Faculty Profiles - FUNAHASHI Hiroaki

MISC - FUNAHASHI Hiroaki

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Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage

SH Oh, K Miyoshi, H Funahashi

BIOLOGY OF REPRODUCTION 59 ( 4 ) 884 - 889 1998.10

Language：English Publisher：SOC STUDY REPRODUCTION

A suitable chemically defined culture medium far 1-cell rat embryos (mR1ECM) was modified to obtain sperm penetration, and the developmental competence of oocytes fertilized in the medium was compared to that of oocytes fertilized in a traditional medium, modified Krebs-Ringer bicarbonate medium (mKRB), Sperm penetration was not observed when polyvinyl alcohol was replaced with ESA in mR1ECM (mR1ECM-BSA); the incidence was improved only when the osmolarity in mR1ECM-BSA was increased to that in mKRB (310 mOsm) by addition of NaCl, The proportion of oocytes penetrated in mR1ECM-BSA with NaCl increased (71.6 +/- 6.9%), which was not different compared to that in mKRB (76.7 +/- 13.7%). High incidences of sperm penetration (88.8 +/- 4.1% to 93.1 +/- 5.1%) were also observed when NaCl concentration in mR1ECM-BSA was increased from 76.7 mM to 100-130 mM. The incidence of embryos developing to the morula and blastocyst stages was higher when fertilized in mR1ECM-BSA containing 110-130 mM NaCl (91-94%) than in mKRB (70%), A total of 5 offspring were obtained after transfer of the morulae and blastocysts (69 embryos/ 7 females), These results demonstrate that a high developmental ability of rat embryos to the blastocyst stage is attained when the embryos have been fertilized in mR1ECM-BSA containing 110-130 mM NaCl and then cultured in mR1ECM.

DOI： 10.1095/biolreprod59.4.884

Web of Science

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Both bovine oocytes and cumulus cells produce plasminogen activator during in vitro maturation

SH Choi, H Funahashi, K Niwa

THERIOGENOLOGY 49 ( 1 ) 309 - 309 1998.1

Both bovine oocytes and cumulus cells produce plasminogen activator during in vitro maturation

SH Choi, H Funahashi, K Niwa

THERIOGENOLOGY 49 ( 1 ) 309 - 309 1998.1

Viability of boar spermatozoa frozen in 0.5ml-plastic straw by 2-step freezing method(共著)

Proceedings of the 8th World Conference on Animal Production, Contributed Papers 2 252 - 253 1998

Recent Development in Embryo Technology in Pigs. (共著)

Proceedings of the 8th World Conference on Animal Production, Symposium Series 2 240 - 251 1998

Cytoplasmic maturation of porcine oocytes for successful male pronuclear formation and early embryonic development

87 205 - 214 1998

Co-culture of bovine cumulus-oocyte complexes with theca cells inhibits the meiotic resumption at a specific phase of germinal vesicle.

KW Park, H Funahashi, K Niwa

BIOLOGY OF REPRODUCTION 58 ( Suppl.1 ) 219 - 219 1998

Intracellular calcium release and cortical reaction after thimerosal-treatment of porcine oocytes matured in vitro or ovulated.

H Funahashi, Z Machaty, WH Wang, TC Cantley, RS Prather, BN Day

BIOLOGY OF REPRODUCTION 58 ( Suppl.1 ) 219 - 219 1998

Inhibiting effect of theca cells on meiotic resumption of bovine oocytes may not be via cumulus cells(共著)

Proceedings of the 8th World Conference on Animal Production, Contributed PApers 2 920 - 921 1998

Effects of cumulus cells on the ability of pig oocytes to utilize cysteine or cystine during maturation in vitro

Ken Sawai, Hiroaki Funahashi, Koji Niwa

Journal of Reproduction and Development 44 ( 2 ) 161 - 168 1998

Language：English Publisher：The Japanese Society of Animal Reproduction (JSAR)

The present study examined the effect of cysteine and cystine associated with cumulus cells in maturation medium on oocyte glutathione (GSH) concentration at the end of maturation culture (for 48 h) and male pronuclear (MPN) formation following in vitro fertilization of pig oocytes. When cumulus-enclosed oocytes were cultured in a serum-free medium containing 0.57 mM cysteine or cystine, their GSH concentration and incidence of MPN formation were higher than when they were cultured without cysteine and cystine. Supplementation with cysteine during the final 24 h of culture resulted in an increased GSH concentration of oocytes and a higher incidence of MPN formation, regardless of the presence of cumulus cells, than in those cultured without cysteine or cystine. The same high incidence of MPN formation was observed even when oocytes were denuded after 36 h of culture and exposed to cysteine for only 12 h. In contrast, when oocytes were denuded after 24 h of culture and exposed to cystine, neither GSH synthesis nor MPN formation was improved, but both parameters did improve when oocytes were not denuded. Exposure of cumulus-enclosed oocytes to cystine from 36 h of culture did not promote MPN formation. These results indicate that cumulus-free pig oocytes can synthesize GSH in the presence of cysteine and that the presence of cumulus cells is essential for maintaining a high concentration of GSH in oocytes in the presence of cystine.

DOI： 10.1262/jrd.44.161

Scopus

CiNii Article

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Effects of cumulus cells on the ability of pig oocytes to utilize cysteine or cystine during maturation in vitro

Ken Sawai, Hiroaki Funahashi, Koji Niwa

Journal of Reproduction and Development 44 ( 2 ) 161 - 168 1998

Language：English Publisher：The Japanese Society of Animal Reproduction (JSAR)

DOI： 10.1262/jrd.44.161

Scopus

CiNii Article

researchmap

Recent Development in Embryo Technology in Pigs. (共著)

Proceedings of the 8th World Conference on Animal Production, Symposium Series 2 240 - 251 1998

Cytoplasmic maturation of porcine oocytes for successful male pronuclear formation and early embryonic developmentCytoplasmic maturation of porcine oocytes for successful male pronuclear formation and early embryonic development

87 205 - 214 1998

Viability of boar spermatozoa frozen in 0.5ml-plastic straw by 2-step freezing method(共著)

Proceedings of the 8th World Conference on Animal Production, Contributed Papers 2 252 - 253 1998

In vitro production of porcine embryos: On the developmental competence.

舟橋弘晃

Journal of reproduction and development 44 ( 6 ) j47-j52 - j52 1998

受精促進ペプチド(FPP)およびアデノシンの豚射出精液に及ぼす自発的先体反応阻止作用における競合(共著)

浅野敦之, 舟橋弘晃, 丹羽こう二, FRASER L R

第91回日本繁殖生物学会講演要旨集 91st ( 6 ) 45 - a24 1998

リン酸によるラット初期胚の発生阻害時期とマイクロフィラメント構造への影響(共著)

呉世薫, 舟橋弘晃, 丹羽こう二

第91回日本繁殖生物学会講演要旨集 91st ( 6 ) 43 - a22 1998

Co-culture of bovine cumulus-oocyte complexes with theca cells inhibits the meiotic resumption at a specific phase of germinal vesicle.

KW Park, H Funahashi, K Niwa

BIOLOGY OF REPRODUCTION 58 ( Suppl.1 ) 219 - 219 1998

Intracellular calcium release and cortical reaction after thimerosal-treatment of porcine oocytes matured in vitro or ovulated.

H Funahashi, Z Machaty, WH Wang, TC Cantley, RS Prather, BN Day

BIOLOGY OF REPRODUCTION 58 ( Suppl.1 ) 219 - 219 1998

Inhibiting effect of theca cells on meiotic resumption of bovine oocytes may not be via cumulus cells(共著)

Proceedings of the 8th World Conference on Animal Production, Contributed PApers 2 920 - 921 1998

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