研究者詳細 - 山地　直樹

論文 - 山地　直樹

分割表示 229 件中 201 - 220 件目／全件表示 >>

Transporters of arsenite in rice and their role in arsenic accumulation in rice grain 査読

Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Xiao-Yan Xu, Yu-Hong Su, Steve P. McGrath, Fang-Jie Zhao

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 ( 29 ) 9931 - 9935 2008年7月

Characterization of substrate specificity of a rice silicon transporter, Lsi1 査読

Namiki Mitani, Naoki Yamaji, Jian Feng Ma

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 456 ( 4 ) 679 - 686 2008年7月

A transporter regulating silicon distribution in rice shoots 査読

Naoki Yamaji, Namiki Mitatni, Jian Feng Ma

PLANT CELL 20 ( 5 ) 1381 - 1389 2008年5月

Genotypic difference in silicon uptake and expression of silicon transporter genes in rice 査読

Jian Feng Ma, Naoki Yamaji, Kazunori Tamai, Namiki Mitani

PLANT PHYSIOLOGY 145 ( 3 ) 919 - 924 2007年11月

Silicon transporters in rice

Jian Feng Ma, Naoki Yamaji, Namiki Mitani

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 52 ( 14 ) 1849 - 1856 2007年11月

Shoot formation from root tip region: a developmental alteration by WUS in transgenic tobacco 査読

Syeda Zinia Rashid, Naoki Yamaji, Masaharu Kyo

PLANT CELL REPORTS 26 ( 9 ) 1449 - 1455 2007年9月

An aluminum-activated citrate transporter in barley. 査読

Jun Furukawa, Naoki Yamaji, Hua Wang, Namiki Mitani, Yoshiko Murata, Kazuhiro Sato, Maki Katsuhara, Kazuyoshi Takeda, Jian Feng Ma

Plant & cell physiology 48 ( 8 ) 1081 - 91 2007年8月

記述言語：英語

Soluble ionic aluminum (Al) inhibits root growth and reduces crop production on acid soils. Al-resistant cultivars of barley (Hordeum vulgare L.) detoxify Al by secreting citrate from the roots, but the responsible gene has not been identified yet. Here, we identified a gene (HvAACT1) responsible for the Al-activated citrate secretion by fine mapping combined with microarray analysis, using an Al-resistant cultivar, Murasakimochi, and an Al-sensitive cultivar, Morex. This gene belongs to the multidrug and toxic compound extrusion (MATE) family and was constitutively expressed mainly in the roots of the Al-resistant barley cultivar. Heterologous expression of HvAACT1 in Xenopus oocytes showed efflux activity for (14)C-labeled citrate, but not for malate. Two-electrode voltage clamp analysis also showed transport activity of citrate in the HvAACT1-expressing oocytes in the presence of Al. Overexpression of this gene in tobacco enhanced citrate secretion and Al resistance compared with the wild-type plants. Transiently expressed green fluorescent protein-tagged HvAACT1 was localized at the plasma membrane of the onion epidermal cells, and immunostaining showed that HvAACT1 was localized in the epidermal cells of the barley root tips. A good correlation was found between the expression of HvAACT1 and citrate secretion in 10 barley cultivars differing in Al resistance. Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley.

DOI： 10.1093/pcp/pcm091

PubMed

researchmap

An efflux transporter of silicon in rice 査読

Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

NATURE 448 ( 7150 ) 209 - U12 2007年7月