Faculty Profiles - EKUNI Daisuke

Papers - EKUNI Daisuke

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Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria. International journal

Hirofumi Mizuno, Eiji Takayama, Ayano Satoh, Takeshi Into, Masanori Adachi, Daisuke Ekuni, Koji Yashiro, Masako Mizuno-Kamiya, Motohiko Nagayama, Seitaro Saku, Takaaki Tomofuji, Yutaka Doi, Yukitaka Murakami, Nobuo Kondoh, Manabu Morita

Experimental and therapeutic medicine 20 ( 3 ) 2822 - 2827 2020.9

Language：English Publishing type：Research paper (scientific journal)

Salivary peroxidase and myeloperoxidase are known to display antibacterial activity against oral microbes, and previous indications have pointed to the possibility that horseradish peroxidase (HRP) adsorbs onto the membrane of the major oral streptococci, Streptococcus mutans and Streptococcus sanguinis (S. sanguinis). However, the mechanism of interaction between HRP and the bacterial cell wall component is unclear. Dental plaques containing salivary glycoproteins and extracellular microbial products are visualized with 'dental plaque disclosing agent', and are controlled within dental therapy. However, current 'dental plaque disclosing agents' are difficult to evaluate with just dental plaques, since they stain and disclose not only dental plaques but also pellicle formed with salivary glycoproteins on a tooth surface. In this present study, we have demonstrated that HRP interacted with the cell wall component of the major gram-positive bacterial peptidoglycan, but not the major cell wall component of gram-negative bacteria lipopolysaccharide. Furthermore, we observed that the adsorbed HRP labeled with fluorescence was detected on the major oral gram-positive strains S. sanguinis and Streptococcus salivarius (S. salivarius), but not on a gram-negative strain, Escherichia coli (E. coli). Furthermore, we have demonstrated that the combination of HRP and chromogenic substrate clearly disclosed the dental plaques and the biofilm developed by S. sanguinis, S. salivarius and the major gram-postive bacteria Lactobacillus casei on tooth surfaces, and slightly disclosed the biofilm by E. coli. The combination of HRP and chromogenic substrate did not stain either the dental pellicle with the salivary glycoprotein mucin, or naked tooth surfaces. These results have suggested the possibility that the adsorption activity of HRP not only contributes to the evaluation of dental plaque, but that enzymatic activity of HRP may also contribute to improve dental hygiene.

DOI： 10.3892/etm.2020.9016

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抗菌的光線力学療法が歯肉縁下細菌叢の形成に与える影響について

入江浩一郎, 田畑綾乃, 内田瑶子, 江國大輔, 友藤孝明, 森田学

口腔衛生学会雑誌 70 ( 3 ) 144 - 151 2020.7

Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study. International journal

Yoko Uchida-Fukuhara, Daisuke Ekuni, Md Monirul Islam, Kota Kataoka, Ayano Taniguchi-Tabata, Daiki Fukuhara, Naoki Toyama, Terumasa Kobayashi, Kohei Fujimori, Nanami Sawada, Yoshiaki Iwasaki, Manabu Morita

International journal of environmental research and public health 17 ( 10 ) 2020.5

歯周病試験紙を応用した職域における歯科検診の試み

山本龍生, 渕田慎也, 持田悠貴, 江國大輔, 森田学, 恒石美登里

産業衛生学雑誌 62 ( 臨増 ) 406 - 406 2020.5

Association between Sleep Quality and Duration and Periodontal Disease among University Students: A Cross-Sectional Study. International journal

Md Monirul Islam, Daisuke Ekuni, Naoki Toyama, Ayano Taniguchi-Tabata, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Hikari Saho, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

International journal of environmental research and public health 17 ( 9 ) 2020.4

Language：English Publishing type：Research paper (scientific journal)

The purpose of this cross-sectional study was to investigate the association between sleep quality and duration, and periodontal disease among a group of young Japanese university students. First-year students (n = 1934) at Okayama University who voluntarily underwent oral health examinations were included in the analysis. Sleep quality and duration were assessed by the Japanese version of the Pittsburgh Sleep Quality Index. Dentists examined Oral Hygiene Index-Simplified (OHI-S), probing pocket depth (PPD), and percentage of sites with bleeding on probing (BOP). Periodontal disease was defined as presence of PPD ≥ 4 mm and BOP ≥ 30%. Overall, 283 (14.6%) students had periodontal disease. Poor sleep quality was observed among 372 (19.2%) students. Mean (± standard deviation) sleep duration was 7.1 ± 1.1 (hours/night). In the logistic regression analysis, periodontal disease was significantly associated with OHI-S (odds ratio [OR]: 2.30, 95% confident interval [CI]: 1.83-2.90; p < 0.001), but not sleep quality (OR: 1.09, 95% CI: 0.79-1.53; p = 0.577) or sleep duration (OR: 0.98, CI: 0.87-1.10; p = 0.717). In conclusion, sleep quality and duration were not associated with periodontal disease among this group of young Japanese university students.

DOI： 10.3390/ijerph17093034

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Associations between sleep bruxism, sleep quality, and exposure to secondhand smoke in Japanese young adults: a cross-sectional study. International journal

Naoki Toyama, Daisuke Ekuni, Ayano Taniguchi-Tabata, Toshiki Yoneda, Kota Kataoka, Aya Yokoi, Yoko Uchida, Daiki Fukuhara, Hikari Saho, Islam Md Monirul, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

Sleep medicine 68 57 - 62 2020.4

Relationship of Salivary Microbiome with the Worsening of the Periodontal Health Status in Young Adults: A 3-Year Cohort Study. International journal

Md Monirul Islam, Daisuke Ekuni, Naoki Toyama, Terumasa Kobayashi, Kohei Fujimori, Yoko Uchida, Daiki Fukuhara, Ayano Taniguchi-Tabata, Kota Kataoka, Yoshiaki Iwasaki, Manabu Morita

International journal of environmental research and public health 17 ( 5 ) 2020.3

慢性歯周炎患者の歯周状態の悪化に関連する唾液中miRNAの探索コホート研究

藤森浩平, 米田俊樹, 杉浦嘉雄, 丸山貴之, 江國大輔, 東哲司, 友藤孝明, 森田学

口腔衛生学会雑誌 70 ( 増刊 ) 158 - 158 2020.3

大学生における睡眠の質とストレスが歯周病に及ぼす影響(Influence of sleep quality and stress on periodontal disease among university students)

Islam Monirul, Ekuni Daisuke, Toyama Naoki, Tabata Ayano, Kataoka Kota, Uchida Yoko, Fukuhara Daiki, Saho Hikari, Sawada Nanami, Nakashima Yukiho, Morita Manabu

口腔衛生学会雑誌 70 ( 増刊 ) 159 - 159 2020.3

大学入学後の居住形態の変化と歯周病発症との関連(前向きコホート研究) Reviewed

中原桃子, 田畑綾乃, 外山直樹, 米田俊樹, 片岡広太, 佐保輝, Islam Monirul, 横井彩, 江國大輔, 森田学

口腔衛生学会雑誌 70 ( 増刊 ) 159 - 159 2020.3

Influence of sleep quality and stress on periodontal disease among university students(和訳中) Reviewed

Islam Monirul, Ekuni Daisuke, Toyama Naoki, Tabata Ayano, Kataoka Kota, Uchida Yoko, Fukuhara Daiki, Saho Hikari, Sawada Nanami, Nakashima Yukiho, Morita Manabu

口腔衛生学会雑誌 70 ( 増刊 ) 159 - 159 2020.3

大学入学後の居住形態の変化と歯周病発症との関連(前向きコホート研究)

中原桃子, 田畑綾乃, 外山直樹, 米田俊樹, 片岡広太, 佐保輝, Islam Monirul, 横井彩, 江國大輔, 森田学

口腔衛生学会雑誌 70 ( 増刊 ) 159 - 159 2020.3

Association between intensive health guidance focusing on eating quickly and metabolic syndrome in Japanese middle-aged citizens. International journal

Daisuke Ekuni, Michiko Furuta, Toshihide Kimura, Naoki Toyama, Daiki Fukuhara, Yoko Uchida, Nanami Sawada, Manabu Morita

Eating and weight disorders : EWD 25 ( 1 ) 91 - 98 2020.2

Language：English Publishing type：Research paper (scientific journal)

PURPOSE: The purpose of this intervention study was to investigate whether intensive health guidance focusing on eating quickly can prevent metabolic syndrome (MetS) more effectively than standard routine guidance in Japanese citizens living in rural areas. METHODS: This controlled, non-randomized, intervention study analyzed 141 participants with MetS at baseline. Participants in the intervention group received health guidance focusing on eating quickly and standard health guidance about MetS in accordance with the guidelines of the Ministry of Health, Labour and Welfare in Japan, whereas participants in the control group received only standard health guidance about MetS. The primary study outcome was the prevalence of MetS at a 1-year follow-up. RESULTS: At 1-year follow-up, the prevalence of MetS in the intervention group was significantly lower than that in the control group (p = 0.003). The decreases in body weight, body mass index, waist circumference and triglycerides from baseline to 1 year were significantly greater in the intervention group than in the control group (p < 0.05). CONCLUSION: Intensive health guidance focusing on eating quickly is more effective for improving MetS than standard Japanese health guidance alone. LEVEL OF EVIDENCE: Level II, Evidence obtained from well-designed controlled trials without randomization. TRIAL REGISTRY NAME, REGISTRATION IDENTIFICATION NUMBER, AND URL FOR THE REGISTRY: UMIN, UMIN000030600, http://www.umin.ac.jp/ctr/index-j.htm.

DOI： 10.1007/s40519-018-0522-1

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Association between maternal periodontal status and ultrasonographic measurement of fetal growth: A longitudinal study. International journal

Ayano Taniguchi-Tabata, Noriko Takeuchi, Yoko Uchida, Daisuke Ekuni, Manabu Morita

Scientific reports 10 ( 1 ) 1402 - 1402 2020.1

Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model

YOSHIO SUGIURA, TOSHIKI YONEDA, KOHEI FUJIMORI, TAKAYUKI MARUYAMA, HISATAKA MIYAI, TERUMASA KOBAYASHI, DAISUKE EKUNI, TAKAAKI TOMOFUJI, MANABU MORITA

In Vivo 34 ( 1 ) 117 - 123 2020

Effects of Coffee Intake on Oxidative Stress During Aging-related Alterations in Periodontal Tissue

TERUMASA KOBAYASHI, TAKAYUKI MARUYAMA, TOSHIKI YONEDA, HISATAKA MIYAI, TETSUJI AZUMA, TAKAAKI TOMOFUJI, DAISUKE EKUNI, MANABU MORITA

In Vivo 34 ( 2 ) 615 - 622 2020

Relationship between oral hygiene knowledge, source of oral hygiene knowledge and oral hygiene behavior in Japanese university students: A prospective cohort study. Reviewed International journal

Daiki Fukuhara, Daisuke Ekuni, Kota Kataoka, Ayano Taniguchi-Tabata, Yoko Uchida-Fukuhara, Naoki Toyama, Toshiki Yoneda, Yoshio Sugiura, Md Monirul Islam, Hikari Saho, Yoshiaki Iwasaki, Manabu Morita

PloS one 15 ( 7 ) e0236259 2020

Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model. International journal

Yoshio Sugiura, Toshiki Yoneda, Kohei Fujimori, Takayuki Maruyama, Hisataka Miyai, Terumasa Kobayashi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

In vivo (Athens, Greece) 34 ( 1 ) 117 - 123 2020

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND/AIM: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. MATERIALS AND METHODS: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. RESULTS: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a >2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. CONCLUSION: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model.

DOI： 10.21873/invivo.11752

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Effects of Coffee Intake on Oxidative Stress During Aging-related Alterations in Periodontal Tissue. International journal

Terumasa Kobayashi, Takayuki Maruyama, Toshiki Yoneda, Hisataka Miyai, Tetsuji Azuma, Takaaki Tomofuji, Daisuke Ekuni, Manabu Morita

In vivo (Athens, Greece) 34 ( 2 ) 615 - 622 2020

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND/AIM: The purpose of this study was to determine the anti-aging effects of coffee intake on oxidative stress in rat periodontal tissue and alveolar bone loss. MATERIALS AND METHODS: Male Fischer 344 rats (8 weeks old) were randomized to four groups; the baseline group immediately sacrificed, the control group fed with normal powdered food for 8 weeks, and the experimental groups fed with powdered food containing 0.62% or 1.36% coffee components for 8 weeks. RESULTS: Alveolar bone loss and gingival level of 8-hydroxydeoxyguanosine were significantly lower in the 1.36% coffee group than in the control group. Nuclear factor erythroid 2-related factor 2 translocation to the nucleus was significantly higher in the 1.36% coffee group than in the control group. CONCLUSION: Continuous intake of 1.36% coffee could prevent age-related oxidative stress in the periodontal tissue and alveolar bone loss, possibly by up-regulating the Nrf2 signaling pathway.

DOI： 10.21873/invivo.11815

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大学生における歯科に関する知識、歯科に関する知識の情報源及び口腔保健行動との関連:前向きコホート研究

福原大樹, 江國大輔, 片岡広太, 田畑綾乃, 内田瑶子, 外山直樹, 米田俊樹, 杉浦嘉雄, イスラム・モハマド・モニルル, 佐保輝, 森田学

岡山歯学会雑誌 38 ( 2 ) 93 - 93 2019.12

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