki Yamaji, Yukiko Ago, Kozo Iwasaki, Jian Feng Ma

PLANT JOURNAL 66 ( 2 ) 231 - 240 2011年4月

Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan 査読

Daisei Ueno, Emi Koyama, Naoki Yamaji, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 62 ( 7 ) 2265 - 2272 2011年4月

Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan 査読

Daisei Ueno, Emi Koyama, Naoki Yamaji, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 62 ( 7 ) 2265 - 2272 2011年4月

Further characterization of a rice silicon efflux transporter, Lsi2 査読

Naoki Yamaji, Jian Feng Ma

SOIL SCIENCE AND PLANT NUTRITION 57 ( 2 ) 259 - 264 2011年4月

Further characterization of an aluminum influx transporter in rice 査読

Jixing Xia, Naoki Yamaji, Jian Feng Ma

Plant Signaling and Behavior 6 ( 1 ) 160 - 163 2011年1月

Further characterization of a rice silicon efflux transporter, Lsi2 査読

Naoki Yamaji, Jian Feng Ma

Soil Science and Plant Nutrition 57 ( 2 ) 259 - 264 2011年

Plasma membrane-localized transporter for aluminum in rice 査読

Jixing Xia, Naoki Yamaji, Tomonari Kasai, Jian Feng Ma

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107 ( 43 ) 18381 - 18385 2010年10月

Plasma membrane-localized transporter for aluminum in rice 査読

Jixing Xia, Naoki Yamaji, Tomonari Kasai, Jian Feng Ma

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107 ( 43 ) 18381 - 18385 2010年10月

Gene limiting cadmium accumulation in rice 査読

Daisei Ueno, Naoki Yamaji, Izumi Kono, Chao Feng Huang, Tsuyu Ando, Masahiro Yano, Jian Feng Ma

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107 ( 38 ) 16500 - 16505 2010年9月

Gene limiting cadmium accumulation in rice 査読

Daisei Ueno, Naoki Yamaji, Izumi Kono, Chao Feng Huang, Tsuyu Ando, Masahiro Yano, Jian Feng Ma

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107 ( 38 ) 16500 - 16505 2010年9月

Knockout of a Bacterial-Type ATP-Binding Cassette Transporter Gene, AtSTAR1, Results in Increased Aluminum Sensitivity in Arabidopsis 査読

Chao-Feng Huang, Naoki Yamaji, Jian Feng Ma

PLANT PHYSIOLOGY 153 ( 4 ) 1669 - 1677 2010年8月

Involvement of Silicon Influx Transporter OsNIP2;1 in Selenite Uptake in Rice 査読

Xue Qiang Zhao, Namiki Mitani, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

PLANT PHYSIOLOGY 153 ( 4 ) 1871 - 1877 2010年8月

Involvement of Silicon Influx Transporter OsNIP2;1 in Selenite Uptake in Rice 査読

Xue Qiang Zhao, Namiki Mitani, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

PLANT PHYSIOLOGY 153 ( 4 ) 1871 - 1877 2010年8月

Knockout of a Bacterial-Type ATP-Binding Cassette Transporter Gene, AtSTAR1, Results in Increased Aluminum Sensitivity in Arabidopsis 査読

Chao-Feng Huang, Naoki Yamaji, Jian Feng Ma

PLANT PHYSIOLOGY 153 ( 4 ) 1669 - 1677 2010年8月

A specific transporter for iron(III)-phytosiderophore in barley roots (vol 46, pg 563, 2006) 査読

Y. Murata, J. F. Ma, N. Yamaji, D. Ueno, K. Nomoto, T. Iwashita

PLANT JOURNAL 61 ( 1 ) 188 - 188 2010年1月

A specific transporter for iron(III)-phytosiderophore in barley roots (vol 46, pg 563, 2006) 査読

Y. Murata, J. F. Ma, N. Yamaji, D. Ueno, K. Nomoto, T. Iwashita

PLANT JOURNAL 61 ( 1 ) 188 - 188 2010年1月

The role of the rice aquaporin Lsi1 in arsenite efflux from roots 査読

Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

NEW PHYTOLOGIST 186 ( 2 ) 392 - 399 2010年

Isolation and characterisation of two MATE genes in rye 査読

Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

FUNCTIONAL PLANT BIOLOGY 37 ( 4 ) 296 - 303 2010年

Isolation and characterisation of two MATE genes in rye 査読

Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

FUNCTIONAL PLANT BIOLOGY 37 ( 4 ) 296 - 303 2010年

The role of the rice aquaporin Lsi1 in arsenite efflux from roots 査読

Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

NEW PHYTOLOGIST 186 ( 2 ) 392 - 399 2010年

Isolation and characterisation of two MATE genes in rye 査読

Naoki Yamaji

Functional Plant Biology 37 ( 4 ) 2010年

The role of the rice aquaporin Lsi1 in arsenite efflux from roots 査読

Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

NEW PHYTOLOGIST 186 ( 2 ) 392 - 399 2010年

A Zinc Finger Transcription Factor ART1 Regulates Multiple Genes Implicated in Aluminum Tolerance in Rice 査読

Naoki Yamaji, Chao Feng Huang, Sakiko Nagao, Masahiro Yano, Yutaka Sato, Yoshiaki Nagamura, Jian Feng Ma

PLANT CELL 21 ( 10 ) 3339 - 3349 2009年10月

A Transporter at the Node Responsible for Intervascular Transfer of Silicon in Rice 査読

Naoki Yamaji, Jian Feng Ma

PLANT CELL 21 ( 9 ) 2878 - 2883 2009年9月

Further characterization of ferric-phytosiderophore transporters ZmYS1 and HvYS1 in maize and barley 査読

Daisei Ueno, Naoki Yamaji, Jian Feng Ma

JOURNAL OF EXPERIMENTAL BOTANY 60 ( 12 ) 3513 - 3520 2009年8月

Identification and Characterization of Maize and Barley Lsi2-Like Silicon Efflux Transporters Reveals a Distinct Silicon Uptake System from That in Rice 査読

Namiki Mitani, Yukako Chiba, Naoki Yamaji, Jian Feng Ma

PLANT CELL 21 ( 7 ) 2133 - 2142 2009年7月

A Rice Mutant Sensitive to Al Toxicity is Defective in the Specification of Root Outer Cell Layers 査読

Chao-Feng Huang, Naoki Yamaji, Minoru Nishimura, Shigeyuki Tajima, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 50 ( 5 ) 976 - 985 2009年5月

HvLsi1 is a silicon influx transporter in barley 査読

Yukako Chiba, Namiki Mitani, Naoki Yamaji, Jian Feng Ma

PLANT JOURNAL 57 ( 5 ) 810 - 818 2009年3月

HvLsi1 is a silicon influx transporter in barley 査読

Yukako Chiba, Namiki Mitani, Naoki Yamaji, Jian Feng Ma

PLANT JOURNAL 57 ( 5 ) 810 - 818 2009年3月

A Bacterial-Type ABC Transporter Is Involved in Aluminum Tolerance in Rice 査読

Chao Feng Huang, Naoki Yamaji, Namiki Mitani, Masahiro Yano, Yoshiaki Nagamura, Jian Feng Ma

PLANT CELL 21 ( 2 ) 655 - 667 2009年2月

Identification of Maize Silicon Influx Transporters 査読

Namiki Mitani, Naoki Yamaji, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 50 ( 1 ) 5 - 12 2009年1月

OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice 査読

Kengo Yokosho, Naoki Yamaji, Daisei Ueno, Namiki Mitani, Jian Feng Ma

PLANT PHYSIOLOGY 149 ( 1 ) 297 - 305 2009年1月

Specific transporter for iron(III)-phytosiderophore complex involved in iron uptake by barley roots 査読

Yoshiko Murata, Emiko Harada, Kenji Sugase, Kosuke Namba, Manabu Horikawa, Jian Feng Ma, Naoki Yamaji, Daisei Ueno, Kyosuke Nomoto, Takashi Iwashita, Shoichi Kusumoto

PURE AND APPLIED CHEMISTRY 80 ( 12 ) 2689 - 2697 2008年12月

Functions and transport of silicon in plants 査読

J. F. Ma, N. Yamaji

CELLULAR AND MOLECULAR LIFE SCIENCES 65 ( 19 ) 3049 - 3057 2008年10月

Characterization of substrate specificity of a rice silicon transporter, Lsi1 査読

Namiki Mitani, Naoki Yamaji, Jian Feng Ma

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 456 ( 4 ) 679 - 686 2008年7月

Transporters of arsenite in rice and their role in arsenic accumulation in rice grain 査読

Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Xiao-Yan Xu, Yu-Hong Su, Steve P. McGrath, Fang-Jie Zhao

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 ( 29 ) 9931 - 9935 2008年7月

Characterization of substrate specificity of a rice silicon transporter, Lsi1 査読

Namiki Mitani, Naoki Yamaji, Jian Feng Ma

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 456 ( 4 ) 679 - 686 2008年7月

A transporter regulating silicon distribution in rice shoots 査読

Naoki Yamaji, Namiki Mitatni, Jian Feng Ma

PLANT CELL 20 ( 5 ) 1381 - 1389 2008年5月

Genotypic difference in silicon uptake and expression of silicon transporter genes in rice 査読

Jian Feng Ma, Naoki Yamaji, Kazunori Tamai, Namiki Mitani

PLANT PHYSIOLOGY 145 ( 3 ) 919 - 924 2007年11月

Silicon transporters in rice

Jian Feng Ma, Naoki Yamaji, Namiki Mitani

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 52 ( 14 ) 1849 - 1856 2007年11月

Shoot formation from root tip region: a developmental alteration by WUS in transgenic tobacco 査読

Syeda Zinia Rashid, Naoki Yamaji, Masaharu Kyo

PLANT CELL REPORTS 26 ( 9 ) 1449 - 1455 2007年9月

An aluminum-activated citrate transporter in barley. 査読

Jun Furukawa, Naoki Yamaji, Hua Wang, Namiki Mitani, Yoshiko Murata, Kazuhiro Sato, Maki Katsuhara, Kazuyoshi Takeda, Jian Feng Ma

Plant & cell physiology 48 ( 8 ) 1081 - 91 2007年8月

記述言語：英語

Soluble ionic aluminum (Al) inhibits root growth and reduces crop production on acid soils. Al-resistant cultivars of barley (Hordeum vulgare L.) detoxify Al by secreting citrate from the roots, but the responsible gene has not been identified yet. Here, we identified a gene (HvAACT1) responsible for the Al-activated citrate secretion by fine mapping combined with microarray analysis, using an Al-resistant cultivar, Murasakimochi, and an Al-sensitive cultivar, Morex. This gene belongs to the multidrug and toxic compound extrusion (MATE) family and was constitutively expressed mainly in the roots of the Al-resistant barley cultivar. Heterologous expression of HvAACT1 in Xenopus oocytes showed efflux activity for (14)C-labeled citrate, but not for malate. Two-electrode voltage clamp analysis also showed transport activity of citrate in the HvAACT1-expressing oocytes in the presence of Al. Overexpression of this gene in tobacco enhanced citrate secretion and Al resistance compared with the wild-type plants. Transiently expressed green fluorescent protein-tagged HvAACT1 was localized at the plasma membrane of the onion epidermal cells, and immunostaining showed that HvAACT1 was localized in the epidermal cells of the barley root tips. A good correlation was found between the expression of HvAACT1 and citrate secretion in 10 barley cultivars differing in Al resistance. Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley.

DOI： 10.1093/pcp/pcm091

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An efflux transporter of silicon in rice 査読

Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

NATURE 448 ( 7150 ) 209 - U12 2007年7月

Sorghum roots are inefficient in uptake of EDTA-chelated lead 査読

Yong Xu, Naoki Yamaji, Renfang Shen, Jian Feng Ma

ANNALS OF BOTANY 99 ( 5 ) 869 - 875 2007年5月

Spatial distribution and temporal variation of the rice silicon transporter Lsi1 査読

Naoki Yamaji, Jian Feng Ma

PLANT PHYSIOLOGY 143 ( 3 ) 1306 - 1313 2007年3月

Characterization of silicon permeability of NIP in gramineous plants 査読

Namiki Mitani, Naoki Yamaji, Maki Katsuhara, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 48 S30 - S30 2007年

Functional analysis of a rice Al-tolerant gene Als1 査読

Chaofeng Huang, Naoki Yamaji, Masahiro Yano, Jianfeng Ma

PLANT AND CELL PHYSIOLOGY 48 S45 - S45 2007年

Further characterization of an Fe-phytosiderophore transporter gene ZmYS 1 in maize 査読

Daisei Ueno, Naoki Yamaji, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 48 S29 - S29 2007年

Functional analysis of a rice Si transporter homolog Lsi6. 査読

Naoki Yamaji, Namiki Mitani, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 48 S30 - S30 2007年

Gene expression and functional analysis of a candidate gene related to Al-activated citrate transporter in barley 査読

Jun Furukawa, Naoki Yamaji, Hua Wang, Maki Katsuhara, Kazuhiro Sato, Kazuyoshi Takeda, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 48 S44 - S44 2007年

Gene expression and functional analysis of a candidate gene related to Al-activated citrate transporter in barley 査読

Jun Furukawa, Naoki Yamaji, Hua Wang, Maki Katsuhara, Kazuhiro Sato, Kazuyoshi Takeda, Jian Feng Ma

PLANT AND CELL PHYSIOLOGY 48 S44 - S44 2007年

Silicon uptake and accumulation in higher plants 査読

Jian Feng Ma, Naoki Yamaji

TRENDS IN PLANT SCIENCE 11 ( 8 ) 392 - 397 2006年8月

Silicon uptake and accumulation in higher plants 査読

Jian Feng Ma, Naoki Yamaji

TRENDS IN PLANT SCIENCE 11 ( 8 ) 392 - 397 2006年8月

Two promoters conferring active gene expression in vegetative nuclei of tobacco immature pollen undergoing embryogenic dedifferentiation 査読

Naoki Yamaji, Masaharu Kyo

PLANT CELL REPORTS 25 ( 8 ) 749 - 757 2006年8月

P-157 A SPECIFIC TRANSPORTER OF IRON (III)-PHYTOSIDEROPHORE, MUGINEIC ACID IN BARLEY ROOTS

Murata Yoshiko, Ma Jian Feng, Yamaji Naoki, Ueno Daisei, Nomoto Kyosuke, Iwashita Takashi

International Symposium on the Chemistry of Natural Products 2006 "P - 157" 2006年7月

A specific transporter for iron(III)-phytosiderophore in barley roots 査読

Y Murata, JF Ma, N Yamaji, D Ueno, K Nomoto, T Iwashita

PLANT JOURNAL 46 ( 4 ) 563 - 572 2006年5月

A specific transporter for iron(III)-phytosiderophore in barley roots 査読

Y Murata, JF Ma, N Yamaji, D Ueno, K Nomoto, T Iwashita

PLANT JOURNAL 46 ( 4 ) 563 - 572 2006年5月

A silicon transporter in rice 査読

JF Ma, K Tamai, N Yamaji, N Mitani, S Konishi, M Katsuhara, M Ishiguro, Y Murata, M Yano

NATURE 440 ( 7084 ) 688 - 691 2006年3月

Functional characterization of a Si transporter gene Lsi1 in rice 査読

N Mitani, N Yamaji, K Tamai, S Konishi, M Yano, JF Ma

PLANT AND CELL PHYSIOLOGY 47 S61 - S61 2006年

Functional analysis of rice gene Lsi2 related to Si uptake 査読

N Yamaji, JF Ma

PLANT AND CELL PHYSIOLOGY 47 S61 - S61 2006年

Isolation and characterization of Lsi1 査読

N Mitani, K Tamai, S Konishi, M Yano, N Yamaji, M Kyo, JF Ma

PLANT AND CELL PHYSIOLOGY 46 S82 - S82 2005年

Cloning and characterization of cDNAs associated with the embryogenic dedifferentiation of tobacco immature pollen grains 査読

M Kyo, S Hattori, N Yamaji, P Pechan, H Fukui

PLANT SCIENCE 164 ( 6 ) 1057 - 1066 2003年6月

Isolation of cDNAs coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system 査読

M Kyo, N Yamaji, Y Yuasa, T Maeda, H Fukui

PLANT SCIENCE 163 ( 5 ) 1055 - 1061 2002年11月

Isolation of promoter elements conferring the expression pattern associated with the embryogenic dedifferentiation in a tobacco pollen culture system 査読

N Yamaji, M Kyo

PLANT AND CELL PHYSIOLOGY 43 S132 - S132 2002年

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