研究者詳細 - 最相　大輔

MISC - 最相　大輔

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Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions

M. Nakazono, H. Tsuji, Y. Li, D. Saisho, S. I. Arimura, N. Tsutsumi, A. Hirai

Plant Physiology 124 ( 2 ) 587 - 598 2000年

記述言語：英語出版者・発行元：American Society of Plant Biologists

It is known that alcoholic fermentation is important for survival of plants under anaerobic conditions. Acetaldehyde, one of the intermediates of alcoholic fermentation, is not only reduced by alcohol dehydrogenase but also can be oxidized by aldehyde dehydrogenase (ALDH). To determine whether ALDH plays a role in anaerobic metabolism in rice (Oryza sativa L. cv Nipponbare), we characterized a cDNA clone encoding mitochondrial ALDH from rice (Aldh2a). Analysis of sub-cellular localization of ALDH2a protein using green fluorescent protein and an in vitro ALDH assay using protein extracts from Escherichia coli cells that overexpressed ALDH2a indicated that ALDH2a functions in the oxidation of acetaldehyde in mitochondria. A Southern-blot analysis indicated that mitochondrial ALDH is encoded by at least two genes in rice. We found that the Aldh2a mRNA was present at high levels in leaves of dark-grown seedlings, mature leaf sheaths, and panicles. It is interesting that expression of the rice Aldh2a gene, unlike the expression of the tobacco (Nicotiana tabacum) Aldh2a gene, was induced in rice seedlings by submergence. Experiments with ruthenium red, which is a blocker of Ca2+ fluxes in rice as well as maize (Zea mays), suggest that the induction of expression of Adh1 and Pdc1 by low oxygen stress is regulated by elevation of the cytosolic Ca2+ level. However, the induction of Aldh2a gene expression may not be controlled by the cytosolic Ca2+ level elevation. A possible involvement of ALDH2a in the submergence tolerance of rice is discussed.

DOI： 10.1104/pp.124.2.587

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冠水条件下でのイネミトコンドリア遺伝子の発現応答 2. アルデヒド脱水素酵素(ALDH)遺伝子の発現誘導

中園幹生, 辻寛之, 最相大輔, 中園江, 堤伸浩, 平井篤志

育種学研究 1 76 1999年4月

冠水条件下でのイネミトコンドリア遺伝子の発現応答 1. 呼吸系遺伝子の発現

辻寛之, 中園幹生, 最相大輔, 堤伸浩, 平井篤志

育種学研究 1 75 1999年4月

Expression of the alternative oxidase gene is induced by treatment of rice with the bleaching herbicide norflurazon

Daisuke Saisho, Mikio Nakazono, Nobuhiro Tsutsumi, Atsushi Hirai

RICE GENET. NEWSLT. 1999年

CYCLOHEXIMIDE INDUCES TRANSCRIPTION OF THE GENE FOR ALTERNATIVE OXIDASE IN Arabidopsis thaliana

SAISHO Daisuke, TSUTSUMI Nobuhiro, HIRAI Atsushi, NAKAZONO Mikio

Plant and cell physiology 39 S94 - S94 1998年5月

Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature.(共著)

GENE 203 ( 2 ) 121 - 129 1997年12月

Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature

Yusuke Ito, Daisuke Saisho, Mikio Nakazono, Nobuhiro Tsutsumi, Atsushi Hirai

Gene 203 ( 2 ) 121 - 129 1997年12月

記述言語：英語

We identified two genes for alternative oxidase (AOX) from rice. One AOX gene (designated AOX1a) is located approx. 1.9 kb downstream of another AOX gene (designated AOX1b). Comparison of the genomic and cDNA sequences of the two AOX genes showed that the AOX1a gene is interrupted by three introns, as are AOX genes of other plants. On the other hand, two introns are inserted in the AOX1b gene. The predicted AOX1a and AOX1b precursor proteins consist of 332 and 335 amino acid residues, respectively. A genomic Southern hybridization analysis indicated that rice has several AOX genes other than the two tandem-arranged AOX genes. Steady-state mRNA levels of both of the genes for AOX1a and AOX1b were increased under low temperature (4°C). However, no difference in the pattern of induction of transcription between the genes for AOX1a and AOX1b was observed.

DOI： 10.1016/S0378-1119(97)00502-7

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Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature. (共著)

GENE 203 ( 2 ) 121 - 129 1997年12月

Characterization of the gene family for alternative oxidase from Arabidopsis thaliana.(共著)

Plant Mol. Biol. 35 ( 5 ) 585 - 596 1997年11月

Characterization of the gene family for alternative oxidase from Arabidopsis thaliana

Daisuke Saisho, Eiji Nambara, Satoshi Naito, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono

Plant Molecular Biology 35 ( 5 ) 585 - 596 1997年11月

記述言語：英語

We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a, AOX1b, AOX1c and AOX2) of the AOX genes. Interestingly, two of the AOX genes (AOX1a and AOX1b) were located in tandem in a ca. 5 kb region on one of the chromosomes of Arabidopsis. Comparison between genomic and cDNA sequences of the four AOX genes showed that all AOX genes are divided by three introns and the positions of the introns in AOX1a, AOX1b, AOX1c and AOX2 are the same. We examined whether expression of Arabidopsis AOX genes, like the tobacco AOX1a gene, is enhanced by treatment with antimycin A, an inhibitor of complex III in the mitochondrial respiratory chain. We found that, in young plants, the amount of Arabidopsis AOX1a mRNA was dramatically increased by addition of antimycin A, while the transcription of the other three genes (AOX1b, AOX1c and AOX2) did not respond to antimycin A. Amplification by RT-PCR showed that AOX1a and AOX1c were expressed in all organs examined (flowers and buds, stems, rosette, and roots of 8-week old plants). In contrast, transcripts of AOX1b were detected only in the flowers and buds, and transcripts of AOX2 were detected mainly in stems, rosette and roots. These results suggested that transcriptions of the four genes for alternative oxidase of Arabidopsis are differentially regulated.

DOI： 10.1023/A:1005818507743

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Two tandemly-repeated genes for alternative oxidase from Arabidopsis thaliana are differentially expressed.

M Nakazono, D Saisho, S Naito, N Tsutsumi, A Hirai

PLANT PHYSIOLOGY 114 ( 3 ) 1014 - 1014 1997年7月