Faculty Profiles - FUNAHASHI Hiroaki

MISC - FUNAHASHI Hiroaki

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Premature chromosomal condensation of nuclei transplanted into in vitro matured porcine oocytes.(共著)

Biology of Reproduction 48 ( Supplement 1 ) 175 1993

Pronuclear formation and in vitro development folowing artificial activation of pig oocytes matured different culture media.(共著)

Biology of Reproduction 48 ( Supplement 1 ) 171 1993

Assessment of porcine oocytes using brilliant cresyl blue

ERICSSON S

Theriogenology 39 ( 1 ) 214 - 214 1993

ラット前核期胚の体外発生におよぼすリン酸およびグルコ-スの影響.(共著)

三好和睦, 丹羽こう二, 奥田潔, 舟橋弘晃

家畜繁殖学会講演要旨 39 ( 5 ) a13 1993

Cytoplasmic maturation and in vitro development of porcine oocytes matured in Whitten's medium and fertilized in vitro.(共著)

Fourth International Conference on Pig Reproduction 36 1993

Developmental ability of porcine oocytes matured and fertilized in vitro.(共著)

Fourth International Conference on Pig Reproduction 23 1993

Effects of hormonal conditions during last 20 h of in vitro maturation on cytoplasmic maturation and vitro fertilization of pig oocytes.(共著)

Journal of Animal Science 71 ( Supplement 1 ) 70 1993

Pig oocyte activation and processing of transplanted nuclei.(共著)

39 ( 1 ) 329 1993

Electrical stimulation of pig pig oocytes in the presence or absence of calcium and magnesium.(共著)

Fourth International Conference on Pig Reproduction 42 1993

Processing of nuclei transplanted into in vitro matured porcine oocytes.(共著)

Theriogenology 39 ( 1 ) 322 1993

Effects of artificial activation before in vitro fertilization on sperm penetration and pronuclear formation in pig oocytes.(共著)

Theriogenology 39 ( 1 ) 222 1993

Developmental ability of porcine oocytes matured and fertilized in vitro.(共著)

Fourth International Conference on Pig Reproduction 23 1993

Electrical stimulation of pig pig oocytes in the presence or absence of calcium and magnesium.(共著)

Fourth International Conference on Pig Reproduction 42 1993

Cytoplasmic maturation and in vitro development of porcine oocytes matured in Whitten's medium and fertilized in vitro.(共著)

Fourth International Conference on Pig Reproduction 36 1993

FERTILIZATION AND EARLY CLEAVAGE INVITRO OF AGING BOVINE OOCYTES AFTER MATURATION IN CULTURE International journal

RC CHIAN, H NAKAHARA, K NIWA, H FUNAHASHI

THERIOGENOLOGY 37 ( 3 ) 665 - 672 1992.3

Language：English Publisher：BUTTERWORTH-HEINEMANN

Bovine follicular oocytes cultured for 20 to 48 hours were inseminated with frozen thawed spermatozoa. Significantly higher penetration rates (94 to 100% vs 86 to 94%: P < 0.05) and proportions of polyspermy (35 to 78% vs 22 to 45%: P < 0.01) were obtained for oocytes cultured continuously for 24 hours with spermatozoa than for those separated from spermatozoa 8 hours after insemination. The most prominent effect of ageing of oocytes on early events of penetration was observed in the incidence of polyspermy rather than in the penetration rate and the proportion of pronuclear plus cleaved oocytes: the proportion of polyspermic oocytes significantly increased (P < 0.05) in oocytes inseminated after 28 to 48 hours of culture (36 to 78%) compared with those cultured for 20 to 24 hours (22 to 35%) for maturation. Culture experiments for early development of penetrated oocytes indicated that no significant differences were observed in the proportions of oocytes cleaved to the two- to four- cell stage 48 hours after insemination among those cultured for 20 to 40 hours for maturation. However, further cleavage to the four- to sixteen-cell stage 72 to 96 hours after insemination was greatly inhibited as ageing of oocytes proceeded from 28 hours in culture for maturation.

DOI： 10.1016/0093-691X(92)90146-I

FERTILIZATION AND EARLY CLEAVAGE INVITRO OF AGING BOVINE OOCYTES AFTER MATURATION IN CULTURE

RC CHIAN, H NAKAHARA, K NIWA, H FUNAHASHI

THERIOGENOLOGY 37 ( 3 ) 665 - 672 1992.3

Language：English Publisher：BUTTERWORTH-HEINEMANN

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体外受精由来和牛胚の凍結融解後の生存性ならびに移植・分娩成績について.(共著)

北海道牛受精卵移植研究会会報 ( 11 ) 19 - 21 1992

Developmental abilities of mouse and rat pronuclear eggs following interspecific pronuclear and cytoplasmic transplantation.(共著)

Journal of Reproduction and Development 38 ( 3 ) 179 - 183 1992

Developmental Abilities of Rat and Mouse Pronuclear Eggs Following Interspecific Nuclear or Cytoplasmic Transplantation

Hiroaki Funahashp, Koji Niwa

Journal of Reproduction and Development 38 ( 3 ) 179 - 183 1992

Language：English

Homologous and heterologous replacement of a small amount of cytoplasm or both pronuclei were performed in rat and mouse pronuclear eggs. Rat eggs after receiving rat or mouse cytoplast never developed beyond the 2-cell stage in vitro. On the other hand, the in-vitro development of mouse eggs that received rat cytoplasm was possible, but it was greatly inhibited compared with those that received mouse cytoplasm. None of enucleated rat eggs that received rat or mouse pronuclei also developed beyond the 2-cell stage. Although few enucleated mouse eggs after receiving rat pronuclei developed beyond the 2-cell stage, high proportion (64%) of those that received mouse pronuclei could develop to blastocysts in vitro. When 2-cell embryos obtained 18 h after fusing with donor karyoplast were transferred to pseudopregnant rats, 2 (9%) of 23 enucleated rat embryos and 1 (7%) of 15 enucleated mouse embryos each fused with heterologous karyoplast developed to early blastocyst 48-55 h after transfer. These results may indicate that the embryonic genome can be activated in the heterospecific cytoplasm. © 1992, THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT. All rights reserved.

DOI： 10.1262/jrd.38.179

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牛体外受精卵の初期発生におよぼすグルコースの影響

金宗興, 舟橋弘晃, 伊賀浩輔, 丹羽こう二

日本畜産学会大会講演要旨 85th 1992

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