MISC - FUNAHASHI Hiroaki

Experiment on transplantation of frozen in vitro fertilized bovine ova obtained under a complete in vitro culture system.

青柳敬人, 武富敏郎, 舟橋弘晃

JA全農飼料畜産中央研究所試験研究報告   ( 20(1991) )   1992

　More details

J-GLOBAL

researchmap

Experiment on production of in vitro fertilized bovine ova with donor identification.

青柳敬人, 舟橋弘晃, 武田哲男

JA全農飼料畜産中央研究所試験研究報告   ( 20(1991) )   1992

　More details

J-GLOBAL

researchmap

Study on repeated superovulation treatment of a cow using a corpus luteum hormone preparation.

青柳敬人, 武富敏郎, 板倉はつえ, 亀山賢次, 舟橋弘晃, 武田哲男, 鬼原辰夫

JA全農飼料畜産中央研究所試験研究報告   ( 20(1991) )   1992

　More details

J-GLOBAL

researchmap

1ST CLEAVAGE OF ENUCLEATED RAT EGGS FOLLOWING TRANSPLANTATION OF KARYOPLAST REMOVED FROM PRONUCLEAR EGGS STORED AT 2-DEGREES-C TO 6-DEGREES-C FOR VARIOUS DURATIONS

K NIWA, H FUNAHASHI, K OHMAE, M KATTOH

THERIOGENOLOGY   36 ( 3 )   411 - 417   1991.9

　More details

Language：English   Publisher：BUTTERWORTH-HEINEMANN  

Pronuclear rat eggs were cultured for 24 to 48 hours at 37-degrees-C after storage at 2 to 6-degrees-C for 0 to 216 hours in medium. Very high proportions (89 to 97%) of eggs cleaved to the two-cell stage after storage for 0 to 48 hours. The proportion, however, decreased rapidly in eggs stored for 72 hours (60%), and eggs stored for more than 120 hours cleaved poorly. However, when male and female pronuclei from stored eggs were transplanted into enucleated fresh eggs, 92 to 100% of the fused eggs with karyoplast stored for 0 to 144 hours cleaved. Although the cleavage rate was reduced to 50% when karyoplast from eggs stored for 168 hours was transplanted, this reduction was not significant. Complete loss of cleaving ability was observed in fused eggs with the karyoplast stored for 216 hours. These results clearly indicate that the pronuclei of rat eggs can be stored for a longer period than the cytoplasm at low temperatures (2 to 6-degrees-C).

DOI： 10.1016/0093-691X(91)90469-T

Web of Science

Scopus

researchmap

1ST CLEAVAGE OF ENUCLEATED RAT EGGS FOLLOWING TRANSPLANTATION OF KARYOPLAST REMOVED FROM PRONUCLEAR EGGS STORED AT 2-DEGREES-C TO 6-DEGREES-C FOR VARIOUS DURATIONS

K NIWA, H FUNAHASHI, K OHMAE, M KATTOH

THERIOGENOLOGY   36 ( 3 )   411 - 417   1991.9

　More details

Language：English   Publisher：BUTTERWORTH-HEINEMANN  

Pronuclear rat eggs were cultured for 24 to 48 hours at 37-degrees-C after storage at 2 to 6-degrees-C for 0 to 216 hours in medium. Very high proportions (89 to 97%) of eggs cleaved to the two-cell stage after storage for 0 to 48 hours. The proportion, however, decreased rapidly in eggs stored for 72 hours (60%), and eggs stored for more than 120 hours cleaved poorly. However, when male and female pronuclei from stored eggs were transplanted into enucleated fresh eggs, 92 to 100% of the fused eggs with karyoplast stored for 0 to 144 hours cleaved. Although the cleavage rate was reduced to 50% when karyoplast from eggs stored for 168 hours was transplanted, this reduction was not significant. Complete loss of cleaving ability was observed in fused eggs with the karyoplast stored for 216 hours. These results clearly indicate that the pronuclei of rat eggs can be stored for a longer period than the cytoplasm at low temperatures (2 to 6-degrees-C).

Web of Science

researchmap

DEVELOPMENTAL CAPACITY OF BOVINE OOCYTES COLLECTED FROM OVARIES OF INDIVIDUAL HEIFERS AND FERTILIZED INVITRO

H FUNAHASHI, Y AOYAGI, T TAKEDA, T ONIHARA

THERIOGENOLOGY   36 ( 3 )   427 - 434   1991.9

　More details

Language：English   Publisher：BUTTERWORTH-HEINEMANN  

Bovine follicular oocytes from individual heifers (n = 49) were separately matured, fertilized with frozen-thawed spermatozoa and cultured with cumulus cells. Although there were great variations in the number (mean +/- SD = 19.1 +/- 11.9) of oocytes collected from individual heifers and the percentages of the oocytes cleaved 48 hours after insemination (mean +/- SD = 69.5 +/- 18.4) and developed to the morula stage 7 days after insemination (mean +/- SD = 10.9 +/- 10.9), there were significant correlations between the numbers of oocytes collected and cleaved (the correlation coefficient: r = 0.9336) or developed to morula stage (r = 0.6633), indicating that oocytes from different heifers have the same developmental ability after in vitro fertilization. Ten morulae and 12 blastocysts which were obtained 7 and 8 days after insemination were transferred, one by one, to each uterine horn of 11 recipients. At Day 60 of pregnancy, 8 (80%) fetuses were identified in 4 (80%) of 5 recipients into which 10 embryos were transferred at Day -1 of synchrony. However, only 3 (25%) fetuses were identified in 2 (40%) of 6 recipients into which 12 embryos were transferred at Day 0 or +1 of synchrony.

DOI： 10.1016/0093-691X(91)90471-O

Web of Science

Scopus

researchmap

DEVELOPMENTAL CAPACITY OF BOVINE OOCYTES COLLECTED FROM OVARIES OF INDIVIDUAL HEIFERS AND FERTILIZED INVITRO International journal

H FUNAHASHI, Y AOYAGI, T TAKEDA, T ONIHARA

THERIOGENOLOGY   36 ( 3 )   427 - 434   1991.9

　More details

Language：English   Publisher：BUTTERWORTH-HEINEMANN  

Bovine follicular oocytes from individual heifers (n = 49) were separately matured, fertilized with frozen-thawed spermatozoa and cultured with cumulus cells. Although there were great variations in the number (mean +/- SD = 19.1 +/- 11.9) of oocytes collected from individual heifers and the percentages of the oocytes cleaved 48 hours after insemination (mean +/- SD = 69.5 +/- 18.4) and developed to the morula stage 7 days after insemination (mean +/- SD = 10.9 +/- 10.9), there were significant correlations between the numbers of oocytes collected and cleaved (the correlation coefficient: r = 0.9336) or developed to morula stage (r = 0.6633), indicating that oocytes from different heifers have the same developmental ability after in vitro fertilization. Ten morulae and 12 blastocysts which were obtained 7 and 8 days after insemination were transferred, one by one, to each uterine horn of 11 recipients. At Day 60 of pregnancy, 8 (80%) fetuses were identified in 4 (80%) of 5 recipients into which 10 embryos were transferred at Day -1 of synchrony. However, only 3 (25%) fetuses were identified in 2 (40%) of 6 recipients into which 12 embryos were transferred at Day 0 or +1 of synchrony.

Web of Science

PubMed

researchmap

黄体ホルモン製剤を用いた黒毛和種牛の反復過剰排卵処理に関する検討.(共著)

北海道牛受精卵移植研究会会報   ( 10 )   19 - 21   1991

　More details

researchmap

ラット前核期胚および桑実期胚の低温保存におよぼすショ糖の影響.(共著)

家畜繁殖学雑誌   37 ( 4 )   a21   1991

　More details

researchmap

Experiment on intergeneric muclear transplantation between early-stage embryos of rats and mice.

舟橋弘晃, 武田哲男, 丹羽こう二

JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

　More details

J-GLOBAL

researchmap

Study on nuclear transplantation in cattle.

亀山賢次, 青柳敬人, 舟橋弘晃, 武田哲男, 武富敏郎, 板倉はつえ

JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

　More details

J-GLOBAL

researchmap

Study n a low-cost narcotic method for pig.

武富敏郎, 亀山賢次, 舟橋弘晃, 板倉はつえ

JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

　More details

J-GLOBAL

researchmap

Experiment on developemtn in vivo of rat and mouse embryos produced by intergeneric nuclear transplantation.

舟橋弘晃, 武田哲男, 丹羽こう二

JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

　More details

J-GLOBAL

researchmap

Experiment on transplantation of frozen bovine embryo splits filled in artificial zona pellucida.

武田哲男, 青柳敬人, 亀山賢次, 武富敏郎, 舟橋弘晃, 板倉はつえ, 鬼原辰夫

JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

　More details

J-GLOBAL

researchmap

Experiment on trasplantaion of bovine embryos produced by in vitro fertilization those were developed under a complete in vitro culture system.

舟橋弘晃, 武富敏郎

JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

　More details

J-GLOBAL

researchmap

ラット2-細胞期胚における電気的割球融合

舟橋 弘晃, 丹羽 皓二

家畜繁殖学雑誌   36 ( 2 )   114 - 119   1990.6

　More details

Language：English   Publisher：(公社)日本繁殖生物学会  

researchmap

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=1990&ichushi_jid=J00240&link_issn=&doc_id=19870045330007&doc_link_id=10.1262%2Fjrd1977.36.114&url=https%3A%2F%2Fdoi.org%2F10.1262%2Fjrd1977.36.114&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

Electric fusion of 2-cell rat embryo balastomeres.

FUNAHASHI Hiroaki, NIWA Koji

Journal of Reproduction and Development   36 ( 2 )   114 - 119   1990

　More details

Language：English   Publisher：THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

Two-cell rat embryos were exposed to one or a train of two electric pulses with different field strengths and durations. It was difficult to induce blastomere fusion with a field strength of 1.0 kV/cm of 50μs duration: only 1 (7%) of 15 treated embryos fused after either one or a train of two pulses. The rate of fusion reached a maximum (80-100%) with 1.5-2.0 kV/cm and with 100-200μs duration. While the fusion rates were improved when the embryos were treated with a train of two pulses of 1.0 kV/cm for 100-200 μs, little effect was observed with 1.5 and 2.0 kV/cm. With 1.0 and 1.5 kV/cm, the fusion times were shortened when the embryos were exposed to a train of two pulses of 50 to 200 μs duration; no marked shortening of the time by the increased number of pulses was observed with 2.0 kV/cm. When fused embryos were transferred into pseudopregnant females and recovered 3 days later, 15 (83%), 12 (80%) and 9 (64%) of recovered embryos, which were originally submitted to 1.0, 1.5 and 2.0 kV/cm of field strengths each for 150 μs, respectively, had developed to the morula-blastocyst stage. There were no significant differences in the rates of development among these different field strengths.

DOI： 10.1262/jrd1977.36.114

CiNii Article

researchmap

牛体外受精卵の凍結保存におよぼす凍結保護物質の影響.(共著)

舟橋弘晃, 板倉はつえ, 武田哲男, 鬼原辰夫

第83回日本畜産学会大会講演要旨   83rd   15   1990

　More details

J-GLOBAL

researchmap

平成元年度野外実証試験について.

第15回農協畜産技術研究会講演要旨   103 - 107   1990

　More details

researchmap

Study of on cryoprotectant materials for split embryos of cattle and mouse.

武田哲男, 亀山賢次, 武富敏郎, 舟橋弘晃, 板倉はつえ

JA全農飼料畜産中央研究所試験研究報告   ( 18(1989) )   1990

　More details

J-GLOBAL

researchmap